Kev Tshawb Fawb Txog Kev Tshawb Fawb Thiab Kev Ua Haujlwm Antioxidant Ntawm Polysaccharides Los Ntawm Cistanche Deserticola Deserticola

Abstract: Hauv daim ntawv no, cov suab thaj nruab nrab CLP1 thiab kua qaub qab zib CLP2 ntawm Cistanche deserticola tau los ntawm kev rho tawm cov polysaccharide los ntawm Cistanche deserticola deserticola thiab purifying cov crude polysaccharide CLP ntawm Cistanche deserticola deserticola nrog DEAE cellulose kem chromatography. Cov ntsiab lus ntawm tag nrho cov suab thaj, Uronic acid thiab protein nyob rau hauv Cistanche deserticola crude polysaccharide CLP, Cistanche deserticola nruab nrab qab zib CLP1 thiab Cistanche deserticola acid qab zib CLP2 tau txiav txim los ntawm ultraviolet spectrum scanning, thiab lub molecular hnyav thiab monosaccharide muaj pes tsawg leeg ntawm polysaccharide tau txiav txim. Nyob rau tib lub sijhawm, cov ntsiab lus ntawm Cistanche deserticola crude polysaccharide CLP Lub DPPH radical scavenging muaj peev xwm thiab tag nrho cov antioxidant muaj peev xwm ntawm nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola thiab acid qab zib CLP2 ntawm Cistanche deserticola deserticola tau soj ntsuam sim. Cov ntaub ntawv ntsuas tau pom tias muaj pes tsawg leeg ntawm crude polysaccharide CLP ntawm Cistanche deserticola deserticola, nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola, thiab kua qaub qab zib CLP2 ntawm Cistanche deserticola deserticola zoo ib yam, tab sis cov ntsiab lus txawv ho; Cov qab zib nruab nrab CLP1 ntawm Cistanche deserticola deserticola thiab cov kua qaub qab zib CLP2 ntawm Cistanche deserticola deserticola tuaj yeem tau txais ntau qhov kev sib tw thaum lawv tau sib cais thiab lim dej los ntawm DEAE cellulose chromatographic kem, thiab qhov hnyav molecular yog nyob nruab nrab ntawm 0 thiab 10000 Da, qhia. tias cov khoom tseem ceeb ntawm Cistanche deserticola deserticola polysaccharide yog cov suab thaj me me. Cov monosaccharides ntawm nruab nrab qab zib CLP1 nyob rau hauv Cistanche deserticola deserticola qhia tau hais tias cov monosaccharides muaj nyob rau hauv nruab nrab qab zib CLP1 nyob rau hauv Cistanche deserticola deserticola deserticola yog Mannose, Galactose uronic acid, qabzib, Galactose, thiab Arabinose; Kev txiav txim siab ntawm monosaccharides ntawm Cistanche deserticola acid qab zib CLP2 tau pom tias cov monosaccharides muaj nyob hauv Cistanche deserticola acid qab zib CLP2 feem ntau yog Rhamnose, Galactose uronic acid, qabzib, thiab Galactose. Qhov kev sim DPPH dawb radical scavenging thiab tag nrho cov antioxidant muaj peev xwm sim ntawm polysaccharides nyob rau hauv Cistanche deserticola pom tau hais tias cov antioxidant muaj peev xwm ntawm nruab nrab qab zib CLP1 nyob rau hauv Cistanche deserticola yog siab dua li ntawm crude polysaccharide CLP nyob rau hauv Cistanche deserticola thiab acid qab zib CLP2 nyob rau hauv Cistanche deserticola.

Cov lus tseem ceeb: Cistanche deserticola; Polysaccharides; Kev txheeb xyuas cov khoom; Antioxidant kev ua haujlwm; absorbance

Cistanche deserticola kev sim

Cistanche deserticola deserticola yog ib hom herbaceous parasitic nroj tsuag, uas muaj ntau ntawm phenolic glycosides, alkaloids, Phenethyl cawv glycosides, suab thaj, thiab cawv. Ntawm lawv, cov suab thaj feem ntau yog nyob rau hauv daim ntawv ntawm polysaccharides. Cistanche deserticola polysaccharides muaj zog tiv thaiv kab mob kev ua haujlwm [1-2]. Raws li qhov nruab nrab metabolite hauv tib neeg lub neej kev ua ub no, cov dawb radicals nyob rau hauv lub xeev tsis ruaj khov thiab muaj oxidation kev ua haujlwm siab, uas yuav ua rau muaj kev puas tsuaj rau cov ntaub so ntswg thiab cov kabmob ntawm biomacromolecules hauv tib neeg lub cev. Yog li ntawd, kev siv cov tshuaj antioxidants ntuj los tshem tawm cov dawb radicals tau dhau los ua cov lus qhia tseem ceeb ntawm kev tshawb fawb txog zaub mov tam sim no. Polysaccharide Cheebtsam hauv Cistanche deserticola muaj lub peev xwm antioxidant, uas tuaj yeem txhawb kev tshem tawm cov dawb radicals hauv tib neeg lub cev [3].

Cov txiaj ntsig ntawm cistanche tubulosa-Antioxidant

Hauv daim ntawv no, Cistanche deserticola deserticola tau raug coj los ua cov khoom tshawb fawb, thiab cov tshuaj polysaccharide tau muab rho tawm thiab cais tawm. Qhov hnyav molecular ntawm Cistanche deserticola deserticola polysaccharide tshuaj yog ntsuas los ntawm kev ua tau zoo Gel permeation chromatography. Nyob rau tib lub sijhawm, muaj pes tsawg leeg ntawm cov tshuaj polysaccharide tau txheeb xyuas qhov tob. Kev ua haujlwm antioxidant ntawm Cistanche deserticola deserticola deserticola polysaccharide tau soj ntsuam los ntawm kev ntsuas cov dawb radical scavenging muaj peev xwm thiab tag nrho cov antioxidant muaj peev xwm ntawm Cistanche deserticola deserticola polysaccharide Cheebtsam, Nws muab lub hauv paus theoretical rau txoj kev loj hlob thiab kev siv ntawm Cistanche deserticola deserticola thiab kev txhawb nqa tus nqi ntxiv.

Suab puam nyob cistanche

Nyem qhov no mus saib Cistanche cov khoom

【Nug ntxiv】 Email: cindy.xue@wecistanche.com / Whats App: 0086 18599088692 / Wechat: 18599088692

1 Kev npaj ua piv txwv ntawm Cistanche deserticola

Qhov hnyav 2.5 kg ntawm Cistanche deserticola, hlais nws, ntxiv 12.5L dej distilled, thiab boil rau 3h. Tom qab boiling, lim thiab sau lub filtrate. Ntxiv 75 feem pua ​​​​ethanol thiab cia nws sawv ntsug rau 24 teev. Centrifuge nws ntawm 4000r / min rau 30min, sau cov sediment, thiab khov thiab qhuav cov sediment kom tau crude polysaccharide CLP ntawm Cistanche deserticola [4]. Siv DEAE cellulose, tsau thiab o nws ua ntej degassing kev kho mob, thauj nws mus rau hauv DEAE cellulose anion pauv chromatography kem, thiab sib npaug nrog dej distilled thiab NaCl tov. Nyhav 100mg ntawm crude polysaccharide CLP los ntawm Cistanche deserticola deserticola thiab yaj nyob rau hauv 10 mL ntawm distilled dej. Tom qab ntxuav nrog dej distilled, sau cov eluant thiab mloog zoo rau nws kom khov-qhuav kom tau cov suab thaj nruab nrab CLP1 los ntawm Cistanche deserticola deserticola; Tom qab eluting nrog NaCl tov, cov eluent tau sau, concentrated, thiab khov-dried tom qab dialysis kom tau cov kua qaub qab zib CLP2 ntawm Cistanche deserticola [5].

Npaj cov suab thaj nruab nrab CLP1 thiab acidic qab zib CLP2 ntawm Cistanche deserticola rau hauv cov kev daws teeb meem polysaccharide nrog ib qho concentration ntawm 1mg / mL, feem. Siv lub 210-600nm spectrophotometer rau UV spectroscopy scanning. Thaum lub ncov tshwm ntawm 260-280nm, nws tau lees paub tias cov tshuaj muaj cov protein thiab nucleic acid [6].

Cov tshuaj tseem ceeb ntawm Cistanche deserticola

2 Kev txiav txim siab ntawm polysaccharide Cheebtsam ntawm Cistanche deserticola

Cov ntsiab lus ntawm tag nrho cov suab thaj hauv Cistanche deserticola tau txiav txim siab los ntawm txoj kev phenol sulfuric acid. Ntsuas {{0}}, 0.2, 0.4, 0.6, {{10}}}.8, thiab 1mL Cov kua qabzib nrog cov ntsiab lus ntawm 0.1mg / mL, thiab muab tso rau hauv cov raj iav. Ntxiv cov dej distilled mus rau qhov ntim tas li ntawm 1mL, thiab tom qab ntawd ntxiv 0.5mL phenol reagent nrog cov concentration ntawm 6 feem pua ​​​​thiab 2.5mL concentrated sulfuric acid rau hauv cov hlab ntsha. Co zoo thiab txias rau chav tsev kub. Siv lub spectrophotometer los ntsuas qhov nqus ntawm 490nm, nrog cov ntsiab lus qab zib raws li x-axis thiab absorbance raws li y-axis, kos tus qauv nkhaus, thiab xam qhov sib npaug regression [7-8].

Coomassie ci ntsa iab xiav txoj kev siv los txiav txim siab cov ntsiab lus ntawm cov protein hauv Cistanche deserticola cov qauv. Siv 1mL ntawm cov qauv qauv nrog ntau qhov sib txawv thiab cov qauv kev daws teeb meem nrog cov concentrations ntawm 0.1mg/mL, feem ntxiv 4mL ntawm Coomassie ci ntsa iab xiav tshuaj rau hauv txhua lub raj tshuaj, co zoo, thiab tom qab ntawd sawv ntsug. Siv lub spectrophotometer los ntsuas qhov nqus ntawm 495nm, thiab xam cov ntsiab lus protein hauv cov qauv raws li tus qauv nkhaus sib npaug [9].

Cov ntsiab lus ntawm Uronic acid hauv Cistanche deserticola tau txiav txim siab los ntawm txoj kev m-hydroxyphenyl. Noj 0.4mL ntawm cov qauv kev daws teeb meem nrog ntau qhov sib txawv thiab cov qauv kev daws teeb meem nrog qhov concentration ntawm 0.1mg / mL, ntxiv 2.5mL ntawm cov tshuaj sulfuric acid rau hauv lub raj kuaj, co nws kom zoo, thiab tom qab ntawd coj mus kuaj. ib 25 feeb boiling dej da dej, tshem tawm thiab txias rau hauv chav tsev kub, ntxiv m-hydroxyphenyl nrog ib tug concentration ntawm 0.3 feem pua ​​thiab sodium hydroxide tov nrog ib tug concentration ntawm 0.5 feem pua ​​rau hauv lub kuaj lub raj, co nws zoo, thiab tom qab ntawd muab tso rau 30min, Siv lub spectrophotometer los ntsuas qhov nqus ntawm 525nm, thiab xam cov ntsiab lus Uronic acid hauv cov qauv raws li tus qauv nkhaus sib npaug [10-11]. Qhov txheeb ze molecular hnyav ntawm Cistanche deserticola polysaccharide tau txiav txim siab los ntawm kev ua haujlwm siab gel chromatography, thiab cov monosaccharide muaj pes tsawg leeg ntawm Cistanche deserticola deserticola tau txiav txim siab los ntawm kev ua haujlwm siab ua kua chromatography [12].

3 Kev txiav txim siab thiab kev soj ntsuam ntawm polysaccharide Cheebtsam ntawm Cistanche deserticola

UV spectrum scanning nkhaus ntawm photometer ntawm 210-600nm yog qhia hauv daim duab 1

Daim duab 1 UV Spectral Scanning Curve (210-600nm)

Nws tuaj yeem pom los ntawm daim duab 1 tias qhov nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola muaj qhov pom tseeb qhov siab kawg ntawm 280nm, thiab cov kua qaub qab zib CLP2 ntawm Cistanche deserticola deserticola deserticola tsis muaj qhov pom tseeb nqus qhov siab ntawm 260-280nm, tab sis UV absorption spectrum yog siab. Txij li thaum Tryptophan thiab tyrosine muaj nyob rau hauv cov protein muaj txiaj ntsig zoo rau ultraviolet lub teeb ntawm 280nm, nws tuaj yeem txiav txim siab tias nruab nrab qab zib CLP1 ntawm Cistanche deserticola muaj glycoprotein, thiab nws tsis tuaj yeem txiav txim siab seb cov kua qaub qab zib CLP2 ntawm Cistanche deserticola muaj nucleic. acid thiab protein.

DEAE cellulose anion pauv chromatographic kem chromatography tuaj yeem adsorb ionic tshuaj thiab impurities ntawm chromatographic kem kom paub txog kev sib cais ntawm cov tshuaj polysaccharide. Tom qab sib xyaw thiab ziab cov dej distilled ntxuav, tag nrho ntawm 34.68 mg ntawm nruab nrab qab zib CLP1 tau los ntawm Cistanche deserticola, thiab tom qab eluting, concentrating, thiab ziab nrog NaCl tov, tag nrho ntawm 16.52 mg ntawm acid qab zib CLP2 tau los ntawm Cistanche. deserticola. Thaum lub sij hawm sim, tus qauv nkhaus sib npaug ntawm tag nrho cov ntsiab lus qab zib, cov ntsiab lus protein, thiab Uronic acid ntawm Cistanche deserticola tau txais. Cov ntsiab lus ntawm tag nrho cov suab thaj, protein, thiab Uronic acid hauv Cistanche deserticola tau ntsuas, raws li qhia hauv Table 1.

Table 1 Tag nrho cov suab thaj, protein, thiab Uronic acid cov ntsiab lus ntawm Cistanche deserticola

Qhov sib npaug regression tuaj yeem tau txais los ntawm kev txiav txim siab qhov hnyav molecular thiab qhov chaw siab kawg ntawm tus qauv qauv. Qhov hnyav molecular ntawm tus qauv yuav tsum tau kuaj tau los ntawm kev hloov lub photoemission teeb liab tus nqi ntawm cov qauv mus kuaj rau hauv qhov sib npaug regression. Kev soj ntsuam ntxiv thiab suav tuaj yeem tau txais tus lej nruab nrab molecular hnyav, qhov hnyav nruab nrab molecular hnyav, thiab dispersion coefficient ntawm nruab nrab qab zib CLP1 thiab acid qab zib CLP2 ntawm Cistanche deserticola. Lub sijhawm khaws cia thiab kev ntsuas qhov hnyav molecular ntawm qhov ua tau zoo ntawm cov gel permeation chromatogram ntawm Cistanche deserticola nruab nrab qab zib CLP1 thiab Cistanche deserticola acid qab zib CLP2 yog qhia nyob rau hauv Table 2. Tus naj npawb nruab nrab molecular hnyav yog siv los sawv cev qhov loj ntawm cov ntsuas molecule, thiab qhov piv ntawm qhov hnyav nruab nrab molecular hnyav rau tus lej nruab nrab molecular hnyav yog siv los sawv cev rau qhov dav molecular tis.

Table 2 Retention Peak Determination Time thiab Molecular Weight Calculation Results

Nws tuaj yeem pom los ntawm Table 2 tias qhov hnyav molecular faib ntau yam ntawm nruab nrab qab zib CLP1 ntawm Cistanche deserticola thiab cov kua qaub qab zib CLP2 ntawm Cistanche deserticola yog 0 ~ 10000 Da, uas qhia tias polysaccharide hauv Cistanche deserticola yog me me. molecule polysaccharide nrog sib txawv molecules, thiab qhov hnyav molecular qhia heterogeneity. Nws tuaj yeem suav tau tias cov suab thaj nruab nrab CLP1 ntawm Cistanche deserticola thiab cov kua qaub qab zib CLP2 ntawm Cistanche deserticola yog cov sib xyaw ntawm ntau cov tshuaj polysaccharide.

Saib Table 3 rau HPLC peaks thiab ncov lub sij hawm tuav ntawm cuaj monosaccharide qauv qauv, nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola thiab acid qab zib CLP2 ntawm Cistanche deserticola deserticola. Los ntawm kev sib piv lub sijhawm ncov, nws tuaj yeem txiav txim siab tias muaj tsib qhov pom tseeb monosaccharides hauv nruab nrab qab zib CLP1 ntawm Cistanche deserticola thiab plaub pom tseeb monosaccharides hauv cov kua qaub CLP2 ntawm Cistanche deserticola. Qhov kev faib ua feem ntawm monosaccharides nyob rau hauv nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola thiab acid qab zib CLP2 ntawm Cistanche deserticola deserticola yog qhia nyob rau hauv Table 4.

Table 3 Retention Time of Monosaccharide High-Performance Liquid Chromatography Peaks

Table 4 Feem ntawm Monosaccharides hauv Cistanche deserticola

Cov ntsiab lus monosaccharide ntawm nruab nrab qab zib CLP1 ntawm Cistanche deserticola tom qab hydrolysis yog qhov sib txawv ntawm cov kua qaub qab zib CLP2 ntawm Cistanche deserticola. Cov ntsiab lus qabzib ntawm cov suab thaj nruab nrab CLP1 ntawm Cistanche deserticola yog siab li 88.62 feem pua, cov piam thaj cov ntsiab lus ntawm cov kua qaub qab zib CLP2 ntawm Cistanche deserticola tsuas yog 9.99 feem pua, thiab Galactose aldehyde acid, Galactose thiab Rhamnose cov ntsiab lus ntawm cov kua qaub qab zib CLP2 ntawm Cistanche deserticola yog tag nrho ntau dua 5 feem pua.

4 Antioxidant kev tsom xam ntawm Cistanche deserticola polysaccharide

Thaum lub sij hawm kev soj ntsuam antioxidant ntawm Cistanche deserticola deserticola polysaccharide, Cistanche deserticola deserticola polysaccharide tau npaj rau hauv Cistanche deserticola deserticola polysaccharide tov nrog concentration ntawm {{0}}, 0.25, 0 .5, 1.0, 2. Thaum lub sij hawm kev txiav txim ntawm DPPH dawb radical scavenging tus nqi ntawm Cistanche deserticola deserticola polysaccharide tov, ntxiv 2 × Ib tug tag nrho ntawm 2mL ntawm 10-4mol / L DPPH yog tov tusyees thiab muab tso rau hauv maub tej yam kev mob rau 15 feeb. Cov tshuaj yog centrifuged ntawm centrifuge ceev ntawm 4000r / min thiab lub sij hawm centrifugation ntawm 15 feeb. Siv lub spectrophotometer los ntsuas qhov absorbance tus nqi ntawm cov tshuaj ntawm 517nm yoj, los ntawm qhov uas DPPH radical scavenging tus nqi ntawm Cistanche deserticola deserticola polysaccharide tov tau, raws li qhia nyob rau hauv Table 5.

Table 5 DPPH radical scavenging tus nqi ntawm Cistanche deserticola polysaccharide tov

Nws tuaj yeem pom los ntawm Table 5 tias qhov kev tshem tawm ntawm crude polysaccharide CLP ntawm Cistanche deserticola thiab nruab nrab qab zib CLP1 ntawm Cistanche deserticola ntawm DPPH dawb radicals yog ho ntau dua li cov kua qaub qab zib CLP2 ntawm Cistanche deserticola ntawm DPPH dawb radicals. Thaum cov concentration ntawm cov tshuaj yog tsawg dua 2.0mg / mL, DPPH radical scavenging tus nqi ntawm crude polysaccharide CLP ntawm Cistanche deserticola deserticola thiab nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola maj mam nce nrog nce concentration, thiab thaum kawg ua ruaj khov.

Thaum lub sij hawm txiav txim siab ntawm tag nrho cov antioxidant muaj peev xwm ntawm Cistanche deserticola deserticola polysaccharide, cov khoom siv tshuaj tua kab mob antioxidant tau siv rau kev tshawb pom sai. Siv cov tshuaj Trolox tus qauv kuaj pom thiab dilute nrog dej distilled rau {{0}}, 0.25, 0.5, 1.0, 2.{ {10}}, thiab 4.0 mg/mL, feem. Ntxiv cov tshuaj peroxidase ua hauj lwm rau qhov kev kuaj pom zoo, tom qab ntawd ntxiv cov qauv lossis cov qauv kev kuaj xyuas kom raug kuaj, thiab thaum kawg ntxiv cov kev daws teeb meem ntawm cov khoom siv tshawb nrhiav sai. Sib tov zoo thiab cia nws sawv ntsug rau 6 feeb, Tag nrho cov tshuaj tiv thaiv antioxidant ntawm Cistanche deserticola polysaccharide tau txiav txim siab los ntawm tus nyeem ntawv microplate ntawm lub wavelength ntawm 405nm thiab 734nm [14-15]. Saib Table 6 rau qhov kev xeem tau ntawm tag nrho cov tshuaj tiv thaiv antioxidant Achievement test ntawm Cistanche deserticola deserticola polysaccharide tov.

Table 6 Tag nrho cov antioxidant muaj peev xwm ntawm Cistanche deserticola polysaccharide tov

Nws tuaj yeem pom los ntawm Table 6 tias thaum qhov concentration ntawm Cistanche deserticola cov kua dej tsawg dua 4. CLP2 ntawm Cistanche deserticola nce nrog qhov nce ntawm cov tshuaj concentration; Lub peev xwm antioxidant ntawm nruab nrab qab zib CLP1 ntawm Cistanche deserticola deserticola yog qhov siab dua li ntawm crude polysaccharide CLP ntawm Cistanche deserticola deserticola deserticola thiab acid qab zib CLP2 ntawm Cistanche deserticola deserticola deserticola.

Cistanche tshuaj yej

5 Kev xaus

Cov txiaj ntsig kev soj ntsuam ntawm Cistanche deserticola deserticola polysaccharide los ntawm kev ua haujlwm siab Gel permeation chromatography qhia tau hais tias qhov hnyav molecular ntawm polysaccharide uas muaj nyob rau hauv nws tsis ntau tshaj 10000 Da, thiab nws muaj ntau yam elution peaks. Cistanche deserticola deserticola polysaccharide muaj qee yam antioxidant zog. Cov txiaj ntsig ntawm txoj kev tshawb no tau tsim lub hauv paus rau kev txhim kho ntxiv thiab kev siv ntawm Cistanche deserticola.

Cov ntaub ntawv:

 [1] Bi Cuicui, Liu Yinlu, Wei Fenfen, et al. Kev tshawb fawb tau nce qib ntawm cov tshuaj lom neeg tseem ceeb thiab cov dej num lom neeg ntawm Cistanche deserticola [J]. Kev Tshuaj Ntsuam Xyuas Tshuaj, 2019, 42 (9): 1896-1900

[2] Dong Yuwei, Miao Jingzhi, Li Yong. Optimization ntawm Ob Chav Enzyme Extraction Txheej Txheem rau Qej Polysaccharides thiab Nws yog nyob rau hauv Vitro Antioxidant Activity ［ J］. Suav Seasonings, 2020, 45 (10): 30-35, 48

[3] Yang Ting, Eladan McIntyre, Tao Yichun, et al. Kev txiav txim siab ntawm cov ntsiab lus polysaccharide thiab kev ua haujlwm antioxidant ntawm Cistanche tubulosa dregs ［ J］. Phau ntawv Journal ntawm Xinjiang Medical University, 2019, 42 (2): 234-236241

[4] Li Xu, Zhang Qing, Li Xu, et al. Kawm txog cov kuab tshuaj extraction tshuab ntawm Phenethyl cawv glycosides los ntawm Cistanche tubulosa [J]. Food Science thiab Technology, 2019, 44 (4): 225-229

[5] Liu Yaowei, Su Zihao. Kawm txog cov txheej txheem rho tawm flash ntawm echinacea thiab Verbascum thapsus glycosides hauv Cistanche deserticola ［ J］. Kev Noj Qab Haus Huv thiab Kev Noj Qab Haus Huv, 2020, 18 (23): 27-28

[6] Tian Wenjing, Zhou Xuan, Huang Qunhui, et al. Cov nyhuv ntawm kev kho ultrasonic ntawm Rheology zog ntawm Cistanche deserticola deserticola polysaccharide ［ J］. Phau Ntawv Tshaj Tawm ntawm Biology, 2021, 56 (2): 48-53

[7] Feng J, Guo Yehong, Zhu Wenjuan, et al. Kev cuam tshuam ntawm cov tswv tsev sib txawv, thaj chaw, thiab hnub sau qoob loo ntawm cov ntsiab lus ntawm Cov khoom xyaw nquag hauv Cistanche deserticola ［ J］. Suav Wild Plant Resources, 2021, 40 (5): 18-23

[8] Lv Minghuai, Zhao Bo, Sun Wenyu, et al. Kawm txog physicochemical zog thiab kev ua haujlwm antioxidant ntawm selenium-nplua nuj Auricularia auricula-judge polysaccharide ［ J］. Suav Flavoring, 2021, 46 (6): 54-59

[9] Chen Yueb. Kev tshuaj xyuas ntawm cov txheej txheem ntawm kev rho tawm Lentinan thiab kev soj ntsuam ntawm cov xwm txheej tam sim no ntawm kev tshawb fawb txog nws cov teebmeem kev noj qab haus huv [J]. Suav Flavoring, 2021, 46 (8): 189-191

[10] Guo Qiaoli, Wu Zhibo, Zhou Yubi, et al. Kev tshuaj ntsuam xyuas thiab kev ua haujlwm antioxidant ntawm polysaccharides los ntawm Cistanche deserticola hauv Lanzhou ［ J］. Food Industry Science thiab Technology, 2021, 42 (15): 96-103

[11] Cao Libo, Gong Xingcheng, Jia Jinru, et al. Kev soj ntsuam nrawm nrawm ntawm cov khoom siv tshuaj lom neeg ntawm Cistanche salsa los ntawm kev txhaj tshuaj ncaj qha rau ntau theem pawg spectrometry tag nrho scan txoj kev ［ J］. Suav Journal of Traditional Chinese Medicine, 2021, 46 (16): 4150-4156

[12] Hu Dongbao, Du Wei, Yang Meng. Kev ua kom zoo ntawm cov txheej txheem rho tawm thiab kev ua haujlwm antioxidant ntawm polysaccharide los ntawm Macrocybe gigantea los ntawm cov txheej txheem teb nto ［ J］. Suav Condiments, 2021, 46 (10): 78-82

[13] Xu Sheng, Chai Shasha. Txoj kev tshawb nrhiav sai ntawm cov khoom tseem ceeb hauv Cistanche deserticola [J]. Phau ntawv Journal of Wuhan Vocational and Technical College, 2021, 20 (4): 116-120

[14] Zhang HJ, Liu Xu, Jiao Li, et al. Kev ua kom zoo ntawm cov txheej txheem rho tawm ntawm Lentinan raws li quadratic regression rotation orthogonal method ［ J］. Suav Flavoring, 2021,46 (11): 88-93

[15] Song Qing, Zhang Ke, Li Ting, et al. Kev txiav txim siab ib txhij ntawm ntau cov ntsiab lus ntawm Cistanche deserticola los ntawm kev rov qab theem hydrophilic sib cuam tshuam chromatography customized multi-reaction xyuas txoj kev [J]. Kev tshuaj ntsuam chemistry, 2020, 48 (11): 1573-1593