Temporal Endurance Of Exercise-induced Benefits On Hippocampus-dependent Memory Thiab Synaptic Plasticity nyob rau hauv Poj Niam nas Part 2
Oct 23, 2023
2.5. Hauv vitro hippocampal hlais kev npaj
Tsis ntev tom qab OLM tau txais, nas tau muab tshuaj loog nrog isoflurane, decapitated, thiab lub hlwb raug tshem tawm sai thiab submerged nyob rau hauv dej txias, oxygenated dissectionmedium uas muaj (hauv mM): 124 NaCl, 3 KCl, 1.25 KH 2 PO 4, 5 MgSO 4, 0 CaCl 2, 26NaHCO 3, thiab 10 qabzib.
Peb txhua tus paub tias qabzib yog lub hauv paus tseem ceeb ntawm lub zog, tab sis koj puas paub tias nws pab txhim kho kev nco?
Glucose yog qhov zoo rau lub hlwb ua haujlwm vim tias cov piam thaj yog lub hlwb tseem ceeb roj. Peb lub hlwb xav tau lub zog tas li los ua ntau yam haujlwm nyuaj xws li kev xav, kev paub, thiab kev nco. Raws li peb cov haujlwm niaj hnub nce, lub zog xav tau ntawm lub hlwb kuj nce ntxiv, yog li peb yuav tsum tau noj cov piam thaj kom txaus kom lub hlwb ua haujlwm.
Tsis tas li ntawd, cov kev tshawb fawb pom tau tias cov piam thaj tuaj yeem txhim kho lub hlwb lub peev xwm nco. Thaum ua cov haujlwm nco, lub hlwb xav tau cov piam thaj ntau los txhawb kev sib txuas lus thiab cov ntaub ntawv xa mus ntawm ntau cov neurons. Yog tias cov khoom noj qabzib tsis txaus, lub hlwb kev nco yuav raug cuam tshuam. Yog li ntawd, tau txais cov piam thaj txaus yog ib qho ntawm cov yuam sij rau kev noj qab haus huv lub hlwb.
Muaj ntau qhov chaw ntawm cov piam thaj, xws li cov nplej, txiv hmab txiv ntoo, zaub, thiab cov carbohydrates zoo. Peb tsuas yog yuav tsum tau noj cov zaub mov no kom tsim nyog hauv peb cov zaub mov noj txhua hnub kom tau txais cov piam thaj txaus, yog li txhawb kev loj hlob ntawm lub hlwb.
Hauv ntej, cov piam thaj yog ib qho tseem ceeb hauv kev tswj xyuas lub hlwb thiab tuaj yeem pab peb sau cov ntaub ntawv zoo dua. Yog li ntawd, kev noj zaub mov kom zoo thiab noj cov piam thaj kom txaus tsis yog tsuas yog zoo rau lub cev xwb, tab sis kuj tseem ceeb heev rau lub hlwb. Nws pom tau tias peb yuav tsum txhim kho peb lub cim xeeb. Cistanche deserticola tuaj yeem txhim kho kev nco, vim Cistanche deserticola tseem tuaj yeem tswj hwm qhov sib npaug ntawm cov neurotransmitters, xws li nce qib ntawm acetylcholine thiab kev loj hlob. Cov khoom no tseem ceeb heev rau kev nco thiab kev kawm. Tsis tas li ntawd, cov nqaij kuj tuaj yeem txhim kho cov ntshav khiav thiab txhawb nqa cov pa oxygen, uas tuaj yeem ua kom lub hlwb tau txais cov as-ham txaus thiab lub zog, yog li txhim kho lub hlwb tseem ceeb thiab kev ua siab ntev.
Nyem paub txoj hauv kev los txhim kho lub hlwb
Coronal hippocampal slices (340 μm) (n=11} (0–0–0), 6 (14–{8}}–{ {12}}), 10 (14–7–0), 8 (14–7–2) hlais los ntawm 6, 3, 5 thiab 5 nas) tau npaj siv Leicavibrating cov ntaub so ntswg (Model: VT1000S) ua ntej xa mus rau ib qho kev sib txuas cov ntaub ntawv uas muaj preheated dag cerebrospinal kua (CSF) ntawm cov nram qab no muaj pes tsawg leeg (inmM): 124 NaCl, 3 KCl, 1.25, KH 2 PO 4, 1.5 MgSO 4, 2.5 CaCl 2, 26 NaHCO 3, thiab 10glucose ± 1. 10 C. Slices tau txuas ntxiv perfused nrog cov tshuaj no ata tus nqi ntawm 1.75-2 ml / min thaum lub ntsej muag ntawm cov hlais tau raug sov, humidified 95% O2 / 5% CO2. Cov ntaub ntawv pib tom qab tsawg kawg 2 teev ntawm incubation.
Field excitatory postsynaptic muaj peev xwm (fEPSPs) tau sau tseg los ntawm CA1b stratumradiatum apical dendrites siv ib lub iav pipette uas muaj 2 M NaCl (2–3 MΩ) teb rau orthodromic stimulation (twisted nichrome hlau, 65 μmlateral txoj kab uas hla) ntawm SchmisafferCA stratum radiatum. Pulses tau tswj hwm ntawm 0.05 Hz siv qhov tam sim no uas ua rau 50% qhov siab tshaj plaws tsis muaj cov lus teb. Tom qab tsim 10–20-feeb hauv paus ruaj khov, lub sij hawm ntev potentiation (LTP) tau tshwm sim los ntawm kev xa tawm ib ntus ntawm 5 'theta' tawg, txhua qhov tawg muaj plaub pulses ntawm 100 Hz thiab qhov tawg lawv tus kheej sib cais los ntawm 200 ms ( piv txwv li, theta tawg stimulation los yog TBS). Qhov stimulationintensity tsis tau nce thaum TBS.
Cov nuj nqis raug nthuav tawm raws li txhais tau tias ± SEM. FEPSP txoj kab nqes tau ntsuas ntawm 10-90% kev poob ntawm txoj kab nqes thiab cov ntaub ntawv hauv cov duab ntawm LTP tau ua kom zoo li qub rau 20 feeb kawg ntawm lub hauv paus.Electrophysiological ntsuas tau txheeb xyuas siv ANOVA ib-txoj kev tshwj tsis yog muaj lwm yam hauv cov ntawv nyeem thiab theem ntawm qhov tseem ceeb tau teeb tsa ntawm p Tsawg dua lossis sib npaug rau 0.05.
2.6. RT-qPCR
Dorsal hippocampus cov ntaub so ntswg tau khov ntawm -80 degree kom txog thaum ua tiav. RNAwas cais siv RNeasy Mini Cov Khoom Siv (Qiagen) raws li cov chaw tsim khoom cov lus qhia thiab tag nrho RNA (50 ng) tau thim rov qab. cDNA synthesis tau ua tiav siv Cov Khoom Siv Muaj Peev Xwm CDNA Reverse Transcription (Roche AppliedScience). Primers tau tsim los siv Roche Universal Probe Library; tag nrho cov primerswere tau los ntawm IDT thiab sojntsuam los ntawm Roche Universal Probe Library thiab tau siv rau multiplexing hauv Roche Light-Cycle 480 II Machine (Roche).
Rau bdnf exon VI transcript thiab Hprt, peb tsim PrimeTime qPCR assay (IDT) vim tsis muaj Universal Probe Library xeem. Cov nram qab no primerswere siv: bdnf exon I: pem hauv ntej primer (5′–3′): GCATTGTTGGGGAGACAAG, rov qab primer (5′–3′): TCACCTGGTG-GAACATTGTG, sojntsuam 56, bdnfexon IV: pem hauv ntej primer (5′–3′) : GCTGCCTTGATGTTTTTTTGA, reverseprimer (5′–3′): AAGGATGGT-CATCACTCTTTCA, sojntsuam 31, bdnf exonVI: pem hauv ntej primer (5′–3′): CTGGGAAGGCTTTGATGAGAC, reverseprimer (5′–3′): GCCCTTAGATG- 56-FAM/AGA CAG/Zen/AACGGTGA-CAACAAT/3IABkFQ/; bdnf exon IX: pem hauv ntej primer (5′–3′): GCCTTTTGGAGCCTCCTCTAC, thim rov qab primer (5′–3′): AAGGATGGTCATCACTCTTTCTCA, sojntsuam 31. Txhua lub hom phiaj probes tau conjugated rau lub dyeFAM.
Tag nrho cov nqi tau normalized rau Hprt qhia, uas siv cov nram qab no primer: pem hauv ntej primer (5′–3′): TGCTCGAGATGTCATGAAGG, rov qab primer (5′–3′): CTTTTATGTCCCCCGTT-GAC, sojntsuam: /5HEX/AT CAC ATT G/Zen /T GGC CCT CTGT/3IABkFQ/. Lub normalization ntawm qhov tseem ceeb yog hloov mus rau Hprt qhia. Cov ntaub ntawv thiab statisticalanalysis yog ua tiav siv Roche proprietary algorithms thiab REST 2009 software raws li txoj kev Plaffl (Pfaffl, 2001; Pfaffl li al., 2002).
2.7. Kev txheeb cais
Cov qauv loj hauv qhov kev tshawb fawb no zoo ib yam li cov feem ntau siv hauv thaj chaw, suav nrog cov ntawv tshaj tawm hauv cov ntawv tshaj tawm dhau los (xws li Butler li al., 2019; Keizer et al., 2021; Kwapis et al., 2018, 2020 ; López li al., 2019; Vogel-Ciernia li al., 2013) Txawm hais tias tsis muaj cov txheej txheem txheeb cais tau siv los txiav txim qhov ntau thiab tsawg. Kev txheeb xyuas kev txheeb xyuas tau ua los ntawm ob txoj kev ANOVA (Figs.1, 2D, 3B, 4, S1C–F, S2 C–F) lossis ob txoj kev ANOVA (Figs. 2A–C, S1A–B, S2 A–B) ua raws li Tukey- kho t-tests los sib piv ib pawg neeg. Kev sib piv yooj yim rau kev ntsuam xyuas cov qhab nia ntawm kev ntxub ntxaug (DI) tau ua nyob rau hauv pab pawg los sib piv kev cob qhia thiab kev xeem DI uas siv Cov Tub Ntxhais Kawm T-test (Fig. 1D). Ob txoj kev ANOVAhad yam ntawm Estrous Phase thiab Exercise Condition (Fig. 2A–B), Estrous Phase thiab Hnub (Fig. 2C) lossis Exercise Condition and Day (Fig. S1 A–B, S2 A–B). Tag nrho cov txheeb cais tau ua tiav nrog GraphPad Prism 8 software. Cov teebmeem tseem ceeb thiab kev cuam tshuam rau txhua tus ANOVAare tau piav qhia hauv cov ntawv nyeem. Tag nrho cov kev ntsuam xyuas yog ob-tailed thiab xav tau tus nqi ntawm 0.05 forsignificance. Cov kab yuam kev hauv txhua daim duab sawv cev SEM.
3. Cov txiaj ntsig
3.1. Kev tawm dag zog ua kom muaj kev nco mus ntev nyob rau hauv qhov kev nkag mus rau cov xwm txheej hauv cov nas poj niam
Yav dhau los peb tau pom tias 14d kev tawm dag zog hauv cov txiv neej nas pab txhawb kev kawm nyob rau hauv subthreshold tau txais cov xwm txheej ntawm OLM txoj haujlwm uas tau tshem tawm tom qab 7d ntawm kev ncua qeeb (Butler li al., 2019). Ntxiv mus, lub subthreshold 2d ntawm reactivatingexercise tom qab no sedentary ncua sij hawm tuaj yeem rov koom nrog thawj cov txiaj ntsig ntawm 14d kev tawm dag zog los txhawb cov txiaj ntsig ntawm kev paub. Ntawm qib molecular, kev tawm dag zog-tsim hippocampalBDNF kuj tseem txo qis los ntawm 7 mus rau 14d kev tawm dag zog tom qab thiab tuaj yeem rov ua kom yooj yim dua nrog 2d kev sib tw tom ntej ntawm lub log khiav (Berchtold li al., 2005, Berchtold li al., 201, 201). qhov kev tawm dag zog lub cev muaj xws li 14d ntawm kev tawm dag zog thawj zaug, 7d ntawm sedentarydelay, thiab 2d ntawm kev tawm dag zog rov ua haujlwm zoo li ntes lub sijhawm lub cev ntawm kev tawm dag zog ntawm kev tawm dag zog hauv cov txiv neej.
Ntawd yog, qhov kev tawm dag zog thawj zaug koom nrog kev paub txog cov txiaj ntsig, uas txawm tias dormant thaum qoj ib ce, tuaj yeem rov ua haujlwm los ntawm kev tawm dag zog tom qab. Yog li ntawd, peb tshuaj xyuas seb qhov kev tawm dag zog zoo sib xws kuj koom nrog thiab tswj cov txiaj ntsig kev paub txog kev tawm dag zog hauv poj niam. Peb tau khiav poj niam nas los ntawm kev tawm dag zog sib txawv uas suav nrog kev tawm dag zog thawj zaug (14d lossis 0d) ua raws li kev ncua ncua sijhawm (7d lossis 0d) thiab kev tawm dag zog rov ua haujlwm (2d lossis 0d) ) ua ntej OLM acquisitionand test (Fig. 1A).
Txhawm rau soj ntsuam cov txiaj ntsig zoo ntawm kev tawm dag zog ntawm kev ua haujlwm ntawm lub cim xeeb, nas tau txais 3-feeb subthreshold tau txais kev sib ntsib thaum lub sijhawm qhov chaw sib txawv (A1 thiab A2) ntawm ob yam khoom zoo tib yam tau kawm. Hauv cov txiv neej nas, lub sijhawm kev cob qhia no tau pom tias yog ib qho kev hloov pauv rau cov tsiaj nyeg (Butler li al., 2019; Intlekofer li al., 2013; Malvaez li al., 2013; McQuown et al., 2011). Nyob rau hnub xeem, ib qho ntawm cov khoom nyob rau hauv thawj qhov chaw tau tsiv mus rau qhov chaw tshiab (A3) thiab lub sij hawm siv tshawb xyuas txhua qhov chaw khoom raug tshuaj xyuas. Muab cov nas tso rau hauv qhov kev nyiam nyob rau hauv innate rau novelty, lub sij hawm ntev OLM yog pov thawj los ntawm kev tshawb fawb ntau dua ntawm cov khoom tshiab qhov chaw piv rau cov khoom hauv qhov chaw ruaj khov.
Kev tawm dag zog tsis zoo cuam tshuam kev nco ua haujlwm rau hnub xeem (Daim duab 1D; ib-txoj kev ANOVA, Pab Pawg F (3,27)=7.66, p=0.{{10}} 007), qhov twg 14 hnub ntawm kev tawm dag zog thawj zaug (14–0–0) tau coj torobust mus sij hawm nco kev ua tau zoo txheeb ze rau pawg tswj sedentary ({{ 22}}–0–{28}}) (Fig.1D Tukey's post hoc test, p=0.003). Ib 7-hnub sedentary ncua sij hawm ua rau poob qis rau qhov kev ua tau zoo ntawm 14–0–0 thawj pab pawg ua haujlwm (Fig. 1D Tukey's post hoc test, p =0.007) thiab qhov tsis tseem ceeb sib txawv ntawm kev tswj (0–0–0) (Fig. 1D Tukey's post hoctest, p=0.985).
Txhawm rau tshawb xyuas seb cov txiaj ntsig ntawm qhov kev tawm dag zog thawj zaug puas tau khaws cia thoob plaws hauv lub sijhawm qeeb, ib pawg tsiaj tau raug rau 2d ntawm kev rov ua haujlwm ua ntej OLM tau txais tom qab kev ncua qeeb (14-7-2). Cov nas nyob rau hauv qhov kev tawm dag zog lub cev (14–7–2) tau pom ntau dua DI cov qhab nia piv rau ob qho tib si kev tswj hwm sedentary (0–0–0) (Fig. 1D Tukey's post hoc test, p=0.019) andsedentary delay (14–7–0) cohorts (Fig. 1D Tukey's post hoc test, p=0.043).
Nyob rau hauv-pab pawg sib piv (Daim duab 1D, qhov tseem ceeb suav nrog # cim hauv cov kab) ntawm DI cov qhab nia los ntawm hnub tau txais piv rau hnub xeem qhia tau ntau dua DI cov qhab nia thaum lub sij hawm xeem piv nrog rau kev kawm tsuas yog 14–0–{{4} } (t (12)=8.449, p=0.00{{20}}}1) thiab 14–7–2 pawg (t (14)= 4.991, p=0. }}–{56}} (t (14)=1.871, p=0.082) lossis 14–7–{66}} pawg (t(14 ). -2) pawg. Kev ntsuas kev ua tau zoo tsis cuam tshuam los ntawm qhov sib txawv ntawm lub sijhawm tshawb nrhiav khoom ntawm hnub xeem (Daim duab 1E; ib-txoj kev ANOVA: F (3,27)=2.264, p=0.1038). Qhov tseem ceeb, txhua pab pawg neeg tawm dag zog nyob rau hauv cov ntsiab lus kev sim, ntsuas los ntawm kev txo qis hauv kev mus ncig thiab kev ceev nrawm ntawm cov kev sib tham (Ntxiv Fig. S1 A–B; Tukey's post hoctest habituation day 1 vs 6, p < 0.05 rau 0–0–0 , 14–7–0 thiab 14–7–2 pawg; p=0.07 for14–0–0 pawg).
Thaum lub sijhawm tau txais kev sib ntsib, cov nas hauv txhua pab pawg tau nthuav tawm cov qhab nia qis thiab zoo sib xws ntawm kev ntxub ntxaug (DI) cov qhab nia, qhia tias tsis muaj kev nyiam rau ob qho kev kawm tsis txaus siab nyob rau hauv qhov chaw A1 thiab A2 (Daim duab 1B; ib-txoj kev ANOVA, DI: Pawg F ( 3,27) =2.604, p=0.0724). Zuag qhia tag nrho cov khoom tshawb nrhiav kuj zoo ib yam ntawm cov pab pawg (Daim duab 1C; ib-txoj kev ANOVA, DI: Pawg F (3,27)=1.243, p=0.3137), qhia tias cov khoom nyiam qhov sib txawv Hauv kev tshawb nrhiav tsis tuaj yeem ua rau pom qhov sib txawv hauv kev sim ua haujlwm.Ua ua ke, cov ntaub ntawv no qhia tau tias 14d ntawm qhov kev tawm dag zog thawj zaug txhim kho cov khoom-qhov chawmemory tsim nyob rau hauv subthreshold acquisition nyob rau hauv cov poj niam nas. Cov txiaj ntsig no tau khaws cia thoob plaws lub sijhawm qeeb qeeb thiab cov txiaj ntsig tuaj yeem rov ua haujlwm nrog 2dreactivating qoj ib ce.
3.2. Estrous theem cuam tshuam rau kev yeem lub log khiav haujlwm tab sis tsis ua haujlwm OLM
Lub voj voog estrous ntawm cov poj niam nas feem ntau spans 4-5 hnub, thaum lub sij hawm cev xeeb tub cov tshuaj hormones hloov pauv (Becker li al., 2017; Becker & Koob, 2016). Qhov tseeb, kev ua haujlwm ntawm lub log sib txawv thoob plaws lub voj voog estrous, qhia lub luag haujlwm ntawm cov tshuaj hormones circulatingsex hauv kev hloov kho lub cev ntawm cov poj niam nas (Novak li al., 2012; Sherwin, 1998). Yog li, peb sib piv kev khiav deb hla ntau theem ntawm estrouscycle los ntsuas lub luag haujlwm ntawm lub voj voog estrous ntawm lub log-khiav tus cwj pwm.
Hais txog kev kawm thiab kev nco, thaum qee qhov kev tshawb fawb qhia tsis muaj qhov cuam tshuam ntawm estrous theem ntawm kev coj cwj pwm kev ua haujlwm ntawm kev nco qab (Berry li al., 1997; Ter Horst et al., 2013; Keizer et al., 2017), lwm tus tau tawm tswv yim (Cordeira et al. al., 2018; Hokenson et al., 2021; Milad etal., 2009; Pompili et al., 2010; Tuscher et al., 2015; Warren & Juraska, 1997; Trask et al., 2020), suav nrog kev txhim kho kev ua tau zoo. OLM txoj hauj lwm thaum lub sij hawm proestrus thiab estrus theem (Frick & Berger-Sweeney, 2001; Frye li al., 2007; Paris & Frye, 2008; Pompili li al., 2010).
Li no, peb kuj sib piv OLM kev ua tau zoo ntawm cov theem estrous thaum lub sij hawm tau txais thiab cov hnub xeem kom txheeb xyuas cov kev cuam tshuam ntawm estrous voj voog ntawm OLM kev ua tau zoo nyob rau hauv pab pawg.Vim cov qauv me me, nas nyob rau hauv lub proestrus thiab estrus theem tau pab pawg andmice hauv metestrus thiab diestrus tau. muab tso rau hauv lwm pab pawg hauv kev tsom xam ntawm estrus onacquisition thiab retrieval. Hauv kev txheeb xyuas ntawm kev khiav deb, kev khiav txhua hnub tau teeb tsa nrog cov theem sib tsoo vim qhov qis qis qis thiab ntsuas nrog txhua theem sib cais thaum ntsuas qhov ntsuas qhov deb ntawm qhov pib 14- hnub.
Qhov cuam tshuam ntawm estrous ntawm kev khiav deb tsis tau pom nyob rau hauv kev ntsuam xyuas ntawm kev khiav txhua hnub (Fig. 2A; ob txoj kev ANOVA, Phase F (1,63)=0.10, p=0.748; Phase × Hnub F (13,424) =0.66, p=0.798), tab sis qhov cuam tshuam ntawm estrous theem tau pom thaum cov theem estrous tau sib cais thiab ntsuas qhov kev khiav deb (Daim duab 2B; ib-txoj kev ANOVA, Phase F(3,511)=2.88, p=0.035), qhov twg cov poj niam hauv estrus tau khiav ntau dua li poj niam hauv diestrus (Fig. Tukey's post hoc test, p=0 .034), tab sis tsis yog proestrus (p=0.133) los yog metestrus (p=0.949) theem.
Estrous theem ntawm kev cob qhia (Fig. 2C; ob-txoj kev ANOVA, PhaseF (1,23)=0.11, p=0.738; Phase × Exercise Group F (3,23) {{10 }}.70, p=0.559) lossis hnub xeem (Fig. 2D; ob-txoj kev ANOVA, Phase F (1,23)=1.79, p=0.193 ; Phase × Exercise Group F(3,23)=0.20, p=0.894) tsis cuam tshuam rau kev ua haujlwm. Ua ke, peb cov ntaub ntawv qhia tias nyob rau hauv txoj kab nrog rau lwm cov lus ceeb toom ntawm estrous thiab khiav deb, nas nyob rau hauv estrus ua rau ntau dua tag nrho kev khiav deb.
3.3. Kev tawm dag zog-txhim kho hippocampal LTP tau khaws cia thoob plaws qhov tsis muaj zog
Raws li tau pom saum toj no, peb cov kev tawm dag zog ua haujlwm pab txhawb OLM mus sij hawm ntev tom qab 14d ntawm kev tawm dag zog thiab 2d ntawm kev tawm dag zog rov ua haujlwm. Yog li ntawd, peb nug seb hippocampal synaptic plasticity puas tau txhim kho tom qab qoj ib ce kev kawm hauv tib tsiaj (Fig.1A). Cov kab mob hippocampal tau sau thiab ua tiav los ntsuas qhov kev ua haujlwm ntawm qhov chaw excitatorypostsynaptic muaj peev xwm (fEPSP) cov ntaub ntawv los ntawm stratum radiatum ntawm CA1b insponse rau stimulation ntawm Schaffer collateral-commissural projections hauv CA1c stratumradiatum.
Txhawm rau tshuaj xyuas cov kev hloov pauv hauv synaptic plasticity tom qab kawm tiav, peb tau siv ib lub tsheb ciav hlau ntawm 5 theta-burst stimulation (TBS) los ua kom muaj peev xwm mus ntev (LTP) hauv cov hlais uas tau sau tseg thaum lub sijhawm nco qhov rais, 1 teev tom qab OLM tau txais. Daim ntawv BDNFdependent no tau tshaj tawm yav dhau los los txhawb kom muaj zog muaj zog hauv nas (Acharya li al., 2019; Keizer li al., 2021; Kramár et al., 2004; Kwapis et al., 2018; Vogel-Ciernia et al. 2013; White et al., 2016). Nees nkaum feeb tom qab-TBS, fEPSPslope pib ruaj khov thaum LTP nkag mus rau theem sib koom ua ke, thaum lub sijhawm cov xwm txheej dynamicsynaptic tshwm sim los tswj lub zog synaptic mus sij hawm ntev (G. Lynch, 1998).
Hauv cov nplais npaj los ntawm txhua tus nas, TBS tau tsim cov peev txheej tam sim ntawd uas tau ploj mus thiab tom qab ntawd ruaj khov nyob rau hauv qab no 20 min (Fig. 3A). Stable LTP, ntsuas 50–60 minpost-TBS, tau pom nyob rau hauv kev ua si (Fig. 3B; ib-txoj kev ANOVA F (3,31) =9.32, p=0.0002) qhov twg tag nrho cov pab pawg uas tau txais kev tawm dag zog tau nthuav tawm qhov nce ntxiv ntawm kev muaj peev xwm cuam tshuam nrog kev tswj hwm sedentary (14–0–0: p=0.003, 14–7–0: p {{21} }.0002, 14–7–2: p=0.033), qhia txog kev txhim kho synaptic plasticity. Txhawm rau tshuaj xyuas seb qhov kev ua kom muaj zog no yog vim muaj kev hloov pauv hauv lub hauv paus neuronal muaj nuj nqi hauv hippocampus, peb tau tsim cov tswv yim / tso zis nkhaus thiab ntsuas kev hloov pauv hauv kev sib koom ua ke-mem tes.
Tsis muaj qhov sib txawv tseem ceeb ntawm cov pab pawg hauv txoj kab nqes ntawm txoj kab nkhaus rau fEPSP sloperesponses ntsig txog fiber ntau volley magnitude (Fig. 3C; ib-txoj kev ANOVA F (3,31)=0.35, p= 0. 79) los yog kev sib koom tes-pulse kev pab cuam (Daim duab. 3D; ob txoj kev ANOVA, pawg: F (3,31)=1.49, p =0.24, kev sib cuam tshuam: F (6,62) 1.70, p=0.13). Ua ke, cov ntaub ntawv no qhia tau hais tias ib qho kev tawm dag zog thawj zaug txhim kho hippocampal synaptic plasticity uas txuas ntxiv txawm tias tom qab kev tawm dag zog thiab tom qab kev tawm dag zog rov ua haujlwm yam tsis muaj kev hloov pauv hauv lub hauv paus neuronal zog. Nws yog ib qho tsim nyog sau cia tias qhov kev txhim kho ntev ntev ntawm hippocampal LTP yog qhov tsis sib haum nrog qhov kev ua tau zoo OLM tau pom tom qab kev ncua qeeb, uas peb hais hauv kev sib tham.
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