Kev txiav txim siab ntawm Tag Nrho Saponins Cov ntsiab lus hauv Boschniakia Rossica Thiab Herba Cistanches Los ntawm Spectrophotometry

2. 9. Kev tshuaj xyuas cov txheej txheem rho tawm ntawm cov khoom siv tshuaj.

Tom qab sau cov ntaub ntawv thiab tshuaj xyuas ntau cov ntaub ntawv thiab cov ntawv xov xwm, 3 yam xws li cov khoom siv-rau-kua piv (A), hnyav concentration (B), thiab lub sij hawm rho tawm (C) raug xaiv los ua qhov tseem ceeb rau kev soj ntsuam rau ob qho tib si Grasshopper thiab Cistanche deserticola. [20], txhua qhov teeb meem tau teeb tsa nrog 3 qib, qhov ntsuas ntsuas tautag nrho glycosidehuab hwm coj %, thiab ib qho kev sim orthogonal tau tsim. Cov txiaj ntsig tau pom nyob rau hauv Table 7 thiab Table 8.

Nws yuav siv sij hawm ntev npaum li cas rau CISTANCHE ua haujlwm?

Cov txiaj ntsig ntawm kev txheeb xyuas ntau yam qhia tau hais tias qhov kev txiav txim ntawm qhov cuam tshuam ntawm Grasshopper yog: hnyav concentration> rho tawm lub sij hawm> khoom-kua piv. Yog li ntawd, qhov zoo tshaj extraction tej yam kev mob ntawmCistanche deserticola tag nrho glycosidestau txiav txim siab raws li A2B3C1, uas yog, qhov zoo tshaj plaws extraction txoj kev npaj yog rho tawm ib zaug nrog 90% ethanol, thiab cov khoom-kua piv yog 1:20; kev txiav txim ntawm influencing yam ntawmCistanche deserticolayog: solid-liquid ratio > naj npawb ntawm extractions > Solvent concentration.

Yog li ntawd, qhov zoo tshaj plaws extraction tej yam kev mob A2B3C2 tau txiav txim siab, yog li qhov zoo tshaj plaws extraction txoj kev npaj yog ib tug solid-kua piv ntawm 1:20 thiab extraction ob zaug nrog 90% ethanol tov.

2. 10 Kev xaiv wavelength rau kev txiav txim siab ntawm tag nrho glycosides

Tom qab kuaj xyuas qhov nqus dej ntawm cov tshuaj tswj thiab cov tshuaj ntsuam xyuas ntawm 400 txog 800nm, cov txiaj ntsig tau pom tias muaj qhov sib txawv me ntsis ntawm tus nqi nqus ntawm salidroside cov khoom siv ntawm 520nm thiab 580nm, thaum tom qab xim kev loj hlob ntawm Cachinensis thiabCistanche deserticola, Lub wavelength scanning tshwm sim qhia tau hais tias ob leeg muaj qhov siab tshaj plaws nqus nyob ze 523nm. Tom qab tshawb xyuas ntau cov ntaub ntawv, xav txog cov xwm txheej tiag tiag thiab cov khoom siv tshuaj lom neeg ntawm Grasshopper thiab Cistanches, thaum kawg peb xaiv salidroside li Grasshopper. thiab cov ntaub ntawv siv rau kev txiav txim siab ntawm tag nrho glycosides ntawm Cistanche deserticola, thiab kev tshawb pom wavelength tom qab xim kev loj hlob tau txiav txim siab yog 523nm.

2. 11 Kev tshawb fawb txog kev rho tawm cov khoom siv tshuaj thiab cov kuab tshuaj extraction

Nyob rau theem pib ntawm qhov kev sim, ntau txoj kev rho tawm xws li cua sov reflux, Soxhlet extraction, ultrasound, thiab txias-soaking tau muab piv. Cov txiaj ntsig tau pom tias tag nrho cov glycosides tau txais los ntawm cov txheej txheem cua sov reflux extraction yog siab dua li ntawm lwm txoj kev, yog li cov txheej txheem cua sov reflux tau xaiv los rho cov nyom. Cistanche deserticola thiab Cistanche deserticola tshuaj ntsuab.

Xav txog qhov polarity thiab solubility ntawm phenylpropanoid saponins, peb cov kuab tshuaj extraction ntawm methanol, ethanol, thiab n-butanol tau tshawb xyuas thiab tshuaj xyuas. Cov txiaj ntsig tau pom tias cov nyhuv extraction ntawm ethanol zoo dua, yog li ethanol ntawm ntau qhov sib txawv tau siv los ua cov kuab tshuaj extraction hauv qhov kev sim no.

3 Kev sib tham

Hauv qhov kev sim no, salidroside tau siv los ua cov khoom siv thawj zaug los txiav txim siab tag nrho cov ntsiab lus glycoside hauv Grasshopper thiab Cistanche deserticola. Tag nrho cov ntsiab lus glycoside hauv Grasshopper qauv yog 10. 61%, tag nrho cov ntsiab lus glycoside hauvCistanche deserticolapiv txwv yog 10. 07%. Txawm hais tias spectrophotometry yog ib txoj kev txiav txim siab txog cov ntsiab lus classic thiab paub tab, xim cov tshuaj tiv thaiv ntawm tag nrho glycosides thiab vanillin-glacial acetic acid thiab perchloric acid kuj tseem rhiab heev, tab sis tus ntes yuav tsum tau ua kom raug thiab ceev cov txheej txheem los xyuas kom meej cov txiaj ntsig zoo hauv kev sim. .

Nyob rau hauv cov ntsiab lus, txoj kev no muaj siab precision, simplicity thiab zoo stability, thiab yuav siv tau rau kev txiav txim ntawm tag nrho cov glycosides cov ntsiab lus nyob rau hauv Grasshopper thiab Cistanche deserticola. Cov tshuaj muaj pes tsawg leeg thiabCov tshuaj pharmacological ntawm Cistanche deserticolathiab Cao Congrong zoo ib yam, uas muab lub hauv paus theoretical rau kev siv keeb kwm ntxiv ntawmCistanche deserticolathiab Cao Congrong, tawm tswv yim tias kev txhim kho thiab kev siv Cao Congrong muaj kev thov dav dav. Cov txheej txheem rho tawm thiab kev ua haujlwm lom neeg ntawm obtag nrho glycosidestab tom kawm ntxiv.

Cov ntaub ntawv:

[1] Yu Ying, Zhao Shuchun, Xiu Jingchang. Cov nroj tsuag tsis tshua muaj nyob hauv roob Changbai: Amaranth officinale ［J］． Tuam Tshoj Wild Plant Resources, 1996, (3): 22.

[2] Chen Qinghong, Zhang Rui, Feng Xiuchun, thiab lwm yam. Tam sim no cov xwm txheej ntawm kev tshawb fawb thiab kev tswj hwm kev kub ntxhov ntawm Amaranth officinale hauv Changbai Roob ［J］. Niaj hnub no Agricultural Science thiab Technology, 2015, (14): 80；.

[3] Li Caifeng, Wang Xiaoqin, Liu Yong, thiab lwm yam. Kev tshawb fawb nce qib ntawm cov tshuaj muaj pes tsawg leeg thiab cov tshuaj pharmacological ntawm Amaranth officinale［J］. Suav Herbal Tshuaj, 2014, 45(7): 1016.

[4] Sun Cang, Chang Guiying, Jiao Yanchun, thiab lwm yam. Sib piv ntawm Grasshopper thiab Cistanche deserticola［J］. Phau ntawv Journal ntawm Jilin Agricultural University, 1996, 18 (ntxiv ntxiv): 118.

[5] Jin Meishan, Li Lianhua, Jiang Fengchun, thiab lwm yam. Kawm txog kev tiv thaiv kev laus ntawm Escherichia coli ntawm hepatocytes ntawm suckling nas cultured hauv vitro［J］］］］］ Phau ntawv Journal of Medicine of Yanbian University, 1997, 20(4): 223.

[6] Chen Hua, Dong Lis. Kev tshawb fawb nce qib ntawm kev tiv thaiv kev laus ntawm Cistanche deserticola polysaccharide ］J］. Suav Journal of Coal Industry Medicine, 2012, 15(2): 310.

[7] Yu Qinghai, Pu Guangchun, Niu Yufen, thiab lwm yam. Suav Materia Medica, 1993, 16 (7): 32.

[8] Zhou Haitao, Cao Jianmin, Lin Qiang, et al. Cov teebmeem ntawm Cistanche deserticola ntawm kev ua luam dej tas li thiab muaj peev xwm antioxidant ntawm myocardial mitochondria hauv cov nas ［J］. Suav Journal of Experimental Prescriptions, 2012, 18(6):

229.

[9] Park Yuren, Jiang Yushun, Li Yingxin. Kev cuam tshuam ntawm Escherichia coli ntawm kev tiv thaiv kab mob ntawm Kupffer hlwb hauv lub siab raug mob ［J］ Suav tshuaj ntsuab, 1994, 25(4): 200.