Cov teebmeem ntawm Cistanche Tag Nrho Glycosides Ntawm Kev Kawm Thiab Kev Tsis Txaus Siab hauv nas

Yog xav paub ntxiv:ali.ma@wecistanche.com

Gao Cong, Wang Chuanshe, Wu Guanzhong, Tu Pengfei

Abstract

Lub hom phiaj los soj ntsuam cov teebmeem ntawmCistanche tag nrho glycosidesntawm scopolamine-induced impairment ntawmkev kawm thiab kev ncothiab ntawm sodium nitrite-induced impairment ntawmkev kawm thiab kev ncokev sib sau ua ke hauv nas. Methods Kauj Ruam nqes xeem tau ua los cuam tshuam qhovkev kawm thiab kev ncontawm nas.Cistanche tag nrho glycosidestau muab tshuaj sib law liag rau 30 hnub. Cov nas tau kawm nyob rau hnub 29 thiab kev ntsuas tau ua rau hnub 30. Lub sijhawm latent thiab ntau qhov yuam kev hauv 5 min tau sau tseg. Scopolamine tau muab los ntawm kev txhaj tshuaj intraperitoneal 15 feeb ua ntej kev cob qhia, thiab sodium nitrite tau muab tshuaj subcutaneously tom qab kev cob qhia. Cov txiaj ntsig (1) Muab piv nrog cov qauv nas raug ntxias los ntawm scopolamine,cistanchetag nrhoglycosides200 thiab 400 mg / kg ua ke nrog piracetam 600mg / kg considerably nce latent lub sij hawm thiab txo lub sij hawm yuam kev.Cistanchetag nrhoglycosides100 mg / kg kuj nce lub sij hawm latent tab sis txo cov sij hawm yuam kev rau qee qhov. (2) Piv nrog rau cov qauv nas raug ntxias los ntawm sodium nitrite,cistanchetag nrhoglycosides200 thiab 400 mg / kg nce lub sij hawm latent loj tab sis tsis muaj qhov cuam tshuam loj rau lub sijhawm yuam kev.Cistanchetag nrhoglycosides100mg / kg txo lub sijhawm latent thiab lub sijhawm ua yuam kev rau qee qhov. XausCistanchistag nrhoglycosidestuaj yeem pom tseeb txhim kho scopolamine-induced impairment ntawmkev kawm thiab kev ncothiab sodium nitrite-induced impairment ntawmkev kawm thiab kev ncohauv nas.

Kev laus ntawm cov pej xeem tau ua rau kev tshawb nrhiav cov tshuaj muaj kev nyab xeeb thiab siv tau zoo rau kev tiv thaiv thiab kev kho mob Alzheimer's tus mob hotspot hauv kev tshawb fawb tshuaj. Nyob rau hauv xyoo tas los no, cov tshuaj suav tshuaj tau ua tiav zoo hauv kev kho mob Alzheimer's kab mob nrog cov tshuaj uas feem ntau ua rau lub raum thiab txhawb cov pob txha, thiab tseem ua rau lub plawv thiab tus po. Cov tshuaj suav tshuajCistanchethawj zaug luam tawm hauv "Shen Nong's Materia Medica". Nws muaj cov teebmeem ntawm tonifying raum yang, nourishing essence thiab ntshav, thiab moistening cov hnyuv thiab laxatives. Nws yog tam sim no dav siv nyob rau hauv kev kho mob ntawm senile dementia, impotence, ib txwm cem quav, thiab lwm yam kab mob. Antioxidant, tiv thaiv hypoxia, tiv thaiv hluav taws xob, thiab kev tiv thaiv kev ua haujlwm zoo; Lub ntsiab tivthaiv yog phenylethanoid glycosides.Cistanche tag nrho glycosidesyog muab rho tawm los ntawm qhuav fleshy stems ntawm cistanche tubulosa, muaj 90.7 feem pua ​​ntawmphenylethanoid glycosides thiab 44.3 feem pua ​​​​ntawm echinacoside, cov khoom tseem ceeb. Hauv daim ntawv no, cov teebmeem ntawmcistanche tag nrho glycosidesntawmkev kawm thiab kev ncoKev puas tsuaj tshwm sim los ntawm scopolamine thiab sodium nitrite hauv nas tau kawm, thiab lub hauv paus rau lawv txoj kev loj hlob raws li cov tshuaj tshiab rau nootropic thiab kev tiv thaiv thiab kev kho mob ntawm senile dementia tau muab.

Nyem rau Cistanche qia rau kev nco

1 Cov khoom siv thiab cov txheej txheem

1.1 Tsiaj

180 noj qab nyob zoo Kunming nas, hnyav 18-22 g, ib nrab txiv neej thiab ib nrab txiv neej, tau muab los ntawm Tsiaj Center ntawm Tuam Tshoj Pharmaceutical University, thiab daim ntawv pov thawj naj npawb yog Sudong (Quality) 97004.

1.2 Tshuaj thiab Reagents

Cistanche tag nrho glycosides, muab los ntawm Chemistry Office of the Modern Research Center of Traditional Chinese Medicine, Peking University, batch naj npawb 980601. Naofukang ntsiav tshuaj, tsim los ntawm Yixing Pharmaceutical Factory, batch tooj 980521. Scopolamine txhaj yog tsim los ntawm Shanghai Hefeng Pharmaceutical Co., Ltd., batch naj npawb 980101. Sodium nitrite, tsim los ntawm Rugao Huarui Reagent Factory, batch naj npawb 980220. Cov tshuaj uas tau hais los saum toj no thiab cov tshuaj reagents tau npaj nrog dej distilled rau qhov concentration xav tau rau kev sim ua ntej siv.

2 Kev tshwm sim

2.1 Cov teebmeem ntawm scopolamine-inducedkev kawm thiab kev ncoKev tsis txaus ntseeg ntawm cov nas Piv nrog rau cov pab pawg ib txwm muaj, qhov kev sim latency ntawm lub platform kuaj hauv pawg qauv tau luv luv thiab cov lej yuam kev tau nce ntxiv, qhia tias qhovkev kawm thiab kev ncokev nrhiav tau ntawm nas yog impaired. Piv nrog rau pawg qauv, qhov kev xeem latency ntawm pawg Naofukang thiab covcistancheCov koob tshuaj ntau thiab cov koob tshuaj nruab nrab tau ntev heev, thiab cov lej ntawm cov kev ua yuam kev tau txo qis (P<0.01); the="" test="" latency="" of="" the="">cistanchepab pawg uas tsis muaj koob tshuaj kuj tseem ua tau ntev heev (P<0.01). );="" the="" number="" of="" test="" errors="" decreased="" to="" a="" certain="" extent,="" but="" there="" was="" no="" significant="" difference="" compared="" with="" the="" model="" group.="" the="" results="" showed="" that="">cistanche tag nrho glycosidestau txhim kho scopolamine-inducedkev kawm thiab kev ncokev puas tsuaj rau cov nas, thiab cov nyhuv ntawm cov pab pawg siab thiab nruab nrab koob tau zoo dua li ntawm pawg Naofukang. Cov txiaj ntsig tau pom nyob rau hauv Table 1.

Table 1 Qhov cuam tshuam ntawm cistanche tag nrho glycosides ntawm scopolamine-vim kev kawm thiab nco tau qhov tsis zoo hauv nas (x±s)

Nco tseg:Piv nrog rau pawg qauv, * P<0.05,><0.01. in="" (="" )="" is="" the="" number="" of="" deaths="" by="" electric="" shock,="" the="" same="">

2.2 Cov teebmeem ntawm sodium nitrite raukev kawm thiab kev ncoconsolidation disorders nyob rau hauv nas Qhov kev ntsuam xyuas latency ntawm lub platform xeem nyob rau hauv cov qauv pab pawg neeg yog luv luv thiab tus naj npawb ntawm cov xeem yuam kev nce. Piv nrog rau cov pab pawg ib txwm, qhov sib txawv yog qhov tseem ceeb, qhia tias qhovkev kawm thiab kev ncoconsolidation ntawm cov qauv pab pawg neeg yog impaired. Piv nrog rau cov qauv pab pawg, qhov kev xeem latency ntawm nas nyob rau hauv cov pab pawg neeg siab thiab nruab nrab koob ntawmcistanchetau ntev heev (P<0.05-0.01); the="" number="" of="" errors="" decreased="" to="" a="" certain="" extent,="" but="" there="" was="" no="" statistical="" significance;="" the="" test="" latency="" and="" errors="" of="" the="" mice="" in="" the="" low="" dose="" group="" of="">cistancheTus naj npawb ntawm cov sij hawm kuj tau txhim kho rau qee qhov, tab sis tsis muaj qhov tseem ceeb ntawm kev txheeb cais; Qhov ntsuas latency ntawm cov nas nyob rau hauv pab pawg Naofukang tau ntev heev, thiab cov lej ntawm kev xeem raug txo qis (P<0.01). the="" results="" of="" this="" experiment="" showed="" that="" oral="" administration="" of="" high="" and="" medium="" doses="" of="">cistanche tag nrho glycosidestau pom tseeb txhim kho cov teebmeem ntawmkev kawm thiab kev ncoconsolidation disorder nyob rau hauv nas induced los ntawm sodium nitrite, tab sis nws tsis muaj zog tshaj Naofukang. Cov txiaj ntsig tau qhia hauv Table 2.

Table 2 Qhov cuam tshuam ntawm Cistanche tag nrho glycosides ntawm kev kawm thiab kev nco ua kom tsis muaj zog hauv cov nas vim yog sodium nitrite (x ± s)

3 Kev sib tham

Kev kawm thiab kev ncoyog cov ua haujlwm siab heev ntawm lub hlwb thiab cov ntsiab lus uas tsim kev txawj ntse. Tag nrho cov haujlwm neural ntawm kev kawm thiab kev nco, nws tuaj yeem muab faib ua peb yam txheej txheem neural: nrhiav tau, sib sau ua ke thiab kev tsim tawm. Kev kawm thiab kev nco tsis zoo yog ib qho tsos mob ntawm ntau yam ntawm encephalopathy, nrog rau cov hlab ntsha hauv hlwb hauv cov laus, cov ntshav tsis txaus, thiab dementia.

Cov tshuaj suav tshuaj ntseeg hais tias Alzheimer's kab mob thiab lwm yam kab mob feem ntau yog deficiency syndromes.Cistancheyog ib qho tshuaj tonic thiab tshuaj aphrodisiac, uas muaj lub luag haujlwm ntawm invigorating lub raum, sau cov ntsiab lus thiab moistening cov hnyuv. "Ben Cao Jing Shu" hu nws yog ib qho tshuaj tseem ceeb rau kev noj qab haus huv thiab cov ntshav thiab txhawb lub raum. Nws muaj txiaj ntsig zoo rau qaug zog, tsis nco qab, tsis hnov ​​​​lus thiab enuresis tshwm sim los ntawm lub raum tsis txaus. zoo curative nyhuv. Zhu Zhiming et al. txheeb xyuas cov tshuaj muaj pes tsawg leeg ntawm 46 cov ntawv sau tshuaj rau kev kho mob senile dementia luam tawm hauv cov ntawv xov xwm cuam tshuam los ntawm 1990 txog 1999 thiab pom tiasCistanchedeserticola yog ib qho ntawm cov tshuaj siv ntau tshaj 1. Kev sim tsiaj los ntawm Sato Zhou hauv Nyij Pooj tau lees paub tiascistancheNws muaj cov nyhuv anti-dementia131. Peb qhov kev sim yav dhau los tau pom tiascistanche tag nrho glycosidesmuaj kev txhawb nqa tseem ceeb rau covkev kawm thiab kev ncokev ua haujlwm ntawm cov nas ib txwm muaj, thiab muaj txiaj ntsig zoo rau ethanol-inducedkev kawm thiab kev ncoreproduction disorder qauv nas, raum-yang deficiency qauv nas, vascular dementia qauv nas, thiab lwm yam. Kev kawm thiab kev nco muaj nuj nqi muaj kev tiv thaiv pom tseeb. Ua ke nrog cov txiaj ntsig ntawm qhov kev sim no, nws tau lees paub tiascistanchemuaj cov teebmeem nootropic, thiab muaj kev tiv thaiv pom tseeb ntawm kev nrhiav tau, sib sau ua ke thiab kev tsim tawm ntawm peb cov txheej txheem neural kev ua haujlwm hauv tag nrho cov haujlwm neural ntawmkev kawm thiab kev nco, qhia tias cov teebmeem nootropic ntawmcistanche tag nrho glycosidesmuaj ntau yam Hierarchical thiab multi-segment. Yog li ntawd,cistanche tag nrho glycosidestuaj yeem dhau los ua cov tshuaj zoo rau kev kho mob ntawm senile dementia thiab lwm yam kab mob nrogkev kawm thiab kev ncokev puas tsuaj raws li cov tsos mob tseem ceeb.

Cov ntaub ntawv

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