Gao-Zi-Yao Txhim Kho Kev Kawm Thiab Nco Kev Ua Haujlwm hauv Qub Spontaneous Hypertensive Rats Part 1

Abstract

Lub hom phiaj: Gao-Zi-Yao tau ntev ua ib txoj hauv kev kho ntau yam kab mob. Txoj kev tshawb fawb tam sim no yog tshawb nrhiav cov txiaj ntsig ntawm Gao-Zi-Yao ntawm kev kawm thiab kev nco ua haujlwm hauv cov laus spontaneous hypertensive nas (SHR) thiab nws cov txheej txheem ua tau.

Thaum lawv muaj hnub nyoog, ntau tus neeg pom tias lawv lub cim xeeb pib maj mam poob. Txawm li cas los xij, ib txoj kev tshawb fawb tshiab qhia tau hais tias kev kub siab tshwm sim tsis ua rau kev nco. Hloov chaw, txoj kev tshawb no qhia tias ntshav siab tuaj yeem txuas rau kev nco zoo dua.

Cov kws tshawb fawb tau tshuaj xyuas ntau dua 1,000 cov neeg laus dua 40 xyoo, ib nrab ntawm cov neeg muaj ntshav siab. Cov kev tshawb pom tau pom tias cov neeg muaj ntshav siab ua tau zoo dua ntawm ntau yam kev ntsuam xyuas nco.

Txawm hais tias tsis muaj kev txiav txim siab tsis tau raug txiav txim siab, cov txiaj ntsig tau qhia tias ntshav siab yuav tsis yog qhov ua rau tsis nco qab. Hloov chaw, nws yuav qhia tau tias nrog kev tswj ntshav siab zoo, tib neeg tuaj yeem txhim kho lawv txoj kev nco.

Nws yog ib qho tsim nyog yuav tsum nco ntsoov tias ntshav siab yuav ua rau lwm yam kev puas tsuaj rau lub hlwb thiab lub cev (xws li mob stroke, kab mob plawv, thiab lwm yam), yog li nws tseem yuav tsum tau ntsuas ntxov kom tswj cov ntshav siab.

Txoj kev tshawb no coj qee cov xov xwm zoo rau cov neeg uas muaj ntshav siab: lawv tuaj yeem ua kom nco tau zoo hauv ntau yam. Yog li muaj xov xwm zoo rau cov neeg txhawj xeeb txog ntshav siab cuam tshuam rau lawv lub cim xeeb, thiab lawv tuaj yeem ua cov kauj ruam los tswj lub neej noj qab haus huv thiab qib ntshav siab. Nws tuaj yeem pom tau tias peb yuav tsum txhim kho kev nco, thiab Cistanche deserticola tuaj yeem txhim kho kev nco zoo vim Cistanche deserticola yog cov khoom siv tshuaj suav tshuaj uas muaj ntau yam teebmeem, ib qho ntawm kev txhim kho kev nco. Kev ua tau zoo ntawm Cistanche deserticola los ntawm ntau yam khoom xyaw uas nws muaj, suav nrog tannic acid, polysaccharides, flavonoid glycosides, thiab lwm yam. Cov khoom xyaw no tuaj yeem txhawb lub hlwb kev noj qab haus huv los ntawm ntau txoj hauv kev.

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Nyem Paub Txoj Kev Txhim Kho Kev Nco

Txoj Kev: Txiv neej SHR tau txais ntau koob tshuaj Gao-Zi-Yao rau 4 lub lis piam. Systolic ntshav siab (SBP) thiab lub plawv dhia tau saib xyuas. Cov ntshav qib ntawm nitric oxide (NO), interleukin (IL)-1 , IL-2, thiab qog necrotic factor (TNF)- tau ntsuas.

Morris dej maze tau ua los ntsuas kev kawm thiab kev nco ua haujlwm ntawm cov nas. Ntau cov neurons hauv hippocampus tau suav los ntawm Nissl staining.

Western blot tau siv los txheeb xyuas cov kev qhia ntawm kev kawm thiab kev nco txog kev ua haujlwm ntawm cov proteins, N-methyl-d-aspartate receptor 2B (NMDAR 2B), glutamate receptor 1 (GluR1), phosphorylated-calmodulin-dependent protein kinase II (p-CaMK II. ), thiab phosphorylated-cAMP responsiveelement-binding protein (p-CREB) hauv nas hippocampus.

Cov txiaj ntsig:

Cov ntaub ntawv qhia tias Gao-Zi-Yao txo SBP hauv SHR qub, nce NO theem, thiab txo qis qib ntawm IL-1 , IL-2, thiab TNF- . Qhov kev sim dej Morris tshawb pom tau pom tias Gao-Zi-Yao koob tshuaj tau txhim kho kev kawm thiab kev nco.

Tus naj npawb ntawm cov neurons hauv thaj tsam hippocampal dentate gyrus (DG) ntawm lub qub SHR tau nce los ntawm kev kho Gao-Zi-Yao. Tsis tas li ntawd, Gao-Zi-Yao txhawb nqa cov lus qhia protein ntawm NMDAR 2B, GluR1, p-CaMK II, thiab p-CREB hauv hippocampus.

Xaus: Gao-Zi-Yao txo SBP thiab txhim kho kev kawm thiab kev nco ua haujlwm ntawm SHR qub los ntawm kev tswj hwm oxidative kev nyuaj siab, cov kab mob inflammatory, thiab cov xov tooj ntawm cov neuron hauv cheeb tsam hippocampal DG thiab kev qhia txog kev kawm thiab kev nco txog cov proteins.

Cov lus tseem ceeb: Gao-Zi-Yao (qhov ncauj tshuaj ntsuab muab tshuaj txhuam), Spontaneous hypertensive nas (SHR), Kawm thiab nco

Taw qhia

Kev laus tau pom tias muaj feem cuam tshuam nrog kev poob qis hauv kev txawj ntse hauv cov ntsiab lus ntawm kev nkag siab, kev xav, thiab kev ua haujlwm nco [1–3].

Kev laus tau pom zoo los ua lub luag haujlwm tseem ceeb hauv cov kab mob pathogenesis thiab kev loj hlob ntawm ib qho ntawm cov kab mob uas muaj hnub nyoog tshaj plaws, dementia (Alzheimer's disease, AD) ntawm oxidativestress teeb liab txoj kev [4, 5].

AD yog hom dementia ntau tshaj plaws thiab feem ntau tshwm sim los ntawm kev poob qis ntawm episodic nco thiab kev paub txog kev ua haujlwm, tom qab ntawd ua rau cov lus thiab cov kev txawj visuospatial tsis txaus, uas feem ntau nrog cov kev coj cwj pwm tsis zoo xws li apathy, aggressiveness, thiab kev nyuaj siab [6, 7]. Tsis tas li ntawd, nws tau raug lees paub tias kev kub siab yog ib qho ntawm cov kev pheej hmoo loj rau dementia [8].

Hypertensive kev sib tw nce qhov kev qhia ntawm tus neeg txais txiaj ntsig rau cov khoom siv qib siab glycated kawg, ua rau tobeta-amyloid (A) deposition (ib qho ntawm cov txheej txheem pathogenesis ntawm AD) thiab kev kawm tsis zoo [9, 10]. Vim yog qhov nyuaj ntawm kev laus-induced dementia ua ke nrog ntshav siab, tseem tsis muaj lub tswv yim txaus siab rau cov neeg mob AD mob ntshav siab hauv tsev kho mob.

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Cov tshuaj suav tshuaj (TCM) muaj ntau tshaj 2, 000 xyoo keeb kwm thiab tau txais kev siv tshuaj kho mob thoob plaws. Cov teebmeem ntawm TCM ntawm kev tiv thaiv kev laus tau lees paub thoob plaws thiab tau raug tshuaj xyuas zoo [11].

Kev sim tshuaj hauv Suav teb tau pom tias TCMenhances cov neeg laus hippocampal neurogenesis los ntawm kev ua kom muaj ntau txoj hauv kev [12]. Kev tshawb fawb Randomizedclinical hauv Tebchaws Europe kuj tau qhia txog kev txhim kho ntawm kev paub tsis meej ntawm cov neeg mob AD los ntawm kev kho mob TCM [13].

Tsis tas li ntawd, TCM tau pom zoo kom txo cov ntshav siab hauv cov neeg mob [14]. Randomized tswj kev sim kuj pom tau tias muaj qee yam tshuaj tiv thaiv kab mob siab thiab muaj kev nyab xeeb zoo ntawm TCM, thiab tej zaum yuav yog lwm txoj hauv kev rau cov neeg mob ntshav siab [15] .Gaofang, ib qho ntawm cov tshuaj ntsuab hauv qhov ncauj pastes, yog ib hom tshuaj suav tshuaj suav tshuaj. Gao txhais tau hais tias herbalpaste thiab fang luv luv rau tshuaj.

Gao-Zi-Yaorefers rau lub qhov ncauj tshuaj ntsuab muab tshuaj txhuam raws li cov tshuaj tshwj xeeb, yog tsim los ntawm ntau yam tshuaj ntsuab, thiab muaj txiaj ntsig zoo rau kev tiv thaiv kab mob, tiv thaiv kab mob, thiab kev noj qab haus huv. Gao-Zi-Yao tau siv rau kev kho mob ntawm ntau yam kab mob xws li kev noj qab haus huv, tsis muaj zog, anemia, hnoos ntev, kev coj khaub ncaws, thiab lwm yam rau ob txhiab xyoo.

Cov qauv tam sim no ntawm Gao-Zi-Yao (Table 1) yog hloov kho los ntawm tus kws kho mob ntawm Wu Therapy ntawm TCM, Tian-shi Ye (1666-1745, Qing Dynasty).

Raws li cov ntaub ntawv uas twb muaj lawm, tshuaj ntsuab uas muaj nyob rau hauv cov mis no muaj cov teebmeem nram qab no: antioxidant thiab neuroprotective kev ua ub no ntawm Fructus Corni tau pom [16], Semen Cuscutaehas tau pom los tswj lub cev tsis muaj zog [17], Infammatory teb yuav raug tswj los ntawm Rhizoma Gastrodiae, Radix Cyathulae, Rhizoma Atractylodis Macrocephalae, thiab Fructus Amomi Villosi [18–21].

Fructus Schisandrae Chinensis qhia txog kev tiv thaiv kev laus [22], Semen Ziziphi Spinosae tau ua pov thawj rau ameliorateneuronal mob [23], mob nkees nkees tuaj yeem txhim kho los ntawm Radix Pseudostellariae [24], plusmum exerts inhibitory cuam tshuam ntawm cov khoom siv hauv aldivusre [25] txo oxidative kev nyuaj siab [26], Panax Notoginseng muaj tseeb cuam tshuam rau kev tiv thaiv kev laus thiab kev laus cov kab mob [27], Rhizome PinelliaePreparata txhawb kev pw tsaug zog los ntawm kev nce tus naj npawb ntawm qhov muag nrawm (REM) pw tsaug zog [28].

Alismatis Rhizoma muaj diuretic, antimetabolic teeb meem, hepatoprotective, immunomodulatory, antiosteoporotic, anti-inflammatory, antitumor, antibacterial, thiab antiviral kev ua ub no [29], Radix Ophiopogonis muaj ib qho kev tiv thaiv mob ntev ntawm cov hlwb senescent [30], Coptidisoma nrog Rhizoma. , ScutellariaeRadix, qhia cov nyhuv antihypertensive [31], Colla CoriiAsini txhim kho antioxidant thiab tiv thaiv kev ua ub no [32].

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Colla Carapacis thiab Plastri Testudinis muaj ntau yam amino acids thiab hlau ntsiab [33]. Yuan et al.observed catalog, ib qho khoom xyaw nquag ntawm Rehmanniaeradix preparata, uas yog cov tshuaj suav siv ntau tshaj plaws hauv Suav teb tau zoo ameliorates hyperactive thiab impulsive cwj pwm, thiab txhim kho spatial kev kawm thiab nco hauv SHR [34].

Tsis ntev los no, los ntawm kev siv cov tshuaj, peb tau pom tias Gao-Zi-Yao siv tshuaj tiv thaiv ntshav siab thiab tiv thaiv kab mob plawv-kev kho mob rau cov neeg lausSHR [35]. Kev kub siab tuaj yeem ua rau cov hlab ntsha me me thiab ib nrab ntawm cov teeb meem dawb degeneration nyob rau hauv lub hlwb, SHRs qhia kev paub tsis meej nrog lub hnub nyoog nce [36].Txawm li cas los xij, cov txiaj ntsig ntawm Gao-Zi-Yao tam sim no ntawm kev kawm thiab kev nco ua haujlwm hauv cov qauv kub siab qub tseem tsis tau paub.

Hauv qhov kev soj ntsuam tam sim no, peb tau siv Gao-Zi-Yaoon qub spontaneous hypertensive nas (SHR) los tshawb xyuas nws cov teebmeem kev tiv thaiv kab mob ntshav siab, thiab tshawb nrhiav cov txheej txheem uas muaj feem cuam tshuam.

Cov ntaub ntawv thiab cov txheej txheem

Kev npaj ntawm qhov ncauj tshuaj muab tshuaj

Gao-Zi-Yao tau npaj los ntawm cov khoom siv hauv qab no (Table 1) tau yuav los ntawm Suzhou Tuam Tsev Kho Mob Kho Mob (Suzhou, Tuam Tshoj) thiab tau txheeb xyuas los ntawm Duo-Rong Sheng, kws muag tshuaj ntawm cov tshuaj suav tshuaj suav tshuaj Suzhou Tuam Tsev Kho Mob Kho Mob Suav.

Txhua daim ntawv pov thawj tau muab tso rau hauv qhov chaw herbarium ntawm Suzhou Tuam Tsev Kho Mob Khomob Suav (tsis muaj tus lej tso nyiaj).

Tag nrho cov ntaub ntawv raw tau soaked rau 24 teev, thiab tom qab ntawd boiled peb zaug, condensed boiled kua peb zaug rau txog 200 mL, thiab tov nrog yaj Colla Corii Asini, Colla Carapacis thiab Plastri Testudinis kua nplaum, thiab Saccharum Granorum los ua ib tug muab tshuaj txhuam rau ntxiv daim ntawv thov.

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