Neem nplooj (Azadirachta Indica A. Juss) Ethanolic Extract Ntawm Lub Siab thiab raum Kev Ua Haujlwm Ntawm nas
Mar 05, 2022
Taw qhia
txoj kev loj hlob ntawm cov tshuaj los ntawm tej yam ntuj tso khoom muaj nyob rau hauv lub ntiaj teb no cov xim niaj hnub no. cov khoom ntuj tsim muaj cov qauv tshuaj tshwj xeeb, tsim muaj ntau hom kev ua haujlwm lom neeg, thiab muaj cov tshuaj zoo li tshuaj [1]. ntxiv rau e kev coj noj coj ua ntawm cov tshuaj siv cov khoom ntuj tsim tau tshwm sim txij li lub sijhawm prehistoric hauv kev tiv thaiv thiab kho ntau yam kab mob. Cov khoom ntuj tsim muaj ntau yam zoo nyob rau hauv cov nqe lus ntawm kev ua tau zoo thiab kev xaiv ntawm lub hom phiaj molecule kom lawv tuaj yeem siv tau ntau zaus los muab cov tshuaj xav tau sai sai [2]. Cov khoom ntuj tsim muaj cov tshuaj nquag nrog kev cog qoob loo [8, 9], antihyperglycemic, antiulcer, antifungal, antibacterial, anti-inflammatory, immunomodulatory, antimutagenic, anticancer, antimalarial, antiviral, antioxidant [10-12], antifertility [13, 14], thiab kev tiv thaiv kab mob [9, 11]. Ntau qhov chaw ntawm cov nroj tsuag neem tau raug cais tawm zoo, muaj ntau dua 140 cov tshuaj sib xyaw ua ke [10], thiab tau siv los ua tshuaj ntsuab rau ntau txhiab xyoo [14]. Neem muaj ntau cov ntsiab lus tseem ceeb suav nrog cov roj derivatives, carbohydrates, thiab cov proteins thiab lwm yam sib txuas xws li flavonoids, steroids, saponins, terpenoids, alkaloids, glycosides, thiab tannins [7-15]. Neem nroj tsuag yog ib qho kev txaus siab thoob ntiaj teb vim tias lawv cov txiaj ntsig tsis muaj kev phiv. ntxiv rau e kev siv cov nroj tsuag neem ib txwm muaj kev nyab xeeb heev, thiab ntau dua 75 feem pua ntawm cov tshuaj ib txwm siv tshuaj ntsuab tshuaj ntsuab. ntxiv rau e keeb kwm ntawm nws txoj kev siv, los ntawm cov ntaub ntawv uas twb muaj lawm, tsis qhia txog kev phiv los ntawm nplooj neem. Txawm li cas los xij, kev ntsuam xyuas kev nyab xeeb ntawm kev siv cov nplooj ntoo nplooj tsis tau ua tiav [11].
CISTANCHE yuav txhim kho lub raum / raum ua haujlwm
Cov kev tshawb fawb yav dhau los tau tshaj tawm tias cov nplooj nplooj extract muaj tshuaj lom rau lub siab thiabraum [4, 11]; Txawm li cas los xij, lwm cov ntaub ntawv qhia tau hais tias cov nplooj nplooj nplooj tuaj yeem tiv thaiv lub siab thiabraumlos ntawm kev puas tsuaj [16–18]. Hauv Aceh Besar Regency, Aceh Province, Indonesia, cov neeg hauv zos tau siv nplooj neem ua zaub thiab siv los tiv thaiv cev xeeb tub yam tsis paub txog cov kev mob tshwm sim. ntxiv rau e kev nyab xeeb ntawm neem nplooj rau hnub no tseem tsis paub meej. Txog tam sim no, rau peb txoj kev paub, tseem muaj cov kev tshawb fawb tsawg uas ntsuas cov txiaj ntsig ntawm neem nplooj ethanolic extract ntawm daim siab thiablub raum ua haujlwm. Hauv txoj kev tshawb fawb tam sim no, lub hom phiaj yog los tshawb xyuas cov txiaj ntsig ntawm kev tswj hwm ntawm neem nplooj ethanolic extract ntawm daim siab thiablub raum ua haujlwmLos ntawm kev tshuaj xyuas cov concentration ntawm AST, ALT, urea, thiab creatinine thiab histopathological daim siab thiabraumhauv cov txiv neej nas.
Ntsiab lus:Lub raum ua haujlwm; Lub raum cov ntaub so ntswg; raum tsis ua haujlwm; Kab mob raum; Lub raum
Cov ntaub ntawv thiab cov txheej txheem
Neeb Nplooj Sample Collection.Cov nplooj neem tau sau los ntawm Kajhu Zos, Aceh Besar Regency, Aceh Province, Indonesia, thaum lub Cuaj Hli 2019. Kev txheeb xyuas cov nplooj neem tau ua tiav ntawm Bogoriense Herbarium, Biology Research Center, Indonesian Institute of Sciences (LIPI), Bogor, Indonesia (1454 /IPH.1.01/If.07/VII/2019), thiab nws yog qhia raws li Azadirachta indica A. Juss.Kev npaj ntawm Ethanol Extract ntawm Neem nplooj.Cov nplooj tshiab tau sau, ntxuav, cua qhuav, thiab mashed los tsim cov hmoov nplej. Tsis tas li ntawd, cov hmoov txhuv nplej siab tau macerated nrog ethanol 70 feem pua rau 7 hnub. Ntxiv rau, cov filtrate tau evaporated los ntawm kev siv cov evaporator teb ntawm 50 degree los tsim cov viscous extracts.Kev sim tsiaj.Cov tsiaj sim yog 24 txiv neej Wistar nas nrog 150–250 g lub cev hnyav. Cov nas tsuag tau acclimatized rau 7 hnub thiab muab faib ua plaub pawg (T0, T1, T2, thiab T3) raws li txhua pab pawg muaj 6 nas. T0 yog pab pawg tswj tsis muaj kev kho mob thiab T1, T2, thiab T3 yog pab pawg kho mob
muab cov neem nplooj ethanolic extract nrog 100, 200, thiab 300 mg / kg lub cev hnyav, feem. cov extract tau muab los ntawm qhov ncauj siv lub plab hnyuv rau 48 hnub, thiab kuaj ntshav rau hnub 49 los txiav txim siab qhov ntau ntawm AST, ALT, urea, thiab creatinine, thiab tom qab ntawd daim siab thiabraumCov ntaub so ntswg tau sau los ntsuas histopathology. Thaum txoj kev tshawb no, nas tau muab cov qauv pub thiab dej ad libitum.
Biochemical Analysis.Cov ntshav kuaj (2-3 ml) tau muab los ntawm lub plawv siv lub syringe sterile syringe. Ntxiv rau, cov ntshav tau pauv mus rau Eppendorf raj thiab sab laug ntawm chav tsev kub (30 degree) rau 10 feeb. Ntshav tau centrifuged rau 10 feeb ntawm 3000 rpm. ntxiv rau e ntshav tau muab cais thiab khaws cia rau hauv lub tub yees (0 20 degree ). Tsis tas li ntawd, AST, ALT, urea, thiab ntshav creatinine tau raug tshuaj xyuas los ntawm kev tshuaj ntsuam chemistry (Rayto RT-1904C). Ntxiv rau cov txheej txheem kev txiav txim siab ntawm AST, ALT, urea thiab creatinine concentrations tau ua raws li cov chaw tsim khoom raws tu qauv.
Kev kuaj Histopathology. The cev iraumCov nas tsuag tau kho nyob rau hauv 10 feem pua ntxhiab tsw buffered formalin tov rau 24 h, lub cev qhuav dej nyob rau hauv ib tug cawv series, thiab tshem tawm nyob rau hauv xylol tov tom qab embedded rau ntawm paraffin thaiv. Cov ntaub so ntswg embedded nyob rau hauv paraffin thaiv raug txiav siv ib tug teb microtome rau hauv 4 µm tuab, mounted rau iav slides, stained nrog hematoxylin thiab eosin, thiab soj ntsuam nyob rau hauv lub teeb lub teeb tsom ntawm ib tug magnification ntawm 10 × 10.Kev txheeb cais.Cov ntaub ntawv raug tshuaj xyuas los ntawm kev txheeb xyuas qhov sib txawv (ib-txoj kev ANOVA) ua raws li Duncan's posthoc test siv SPSS 24. A p-value<0.05 was="" considered="" statistically="">
CISTANCHE yuav txhim kho lub raum / raum mob
Cov txiaj ntsig thiab kev sib tham
6e AST thiab ALT Concentration. Ntxiv rau AST concentration ntawm T0, T1, T2, thiab T3 yog 290.9 ± 202.5 U/L, 277.2 ± 167.2 U/L, 421.3 ± 2{ {28}}1.5 U/L, thiab 366.3 ± 276.9 U/L, feem. ntxiv rau e concentration ntawm AST nyob rau hauv T2 thiab T3 pawg nyiam nce ntxiv piv rau T{{30}} (tswj pab pawg). Txawm li cas los xij, AST concentrations hauv T1, T2, thiab T3 pawg tsis pom qhov txawv ntawm T0 pawg (p> 0.05). Ntxiv rau e ALT concentration ntawm T0, T1, T2, thiab T3 yog 108.6 ± 23.1 U / L, 116.3 ± 51.9 U / L, 105.9 ± 15.2 U / L, thiab 95.9 ± 36.2 U / L, feem. ntxiv rau e concentration ntawm ALT nyob rau hauv T2 thiab T3 pawg tended txo qis piv rau T0 (tswj pab pawg).
Txawm li cas los xij, ALT concentrations hauv T1, T2, thiab T3 pawg tsis pom qhov txawv ntawm T0 pawg (p > 0.05) (Table 1). AST enzyme yog ib qho cim uas tau siv los ntsuas qhov muaj ntawm daim siab puas. Ntxiv rau yog enzyme ua lub luag haujlwm hauv cov txheej txheem ntawm gluconeogenesis los ua kom muaj kev hloov pauv ntawm aspartic acid amino pawg rau hauv ketoglutaric acid los tsim oxaloacetic acid. AST enzyme muaj nyob rau hauv cytosolic thiab mitochondrial isoenzymes ntawm daim siab, cov leeg pob txha, cov leeg plawv,lub raum,lub paj hlwb, pancreas, ntsws, leukocytes, thiab cov qe ntshav liab. Txawm li cas los xij, AST enzyme no tsis tshua nkag siab thiab tshwj xeeb rau kev ntsuas lub siab puas tsuaj [19]. Txawm hais tias tsis muaj qhov sib txawv tseem ceeb hauv AST cov ntsiab lus hauv cov pab pawg kho mob (T1, T2, thiab T3) piv rau pawg tswj hwm (T0), AST concentration nyiam nce hauv pawg T2 thiab T3 piv rau T. 0 (tswj pab pawg). ntxiv rau e nce tuaj yeem lig vim yog cov sib xyaw ua ke ntawm neem nplooj extracts xws li flavonoids, tannins, saponins, alkaloids, thiab steroids, tab sis cov active compounds ntawm neem tawm ethanol extract tsis tau txheeb xyuas kom nce AST. Kev nce hauv AST enzyme tsis tas yuav ua raws li lub siab puas tsuaj vim tias cov enzyme no tsis yog tshwj xeeb hauv daim siab nkaus xwb tab sis kuj nyob rau lwm cov ntaub so ntswg hauv lub cev.
Txoj kev tshawb no tseem pom tias ALT enzyme nyiam txo qis hauv pab pawg kho mob (T1, T2, thiab T3) piv rau pawg tswj hwm (T0), tab sis qhov txo qis ntawm ALT enzyme tsis yog qhov tseem ceeb. ALT enzymes yog ib qho kev kwv yees zoo dua ntawm daim siab kev puas tsuaj ntau dua li AST enzymes vim tias qhov cytosolic enzymes no tau pom nyob rau hauv qhov siab tshaj plaws nyob rau hauv daim siab thiab yog qhov tshwj xeeb tshaj plaws hauv kev ntsuam xyuas kev puas tsuaj ntawm daim siab ua haujlwm [20]. Ntxiv rau yog enzyme ua lub luag haujlwm hauv gluconeogenesis los ntawm kev ua kom hloov pauv ntawm cov amino pawg los ntawm alanine mus rau ketoglutaric acid los tsim pyruvic acid [19]. ALT muaj 496 amino acids thiab pom nyob rau hauv cytosol hepatocytes. ALT enzyme kev ua hauv lub siab yog kwv yees li 3000 lub sijhawm ntawm kev ua haujlwm hauv cov ntshav, yog li nyob rau hauv cov ntaub ntawv ntawm hepatocellular puas los yog tuag, ALT tso tawm los ntawm lub siab puas hlwb yuav ua rau cov kev ntsuas ntawm ALT enzyme nyob rau hauv cov ntshav [21].
CISTANCHE yuav txhim kho lub raum / raum mob ntshav qab zib
Mohamed et al. [22] pom tias ethanol extract ntawm nplooj neem ua rau txo qis hauv nas AST thiab ALT enzymes. Cov ethanol extract ntawm neem nplooj tsis muaj kev phiv rau daim siab, thiab nws txawm ua hauj lwm raws li lub siab tiv thaiv. Txoj kev tshawb no ua los ntawm Haque et al. [23] kuj pom tau hais tias cov txiv ntoo nplooj extract tsis ua rau muaj kev hloov pauv hauv cov ntshav ntshav ib txwm muaj ntawm AST thiab ALT enzymes hauv txiv neej nas. Bhangra et al. [24] tau tshaj tawm tias kev tswj hwm 500 mg / kg lub cev qhov hnyav ntawm cov nplooj nplooj extract hauv cov nas uas ua rau paracetamol txo qis kev puas siab ntawm daim siab, raws li qhia los ntawm qhov qub AST thiab ALT concentrations thiab kev soj ntsuam histopathological. Cov txiaj ntsig ntawm kev tshawb fawb ua los ntawm Dkhil et al. [17] qhia txog kev tiv thaiv ntawm methanolic extract ntawm neem nplooj nrog 500 mg / kg lub cev hnyav koob tshuaj rau lub siab ntawm cov nas induced los ntawm cisplatin. Mallick et al. [25] tau tshaj tawm tias cov tshuaj methanolic extract ntawm cov nplooj neem tsis muaj tshuaj lom rau lub siab nas txawm tias muaj ntau npaum li cas. Cov ntsiab lus antioxidant ntawm neem nplooj extract muab cov nyhuv hepatoprotective. Txoj kev tshawb no ua los ntawm Hartono thiab Prabowo [26] pom tias kev tswj hwm ethanol extract ntawm neem nplooj ntawm nas los ntawm inducing siab koob tshuaj paracetamol rau kaum hnub kuj yuav txo tau cov kev ua ntawm cov enzyme ALT nyob rau hauv kev sim tsiaj. Qhov txo qis hauv ALT enzyme yog vim muaj kev sib xyaw ua ke ntawm neem nplooj extract, uas ua haujlwm ua hepatoprotection los ntawm inhibiting oxidative daim siab puas. Cov tshuaj antioxidants tuaj yeem txo cov dawb radicals rau kev sib deev los ntawm kev khawb ncaj qha uas yog txo qhov tsim ntawm cov pa oxygen reactive (ROS) uas muaj tshuaj lom rau ntawm daim nyias nyias phospholipids thiab ua rau muaj kev puas tsuaj ntawm tes. Cov tshuaj antioxidants tuaj yeem txo oxidative kev nyuaj siab rau lub siab hlwb, thiab cov haujlwm ntawm ALT enzyme hauv cov ntshav yuav txo qis.
Urea thiab Creatinine Concentration.urea concentration ntawm T{{0}}, T1, T2, thiab T3 yog 51.1 ± 7.0 mg/dl, 55.6 ± 8.5 mg/dl, 44.5 ± 5.4 mg/dl, thiab 48.4 ± 3.4 mg / dl, feem. Urea concentrations hauv T1, T2, thiab T3 pawg tau pom qhov sib txawv tseem ceeb ntawm pawg T0 (p < 0.{{30}}5).="" creatinine="" concentration="" ntawm="" t0,="" t1,="" t2,="" thiab="" t3="" yog="" 0.22="" ±="" 0.{{40}}4="" mg/dl,="" {{44="" }}.24="" ±="" 003="" mg/dl,="" 0.21="" ±="" 0.03="" mg/dl,="" thiab="" 0.25="" ±="" 0.03="" mg/dl,="" raws="" creatinine="" concentrations="" hauv="" t1,="" t2,="" thiab="" t3="" pawg="" tsis="" pom="" qhov="" txawv="" ntawm="" t0="" pawg="" (p=""> 0.05) (Daim duab 1). Covraummuaj lub luag haujlwm tseem ceeb hauv kev tshem tawm cov khoom pov tseg thiab cov co toxins xws li urea, creatinine, thiab uric acid; Nws kuj muaj kev ua haujlwm hauv kev tswj hwm ntawm cov kua dej ntxiv, osmolality, electrolyte concentrations, thiab kev tsim cov tshuaj hormones. ntxiv rau e functional unit ntawm lubraumyog lub nephron uas muaj cov glomerulus, proximal tubules, distal tubules, thiab sau ducts [27]. ntxiv rau e cov txiaj ntsig tau pom tias muaj qhov sib txawv tseem ceeb hauv urea concentrations hauv pawg kho mob (T1, T2, thiab T3) piv rau pawg tswj hwm (T0), thiab urea concentration txo qis hauv pawg T2 thiab T3 piv. rau T0 (tswj pab pawg). Urea yog ib qho kev sib xyaw ua ke uas muaj nitrogen tsim hauv daim siab ua cov khoom kawg ntawm cov protein metabolism thiab lub voj voog urea. Kwv yees li ntawm 85 feem pua ntawm cov cheeb tsam raug tso tawm los ntawm covlub raum, thiab tus so yog excreted los ntawm digestive ib ntsuj av. Serum urea nce nyob rau hauv lub raum cleansing mob raws li nyob rau hauv mob thiab ntevraum tsis ua haujlwm. Urea kuj tuaj yeem nce ntxiv hauv lwm yam mob xws li mob plab hnyuv siab, lub cev qhuav dej, txheej txheem catabolic, thiab noj zaub mov muaj protein ntau [27].Hauv qhov kev tshawb fawb no, muaj qhov txo qis hauv cov concentration ntawm urea hauv pawg kho mob piv rau pawg tswj hwm. yog qhia tias ethanol extract ntawm neem nplooj tsis cuam tshuam raulub raum ua haujlwmntawm tus txiv neej nas. Raws li Guyton thiab Hall [28], tag nrho urea hauv cov ntshav tau txiav txim siab los ntawm kev noj zaub mov muaj protein ntau thiab muaj peev xwm ntawm cov urea hauv cov ntshav.lub raumtshem tawm urea. Yog tias ob lub raum puas lawm, urea yuav khaws cia hauv cov ntshav. Kev nce urea hauv plasma qhiaraum tsis ua haujlwmhauv kev ua nws txoj haujlwm filtration. plus e condition ofraum tsis ua haujlwm, uas yog tus cwj pwm los ntawm qib siab plasma urea, hu ua uremia.
Urea yog cov khoom kawg ntawm nitrogen tau los ntawm cov protein thiab amino acid catabolism, tsim los ntawm lub siab, thiab faib rau tag nrho cov kua dej hauv lub cev thiab cov cellular. Hauvraum, urea yog lim tawm ntawm cov ntshav los ntawm glomeruli thiab ib nrab reabsorbed los ntawm dej. Nce urea yog qhov qhia tau tias glomerular pom tus nqi tsis zoo. Kev nce urea concentration yog txuam nrogmob raumlos yog tsis ua hauj lwm, urinary tract obstruction los ntawmraumpob zeb, congestive plawv tsis ua hauj lwm, lub cev qhuav dej, kub taub hau, poob siab, thiab los ntshav nyob rau hauv lub digestive ib ntsuj av.Txoj kev tshawb no kuj pom tias tsis muaj qhov sib txawv tseem ceeb hauv creatinine concentrations hauv pawg kho mob (T1, T2, thiab T3) piv rau pawg tswj hwm (T0). tau qhia tias ethanol extract ntawm neem nplooj tsis ua rau cov nas puasraum.Creatinine feem ntau yog siv los ntsuaslub raum ua haujlwm. Creatinine concentration tsis yog tsuas yog cuam tshuam los ntawm cov khoom ntawm cov leeg nqaij tab sis kuj cuam tshuam los ntawm cov leeg nqaij, cov leeg nqaij, kev ua si, kev noj haus, thiab kev noj qab haus huv. Ntxiv creatinine concentrations kuj pom nyob rau hauv cov leeg nqaij dystrophy, anemia, leukemia, thiab hyperthyroidism [29]. Creatinine yog cov khoom siv feem ntau siv rau kev ntsuaslub raum ua haujlwm.creatinine concentration muab qhov qhia txog kev ua ntawm glomerulus. Creatinine yog ib qho byproduct ntawm creatine phosphate nyob rau hauv cov nqaij ntshiv thiab yog tsim nyob rau hauv ib tug ceev ceev los ntawm lub cev. Rau feem ntau, creatinine tshem tawm los ntawm cov ntshav tag nrholub raum. Kev nce creatinine qhia tau tias txo qis kev ntxuav los ntawm covlub raum. Creatinine serum yog qhov kev ntsuam xyuas tseeb dua ntawmlub raum ua haujlwmlos ntawm urea [27]
Monim et al. [16] pom tias kev tswj hwm cov nplooj nplooj extract ntawm koob tshuaj 500 mg / kg lub cev hnyav rau tsib hnub hauv cov nas tsis ua rau muaj kev hloov pauv hauv urea thiab creatinine concentrations hauv nas. Kepala et al. [30] kuj pom tau hais tias cov nas noj nrog cov nplooj nplooj extract ntawm 500 mg / kg lub cev hnyav npaum li cas rau 14 hnub ua rau txo qis hauv urea thiab creatinine concentrations hauv cov nas induced los ntawm cisplatin rau.raum puas. Cov ethanol extract ntawm neem nplooj ua raws li nephroprotection hauvraum puas.Kev tshawb fawb los ntawm Somsak et al. [18] pom tias neem nplooj dej extract ntawm koob tshuaj 1000 thiab 2000 mg / kg lub cev hnyav tsis muaj tshuaj lom rau nas. Neem nplooj extract tuaj yeem siv los ua tus neeg sib tw rau nephroprotection tiv thaiv kev puas tsuaj raulub raum.
Lub siab thiab lub raum Histopathology.Lub siab histopathological kev soj ntsuam hauv pawg tswj hwm (Daim duab 2(a) thiab pab pawg kho mob ntawm koob tshuaj 100 mg / kg (Daim duab 2(b)), 200 mg / kg (Daim duab 2(c)), thiab 300 mg / kg (Daim duab 2(d)) qhia tias lub siab, cov hlab ntsha hauv nruab nrab, thiab cov kab mob hepatocyte zoo li qub (Daim duab 2).raumKev soj ntsuam histopathology hauv pawg tswj hwm (Daim duab 3(a)) thiab pab pawg kho mob ntawm koob tshuaj 100 mg / kg (Daim duab 3(b)), 200 mg / kg (Daim duab 3(c)), thiab 300 mg / kg ( Daim duab 3(d)) tau pom ib txwm pom ntawm lub glomerulus thiab cov tubules ze ze thiab qhov chaw. ntxiv rau ere tsis muaj kev hloov pauv hauv histologicalraum(Daim duab 3).
Lub cev thiablub raumyog cov kabmob uas muaj lub luag haujlwm tseem ceeb hauv cov metabolism thiab tshem tawm cov tshuaj lossis lwm yam tshuaj. Hauv kev tshawb fawb, nws tau pom tias kev tswj hwm ntawm ethanol extract ntawm neem nplooj ntawm koob tshuaj 100, 200, thiab 300 mg / kg tsis ua rau muaj kev txawv txav thiab kev puas tsuaj rau daim siab nas. ntxiv rau e daim siab hauv cov nas kho tau pom cov qauv dav dav ntawm lub siab thiab ib txwm pom ntawm cov hlab ntsha hauv nruab nrab thiab cov hlwb hepatocyte. ntxiv rau e daim siab histopathology yog ua raws li cov txiaj ntsig ntawm kev tsom xam ntawm AST thiab ALT enzymes uas pom tias tsis muaj qhov sib txawv tseem ceeb ntawm pawg tswj hwm thiab kev kho mob. Txawm hais tias muaj qhov nce hauv AST enzyme hauv 200 thiab 300 mg / kg pawg piv rau pawg tswj hwm, lub siab histopathology zoo li qub. AST enzyme tsis yog tshwj xeeb rau lub siab puas tsuaj. AST feem ntau pom muaj nyob rau hauv cov qe ntshav liab, plawv thiab cov leeg pob txha, thiablub raum. AST tsis tshua nkag siab rau lub siab tshaj ALT [19].
Covraumhistopathology kev soj ntsuam tom qab kev tswj hwm ntawm ethanol extract ntawm neem tawm nrog koob tshuaj 100, 200, thiab 300 mg / kg tsis pom qhov txawv piv rau kev tswj hwm. Covraumnyob rau hauv cov nas kho tau pom qhov qubqauv ntawm lubraumthiab zoo li qub ntawm glomerulus thiab tubules. Qhov kev tshawb pom no tau txais kev txhawb nqa los ntawm kev txheeb xyuas ntawm urea thiab creatinine hauv cov ntshav nas uas tsis muaj qhov sib txawv tseem ceeb ntawm pawg tswj hwm thiab kev kho mob. Urea thiab creatinine yog cov tshuaj biochemical uas yog cov cim tseem ceeb ntawmlub raum ua haujlwm. Txawm li cas los xij, creatinine yog qhov kev ntsuam xyuas ntau dualub raum ua haujlwmlos ntawm urea [27] Qhov no yog nyob rau hauv txoj kab nrog qee cov ntaub ntawv dhau los uas cov nplooj nplooj extract muaj peev xwm hepatoprotective thiab nephroprotective cuam tshuam [31]. Kusuma et al. [32] pom tias neem nplooj ethanol extract tsis cuam tshuam rau hepatocyte txoj kab uas hla, siab lobules 'inch, thiab siab hnyav nyob rau hauv nas. Nyob rau hauv sib piv, txoj kev kawm ntawm Katsayal li al. [33] pom tias kev tswj hwm ntawm methanol extract ntawm neem nplooj ntawm koob tshuaj 500, 1000, thiab 2000 mg / kg ua rau muaj kev txawv txav hauv lub siab thiab ob lub raum ntawm cov nas kho mob piv rau kev tswj hwm. Qhov sib txawv ntawm qhov tshwm sim no tej zaum yog vim qhov sib txawv ntawm kev rho tawm ntawm cov nplooj neem. CovCov txiaj ntsig ntawm txoj kev tshawb no qhia tau hais tias ethanol extract ntawm neem nplooj los ntawm Kajhu tsis ua rau lub siab thiablub raumntawm txiv neej nas. Ntxiv rau yog vim lub active compound ntawm ethanol extract ntawm neem nplooj uas yog ib tug antioxidant. Cov kev tshawb fawb yav dhau los qhia tau hais tias neem nplooj ethanol extract los ntawm Kajhu muaj flavonoids, alkaloids, tannins, saponins, thiab steroids [34]. ntxiv rau e active compounds uas ua raws li antioxidants yog flavonoids. ntxiv rau e neem nplooj ethanol extract muaj zog antioxidant kev ua [35]. ntxiv rau peb, ethanol extract ntawm neem nplooj los ntawm Kajhu muaj kev nyab xeeb rau lub siab thiab ob lub raum ntawm txiv neej nas.
CISTANCHE yuav txhim kho lub raum / raum tsis ua haujlwm
Cov lus xaus
Cov ethanolic extract ntawm neem nplooj tsis ua kev puas tsuaj rau daim siab thiabraumNtawm cov txiv neej nas raws li kev txheeb xyuas ntawm AST, ALT enzymes, urea, thiab creatinine concentration thiab histopathological daim siab thiabraum. Txawm li cas los xij, kev tshawb nrhiav ntxiv yog xav tau rau kev ntsuam xyuas kev nyab xeeb ntawm cov nplooj nplooj nplooj ntawm ntau yam kom tau txais cov duab meej ntawm kev nyab xeeb ntawm cov nplooj neem rau cov tshuaj.
