Kawm Txog Cov Txheej Txheem Flash Extraction Ntawm Echinaside Thiab Piloside Los Ntawm Cistanche Deserticola

Liu Yaowei 1, Su Zihao 2

1. Inner Mongolia Shibo Health Service Industry Investment Management Co., Ltd., Ordos 017000, Inner Mongolia; Southern Medical University, Guangzhou, Guangdong 510515

[Cov ntsiab lus]

Nyob rau hauv cov ntaub ntawv no, lub extraction txheej txheem ntawmEchinacosidethiabActeosidelos ntawmCistanche deserticolatau kawm. Cov txheej txheem rho tawm ntawm cov khoom nquag ntawm Cistanche deserticola tau ua kom zoo, thiab cov polysaccharide los ntawm Cistanche deserticola tau huv si; Lub cev thiab tshuaj lom neeg cov khoom ntawm ib tug refined polysaccharide los ntawm Cistanche deserticola, tsim cov nroj tsuag atlas ntawm Cistanche deserticola; Cov random amplified DNA polymorphism (RAPD) ntiv tes ntawm Cistanche deserticola tau qhia kom meej.Lub flash extraction txoj kev rau extractingEchinacosidethiabActeosidelos ntawm Cistanche deserticola yog qhov yooj yim, siab extraction tus nqi uas yog luv luv thiab siv sij hawm, thiab lub zog txuag, uas tuaj yeem muab lub hauv paus rau kev tsim cov tshuaj tshiab thiab kev tsim khoom ntawm echinacea thiab poolside, thiab muaj txiaj ntsig zoo dua.

Cov tshuaj tseem ceeb ntawm Cistanche deserticola-Phenylethanol glycoside

Cov tshuaj tseem ceeb ntawm Cistanche deserticola—Echinacoside

Cov tshuaj tseem ceeb ntawm Cistanche deserticola-Acteoside

[Cov lus tseem ceeb]Cistanche deserticola; Echinacoside; Acteoside; Flash extraction txheej txheem; Txoj kev kawm

[Suav Library Classification No.]qw 914[Cov ntaub ntawv qhia tus lej]A         [Txoj Cai No.]2096-5249 (2020) 23-0027-02

Cistanche deserticola ma, hu ua "desert ginseng",yog ib yam khoom uas muaj ib tug complex tshuaj muaj pes tsawg leeg, uas yog cais raws li ib tug multi-component complex system.Ntau yam ntawm nws cov khoom siv tshuaj muaj tsawg heev, thiab nws nyuaj rau khaws, tsim, lossis ua haujlwm rau qee qhov, uas yog lub hom phiaj ntawm cov neeg ua haujlwm tshawb fawb tam sim no ntawm Cistanche deserticola. Cov tshuaj Cistanche deserticola, lub luag haujlwm yog qhov tshwm sim ntawm kev sib koom ua ke ntawm ntau yam khoom, thiab txhua yam tshuaj tsis tuaj yeem tsis quav ntsej. Txawm li cas los xij, qhov ua tau zoo ntawm cov tshuaj no tseem yog lub ntsiab lus tsis meej, thiab qhov ua tau zoo ntawm kev ua haujlwm ntawm nws lub luag haujlwm tsis meej. Yog li ntawd, ntau cov kev tshawb fawb ntawm cov tshuaj no feem ntau yog ua los ntawm nws tag nrho los yog ib tug neeg cov tshuaj. Yog tias cov neeg ua haujlwm cuam tshuam xav tsim cov kev tshawb fawb yav tom ntej ntawm cov tshuaj suav tshuaj, lawv yuav tsum tau pib los ntawm kev xav ntawm kev tswj kom zoo thiab tsim thiab txhim kho cov txheej txheem kev tswj xyuas zoo ntawm cistanche deserticola niaj hnub no. Txoj kev ntsuam xyuas ntau tshaj plaws rau kev ruaj ntseg zoo ntawm Cistanche deserticola yog cov cuab yeej ntsuas ntiv tes, uas tuaj yeem muab cov ntaub ntawv ntxaws ntxaws los ntsuas qhov zoo ntawm cov khoom siv tshuaj los ntawm kev xav txog hom thiab ntau npaum li cas ntawm cov tshuaj lom neeg hauv Cistanche deserticola.

Cistanche extract hmoov

Nyem qhov no mus saib Cistanche Extract cov khoom

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1.Txoj kev sim

1.1 Xaiv txoj kev rho tawm ntawmCistanche deserticola

Tus kws sim ntsuas qhov hnyav 3 ntu qhuav thiab hmoov ntawm Cistanche deserticola 10.0g nrog qhov sib npaug. Ua ntej ntawm tag nrho cov, extract 100 g ntawm purified dej. Thawj feem ntawm cov hmoov cistanche deserticola yuav tsum tau muab rho tawm los ntawm txoj kev reflux. Hauv cov txheej txheem ua haujlwm tiag tiag, xyuas kom meej tias tus naj npawb ntawm reflux extraction yog 2 thiab txhua qhov kev rho tawm yog tswj ntawm li 2h.

Cov hmoov thib ob ntawm Cistanche deserticola yuav tsum tau muab rho tawm los ntawm txoj kev txias txias. Tom qab tshem tawm, cov neeg ua haujlwm cuam tshuam yuav tsum tau muab tso rau hauv qhov chaw sov, thaiv tag nrho lub teeb, thiab tsau nws tag nrho, thiab lub sijhawm soaking yog tswj ntawm 2 hnub [1].

Cov hmoov thib peb ntawm Cistanche deserticola yuav tsum tau muab rho tawm los ntawm txoj kev ultrasonic rau tus nqi sab hauv ntawm nws cov khoom siv tshuaj. Hauv kev ua haujlwm tiag tiag, kev rho tawm ultrasonic raug xaiv ob zaug, thiab lub sijhawm rho tawm ntawm txhua lub sijhawm yog tswj ntawm 1h. Tom qab ntawd, los ntawm kev ua haujlwm kom raug ntawm txoj kev txiav txim siab cov ntsiab lus polysaccharide, cov txheej txheem ua tiav peb feem hmoov Cistanche deserticola tau txheeb xyuas thiab muab piv rau tag nrho, thiab cov txiaj ntsig tau txheeb xyuas thiab muab piv. Txoj kev rho tawm tsim nyog tshaj plaws tau xaiv los ntawm ntau txoj kev rho tawm raws li qhov xav tau tiag tiag.

1.2 Kev soj ntsuam ntawm cov txheej txheem rho tawm

Thaum tshawb xyuas cov txheej txheem rho tawm tiag tiag, cov neeg ua haujlwm cuam tshuam yuav tsum tau ua kom zoo tshem tawm cov ziab qhuavSuav tshuaj ntsuab cistanche slices, thiab tom qab ntawd siv roj av ether ntawm 60 degree ~ 90 degree rau reflux extraction. Thaum lub sij hawm reflux extraction, nws yog ib qhov tsim nyog los xyuas kom meej tias lub ntim cov ntsiab lus ntawm roj av ether yog 10 npaug ntawm lub ntim ntawm qhuav npaj Cistanche deserticola slices. Thaum lub sij hawm reflux extraction, nws yuav tsum nruj me ntsis tswj qhov ntev ntawm reflux extraction, nyiam dua ntawm 2h / lub sij hawm. Tom qab refluxing extraction, cov neeg ua hauj lwm cuam tshuam yuav tsum tau lim tawm cov roj av ether, thiab ces qhuav cov npaj Cistanche deserticola slices, thiab ces refluxed lawv nrog ethanol. Thaum lub sij hawm refluxing extraction, nws yog ib qhov tsim nyog los xyuas kom meej tias lub ntim cov ntsiab lus ntawm ether yog 10 npaug ntawm lub ntim ntawm qhuav npaj Cistanche deserticola slices thiab hais tias cov ntsiab lus ntawm ether yog 80 feem pua. Thaum lub sij hawm reflux extraction, nws yuav tsum nruj me ntsis tswj lub sij hawm ntawm reflux extraction, nyiam dua ntawm 2h / lub sij hawm, rau tag nrho ntawm ob zaug. Tom qab reflux extraction, lim cov seemCistanche deserticola residue [2].

Cistanche deserticolahlais

Cov neeg ua haujlwm cuam tshuam yuav tshem tawm cov qhuavCistanche deserticola residuenrog distilled dej reflux. Tom qab reflux extraction, lub filtrate yuav muab ua ke rau kev ua, thiab ces nws yuav concentrated los ntawm teb evaporation. Tom qab ntawd, cov neeg ua haujlwm cuam tshuam yuav tsum tau teeb tsa lub siab ntawm ethanol ua qhov sib txawv thiab xaiv qhov sib txawv ntawm ether kom ntub cov lim dej qhuav. Thaum lub sij hawm soaking, nws yog ib qhov tsim nyog los xyuas kom meej tias lub ntim cov ntsiab lus ntawm ether yog 5 lub sij hawm lub ntim ntawm qhuav Cistanche deserticola, thiab sab hauv tsev kub ntawm ether thiab kub ntawm ether yog 4 degree. Tom qab sawv ntsug ib hmos, cov neeg ua haujlwm tsim nyog tsim lub centrifuge kom zoo khaws cov precipitated polysaccharides.Tom qab sau cov polysaccharides qhuav hauv lub tshuab nqus tsev, lawv dhau los ua cov polysaccharides ntawm Cistanche deserticola.

1.3 Kev txiav txim siab ntawm cov ntsiab lus polysaccharide

Cov ntsiab lus ntawm polysaccharides hauv Cistanche deserticola tau txiav txim siab los ntawm txoj kev phenol-sulfuric acid [3].

2 .Kev sim txoj kev los tsim cov kua theem ntiv tes ntawmCistanche deserticola

"Desert ginseng" Cistanche deserticola

2.1 Kev npaj cov tshuaj ntsuam xyuas

Cov neeg ua haujlwm cuam tshuam tau siv qhov sib npaug kom hnyav 1.0g qhuav thiab hmoov cistanche deserticola hlais. Ua ntej, ntxiv methanol rau 1. Tom qab ntawd siv ultrasonic extraction los rho tawm cov tshuaj. Lub sijhawm no, nws yog ib qho tsim nyog los tswj lub sijhawm ntawm kev rho tawm ultrasonic, uas yuav tsum tau tswj ntawm 30 feeb. Thaum lub sijhawm rho tawm, cov neeg ua haujlwm cuam tshuam yuav tsum siv cov tshuaj methanol hauv lub sijhawm los ntxiv qhov hnyav. Tom qab 30 feeb, cov neeg ua haujlwm cuam tshuam yuav noj 5mL ntawm cov lim lim thiab evaporate tag nrho. Txuas ntxiv ntxiv cov tshuaj methanol txaus rau hauv lub thawv, thiab tom qab ua tiav, hloov cov tshuaj mus rau 10mL volumetric flask. Cov neeg ua haujlwm cuam tshuam ntxiv cov tshuaj methanol kom txog rau thaum qhov kev daws qhov siab nce mus txog qhov ntsuas ntawm lub ntim ntim, thiab tsis txhob ntxiv cov tshuaj methanol. Cov neeg ua haujlwm cuam tshuam tau siv 0.45 µ m microporous membrane los lim cov tshuaj zoo, txuag cov tshuaj thiab npaj rau kev tos.

2.2 HPLC-UV chromatographic tej yam kev mob

Cov neeg ua haujlwm cuam tshuam tau sab laj nrog cov ntaub ntawv tseem ceeb thiab cov ntaub ntawv, thiab cov txiaj ntsig tau pom tias qhov kev sim tuaj yeem ua kom zoo dua qub, sib piv, thiab tshuaj xyuas los ntawm cov xwm txheej chromatographic, thiab kev sib xyaw zaum kawg tuaj yeem txhais tau zoo txog cov xwm txheej chromatographic: chromatographic kem Kromasil (250 × 4.6 mm, 5 µ m), tag nrho lub sij hawm tsom xam yog teem rau ntawm 90 min, txhaj tshuaj ntim yog 10 µ l, lub mobile theem yog 0.5 feem pua ​​​​acetic acid dej (B) thiab acetonitrile (A) gradient elution, tus nqi ntws yog tswj ntawm 1.0 mL / min, kev tshawb nrhiav wavelength yog 310 nm, thiab sab kub yog 28 degree [4].

2.3 Txoj kev tshawb nrhiav

Cov txiaj ntsig tau raug txheeb xyuas tau zoo los ntawm kev sim ua kom raug, kev sim rov ua dua, thiab kev sim ua kom ruaj khov.

3 .Cov ntsiab lus

Cistanche hmoov

Hauv daim ntawv no, kev sib xyaw ua ke ntawm ntau-kem chromatography tau siv los ua kom zoo sib cais thiab ua kom huv huv cov roj ntsha polysaccharide ntawm Cistanche deserticola. Nyob rau tib lub sijhawm, cov neeg ua haujlwm cuam tshuam kuj tau siv cov txheej txheem tshuaj ntsuam niaj hnub los txiav txim siab nws lub cev thiab tshuaj lom neeg.Cov txheej txheem sim thiab cov txiaj ntsig tau muab cov ntaub ntawv siv rau cov neeg ua haujlwm los kawm txog kev tswj xyuas zoo ntawm Cistanche deserticola. HPLC-DNA ntiv tes ntawm Cistanche deserticola tau tsim, thiab kev soj ntsuam zoo sib xws thiab kev tshuaj xyuas pawg tau ua los muab kev siv rau kev txheeb xyuas kev tshawb fawb thiab cov qauv zoo ntawm Cistanche deserticola.

siv

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[2] Wang Qian, Wang Qizhi, Shi Weijing, et al. Kev sib piv ntawm cov lus teb saum npoo txheej txheem thiab orthogonal tsim txoj hauv kev los txhim kho cov txheej txheem flash rho tawm ntawm tag nrho cov flavonoids los ntawm hollyhock [J]. Phau ntawv Journal ntawm Anhui University of Science thiab Technology, 2015,29 (05): 24-31

[3] Zhou K., Li X., Wang Z., et al. Optimization ntawm flash extraction txheej txheem ntawm asiaticoside thiab kev kawm sib piv ntawm txawv extraction txoj kev [J]. Suav Journal of Traditional Chinese Medicine, 2011,36 (23): 3265-3267

[4] Dong Jieqiong, Wang Yimin, Li Jianxia, ​​et al. Cov nyhuv ntawm cov tshuaj hormone induction thiab abiotic kev nyuaj siab rau cov ntsiab lus ntawm resveratrol nyob rau hauv lub hauv paus ntawm Polygonum cuspidatum thiab optimization ntawm lub flash extraction txheej txheem ntawm resveratrol nyob rau hauv lub hauv paus ntawm Polygonum cuspidatum [J]. Food Industry Science thiab Technology, 2016,37 (21): 210-215. Taw qhia rau tus sau: Liu Yaowei (1961.7 -), txiv neej, lub tuam tsev no, kev tshawb fawb kev taw qhia: kev noj qab haus huv loj thiab kev laus kev lag luam; Su Zihao (1996.8 -), txiv neej, kawm tiav, kev tshawb fawb kev taw qhia: biomedical engineering.