Kev Tshawb Fawb Cov Txheej Txheem Ntawm Kev Ua Ntawm Cistanche Tag Nrho Glycosides Ntawm Kab Mob Hlwb Kab Mob Raws Li Kev Siv Tshuaj Network Thiab Tsiaj Sim Ⅲ

3 Cov txiaj ntsig thiab kev tshuaj xyuas

3.1 Kev soj ntsuam ntawm Cistanche tubulosa tag nrho glycosides lub hom phiaj thiab cov hom phiaj kab mob

Import peb-dimensional cov ntaub ntawv ntawmcov khoom xyaw nquagmus rau hauv PharmMapper database kom tau txais cov ntsiab lus sib thooj ntawm cov khoom sib txawv. Cov ntaub ntawv UniProt tau siv los muab cov npe protein thiab cov ntaub ntawv noob npe sib raug rau txhua lub hom phiaj. Ua ke cov hom phiaj cov ntsiab lus sib xws rau xya lub ntsiab lus, tag nrho ntawm 354 lub hom phiaj tau txais. Hauv GeneCards database, cov noob muaj feem cuam tshuam tau tshawb fawb siv lo lus "mob plab hnyuv", thiab cov txiaj ntsig tshawb nrhiav tau txheeb xyuas, thiab 7851 lub hom phiaj tau txais thaum kawg. Siv cov xwm txheej los kos ib daim duab Venn hauv online kom tau txais kev sib tshuam ntawm cov khoom xyaw nquag ntawm Cistanche tubulosa thiab cov ntsiab lus ntawm IBD. Raws li pom hauv daim duab 1, 254 lub hom phiaj ntawm cov khoom xyaw nquag ntawmCistanche tubulosauas muaj kev tiv thaiv ntawm IBD tau txais.

Fig.2 PPI-network diagram of common targets

Fig.5 Component-target-pathway networ

Fig.7 Tag nrho glycosides ntawm Cistanche tubulosa downregulates mRNA ntawm TGF- thiab mTOR signaling pathways hauv IBD nas.

3.4 Cistanche tubulosa tag nrho glycosides txhim kho DSS-induced DAI qhab nia

Cov txiaj ntsig tau kuaj pom tau tias, tshwj tsis yog rau pawg CZ, lwm pab pawg muaj qhov sib txawv ntawm qhov hnyav poob thiab fecal occult ntshav (Daim duab 6). Ntawm lawv, pawg tswj hwm DSS muaj lub cev poob siab tshaj plaws, cov ntshav fecal hnyav tshaj plaws, DAI siab tshaj plaws, thiab pom tseeb fecal los ntshav rau hnub 6. Tom qab kev cuam tshuam ntawm Cistanche tubulosa tag nrho glycosides, DAI qhab nia maj mam txo, thiab cov ntshav occult maj mam txo. Pawg HZ muaj qhov poob qis tshaj plaws hauv lub cev thiab qhov qis tshaj ntawm cov ntshav zais hauv cov quav. Cov txiaj ntsig tau pom tias Cistanche tubulosa tag nrho glycosides txhim kho cov lus teb physiological tshwm sim los ntawm kab mob plab hnyuv.

CISTANCHE SUPPLEMENT FOR IBD TREATMENT PHGS75% ECH 30% ACT 12%

3.5 Cistanche tubulosa tag nrho glycosides down-regulate IBD

Kev nthuav qhia ntawm mTOR thiab TGF- kev taw qhia txoj hauv kev hauv nas spleen mTOR thiab TGF- kev taw qhia txoj hauv kev yog ob txoj hauv kev tseem ceeb tshaj tawm txoj hauv kev uas ua rau cov lus teb inflammatory. Kev qhia ntawm mTOR thiab TGF- hauv cov nqaij mos ntawm cov nas tau kuaj pom los ntawm qRT-PCR. Cov txiaj ntsig tau pom tias mRNA qhia ntawm mTOR thiab TGF- hauv DSS tswj pab pawg nas tau nce ntxiv piv nrog rau cov nas ib txwm muaj. Piv nrog rau DSS tswj cov nas, Piv nrog Cistanche tubulosa, kev qhia ntawm mTOR thiab TGF- hauv cov nas tau txo qis tom qab kev cuam tshuam Cistanche tubulosa. Ntawm lawv, cov pab pawg neeg noj tshuaj muaj qhov txo qis tshaj plaws, thiab qib qhia tau zoo raws li cov pab pawg ib txwm muaj (Daim duab 7). Cov txiaj ntsig tau pom tias Cistanche tubulosa tag nrho glycosides txo qis-tswj cov kev qhia ntawm mTOR thiab TGF- signaling pathways.

Fig.8 Heatmap ntawm PICRUST2-functional abundanceclustering

3.6 PICRUST2 functional annotation tsom xam

PICRUST2 yog ib qho cuab yeej bioinformatics rau kev kwv yees metagenomic muaj nuj nqi raws li kev lom neeg 16S rRNA, ASV ntoo, thiab ASV cov ntaub ntawv noob hauv Greengene database. Xaiv cov ntaub ntawv COG tau siv rau kev kwv yees ua haujlwm, thiab cov txiaj ntsig tau pom tias cov haujlwm ntawm cov hnyuv muaj feem cuam tshuam nrog kev ua haujlwm RNA; chromatin qauv thiab dynamics; cell voj voog tswj, cell faib, chromosome segmentation; tiv thaiv mechanism; signaling cell phab ntsa / cell membrane / hnab ntawv biogenesis; hlwb Nws muaj ntau tshaj 20 lub luag haujlwm xws li cov qauv tsim tawm; intracellular thauj, secretion, thiab vesicle thauj; zog tsim thiab hloov dua siab tshiab; carbohydrate thauj thiab metabolism. Xaiv 15 lub ntsiab lus tshaj plaws thiab lawv cov ntaub ntawv ntau ntxiv hauv txhua tus qauvDaim ntawv qhia tshav kub (Daim duab 8) yog kos rau cov ntaub ntawv thiab pawg los ntawm ntau qib kev ua haujlwm.

Piv nrog rau pawg CZ, pawg DZ tswj cov nas muaj COG1132 (ABC-type multidrug thauj system), COG0745 (DNA-binding teb regulator), COG1131 (ABC-type multidrug thauj system), COG1961 (Site-specific DNA recombinase ntsig txog DNA invertase Pin), COG0438 (Glycosyltransferase koom nroghauv cell phab ntsa biosynthesis), COG0534 (Na +-drivenMultidrug efflux twj tso kua mis), COG0642 (Signal transduction histidine kinase), COG1595 (DNA-directed RNA polymerase tshwj xeeb sigma subunit), COG0463 (Endonuclease), COG4974 (Site -specific recombinase XerD), COGraC-typeA muaj cov proteins), COG1136 (ABC-hom lipoprotein export system), thiab COG1309 (DNA-binding transcriptional regulator) functional gene expression abnormalities. Cov noob no feem ntau koom nrog: V: kev tiv thaiv mechanism; T: teeb liab transduction mechanism; M: cell phab ntsa / cell membrane / hnab ntawv biogenesis; I: lipid thauj thiab metabolism; K: sau ntawv; L: replication, recombination thiab kho.

Tom qab rau lub luag haujlwm no tau muab tag nrho glycosides ntawm Cistanche tubulosa, qhov kev qhia ntawm cov haujlwm sib txawv ntawm lub cev tau nyob ze rau cov pab pawg ib txwm muaj. Piv nrog rau cov nas hauv pawg CZ thiab DZ, cov nas nyob hauv pawg HZ tau muaj kev qhia siab ntawm COG1028 (Short-chain alcohol dehydrogenase family) thiab COG0451 (Nucleoside-phosphate-sugar epimerase) noob. Cov noob no muaj feem cuam tshuam rau kuv: lipid thauj thiab metabolism. Thiab M: Lub cell phab ntsa / cell membrane / lub hnab ntawv biogenesis ua haujlwm zoo sib xws. Yog li, nws tau kwv yees tias Cistanche tubulosa tag nrho glycosides tuaj yeem tswj kev puas tsuaj ntawm IBD nas los ntawm kev tswj cov lipid metabolism, qabzib metabolism thiab cuam tshuam txog kev tiv thaiv cov teeb liab hloov pauv hauv cov nas.