Txhim kho Anticancer Muaj Peev Xwm Ntawm Aspergillus Favus Endophyte Ntawm Jojoba Taxol Los Ntawm Kub Nanoparticle Conjugation Ntawm -Irradiation
May 30, 2023
Kev sib tham
Fungal endophytes nrog Taxol tsim potency tsa kev cia siab rau loj ntau lawm ntawmTaxol vim lawv txoj kev loj hlob sai, tus nqi-zoo fermentation txheej txheem, kev ywj pheej ntawm huab cuahloov, thiab muaj peev xwm ntawm genetic manipulation. Txawm li cas los xij, lubanticipation ntawm fungi rau industrialKev tsim khoom ntawm Taxol tau sib tw los ntawm lawv cov txiaj ntsig qis dua thiab poob ntawmTaxol productivity nrog subculturing [14, 16, 26, 39, 45, 53, 54]. Feem ntau ntawm endophyticTaxol-producing fungi raug cais los ntawmTaxussp. thiabPodocarpussp. uas yog koomrau tsev neeg Taxaceae [18, 39]. Tshawb nrhiav ntawm Taxol ntau lawm los ntawm endophytic fungi los ntawmnroj tsuag sab nraum tsev neeg Taxaceae nrog tej zaum Taxol productivity yog lub hom phiaj tseem ceeb los ntawmbiotechnologists.Jojobatsob nroj yog ib qho ntawm feem ntau cov kab lis kev cai lees paub cov nroj tsuag tshuajrau nws cov ethnopharmacological cuam tshuam xws li kev ua haujlwm antimicrobial, anti-inflammatory, thiabcov haujlwm tiv thaiv kabmob [24, 55]. Yog li, lub hom phiaj ntawm txoj kev tshawb fawb no yog cais tawm thiab kwv yees covTaxol tsim potency ntawm endophytic fungi los ntawm lubjojoba uacog. Ntawm cov rov qab losfungi, endophytic caisA. favmuab lub siab tshaj plaws Taxol yield (88.65 ug / l), raws li authenticatedlos ntawm TLC, UV- absorption, thiab HPLC tsom xam. Ib qho kev tshuaj ntsuam zoo sib xws rauTaxol ntau lawm tau tshaj tawm rau endophytes los ntawmGinko biloba [28] thiab lwm yam nroj tsuag[15, 18, 20, 39, 56]. Taxol yield los ntawmA. favtau pom zoo nrogP. polonicum, ib endophytes ntawmGinkgo biloba [28] thiabA. favipes [15, 39], A. terreus [15, 39], endophyte ntawmP. kev zoo siab, thiab rau endophytes ntawmTaxusua spp. xws liA. candidus, Fusarium solani [57], A. niger [58], thiabA. fumigatus. Tus kheej ntawmA. fav, Taxol muaj zog tsim cov kab mob fungalcais tawm, tau lees paub los ntawm kev tshuaj ntsuam molecular ntawm ITS cheeb tsam, thiab qhov sib lawv liag yogtso rau ntawm Genbank nrog kev nkag mus #MW485934.1, nrog rau ntawm Assiut University MycologicalCenter (AUMC), Egypt nrog deposition # AUMC13892. Ib yam li ntawd, tus kheej ntawmA. favustau lees paub raws li qhov ua ntu zus ntawm ITS cheeb tsam [49, 59], 2018, 2019, [1, 11, 36, 43, 60–68]. Cov qauv tshuaj ntawm rho tawm Taxol los ntawmA. favyog1 HNMR, thiab FT-IRkev tshuaj xyuas.
Nyem qhov no Kom Paub Cistanche Anti-cancer Functions thiab Cistanche Supplements Kev muag khoom
Cov teeb liab daws teeb meem ntawm HNMR rauA. favTaxol yeej zoo ib yam li tus qauvTaxol, uas tau faib nruab nrab ntawm 1.0 thiab 8.0 ppm. Peb lub teeb liab proton tau daws ntawm1.aromatic moieties raug daws ntawm 6.5–9.0 ppm [17, 45]. Raws li txoj cai, rau tag nrho Taxane scaffolds, signals rau lawv sab chains protons raug daws ntawm 2.0–7.0 ppm, thaum cov rau benzoate (C2), phenyl (C3), thiab benzamide (C3) pawg raug daws ntawm 7.0 thiab 8.4 ppm [69] ( [70]. FT-IR spectra ntawmA. favTaxol yog zoo li cov qhabnias Taxol, raws li coincident nrog Taxollos ntawm lwm cov kab mob fungal [3], Visalakchi et al. 2010). Cov txiaj ntsig ntawm Taxol los ntawmA. favyogoptimized los ntawm cov txheej txheem teb saum npoo siv Plakett-Burman Design [17–20, 45, 46, 49, 71]. Cov txiaj ntsig ntawm Taxol tau nce los ntawm kwv yees li 1.8 npaug ntawm cov lus teb rau cov factorialtsim khoom noj khoom haus optimization txheej txheem [17, 45]. Qhov tseem ceeb heev variables cuam tshuamTaxol production byA. favtau ntxiv optimized nrog CCD tsim, muab qhov siab tshaj plawsyield (302.72 ug/l) nrog cysteine (0.5 g/L) ntawm pH 6.0 thiab incubated rau 7 hnub. Hauv ibendeavor los txhim kho cov khoom ntawm Taxol los ntawmA. fav, cov kab mob fungal tau nthuav tawmionizing - hluav taws xob thiab cov spores tau cog rau ntawm kev hloov kho malt extract broth nruab nrab,thiab Taxol tau muab rho tawm thiab ntsuas. Thaum-rays irradiation, lub Taxol tawm los ntawmA. favus uatsis tau nce ntxiv piv rau kev tswj hwm kab lis kev cai, qhia txog qhov tsis muajInduction ntawm biosynthetic gene pawg ntawm Taxol. Txawm li cas los xij, Taxol tawm los me ntsisnce los ntawmFusarium hmoovthiabNodulisporium styliformteb rau gamma-irradiaqhov [1, 72], El-Sayed et al., 2020). Taxol productivity los ntawmA. favnyob rau hauv teb rau txawvmedia tau kawm.A. favmuab qhov siab tshaj plaws Taxol tawm los ntawm kev loj hlob ntawm Czapek's-Doxmedia thiab malt extract, raws li zoo raws li cov txiaj ntsig rau Taxol ntau lawm los ntawmA. terreus [16, 53] thiabP. polonium [28]. Txawm li cas los xij, kev nyiam ntawm PDB rau Taxol ntau lawm tau tshaj tawm rauA. candidusthiabF. solan [73].
Table 7Antimicrobial kev ua ntawm Taxol tawm tsam cov kab mob sib txawv uas sawv cev los ntawm diameter ntawm inhibition cheeb tsam (mm)
Yuav kom nce lub hom phiaj, solubility, thiab kev ua tau zoo ntawm Taxol los khi nrog lub hom phiaj proteinHauv vivo, ntau qhov kev sim ua tiav tau raug mob siab. Conjugation of AuNPs with thetsawg soluble chemotherapeutic tshuaj yog ib qho ntawm cov technologies zoo kawg nkaus tsis ntev los no kom ncesolubility thiab targetability ntawm ntau cov tshuaj chemotherapeutic. Lub purified Taxol los ntawmA. favtau conjugated nrog AuNPs, nyob rau hauv lub xub ntiag ntawm PVP raws li ib tug capping thiab stabilizingtus neeg sawv cev, kho los ntawm irradiation los ntawm-rays. Lub AuNP tau npaj los ntawm -irradiation-basedtxo qhov muaj PVP ua cov neeg ua haujlwm ruaj khov los tiv thaiv cov hlau colloids los ntawmceev aggregation, raws li qhia los ntawm cov xim hloov thiab ib tug muaj zog nqus band ntawm λ540 nm. Kev hloov xim feem ntau yog los ntawm qhov chaw plasmon resonance (SPR) [74], thiab cov ntse thiab siab-siv peaks ua kom lub siab tshaj plaws tawm los thiab loj tis ntawm lubsynthesized AuNPs. Gamma-irradiation tau raug lees paub tias yog ib qho ntawm qhov xav tau tshaj plawstxoj kev rau cov synthesis ntawm metallic nanoparticles vim lawv heev txo radicals thiabgenerating dawb electrons tsis muaj byproduct tsim [50]. Cov particle loj tau los ntawmDLS ntsuas 47.58 nm loj dua TEM cov txiaj ntsig (13.0–21.0 nm) vim DLStsom xam ntsuas lub vojvoog hydrodynamic. Los ntawm XRD tsom xam, kev txhim kho ntawmCov qauv siv lead ua ntawm Taxol-PVP-AuNPs consortium tau tshwm sim los ntawm qhov sib txawvpeaks ntawm 2ɵ=38.18 degree, 44.01 degree, 64.57 degree, 77.67 degree, thiab 81.74 degree uas piav txog Bragg qhov kev soj ntsuamntawm (111), (200), (220), (311), thiab (222), raws li [51]. Synthesized AuNPspom lub zog siv lead ua thiab muab lub ntsej muag-centered cubic (fcc) crystalline qauv, nrogib qho amorphous ncov ntawm 19.25 degree rau Taxol uas suav nrog hauv lub koom haum thiab kev ruaj khovof AuNPs [52].
Covantiproliferative kev ua ub nontawm Taxol-PVP-AuNPs conjugates raug soj ntsuam tawm tsamHEPG-2 thiab MCF-7 kab ntawm tes, normalizing rau haiv neeg Taxol thiab AuNPs, cais. Covbioactivity ntawm Taxol tau nce siab thaum sib txuas nrog AuNPs, piv raurau haiv neeg Taxol li tswj.
Qhov txhim khoanticancer kev uantawm Taxol los ntawm AuNPs tuaj yeem yog vim qhov txo qisntawm lymph kua, nce ntshav ncig thiab Taxol solubility raws liconjugation nrog AuNPs, thiab piv rau haiv neeg Taxol [75]. AuNPs tau txais txiaj ntsig zooxim vim lawv cov toxicity tsawg, ntau dua versatility los khi nrog sib txawv molecules,thiab biocompatibility [76]. Los ntawm IC50values, Taxol-PVP-AuNPs consortium disua si ib qho tseem ceeb ua si tawm tsam HEPG-2 thiab MCF-7, piv rau cov neeg Taxolthiab AuNPs cais. Hais txog bioactivity ntawm AuNPs, antiproliferativeKev ua haujlwm ntawm Taxol-PVP-AuNPs koom nrog tau nce li ntawm 4 npaug piv rauAuNPs as control. Interestingly, qhov kev ua ntawm Taxol-AuNPs consortium yog plausiblyraws li qhov tseeb Taxol. Antimicrobial kev ua haujlwm ntawmA. favTaxol-AuNPsConsortium tau raug soj ntsuam tawm tsam ntau yam kab mob pathogenic multidrug-resistant microorganisms. Thaum conjugation nrog AuNPs, cov tshuaj tiv thaiv kab mob ntawm Taxol tau muaj zog heevnce piv rau haiv neeg Taxol thiab AuNPs, cais. Taxol-AuNPS consortiumtso tawm qhov kev ua siab tshaj tawm tsamE. agglomerates, C. albicans, thiabE. coli.CovKev ua haujlwm siab dua ntawm Taxol thaum conjugation nrog AuNPs txheeb xyuas qhov bioavailability,solubility, thiab kev ua tau zoo ntawm Taxol los khi nrog lub hom phiaj tubulin protein hauv vivo.
Cov ntaub ntawv ntxiv
Qhov online version muaj cov khoom siv ntxiv muaj nyob ntawm
Kev lees paubPeb txaus siab rau kev txhawb nqa nyiaj txiag los ntawm Academy ntawm Kev Tshawb Fawb Txog Kev Tshawb Fawb thiab thev naus laus zis, Egypt, rau Prof. Ashraf SA El-Sayed. Peb ua tsaug rau Dr. Nabil Z. Mohamed, tus tswv cuab EFBL, rau nws txoj kev taw qhiaNyob rau hauv chromatographic tsom xam ntawm Taxol thiab fungal identification.
Sau Kev PabcuamAIE, MK, thiab GME conceptualize lub dav hlau tshawb fawb. SSA ua qhov kev simua haujlwm. ASE sau phau ntawv. AFE hloov kho thiab kho cov ntawv sau. Txhua tus kws sau ntawv tau nyeem thiab pom zoodaim ntawv kawg.
Nyiaj txiagQhib nkag tau nyiaj los ntawm Lub Tsev Haujlwm Saib Xyuas Kev Tshawb Fawb, Technology & Innovation Funding Authority(STDF) koom tes nrog Lub Tuam Txhab Nyiaj Txiag Egyptian (EKB). Peb txaus siab heev rau kev txhawb nqa nyiaj txiaglos ntawm Egyptian Academy of Scientific Research thiab Technology, ASRT-2021.
Cov ntaub ntawv muajTag nrho cov ntaub ntawv muaj nyob rau hauv phau ntawv no.
Kev tso cai rau kev tshaj tawmTsis siv tau
Cov ntaub ntawv
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