Phenylethanoid Glycosides Ntawm Cistanche Ntawm Menopausal Syndrome Model hauv nas
Mar 05, 2022
Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com
Shuo Tian, Mingsan Miao, Ming Bai, Zhenzhen Wei
Abstract
Cistancheyog cov tsoos suav tshuaj ntsuab thiab muaj txiaj ntsig zoo, nrog ob txhiab xyoo siv keeb kwm hauv Suav teb. Nws muaj cov nyhuv tonifying lub raum, muaj zog ntxiv rau lub siab thiab lub raum, thiab ntxiv cov ntsiab lus thiab cov ntshav, hu ua "suab puam ginseng". Ntawm no, peb tshawb xyuas cov txheej txheem ntawm Phenylethanoid Glycosides ntawmCistanche(PGC) rau cov nas qauv ntawm menopausal syndrome, nrog rau cov kev kho mob thiab cov yam ntxwv ntawm PGC rau menopausal syndrome. Hauv txoj kev tshawb no, KM nas tau rov tsim dua los ntawm kev ua tiav ntawm lub zes qe menyuam ntawm ob sab ntawm sab nraub qaum los tsim cov nas qauv ntawm tus mob menopausal syndrome (MPS), thiab tau txais cov dej distilled lossis tshuaj, raws li. Cov nas qauv tau txais cov dej distilled. Cov nas tau txais 200 mg / (kg hnub) siab ntau ntawm Phenylethanoid Glycosides ntawmCistanche(HPGC), thiab 100 mg / (kg hnub) nruab nrab koob tshuaj Phenylethanoid Glycosides ntawmCistanche(MPGC), thiab 50 mg / (kg ib hnub) qis dua ntawm Phenylethanoid Glycosides ntawmCistanche(LPGC). Tom qab 21 hnub, nws tuaj yeem txiav txim siab tus naj npawb ntawm cov haujlwm ywj pheej thiab tus naj npawb ntawm kev sawv, lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus, thiab tus lej hluav taws xob. Nws kuj suav cov viscera index ntawm lub tsev menyuam, thymus, spleen, ntsuas cov qib estradiol (E2), testosterone (T), luteinizing hormone (LH), thiab follicle-stimulating hormone (FSH) hauv cov ntshav. Tsis tas li ntawd, nws pom cov kev hloov pauv ntawm lub tsev menyuam, thymus, spleen thiab pituitary ntawm nas. Cov txiaj ntsig tau pom tias tus cwj pwm coj tus cwj pwm: Piv nrog rau pawg qauv (MG), HPGC, MPGC, LPGC tuaj yeem ua rau muaj kev ywj pheej ntau ntxiv (P < 0.01);="" hpgc,="" mpgc="" tuaj="" yeem="" nce="" tus="" naj="" npawb="" ntawm="" kev="" sawv,="" lub="" sijhawm="" latent="" ntawm="" thawj="" zaug="" nkag="" rau="" hauv="" chav="" tsaus,="" thiab="" txo="" tus="" lej="" hluav="" taws="" xob="" (p="">< 0.01);="" lpgc="" tuaj="" yeem="" nce="" tus="" naj="" npawb="" ntawm="" kev="" sawv="" (p="">< 0="" 05);="" viscera="" performance="" index:="" piv="" nrog="" mg,="" hpgc,="" mpgc="" tuaj="" yeem="" ua="" rau="" cov="" viscera="" performance="" index="" ntawm="" lub="" tsev="" menyuam,="" thymus,="" spleen="" (p="">< 0="" 01);="" lpgc="" tuaj="" yeem="" ua="" kom="" lub="" viscera="" performance="" index="" ntawm="" lub="" tsev="" menyuam="" (p=""><0.05); serum="" performance="" index:="" piv="" nrog="" mg,="" txhua="" pab="" pawg="" tuaj="" yeem="" txo="" qis="" lh="" hauv="" cov="" ntshav="" (p=""><0.01); hpgc,="" mpgc="" tuaj="" yeem="" txhim="" kho="" qib="" e2,="" t="" thiab="" txo="" qis="" lh,="" fsh="" hauv="" cov="" ntshav="" (p=""><0.01); lpgc="" tuaj="" yeem="" txhim="" kho="" qib="" e2="" thiab="" txo="" cov="" qib="" fsh="" hauv="" cov="" ntshav="" (p=""><0.05). lub="" caij="" no,="" nws="" muaj="" qhov="" sib="" txawv="" los="" txhim="" kho="" qib="" t="" hauv="" cov="" ntshav.="" pathological="" hloov="" pauv:="" piv="" nrog="" mg,="" hpgc="" tuaj="" yeem="" txhim="" kho="" cov="" kev="" hloov="" pauv="" pathological="" ntawm="" lub="" tsev="" menyuam,="" thymus,="" spleen="" thiab="" pituitary="" ntawm="" nas;="" lwm="" pab="" pawg="" kuj="" muaj="" tej="" yam="" tshwm="" sim.="" cov="" txiaj="" ntsig="" tau="" qhia="" tias="" pgc="" tuaj="" yeem="" txhim="" kho="" cov="" tshuaj="" hormones="" kev="" sib="" deev="" ntawm="" mps,="" thiab="" rov="" ua="" haujlwm="" ntawm="" lub="" tsev="" menyuam,="" thymus="" thiab="" spleen,="" nrog="" rau="" kev="" kho="" mob="" zoo="" dua="" ntawm="">
Taw qhia
Cistanche, lub qhuav fleshy stems nrog scaly nplooj ntawmCistanche deserticolaYC Ma and C.tubulosa(Schrenk) Wight (Chen et al., 2013), yog ib hom tshuaj nto moo thiab dav siv ntau txhiab xyoo. Nws yog keeb kwm los ntawm "Shen Nong's Herbal Classic" (Liu li al., 2013), ntsev hauv qab thiab sov hauv qhov xwm txheej. Nws muaj cov nyhuv tonifying lub raum, muaj zog ntxiv rau daim siab thiab lub raum, ntxiv cov ntsiab lus thiab cov ntshav, moistening cov hnyuv kom txo tau cem quav, thiab lwm yam kev ua tau zoo. Nws yog tsuas yog siv los kho lub raum-yang deficiency (ua rau impotence), prospermia, txias phev, thiab sterility vim txias nyob rau hauv lub tsev menyuam, cem quav, enuresis thiab zaus ntawm micturition, thiab lwm yam kab mob. Kev tshawb fawb niaj hnub no qhia tau hais tias nws tuaj yeem txhim kho tib neeg kev tiv thaiv kab mob, kev nco thiab kev kawm muaj peev xwm nrog kev tiv thaiv kev laus, tiv thaiv kab mob, tiv thaiv qaug zog, thiab lwm yam teebmeem.
MPS yog ib qho mob ntawm kev ua haujlwm ntawm lub paj hlwb autonomic dysfunction disorder los ntawm estrogen secretion disorder thiab nrog rau cov tsos mob neuropsychiatric. Nws muaj ntau theem ntawm kub flashes, chim siab, kiv taub hau, tinnitus, palpitations, insomnia, thiab lwm yam., nrog pom osteoporosis, nco poob, kev txawj ntse tsis zoo, kab mob plawv thiab cerebrovascular nyob rau hauv lub lig lub sij hawm. Qhov laj thawj tseem ceeb yog maj mam poob thiab ploj ntawm zes qe menyuam muaj nuj nqi (Ia, 2016). Tam sim no, qhov tseem ceeb ntawm kev siv tshuaj hormone hloov kho (HRT) rau kev kho mob MPS, cov tshuaj estrogen ncaj qha, HRT mus sij hawm ntev tuaj yeem ua rau qhov chaw mos los ntshav, mob lub mis, mob qog nqaij hlav endometrial, mob cancer mis thiab lwm yam teebmeem. Tsis tas li ntawd, cov nyhuv tseem tsis tau txaus siab - qhov koob tshuaj estrogen ntawm lub cev tiv thaiv kab mob tseem muaj kev cuam tshuam (Ma li al., 2016; Nawaz li al., 2017). Tshwj xeeb tshaj yog qhov kev tshawb pom tsis ntev los no qhia tau hais tias nws tuaj yeem ua rau muaj kev pheej hmoo ntawm cov kab mob plawv thiab paj hlwb thiab lwm yam kev mob tshwm sim loj heev uas txwv tsis pub siv tshuaj kho mob. Hauv kev xav tau ntawm kev lag luam sai, kev saib xyuas ntau dua los ntawm cov tshuaj suav tshuaj (TCM) los tshawb nrhiav kev kho mob MPS cov tshuaj (Halim thiab Phang, 2017; Mustafa et al., 2017). TCM kho MPS nrog keeb kwm ntev (Zhang thiab Miao, 2011), los ntawm kev tswj cov hypothalamic-pituitary-ovarian axis (HPOA), kev ua haujlwm ntawm zes qe menyuam, thiab kev laus ntawm zes qe menyuam qeeb. Nws tau pom tias feem ntau ntawm cov tshuaj ntawm lub raum yang muaj kev kho mob zoo dua ntawm MPS (Wei and Miao, 2013). Cistanche yog qhov ntau zaus ntawm cov tshuaj ntxiv rau lub raum yang rau yav dhau los dynasties (Tu li al., 2011). Lub ntsiab zoo ntawm Cistanche yog phenylethanoid glycosides, nrog rau cov nyhuv androgen. Nws yog lub embodiment ntawm niaj hnub tshuaj raum yang (Zhao thiab Pan, 2013), nrog lub siab tshaj plaws active cov khoom xyaw nyob rau hauv lub Cistanche (Yan li al., 2012). Nws yog feem ntau los ntawm ob txoj hauv kev ua haujlwm (Wumaierjiang thiab Yao, 2016). Ua ntej, nws muaj kev ua haujlwm ntxiv dag zog rau hypothalamus-pituitary-adrenal muaj nuj nqi, thiab txhawb kev tso tawm cov neurotransmitters thiab cov tshuaj hormones hauv lub cev; thib ob, nws muaj kev ua haujlwm ntawm kev qaug zog, txhim kho lub cev ua haujlwm. Cov nyhuv ntawm lub raum ntxiv ntawm Cistanche yog qhov sib txawv ntawm cov tshuaj raum dav dav xws li Epimedium brevicornum Maxim, Morinda Officinalis Yuav ua li cas. Cistanche tsis ua mob rau yin, tab sis ntxiv rau lub raum. Nws tsis tshwm sim nrog kev ruaj khov, qhov ncauj qhuav thiab lwm yam tsos mob rau kev siv mus ntev
2. Cov khoom siv thiab cov txheej txheem
2.1. Khoom siv thiab reagents
Cistanche(Batch No.20130501) tau yuav los ntawm Anhui, Dechang Pharmaceutical Pieces Co., Ltd. Cov qauv raug txheeb xyuas los ntawm xibfwb Chen Suiqing (lub Henan University of suav tshuaj, qhia txog Suav tshuaj qhuab qhia) raws li lub cev qhuav dej stems nrog scaly nplooj ntawmCistanche deserticolaYC Ma, nrog rau cov tawv nqaij qhuav nrog scaly nplooj ntawm C. tubulosa (Schrenk) Wight, feem. GC (Batch No.120303) yog muab los ntawm Shanxi hnub qub Pharmaceutical Co., Ltd.; Echinacoside siv tshuaj (Batch No.111670-200503) tau muab los ntawm National Institute for the Control of Pharmaceutical and Biological Products; AB -8 macroporous absorption resin (Batch No.20130618) yog muab los ntawm Tianjin Guangfu ntawm Lub Tsev Haujlwm ntawm Kev Lag Luam Tshuaj Superfifine; Sodium carboxymethyl cellulose (Batch No.20120418, Tianjin Hengxing Chemical Reagent Co., Ltd.), Benzylpenicillin Sodium rau Txhaj (Batch No.c1206807, North China Pharmaceutical Co., Ltd., tus specification: 4 lab units), 0.9% Chloride Txhaj Tshuaj (Batch No.1301265303, Chen Xin Pharmaceutical Co., Ltd; specifications: 250 ml), Chloral hydrate (Batch No.20120827, Tianjin Institute of Fine Chemical Industry), E2 ELISA assay kit (Batch No.201A, R. Tuam Tshoj), T ELISA assay kit (Batch No.20131001A, R&D Systems China), LH ELISA assay kit (Batch No. 20131001A, R&D Systems China), FSH ELISA assay kit (Batch No. 20131001A, R&D Systems China).
2.2. Kev npaj ua qauv
2.2.1. Txoj kev npaj
Cov txheej txheem rau kev npaj ua qauv yog raws li nram no: Los ntawm cov ntaub ntawv txoj kev (Gu li al., 2011), peb tau nyob rau hauv cov kev taw qhia ntawm Feng Suxiang (pharmaceutical tsom xam kev qhuab qhia, Henan University of suav tshuaj). CovCistanche yogcrushed rau pluas mov, los ntawm cov txheej txheem ntawm reflux extraction rau extract 2 zaug nrog tus nqi ntawm ethyl cawv (cov ntsiab lus ntawm ethyl cawv yog 70 feem pua). Lub sij hawm ntawm reflux extraction yog 1.5 h rau ib zaug thiab tom qab ntawd ua ke cov cawv extraction kua 2 zaug. Cov kua extraction tau decompressed thiab enriched yam tsis muaj cawv tsw, thiab cov distilled dej yog siv rau disperse (qhov concentration yog 0.5 g / ml, raws li cov qauv tov. Cov qauv tov yog ntsia rau hauv lub AB -8 resin nrog lub ntws (qhov piv ntawm cov qauv tov rau resin yog 1:10), tom qab sawv rau 5 h kom txog thaum cov qauv tov yog adsorbed tag nrho. }} resin nrog cov qauv tshuaj, nws tau tso tseg rau hauv dej; Thib ob, nrog 10 npaug ntawm sab ntim ntawm ethyl cawv (cov ntsiab lus ntawm ethyl cawv yog 10 feem pua), cov impurities raug tshem tawm; Thirdly, nrog 7 zaug ntawm kem ntim ntawm ethyl cawv (cov ntsiab lus ntawm ethyl cawv yog 60 feem pua) rau elution, peb sau cov eluate thiab qhuav lub eluate, uas yog cov hmoov ntawm Phenylethanoid Glycosides ntawmCistanche.
2.2.2. Kev xaiv ntawm kev txiav txim siab wavelength
Xaiv {{0}}.5 mL tswj cov khoom lag luam, thiab ntxiv 5 feem pua sodium nitrite tov 1 mL rau cov khoom tswj. Tom qab ntawd co thiab ntsiag to qhov chaw rau 6 min. Tom qab ntawd, ntxiv 10 feem pua ntawm txhuas nitrate tov rau saum toj no sib tov. Tom qab ntawd, co thiab ntsiag to qhov chaw rau 6 feeb. Ntxiv 10 feem pua sodium hydroxide 10 mL rau qhov sib tov saum toj no, qhov ntim ntawm qhov sib tov tau tsau rau 25 mL nrog dej. Co thiab nyob ntsiag to rau 18 min, raws li kev tswj cov khoom daws teeb meem. Xaiv 0.5 mL Phenylethanoid Glycosides ntawmCistanchekev daws, raws li cov txheej txheem saum toj no configuration, raws li kev xeem daws. Cov qauv dawb paug yog cov tshuaj dawb paug tshwj tsis yog rau kev tswj cov khoom lag luam thiab cov qauv kev daws teeb meem, zoo li cov qauv saum toj no teeb tsa. Nyob rau hauv lub UV spectrophotometer nrog lub wavelength ntau ntawm 200-800 nm, tag nrho wavelength yog siv los soj ntsuam cov saum toj no 3 kev daws teeb meem. Kev tswj cov khoom lag luam thiab cov qauv kev daws teeb meem muaj qhov siab tshaj plaws nqus ntawm 507 nm, yog li lub wavelength ntawm 507 nm tau txiav txim siab raws li qhov nqus wavelength. Tom qab Phenylethanoid Glycosides ntawmCistanchedaws (1) thiabEchinacosidecov khoom siv (2) (superposition contrast), xim tshwm (Saib daim duab tom ntej)
2.2.3. Txoj kev tshawb nrhiav cov ntsiab lus
Precise hnyav 1 mL tos rau qhov kev txiav txim ntawm kev daws, ntxiv 1 mL 5 feem pua sodium nitrite tov rau cov khoom tswj, co thiab nyob ntsiag to qhov chaw rau 6 min. Thiab tom qab ntawd ntxiv 10 feem pua aluminium nitrate tov rau saum toj no sib tov. Co thiab ntsiag to qhov chaw rau 6 min. Ntxiv 10 ml 10 feem pua sodium hydroxide rau saum toj no sib tov, thiab lub ntim ntawm cov sib tov yog tsau rau 25 mL nrog dej. Co thiab nyob ntsiag to rau 18 min; cov tshuaj dawb huv tau npaj los ntawm tib txoj kev raws li cov qauv dawb paug yam tsis muaj qauv tshuaj. Nrog rau UV spectrophotometric los txiav txim siab ntawm spectrophotometric ntawm 507 nm.
2.2.4. Txoj kev xeem
2.2.4.1. Kev npaj ntawm kev tswj kev daws teeb meem thiab cov qauv kev daws teeb meem.
Kev npaj ntawm kev tswj cov tshuaj: Precise hnyav 3. Tom qab ntawd siv 70 feem pua ethanol tov diluted rau lub teev, thiab co lub sib tov, raws li kev tswj kev daws. Cov ntsiab lus ntawm kev tswj cov tshuaj yog 0.1224 mg / ml. Kev npaj cov qauv kev daws teeb meem: Precise hnyav 5 mg Phenylethanoid Glycosides ntawm Cistanche thiab muab tso rau hauv lub khob ntsuas ntawm 10 mL. Tom qab ntawd siv 70 feem pua ethanol tov diluted rau lub teev, thiab co lub sib tov, raws li cov qauv tov.
2.2.4.2. Kev npaj ntawm tus qauv nkhaus.
Yog kos cov kev tswj kev daws teeb meem ntawm {{0}}, 1.0, 2.0, 3.0, 4.{{10 } }, 5. .296X 0.0015 (r=0.9994), thiab linear range yog 4.90–29.4 lg/ml.
2.2.4.3. Precision xeem.
Parallel txiav txim siab qhov nqus ntawm cov qauv tshuaj 6 zaug raws li cov khoom ntawm 'Txoj kev ntawm cov ntsiab lus tshawb pom". .299, 0.298, 0.297, 0.297, thiab 0.297, thiab qhov nruab nrab absorbance yog 0.298, nrog RSD=0.330 feem pua (n=6).
2.2.4.4 ib. Kev kuaj kev ruaj ntseg.
Txiav txim siab qhov nqus ntawm cov qauv tshuaj tom qab cov tshuaj muaj xim tag nrho rau {{0}}, 15, 30, 50, thiab 60 min raws li cov khoom ntawm "Txoj kev tshawb nrhiav cov ntsiab lus". {15}}.362 feem pua (n=5).
2.2.4.5. Kev sim rov ua dua.
Npaj 6 cov qauv kev daws teeb meem raws li cov khoom ntawm Kev Npaj ntawm cov qauv kev daws teeb meem", thiab suav cov absorbance thiab cov ntsiab lus raws li cov khoom ntawm "Txoj kev ntawm kev tshawb nrhiav cov ntsiab lus". Cov ntsiab lus yog 65.2 feem pua, 65.6 feem pua, 66.7 feem pua, 67.2 feem pua, thiab 66.5 feem pua, thiab cov ntsiab lus nruab nrab yog 66.2 feem pua, nrog RSD=1.142 feem pua (n=6).
3. Cov txiaj ntsig
3.1. Cov txiaj ntsig ntawm kev ua haujlwm ywj pheej thiab tus naj npawb ntawm kev sawv hauv nas
Fig. 1 thiab 2 qhia cov ntaub ntawv ntawm cov haujlwm ywj pheej thiab tus naj npawb ntawm kev sawv. Nws qhia tau hais tias MG nas tau txo qis heev piv nrog BC (P < 0="" 01),="" qhia="" txog="" qhov="" xav="" paub="" txog="" qhov="" chaw="" tshiab="" yog="" txo.="" cov="" nas="" nyob="" hauv="" gc,="" hpgc,="" mpgc,="" thiab="" lpgc="" tuaj="" yeem="" txhim="" kho="" cov="" haujlwm="" ywj="" pheej="" zoo="" kawg="" li="" piv="" nrog="" mg="" (p="">< 0.01);="" cov="" hauv="" gc,="" hpgc="" thiab="" mpgc="" tuaj="" yeem="" txhim="" kho="" cov="" haujlwm="" ywj="" pheej="" piv="" nrog="" mg="" (p=""><0.01); cov="" nyob="" hauv="" lpgc="" tuaj="" yeem="" txhim="" kho="" tus="" naj="" npawb="" ntawm="" kev="" sawv="" me="" ntsis="" piv="" nrog="" mg="" (p=""><>
3.2. Cov nyhuv ntawm lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus ntuj thiab tus lej hluav taws xob
Raws li pom hauv Fig. 3 thiab 4, cov ntaub ntawv ntawm lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus ntuj thiab tus lej hluav taws xob tau pom tias MG nas tau txo qis heev piv nrog BC (P <>
qhia txog qhov poob ntawm kev nco hauv MPS nas. Cov nas hauv GC, HPGC, thiab MPGC tuaj yeem ua rau lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus thiab txo tus lej hluav taws xob piv nrog MG (P < 0.01),="" nrog="" rau="" txhim="" kho="" lub="" cim="">
3.3. Cov nyhuv ntawm viscera index
Raws li pom nyob rau hauv daim duab 5, cov ntaub ntawv ntawm lub viscera Performance index ntawm lub tsev menyuam, thymus thiab spleen qhia tau hais tias MG nas yog remarkably txo qis piv nrog rau cov BC (P < 0.01),="" qhia="" txog="" cov="" uterus="" thiab="" lub="" cev="" tiv="" thaiv="" kab="" mob="" ntawm="" mps="" nas="" yog="" atrophied.="" cov="" nas="" nyob="" hauv="" gc,="" hpgc,="" thiab="" mpgc="" tuaj="" yeem="" txhim="" kho="" qhov="" viscera="" performance="" index="" ntawm="" lub="" tsev="" menyuam,="" thymus,="" thiab="" spleen="" piv="" nrog="" mg="" (p="">< 0.01).="" cov="" nas="" nyob="" rau="" hauv="" lpgc="" yog="" me="" ntsis="" txhim="" kho="" qhov="" viscera="" performance="" index="" ntawm="" lub="" tsev="" menyuam="" piv="" nrog="" mg="" (p=""><>
3.4. Cov ntsiab lus ntawm E2, T, LH, thiab FSH
Raws li pom nyob rau hauv daim duab 6, cov ntaub ntawv ntawm qib E2, thiab T qhia tau hais tias MG nas yog remarkably txo, thiab cov ntaub ntawv ntawm cov theem ntawm LH, thiab FSH qhia tau hais tias MG nas yog remarkably nce piv nrog cov BC (P < 0.01),="" nws="" qhia="" tau="" hais="" tias="" cov="" tshuaj="" hormones="" poj="" niam="" txiv="" neej="" nyob="" rau="" hauv="" cov="" ntshav="" tsis="" zoo="" nyob="" rau="" hauv="" mps="" nas.="" txhua="" pawg="" ntawm="" nas="" tau="" poob="" qis="" nrog="" qib="" lh="" hauv="" cov="" ntshav="" qab="" zib="" piv="" nrog="" bc="" (p="">< 0="" 01).="" cov="" nas="" hauv="" gc,="" hpgc,="" thiab="" mpgc="" tuaj="" yeem="" txhim="" kho="" e2,="" t="" thiab="" txo="" qis="" lh,="" fsh="" hauv="" cov="" ntshav="" piv="" nrog="" mg="" (p=""><0.01). cov="" nas="" hauv="" lpgc="" tuaj="" yeem="" txhim="" kho="" e2="" me="" ntsis="" thiab="" txo="" qis="" fsh="" hauv="" cov="" ntshav="" piv="" nrog="" mg="" (p=""><>
3.5. Kev soj ntsuam ntawm pathological morphological hloov ntawm lub tsev menyuam
Raws li pom hauv Fig. 7 thiab 8, nrog rau qhov ntsuas micrometer, qhov kev hloov pauv ntawm lub tsev menyuam qhia tau tias MG nas muaj cov kab mob tseem ceeb hauv lub tsev menyuam piv nrog BC (P < 0.01).="" cov="" nas="" nyob="" hauv="" gc,="" hpgc="" tuaj="" yeem="" txhim="" kho="" qhov="" kev="" hloov="" pauv="" ntawm="" cov="" kab="" mob="" pathological="" piv="" nrog="" mg="" (p="">< 0.01).="" cov="" nas="" hauv="" mpgc="" tuaj="" yeem="" txhim="" kho="" cov="" kev="" hloov="" pauv="" ntawm="" cov="" kab="" mob="" pathological="" piv="" nrog="" mg="" (p=""><0.05). cov="" kev="" hloov="" pauv="" ntawm="" cov="" kab="" mob="" morphological="" tau="" tshaj="" tawm="" raws="" li="" cov="" txheej="" txheem="" ib="" nrab="" los="" ntsuas="" lub="" tsev="" menyuam,="" thiab="" lub="" koom="" haum="" pathological="" morphology="" tuaj="" yeem="" muab="" faib="" ua="" plaub="" theem="" rau="" txhua="" pawg="" thiab="" suav="" cov="">
3.6. Kev ntsuas lub thickness ntawm thymic cortex
Raws li pom hauv Fig. 9 thiab 10, nrog rau qhov ntsuas micrometer, hnub tim ntawm lub thickness ntawm lub thymic cortex qhia tau hais tias MG nas raug txo qis dua piv nrog BC (P < 0.01),="" qhia="" tias="" qhov="" ntim="" ntawm="" thymus="" yog="" txo="" qis="" tom="" qab="" tsim="" mps="" qauv="" nas.="" cov="" nas="" nyob="" rau="" hauv="" hpgc="" tuaj="" yeem="" ua="" rau="" kom="" cov="" tuab="" ntawm="" thymic="" cortex="" piv="" nrog="" mg="" (p=""><0.01).>nas nyob rau hauv MPGC tuaj yeem nce qhov tuab ntawm thymic cortex com pared nrog MG (P < 0.05).
3.7. Ntsuas lub thickness ntawm splenic nodule
Raws li pom hauv Fig. 11 thiab 12, nrog rau qhov ntsuas micrometer, hnub tim ntawm lub thickness ntawm splenic nodule qhia tau hais tias MG nas raug txo qis dua piv nrog BC (P < 0.01),="" qhia="" tias="" qhov="" ntim="" ntawm="" tus="" spleen="" yuav="" txo="" qis="" tom="" qab="" tsim="" mps="" qauv="" nas.="" cov="" nas="" nyob="" rau="" hauv="" hpgc="" tuaj="" yeem="" ua="" rau="" kom="" cov="" tuab="" ntawm="" cov="" splenic="" nodule="" piv="" nrog="" mg="" (p="">< 0.01).="" cov="" nas="" nyob="" rau="" hauv="" mpgc="" tuaj="" yeem="" ua="" kom="" cov="" tuab="" ntawm="" cov="" splenic="" nodule="" piv="" nrog="" mg="" (p=""><>
3.8. Xam cov naj npawb ntawm cov basophilic cell thiab anterior pituitary cell
Raws li pom hauv Fig. 13 thiab 14, hnub tim tus naj npawb ntawm basophilic cell thiab anterior pituitary cell qhia tias MG nas raug txo qis dua piv nrog BC (P < 0.01),="" qhia="" tias="" tus="" lej="" ntawm="" lub="" cell="" ntawm="" secretory="" gonadotropin="" txo="" qis="" tom="" qab="" tsim="" mps="" qauv="" nas.="" tag="" nrho="" cov="" pab="" pawg="" tau="" txais="" cov="" tshuaj="" tuaj="" yeem="" ua="" rau="" muaj="" tus="" lej="" ntawm="" basophilic="" cell="" piv="" nrog="" mg="" (p="">< 0.01).="" cov="" nas="" nyob="" rau="" hauv="" gc,="" hpgc,="" thiab="" mpgc="" tuaj="" yeem="" ua="" rau="" muaj="" tus="" naj="" npawb="" ntawm="" cov="" pituitary="" anterior="" cell="" piv="" nrog="" mg="" (p=""><>
4. Kev sib tham
Cov ntaub ntawv ntawm tus mob menopausal nyob rau hauv cov phau ntawv thaum ub feem ntau yog tawg nyob rau hauv cov lus piav qhia ntawm "lily kab mob", "zangzao" syndrome, "melancholia", "kev coj khaub ncaws txawv txawv" thiab lwm yam (Chen li al., 2015; Ma thiab Chen, 2015) . Rau tus poj niam tshaj plaub caug xyoo, Yin Qi raug txiav ib nrab; Tiangui tau qhuav lawm; lub raum Qi tau maj mam poob; Yin Jing tsis muaj peev xwm; qhov nyiaj tshuav ntawm Yin thiab Yang tau poob lawm. Nws qhov kev ua tau zoo tseem ceeb yog raws li kev coj khaub ncaws los yog kev ua cev tsis muaj zog tshwm sim xws li kub taub hau thiab tawm hws, kev ntxhov siab, tsis xis nyob, palpitation thiab insomnia, mob nraub qaum, edema ntawm lub ntsej muag thiab ceg ceg, kiv taub hau, tinnitus, thiab tawv nqaij zoo li kab mob nkag mus. Lub zog poob ntawm lub raum Qi yog qhov laj thawj ntawm kev coj khaub ncaws tuaj lossis txiav tawm. Lub raum tsis muaj peev xwm yog lub hauv paus yog vim li cas ntawm menopausal syndrome (Tan li al., 2013), thiab lub raum yog lub hauv paus ntawm tib neeg lub neej, hu ua "congenital foundation". Qhov nce thiab poob ntawm lub raum essence dominate txoj kev loj hlob thiab kev ua me nyuam ntawm kev loj hlob thiab poob (Wang and Huang, 2011). Cov kev tshawb fawb tau pom tias kev poob qis ntawm zes qe menyuam ua haujlwm yog qhov tseem ceeb ntawm lub raum tsis muaj zog, thiab qhov tsis txaus ntawm Yin thiab Yang ntawm lub raum muaj feem cuam tshuam nrog kev cuam tshuam ntawm endocrine secretion thiab autonomic nervous system dysfunction. Kev hloov ntawm E2, FSH, thiab LH yog ib qho kev qhia ntawm lub raum tsis muaj peev xwm thiab lub raum Yin thiab Yang imbalance (Shi et al., 2007). Tonifying raum yang tshuaj yog siv los tswj cov tshuaj hormones nyob rau hauv lub hypothalamus-pituitary zes qe menyuam axis los yog ua lub luag hauj lwm ncaj qha nyob rau hauv regulating zes qe menyuam muaj nuj nqi. Cov tshuaj suav niaj hnub ntseeg hais tias etiology thiab pathogenesis ntawm menopausal syndrome yog raum tsis muaj zog (Razali thiab Said, 2017). Txoj kev tshawb no tau siv qhov kev txiav tawm tag nrho ntawm zes qe menyuam ntawm ob sab ntawm sab nraub qaum los tsim cov qauv MPS nas. 90 feem pua ntawm cov poj niam estrogen tau zais los ntawm zes qe menyuam, thiab lub zes qe menyuam raug tshem tawm. Nws ua rau cov tshuaj estrogen txo qis tam sim ntawd thiab tom qab ntawd simulate MPS. Cov qauv no yog cov qauv classic, nrog kev ua tiav siab, ruaj khov thiab kev ntseeg tau thiab lwm yam. Los ntawm kev ua haujlwm ywj pheej thiab tus naj npawb ntawm kev sawv, lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus ntuj thiab tus lej hluav taws xob, peb tau soj ntsuam kev txhim kho ntawm kev kawm thiab kev nco ua haujlwm thiab kev xav paub ntawm qhov chaw tshiab los ntawm cov tshuaj. Lub zes qe menyuam ua haujlwm tau poob qis nrog kev sib deev hormone secretion tsawg. Tsis tas li ntawd, gonadotropin tau nce nrog MPS cov neeg mob. Cov theem ntawm E2, T hauv cov ntshav tau qis dua tus neeg ntawd yam tsis muaj MPS cov tsos mob, thaum theem ntawm LH, FSH tau siab dua tus neeg ntawd tsis muaj cov tsos mob MPS. Yog li ntawd, los ntawm kev ntsuas ntawm E2, T, FSH thiab LH qib hauv cov ntshav, nws tuaj yeem siv cov ntsuas pom pom rau kev txheeb xyuas MPS, muaj kev cuam tshuam cov tshuaj ntawm MPS tus kab mob. Txoj kev tshawb no qhia tias kev ua haujlwm ntawm zes qe menyuam poob yog qhov ua rau lub raum tsis txaus, thiab kev hloov ntawm E2, T, FSH thiab LH yog ib hom kev qhia ntawm lub raum tsis txaus (Li et al., 2014; Cheng and Tian, 2012). Cistanche yog los ntawm cov txiaj ntsig ntawm kev ntxiv rau lub raum txhawm rau txhim kho kev tiv thaiv kab mob (Zhang li al., 2009; Shareef li al., 2017). Peb siv cov tshuaj ntxiv rau lub raum los kho MPS, uas yog qhov tshiab. Cov txiaj ntsig kuj tau pom tias PGC tuaj yeem txhim kho qhov tsis sib haum xeeb ntawm MPS nas, thiab nce qib E2, T, LH, thiab FSH hauv cov ntshav. Lub caij no, nws tuaj yeem txhim kho cov kev hloov pauv ntawm lub tsev menyuam, thymus, spleen thiab pituitary ntawm nas, ua raws li cov ntaub ntawv uas lub raum tsis txaus yog qhov tseem ceeb hauv MPS.
5. Cov lus xaus
Peb txoj kev tshawb fawb tau qhia tias PGC muaj cov txiaj ntsig zoo rau MPS-nws tuaj yeem ua rau muaj kev ywj pheej thiab muaj pes tsawg leeg ntawm kev sawv, lub sijhawm latent ntawm thawj zaug nkag mus rau hauv chav tsaus, viscera ntawm thymus, spleen, thiab uterus, E2, T. zoo li txo tus lej hluav taws xob, LH, thiab FSH. Cov phenylethanoid glycosides tau xaiv hauvCistancherau kev ua haujlwm siab thiab yooj yim ntawm MPS kev tshawb fawb los txhim kho kev kho mob perimenopausal syndrome. Nws cov qauv zoo yog yooj yim los tswj, ua rau kev tshawb fawb tshiab thiab kev tsim kho kev lag luam ntawm cov tshuaj. Nws muab txoj kev xav tshiab rau kev kho mob MPS.
