Anti-nociceptive Thiab Anti-inflammatory Kev Ua Los Ntawm Cistanche Deserticola hauv Rodents
Mar 19, 2022
Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791
Li-Wi Lin, et al
Abstract
Hauv kev tshawb nrhiav tam sim no, rhizomes ntawmCistanchedeserticola(Orobanchaceae, abbreviated li CD) tau muab rho tawm nrog 50 feem pua ethanol thiab cais tawm los ntawm ethyl acetate, n-butanol, thiab dej. Cov analgesic thiabtiv thaiv-mobcov teebmeem ntawm CD extract thiab peb txheej tau soj ntsuam nyob rau hauv ntau yam tsiaj qauv. CD(Cistanchedeserticola)extracts tau zoo inhibited writhing teb induced los ntawm 1 feem pua acetic acid thiab biphasic licking cov lus teb los ntawm 1 feem pua formalin, thiab kuj txo cov edema induced los ntawm 1 feem pua carrageenan tab sis tsis zymosan. Tsis tas li ntawd, cov txheej txheem butanoic thiab aqueous ntawm CD(Cistanchedeserticola)extract tsis tsuas yog txo qhov mob vim yog acetic acid thiab formalin tab sis kuj txo cov edema induced los ntawm carrageenan. Cov teebmeem ntawm butanoic txheej ntawm CD(Cistanchedeserticola)extract yog zoo dua li ntawm cov aqueous txheej. Tsis tas li ntawd, cov nyhuv ntawm butanoic txheej ntawm CD(Cistanchedeserticola)Extract tsis tau tshem tawm los ntawm naloxone. Cov txiaj ntsig no tau qhia tias muaj kev mob analgesic thiabtiv thaiv-mobCov teebmeem, thiab cov txheej txheem butanoic thiab aqueous yog cov tseem ceeb tshaj plaws. Tsis tas li ntawd, analgesic thiabtiv thaiv-mobcov teebmeem ntawm butanoic txheej ntawm CD(Cistanchedeserticola)extract tsis cuam tshuam rau opioid receptors thiab lub cev tiv thaiv kab mob.
Ntsiab lus:Cistanche deserticola; Kev ua haujlwm analgesic; Anti-inflammatory kev ua
1. Taw qhia
CistanchedeserticolaMa. (Orobanchaceae, abbreviated li CD), ib hom tshuaj ntsuab hauv Suav teb tau siv dav hauv cov tshuaj ib txwm siv rau ntau yam kev kho mob suav nrog sedative, analgesic, thiab kev tiv thaiv kab mob (Lee, 1986). Cov kev tshawb fawb pharmacological niaj hnub taw qhia CD(Cistanchedeserticola)extract muaj Immuno enhancing (Suav thiab Su, 1993), dawb radical scavenging nyhuv (Hsu li al., 1995), thiab nce SOD kev ua (Chang li al., 1995). Hauv kev tshawb fawb tsis ntev los no, cov tshuaj tiv thaiv dawb radical tau ua lub luag haujlwm tseem ceeb hauv cov txheej txheem inflammatory (Arrigoni-Martelli, 1985). Yog li, qhov kev tshawb fawb tam sim no tau sim los tshawb xyuas cov tshuaj tua kab mob ntawm CD(Cistanchedeserticola)extract hauv nas nrog acetic acid-induced writhing test (Taber et al., 1969) thiab formalin-induced licking test (Shibata et al., 1989). Qhov thib ob, peb kuj tau kawm txog qhov ua tautiv thaiv-mobkev ua si ntawm CD(Cistanchedeserticola)extract ntawm cov txheej txheem classical inflammatory nrog carrageenan-induced edema test (Lub caij ntuj no li al., 1962) thiab lwm txoj kev inflammatory txheej txheem nrog zymosan-induced edema test (Tarayre li al., 1989). Thaum kawg, peb tshawb fawb cov txheej txheem ntawm CD rho tawm nrog cov kuab tshuaj sib txawv. '
2. Cov ntaub ntawv thiab cov txheej txheem
2.1. Cog cov khoom thiab cov extracts
Rhizomes ntawm CD(Cistanchedeserticola)tau yuav los ntawm Taiwan kev ua lag luam thiab lees paub los ntawm tus xibfwb MT Hsieh. Ib daim ntawv pov thawj (tsis yog. CMC-ICPS-000626) tau khaws cia nyob rau hauv lub herbarium ntawm lub koom haum ntawm Suav Pharmaceutical Sciences, Tuam Tshoj Medical College rau yav tom ntej siv. Kev rho tawm thiab cais tawm ntawm CD(Cistanchedeserticola)tau ua raws li cov ntaub ntawv dhau los (Lu, 1998). CD(Cistanchedeserticola)extract (cov tawm los hauv 34.2 feem pua) thiab peb txheej * / ethyl acetate txheej, butanolic txheej thiab aqueous txheej (lawv yields nyob rau hauv 3.4, 10.2, 30.6 feem pua) (abbreviated li CDE, CDB, thiab CDW, feem) * / tau siv rau kev npaj ntawm extracts.
2.2. Kev npaj ntawm extracts thiab tshuaj
CD(Cistanchedeserticola)extract ({{0}}.1, 0.3, 1 g/kg) thiab peb txheej CD(Cistanchedeserticola)extracts ({{0}}}03, 0.1, 0.3 g/kg) tau yaj nyob rau hauv 0.5 feem pua carboxymethylcellulose thiab muab tshuaj rau qhov ncauj 60 feeb ua ntej rau kev txhaj tshuaj ntawm inducer. Acetylsalicylic acid (ASA) (100, 300 mg / kg) thiab indomethacin (IN DOL) (10 mg / kg) tau npaj raws li kev ncua nrog 0.5 feem pua carboxymethylcellulose thiab muab tshuaj intraperitoneally ua ntej txhaj tshuaj inducer. Naloxone (1 mg / kg) yog yaj nrog ib txwm saline.
2.3. Tsiaj
Txiv neej Sprague-Dawley nas (200-250 g) tau siv rau kev kawm ntawmtiv thaiv-mobkev ua si. Txiv neej ICR nas (20-25 g) tau siv los kuaj cov tshuaj tua kab mob. Tag nrho cov tsiaj txhu tau siv rau hauv kev sim ua raws li Txoj Cai Qhia Txog Kev Saib Xyuas thiab Kev Siv Cov Tsiaj Txhaum Cai. Lawv tau nyob hauv tsawg kawg yog 1 lub lis piam ua ntej pib qhov kev sim hauv 239 / 1 8C kub thiab 60 feem pua ntawm cov av noo tswj ib puag ncig nrog kev nkag mus rau cov khoom noj pub dawb hauv pellets (muab thiab tsim los ntawm Fwusow Industry Co. LTD., Taiwan ) thiab kais dej, nyob rau hauv ib tug 12-12 h lub teeb / tsaus voj voog (lub teeb theem: 08:00-20:00 h) tau khaws cia. Tom qab ntawd, cov tshuaj tau muab tshuaj thiab cov tshuaj analgesic thiabtiv thaiv-mobkev soj ntsuam tau ua los ntawm ob txoj kev dig muag. Tom qab ntsuas tus cwj pwm, txhua tus tsiaj raug tua los ntawm carbon dioxide.
2.4. Kev tshawb nrhiav Antinociceptive
2.4.1. Acetic acid-induced plab writhing teb nyob rau hauv nas
Cov nas tau muab faib ua ob peb pawg. Txhua tus nas tau muab 1 feem pua ntawm cov tshuaj acetic acid (10 ml / kg lub cev hnyav). Cov nas tau muab tso rau hauv lub thawv soj ntsuam tus kheej. Cov tsos mob ntawm acetic acid-vim plab plab writhing zoo ib yam li cov lus piav qhia los ntawm Taber li al. (1969). Tswj cov tsiaj tau txais kev daws teeb meem tsheb hauv tib qhov kev sim. Tsib feeb tom qab txhaj tshuaj acetic acid, tus naj npawb ntawm writhing teb rau ib tus nas raug suav rau 10 min thaum lub sij hawm acetic acid-induced plab writhing. Thaum kawg, tus naj npawb ntawm writhing teb tau tso cai rau peb qhia txog qhov feem pua ntawm kev tiv thaiv siv cov piv txwv hauv qab no: (tswj txhais tau tias kho tau) / 100 / tswj txhais tau tias.
2.4.2. Formalin-induced licking teb nyob rau hauv nas
Txoj kev no sawv cev rau kev hloov kho uas tau piav qhia los ntawm Shibata li al. (1989). Txhua tus nas tau muab tso rau hauv qhov chaw soj ntsuam ntawm ib qho acrylic pob tshab phaj pem teb rau 5 feeb ua ntej txhaj tshuaj formalin. Hauv qab hauv pem teb, ib daim iav loj yog inclined ntawm 458 lub kaum sab xis thiaj li tso cai rau pom tseeb ntawm paws ntawm tus tsiaj. Cov tsiaj tau muab 25 ml ntawm 1 feem pua formalin rau hauv txoj cai subplantar. Tom qab ntawd, txhua tus tsiaj raug xa rov qab mus rau chav tsev thiab ob lub sijhawm sib txawv ntawm cov lus teb licking hnyav tau pom. Thawj lub sijhawm (thaum ntxov) tau sau tseg 0 /5 min tom qab txhaj tshuaj formalin thiab lub sijhawm thib ob (qib lig) tau sau tseg 10 / 35 min tom qab txhaj tshuaj. Lub sij hawm (s) siv nyob rau hauv licking cov lus teb ntawm qhov txhaj tshuaj paw yog ntsuas raws li qhov qhia ntawm qhov mob teb.
2.5. Anti-inflammatory kev kawm nyob rau hauv nas
Covtiv thaiv-mobKev ua haujlwm tau txiav txim siab hauv cov nas los ntawm kev ntsuas qhov nruab nrab ntawm qhov nce hauv cov paw ntim tom qab sub plantar txhaj tshuaj ntawm cov kab mob xws li carrageenan (Lub caij ntuj no li al., 1962) lossis zymosan (Tarayre li al., 1989). Cov tsiaj tau txhaj nrog 0.1 ml 1 feem pua carrageenan lossis 2 feem pua zymosan nyob rau hauv txoj cai hind ko taw nyob rau hauv lub plantar aponeurosis. Qhov mob tau suav nrog cov milliliters siv lub plethysmometer (7150 Ugo Basile), uas tau sau tseg qhov sib txawv me me ntawm cov dej los ntawm qhov ntim tawm. Ua ntej ib qho kev kho mob, qhov nruab nrab ntawm lub nraub qaum ntawm txhua tus tsiaj tau txiav txim siab (Vo) nrog peb qhov kev ntsuas uas tsis txawv ntawm ntau tshaj 4 feem pua (qhov tseeb ntawm cov cuab yeej). Tom qab ntawd 30, 60, 90, 120, 150, 180, 210, 240 min tom qab txhaj tshuaj tiv thaiv kab mob, qhov nruab nrab ntim ntawm lub nraub qaum ntawm txhua tus tsiaj tau txiav txim siab (Vt) nrog peb qhov ntsuas uas tsis txawv ntawm ntau tshaj. 4 feem pua.
Qhov feem pua ntawm edema ntawm txhua cov ntaub ntawv tau suav los ntawm kev sib piv qhov nruab nrab ntawm lub nraub qaum ntawm txhua tus tsiaj (Vt) tom qab txhaj tshuaj ntawm cov kab mob inflammatory nrog qhov nruab nrab ntim ntawm lub nraub qaum ntawm txhua tus tsiaj (Vo) ua ntej kev kho mob (Palanichamy thiab Nagarajan, 1990). Feem pua ntawm inhibition tau txais rau txhua pawg los ntawm kev siv cov piv txwv hauv qab no: [(Vt-Vo) tswj /(Vt-Vo) kho]-100/ (Vt-Vo) tswj.
2.6. Kev txheeb cais
Tag nrho cov ntaub ntawv tau txais thaum lub sij hawm analgesic thiabtiv thaiv-mobkev ua haujlwm tau hais tawm hauv cov ntsiab lus ntawm qhov nruab nrab thiab cov qauv tsis raug thiab soj ntsuam ntxiv los ntawm kev siv ANOVA ib txoj kev tsom xam ntawm qhov sib txawv, ua raws li Scheff qhov kev xeem. Thaum qhov tshwm sim (P) tsawg dua 0.05, qhov sib txawv tau suav tias yog qhov tseem ceeb.
3. Cov txiaj ntsig thiab kev sib tham
Cov tsiaj pretreatment nrog qhov ncauj koob tshuaj CD(Cistanchedeserticola)rho tawm (0.1-1 g/kg) ua rau muaj kev noj tshuaj raws li kev noj tshuaj thiab tseem ceeb inhibition ntawm acetic acid-induced writhing teb (Table 1) thiab cov theem biphasic ntawm formalin-induced licking teb nyob rau hauv nas ( Fig. 1(A)). Qhov inhibition tshwm sim los ntawm CD(Cistanchedeserticola)rho tawm ntawm 1 g / kg ntawm acetic acid-induced writhing teb thiab formalin-induced licking teb yog hais txog 54 thiab 45 feem pua, feem. Qhov zoo tswj acetylsalicylic acid ntawm 0.1-0.3 g/kg kuj txo cov acetic acid-induced writhing teb nrog inhibition ntawm 45-64 feem pua (Table 1). Txawm li cas los xij, lwm qhov kev tswj hwm zoo indomethacin ntawm 10 mg / kg tsuas yog inhibited lig tab sis tsis yog theem pib ntawm formalin-induced licking teb nyob rau hauv nas nrog qhov siab tshaj plaws inhibition ntawm 32 feem pua (Fig. 1(A)). Tsis tas li ntawd, tsuas yog butanolic thiab aqueous txheej ntawm peb txheej ntawm CD(Cistanchedeserticola)rho tawm ntawm 0.1-0.3 g/kg kuj tau tsim cov tshuaj tiv thaiv tseem ceeb, thiab cov tshuaj tua kab mob ntawm cov txheej txheem butanolic tau zoo dua li cov txheej txheem dej (Table 1 thiab Fig. 1(B)) . Qhov thib ob, peb ntsuas qhovtiv thaiv-mobkev ua haujlwm ntawm CD(Cistanchedeserticola)extract thiab peb txheej tiv thaiv classical (carrageenan-induced) thiab lwm yam (zymosan-induced) edema tsim. Peb nrhiav tau CD(Cistanchedeserticola)extract ntawm 0.1-1g/kg kuj tseem inhibited carrageenan-induced edema tsim nyob rau hauv ib tug npaum li cas ntawm cov tshuaj thiab indomethacin ntawm 10 mg/kg kuj zoo inhibited carrageenan-induced edema tsim nyob rau hauv nas (Fig. 2). Txawm li cas los xij, zymosan-induced edema tsim tsis tau inhibited los ntawm CD(Cistanchedeserticola)rho tawm ntawm 0 1-1 g/kg hauv nas (cov ntaub ntawv tsis qhia). Tsis tas li ntawd, tsuas yog txheej butanolic ntawm peb txheej ntawm CD(Cistanchedeserticola)extract ntawm 0.1-0.3 g/kg kuj tseem inhibited carrageenan-induced edema tsim nyob rau hauv nas (Fig. 2). Raws li phytochemical cov ntaub ntawv, acteoside thiab succinic acid, teej tug mus rau cov khoom xyaw ntawm butanolic txheej ntawm CD.(Cistanchedeserticola)extract (Hsu et al., 1994, 1995), muaj covtiv thaiv-mobKev ua haujlwm ntawm cov tshuaj lom neeg tsim tawm (Murai li al., 1995; Kabachnyi li al., 1987). Los ntawm peb cov ntaub ntawv tam sim no thiab lwm cov ntaub ntawv dhau los, peb tau pom zoo rhizomes ntawm CD(Cistanchedeserticola)muaj analgesic thiabtiv thaiv-mobKev ua haujlwm hauv nas, thiab cov khoom siv nquag tawm hauv cov txheej txheem butanolic ntawm CD.(Cistanchedeserticola)rho tawm. Seb acteoside thiab succinic acid yog cov khoom xyaw nquag ntawm CD(Cistanchedeserticola), peb yuav cais thiab soj ntsuam lawv cov analgesic thiabtiv thaiv-mobkev ua si yav tom ntej.
Fig. 1. Cov nyhuv ntawm CD(Cistanchedeserticola)extract ({0}}.1, 0.3 thiab 1 g / kg), ethyl acetate, butanolic thiab aqueous txheej ntawm CD(Cistanchedeserticola)extract (CDE, CDB thiab CDW) ({{0}}}03, 0.1 thiab 0.3 g/kg) thiab indomethacin (INDOL, 10 mg/kg) thaum ntxov (0 / 5 min) thiab theem lig (10 / 35 min) ntawm formalin-induced licking teb nyob rau hauv nas. Txhua tus nqi yog sawv cev raws li mean9/SE (N / 7). *PB/0.05, *** PB/0.001, piv nrog rau pawg VEH.
Fig. 2. Cov nyhuv ntawm CD(Cistanchedeserticola)extract ({0}}.1, 0.3 thiab 1 g / kg), ethyl acetate, butanolic thiab aqueous txheej ntawm CD(Cistanchedeserticola)extract (CDE, CDB thiab CDW) ({{0}}}03, {{10}}.1 thiab 0.3 g/kg) thiab indomethacin (INDOL, 10 mg / kg) ntawm carrageenan-induced paw edema hauv nas. Txhua tus nqi yog sawv cev raws li mean9/SE (N / 6). *PB/0.05, **PB/0.01, as piv nrog VEH pab pawg
Tsis tas li ntawd, Shibata et al. (1989) qhia txog theem pib ntawm formalin-induced licking teb yog cuam tshuam nrog bradykinin thiab tshuaj P, thiab theem lig cuam tshuam nrog bradykinin, autocrine, thiab prostaglandin. Peb cov ntaub ntawv tam sim no tau taw qhia naloxone tsis tau tshem tawmtiv thaiv-mobkev ua haujlwm ntawm butanol txheej ntawm CD(Cistanchedeserticola)rho tawm ntawm ob theem ntawm formalin-induced licking teb (Daim duab 3) thiab cov nyhuv analgesic ntawm acetic acid-induced writhing teb (Table 1). Yog li ntawd, analgesic thiabtiv thaiv-mobCov teebmeem ntawm butanol txheej ntawm CD extract yuav tsis cuam tshuam rau opioid receptors. Tsis tas li ntawd, CD(Cistanchedeserticola)extract thiab nws txheej butanolic tsuas yog inhibited carrageenan-induced edema tsim nyob rau hauv nas. Carrageenan-induced edema ntawm polymorphonuclear leukocyte infiltration thiab activated kinin system tshwj xeeb tshaj yog bradykinin txawv ntawm zymosan-induced edema ntawm monocyte influx thiab activated immune system (Damas li al., 1990; Dawson et al., 1991). Succinic acid, cov khoom xyaw ntawm butanolic txheej ntawm CD extract, inhibited polymorphonuclear leukocyte muaj nuj nqi (Rotstein li al., 1985). Cov txiaj ntsig no tau qhia tias cov tshuaj analgesic thiabtiv thaiv-mobCov teebmeem ntawm butanol txheej ntawm CD(Cistanchedeserticola)extract tej zaum yuav muaj feem xyuam rau kinin system tab sis tsis opioid receptors thiab lub cev tiv thaiv kab mob txawm tias CD(Cistanchedeserticola)muaj cov tshuaj tiv thaiv kab mob (Chin and Su, 1993). Rau cov ncauj lus kom ntxaws mechanism ntawm analgesic thiabtiv thaiv-mobkev ua haujlwm, peb yuav tsum tshawb xyuas ntxiv nrog cov txheej txheem ua haujlwm lossis cov khoom xyaw ntawm CD extract.
Cistanchetubulosamuaj ntau yam teebmeem, nyem qhov no kom paub ntau ntxiv
Cov ntaub ntawv
Arrigoni-Martelli, E., 1985. Pharmacology of free radical scavenging in o. International Journal of Tissue Reactions 7, 513/519.
Chang, Y., Wu, H., Wang, SN, Cheng, HC, 1995. Kawm txog cov teebmeem muaj zog ntawmCistanchedeserticolaMa., Cistanche salsa (CA Mey) G. Beck and Cistanche tubulosa (Schenk) R Wight. Tuam Tshoj Journal of Chinese Materials and Medicine 26, 143/146.
Chin, HL, Su, YC, 1993. Kawm txog cov tshuaj pharmacological ntawmCistanchedeserticolaMa. China Journal of Chinese Materials and Medicine 19, 143/146.
Damas, J., Bourdon, V., Remacle-Volon, G., Adam, A., 1990. Kinins thiab peritoneal exudates induced by carrageenin and zymosan in nas. British Journal of Pharmacology 101, 418/422.
Dawson, J., Sedgwick, AD, Edwards, JC, Lees, P., 1991. Ib qho kev kawm sib piv ntawm cov cellular, exudative thiab histological teb rau carrageenan, dextran, thiab zymosan hauv nas. International Journal of Tissue Reactions 13, 171/185.
Hsu, WH, Chu, SH, Chao, CH, Hsu, Y., Yuan, KW, Yen, CH, Chi, CY, Chan, C., 1994. Study on the chemical constituents ofCistanchedeserticolaMa. Tuam Tshoj Phau ntawv Journal of Chinese Materials and Medicine 25, 509/513.
Hsu, WH, Chu, SH, Sheng, LC, Yuan, KW, Mao, CN, 1995. Kawm txog cov tshuaj lom neeg thiab cov tshuaj pharmacological ntawmCistanchedeserticolaMa. thiab Cistanche salsa (CA Mey) G. Beck. Suav tshuaj ntsuab tshuaj ntsuab 26, 143/146.
Kabachnyi, VI, Chernykh, VP, Drogovoz, SM, Golubenko, Iu.A., Bogutskaia, EE, 1987. Search fortiv thaiv-mobKev npaj ntawm cov derivatives ntawm succinic acid heteryl amides. Farmakologiia thiab Toksikologiia 50, 94/97.
Lee, SL, 1986. Pen-Tsao-Kun-Mu. National Research Institute of Chinese Medicine Publications, Taipei, p. 20.
Lu, MC, 1998. Studies on the sedative effect ofCistanchedeserticola. Phau ntawv Journal of Ethnopharmacology 59, 161/165.
Murai, M., Tamayama, Y., Nishibe, S., 1995. Phenylethanoids nyob rau hauv cov tshuaj ntsuab ntawm Plantago lanceolata thiab inhibitory nyhuv ntawm arachidonic acid-induced nas pob ntseg edema. Planta Medica 61, 479/480.
Palanichamy, S., Nagarajan, S., 1990. Kev ua kom mob plab ntawm Cassia alata leat extract thiab kemp Eerol 3-0-Sophoroside. Phau ntawv Journal of Ethnopharmacology 29, 73/78.
Rotstein, OD, Pruett, TL, Fiegel, VD, Nelson, RD, Simmons, RL, 1985. Succinic acid, metabolic by-product of Bacteroides species, inhibits polymorphonuclear leukocyte function. Kab mob thiab tiv thaiv kab mob 48, 402/408.
Shibata, M., Ohkubo, T., Takahashi, H., Inoki, R., 1989. Kev hloov kho formalin test: yam ntxwv mob biphasic teb. 38, 347/352.
Taber, RI, Greenhouse, DD, Rendell, JK, Irwin, S., 1969. Agonist thiab antagonist kev sib cuam tshuam ntawm opioids ntawm acetic acid-induced plab stretching hauv nas. Phau ntawv Journal of Pharmacology and Experimental Therapeutics 169, 29/38.
Tarayre, JP, Delhon, A., Aliaga, M., Barbara, M., Bruniquel, F., Caillol, V., Puech, L., Consul, N., Tisne-Versailles, J., 1989.
Pharmacological kev tshawb fawb ntawm zymosan o nyob rau hauv nas thiab nas. 1: Zymosan-induced paw edema nyob rau hauv nas thiab nas. Pharmacological Research 21, 375/384.
Winter, CA, Risley, EA, Nuss, GW, 1962. Carrageenan-induced edema in hind paw of the nas assay fortiv thaiv-mobtshuaj. Proceedings Society of Experimental Biology and Medicine 111, 544/547.
