Tseem ceeb Fade Of Neutralizing Antibodies Thiab Stable Cellular Immunity nyob rau hauv 4 Sijhawm COVID-19 Tshuaj Tiv Thaiv Tsis Muaj Tshuaj Tiv Thaiv Piv rau COVID-19 Convalescent Thiab 3 zaug txhaj tshuaj Hemodialysis Cov neeg mob
Jul 21, 2023
Hu rau Editor:
COVID-19 kev tawg tam sim no tshwm sim los ntawm Omicron variant ntawm kev txhawj xeeb (VOC) tau raug ntaus nqi los ntawm cov tshuaj tiv thaiv kab mob ploj tom qab SARSCoV-2 txhaj tshuaj tiv thaiv thiab ntau qhov kev hloov pauv hauv cov protein ntau ntawm Omicron VOC piv nrog spike protein ntawm cov tshuaj tiv thaiv tsiaj qus. Txawm li cas los xij, koob tshuaj txhaj tshuaj tiv thaiv kab mob uas ua rau muaj kev nce ntxiv hauv cov tsiaj qus-cov tshuaj tiv thaiv tshwj xeeb titer tuaj yeem them rau qhov txo qis rau tsawg kawg 3 lub hlis.1
Spike protein yog ib qho protein uas muaj nyob rau saum npoo ntawm cov kab mob, uas tuaj yeem pab cov kab mob khi rau cov kab mob thiab cuam tshuam cov hlwb, thiab yog ib qho tseem ceeb ntawm ntau cov kab mob los tua tib neeg.
Hais txog kev tiv thaiv kab mob, tib neeg lub cev tiv thaiv kab mob feem ntau yog nyob ntawm ob lub tswv yim hauv cov txheej txheem ntawm kev tawm tsam tus kab mob ntxeem tau, uas yog kev tiv thaiv kab mob ntawm tes thiab kev tiv thaiv kab mob humoral. Ntawm lawv, kev tiv thaiv kab mob ntawm tes tsuas yog tawm tsam cov kab mob los ntawm kev ua kom lub cev tiv thaiv kab mob (xws li T hlwb thiab cov cell killer ntuj); thaum humoral kev tiv thaiv tsuas yog nyob ntawm kev ua haujlwm ntawm cov tshuaj tiv thaiv kab mob txhawm rau txheeb xyuas thiab tshem tawm cov kab mob uas nkag mus rau lub cev.
Kev sib raug zoo ntawm spike protein thiab kev tiv thaiv kab mob tuaj yeem tshwm sim hauv cov hauv qab no:
Ua ntej ntawm tag nrho cov, spike protein yog ib qho tseem ceeb rau tus kab mob ntxeem tau, thiab nws lub neej ua rau tib neeg lub cev tiv thaiv kab mob ntsib teeb meem ntau dua. Yog li ntawd, kev tshawb fawb tshuaj tiv thaiv tsom rau cov kab mob sib kis tau dhau los ua ib qho ntawm cov lus qhia tseem ceeb hauv kev tawm tsam cov kab mob tam sim no. Kev txhaj tshuaj tiv thaiv tuaj yeem tso cai rau tib neeg lub cev tiv thaiv kab mob kom paub zoo dua thiab tua kab mob.
Nyem qhov txiaj ntsig kev noj qab haus huv ntawm cistanche
Qhov thib ob, cov kab mob sib kis yog cov qauv tseem ceeb ntawm tus kab mob, thiab nws cov antigenicity (uas yog, muaj peev xwm tsim cov tshuaj tiv thaiv kab mob nrog tib neeg lub cev tiv thaiv kab mob) muaj zog heev. Yog li ntawd, kev lees paub thiab nruab nrab ntawm cov kab mob sib kis los ntawm cov tshuaj tiv thaiv kab mob tau dhau los ua ib qho ntawm cov tswv yim tseem ceeb tiv thaiv tus kab mob. ib. Qee cov tshuaj tiv thaiv kab mob tiv thaiv kab mob tshwj xeeb, xws li cov tshuaj tiv thaiv kab mob monoclonal uas tam sim no tau siv dav hauv kev kho tus mob coronaviruses tshiab, yog tsim los ntawm lub hauv paus ntsiab lus no.
Thaum kawg, kev sib cuam tshuam ntawm cov protein ntau nrog lub cev tiv thaiv kab mob kuj hloov zuj zus. Qhov saum npoo ntawm cov protein ntau ntawm qee cov kab mob tshwm sim tuaj yeem hloov mus tas li, ua rau cov tshuaj tiv thaiv uas tsim los ntawm tib neeg lub cev tiv thaiv kab mob tsis paub txog nws, uas kuj yog ib qho nyuaj rau kev tshawb fawb ntawm qee cov tshuaj tiv thaiv kab mob. Yog li ntawd, hauv kev tshawb nrhiav thiab kev tshawb fawb tsis tu ncua, peb ntseeg tias peb tuaj yeem nrhiav txoj hauv kev zoo dua los tawm tsam cov protein ntau thiab txhim kho tib neeg kev tiv thaiv.
Raws li cov ntsiab lus, cov kab mob sib kis tau ua lub luag haujlwm tseem ceeb ntawm kev cuam tshuam tus kab mob thiab tib neeg lub cev tiv thaiv kab mob. Peb yuav tsum tau saib xyuas thiab kawm txog thaj chaw no kom tob kom tiv thaiv tib neeg kev noj qab haus huv zoo dua. Los ntawm qhov no peb tuaj yeem pom qhov tseem ceeb ntawm kev tiv thaiv kab mob, Cistanche tuaj yeem txhim kho kev tiv thaiv zoo vim tias cov khoom siv polysaccharide hauv Cistanche tuaj yeem tswj hwm kev tiv thaiv kab mob ntawm tib neeg lub cev, txhim kho kev ntxhov siab ntawm lub cev tiv thaiv kab mob, thiab txhim kho cov kab mob bactericidal ntawm lub cev tiv thaiv kab mob.
Yog li ntawd, cov boosters tsis tu ncua raug pom zoo tshwj xeeb tshaj yog rau cov neeg muaj kev pheej hmoo siab xws li cov neeg mob hemodialysis.
Peb txoj kev tshawb fawb yav dhau los hauv cov pej xeem sawv daws pom tau tias muaj kev lom zem thiab kev tiv thaiv kab mob ntawm tes tiv thaiv alpha, beta, thiab delta VOCs hauv cov neeg mob convalescent thiab txhaj tshuaj tiv thaiv piv rau cov neeg uas tau txhaj tshuaj thiab tsis muaj kab mob.2
Txoj kev tshawb no tsom mus tshawb txog kev lom zem thiab cellular qus-hom-tshwj xeeb thiab Omicron BA.4/5-kev tiv thaiv tshwj xeeb hauv cov neeg mob hemodialysis tom qab COVID-19 tshuaj tiv thaiv mRNA los ntawm kev sib piv cov tshuaj tiv thaiv COVID-19 tsis muaj kab mob (Vþ C) rau txhaj tshuaj tiv thaiv COVID-19 convalescent (Vþ Cþ) cov neeg mob hemodialysis.
Txhawm rau kom ntseeg tau tias txhua tus neeg mob muaj tib tus lej ntawm SARS-CoV-2 cov tshuaj tiv thaiv kab mob, 22 tus neeg mob uas tsis muaj kab mob hemodialysis uas tau txais 4 koob tshuaj tiv thaiv kab mob SARSCoV-2 mRNA (4 Vþ C) tau muab piv nrog 7 tus neeg mob nrog COVID{ {7}} tshwm sim los ntawm Omicron BA.1/2 uas tau txais 3 koob tshuaj tiv thaiv (3 Vþ Cþ) (Table Ntxiv S1).
Peb txheeb xyuas cov tshuaj tiv thaiv tsis zoo tiv thaiv SARS-CoV-2 wild-type and Omicron BA.4/5 VOC by SARS-CoV-2 spike protein (S protein) pseudovirus assays.
T-cell immunity reactive tiv thaiv SARS-CoV-2 wild-type and Omicron BA.4/5 overlapping peptide pools are analysised by multiparameter flow cytometry (Supplementary Methods and Supplementary Figure S1).
Hauv pawg tshuaj tiv thaiv nkaus xwb (4 Vþ C), 21 ntawm 22 tus neeg mob (95.5 feem pua) tau qhia txog kev tiv thaiv kab mob humoral tiv thaiv SARS-CoV-2 hom tsiaj qus, tab sis tsuas yog 8 ntawm 22 tus neeg mob (36.4 feem pua) tawm tsam Omicron BA.4/5 VOC. Nyob rau hauv sib piv, tag nrho 7 (100 feem pua) txhaj tshuaj tiv thaiv thiab convalescent (3 Vþ Cþ) cov neeg mob tau pom ib tug humoral tiv thaiv kab mob rau cov tsiaj qus thiab 6 ntawm 7 (85.7 feem pua) tawm tsam BA.4/5.
Txawm hais tias qhov tshwm sim ntawm cov neeg mob uas kuaj pom cov tsiaj qus-hom-reactive thiab BA.4/5-reactive CD4 thiab CD8 T hlwb zoo sib xws hauv ob pawg (Daim duab 1a), feem pua ntawm cov neeg mob uas muaj kev sib koom ua ke ntawm CD4 thiab cellular. thiab CD8 T-cell teb tau ntau dua hauv pawg tshuaj tiv thaiv kab mob convalescent (62.5 feem pua) piv nrog rau pawg tshuaj tiv thaiv nkaus xwb (25.0 feem pua) (Daim duab 1b).
Nyob rau hauv txoj kab nrog nws, titer ntawm neutralizing cov tshuaj tiv thaiv tiv thaiv Omicron BA.4/5 tau nce siab dua piv nrog cov neeg mob uas tau txhaj tshuaj nkaus xwb (Daim duab 1c).
Hauv kev sib piv rau kev tiv thaiv kev lom zem, tsis muaj qhov sib txawv ntawm feem pua ntawm cov tsiaj qus lossis Omicron BA.4/5- reactive CD4 thiab CD8 T hlwb thiab tsis muaj qhov sib txawv tseem ceeb hauv kev tsim cov proinflammatory cytokines interleukin -2 (IL -2) thiab qog necrosis factor (TNF) los ntawm cov hlwb ntawm 2 pawg neeg mob (Daim duab 1d–f).
Qhov xwm txheej siab ntawm cov neeg tsis txaus siab tsis txaus siab tawm tsam Omicron VOC tam sim no qhia qhov kev xav tau sai rau kev kho COVID-19 txhaj tshuaj tiv thaiv mRNA hauv cov neeg tsis muaj zog no.
Kev tiv thaiv kev tiv thaiv zoo dua qub uas pom hauv pawg neeg convalescent thiab txhaj tshuaj tiv thaiv raws li piv nrog cov tshuaj tiv thaiv-tsuas yog cov neeg mob tuaj yeem piav qhia los ntawm kev muaj zog antigenic stimuli, kev mob siab dua, kev txhawb nqa antigenic zoo dua, thiab kev siv lub cev tiv thaiv kab mob thaum lub sij hawm muaj kab mob zoo li piv nrog kev txhaj tshuaj.3
Qhia tawm
Txhua tus kws sau ntawv tshaj tawm tsis muaj kev sib tw txaus siab.
TXOJ CAI
Peb zoo siab ua tsaug rau cov neeg mob uas pub lawv cov ntshav kuaj thiab cov ntaub ntawv kho mob rau qhov project no.
Peb xav lees paub cov kws tshaj lij thiab kev pab cuam ntawm lub chaw kuaj kab mob tiv thaiv kab mob (Sviatlana Kaliszczyk, Patrizia Wehler, Eva Kohut, Julia Kurek) ntawm Lub Chaw Kho Mob Txhais Lus ntawm Marien Tsev Kho Mob Herne. Txoj haujlwm no tau txais kev txhawb nqa los ntawm cov nyiaj pab los ntawm Mercator Foundation thiab COVIDDataNet.NRW, thiab AIF/ZIM project EpiCov.
DAIM NTAWV THOV KEV PAB CUAM
Cov ntaub ntawv yuav muaj thaum thov.
SUPPLEMENTARY KHOOM
Cov txheej txheem ntxiv.
Daim duab S1.
Gating zoo.
Tab S1.
Kawm cov pej xeem.
Kev siv
1. Garcia-Beltran WF, St Denis KJ, Hoelzemer A, et al. mRNA-based COVID-19 cov tshuaj tiv thaiv kab mob ua rau muaj kev tiv thaiv tsis zoo tiv thaiv SARS-CoV-2 Omicron variant. Cell. 2022; 185:457–466.e4.
2. Paniskaki K, Konik MJ, Anft M, et al. Superior humoral immunity in vaccinated SARS-CoV-2 convalescence as piv rau SARS-COV-2 kab mob los yog tshuaj tiv thaiv. Pem hauv ntej Immunol.
3. Babel N, Hugo C, Westhoff TH. Kev txhaj tshuaj rau cov neeg mob lub raum tsis ua haujlwm: cov lus qhia los ntawm COVID-19. Nat Rev Nephrol. 2022; 18:708–723.
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