Kawm Txog Qhov Sib txawv ntawm Cistanche Tubulosa Inoculated Tubes Ntawm Qhov Sib Txawv Tamarix Inoculated Tubes

Abstract:Los ntawm kev kawm thiab sib piv cov kev loj hlob ntawm Tamarix thiabCistanche tubulosaThaum lub sij hawm thaum 9 hom ntawm Tamarix tau inoculated nrogCistanche tubulosa, cov txiaj ntsig tau pom tias: 7 ntawm 9 hom Tamarix qhia los ntawm psoma botanical vaj ntawm Xinjiang thiab Minqin counties tuaj yeem ua tiav inoculated nrog Cistanche tubulosa. Tus nab npawb ntawm parasiticCistanche tubulosaNyob rau tib lub sij hawm Tamarix raj paj nyob rau hauv lub xyoo, los ntawm siab mus rau qis, yog Tamarix alba, Tamarix ganmeng, Tamarix slender, Tamarix multi-branched, Tamarix densely flowered, Tamarix ntev-spike thiab Tamarix multi-flowered. Ntawm lawv, Tamarix multi-branched thiab Tamarix dawb-flowered Qhov siab tshaj plaws transverse diameters ntawm inoculated Cistanche tubulosa mus txog 4. 5cm thiab 4. 3cm raws li xyoo ntawd. Los ntawm kev soj ntsuam lub microstructure, nws tuaj yeem xaus lus tias piv nrog cov hauv paus hniav tamarix uas tsis muaj qhov sib txawv, cov hauv paus hniav ntawm Tamarix parasitized los ntawmCistanches tubulosayog thicker, thiab lawv cov phloem thiab xylem hlwb muaj ib tug loj npaum li cas ntawm enriched tshuaj (saccharides thiab lipids); 7 hom kab mob parasitic ntawm Cistanche tubulosa yog cov kab mob parasitism, uas lawv cuam tshuam cov hlwb los ntawm kev rhuav tshem cov cortical hlwb, thiab nws thiaj li txuas nrog cov ntaub so ntswg ntawm cov cell cell, nqus dej thiab cov as-ham los ntawm tus tswv tsev rau lawv tus kheej kev loj hlob thiab kev loj hlob xav tau.

Ntsiab lus:Cistanche tubulosa; kev nplua nuj; kab mob parasitism

CISTANCHE TUBULOSA

Cistanche tubulosayog ib tsob nroj herbaceous uas yog obligate perennial parasitic nroj tsuag, thiab nws tus tswv yogTamarix cov nroj tsuag[1]. Kev loj hlob ntawm Cistanche tubulosa noob muaj cov cai tshwj xeeb rau ib puag ncig. Yog tsis muaj pretreatment, germination tus nqi yuav tsawg heev. Yog li ntawd, ib tug germination-stimulating tshuaj yog xav tau los rhuav lub dormant lub xeev ntawm cov noob los txhawb lawv germination. Cov kev tshawb fawb pom tau hais tias ntxiv ib qho concentration ntawm GA3 ntxiv rau NAA ntxiv rau KT tov tshuaj thiab tswj qhov kub thiab txias ntawm li 23 degree tuaj yeem ua rau nce qhov germination ntawm Cistanche tubulosa noob [2, 3]. Fluridone daws ntawm 1 txog 100 mg·L-1 muaj kev txhawb nqa tseem ceeb ntawm Cistanche tubulosa noob [4]. Cistanche tubulosa tau kho nrog 10 mg·L-1 qhov hnyav ntawm fluridone ntxiv rau gibberellin rau 36 teev.

Cov noob germination tus nqi mus txog 60% [5].

Soak Cistanche tubulosa noob hauv dej distilled rau 24 teev, tom qab ntawd kho nrog 1 mg·L-1 gibberellin rau 72 teev thiab coj lawv hauv 100 mg·L-1 tshuaj fluridone ntawm 25 degree rau 21 hnub hauv qab. tsaus ntuj, uas tuaj yeem txhim kho kev loj hlob ntawm Cistanche tubulosa noob. tus nqi [6]. Qee cov kev tshawb fawb kuj tau pom tias cov tshuaj inducing rau germination ntawmCistanche tubulosanoob yog txo hydroquinones [7]. Yog li ntawd, cov hydroquinones muab rho tawm los ntawm cov hauv paus hniav ntawm Tamarix willow tuaj yeem siv los ua cov laj thawj los kawm txog lawv cov teebmeem ntawm kev loj hlob ntawm Cistanche tubulosa noob.

CISTANCHE TUBULOSA

Nyob rau hauv xyoo tas los no, kev tshawb fawb ntawm Tamarix inoculated nrog Cistanches tubulosa tau tsom mus rau kev kis tus kab mob ntawm cov tswv tsev thiab nws cov txheej txheem kev loj hlob, kev sib raug zoo ntawm Cistanche tubulose thiab tus tswv tsev Tamarix tubulosa, thiab kev kawm txog kev cog qoob loo.

Miao Zhongqin tau soj ntsuam cov txheej txheem kev loj hlob ntawm Cistanche tubulosa los ntawm kev kis kab mob mus rau cov noob los ntawm kev xa hluav taws xob hluav taws xob thiab kho qhov muag tsom iav thiab pom tias Cistanche tubulosa tsim haustoria ua ntej kev sib cuag nrog Tamarix cov hauv paus hniav, thiab haustoria invaded Tamarix cov hauv paus hniav los ntawm epidermis, cortex, phloem, thiab xylem. nyob rau hauv lem, thiab Cistanches tubulosa tau pom thawj zaug

Qhov tshwm sim ib theem zuj zus ntawm Cistanche tubulosa [8]; Ma Dongming et al. tau tsim ib qho hauv vitro inoculation system siv peeled Cistanche tubulosa noob thiab Tamarix willow [9], uas nteg ib lub hauv paus kev tshawb fawb rau kev kawm cov germination thiab parasitism txheej txheem ntawm Cistanche tubulosa noob. Chen Qiming tau kawm txog inoculation ntawm Tamarix tubulosa thiab Cistanche tubulosa loj hlob ib txwm muaj nyob rau hauv Moyu County, Xinjiang, thiab qhia tau hais tias ib tug ntau dua inoculation tus nqi nyob ze cov dej thiab cov saum npoo npog tuaj yeem txhim kho qhov ciaj sia taus ntawm Cistanche tubulosa [10]; Tu Pengfei et al. systematically elaborated nyob rau hauv lub dag afforestation technology ntawm Tamarix tubulosa. Kev cog qoob loo, sau qoob, ua, ntim, thiab khaws cov thev naus laus zis ntawm Cistanche tubulosa thiab Cistanche tubulosa muab kev siv rau cov kws tshaj lij thiab cov neeg ua liaj ua teb [11]; Wang Hongguo et al. tau tshawb fawb txog Cistanche tubulosa hauv tsev cog khoom cog cog hauv nroog Dongying, xeev Shandong.

Nws qhia tau hais tias qhov chaw cog qoob loo tuaj yeem txhawb txoj kev loj hlob ntawm Tamarix tamarisk thiab Cistanches tubulosa, nce tus nqi inoculation, thiab daws cov teeb meem ntawm cov kab mob rot thiab lub caij ntuj no Frost puas tsuaj los ntawm nag lossis daus ntau dhau [12]. Txawm li cas los xij, kev cog qoob loo tsis yog siv dav hauv kev tsim khoom, feem ntau vim tias cog qoob loo loj yuav nce nqi ntau lawm thiab txo cov txiaj ntsig; Nws raug pom zoo kom siv txoj kev no rau kev tshawb fawb txog kev tshawb fawb. Yang Taixin kawm 8 hom Tamarix qhia nyob rau hauv North Tuam Tshoj Plain thiab inoculated raj paj Cistanche tubulosa. Nws xaus lus tias muaj qhov sib txawv tseem ceeb hauv kev hloov pauv ntawm hom Tamarix sib txawv rau North China Plain, thiab tau tshuaj xyuas Tamarix Gansu thiab Tamarix Gansu raws li cov tswv tsev tsim nyog rau kev qhia hauv zos. Cultivation [13].

Hauv thaj chaw xws li yav qab teb Xinjiang, Alxa League hauv Inner Mongolia, thaj av saline-alkali ntawm North China Plain, thiab Yellow River Delta hauv xeev Shandong, thev naus laus zis ntawm Tamarix inoculated raj paj nrog Cistanche tubulosa tau maj mam loj hlob [{{1 }}]. Cov hom tseem ceeb ntawm Tamarix uas tau ua tiav inoculated muaj xws li Tamarix chinensis, Tamarix Gansu, Tamarix gaming, thiab Tamarix multibranch [19]. Txog rau tam sim no, vim muaj kev kub ntxhov, tsis muaj nag lossis daus kub, thiab kev puas tsuaj khov, thev naus laus zis ntawm inoculating Tamarix tube paj nrog Cistanche tubulosa tsis tau nthuav dav hauv cheeb tsam Hexi ntawm Gansu Xeev. Thaj chaw no yog nplua nuj nyob rau hauv tamarisk peev txheej. Yog tias cov peev txheej tamarisk tau tsim, tus nqi ntawm tamarisk nws tus kheej yuav nce siab heev. Txoj kev tshawb no coj 9 hom Tamarix qhia los ntawm Xinjiang thiab Minqin County thiab Cistanche tubulosa noob yuav los ntawm Xinjiang thiab Inner Mongolia ua cov khoom tshawb fawb. Los ntawm kev sib piv cov kev loj hlob ntawm Tamarix seedlings thiab Cistanche tubulosa thaum lub sij hawm inoculation ntawm 9 hom ntawm Tamarix, muab kev siv rau inoculating Tamarix raj paj Cistanche tubulosa nyob rau hauv cheeb tsam Hexi ntawm Gansu Province.

ACTIVE INGREDIENT NTAWM CISTANCHE

Nyem kom tau txais cov ntaub ntawv ntxiv

1 khoom

Cistanche tubulosa noobtau txais los ntawm Yutian County nyob rau yav qab teb Xinjiang Uygur Autonomous Region thiab Inner Mongolia Agricultural University. Cuaj hom tamarisk qhia los ntawm Xinjiang thiab Minqin County yog Tamarix elongata Ledeb., Tamarix hohenackeri Bunge, Tamarix androssowii Litw., thiab Tamarix ramosissima.

Lcdcb), Tamarix arceuthoides Bunge, Tamarix laxa Willd., Tamarix leptostachys Bunge, Tamarix hispida Willd., Tamarix austromongolica Nakai. Qhia 9 hom Tamarix uas loj hlob hauv cov suab puam saline-alkali av. Cov ntaub ntawv qhia yog cov ceg ntoo tshiab thiab cov ceg ntoo.

Qhov ntev yog 30cm thiab thickness yog 1. 0 ~ 1. 2cm. Txhua tsob ntoo tamarisk muaj 200 ceg. Los ntawm 2018 txog 2022, kev sim tshawb fawb txog kev txiav cov noob, cov ntaub so ntswg kab lis kev cai, thiab inoculation ntawm tube paj ntawm Cistanche tubulosa ntawm txawv Tamarix tau ua. Cov av ntawm kev sim teb yog xuab zeb.

2 txoj kev

2. 1 Kev soj ntsuam ntawm Cistanches tubulosa noob

Cov noob ntawm Cistanche tubulosa tau muab qhab nia thiab tshuaj xyuas siv {{0}}mesh, 35-mesh, 40-mesh, thiab 45-mesh sieves feem, thiab cov noob nrog txoj kab uas hla ntau dua ntau tshaj 0.5 mm raug xaiv rau qhov kev xeem no.

CISTANCHE TUBULOSA

2. 2 Pre-inoculation kev kho mob ntawm Cistanche tubulosa noob

Soak Cistanche tubulosa noob nyob rau hauv ib qho kev daws ntawm 1 mg·L-1 gibberellin thiab 10 mg·L-1 fluridone rau 30 mus rau 36 teev, ces lim tawm cov tshuaj tov ntau dhau nrog gauze thiab tso tseg.

2.3 Tamarix txiav substrate kho

Sib tov cov khoom noj khoom haus thiab cov xuab zeb huv ntawm qhov sib piv ntawm 1: 1 tusyees, thiab tua kab mob nrog 0.1% poov tshuaj permanganate ua ntej siv.

2.4 Inoculation ntawm Tamarix cuttings

Sib tov lub cistanches tubulosa ua ntej cov noob nyob rau hauv kauj ruam 2.2 nrog cov as-ham av 1:5 ua tiav hauv qib 2.3 thiab sib tov rau cov noob hnav khaub ncaws. Sprinkle cov noob sib npaug rau hauv cov pob tshab yas paj lauj kaub, thiab khaws cov noob kom deb ntawm lub paj. Qhov tob ntawm hauv qab yog hais txog 5cm, txoj kab uas hla ntawm lub paj lauj kaub yog 17cm, thiab ces 9 txawv tamarisk cuttings yog muab tso rau.

Soak nrog 200 mg·L-1 rooting hmoov rau 20 feeb thiab ces txiav cov cuttings rau hauv paj pots, 5 nroj tsuag ib lub lauj kaub. Thaum txiav, ceev faj kom qis kawg ntawm Tamarix cuttings nyob rau hauv nruab nrab ntawm inoculated Cistanche tubulosa noob los txhim kho txoj kev vam meej tom qab inoculation. Tus nqi. Tom qab suav txhua lub paj lauj kaub, muab tso rau hauv chav kab lis kev cai nrog qhov kub ntawm 23-25 degree, av av ntawm 30%, lub teeb / tsaus voj voog ntawm 12/12h, thiab lub teeb siv li ntawm 2000lx. Saib xyuas qhov kev txhaj tshuaj tiv thaiv tom qab 180 hnub.

CISTANCHE TUBULOSA

2.5 Kev npaj ntawm paraffin seem

Tamarix tamarisk thiab Cistanches tubulosa systems tau muab faib ua cov txheej txheem paraffin sectioning zoo tib yam, stained nrog safranin ceev ntsuab staining txoj kev, thiab luam theej duab nrog Panoramic 250FLASH (Hungary 3DHISTECH) scanner.