Gao-Zi-Yao Txhim Kho Kev Kawm Thiab Nco Kev Ua Haujlwm hauv Qub Spontaneous Hypertensive Rats Part 3
Feb 19, 2024
Qhov cuam tshuam ntawm Gao-Zi-Yao ntawm Morris dej maze parametersin qub SHR
Morris dej maze cov ntaub ntawv qhia tau hais tias ob qho tib si nruab nrab thiab siab ntau npaum li cas ntawm Gao-Zi-Yao kev kho mob txo qis lub sijhawm latency ntawm thawj hnub kev cob qhia, txij hnub thib ob, qhov sib txawv tsis muaj nyob (Daim duab 8A).
Kev sib raug zoo ntawm kev khiav tawm latency thiab kev nco ib txwm yog lub ntsiab lus ntawm kev txhawj xeeb loj. Peb feem ntau xav tias peb lub cim xeeb zuj zus, tab sis nws nyuaj rau txheeb xyuas qhov tseeb. Kev khiav tawm ntawm cov neeg lurking feem ntau ua rau mob nco. Lawv zoo li tshem tawm lawv tus kheej los ntawm cov kev paub tsis zoo kom tawm ntawm qhov teeb meem, tab sis qhov no ua rau tib neeg lub hlwb lub zog thiab yog li ua rau nco tsis tau.
Kev khiav tawm los ntawm kev lurking yog ib txoj hauv kev rau tib neeg los tiv thaiv kev ntxhov siab, thiab cov txiaj ntsig luv luv uas nws coj tau pom tseeb. Los ntawm kev khiav tawm cov kev paub tsis zoo, ib tus neeg tuaj yeem hnov zoo ib ntus. Tab sis txoj hauv kev no tseem tsis muaj kev kawm tseem ceeb. Thaum peb ntsib kev nyuaj lossis kev poob qis, peb feem ntau sim tsis nco qab cov kev paub no, zoo li zais lawv tob rau hauv. Tab sis qhov tseeb, cov kev paub dhau los thiab cov lus qhia coj los ntawm cov kev paub no muaj txiaj ntsig ntau tshaj qhov peb xav tau. Tsuas yog ntsib koj cov teeb meem tiag tiag koj tuaj yeem tau txais lub zog thiab kev txawj ntse los ntawm lawv.
Yog li ntawd, ntsib teeb meem zoo yog tus yuam sij rau kev txhim kho kev nco. Qee qhov kev tshawb fawb tau pom tias tib neeg lub peev xwm nco tau cuam tshuam los ntawm kev paub. Thaum peb nyob rau hauv kev sib tw hom, lub hlwb yuav cia li nrhiav tau qhov kev daws teeb meem los txhim kho kev nco.
Hauv lub neej niaj hnub no, peb feem ntau ntsib qee qhov kev nyuaj thiab nyuaj, uas yuav tsum tau ntsib thiab peb tsis tuaj yeem dim xwb. Tsuas yog los ntawm kev koom tes nrog cov kev cov nyom no tuaj yeem qhib cov tswv yim, tshawb nrhiav cov kev daws teeb meem uas haum rau koj, thiab siv koj lub cim xeeb thiab kev muaj tswv yim zoo dua. Yog li ntawd, tsuas yog los ntawm kev ua siab ntev ntsib koj cov teeb meem thiab nquag nrhiav kev daws teeb meem tuaj yeem txhim kho koj lub cim xeeb thiab lub neej zoo. Nws tuaj yeem pom tau tias peb yuav tsum txhim kho kev nco, thiab Cistanche deserticola tuaj yeem txhim kho kev nco, vim Cistanche deserticola tseem tuaj yeem tswj hwm qhov sib npaug ntawm cov neurotransmitters, xws li nce qib ntawm acetylcholine thiab kev loj hlob. Cov khoom no tseem ceeb heev rau kev nco thiab kev kawm. Tsis tas li ntawd, Cistanche deserticola tseem tuaj yeem txhim kho cov ntshav khiav thiab txhawb nqa cov pa oxygen, uas tuaj yeem ua kom lub hlwb tau txais cov as-ham txaus thiab lub zog, yog li txhim kho lub hlwb tseem ceeb thiab kev ua siab ntev.
Nyem paub 10 txoj hauv kev los txhim kho kev nco
Lub sij hawm hla lub hom phiaj quadrant tau nce ntau ntxiv nyob rau hauv txhua pab pawg kho mob hnub 6 piv nrog rau pawg tswj hwm (Daim duab 8B).
Tsuas yog kev kho mob Gao-Zi-Yao siab ntau tau nce qhov feem pua ntawm lub sijhawm ntawm lub hom phiaj platform quadrant thiab feem pua ntawm txoj kev ntev hauv quadrant (Fig. 8C, D). Cov txiaj ntsig no qhia tias Gao-Zi-Yao tuaj yeem kho qhov poob ntawm kev kawm thiab kev nco ua haujlwm hauv SHR qub.
Cov nyhuv ntawm Gao-Zi-Yao ntawm tus naj npawb ntawm neuronsin hippocampus hauv SHR qub
Nissl staining tau ua kom pom cov naj npawb ntawm cov neurons hauv hippocampus (Fig. 9A). Cov txiaj ntsig tau pom tias kev kho mob nrog Gao-Zi-Yao nrog tag nrho cov koob tshuaj tsis muaj txiaj ntsig rau ntau lub paj hlwb hauv CA1 thiab CA2areas hauv hippocampus (Fig. 9B, C).
Txawm li cas los xij, tus naj npawb ntawm cov neurons hauv cheeb tsam DG tau nce ntxiv los ntawm qhov nruab nrab thiab siab ntau ntawm kev kho Gao-Zi-Yao, tab sis tsis yog qhov qis ntawm Gao-Zi-Yao (Daim duab 9D). Tese cov ntaub ntawv qhiaGao-Zi-Yao tuaj yeem tiv thaiv kev laus cuam tshuam txog neuron poob hauv SHR.
Cov txiaj ntsig ntawm Gao-Zi-Yao ntawm kev qhia ntawm kev kawm thiab kev nco txog cov protein ntawm hippocampus hauv SHR qub
Western blot cov txiaj ntsig tau pom tias kev qhia ntawm GluR1protein tau nce los ntawm kev siv ntau ntawm Gao-Zi-Yao (Fig. 10A), NMDAR 2B protein qhia tau nce los ntawm tag nrho cov koob tshuaj ntawm Gao-Zi-Yao (Fig. 10B), phosphorylatedCaMK II tau nce los ntawm tag nrho cov koob tshuaj ntawm Gao-Zi-Yao (Fig. 10C), thiab phosphorylated-CREB tau nce los ntawm ntau npaum li cas ntawm Gao-Zi-Yao (Fig. 10D).
Cov txiaj ntsig no qhia tias Gao-Zi-Yao tuaj yeem txhim kho synaptic plasticity los ntawm kev tswj hwm synaptic plasticity-txog proteinexpressions.
Kev sib tham
Hauv txoj kev tshawb fawb tam sim no, peb qhov kev soj ntsuam tau qhia tias Gao-ZiYao tawm dag zog los tiv thaiv hypertensive thiab txhim kho kev kawm thiab kev nco ua haujlwm ntawm SHR qub, uas tej zaum yuav yog los ntawm kev tswj hwm ntawm oxidative kev nyuaj siab, inflammatory yam, neuronnumber nyob rau hauv hippocampal DG cheeb tsam thiab qhia txog kev ua haujlwm. ntawm kev kawm thiab kev nco txog cov proteins.
Cov pov thawj ntxiv qhia tau hais tias muaj kev sib raug zoo ntawm kev laus thiab kub siab thiab nws tau raug tshuaj xyuas zoo [40].
Kev laus ua rau muaj kev ntxhov siab oxidative, uas yog ib qho tseem ceeb hauv kev txhim kho ntshav siab. Cov ntaub ntawv uas twb muaj lawm tau pom tias kev kho mob antioxidant los txo cov kev ntxhov siab oxidative tiv thaiv lub hnub nyoog txog kev loj hlob ntawm cov ntshav siab hauv SHR [41, 42].
Peb GaoZi-Yao tam sim no muaj ntau yam tshuaj ntsuab uas muaj cov teebmeem antioxidant thiab tuaj yeem tswj cov ntaub so ntswg oxidative kev nyuaj siab, xws li Fructus Corni, Semen Cuscutae, Rhizoma Gastrodiae, RadixCyathulae, Rhizoma Atractylodis Macrocephalae, Amomum villosum thiab Crocus sativus [16–21, 21].
Peb cov ntaub ntawv tam sim no tau qhia meej tias Gao-Zi-Yao tuaj yeem nce qib ntawm NO, thiab txo cov ntshav siab systolic hauv oldSHR, qhia tias cov khoom tiv thaiv ntshav siab ntawm GAOZi-Yao tuaj yeem yog vim nws cov nyhuv antioxidant.
Txawm hais tias kev sim tshuaj tsis ntev los no tau pom tias tsuas yog ob feem peb ntawm Alzheimer's dementia mob yog tshwm sim los ntawm cov hnub nyoog ntsig txog neuropathology [43], agingis feem ntau lees txais los ua ib qho tseem ceeb hauv kev loj hlob ntawm dementia [44, 45]. Lub hnub nyoog-dependentmemory impairments nyob rau hauv cov qauv tsiaj tau tshaj tawm [46].Ntau cov pov thawj pom tias muaj kev paub txog kev coj cwj pwm tsis zoo hauv cov qauv tsiaj dementia [47].
Lub caij no, kev kub siab tsis ntev los no tau txuas nrog Alzheimer's tus kab mob loj ua rau dementia hauv cov neeg laus, kev kub siab kuj tseem tuaj yeem txhawb nqa cov kab mob neurodegenerative hauv qab Alzheimer's disease [48].Cov kev sim tshuaj tsis ntev los no kuj tau qhia tias kev txhim kho hypertension txo qhov kev pheej hmoo ntawm kev paub tsis meej thiab kev dementia [49] .
Hauv txoj kev tshawb fawb tam sim no, weobserved kuj tseem muaj kev poob qis ntawm kev kawm thiab kev nco ua haujlwm hauv SHR qub, uas tau ua raws li kev soj ntsuam yav dhau los tau tshaj tawm tias kev ua haujlwm nco thiab kev kawm tau pom tias muaj kev cuam tshuam los ntawm kev laus [50]. Tsis tas li ntawd, nws tau tshaj tawm tias qhov ua tau zoo ntawm Morriswater maze kev ua tau zoo tau pom nyob rau hauv 23-lub limtiam SHR piv nrog cov hnub nyoog sib piv SD nas [51].
Ua ke, cov pov thawj uas twb muaj lawm qhia tau hais tias muaj kev poob qis hauv kev kawm thiab kev nco ua haujlwm hauv SHR qub. Kev laus ua ke nrog kev kub siab yog qhov tseem ceeb rau kev loj hlob ntawm dementia. Gao-Zi-Yao exerts nws muaj nuj nqi ib feem los ntawm nws cov antihypertensive zog.
Tsis tas li ntawd, kev puas tsuaj oxidative yog ib feem tseem ceeb ntawm Alzheimer's kab mob etiology thiab pathogenesis [52], Gao-Zi-Yaoincreases systemic NO, uas kuj yuav pab txhawb ameliorating lub cerebral system. Muaj kev loj hlob lub cev ntawm cov pov thawj tias ob qho tib si hauv zos thiab systemicinflammation tseem ceeb hauv kev dementia [53], peb cov ntaub ntawv tam sim no kuj pom tau tias Gao-Zi-Yao siv cov kev cai cuam tshuam rau cov kab mob hauv lub cev los ntawm kev txo qis ntawm IL-1 , IL{{7 }}, thiab.
TNF-, uas tseem tuaj yeem ua lub luag haujlwm hauv cov qauv tsiaj tam sim no. Tsis tas li ntawd, lub hnub nyoog hais txog kev poob qis ntawm cov neurons tau pom cov tsiaj [54], kev laus ua rau muaj qhov txo qis ntawm Nisslbody hauv hippocampal CA1 thiab CA3 cheeb tsam insenescence-accelerated nas [55], hauv txoj kev tshawb no, peb kuj tau pom. uas yog qhov poob ntawm cov naj npawb neuron hauv hippocampus hauv cov laus SHR piv nrog cov tub ntxhais hluas SHR, qhia txog kev ua tsis taus ntawm kev kawm thiab kev nco inold SHR tej zaum yuav yog vim qhov poob ntawm neuron. Muaj qhov cuam tshuam loj heev nyob rau lub sijhawm ntev nyob rau hauv nruab nrab-hnub nyoog nas hippocampus slices piv nrog cov nas hluas, thiab qhov txo qis ntawm GluR1, thiab NMDAR protein qhia, qhia txog kev puas tsuaj synaptic plasticity los ntawm kev laus [56].
Lwm cov cim ntawm synaptic plasticity, calmodulin-dependent protein kinase II (CaMKII) thiab phosphorylated CaMKII, CREB, thiab phosphorylated CREB, kuj tau tshaj tawm tias muaj feem cuam tshuam txog kev laus [57]. Peb txoj kev tshawb fawb tam sim no kuj qhia tau tias muaj qis dua ntawm cov kab mob synaptic plasticity-related protein qhia (GluR1, NMDAR 2B, phosphorylated CaMKII, phosphorylated CREB) hauv SHR qub, qhia tias tseem muaj qhov poob ntawm synaptic plasticity, uas yuav ua rau muaj kev cuam tshuam ntawm kev kawm thiab Lub cim xeeb ua haujlwm hauv SHR qub.Ntau cov pov thawj pom tau tias TCM therapy muaj peev xwm cuam tshuam rau kev txhim kho kev txawj ntse [58]. Randomized tswj cov ntaub ntawv tau txais kev txhawb nqa qhov txiaj ntsig zoo ntawm TCM ntawm kev nco txog hnub nyoog cuam tshuam [59].
Ntau qhov kev sim tshuaj ntsuam xyuas kuj tau pom qhov txiaj ntsig zoo ntawm TCM ntawm kev txhim kho kev nco hauv cov neeg mob laus lossis cov neeg noj qab haus huv [60,61]. Ntau cov tshuaj ntsuab suav nrog hauv daim ntawv tshuaj tam sim no tau ua kom pom los txhim kho kev txawj ntse. Loganin yog ib qho tseem ceeb iridoid glycoside tau los ntawm Corni fructus uas txhim kho lub zog mus sij hawm ntev thiab rov ua kom rov qab tau scopolamine-vim kev kawm thiab nco tsis tau [62].
Semen Cuscutae attenuates scopolamine-induced nco deficit nyob rau hauv nas [63]. Gastrodin, anactive tivthaiv cais los ntawm Rhizome Gastrodiae, txhim kho kev nco tsis zoo hauv Morris dej maze kuaj hauv nas [64].
Rhizoma AtractylodisMacrocephalae muaj cov tshuaj muaj kev tiv thaiv tiv thaiv ischemia-induced neuronal thiab kev paub tsis meej [65]. -Isocubebenol thiab deoxyschisandrin raug cais tawm los ntawm Fructus Schisandrae Chinensis thiab pom muaj kev tiv thaiv los ntawm kev puas hlwb [66,67]. Semen Ziziphi Spinosae ameliorates nco thiab kawm kev kawm hauv nas [23, 68]. MSS, uas suav nrog kev xa ntawv (Prunus mume Sieb. et Zucc) mloog zoo, disodium succinate thiab Span80 (3.6: 4.6: 1 piv) tau pom qhov kev txhim kho tseem ceeb hauv kev nco hauv nas [69].
Crocus sativus L. extracts antagonize nco impairments nyob rau hauv txawv kev coj cwj pwm ua hauj lwm nyob rau hauv tus nas [70]. Panaxnotoginseng attenuates kev puas tsuaj ntawm kev kawm thiab kev nco nyob rau hauv lub sij hawm ntev ischemia-reperfusion raug mob nas[71] thiab hauv A (1-42)-injected nas [72]. Radix Ophiopogonis, ib feem ntawm Shengmaisan, txhim kho kev kawm thiab nco lub peev xwm ntawm cov nas [73]. Berberine, ib qho naturalisoquinoline alkaloid cais los ntawm Rhizoma coptidis, txo qis kev txawj ntse hauv Alzheimer's Disease nas qauv [74, 75].
Tag nrho cov ntaub ntawv hais saum toj no txhawb nqa tias Gao-Zi-Yao kuj tseem tuaj yeem txhim kho kev kawm thiab kev nco hauv SHR qub. Peb cov ntaub ntawv qhia tau hais tias kev kho Gao-Zi-Yao txo qis systolic ntshav siab, tswj oxidative kev nyuaj siab thiab o, txhim kho kev kawm thiab nco, thiab up-regulates ntau neuronsand synaptic plasticity-txog cov protein qhia. Cov ntaub ntawv no tau lees paub tias Gao-Zi-Yao tuaj yeem ua rau muaj kev tiv thaiv neuroprotective.
Kev txwv
Nws yuav tsum raug sau tseg tias txawm tias qhov txo qis hauv cov ntshav siab pab txhawb kev txhim kho kev txawj ntse tsis suav nrog hauv qhov kev soj ntsuam tam sim no.Vim tias ntshav siab tau pom tias yog qhov tsis txaus ntseeg hauv kev txhim kho dementia, itis tsim nyog xav tias qhov txo qis hauv ntshav siab. kuj tseem tuaj yeem ua lub luag haujlwm hauv kev txhim kho kev txawj ntse.
Cov lus xaus
Hauv kev xaus, peb txoj kev tshawb fawb tam sim no qhia txog cov tshuaj tiv thaiv kab mob thiab cov kab mob neuroprotective ntawm Gao-Zi-Yao.Peb cov ntaub ntawv yuav muab pov thawj yooj yim rau kev siv tshuaj kho mob ntawm Gao-Zi-Yao hauv kev kho cov neeg mob ntshav siab.
Cov ntawv luv
SHR: Spontaneous hypertensive nas; AD: Alzheimer's disease; TCM: Tshuaj suav tshuaj; SBP: Systolic ntshav siab; GluR1: Glutamate receptorA1; NMDAR: N-methyl-d-aspartate receptor; CaMKII: Calmodulin-dependentprotein kinase II; p-CREB: Phosphorylated-cAMP responsive element-bindingprotein; DG: Dentate gyrus.
Kev lees paub
Tsis siv tau
Cov neeg sau ntawv pab txhawb
Meng-Xiao Han, Wen-Yi Jiang, Yan Jian, Lin-Hui Wang, thiab Rong Xue ib qho kev tshawb fawb. Guo-Xing Zhang txheeb xyuas cov ntaub ntawv thiab sau ntawv. JingWei Chen tsim kev tshawb fawb. Tus kws sau ntawv tau nyeem thiab pom zoo cov ntawv kawg.
Nyiaj txiag
Txoj haujlwm no tau txais kev txhawb nqa los ntawm National Natural Science Foundation ntawm Tuam Tshoj (81970422), Suzhou Municipal Science thiab Technology Bureau (SS201745, GSWS2019022), thiab 5th 333 Project ntawm Jiangsu Province.
Muaj cov ntaub ntawv thiab cov ntaub ntawv
Tag nrho cov ntaub ntawv tsim los yog tshuaj xyuas thaum lub sijhawm kawm no suav nrog hauv tsab xov xwm no.
Cov lus tshaj tawm
Kev pom zoo ntawm kev ncaj ncees thiab kev tso cai los koom
Cov kev sim no tau ua raws li National Institutes of Health Guidelines for the Use of Laboratory Tsiaj (NIH, luam tawm naj npawb 85–23, hloov kho 1996.), uas tau pom zoo los ntawm thiab ua raws li cov lus qhia rau kev saib xyuas thiab siv tsiaj txhu tsim los ntawm ethics licensingcommittee ntawm Soochow University. Tag nrho cov tshuaj ntsuab thiab lwm yam khoom siv hauv kev npaj ntawm Gao-Zi Yao tau pom zoo los ntawm National Health Commission of the People's Republic of China. Txoj kev tshawb no tau tshaj tawm los ntawm ARRIVE cov lus qhia.
Tso cai rau kev tshaj tawm
Tsis siv tau.
Kev nyiam sib tw
Tsis siv tau.
Sau Paub meej
1 Department of Physiology, Medical College ntawm Soochow University, 199Ren-Ai Road, Dushu Lake Campus, Suzhou Industrial Park, 215123 Suzhou, Tib neeg lub koom pheej ntawm Tuam Tshoj. 2Suzhou Key Laboratory of Drug Research for Prevention and Treatment of Hyperlipidemic Diseases, Medical Collegeof Soochow University, 199 Ren-Ai Road, Dushu Lake Campus, Suzhou Industrial Park, Suzhou 215123, Tib neeg koom pheej ntawm Tuam Tshoj. 3 Department of InternalMedicine, Affiliated Suzhou Chinese Traditional Medicine Hospital, NanjingUniversity of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, People'sRepublic of China.
Cov ntaub ntawv
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