Cov txiaj ntsig ntawm Kev Sib Txuas Kev Kho Mob Ntawm Laminaria Japonica Thiab Cistanche Tubulosa Extracts hauv Kev Loj Hlob
Mar 03, 2022
Kyungha Shin1#, Tae-Su Kim2#, Jangbeen Kyung1, Dajeong Kim1, Dongsun Park1, Ehn-Kyoung Choi1, Sung-Pyo Lee2, Woong-Suk Yang2, Myung-Hwa Kang3, Yun-Bae Kim1*
1College of Veterinary Medicine, Chungbuk National University, Cheongju, Kauslim
2 Misuba RTech Co., Ltd., Asan, Kauslim Teb
3 Department of Food Science thiab Nutrition, Hoseo University, Asan, Kauslim
Txij li thaum tawv taub hau plaub hau tau nce tsis ntev los no txawm nyob rau hauv cov tub ntxhais hluas, cuam tshuam rau tib neeg lub neej zoo, cov plaub hau kev loj hlob-stimulating los ntawm Laminaria japonica extract (LJE) thiabCistanche tubulosaExtract (CTE) tau tshawb xyuas. Tom qab lees paub qhov anagen theem ntawm cov hauv paus hniav hauv qab shaving, txiv neej C57BL / 6 nas tau dermally thov nrog 3 feem pua Minoxidil los yog siv qhov ncauj nrog kev sib txuas ntawm LJE thiab CTE rau 21 hnub. Minoxidil txhawb cov plaub hau regrowth thiab nce -glutamyl transpeptidase (-GTP) thiab alkaline phosphatase (ALP) kev ua ub no. Tsis tas li ntawd, Minoxidil up-regulated epidermal growth factor (EGF) thiab vascular endothelial growth factor (VEGF) ntau ntau. Co-administration ntawm LJE thiab CTE ntawm 54 mg / kg LJE ntxiv rau 162 mg / kg CTE exerted synergistic txhawb cov teebmeem ntawm cov plaub hau regrowth, piv rau 3 feem pua Minoxidil. LJE nyiam txhim kho ALP kev ua si, thaum CTE nce ob qho tib si -GTP thiab ALP kev ua ub no nrog rau EGF thiab VEGF kab lus. Hauv vivo cua hnab ntim cov qauv, carrageenan-vim vascular exudation, thiab nce nitric oxide thiab prostaglandin E2 concentrations hauv cov exudates tau synergistically suppressed los ntawm kev tswj hwm ntawm LJE thiab CTE. Tsis tas li ntawd, inflammatory cell infiltration tau cuam tshuam los ntawm kev sib xyaw ua ke. Cov txiaj ntsig tau qhia tias kev kho qhov ncauj sib xyaw nrog LJE thiab CTE hauv cov koob tshuaj tsim nyog thiab qhov sib piv tiv thaiv cov plaub hau tsis zoo thiab txhim kho alopecia, uas tej zaum yuav yog ib feem kho los ntawm lawv cov kev ua ub no.
Ntsiab lus:Cov plaub hau loj hlob, o, Laminaria japonica,Tubulosa,kev kho mob ua ke
Yog xav paub ntxiv thov hu rau:Joanna.jia@wecistanche.com
Cistanchedeserticola muaj ntau yam teebmeem, nyem qhov no kom paub ntau ntxiv
Tsis ntev los no, tawv taub hau cov plaub hau tau nce ntxiv txawm nyob rau hauv cov tub ntxhais hluas, cuam tshuam rau tus neeg lub neej zoo. Txawm hais tias baldness tsis yog ib qho teeb meem ntawm kev noj qab haus huv, nws yuav ua rau muaj kev puas siab puas ntsws-tsis xis nyob hauv zej zog. Yog li ntawd, nws yog qhov txaus siab los tsim cov kev tiv thaiv tshiab thiab / lossis cov ntaub ntawv kho mob rau cov plaub hau poob thiab regrowth. Txawm hais tias kev lag luam billions nyiaj, muaj ob peb yam khoom siv tam sim no muaj [1]. Cov hauv paus plaub hau hloov kho lawv tus kheej thaum lub sij hawm cycling ntawm kev loj hlob (anagen), regression (catagen), so (telogen), thiab shedding (exogen) [2]. Thaum lub sij hawm catagen,
Feem ntau ntawm cov follicular hlwb tau raug programmed cell tuag (apoptosis) [3], txo cov follicle loj raws li cov follicles nkag mus rau telogen. Kev ua kom tsis tshua muaj-cycling epithelial qia hlwb nyob rau hauv cheeb tsam bulge ntawm cov follicles ua rau follicular regeneration ntawm qhov pib ntawm theem tom ntej anagen [4]. Stem cell progeny tsim ib tug tshiab follicular matrix thaum ntxov anagen, thiab cov plaub hau ncej thiab puab hauv paus sheath yog muab los ntawm cov kuj- undifferentiated matrix hlwb [5]. Nws yog qhov paub zoo tias qhov loj ntawm cov hauv paus plaub hau thiab lub sijhawm ntawm anagen theem tswj qhov loj (inch) thiab ntev ntawm cov plaub hau ncej, feem. Qhov no cyclic kev loj hlob ntawm cov plaub hau yog tswj los ntawm ntau yam kev loj hlob yam.
Minoxidil, finasteride, thiab propecia yog cov lag luam muag kom qeeb cov plaub hau thiab pab txhawb cov plaub hau regrowth. Minoxidil ua cov tshuaj vasodilator tau ua rau muaj kev cuam tshuam los pab txhawb cov plaub hau kev loj hlob [6] uas tej zaum yuav yog vim nws cov khoom noj khoom haus thiab cov poov tshuaj channel-qhib cov dej num [7,8]. Minoxidil muaj txiaj ntsig zoo rau androgenic alopecia, ntawm lwm cov kev kho mob baldness, txawm hais tias nws cov txiaj ntsig zoo ploj hauv lub hlis tom qab txiav kev kho mob. Los ntawm kev sib piv, finasteride thiab propecia, kev kho mob ntawm benign prostatic hyperplasia thiab prostate cancer, tau sau npe nyob rau hauv ntau lub teb chaws rau androgenetic alopecia (txiv neej-pattern baldness). Finasteride thiab propecia yog hluavtaws los tiv thaiv androgens, inhibiting hom II 5- reductase, ib qho enzyme uas hloov testosterone rau dihydrotestosterone (DHT) hauv papilla hlwb hauv qab ntawm cov hauv paus plaub hau [9]. Laminaria japonica, Undaria pinnatifida, thiab Cladosiphon okamuranus, dav faib nyob rau hauv ntau lub teb chaws, tau siv raws li kev kho mob nyob rau hauv Oriental tshuaj rau lub sij hawm ntev. Hauv kev tshawb fawb yav dhau los, fucoidan, sulfate polysaccharide complex, tau muab rho tawm thiab txheeb xyuas los ntawm cov seaweeds thiab paub tseeb tias muaj cov tshuaj tiv thaiv oxidative, tiv thaiv coagulative, tiv thaiv qog noj ntshav, thiab tiv thaiv kev ua haujlwm [10,11]. Tom qab ntawd, cov txiaj ntsig zoo ntawm fucoidan rau cov kab mob inflammatory, ischemia, lub cev tsis muaj zog, thiab cov qog tau tshwm sim [12,13]. Ntawm qhov tod tes,Cistanche tubulosa extract(CTE) kuj tau siv dav rau cov tshuaj ib txwm siv hauv Suav teb. CTE txhim kho cov ntshav khiav los ntawm kev txo cov roj cholesterol hauv cov ntshav [14]. Tsis ntev los no, nws tau tshaj tawm tias CTE txo qis kev tsim cov qog-necrosis factor- (TNF- ) thiab interleukin -4 (IL-4) uas yog yam tseem ceeb rau nitric oxide (NO) ntau lawm hauv txoj kev inflammatory [15]. Peb kuj tau pom tias fucoidan los ntawm Laminaria japonica extract (LJE) thiab CTE synergistically attenuated o hauv vitro thiab hauv vivo [16]. Qhov tseem ceeb, nws tau pom tias muaj kev sib raug zoo ntawm daim tawv nqaij (tshwj xeeb tshaj yog lacrimal caj pas) o nrog alopecia [17]. Yog li, cov tshuaj tiv thaiv kab mob ntawm kev kho alopecia tau tshwm sim [18]. Raws li kev txhim kho kev ua haujlwm ntawm cov ntshav khiav thiab mob, peb tshawb xyuas qhov kev ua tau zoo ntawm LJE thiab CTE ntawm cov plaub hau kev loj hlob thiab cov txheej txheem hauv qab, piv nrog 3 feem pua Minoxidil (Hyundai Pharmaceutical, Seoul, Kauslim), cov plaub hau zoo. kev loj hlob ntxiv.
CistancheExtract Anti-Hair Loss
Cov ntaub ntawv thiab Cov txheej txheem
Khoom siv
LJE thiab CTE tau txais los ntawm Misuba RTech Co., Ltd. (Asan, Kauslim). Cov hmoov qhuav ntawm Laminaria japonica tau muab rho tawm hauv dej huv ntawm 60oC rau 24 teev, lim los ntawm 0.45 µm daim nyias nyias, tshuaj tua kab mob, thiab tshuaj tsuag kom qhuav. Cistanche tubulosa tau muab rho tawm hauv 80 feem pua ethanol, tshuaj tua kab mob, concentrated, thiab qhuav. LJE thiab CTE tau khaws cia ntawm 4oC, sib tov (1: 3) thiab yaj hauv dej huv ua ntej siv, thiab muab tshuaj rau hauv qhov ntim ntawm 5 mL / kg.
Tsiaj txhu
Txiv neej C57BL/6 nas rau kev kawm txog kev loj hlob ntawm cov plaub hau thiab txiv neej ICR nas rau kev kawm mob tau yuav los ntawm Daehan Biolink (Eumseong, Kauslim), thiab nyob hauv ib chav nrog ib puag ncig ib puag ncig (23 ± 2ºC; 45-65 feem pua ntawm cov av noo. ; 12- teev lub teeb-tsaus voj voog; 150-300 lux brightness). Pellet pub thiab dej huv muaj nyob rau hauv libitum. Txhua qhov kev sim tsiaj tau pom zoo los ntawm Pawg Saib Xyuas Tsiaj Tsiaj thiab Siv (IAACUC) ntawm Chungbuk National University (CBNU), Kaus Lim Kauslim, thiab ua raws li Cov Txheej Txheem Kev Ua Haujlwm (SOP) ntawm Laboratory Tsiaj Tshawb Fawb Center, CBNU.
Kev kho rau cov plaub hau loj hlob
Tom qab lees paub lub anagen theem los ntawm kev soj ntsuam homo-geneously-daj xim ntawm daim tawv nqaij nyob rau hauv daim tawv nqaij tom qab ntawm C57BL/6 nas (6 lub lis piam, n=8/pab pawg) tom qab clipping nyob rau hauv lub telogen theem, cov plaub hau tau shaved [1 ,19,20] ib. Txij hnub tom qab, cov nas uas tsis pom qhov khawb tau raug xaiv thiab kho qhov ncauj nrog LJE (18 lossis 54 mg / kg), CTE (54 lossis 162 mg / kg), lossis lawv qhov sib xyaw (18 ntxiv 54 lossis 54 ntxiv 162 mg / kg) ib hnub rau 21 hnub. Cov koob tshuaj tsawg ntawm LJE (18 mg / kg) thiab CTE (54 mg / kg) yog los ntawm kev kwv yees tib neeg koob tshuaj ntawm 100 mg / lub cev (70 kg) thiab 300 mg / lub cev tom qab qhov chaw txhais ntawm lub cev hnyav (Km{{23}) } rau human/Km=3 rau nas), feem. Cov koob tshuaj ntau ntawm LJE thiab CTE tau teeb tsa ntawm 3 folds, ib leeg lossis ua ke, txhawm rau ntsuas qhov muaj txiaj ntsig zoo tshaj plaws [16]. Minoxidil (3 feem pua , 0.2 mL) tau pleev xim rau ib hnub rau ntawm daim tawv nqaij shaved tib lub sijhawm nrog kev kho LJE / CTE.
Kev soj ntsuam tag nrho ntawm cov plaub hau loj hlob
Hnub ntawm 3-hnub ncua sij hawm tom qab chais plaub hau thiab thaum lub sij hawm kho, cov nas tsuag tau maj mam muab tshuaj loog nrog diethyl ether, thiab cov duab raug coj los ntawm lawv sab nraub qaum. Qhov piv ntawm cov plaub hau regrowth (regrown cheeb tsam / shaved cheeb tsam) ntawm cov duab tau soj ntsuam siv computerized duab tsom xam system (Imageinside, Daejeon, Kauslim).
Microscopic kuaj ntawm cov hauv paus plaub hau
Hnub tim 14 thiab 21, cov nas tau muab txi los ntawm kev sib sib zog nqus tshuaj loog nrog diethyl ether, thiab lawv cov tawv nqaij dorsal raug tshem tawm [1]. Cov tawv nqaij dorsal tau txiav rau hauv ob feem. Formalin-taw, paraffin-embedded cov ntaub so ntswg (5 µm inch) ntawm ib feem tau stained nrog hematoxylin-eosin los tshuaj xyuas cov hauv paus plaub hau thiab cov kev tshawb pom histological. Lwm qhov ntawm cov ntaub so ntswg tau khov rau hauv cov kua nitrogen rau kev soj ntsuam enzyme thiab immunoassay.
Kev soj ntsuam ntawm kev ua haujlwm enzyme
Cov ntaub so ntswg ntawm daim tawv nqaij yog homogenized nyob rau hauv 4 ntim ntawm phosphate tsis (pH 7.4). Lub homogenate tau centrifuged ntawm 3, 000 rpm rau 20 min, thiab cov supernatant tau siv rau kev soj ntsuam ntawm -glutamyl transpeptidase (-GTP) thiab alkaline phosphatase (ALP) cov dej num siv p-nitrophenol phosphate (PNPP)-DEAV raws li substrate [1,21].
Quantification ntawm kev loj hlob yam
Epidermal growth factor (EGF) thiab vascular endothelial growth factor (VEGF) tau txheeb xyuas siv cov khoom siv enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Minneapolis, USA) raws li cov chaw tsim khoom cov lus qhia [1,22].
Kev kho mob rau o
ICR nas (7 lub lis piam, n{1}} / pab pawg) tau kho qhov ncauj nrog LJE (18 lossis 54 mg / kg), CTE (54 lossis 162 mg / kg), lossis lawv qhov sib xyaw (18 ntxiv 54 lossis 54 ntxiv 162 mg / kg) ib hnub ib zaug rau 7 hnub [16]. Hnub 1 ntawm LJE thiab CTE kev kho mob, cov nas tau txhaj tshuaj subcutaneously nrog 10 mL ntawm cov cua tsis muaj menyuam mus rau sab nraub qaum los tsim ib lub hnab [16,23,24]. Tom qab 2 thiab 5 hnub, lub hnab tau rov txhaj tshuaj nrog 5 mL ntawm huab cua. Ib teev tom qab kev kho LJE thiab CTE zaum kawg, 1 mL ntawm carrageenan (1 feem pua hauv saline; Sigma-Aldrich, St. Louis, USA) lossis nws lub tsheb (saline) tau txhaj rau hauv hnab.
Analysis ntawm exudates
Lub hnab cua tau ntxuav nrog 1 mL ntawm cov kua qaub txias 6 teev tom qab txhaj tshuaj carrageenan, thiab cov ntim ntim.
Cov kua dej lavage tau sau tseg [16] Tag nrho cov naj npawb ntawm cov kab mob inflammatory xws li neutrophils, monocytes, thiab lymphocytes tau txiav txim siab siv lub txee colter. Qhov siab ntawm NO thiab prostaglandin E2 (PGE2) tau txiav txim siab los ntawm Griess reagent (Sigma-Aldrich) thiab
enzyme immunoassay (EIA) siv cov khoom siv Correlate-EIA (Assay Designs, Ann Arbor, USA), feem [16].
Cistancheextract los tiv thaiv kev laus thiab tiv thaiv plaub hau poob
Kev txheeb cais
Cov ntaub ntawv raug nthuav tawm raws li qhov ntsuas ± tus qauv yuam kev. Qhov tseem ceeb ntawm cov ntaub ntawv tau txiav txim los ntawm ib txoj kev tsom xam ntawm qhov sib txawv, ua raws li posthoc Tukey qhov kev sib piv ntau yam. P-tus nqi<0.05 were="" considered="" to="" be="" statistically="">
Cov txiaj ntsig
Daim ntawv thov ntawm Minoxidil (3 feem pua) , cov ntaub ntawv siv, tau ua kom cov plaub hau loj hlob ntawm C57BL / 6 nas, nthuav tawm qhov ze-tag nrho (92.4 feem pua) ntawm thaj chaw shaved (Figures 1, 2B) piv rau 29.2 feem pua ntawm qhov qub. kev loj hlob (Daim duab 1, 2A) nyob rau hnub 21. Txawm hais tias tsis tshua muaj txiaj ntsig zoo dua li Minoxidil daim ntawv thov, kev kho qhov ncauj ntawm LJE kuj pab txhawb cov plaub hau kev loj hlob, ua rau 33.5 thiab 69.1 feem pua ntawm cov kev pab cuam ntawm 18 thiab 54 mg / kg, raws li (Daim duab 1, 2C, 2D). CTE kuj tseem muaj txiaj ntsig zoo hauv kev txhim kho cov plaub hau kev loj hlob, nce mus txog 73.6 thiab 80.4 feem pua
kev pab them nqi ntawm 54 thiab 162 mg / kg, raws li (Daim duab 1, 2E, 2F). Qhov tseem ceeb, kev sib xyaw ua ke nrog kev siv tshuaj ntau ntawm LJE (54 mg / kg) thiab CTE (162 mg / kg) zoo kawg nkaus txhim kho cov plaub hau kev loj hlob txij hnub 12, piv rau cov nyhuv ntawm Minoxidil (Daim duab 1, 2H), txawm tias cov koob tshuaj LJE tsawg. (18 mg / kg) thiab CTE (54 mg / kg) ua kom muaj kev sib koom ua ke tsuas yog thaum ntxov (Figures 1, 2G).
Piv nrog rau cov hauv paus plaub hau ib txwm muaj (Daim duab 3A), Minoxidil daim ntawv thov txhim kho kev ncaj ncees ntawm cov hauv paus plaub hau, qhia cov hauv paus loj thiab cov plaub hau tuab dua (Daim duab 3B). Cov teebmeem zoo li no kuj pom muaj nyob rau hauv daim tawv nqaij ntawm nas kho nrog LJE thiab/los yog CTE (Figures 3C-3H). Tshwj xeeb, cov plaub hau follicle-strengthening kev ua tau zoo ntawm kev sib xyaw ua ke ntawm LJE thiab CTE yog piv rau Minoxidil.
Minoxidil daim ntawv thov nce kev ua haujlwm ntawm -GTP uas ua rau cov hauv paus plaub hau mus rau theem anagen los ntawm 90-120 feem pua
nyob rau hnub 14 thiab 21, piv rau theem ntawm daim tawv nqaij ib txwm (Daim duab 4). LJE (54 mg / kg) kuj tau txhim kho cov haujlwm enzyme, txawm hais tias muaj kev hloov pauv siab. CTE tau txais txiaj ntsig zoo sib xws, ua kom muaj txiaj ntsig zoo - GTP kev ua haujlwm rau hnub 14. Qhov zoo siab, kev sib xyaw ua ke ntawm LJE thiab CTE ntxiv cov enzyme kev ua haujlwm siab dua li thaum cov khoom siv rho tawm tau siv ib leeg.
Kev ua ALP, ib qho cim ntawm cov pob txha loj hlob thiab angiogenesis, nce ntau hauv daim tawv nqaij hnub 14 thiab 21 tom qab siv tshuaj pleev Minoxidil (Daim duab 5). LJE thiab CTE kuj tau nce ALP kev ua haujlwm tom qab kev tswj hwm qhov ncauj ntawm kev noj tshuaj. Qhov tseem ceeb, kev ua haujlwm enzyme tau sib koom ua ke los ntawm kev kho mob hauv tebchaws nrog LJE thiab CTE.
Hauv kev tshuaj xyuas ntawm EGF, qhov paub zoo ntawm cov plaub hau kev loj hlob, Minoxidil daim ntawv thov txhim kho qib ntawm EGF
3.5 folds ntawm kev tswj ib txwm nyob rau ob hnub 14 thiab 21 (Daim duab 6). LJE (18 mg / kg) nce EGF nkaus xwb nyob rau hnub 21, thaum CTE (162 mg / kg) zoo heev up-regulated nws cov khoom nyob rau hnub 14 thiab 21. Nws yog qhov txaus siab rau nco ntsoov tias kev sib xyaw ua ke ntawm LJE thiab CTE txawm nyob ntawm koob tshuaj tsawg tau pom muaj kev sib koom ua ke.
Kev kho mob Minoxidil kuj nce qib ntawm VEGF los ntawm 50-80 feem pua ntawm cov nqi tswj (Daim duab 7). Txawm hais tias LJE tsis muaj txiaj ntsig, CTE tau ua kom muaj txiaj ntsig zoo tshaj plaws. Tsis tas li ntawd, kev sib xyaw ua ke ntawm LJE thiab CTE tau nthuav tawm kev ua haujlwm sib koom ua ke ntawm theem pib ntawm hnub 14.
Txhaj tshuaj carrageenan rau hauv ICR nas lub hnab cua tau nce lub ntim ntim hauv lub hnab 6 npaug ntawm qib tswj (Table 1). Xws li exudation tau txo qis los ntawm kev kho nrog LJE (18 thiab 54 mg / kg) lossis CTE (54 thiab 162 mg / kg). Qhov tshwj xeeb, kev kho mob sib xyaw hauv tebchaws nrog LJE thiab CTE tau txo qis ntxiv
lub exudate ntim, piv rau tus kheej cov teebmeem. Tus naj npawb ntawm cov kab mob inflammatory tau nce zuj zus los ntawm kev txhaj tshuaj carrageenan: 34.6, 4.2, thiab 24.9 folds tswj qib rau neutrophils, monocytes, thiab lymphocytes, raws li (Table 1). Txawm li cas los xij, LJE thiab CTE txo qis cov kab mob inflammatory hauv cov koob tshuaj, uas LJE zoo dua rau CTE. Cia siab tias, cov teebmeem kev sib koom ua ke hauv cov kab mob inflammatory cell infiltration tau txais los ntawm kev koom tes ntawm LJE thiab CTE.
Nyob rau tib lub sijhawm nrog cov kab mob ntawm tes infiltration hauv lub hnab, TSIS TAU thiab PGE2 cov ntsiab lus hauv lub hnab kuj tau nce ntxiv tom qab txhaj tshuaj carrageenan (Table 1). Hauv kev sib piv nrog cov nyhuv me me ntawm LJE,
CTE markedly txo qhov tsis muaj concentration. Combi- teb chaws kev kho mob ntawm LJE thiab CTE ntxiv txo qis NO theem nyob rau hauv ib koob tshuaj. Ib yam li ntawd, qhov nce qib PGE2 tau sib koom ua ke
suppressed los ntawm kev sib xyaw ua ke ntawm LJE thiab CTE (Table 1).
Cistancheextract txhim khotiv thaiv kab mobthiab tiv thaiv plaub hau poob
Kev sib tham
Tib neeg lub taub hau muaj qhov nruab nrab ntawm 100,000 cov hauv paus plaub hau.
Thaum lub sij hawm lub neej, txhua lub hauv paus loj hlob thiab tawm txog 20 zaug, qhia cov plaub hau txhua hnub ntawm 100 strands [25]. Cov plaub hau poob nrog rau alopecia thiab baldness yog ib qho kev nyuaj siab rau cov txiv neej thiab poj niam, tshwj xeeb tshaj yog nyob rau hauv niaj hnub haiv neeg. Yog li ntawd, nws yog ib qho tseem ceeb heev rau kev tsim kho tshiab uas tiv thaiv cov plaub hau tsis zoo thiab txhim khu kev kho plaub hau; Piv txwv li, vascular exudation thiab inflammatory cell infiltration tau synergistically suppressed. Hauv kev txheeb xyuas ntau, cov kws kho mob loj NO thiab PGE2 tau tswj hwm tau zoo tom qab kev tswj hwm qhov ncauj ntawm LJE thiab CTE. Interestingly, txij li thaum LJE tshwm sim los tiv thaiv kev ua haujlwm siab dua li ntawm ib nrab purified fucoidan [16], nws xav tias tej zaum yuav muaj cov khoom xyaw ntxiv, ntxiv rau fucoidan, hauv LJE.
Ua ke, cov txiaj ntsig tau qhia tias kev kho qhov ncauj ua ke nrog LJE thiab CTE txhim kho cov plaub hau poob los ntawm kev tswj hwm kev loj hlob thiab cuam tshuam txog kev ua haujlwm enzyme thiab ib feem ntawm kev tiv thaiv kab mob. Yog li, nws tau pom zoo tias kev sib xyaw ua ke suav nrog LJE thiab CTE hauv cov tshuaj kho mob tsim nyog thiab qhov sib piv yuav yog ib daim ntawv qhia neeg sib tw rau cov neeg mob plaub hau.
Kev lees paub
Qhov kev tshawb fawb no tau txais kev txhawb nqa nyiaj txiag los ntawm Kev Lag Luam Kev Lag Luam Kev Lag Luam rau Kev Lag Luam Hauv Cheeb Tsam ntawm Chungcheong Leading Industry Office, Kaus Lim Kauslim Lub Tsev Haujlwm Saib Xyuas Kev Txhim Kho Kev Lag Luam (KIAT) thiab Ministry of Knowledge Economy (MKE), thiab ib feem los ntawm Cov Khoom Noj Khoom Noj Khoom Haus Zoo Tshaj Plaws , Ministry of Agriculture, Food and Rural Affairs (MAFRA; grant number 113034-3).
Kev tsis sib haum xeeb ntawm kev txaus siab Cov neeg sau ntawv tshaj tawm tias tsis muaj kev tsis sib haum xeeb txog nyiaj txiag los tshaj tawm cov txiaj ntsig no.
Cov ntaub ntawv
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