Yuav tag nrho glycoside los ntawm cistanche inhibit daim tawv nqaij pigmentation thiab whitening tawv nqaij xim?
Mar 12, 2022
Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791
Cistancheyog ib qho tshuaj suav tshuaj thiab muaj nqi heev. Nws cov tshuaj yog nws qhuav fleshy qia nrog nplai thiab nplooj.CistancheFeem ntau yog tsim nyob rau hauv Xinjiang, faib nyob rau hauv ob sab qaum teb thiab yav qab teb Xinjiang, thiab yog lub ntsiab ua cheeb tsam ntawm Cistanche.Tag nrho Glycosides ntawm Cistancheyog ib chav kawm ntawmPhenylethanol GlycosidesCov tshuaj tau muab rho tawm thiab cais tawm ntawm cov nroj tsuag hauv genus Cistanche. Nws yog qhov tseem ceeb ntawm cov nroj tsuag hauv genus Cistanche. Tam sim no, ze li ntawm 70 monomer Cheebtsam tau raug cais tawm los ntawm cov nroj tsuag hauv genus (Liu Guojun, editor hauv tus thawj coj. Cistanche Thiab cog khoom cuav. China Labor thiab Social Security Press, 2003.120). Muaj ntau pua cov ntaub ntawv tshawb fawb txogTag nrho Glycosides ntawm Cistanchenyob rau hauv tsev thiab txawv teb chaws, tab sis tsis muaj leej twg ntawm lawv koom nrog cov nyhuv ntawm inhibiting ntawm daim tawv nqaij pigmentation thiab whitening kev zoo nkauj [Deng Yan li al. Kev tshawb nrhiav kev nce qib ntawm kev tswj hwm ntawm melanocytes thiab tyrosinase los ntawm cov tshuaj suav. New Chinese Medicine, 2003,35(5):72-73]o Lub tswv yim tam sim no siv Glycoside los ntawmcistanche tubulosaraws li cov khoom tshawb fawb thiab siv tyrosinase dopa tus nqi oxidation txoj kev los kawm nws cov tyrosinase inhibitory nyhuv. Cov txiaj ntsig qhia tau tiasTag nrho Glycosides ntawm Cistanchemuaj qhov cuam tshuam tseem ceeb ntawm kev ua haujlwm ntawm tyrosinase, uas qhia tiastag nrho Glycosides ntawm Cistanchemuaj cov nyhuv inhibitingdaim tawv nqaij pigmentationthiabtawv nqaij dawbteebmeem.
Cistanche inhibits daim tawv nqaij pigmentation
Tyrosinase yog qhov tseem ceeb ntawm tus nqi txwv enzyme hauv cov txheej txheem ntawmtawv nqaijmelaninbiosynthesis. Nws yog ib qho complex ntawm tooj liab thiab protein. Nws tuaj yeem hydroxylate tyrosine, cov khoom siv tseem ceeb rau melanin ntau lawm hauv lub cev, tsim L-dopa, uas tom qab ntawd oxidized rau dopa dai. Dopa dai tau dhau los ua cov txheej txheem metabolic thiab thaum kawg ua ke nrog cov protein los tsim melanin. Qhov tseem ceeb tshaj plaws ntawm cov xim ntawm daim tawv nqaij, kev sib txuam ntau dhau tuaj yeem ua rau cov kab mob hyperpigmented ntawm daim tawv nqaij xws li freckles, chloasma, hnub nyoog melanoma, thiab melanoma. Yog li ntawd, los ntawm kev tshawb fawb ntawm inhibition ntawm tyrosinase kev ua, cov khoom xyaw zoo uasinhibit daim tawv nqaij pigmentationtuaj yeem raug tshuaj xyuas, uas tuaj yeem siv rau kev tiv thaiv kab mob ntawm daim tawv nqaij hyperpigmented thiabtawv nqaijntxuav hniav dawbthiab freckle tshem tawm muaj nuj nqi ntawm cov tshuaj pleev ib ce sab nraud.
Summary ntawm invention:
Lub hom phiaj ntawm qhov kev tsim kho tam sim no yog los muab kev siv tshiabTag nrho Glycosides ntawm Cistanchelos yog ib qho extract uas muajPhenylethanol Glycosidesntawm Cistanche. Txoj kev siv yog los soj ntsuam cov nyhuv inhibitory ntawmTag nrho Glycosides ntawm Cistanchentawm tyrosinase los ntawm kev siv tyrosinase tus nqi oxidation txoj kev. Qhov tshwm sim yog qhov ntawdTag nrho Glycosides ntawm Cistanchemuaj qhov cuam tshuam tseem ceeb ntawm kev ua haujlwm ntawm tyrosinase, qhia tias nws tuaj yeem cuam tshuam cov tawv nqaij pigmentation Thiab whitening thiab kev zoo nkauj ua haujlwm, muab kev ua haujlwm tshiab rau Cistanche cov khoom kho mob thiab kev npaj tshuaj, thiab nthuav nws daim ntawv thov mus rau thaj chaw ntawm cov tshuaj pleev ib ce.
cistanchemuaj lub luag haujlwm ntawmtawv nqaij dawb
Txoj kev kuaj:
Txoj kev tshwj xeeb rau kev kuaj xyuas qhov tshwj xeeb absorbance tus nqi A490o ntawm cov tshuaj tiv thaiv cov khoom ntawm 490nm siv tyrosinase dopa tus nqi oxidation txoj kev nrog ib tug enzyme-txuas immunoassay yog raws li nram no:
1. Sib tov tyrosine (Tianjin Guangfu Fine Chemical Research Institute) rau hauv ib qho 0.02 feem pua tshuaj nrog 67mmol • L'* 12phosphate buffer (PB daws), thiab tyrosinase (2350U / mg, Sigma tuam txhab) rau hauv cov tshuaj 500U-mL'1 tshuaj, dais txiv hmab txiv ntoo co (Beijing Naike Biochemical Research Institute) yog ua rau 1.0mg•mL4daws.
2. Tag nrho Glycosides ntawm Cistanchetuaj ntawm Cistanche (Cista&che tubidosaR.Wizheng), ntsuas los ntawm ultraviolet spectrophotometry, thiab muaj ntau tshaj 50 feem pua ntawm phenethylamines. Npaj Tag Nrho Glycosides ntawm Cistanche nrog 67mmol • L'1phosphate buffer tov rau ib qho concentration ntawm {{0}}.5 ~ 3.0mg • mL'1.
Tag nrho Glycosides ntawm Cistanche
3. Cov qauv tsis muaj tshuaj tau siv los ua pawg tswj hwm dawb huv, thiab Xiong Guoyu tau siv los ua pawg tswj hwm zoo.
4. Cov tshuaj tiv thaiv yog nqa tawm nyob rau hauv ib tug 96- zoo kab lis kev cai phaj. Tag nrho cov tshuaj tiv thaiv yog 150lxL. Noj 50 nL ntawm cov tshuaj ntsuam xyuas thiab ntxiv 50 uL ntawm tyrosine. Tom qab sib tov tag nrho, sai sai ntxiv 50uL (25U) ntawm tyrosinase nyob rau hauv ib da dej dej khov thiab sib tov. Ib yam nkaus, incubate ntawm 25 degree rau 15min, thiab tam sim ntawd teem lub microplate nyeem ntawv ntsuas A490 hauv txhua qhov dej. Txhua pawg ntawm cov kev xeem sib npaug 5 zaug.
Cov txiaj ntsig kuaj:
Sib txawv concentrations ntawmTag nrho Glycosides ntawm Cistanchemuaj ntau theem ntawm inhibition ntawm tyrosinase, thiab tus nqi inhibition yog kwv yees li ntawm koob tshuaj-nyob ntawm cov koob tshuaj ntawm cov nyhuv hauv ib qho kev concentration ntau, uas tau qhia nyob rau hauv tib lub substrate concentration, Cov nyhuv inhibitory ntawmTag nrho Glycosides ntawm Cistanchentawm tyrosinase nce nrog nce concentration. Qhov concentration ntawmTag nrho Glycosides ntawm Cistancheyog nyob rau hauv qhov ntau ntawm {{0}}.5 ~ 3.0mg-mL-1, uas muaj cov nyhuv inhibitory ntawm tyrosinase kev ua, thiab muaj cov tshuaj zoo-zoo kev sib raug zoo nyob rau hauv ntau qhov concentration ntawm 0.5 ~ 2.0mg • mL; Tag nrho Glycosides ntawm Cistanche thiab nyob rau hauv tib lub concentration ntawm bearberry, cov nyhuv tsis zoo li cov bearberry, tab sis thaum lub concentration yog nce mus rau 1.5mg·mL, cov nyhuv yog muaj zog tshaj li bearberry. Tom qab qhov nruab nrab t-test ntawm ob qhov qauv, lubTag nrho Glycosides ntawm Cistanchepab pawg (ntseeg nyob rau hauv qhov ntau ntawm 1.0~3.0mg-mL'1) thiab cov tswj zoo bearberry alfalfa pawg tau muab piv nrog cov pab pawg dawb huv, thiab cov nyhuv inhibitory ntawm tyrosinase tau txheeb xyuas. tseem ceeb. Qhov tseem ceeb ntawm kev tshawb fawb, uas yog, Tag Nrho Glycosides ntawm Cistanche thiab bearberry smoothes muaj qhov cuam tshuam tseem ceeb ntawm kev ua haujlwm tyrosinase, tawm tswv yim tiasTag nrho Glycosides ntawm Cistanchemuaj cov nyhuv ntawminhibiting daim tawv nqaij pigmentationthiabtawv nqaij dawbcov teebmeem, thiab nws yuav raug tsim los ua kev kho mob rau hyperpigmentation yav tom ntej. Cov tshuaj dermatological thiab whitening thiab freckles tshem tawm cov khoom xyaw raws li cov tshuaj pleev ib ce sab nraud nteg lub hauv paus zoo.
Tag nrho Glycosides ntawm Cistanche inhibits daim tawv nqaij pigmentation thiab whiten ntawm daim tawv nqaij
