Extraction Parameters Thiab Drying Methods ntawm Cistanche Deserticola

Cistanche yog Cistanche deserticola YC Ma los yogCistanche tubulosa (Schenk) Wightqhuav fleshy qia nrog scaly nplooj ntawm Orobanchiaceae nroj tsuagCistanche deserticola YC Ma[1]. Cov khoom xyaw tseem ceeb ntawm Cistanche deserticola yog phenylethanol glycosides [2-4]. Kev tshawb fawb Pharmacological tau pom tias nws muaj cov haujlwm ntawmaphrodisiac, txhim kho kev tiv thaiv, thiabtxhim khu kev nco[4-9].

Nyob rau hauv xyoo tas los no, cov txheej txheem teb saum npoo tau siv dav hauv kev rho tawm cov tshuaj suav tshuaj [{{0}}]. Txoj kev teb saum npoo av muaj qhov zoo ntawm qhov kev tshawb pom qhov tseeb, tsawg dua ntawm cov kev sim uas xav tau, kev kwv yees qhov tseeb ntawm cov qauv ua haujlwm, thiab muaj peev xwm los cuam tshuam txog kev sib raug zoo ntawm cov xwm txheej thiab kwv yees qhov tseem ceeb [15-17]. Yog li ntawd, qhov kev sim no siv cov qhab nia ntawm cov ntsiab lus ntawm tsib phenylethanol glycosides raws li cov lus teb tus nqi, thiab siv Design-Expert 8.0.6.1 software los tsim cov kev sim, optimize cov txheej txheem extraction ntawm Cistanche deserticola, thiab muab cov ntaub ntawv rau kev txheeb xyuas thiab tsim kom muaj tus qauv zoo ntawmCistanche deserticola.

Tam sim no, qhov ntsuasCheebtsam echinaceaside thiab verbascosideyog cov kev tshawb fawb kub cov ntsiab lus nyob rau hauv lub extraction thiab ua technology ntawmCistanche deserticola. Xyoo 2015 tsab ntawm Suav Pharmacopoeia tau hais tias txoj kev ua ntawm Cistanche deserticola yog "tshem tawm impurities, ntxuav, moisten, txiav rau hauv tuab slices, thiab qhuav." [1], tsis muaj kev cai meej ntawm txoj kev ziab. Yog li, qhov kev sim no tau siv yim txoj kev ziab kom qhuav, suav nrog lub hnub ziab, ntxoov ntxoo ziab, qhov cub kom qhuav (40, 60, 80, 100, 120 degree), thiab khov kom qhuav, kom ua tiav Cistanche deserticola [18], uas muab lub hauv paus theoretical rau lub Cov txheej txheem ziab ntawm Cistanche deserticola.

Ntawm kev taw qhia.

CISTANCHE EXTRACT nrog 30% ECHINCAOSIDE

1 Cov khoom siv thiab cov cuab yeej siv

Fresh Cistanche deserticola tau sau thaum Lub Kaum Hli 2018. Qhov chaw ntawm keeb kwm yog Mitan Township, Minqin County, Baiyin, Gansu Province. Associate Professor Guo Yehong ntawm Gansu Agricultural University tau txheeb xyuas tias nws yog Cistanche deserticola YC Ma, lub cev nqaij daim tawv nrog nplai nplooj. Cov khoom siv echinaceaside (batch tooj 82854-37-3, pawg pawg Ntau dua lossis sib npaug rau 98%), cistancheaside A (batch tooj 93236-42-1, pawg pawg Ntau dua lossis sib npaug rau 95%), verbascoside (batch tooj { {5}}, pawg Fraction Ntau dua lossis sib npaug li 98%), isomurabasin (batch tooj 61303-13-7, pawg pawg Ntau dua lossis sib npaug rau 98%), 2′-acetyl verbascoside (batch tooj 94492-24-7 , pawg pawg Ntau dua lossis sib npaug li 95%) tau yuav los ntawm Chengdu Pu. Feide Biotechnology Co., Ltd.; formic acid thiab methanol yog qib chromatography, Merck tuam txhab ntawm lub teb chaws Yelemees; dej yog cov dej huv huv, thiab lwm cov tshuaj reagents yog qib ntsuas.

Waters Acquity kua chromatograph, PDA detector, Empower chromatography workstation thiab quaternary hnyav tswj system; KQ-100E ultrasonic nqus tsev vacuum, Kunshan Ultrasonic Instrument Co., Ltd.

Lub tuam txhab; AL104 analytical tshuav nyiaj li cas, Mettler-Toledo Instruments Co., Ltd.; LyoQuest -85 lub tshuab ziab khaub ncaws, Telstar, Spain.

2 Cov txheej txheem thiab cov txiaj ntsig

2.1 Kev npaj cov kev daws teeb meem

Precisely ntsuas qhov tsim nyog tus nqi ntawmechinaceaside, cistancheoside A, verbascoside, isorbascoside, thiab 2'-acetyl verbascoside cov qauv siv, thiab ntxiv 70% methanol los ua kom cov concentrations ntau 1.04, 0.61, 0.63, 0.39, thiab 0.48 mg / mL ntawm kev siv tshuaj sib xyaw.

2.2 Chromatographic tej yam kev mob ntawm Cistanche deserticola

Dej Symmetry C18 (250 hli × 4.6 hli, 5 μm); kem kub 30 degree, ntim ntws 1.0 mL/min; mobile theem yog methanol -0.1% formic acid aqueous tov, elution gradient: 0 ~ 12 min, 29% ~ 34% Methanol; 12-40 min, 34% -40% methanol; 30-35 min, 40% -35% methanol; 35-40 min, 30% methanol;

1-echinacoside 2-cistanche A 3-verbascoside 4-isoacteoside 5-2′-acetylacteoside

Daim duab 1 HPLC ntawm kev siv sib xyaw (A) thiab C. deserticola qauv (B)

2.3 Kev npaj cov tshuaj ntsuam xyuas

Precisely hnyav 1 g ntawm qhuav Cistanche deserticola hmoov rau hauv ib lub hwj Erlenmeyer (dhau los ntawm ib tug 65- mesh sieve), ntxiv 50 mL ntawm 50% methanol, hnyav lub loj, ultrasonic ntawm 40 kHz ntawm 150 W, txias rau chav tsev kub. , thiab ua kom loj kom tau nws.

2.4 Kev soj ntsuam ntawm kev sib raug zoo linear

Siv {{0}}.1, {{20}}.5, 1, 2, 4, thiab 8 mL ntawm cov lus saum toj no sib xyaw ua ke rau hauv 10 mL volumetric hwj, thiab ntxiv 70% methanol rau lub cim. Precisely kos 10 μL thiab ntsuas raws li chromatographic tej yam kev mob ntawm "2.2". Qhov loj concentration yog abscissa (X) thiab lub ncov cheeb tsam yog lub ordinate (Y). Ua linear regression thiab qhov sib npaug regression yog: Echinacea Y=128 049 X -830 189, r=0.999 5, linear range 10.4 ~ 832.0 ug/mL; Cistancheside AY=87 980 -447 963, r＝0.999 8, linear range 6.3-504.0 ug/mL; Isomurabasin Y＝92 831 Glycoside Y=79 207 X-304 428, r=0999 4, kab ntau yam 4.8 ~ 384.0 ug/mL.

2.5 Methodological tshuaj xyuas

2.5.1 Precision soj ntsuam

Siv 20 μL ntawm cov tshuaj sib xyaw ua ke hauv cov khoom "2.1" thiab txhaj tshuaj 6 zaug txuas ntxiv mus ntsuas thaj chaw ncov ntawm echinaceaside, cistancheaside A, verbascoside, isorebascoside, thiab 2'-acetylverbascoside. RSDs yog 0.23% feem. , {{10}}.71%, 0.59%, 0.41%, 0.29%, qhia tias qhov ntsuas tau zoo.

2.5.2 Repeatability test

Siv 6 feem ntawm tib tus qauv Cistanche deserticola, npaj cov tshuaj ntsuam xyuas raws li cov khoom "2.3", txhaj cov qauv nyob rau hauv chromatographic tej yam kev mob ntawm yam khoom "2.2", thiab ntsuas lub ncov cheeb tsam. Cov txiaj ntsig Qhov nruab nrab loj feem ntawm echinaceaside, cistancheoside A, verbascoside, isorebascoside, thiab 2′-acetyl verbascoside yog 1.83%, 0.21%, 2.34%, 0.13%, thiab {{15 }}.68% feem, thiab RSDs yog 1.32 feem. %, 0.83%, 1.24%, 2.19%,

0.78%, qhia tias txoj kev muaj qhov rov ua tau zoo.

2.5.3 Stability test

Txiav txim siab qhov ruaj khov ntawm tib qho kev sim tshuaj (npaj raws li "2.3") ntawm 0, 2, 4, 8, 12, 24, thiab 48 teev kom tau txais echinaceaside, cistancheoside A, verbascoside, thiab isolebascoside. , 2′-acetyl verbascoside ncov cheeb tsam, RSD yog 0.58%, 0.21%, 1.58%, 1.46%, 2.19% raws li, qhia tias cov tshuaj ntsuam xyuas tau ruaj khov hauv 48 teev.

2.5.4 Sample recovery test

Noj 1 g ntawm 6 Cistanche deserticola cov qauv nrog cov ntsiab lus paub, thiab ua kom raug ntxiv ib qho ntawm cov tshuaj sib xyaw ua ke (Echinaceaside 1.06 mg / mL, Cistancheoside A 0.86 mg /mL, Verbascoside 2.17 mg/mL, Isolebascoside 0.79 mg /mL, 2′-acetyl verbascoside 0.91 mg/mL), ntxiv 70% methanol kom txog li 50 mL, thiab txhaj tshuaj raws li chromatographic tej yam kev mob ntawm "2.2". Qhov nruab nrab rov qab ntsuas yog 101.52%, 99.21%, thiab 102.62% feem. , 98.62%, 97.36%, RSD yog 1.64%, 1.95%, 0.87%, 1.59%, 1.13% raws li, thiab cov qauv rov qab tau zoo.

Cistanche yog ib hom tshuaj tonic uas paub zoo uas tuaj yeem txhawb nqa thiab tiv thaiv lub raum. Hauv cov tshuaj suav tshuaj suav tshuaj, Cistanche yog cov tshuaj ntsuab zoo tshaj plaws rau lub raum.Cistanche yog nplua nuj nyob rau hauv echinacoside, acteoside, thiab flavonoids.Cov khoom xyaw zoo hauv Cistanche tuaj yeem txo lub raum cell apoptosis thiab ua rau lub raum cell proliferation. Yog li ntawd, Cistanche yog lub raum ntuj ntxiv.