Cov txiaj ntsig ntawm Cistanche Deserticola Bushen Huoxue Daim Ntawv Pom Zoo Ntawm Oxidative Stress, PI3K/Akt, Bcl-2/Bax nyob rau hauv nas nrog rau mob raum tsis ua haujlwm
Mar 28, 2023
LIN Guan-Hao, Huang Mei, WU Mei
(Dept. ntawm Pharmacy, Haikou Tsev Kho Mob ntawm Tsob Ntoo Suav Tshuaj, Haikou 570216 Hainan, Suav)
Abstract: Lub Hom Phiaj Los soj ntsuam cov txiaj ntsig kho mob thiab cov txheej txheem ntawm covLub raum Tonifying thiab ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Tshuaj(BHP, tshuaj ntsuab feem ntau nrog rau kev ua kom lub raum ntxiv thiab ua kom cov ntshav) hauv cov nas uas muaj lub raum tsis ua haujlwm. Txoj Kev Plaub caug SD nas tau muab faib ua ib pab pawg (10 nas), ib pab pawg qauv (9 nas), BHP pawg qis (10 nas), thiab pab pawg neeg siab ( 10 nas). Tsuas yog cov pab pawg ib txwm muaj, cov nas hauv txhua pab pawg tau rov ua dua nrog cov qauv rau lub raum tsis ua haujlwm los ntawm adenine gavage. Tom qab ua tiav kev rov ua tiav, cov nas nyob hauv BHP cov pab pawg qis thiab cov koob tshuaj siab tau muab 0.52 thiab 2.08 g / kg ntawm BHP los ntawm gavage, feem, ib hnub ib zaug rau 28 hnub sib law liag. . Tom qab qhov kawg ntawm kev tswj hwm, cov qib ntshav hauv lub raum qhia txog ntshav urea nitrogen (BUN), ntshav creatinine (SCr) thiab uric acid (UA), thiab qib ntawm oxidative stress indexes malondialdehyde (MDA) thiab superoxide dismutase (SOD) hauv lub raum. Cov ntaub so ntswg tau ntsuas los ntawm enzyme-linked immunosorbent assay (ELISA), cov kev hloov pauv histopathological ntawm nas raum cov ntaub so ntswg tau pom los ntawm hematoxylin-eosin (HE) staining, thiab cov protein qhia ntawm phosphatidylinositol{15}}kinase (PI3K), protein kinase B. (Akt), B cell lymphoma 2 (Bcl-2) thiab Bcl{19}} txuam nrog X protein (Bax) hauv lub raum cov ntaub so ntswg tau kuaj pom los ntawm Western Blot. Cov txiaj ntsig (1) Muab piv nrog cov pab pawg ib txwm muaj, theem ntawm BUN, SCr thiab UA tau nce ntxiv hauv pawg qauv (P＜0.05); piv nrog rau pawg qauv, qib ntawm BUN, SCr thiab UA tau poob qis hauv BHP cov pab pawg tsawg thiab koob tshuaj siab (P＜0.05). tau txo qis hauv pawg qauv (P＜0.05); piv nrog rau pawg qauv, MAD qib tau txo qis thiab SOD qib tau nce hauv BHP cov pab pawg qis thiab qib siab (P＜0.05); (3) Piv nrog rau pawg ib txwm , pathological hloov xws li me me necrosis thiab inflammatory cell infiltration, tubular dilatation thiab glomerular deformation tshwm sim nyob rau hauv lub raum cov ntaub so ntswg ntawm cov qauv pab pawg neeg; piv nrog rau cov qauv pab pawg neeg, cov pathological tej yam kev mob nyob rau hauv ob qho tib si BHP qis- thiab high-dose pawg tau txhim kho. (4) Piv nrog rau cov pab pawg ib txwm, cov protein qhia theem ntawm PI3K thiab Akt nyob rau hauv lub raum cov ntaub so ntswg nyob rau hauv cov qauv pab pawg neeg yog ho nce (P＜ 0.05); Cov ntaub so ntswg hauv lub raum hauv BHP cov pab pawg tsawg thiab cov koob tshuaj siab tau txo qis (P＜0.05). txo qis thiab cov protein qhia theem ntawm Bax tau nce (P＜0.05); piv nrog rau cov qauv pab pawg, qhov qhia theem ntawm Bcl -2 protein nyob rau hauv lub raum cov ntaub so ntswg hauv BHP cov pab pawg tsawg- thiab cov koob tshuaj tau nce ntxiv thiab Cov protein qhia theem ntawm Bax tau txo qis (P＜0.05). Xaus BHP muaj kev cuam tshuam hauv kev txhim kho lub raum raug mob hauv cov nas uas muaj lub raum tsis ua haujlwm ntev los ntawm cov txheej txheem cuam tshuam nrog kev cuam tshuam ntawm PI3K / Akt signaling txoj hauv kev thiab kev tswj hwm ntawm kev qhia txog apoptotic protein.
Ntsiab lus: Lub raum Tonifying thiab Ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Ntawv Tshuaj; mob raum tsis ua haujlwm; oxidative stress; phosphatidylinositol-3-kinase (PI3K); protein kinase B (Akt); B cell lymphoma 2 (Bcl-2); Bcl-2 txuam X protein (Bax); nas
Lub raum tsis ua haujlwm (CRF) yog ib hom kev kho mob uas ua rau lub raum puas vim yog cov tshuaj tiv thaiv kab mob autoimmune, heredity, noj zaub mov, kis kab mob, thiab lwm yam, ua rau lub raum tsis muaj zog, lossis ua tiav kev ua haujlwm. Qhov xwm txheej tau nce zuj zus txhua xyoo, thiab nws nyuaj rau kho [1-3]. Nyob rau hauv xyoo tas los no, cov kev kho mob ntawm cov tshuaj suav tshuaj ntawm lub raum tsis ua haujlwm tau nce zuj zus tuaj. Lub raum tsis ua haujlwm ntev yog nyob rau hauv qeb ntawm "Guange" thiab "edema" hauv cov tshuaj suav tshuaj. Tus kab mob no feem ntau tshwm sim nyob rau hauv lub raum, uas yog ib tug syndrome ntawm deficiency thiab tshaj. Lub ntsiab pathogenesis yog lub raum tsis muaj peev xwm thiab ntshav stasis, thiab kev kho mob tseem ceeb yog ua kom lub raum ntxiv thiab txhawb cov ntshav ncig [4]. Cov kev tshawb fawb yav dhau los tau pom tias txoj kev ua kom lub raum ua kom lub raum thiab ua kom cov ntshav khiav tau zoo tuaj yeem txhim kho cov mob ntawm cov neeg mob lub raum tsis ua haujlwm, txhim kho cov ntshav microcirculation, thiab inhibit inflammatory reactions hauv lub cev [5-6]. Txawm li cas los xij, cov txheej txheem kho mob ntawm txoj kev tonifying lub raum thiab ua kom cov ntshav ncig rau lub raum tsis ua haujlwm ntev tsis meej meej. Yog li, qhov kev tshawb fawb no tau tsim tsa tus qauv nas ntawm lub raum tsis ua haujlwm ntxiv los soj ntsuam qhov cuam tshuam thiab cov txheej txheem ntawm tus kheej-formulated mis ntawm tonifying lub raum thiab ua kom cov ntshav ncig, npaj siab los muab cov ntaub ntawv siv rau kev kho mob daim ntawv thov ntawm txoj kev tonifying lub. raum thiab activating ntshav ncig rau kev kho mob raum tsis ua haujlwm. Cov txiaj ntsig tshawb fawb tau tshaj tawm raws li hauv qab no.
Suav tshuaj cov ntaub ntawv xws liCistanche deserticola
Nyem qhov no mus saib Cistanche rau raum kab mob khoom
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1 Cov ntaub ntawv thiab cov txheej txheem
1.1 Kev sim tsiaj
40 SPF-theem noj qab haus huv 6- cov txiv neej hnub nyoog ib lim piam SD nas, nrog lub cev hnyav ntawm 130 ~ 170 g, tau yuav los ntawm Beijing Innokai Technology Co., Ltd. nrog rau daim ntawv tso cai tsim tsiaj ntawm SCXK (Beijing) { {6}}. Ua ntej qhov kev sim, cov nas tsuag tau yoog raws li 2 lub lis piam, pub dawb nrog dej, nrog rau qhov kub thiab txias ntawm (24 ± 1) degree, txheeb ze av ntawm 40% mus rau 60 feem pua, thiab lub voj voog ntawm 12 teev lub teeb thiab 12 teev. tsaus ntuj. Txoj kev tshawb fawb no tau pom zoo los ntawm Pawg Saib Xyuas Kev Ncaj Ncees ntawm Haikou Traditional Chinese Medicine Hospital.
1.2 Cov tshuaj, cov tshuaj reagents, thiab cov cuab yeej siv
Cov tshuaj rau tonifying lub raum thiab activating ntshav ncigmuaj 10 g ntawm siav rehmannia, 10 grams ntawmcistanche deserticola,10 g ntawm Xianling spleen, 15 g ntawm raw astragalus, 5 g ntawm wine rhubarb, 10 g ntawm salvia miltiorrhiza, 15 g ntawm angelica. Txhua yam khoom siv tshuaj suav tshuaj yog yuav los ntawm Haikou Traditional Chinese Medicine Hospital. Pa decoction, pom, concentration rau 4 g / mL ntawm crude tshuaj, khaws cia rau standby. Adenine (Shanghai Jizhi Biochemical Technology Co., Ltd., batch naj npawb: A800684); Cov khoom siv kuaj ntshav creatinine (SCr) (Beijing Lingbao Technology Co., Ltd.); Urea nitrogen (BUN) thiab uric acid (UA) kuaj cov khoom (Beijing Boaze Technology Co., Ltd.); Malondialdehyde (MDA) thiab superoxide dismutase (SOD) kuaj cov khoom (Shanghai Qizheng Biotechnology Co., Ltd.); Luav anti intracellular phosphatidylinositol 3 kinases (PI3K) polyclonal antibody, luav anti-protein kinase B (Akt) polyclonal antibody (Shanghai Jinsui Biotechnology Co., Ltd.); Luav anti-B lymphocyte qog 2 noob (Bcl-2) antibody, luav anti-Bcl-2 ntsig txog X protein (Bax) antibody (Guangzhou Chuanrong Biotechnology Co., Ltd.); Tshis los tiv thaiv luav IgG theem nrab antibody (Shanghai Biyuntian Biotechnology Co., Ltd.). MPR A100 enzyme marker [Yasuwang (Shanghai) Trading Co., Ltd.]; Gel imager (Bio-Rad Company, USA); ADVIA2400 siab tsis siv neeg biochemical analyzer (Siemens tuam txhab).
Suab puam ginseng
1.3 Kev ua qauv, pab pawg, thiab kev tswj hwm
Xaiv 10 nas raws li pawg ib txwm, thiab 30 tus nas ntxiv los tsim tus qauv raum tsis ua haujlwm. Txiav txim siab seb tus qauv puas ua tiav raws li qib ntshav ntawm lub raum kev ua haujlwm ntawm lub raum ntsuas thiab lub raum pathological hloov pauv hauv cov qauv nas [7]. Txoj kev ua qauv [8]: 250 mg / kg adenine tau muab los ntawm gavage ib hnub rau 28 hnub sib law liag. Ib tug nas tuag thaum lub sij hawm ua qauv. Cov nas ua qauv zoo tau muab faib ua pawg qauv (9 nas), pawg qis ntawm Bushen Huoxue Formula (10 nas), thiab pawg siab ntawm Bushen Huoxue Formula (10 nas). Tom qab ua tiav kev ua qauv, cov koob tshuaj niaj hnub ntawm cov neeg laus tau hloov mus rau hauv koob tshuaj nas raws li "Pharmacological Experimental Methods", thiab tau teeb tsa raws li koob tshuaj raum Tonifying thiab Ntshav Activating Formula. Cov pab pawg tsawg thiab siab ntawm Bushen Huoxue Formula tau muab 0.52 thiab 2.08 g / kg ntawm Bushen Huoxue Formula daws los ntawm gavage. Cov pab pawg ib txwm muaj thiab cov qauv pab pawg tau muab qhov sib npaug ntawm lub cev saline los ntawm gavage. Ib hnub rau 28 hnub sib law liag.
1.4 Kev soj ntsuam ntsuas thiab cov txheej txheem
1. 4. 1. Kev kuaj xyuas lub raum ua haujlwm ntsuas
Tom qab qhov kev cuam tshuam, cov ntshav tau sau los ntawm lub plab aorta ntawm cov nas, thiab cov ntshav kuaj tau raug cais tawm tom qab centrifugation. Enzyme-linked immunosorbent assay tau siv los txheeb xyuas cov theem ntawm SCr, BNU, thiab UA hauv nas hauv txhua pab pawg. Kev ua haujlwm tau ua tiav raws li cov lus qhia ntawm cov khoom siv.
1. 4. 2 HE staining
Cov ntaub so ntswg ntawm lub raum tau muab tshem tawm, tsau, lub cev qhuav dej, paraffin-embedded, sectioned, stained, dehydrated, thiab kaw dua. Pathological hloov nyob rau hauv nas lub raum ntaub so ntswg tau pom thiab yees duab.
1. 4. 3 Kev soj ntsuam ntawm oxidative stress indicators nyob rau hauv lub raum ntaub so ntswg
Noj cov nqaij nas raum, npaj homogenate, centrifuge, thiab cais cov supernatant. Raws li cov lus qhia reagent, siv cov enzyme-linked immunosorbent assay txhawm rau txheeb xyuas cov qib MDA thiab SOD.
1. 4. 4 Protein Immunoblotting Test
Tom qab noj homogenate los ntawm nas lub raum cov ntaub so ntswg, ntxiv RIPA kom rho tawm tag nrho cov protein, centrifuge ntawm 3000 r / min (centrifugation radius: 15 cm) rau 10 feeb, coj lub supernatant thiab ntsuas cov ntsiab lus protein. Tom qab cov protein sib cais los ntawm gel electrophoresis, membrane hloov, sealing, thiab TBST tu, thawj cov tshuaj tiv thaiv PI3K, Akt, Bcl-2, Bax, thiab - Actin (diluted los ntawm volume ratio ntawm 1: 1000) yog incubated hmo ntuj ntawm 4. degree, zaj duab xis tau ntxuav, thiab ib tug thib ob antibody (diluted los ntawm ntim piv ntawm 1:5000) ntxiv mus rau incubate nyob rau hauv chav tsev kub rau 2 teev. ECL tau nthuav tawm rau kev txhim kho, thiab tus nqi grey ntawm electrophoretic bands tau txheeb xyuas siv ImageJ software. Cov txiaj ntsig Raws li lub hom phiaj ntawm cov protein thiab cov khoom siv sab hauv (- Kev Ua) greyscale tus nqi piv txwv.
1.5 Cov txheej txheem txheeb cais
SPSS 25.0 statistical software tau siv los txheeb xyuas cov ntaub ntawv. Cov ntaub ntawv ntsuas tau qhia tias txhais tau tias ± tus qauv sib txawv (x ± s). Univariate tsom xam ntawm qhov sib txawv tau ua rau kev sib piv ntawm ntau pab pawg, thiab t-test tau siv rau kev sib piv ntawm ob pawg. Qhov sib txawv yog qhov tseem ceeb thaum P<0.05 was used.
2 Kev tshwm sim
2.1 Kev sib piv ntawm lub raum ua haujlwm Performance index theem ntawm nas nyob rau hauv txhua pab pawg
Table 1 qhia tau hais tias piv nrog cov pab pawg ib txwm, theem ntawm BUN, SCR, thiab UA hauv pawg qauv tau nce (P<0.05); Compared with the model group, the levels of BUN, SCr, and UA in the low and high dose groups of Bushen Huoxue Formula decreased (P<0.05) in a dose-dependent manner. Comparison of renal histopathological changes of rats in each group Figure 1 shows that the renal tissue structure of rats in the normal group is complete, with an orderly arrangement of glomeruli and renal tubules, without significant abnormal changes; Compared with the normal group, the model group had mild necrosis and inflammatory cell infiltration in the renal tissue, renal tubular dilation, glomerular deformation, and other pathological changes; Compared with the model group, the pathological conditions of the low and high dose groups of Bushen Huoxue Recipe were improved.
Table 1 Kev sib piv ntawm qib ntawm lub raum kev ua haujlwm ntsuas ntawm ntau pawg nas（x ± s）
Nco tseg: ① P ＜ 0.05, piv nrog cov pab pawg ib txwm muaj; ② Ib<0.05, compared with the model group; ③ P<0.05, compared with the low dose group of Bushen Huoxue Formula
Daim duab 1 Kev sib piv ntawm cov kev hloov histopathological ntawm nas raum cov ntaub so ntswg ntawm ntau pawg（Nws staining,×400）
Kev sib piv ntawm oxidative stress index theem hauv lub raum cov ntaub so ntswg ntawm nas nyob rau hauv txhua pab pawg neeg
Table 2 qhia tau hais tias piv nrog cov pab pawg ib txwm, MDA qib hauv lub raum cov ntaub so ntswg ntawm cov qauv pab pawg nas tau nce, thaum SOD qib qis (P<0.05); Compared with the model group, the MDA level in the kidney tissue of rats in the low and high dose groups of Bushen Huoxue Recipe decreased, while the SOD level increased (P<0.05).
Kev qhia theem ntawm PI3K thiab Akt proteins nyob rau hauv lub raum cov ntaub so ntswg ntawm nas nyob rau hauv txhua pab pawg neeg
Muab piv rau Table 3 thiab daim duab 2, cov txiaj ntsig tau pom tias piv nrog cov pab pawg ib txwm muaj, cov lus qhia theem ntawm PI3K thiab Akt cov proteins hauv lub raum cov ntaub so ntswg ntawm cov qauv pab pawg tau nce ntau (P<0.05); Compared with the model group, the expression levels of PI3K and Akt proteins in the kidney tissue of rats in the low and high dose groups of Bushen Huoxue Formula decreased (P<0.05).
Table 2 Kev sib piv ntawm cov qib ntawm oxidative stress indicators nyob rau hauv lub raum cov ntaub so ntswg ntawm ntau pawg nas （x ± s）
Nco tseg: ① P ＜ 0.05, piv nrog cov pab pawg ib txwm muaj; ② Ib<0.05, compared with the model group; ③ P<0.05, compared with the low dose group of Bushen Huoxue Formula
Table 3 Kev sib piv ntawm cov protein qhia qib ntawm PI3K thiab Akt hauv lub raum cov ntaub so ntswg ntawm txhua pawg nas（x ± s）
Nco tseg: ① P ＜ 0.05, piv nrog cov pab pawg ib txwm muaj; ② Ib<0.05, compared with the model group; ③ P<0.05, compared with the low dose group of Bushen Huoxue Formula
Kev nthuav qhia theem ntawm Bcl-2 thiab Bax proteins nyob rau hauv lub raum cov ntaub so ntswg ntawm nas nyob rau hauv txhua pab pawg neeg
Sib piv cov txiaj ntsig hauv Table 4 thiab Daim duab 3, nws tau pom tias piv nrog cov pab pawg ib txwm muaj, qhov qhia theem ntawm Bcl -2 cov protein nyob rau hauv lub raum cov ntaub so ntswg ntawm nas nyob rau hauv cov qauv pab pawg neeg poob qis, thaum qhia theem ntawm Bax. protein ntau nce ntxiv (ob leeg P<0.05); Compared with the model group, the expression level of Bcl-2 in the kidney tissue of rats in the low and high dose groups of Bushen Huoxue Formula increased, while the expression level of Bax decreased (both P<0.05).
Daim duab 2 Western Blot electropherograms ntawm PI3K thiab Akt proteins hauv lub raum cov ntaub so ntswg hauv txhua pawg nas
Table 4 Kev sib piv ntawm cov protein qhia theem ntawm Bcl-2 thiab Bax hauv lub raum cov ntaub so ntswg ntawm ntau pawg nas （x ± s）
Nco tseg: ① P ＜ 0.05, piv nrog cov pab pawg ib txwm muaj; ② Ib<0.05, compared with the model group; ③ P<0.05, compared with the low dose group of Bushen Huoxue Formula
Daim duab 3 Western Blot electropherograms ntawm Bcl-2 thiab Bax proteins hauv lub raum cov ntaub so ntswg hauv txhua pab pawg nas
3 Kev sib tham
Adenine induced chronic renal failure model yog ib txoj hauv kev los tshuaj xyuas cov tshuaj kho mob rau lub raum tsis ua haujlwm thiab qhia meej txog cov txheej txheem ntawm kev siv tshuaj [9]. Cov leeg nqaij metabolite SCr, purine metabolite kawg khoom UA, thiab plasma nitrogen-muaj cov khoom xyaw BUN khaws cia hauv cov ntshav thaum lub raum ua haujlwm puas tsuaj thiab yog cov cim tseem ceeb rau kev kuaj lub raum ua haujlwm [10-11]. Hauv txoj kev tshawb no, tus qauv rau lub raum tsis ua haujlwm tau tsim los ntawm adenine-induced lub raum puas hauv nas. Cov txiaj ntsig ntawm kev ua qauv qhia tau hais tias hauv pawg qauv, qib ntawm SCr, BUN, thiab UA hauv cov ntshav nce ntxiv, inflammatory cell infiltration tshwm sim nyob rau hauv lub raum cov ntaub so ntswg, lub raum tubular dilation, glomerular deformation, thiab lwm yam pathological hloov, qhia tau hais tias tus qauv yog. ua tiav. Tom qab kev kho mob nrog ntau qhov sib txawv ntawm Bushen Huoxue Formula, qib ntawm SCr, BUN, thiab UA hauv cov ntshav ntawm nas txo qis, thiab lub raum cov ntaub so ntswg thiab kev ua haujlwm ntawm lub cev tau txhim kho mus rau qhov sib txawv, qhia tias.Lub raum Tonifying thiab ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Tshuajmuaj kev tiv thaiv rau lub raum ntawm cov nas uas muaj lub raum tsis ua haujlwm ntev.
Suav cov khoom siv tshuaj xws li cistanche deserticola
Qee cov kev tshawb fawb tau pom tias oxidative kev nyuaj siab tuaj yeem txhawb txoj kev loj hlob ntawm lub raum tsis ua haujlwm, thiab qib ntawm oxidative kev nyuaj siab nyob rau hauv lub raum cov ntaub so ntswg nce ntxiv nrog rau cov qib ntawm lub raum kev puas tsuaj. SOD yog ib chav kawm ntawm cov yam ntxwv uas tuaj yeem tiv thaiv cov kab mob inflammatory, thiab MDA yog cov khoom decomposition ntawm lipid peroxidation hauv lub cev [13]. Yog li, txhim kho SOD qib, txo MDA qib, thiab txo qis oxidative kev ntxhov siab nyob rau hauv lub raum cov ntaub so ntswg tuaj yeem cuam tshuam kev loj hlob ntawm lub raum tsis ua haujlwm. Cov txiaj ntsig ntawm txoj kev tshawb fawb no kuj tau lees paub tias tom qab kev cuam tshuam ntawm Bushen Huoxue Formula, qib ntawm oxidative stress indicators hauv nas poob, qhia tias.Lub raum Tonifying thiab ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Tshuajtuaj yeem ua lub luag haujlwm hauv antioxidant kev ntxhov siab thiab kev tiv thaiv kev tiv thaiv, thiab inhibit kev loj hlob ntawm lub raum tsis ua haujlwm.
Cistanche tshuaj yej
PI3K yog ib qho tseem ceeb ntawm cov teeb liab transduction txoj hauv kev ntawm kev loj hlob yam teeb liab transduction. Akt yog ib qho serine protein kinase uas yog nyob rau hauv qhov qis qis ntawm PI3K. Nyob rau hauv tus txheej txheem ntawm lub raum tsis ua hauj lwm, txoj kev PI3k / Akt yog koom nrog inflammatory cov tshuaj tiv thaiv, parenchymal cell apoptosis, sib txawv, thiab collagen tsim thiab deposition. Lie Beifeng et al. [14] pom tias qhov kev qhia ntawm phosphorylated Akt nyob rau hauv lub raum tubulointerstitium ntawm ntshav qab zib nephropathy qauv nas nce ntxiv, qhia tias PI3K / Akt signaling pathway yuav koom nrog hauv cov txheej txheem ntawm lub raum tubular lesions. Nyob rau hauv lub raum tsis ua hauj lwm qauv nas, PTEN / PI3k / Akt signaling pathway yog koom nrog hauv cov txheej txheem ntawm lub raum interstitial fibrosis [15]. Lwm cov kev tshawb fawb tau pom tias 5/6 nephrectomized nas yuav inhibit TGF los ntawm / PI3K / Akt signaling pathway, yog li txo SCr, txo lub raum fibrosis, thiab txhim kho lub raum ua haujlwm hauv nas [16]. Runyan et al. [17] pom tias TGF hauv glomerular cells- Stimulating Akt phosphorylation thiab tswj PI3k inhibitors rau glomerular cells tuaj yeem txo TGF- qhia. Cov txiaj ntsig ntawm txoj kev tshawb no qhia tau hais tias Bushen Huoxue Formula tuaj yeem cuam tshuam qhov kev qhia ntawm PI3k / Akt protein nyob rau hauv lub raum cov ntaub so ntswg ntawm cov nas uas muaj lub raum tsis ua haujlwm, inhibit PI3k / Akt signaling pathway, thiab yog li ncua kev loj hlob ntawm tus kab mob hauv nas nrog rau lub raum ntev. ua tsis tiav. Rau lub raum cov ntaub so ntswg, apoptosis muaj ob lub txiaj ntsig [18]: Ntawm ib sab, apoptosis tuaj yeem tshem tawm cov kab mob inflammatory invasion thiab cov hlwb txawv txav, txhawb kev kho ntawm lub raum cov ntaub so ntswg thiab rov ua haujlwm ntawm lub raum; Ntawm qhov tod tes, apoptosis feem ntau ua rau txo qis ntawm lub raum hlwb, uas ua lub luag haujlwm tseem ceeb hauv qhov tshwm sim ntawm glomerulosclerosis thiab tubular atrophy, thiab tuaj yeem ua rau mob raum mob hnyav dua. Kev tshawb fawb [19] qhia tau hais tias tus naj npawb ntawm apoptosis nyob rau hauv lub raum tubular hlwb nyob rau hauv cov neeg mob lub raum kab mob yog ho ntau dua li cov neeg noj qab nyob zoo, thiab tus naj npawb ntawm apoptosis nyob rau hauv lub raum ntaub so ntswg yog ze ze rau lub raum interstitial raug mob Performance index, raum fibrosis index, tso zis. qib protein, thiab qib ntawm deterioration ntawm lub raum ua haujlwm. Bcl -2 tsev neeg apoptotic protein tuaj yeem muab faib ua peb pawg: proapoptotic txhawb nqa proteins, proapoptotic effector proteins, thiab anti-apoptotic proteins. Lub antagonism ntawm proapoptotic proteins thiab anti-apoptotic proteins txiav txim lub apoptosis txheej txheem ntawm cov loj loj cell [20]. Kev tshawb fawb [18] qhia tau hais tias inducing upregulation ntawm Bcl -2 qhia yuav txo tau cell apoptosis. Hauv cov qauv mob raum raug mob thiab cov qauv ua rau lub raum tsis ua haujlwm, tshem tawm Bax thiab Bak noob nyob rau hauv proximal lub raum tubular epithelial hlwb tuaj yeem txo cov apoptosis ntawm lub raum tubular epithelial hlwb thiab txo cov qib ntawm lub raum tsis ua haujlwm [18]. Cov txiaj ntsig ntawm txoj kev tshawb no qhia tau hais tias qhov kev qhia ntawm Bcl -2 protein nce thiab kev qhia ntawm Bax protein txo qis hauv cov kab mob raum tsis ua haujlwm nas kho nrogLub raum Tonifying thiab ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Tshuaj. Qhov no tej zaum yuav yog vim Bushen Huoxue Recipe inhibits qhov kev qhia ntawm apoptosis-txog cov proteins Bcl-2 thiab Bax downstream ntawm PI3K/Akt signaling pathway, inhibits lub raum cell apoptosis, li no alleviating raum fibrosis, thiab ncua lub deterioration ntawm lub raum ua hauj lwm.
Cistanche deserticola kuv
Hauv cov ntsiab lus,Lub raum Tonifying thiab ntshav Activating Formula muajCistanche deserticolathiab Lwm Cov Tshuajtuaj yeem txo qhov kev puas tsuaj rau lub raum hauv cov nas uas muaj lub raum tsis ua haujlwm, thiab nws cov txheej txheem yuav cuam tshuam rau inhibiting PI3K / Akt signaling pathway thiab tswj kev qhia ntawm apoptotic proteins Bcl-2 thiab Bax. Tsis tas li ntawd, lub raum fibrosis thiab deterioration ntawm lub raum kev ua haujlwm ntawm nas nrog adenine intragastric tswj yuav muaj feem cuam tshuam rau lub raum tubular raug mob, thiab cov txheej txheem sib sib zog nqus yuav tsum tau kawm ntxiv.
siv:
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