Kev kawm sib piv ntawm cov microbial txwv kuaj thiab cov txheej txheem ntawm TCM Decoction Phaj nrog United States Tshuaj, Europeanese pharmacopeia

2 Tswj cov kab mob kuaj xyuas

Tswj kab mob tshuaj xyuas yog ib yam khoom tseem ceeb hauv kev kuaj xyuasTshuaj Hmoob Suav TshuajCov. Cov kev tswj hwm tus kab mob plaub yam uas muaj cov kab mob bile-resistant Qhov kev sib piv ntawm lawv txoj kev kuaj xyuas tau qhia hauv Table-are Ib qho ntxiv, txoj kev soj ntsuam ntawm Staphylococcus aureus tau ntxiv rau USP43 thiab JP17; USP43 kuj tau txhawb txoj kev kuaj tshuaj ntawm cov txee hauv kev ntawm kev tiv thaiv thiab yog thawj zaug los tshaj tawm cov tswvyim ntawm kev ntsuas cov kev pheej hmoo tsis tsim nyog. Ib qho ntxiv, USP43 tsuas yog tau ua rau txoj cai ntawm kev coj noj coj ua hauv cov tshuaj lom neeg Suav, thiab tsis tau hais txog txoj kev coj ua ntawm kev ua tiav cov qauv ntsuas; Thaum Ep10.0, JP17 thiab CHP17 txhua tus tau qhia meej txog kev coj noj coj ua thiab kev ntsuas kom haum.

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Ib Suav Tshuaj Tshuaj ntsuab Extract Lable uas siv hauv Asmeskas kev ua lag luam

2.1 cov kab mob bile-resistant gram-tsis zoo

Nyob rau hauv pretreaticment, tsis zoo li lwm cov tshuaj pab txheem tshuaj uas siv rau lub ntsej muag nruab nrab yog usp43 ntxiv pbs ua rau diluent. Txawm hais tias qhov yuav tsum tau ua rau cov qauv rov qab lub sijhawm yog txawv me ntsis hauv txhua lub chaw muag tshuaj, lawv txhua tus yuav tsum tau rov zoo rau tsawg kawg 2 h. Hauv cov kab mob txhawb nqa theem, EP1 {0}}}}!}}}.}.}}}} ntawm cov kab mob bile-resistant gram-tsis muaj kab mob uas tuaj yeem kuaj pom los ntawm cov qauv no ncav tau mus txog 1 × 104 cfu / m (lossis cfu / ml).
Piv nrog txoj kev ntawm lwm cov tshuaj pillectureias siv 3 xeem qauv inoculation concentrations concentrations}. {}}}}}}}}}}}}}}}}. Cov hau kev siv hauv kev xaiv sib cais ntawm ntau yam tshuaj phapacopoias muaj kev sib cais, thiab nruab nrab yog siv los txheeb xyuas cov kab lis kev cai txawv, tab sis lub sijhawm sib cav thiab lub sijhawm sib txawv me ntsis.

cistanche specification

Contanche Cov Ntawv Qhia Tshwj Xeeb

2.2 tus me nyuam coli escherichia

Ep1 {{0. {}}}}}} {{0} 0 Tsuas yog coj los ntawm cov kev ntsuam xyuas kev ntsuas. Nyob rau hauv txoj kev sim siab, kev paub txog cov ntsiab cai ntawm txhua lub chaw muag tshuaj yog xwm yeem. Qhov kev xeem daws yog enriched nrog TSB nruab nrab, inoculated rau hauv macculated rau Macconkey kua nruab nrab, thiab tom qab ntawd sib cais nrog kev xaiv nruab nrab. Ntawm lawv, usp43 siv maccankey agar nruab nrab rau kev sib cais thiab kev coj noj coj ua rau 18 mus rau 24 teev, thiab micriethia coli cim; Lwm peb Tshuaj Phaj Phaj Phaj JP17 ntxiv tau ntxiv siv Escherichia coli chromogenic Agar Nruab Nrab Hloov Macconkey Agar Nruab Nrab thiab kev coj noj coj ua. Nyob rau hauv cov txheej txheem xeem xeem, ob ep1 {{}}}}}. 0001 g (lossis 0.001 g (lossis 0.01, thiab 0.01, thiab 0.01 g (lossis 0.01

2.3 salmonella

Nyob rau hauv cov nqe lus ntawm cov qauv ntsuas, tsuas yog EP1 {1}}}} Kuv yuav tsum tau ntxiv rau TSB nruab nrab. Nyob rau hauv cov kab mob xaiv cov kab mob ntxiv, USP43, EP1 {10}}}} ± 0.5) degree kom muaj kev xaiv tau zoo. Hauv theem sib cais, USP43 tuaj yeem xaiv ib lossis ntau ntawm BGA, xld lossis nws nruab nrab; ELEP10.0 thiab CHP2020 tsuas yog xav tau kev siv XLD nruab nrab, thiab JP17 tseem tuaj yeem siv Salmonella Chromogenic Agromogenic Agar Nruab Nrab. Hauv kev txheeb xyuas cov kab mob txawv txawv, USP43 ntxaij cov kab mob los ntawm kev sim peb-qab zib; Ep10.0 thiab JP17 siv txoj kev tsim nyog los txiav txim; CHP2020 sib xyaw ua ke rau ob txoj kev qhia saum toj no, uas hloov tau yooj yim dua thiab tsim nyog.

2.4 Lwm cov kab mob tswj hwm

JP17 thiab USP43 ob qho tib si hais qhia txoj kev sim rau Staphylococcus aureus, thaum EP1 {3}}. 0 thiab CHP2020 tsis qhia cov khoom no. JP17 cov thwj tim uas tau kuaj sim yog kev coj ua hauv TSB nruab nrab, nws yuav tsum tau pauv mus rau cov kab lis kev cai uas inhibit tom qab; USP43 tsuas yog siv TSB nruab nrab rau ib tus kab mob ntxiv. Thaum sib cais xaiv kab lis kev cai xov xwm tawm, jP17 thiab USP43 Siv cov vj nruab nrab thiab bp nruab nrab ntxiv rau MS nruab nrab. Thaum paub txog cov hlua khi tsis txaus ntseeg, USP43 siv ntshav cowbulase xeem, thaum JP17 tuaj yeem siv txoj hauv kev tsim nyog rau kev txheeb xyuas qhov tseeb. Ib qho ntxiv, USP43 tseem muab txoj kev pom kom pom kev zoo, uas yog tib yam li cov khoom lag luam microbiological nyob hauv usp43 "62".

Labs

Ib Suav Tshuaj Tshuaj Tshuaj ntsuab Extract HPLC sim kuaj

3 microbiological txwv cov qauv

3.1 ep1 {0. 0

Ep1 {{0
Rau chav kawm cov qauv, tus ntsuas txwv rau tamc suav yog 1 × 107 CLU / lossis CFU / lossis CFU / G (lossis CFU / lossis CL); Nyob rau tib lub sijhawm, cov nyiaj tau los ntau tshaj plaws yog tsim rau TAMC suav rau cov khoom lag luam yog 1 × 102 CFU / ML). Tsuas yog rau chav kawm cov qauv, uas tsis tas yuav raug txheeb xyuas rau cov kab mob bile-resistant gram-tsis muaj kev sib tw thiab bile-resistant gram-tsis muaj kev sib tw thiab bline-resistant gram-tsis muaj kev sib tw thiab bler-resistant gram-tsis muaj kab mob thiab bileon

3.2 jp17

JP17 "G4" Muab faib cov tshuaj Herbal thiab lawv cov ntawv rho tawm nrog cov dej npau npau (chav kawm uas tau siv ncaj qha tsis tas yuav muaj kev rho tawm (chav kawm II) [11]. Hauv kev sib piv, Chav Kawm hauv JP17 yog sib npaug rau cov kawm hauv EP1 {}} zoo ib yam li chav kawm c hauv ep1 {}}}. 0. Txawm li cas los xij, JP17 tsis qhia txog cov qauv txwv rau cov B Cov khoom hauv Ep10.0, saib rooj 3 rau cov ntsiab lus.

3.3 USP43

Piv nrog rau kev faib tawm ntawm decoction pieces hauv Ep1 {}}}}}}}}}}}}}}}}}}}}}}}. Muaj 7 pawg, nrog rau cov nroj tsuag lossis hauv av los sis sib npaug ntawm 1, thiab CFU / lossis CFUICY ib 10 g. Qhov no yuav tsum yog siab dua li qhov yuav tsum tau ntawm Ep10.0 thiab JP17 rau cov khoom lag luam ntawm tib qho kev faib tawm.

3.4 CHP2020

Raws li lub 2 0} 15 tsab cai, CHP2020 "Reveres Suav Herbal tshuaj, thiab ntxiv cov kev cai rau cov lus qhia cuav ntawm Suav, thiab ntxiv rau cov kev cai rau cov khoom siv tamp thiab ntxiv rau cov khoom noj thiab Eschreshia coli. Tsuas yog cov xim tymc suav tau ntawm 1 × 103 cfu / g, tus so yog tib yam li cov qauv microbial rau hauv EP10.0 (saib rooj 3). Txawm li cas los xij, CHP2020 tseem tsis tau tsim cov qauv txwv tsis sib haum xeeb rau cov kev tswj hwm cov kab mob tshuaj xyuas cov tshuaj ntsuab Suav. Nws tuaj yeem pom tau tias cov cai ntawm CHP2020 rau cov tshuaj hauv cov tshuaj ntsuab suav, txawm tias nws yog ua tiav raws li lwm peb pharmacopeias.

4 kev sib tham

Qhov 2 {0} 15 ib tsab ntawm cov tshuaj suav suav tau suav nrog cov tshuaj uas suav cov tshuaj tiv thaiv kab mob Txawm li cas los xij, cov txheej txheem qib kev tsim thiab cov txuj ci tag nrho Cov me me sib kis tseem muaj nyob hauv cov khoom lag luam, thiab cov kab ke ntawm cov kab mob yuav muaj nyob nrog [16-18]. Txawm hais tias feem ntau Suav tshuaj ntsuab slices yuav tsum tau ua los ntawm kev siv tshuaj, thiab lwm yam kev pheej hmoo ua ntej siv, tseem muaj kev phom sij rau cov neeg mob [}} 17-19]. Tam sim no, txawm hais tias CHP2020 tau kho dua "Microbial txwv tus qauv rau cov tshuaj ntsuab Suav thiab cov khoom tshuaj xyuas thiab cov qauv tshuaj xyuas yuav tsum tau ua ntxiv.

Suav tshuaj ntsuab yog thawj cov khoom lag luam thiab cov tshuaj tshwj xeeb. Qhov kev ua haujlwm ntawm lawv txoj kev soj ntsuam yog qhov txuas tseem ceeb hauv cov txheej txheem ntawm cov txheej txheem txheem [14]. Cov tshuaj kis tau ntawm cov tshuaj ntsuab ntawm Suav cov tshuaj ntsuab muaj siab, uas muaj kev cuam tshuam nrog kev tshuaj xyuas cov kab mob thiab muaj kev pheej hmoo ntawm kev pom [17]. Muaj ntau qhov sib txawv ntawm cov kab mob muaj txiaj ntsig thiab sib cais ntawm cov qauv kev kuaj xyuas sib txawv. Noj cov tshuaj ntsuam ntawm Staphylococcus aureus hauv JP17 ua piv txwv ntawm cov qauv kev txhim kho ntawm cov kab mob thiab txhim kho kev kuaj pom ntawm cov kab mob ntawm lub hom phiaj. USP43 thiab JP17 kuj ntxiv kev siv ntau yam xim Colorimetric kab lis kev cai xov xwm tawm, uas yog cov qauv zoo rau cov hom kab mob uas muaj qhov sib txawv. Tsis tas li ntawd, tag nrho plaub lub rooj tshuaj pharmacopoias cia cov kev kuaj mob microbial thiab cov tshuaj ntsuam xyuas roj ntsha, thiab tuaj yeem ua lub luag haujlwm soj ntsuam ntawm cov tshuaj tua kab ntawm Suav cov tshuaj ntsuab thiab Txhim kho tus nqi kuaj pom ntawm cov kab mob tswj hauv tshuaj ntsuab [{{}]. Hais txog cov qauv txwv, USP43 muaj cov tshuaj ntsuam ntawm Suav thiab cov cai txwv tsis pub rau cov qauv microbial cov tshuaj pleev cov tshuaj suav. Txawm hais tias USP43 tsis qhia meej hom kev tsis tsim nyog, nws yuav tsum tswj cov kab mob uas tsis tsim nyog, tshwj xeeb los tswj hwm cov kab mob tsis tsim nyog uas yuav ua rau muaj kev phom sij rau cov khoom lag luam thiab ua rau muaj kev nyab xeeb rau kev nyab xeeb rau cov khoom thiab muaj kev phom sij [12]. Cov tswv yim no ntawm kev ntsuas kev pheej hmoo thiab tswj kev pheej hmoo yog tsim nyog kawm los ntawm thiab xa mus rau hauv kuv lub tebchaws. Hauv cov ntsiab lus, qhov kev kawm no piv thiab txheeb xyuas cov txheej txheem microbial cov txheej txheem ntawm cov qauv tshuaj suav cov tshuaj suav hauv plaub lub tsev muag tshuaj. Raws li cov xwm txheej tam sim no ntawm microbial paug thiab tswj hwm cov ntaub ntawv hauv microbial, thiab kev tswj hwm cov tshuaj suav suav los ntxiv kom muaj kev nyab xeeb ntawm cov khoom lag luam Suav tshuaj. Nws raug nquahu tias raws li cov xwm txheej tsis zoo ntawm cov microbial thiab tswj cov qauv tshuaj suav suav yuav tsum tau txhim kho cov khoom lag luam hauv Suav teb yuav tsum tau ua kom zoo.

A Chinese Medicine Herb Regulation in USA

Ntawv Sawv cev

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