Abstract

Keeb kwm:Platelet aggregation thiab qib siab glycation kawg cov khoom (AGEs) thiab oxidative kev nyuaj siab yog lub npe hu ua yam tseem ceeb rau kev loj hlob ntawm cov kab mob plawv thiab mob ntshav qab zib. Nyob rau hauv cov ntsiab lus no, txiv hmab txiv ntoo thiab zaub noj, qhov zoo ntawm cov tshuaj tiv thaiv kab mob antioxidant tau tshaj tawm tias yog ib txoj hauv kev zoo los tiv thaiv tib neeg los ntawm cov kab mob no. Txoj kev tshawb fawb tam sim no tsom mus rau kev ntsuam xyuas cov tshuaj tiv thaiv antioxidant, antiplatelet, thiab kev tiv thaiv glycation ntawm pomegranate (Punica granatum L.) paj (PF), nplooj (PL), tev (PP) kua txiv (PJ), thiab noob roj (PSO) .

Glycoside ntawm cistanche tuaj yeem ua rau muaj kev ua haujlwm ntawm SOD hauv plawv thiab daim siab cov ntaub so ntswg, thiab txo cov ntsiab lus ntawm lipofuscin thiab MDA hauv txhua cov ntaub so ntswg, tshem tawm ntau yam reactive oxygen radicals (OH-, H₂O₂, thiab lwm yam) thiab tiv thaiv DNA puas. los ntawm OH-radicals. Cistanche phenylethanoid glycosides muaj peev xwm tshem tawm cov dawb radicals, muaj peev xwm txo tau ntau dua li cov vitamin C, txhim kho cov haujlwm ntawm SOD hauv cov phev ncua, txo cov ntsiab lus ntawm MDA, thiab muaj qee yam kev tiv thaiv ntawm cov phev ua haujlwm. Cistanche polysaccharides tuaj yeem txhim kho kev ua haujlwm ntawm SOD thiab GSH-Px hauv erythrocytes thiab ntsws cov ntaub so ntswg ntawm cov nas senescent sim los ntawm D-galactose, nrog rau txo cov ntsiab lus ntawm MDA thiab collagen hauv lub ntsws thiab ntshav, thiab nce cov ntsiab lus ntawm elastin, muaj Cov nyhuv scavenging zoo ntawm DPPH, ncua lub sijhawm ntawm hypoxia hauv cov nas senescent, txhim kho cov haujlwm ntawm SOD hauv cov ntshav, thiab ncua lub physiological degeneration ntawm lub ntsws hauv kev sim cov nas nrog cellular morphological degeneration, kev sim tau pom tias Cistanche muaj peev xwm antioxidant zoo. thiab muaj peev xwm los ua ib qho tshuaj tiv thaiv thiab kho cov kab mob ntawm daim tawv nqaij laus. Nyob rau tib lub sijhawm, echinacoside hauv Cistanche muaj lub peev xwm tseem ceeb los tshem tawm DPPH dawb radicals thiab tuaj yeem tshem tawm cov pa oxygen reactive, tiv thaiv dawb radical-induced collagen degradation, thiab kuj muaj kev kho zoo ntawm thymine dawb radical anion puas.

Nyem rau ntawm Cistanche thiab Tongkat Ali Reddit

【Yog xav paub ntxiv: david.deng@wecistanche.com / WhatApp:86 13632399501 】

Cov txheej txheem: Antioxidant kev ua ub no tau ntsuas tawm tsam ABTS radical thiab lipid peroxidation. Kev ua haujlwm Antiglycation tau txiav txim siab siv qhov tsim ntawm AGE fluorescence siv hauv BSA / ribose system. Kev ua haujlwm antiplatelet tau ntsuas hauv platelet-nplua nuj plasma (PRP) tiv thaiv adenosine diphosphate (ADP), Collagen, thiab arachidonic acid (AA).

Cov txiaj ntsig:PF extract tau pom qhov ua haujlwm zoo tshaj plaws antioxidant tiv thaiv ABTS thiab lipid peroxidation nrog IC50 qhov tseem ceeb ntawm 0.7mg/mL thiab 0.63mg/mL feem. Rau kev ua haujlwm los tiv thaiv glycation, PP, PF, thiab PL inhibited nruab nrab ntawm pentosidine-zoo li AGEs tsim piv rau kev tswj zoo nrog AGE-IC50 tus nqi ntawm 0.4mg / mL. PJ thiab PSO tsis muaj kev tiv thaiv AGE. Tag nrho cov extracts xaiv inhibited platelet aggregation tshwm sim los ntawm ib, ob, los yog peb inducers nyob rau hauv ib koob tshuaj-nyob ntawm seb. PF yog qhov muaj zog tshaj plaws inhibitor tshwm sim los ntawm tag nrho peb inducers, nrog inhibitory cuam tshuam los ntawm 35.6 mus rau 66.6 feem pua. PP thiab PJ tau nthuav tawm cov nyhuv antiplatelet tawm tsam ADP thiab collagen thiab PL thiab PSO nkaus xwb tawm tsam AA.

Cov lus xaus:Cov txiaj ntsig no qhia tias qee cov pomegranate extracts muaj peev xwm hauv vitro antiglycation thiab antiplatelet kev ua ub no

Ntsiab lus:Pomegranate, Antiplatelet kev ua, Advanced glycation kawg-cov khoom, Oxidative stress, Lipid peroxidation

Keeb kwm

Pomegranate (Punica granatum L.), tau dav paub tias yog cov txiv hmab txiv ntoo muaj zog antioxidant [1–3]. Lub zog antioxidant ntawm cov kua txiv pomegranate tau raug tshaj tawm tias yog 3-fold siab dua li ntawm cov cawv liab lossis tshuaj yej ntsuab [4] thiab 8-fold ntau dua li cov uas tau pom hauv cov kua txiv kab ntxwv [5]. Tsis tas li ntawd, ib qho txiv hmab txiv ntoo uas muaj nyob rau hauv kev tshawb fawb ntau yog pomegranate thiab nws cov ntsiab lus uas tau tshaj tawm tias muaj zog lom thiab muaj txiaj ntsig tshuaj. Pomegranate kua txiv, tev, noob roj, nplooj, thiab paj rho tawm tau piav qhia kom muaj nyob rau hauv vitro nrog rau hauv vivo antidiabetic [6], anti-inflammatory [7], antioxidant, anti-obesity [8], thiab antitumor teebmeem [ 9]. Cov txiaj ntsig zoo no muaj feem xyuam rau qhov muaj cov tshuaj tiv thaiv kab mob siab heev xws li polyphenolic tebchaw, suav nrog hydrolyzable tannins, anthocyanins, thiab flavonols [10]. Hauv peb cov kev tshawb fawb yav dhau los ntawm cov tshuaj tiv thaiv kab mob ntshav qab zib ntawm pomegranate, cov txiaj ntsig tau qhia txog cov txiaj ntsig neuroprotective ntawm pomegranate extracts thiab qhia tau hais tias kev noj zaub mov ntev ntawm pomegranate tuaj yeem yog lwm txoj hauv kev rau kev tiv thaiv HFD (High Fat High Fructose Diet) ntxias cov insulin. tsis kam thiab oxidative kev nyuaj siab [6, 11]. Pomegranate kua txiv, nplooj, thiab tev noj tau ua rau txo qis hauv cov ntshav qabzib ceev ceev thiab qib insulin. Yog li ntawd, qhov homeostatic Performance index ntawm insulin tsis kam (HOMA-IR) uas yog siv los ntsuas cov tshuaj insulin kuj raug txo qis uas qhia tias muaj kev txhim kho tseem ceeb hauv insulin rhiab heev.

Hauv cov ntsiab lus no, peb tau sim ntsuas cov txiaj ntsig ntawm pomegranate extracts tawm tsam cov teeb meem ntshav qab zib uas paub tshaj plaws xws li platelet aggregation thiab Advanced glycation kawg khoom (AGEs) uas tau tshaj tawm tias muaj feem cuam tshuam nrog kev mob ntshav qab zib thiab kev laus [12, 13] . Qhov inhibition ntawm platelet muaj nuj nqi tau txais los ua ib lub tswv yim los kho mob vascular atherothrombotic kab mob xws li mob ntshav qab zib mob plawv, thiab ischemic stroke [14, 15]. Advanced glycation kawg cov khoom (AGEs) yog txuam nrog kev pheej hmoo ntawm kev mob ntshav qab zib ntau dua xws li ntshav qab zib retinopathy, neuropathy, thiab nephropathy [16]. Tsis tas li ntawd, ob peb tsab ntawv tshaj tawm tau tshaj tawm txog kev cuam tshuam ntawm cov ntoo pomegranate sib txawv ntawm AGE tsim [17] lossis platelet aggregation [18]. Hauv txoj haujlwm no, peb tau tshawb xyuas hauv vitro anti-AGE thiab antiplatelet muaj peev xwm thiab qee qhov kev ua haujlwm antioxidant ntawm pomegranate kua txiv (PJ), tev (PP), paj (PF), nplooj (PL), thiab noob roj (PSO).

Cov txheej txheem

Cog cov ntaub ntawv thiab extraction

Nplooj thiab txiv hmab txiv ntoo tau sau los ntawm Tounsi pomegranate ntoo thaum Lub Kaum Hli 2021 los ntawm thaj av Mahdia, Tunisia. Ntau yam authenticity tau lees paub los ntawm taxonomist Dr. Faten Zaouay los ntawm Department of Horticulture, Higher Agronomic Institute, Chott-Meriem (University of Sousse, Tunisia), thiab ib daim ntawv pov thawj tau muab tso rau hauv peb lub teb chaws sau khaws cia hauv duplicate ntawm Gabes thiab Chott-Mariem. (Sousse), nrog rau code 'TNl, TN2, TN3, TN5, TN5".

Pomegranate extracts tau npaj raws li tau piav qhia los ntawm peb txoj kev tshawb fawb dhau los [11]. Txiv hmab txiv ntoo raug ntxuav thiab ntxuav tes. Arils tau nyem siv lub tshuab ua lag luam (Moulinex, Fabkis). Cov kua txiv extracted tau centrifuged ntawm 15000rpm rau 15min. Tom qab ntawd tus supernatant tau rov kho dua thiab lyophilized. Nplooj, paj, thiab txiv hmab txiv ntoo tev tau qhuav, hmoov, thiab muab rho tawm nrog methanol (MeOH) 50g / 250ml nyob rau hauv qhov tsaus ntuj rau 48 teev. Txhua lub extract tau lim los ntawm Whatman No. 42 lim ntawv thiab evaporated rau dryness siv lub tshuab rotary evaporator (Heidolph, Lub teb chaws Yelemees) nyob rau hauv lub tshuab nqus tsev ntawm 45 degree thiab khaws cia ntawm −20 degree rau kev txiav txim ntxiv. Pomegranate noob tau qhuav thiab hmoov. Roj tau muab rho tawm los ntawm txoj kev ntawm soxhlet. Txog 30g cov noob tau muab rho tawm nrog 200 ml ntawm hexane ntawm chav tsev kub rau 6h. Cov kuab tshuaj raug tshem tawm los ntawm evaporation ntawm 40 degree thiab cov roj tau ntws nrog cov dej nitrogen thiab khaws cia ntawm -20 degree hauv cov raj kaw.

ABTS radical scavenging assay

Lub peev xwm antioxidant ntawm pomegranate extracts los ntawm ABTS (2,2′-casino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) assay tau ntsuas los ntawm txoj kev dhau los [19]. luv luv, ABTS• ntxiv radical Cov tshuaj yog tsim los ntawm reacting ABTS Tshuag tov (5mM) nrog poov tshuaj persulfate (K2S2O8) tov (2.7mM). pH 7.4) kom tau txais qhov nqus ntawm 0.700±0.020 ntawm 660nm. Kaum, ABTS• ntxiv rau cov tshuaj tau tov nrog pomegranate extracts npaj ntawm qhov sib txawv. Tom qab incubation, lub absorbance yog ntsuas ntawm 734nm. Qhov feem pua ​​ntawm inhibition ntawm ABTS• ntxiv radical tau suav nrog cov qauv hauv qab no:

Acontrol hais txog cov tshuaj uas muaj cov ntshiab MeOH es tsis txhob siv cov qauv, thiab ib qho piv txwv hais txog qhov absorbance ntawm pomegranate extract-muaj cov kev daws teeb meem. Qhov kev ua tau zoo ntawm cov qauv yog tsim nyog los txo qhov nqus ABTS• ntxiv rau 50 feem pua ​​(EC50) tau txiav txim siab.

Lipid peroxidation siv txoj kev ferric thiocyanate

Qhov inhibition ntawm lipid peroxidation los ntawm pomegranate extracts tau soj ntsuam raws li cov txheej txheem dhau los [20]. Linoleic acid (LA) tau siv los ua lipid matrix thiab 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) ua cov dawb radical pib. Qhov sib txawv ntawm txhua qhov pomegranate extract tau npaj. Txhua qhov kev xav tau sib xyaw nrog 1.3 feem pua ​​(w/v) methanolic LA thiab 0.2M phosphate tsis (pH 7.0) thiab cov peroxidation tau pib los ntawm kev sib ntxiv ntawm AAPH tov (55.3mM) hauv phosphate tsis. Cov tshuaj tswj tau npaj los ntawm kev ntxiv cov ntshiab MeOH es tsis txhob siv cov qauv. Tom qab incubation ntawm 50 degree rau 24h nyob rau hauv qhov tsaus ntuj, cov tshuaj tiv thaiv sib tov yog yaj nyob rau hauv ib tug 3: 1 (v / v) H2O-MeOH tov. Kaum, ib qho 10 feem pua ​​aqueous tov ntawm NH4SCN thiab 20 mM FeCl2 hauv 3.5 feem pua ​​HCl tau ntxiv. Tom qab 3min ntawm incubation nyob rau hauv chav tsev kub, lub absorbance yog ntsuas ntawm 546nm tiv thaiv cov khoom seem. Ascorbic acid tau siv los ua kev tswj xyuas zoo. Cov txiaj ntsig tau qhia raws li feem pua ​​​​ntawm lipid peroxidation inhibition:

Tswjhais txog cov tshuaj uas muaj cov ntshiab MeOH es tsis txhob siv cov qauv, thiab cov qauv yog hais txog cov absorbance ntawm cov roj uas muaj cov tshuaj. Te EC50 tau txiav txim siab.

Advanced glycation end-products inhibition assay

Inhibition ntawm pentosidine-zoo li AGEs tsim thiab EC50 qhov tseem ceeb tau txiav txim siab thiab suav nrog siv txoj kev piav qhia yav dhau los los ntawm Séro et al. 2013, nrog kev hloov kho me ntsis [21]. Luv luv, BSA (10mg / mL) tau incubated nrog D-ribose (0.5M) ua ke nrog cov tshuaj ntsuam xyuas hauv 50 mM phosphate tsis nyob ntawm pH 7.4 (NaN3, 0.02 feem pua). Cov kev daws teeb meem tau tsim nyob rau hauv 96-zoo microtiter daim hlau ntawm 37 degree rau 24h hauv lub kaw lus kaw ua ntej AGE fluorescence ntsuas. Fluorescence tshwm sim los ntawm incubation, nyob rau hauv tib BSA (10mg / mL) thiab kuaj extract tej yam kev mob, raug rho tawm rau txhua qhov kev ntsuas. Pentosidine-zoo li (λexc 335nm, λem 385nm) AGEs fluorescence tau ntsuas siv microplate spectrofluorometer. Qhov feem pua ​​​​ntawm AGEs tsim tau raug xam raws li hauv qab no rau txhua qhov kev tawm dag zog thiab EC50 qhov tseem ceeb tau txiav txim siab:

AGEs (%)=[fluorescence intensity (piv txwv) − fluorescence intensity (blank of the sample)] ∗100/[fluorescence intensity (tswj) − fluorescence intensity (tsis muaj kev tswj)]

Kev ntsuam xyuas hauv vitro ntawm kev tiv thaiv platelet aggregation ua haujlwm

Cov ntshav tshiab tau txais los ntawm cov neeg ua haujlwm noj qab haus huv uas muaj keeb kwm tsis zoo ntawm kev noj tshuaj, dej haus, lossis khoom noj uas yuav cuam tshuam rau kev sib sau ua ke tsawg kawg 10 hnub thiab zoo dua yuav tsum tau yoo mov thaum hmo ntuj vim tias muaj chylomicron tuaj yeem cuam tshuam cov qauv sib sau. Txoj kev tshawb no tau pom zoo los ntawm pawg neeg saib xyuas kev ncaj ncees hauv nroog ntawm University Hospital Hedi Chaker ntawm Sfax, Tunisia.

Cov ntshav venous tau sau rau hauv ib lub raj yas uas muaj trisodium citrate 109mM. PRP tau txais los ntawm centrifuging ntawm chav tsev kub rau 12min ntawm 200 × g. PRP raug tshem tawm kom zoo kom tsis txhob kis kab mob nrog cov qe ntshav liab lossis lub tsho buffy, thiab khaws cia hauv chav sov kom txog thaum kuaj. Txhua qhov kev xeem yuav tsum ua kom tiav hauv 3 teev tom qab npaj PRP. Tom qab ntawd cov ntshav ntxiv tau centrifuged ntawm 2000 × g rau 20min kom tau txais cov ntshav tsis zoo (PPP). Peb siv cov tshuaj ntsuam xyuas ntawm cov neeg sawv cev sib sau ua ke: adenosine 5'-diphosphate (ADP, 20μM), collagen (5ug / mL), thiab arachidonic acid (2mM).

PRP thiab PPP tau siv los teeb tsa, raws li, 0 thiab 100 feem pua ​​​​lub teeb kis tau tus mob hauv qhov ntsuas ntsuas. Platelet aggregation tau saib xyuas tsawg kawg 5 feeb tom qab ntxiv cov agonist.

Rau pomegranate nplooj (PL), paj (PF), kua txiv hmab txiv ntoo (PJ), thiab tev (PP) extracts, sib txawv concentrations tau npaj yav tas los rau txhua extract yaj nyob rau hauv DMSO (ntawm {{0}}}.05 feem pua ​​zaum kawg concentration). Rau PSO, ntau qhov sib txawv tau yaj hauv 70 feem pua ​​​​polyethylene glycol (PEG) uas yog siv dav hauv vivo kom yaj cov dej-insoluble compounds. Kaum microliters ntawm txhua cov extract tau ntxiv rau 260μL ntawm kev tswj PRP, thiab tom qab ntawd cov sib tov tau incubated rau tsawg kawg yog 5 feeb (txog 30min) ntawm 37 degree ua ntej ntxiv agonists. Kaum collagen (5ug / mL), AA (2mM), lossis ADP (20μmol / L) tau ntxiv thiab platelet zoo li hloov thiab sib sau ua ke tau saib xyuas rau 5 feeb. DMSO (0.5 feem pua ​​​​v / v) tau siv los tswj qhov tsis zoo thiab cov tshuaj aspirin tau siv los tswj qhov zoo.

D=Platelet aggregation nyob rau hauv lub xub ntiag ntawm cov tshuaj ntsuam xyuas

S=platelet aggregation nyob rau hauv muaj cov kuab tshuaj.

Cov platelet aggregation inhibitory kev ua haujlwm tau qhia tias feem pua ​​inhibition los ntawm kev sib piv nrog qhov ntsuas rau lub tsheb (DMSO lossis PEG) ib leeg. Txhua tus qauv raug ntsuas hauv triplicate thiab cov ntaub ntawv tau nthuav tawm raws li qhov ntsuas ± SD. Cov txiaj ntsig ntawm kev ua tau zoo xav tau rau 50 feem pua ​​​​ inhibition ntawm platelet aggregation (EC50), tau txais los ntawm tsawg kawg peb qhov kev txiav txim.

Kev txheeb cais

Cov txiaj ntsig tau qhia raws li qhov txhais tau tias tsawg kawg peb qhov kev ntsuas kev ywj pheej, tshwj tsis yog cov qauv sib txawv tau tshaj tawm (txhais tau tias ± SD) thiab tshuaj xyuas siv SPSS ver. 21.0, kev tshaj tawm. Rau kev ua haujlwm antioxidant, Duncan qhov kev sim tau siv los kwv yees qhov tseem ceeb ntawm cov teebmeem tseem ceeb ntawm 5 feem pua ​​​​qhov yuav tshwm sim (P<0.05).

Cov txiaj ntsig thiab kev sib tham

Antioxidant proprieties ntawm pomegranate extracts

Lub peev xwm antioxidant ntawm pomegranate extracts tau ntsuas los ntawm ABTS thiab lipid peroxidation kev soj ntsuam. Cov txiaj ntsig tau muab sau tseg rau hauv Table 1 thiab tau nthuav tawm raws li EC50 tus nqi. qis EC5{{10}}} qhia tias muaj kev ua haujlwm antioxidant ntau dua. Nws tau pom tias cov extracts txawv ntawm ib leeg nyob rau hauv cov nqe lus ntawm lawv cov teebmeem antioxidant. Piv txwv li, PF tau nthuav tawm qhov ua haujlwm antioxidant siab tshaj plaws tiv thaiv ABTS nrog EC50 qhov tseem ceeb ntawm 0.7 mg / ml, zoo dua txawm tias tus qauv ascorbic acid uas muaj IC50 ntawm 1.4 mg / ml. PF pom qhov thib ob qis tshaj EC50 rau lipid peroxidation assay (0.63 mg / mL), me ntsis loj dua tus qauv ascorbic acid (0.52 mg / mL), txawm li cas los xij, qhov sib txawv no tsis tseem ceeb (p <0.05). PP extract ua raws li PL thiab PJ rho tawm tuaj yeem txo cov dawb radical ABTS. Tib qhov kev txiav txim tau pom nyob rau hauv kev kuaj lipid peroxidation. Txawm li cas los xij, PSO tau pom qhov tsis muaj zog antioxidant kev ua haujlwm hauv ob qho tib si hauv vitro kev soj ntsuam. Nws tau tshaj tawm tias muaj kev sib raug zoo ntawm cov ntsiab lus phenolic thiab lub peev xwm antioxidant [22]. Hauv peb txoj kev tshawb fawb yav dhau los [23], peb tau kawm txog cov ntsiab lus phenolic ntawm pomegranate paj, nplooj, tev, thiab kua txiv hmab txiv ntoo thiab peb piv lawv cov kev txo hwj chim thiab kev tiv thaiv DPPH. Cov txiaj ntsig tau pom tias txhua lub hauv nruab nrog cev kuj tseem muaj txiaj ntsig txo lub zog thiab kev ua haujlwm antiradical. Paj thiab nplooj tau nplua nuj nyob rau hauv phenols thiab ua pov thawj tias yog cov muaj zog antioxidants.

Anti-AGEs muaj peev xwm ntawm pomegranate extracts

The anti-glycation capacities of pomegranate extracts evaluated by their inhibition of the formation of global fluorescent AGEs in the BSA/ribose system are depicted in Fig. 1 and Table 2. PP, PF, and PL extracts demonstrated a dose-response inhibition of the pentosidine-like AGEs formation (Fig. 1) with an AGE-EC50 value of 0.4mg/ml  (Table 2). This anti-AGEs capacity is considered moderate compared to that exhibited by Aminoguanidine (AGEEC50; 0.16-0.17mg/mL) and weak compared to Rutoside trihydrate (AGE-EC50; 0.05mg/mL). However, results show that PJ and PSO haven't any anti-AGE effect (AGEEC50; >1 mg / ml). Hauv kev tshawb nrhiav ob qhov muag tsis pom, Sohrab (2015) tau xaus lus tias pomegranate (Punica granatum) kua txiv txo qis lipid peroxidation, tab sis tsis muaj kev cuam tshuam rau cov ntshav siab glycated kawg cov khoom hauv cov neeg laus uas muaj ntshav qab zib hom 2 [24]. Peb cov txiaj ntsig ntsig txog cov kua txiv pomegranate tsis yog raws li qee qhov kev tshawb pom yav dhau los qhia los ntawm Liu (2014), uas pom tias pomegranate txiv hmab txiv ntoo extract (PFE) pom muaj zog tiv thaiv glycation [25]. Cov kev ua haujlwm los tiv thaiv glycation ntawm cov sib txawv pomegranate rho tawm tuaj yeem raug ntaus nqi rau lawv cov phenolic constituents. Kumagai (2015), tau qhia tias AGEs tsim tau los ntawm BSA nrog piam thaj, fructose, thiab glyceraldehyde hauv vitro yog concentration-dependently suppressed los ntawm kev ntxiv ntawm pomegranate txiv hmab txiv ntoo extract PFE thiab nws cov phenolic Cheebtsam xws li punicalin, punicalagin, ellagic acid, thiab gallic. acid [17].

Antiplatelet kev ua haujlwm ntawm pomegranate extracts

Pomegranate qhov chaw raug soj ntsuam rau lawv lub peev xwm los inhibit platelet aggregation ntawm tib neeg PRP induced los ntawm ADP, Collagen, thiab AA li muaj zog aggregation inducers. Table 3 qhia txog qhov inhibitory cuam tshuam ntawm cov extracts sib txawv ntawm ntau qhov ntau thiab cov tshuaj aspirin raws li kev tswj tau zoo thiab Table 4 tau sau tseg EC50 qhov tseem ceeb ntawm pomegranate extracts lossis cov tebchaw nrog qhov txiaj ntsig ntawm peb qhov kev ntsuas. Tag nrho cov extracts xaiv inhibited platelet aggregation tshwm sim los ntawm ib, ob, los yog peb inducers nyob rau hauv ib koob tshuaj-nyob ntawm seb.

Paj extract tau pom tias yog qhov muaj zog tshaj plaws inhibitor ntawm platelet aggregation tshwm sim los ntawm tag nrho peb inducers, nrog inhibitory los ntawm 35.6 mus rau 66.6 feem pua ​​ntawm 3.5 mg / mL. Nws tau nquag tawm tsam collagen-induced platelet aggregation nrog EC50 tus nqi ntawm 2.8 mg / mL, tom qab ntawd tawm tsam AA-induced platelet aggregation nrog EC50 tus nqi ntawm 3.85 mg / mL thiab nrog 4. {15}} mg/mL thaum sib sau ua ke los ntawm ADP. Muab piv rau Aspirin raws li kev tswj hwm zoo, PF, PP, thiab PJ muaj kev cuam tshuam rau kev sib sau ua ke los ntawm collagen. Txawm li cas los xij, Aspirin inhibited aggregation induced los ntawm AA thiab ADP nrog EC50 ntawm 0.42 thiab 0.66 mg / ml feem tab sis tsis pom muaj txiaj ntsig tiv thaiv collagen. Hauv txoj kev tshawb no thiab peb txoj kev tshawb fawb yav dhau los [23], PF tau pom tias yog cov tshuaj tiv thaiv antioxidant tshaj plaws tiv thaiv DPPH radical, ABTS radical, thiab lipid peroxidation piv rau PP, PL, thiab PJ. Qhov kev tshawb pom no yuav piav qhia tias yog vim li cas PF yog qhov zoo tshaj plaws inhibitor ntawm platelet aggregation. Tsis tas li ntawd, PF yog nplua nuj nyob rau hauv phenols (16.6 feem pua) nrog rau feem ntau hydrolyzed tannins (ellagitannin) thiab soluble noj fiber ntau (30.2 feem pua) [26]. Hydrolyzed tannins tau dhau los ua pov thawj tias muaj txiaj ntsig zoo hauv inhibiting platelet muaj nuj nqi [18]. Ntawm qhov tod tes, kev ua haujlwm antiplatelet ntawm kev noj haus fiber ntau yog ntub dej tsis paub tseeb [27, 28]. Yog li, peb xav tias lub zog thiab ntau lub hom phiaj antiplatelet kev ua haujlwm ntawm PF tuaj yeem raug ntaus nqi rau hydrolyzed tannins, loj phenols pom hauv lub cev.

PP thiab PJ nthuav tawm cov teebmeem inhibitor tawm tsam ADP thiab collagen-induced platelet aggregation. Txawm li cas los xij, tsis muaj kev cuam tshuam rau ob qho tib si rho tawm thaum AA tau siv los ua agonist. Peb cov txiaj ntsig tsis lees paub tias pom los ntawm Mattiello li al., 2009 uas qhia tias ob qho tib si rho tawm inhibit platelet teb rau AA [18]. Kev sib piv ntawm EC50 qhov tseem ceeb tau qhia tias PP txo qis ADP thiab collagen-induced platelet aggregation zoo dua PJ.

Qhov sib txawv ntawm inhibitory nyhuv ntawm ob qho tib si rho tawm tuaj yeem piav qhia los ntawm qhov sib txawv ntawm qhov muaj peev xwm antioxidant. PP yog ib qho muaj zog antioxidant tshaj PJ tawm tsam ABTS radical thiab lipid peroxidation hauv txoj kev tshawb no thiab tseem tiv thaiv DPPH radical thiab txo lub zog [23]. Qhov kev piav qhia no yog qhov tsis sib haum xeeb nrog qee cov ntawv tshaj tawm yav dhau los uas tau qhia tias cov tshuaj tiv thaiv kab mob ntawm cov txiv hmab txiv ntoo zoo li tsis cuam tshuam lossis cuam tshuam rau lawv cov haujlwm antioxidant [29, 30]. PL thiab PSO muaj peev xwm inhibit ADP-triggered platelet aggregation thaum lawv tsis zoo thaum collagen thiab AA tau siv los ua agonists.

Xaus

Hauv kev xaus, pomegranate paj, nplooj, thiab tev muaj nyob rau hauv vitro inhibitory cuam tshuam rau protein glycation thiab platelet aggregation. Cov teebmeem no tau raug ntaus nqi los ntawm cov khoom siv antioxidant ntawm ntau pomegranate active compounds. Txawm li cas los xij, kev tshawb fawb ntxiv yog tsim nyog los lees paub cov txiaj ntsig no thiab kom tau txais kev nkag siab tob dua ntawm nws cov txheej txheem ntawm kev ua haujlwm, ua ntej tau thov ua AGE inhibitor. Cov cuab yeej antioxidant? Cov tshuaj nquag hauv pomegranate muaj peev xwm / pab txhawb rau cov khoom no.

Cov ntawv luv

AA: arachidonic acid; AAPH: 2,2′-azobis (2-methylpropionamidine) dihydrochloride; ABTS: 2,2′-casino-bis (3-ethylbenzothiazoline-6-sulfonic acid; ADP: Adenosine Diphosphate (ADP); AGEs: Advanced Glycation end products; BSA: Bovin Serum Albumin; DMSO: Dimethyl Sulfoxide FeCl2: Ferrous chloride; HCl: Chlorhydric acid; HFD: High Fat High Fructose Diet; K2S2O8: Potassium persulfate solution; LA: Linoleic acid; MeOH: Methanol; NaN3: Sodium azide; NH4SCN: Ammonium: thiocyanateene PEG; Platelet-nplua nuj plasma; PPP: Platelet pluag plasma.

Kev lees paub

Cov lus qhia txog tsob ntoo

Ntau yam authenticity tau lees paub los ntawm taxonomist Dr. Faten Zaouay los ntawm Department of Horticulture, Higher Agronomic Institute, Chott-Meriem (University of Sousse, Tunisia), thiab daim ntawv pov thawj tau muab tso rau hauv peb lub teb chaws sau khaws cia hauv duplicate ntawm Gabes thiab Chott-Mariem. (Sousse), nrog rau cov cai 'TNl, TN2, TN3, TN5, TN5". Horticulture thiab Organic Chott-Mariem, IRESA-University of Sousse, 8.P.57-4042, Tunisia.

Cov neeg sau ntawv pab txhawb

ZA thiab IA; methodology, ZA; thiab IA; software, AZ, thiab RC; validation, cov peev txheej, ZA; kev sau ntawv-kev npaj ua ntej, JG; kev saib xyuas, MH, thiab SH; sau ntawv- tshuaj xyuas thiab kho. Txhua tus kws sau ntawv tau nyeem thiab pom zoo rau cov ntawv luam tawm ntawm cov ntawv sau.

Nyiaj txiag

Txoj haujlwm no tau txais nyiaj los ntawm Ministry of Higher Education thiab Kev Tshawb Fawb Txog Kev Tshawb Fawb-Tunisia.

Muaj cov ntaub ntawv thiab cov ntaub ntawv

Cov ntaub ntawv tsim tawm thaum lub sijhawm thiab / lossis tshuaj xyuas thaum lub sijhawm kawm tam sim no muaj los ntawm tus kws sau ntawv raws li qhov kev thov tsim nyog.

Cov lus tshaj tawm

Kev pom zoo ntawm kev ncaj ncees thiab kev tso cai los koom

Txoj kev tshawb no tau pom zoo los ntawm Pawg Saib Xyuas Kev Ncaj Ncees hauv cheeb tsam ntawm University Tsev Kho Mob Hedi Chaker ntawm Sfax, Tunisia. Txhua qhov kev sim tau ua los ntawm cov lus qhia thiab cov kev cai cuam tshuam. Cov ntaub ntawv tso cai tau txais los ntawm txhua yam kev kawm thiab / lossis lawv tus neeg saib xyuas raug cai.

Tso cai rau kev tshaj tawm

Tsis siv tau.

Kev nyiam sib tw

Cov kws sau ntawv tshaj tawm tsis muaj kev sib cav txog kev txaus siab.

Sau Paub meej

1 Biochemistry Laboratory, LR12ES05 "Nutrition- Functional Foods and vascular Health", Kws Qhia Ntawv ntawm Tshuaj, University of Monastir, 5019 Monastir, Tunisia. 2 Center Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia.

Cov ntaub ntawv

1. Hanafy SM, Abd El-Shafea YM, Saleh WD, Fathy HM. Tshuaj profiling, nyob rau hauv vitro antimicrobial thiab antioxidant kev ua ub no ntawm pomegranate, txiv kab ntxwv thiab txiv tsawb tev extracts tawm tsam pathogenic microorganisms. J Genet Eng Biotechnol. 2021; 19:80 Nws.

2. Benchagra L, Berrougui H, Islam MO, Ramchoun M, Boulbaroud S, Hajjaji A, et al. Antioxidant nyhuv ntawm Moroccan pomegranate (Punica granatum L. Sefri ntau yam) extracts nplua nuj nyob rau hauv Punicalagin tiv thaiv oxidative kev nyuaj siab. Khoom noj khoom haus. 2021; 10: 2219.

3. Akuru EA, Chukwuma CI, Oyeagu CE, Erukainure OL, Mashile B, Setlhodi R, et al. Khoom noj khoom haus thiab phytochemical profile ntawm pomegranate ("zoo heev ntau yam") tev thiab nws cov teebmeem ntawm hepatic oxidative stress thiab metabolic alterations. J Food Biochem. 2022:46 ib.

4. Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant kev ua ntawm pomegranate kua txiv thiab nws cov kev sib raug zoo nrog phenolic muaj pes tsawg leeg thiab ua. J Agric Food Chem. 2000; 48:4581–9.

5. Rosenblat M, Hayek T, Aviram M. Anti-oxidative teebmeem ntawm pomegranate kua txiv (PJ) noj los ntawm cov neeg mob ntshav qab zib ntawm cov ntshav qab zib thiab ntawm macrophages. Atherosclerosis. 2006; 187:363–71.

6. Amri Z, Ben Khedher MR, Zaibi MS, Kharroubi W, Turki M, Ayadi F, et al. Anti-diabetic teebmeem ntawm pomegranate extracts nyob rau hauv lub sij hawm ntev siab fructose rog-fed nas. Clin Phytoscience. Xyoo 2020; 6:55.

7. Harzallah A, Hammami M, Kępczyńska MA, Hislop DC, Arch JRS, Cawthorne MA, et al. Kev sib piv ntawm cov kev tiv thaiv muaj peev xwm ntawm pomegranate paj, tev, thiab noob roj ntawm insulin tsis kam thiab o ntawm cov rog thiab siab-sucrose noj-vim cov nas rog rog. Arch Physiol Biochem. 2016; 122:75–87.

8. Al-Muammar MN, Khan F. Obesity: kev tiv thaiv lub luag hauj lwm ntawm pomegranate (Punica granatum). Khoom noj khoom haus. 2012; 28:595–604.

9. Amri Z, Kharroubi W, Fidanzi-Dugas C, Leger DY, Hammami M, Liagre B. Kev loj hlob inhibitory thiab pro-apoptotic cuam tshuam ntawm ornamental pomegranate extracts hauv Du145 tib neeg prostate cancer hlwb. Nutr Cancer. 2020; 72:932–8.

10. Arlotta C, Puglia GD, Genovese C, Toscano V, Karlova R, Beekwilder J, et al. MYB5-zoo li thiab bHLH cuam tshuam flavonoid muaj pes tsawg leeg hauv pomegranate. Tsob Ntoo Sci. Xyoo 2020; 298: 110563.

11. Amri Z, Ghorbel A, Turki M, Akrout FM, Ayadi F, Elfeki A, thiab al. Cov txiaj ntsig ntawm pomegranate extracts ntawm lub hlwb cov cim antioxidant thiab cholinesterase kev ua haujlwm hauv cov rog-siab fructose noj-vim kev rog rog hauv cov qauv nas. BMC Complement Altern Med. Xyoo 2017; 17:339.

12. Okura T, Ueta E, Nakamura R, Fujioka Y, Sumi K, Matsumoto K, et al. Cov khoom lag luam qib siab glycation kawg yog txuam nrog kev txo qis ntawm cov tshuaj insulin hauv cov neeg mob ntshav qab zib hom 2: daim ntawv qhia luv luv. J Diabetes Res. Xyoo 2017; 2017.

13. Al-Sofani ME, Yanek LR, Faraday N, Kral BG, Mathias R, Becker LC, et al. Ntshav qab zib thiab platelet teb rau cov tshuaj aspirin qis. J Clin Endocrinol Metab. 2018; 103: 4599–608.

14. McEwen BJ. Kev cuam tshuam ntawm kev noj zaub mov thiab cov as-ham ntawm platelet muaj nuj nqi. Semin Thromb Hemost. 2014; 40:214–26.

15. Lim ST, Coughlan CA, Murphy SJX, Fernandez-Cadenas I, Montaner J, Thijs V, et al. Platelet function testing in transient ischemic attack thiab ischemic stroke: ib qho kev tshuaj xyuas zoo ntawm cov ntaub ntawv. Platelets. 2015; 26:402–12.

16. Dhananjayan K, Forbes J, Munch G. Advanced Glycation, Ntshav Qab Zib, thiab Dementia. Hauv: Hom 2 Ntshav Qab Zib thiab Dementia; 2018. p. 169–93 : kuv.

17. Kumagai Y, Nakatani S, Onodera H, Nagatomo A, Nishida N, Matsuura Y, et al. Anti-glycation los ntawm pomegranate (Punica granatum L.) txiv hmab txiv ntoo extract thiab nws cov khoom hauv vivo thiab hauv vitro. J Agric Food Chem. 2015; 63:7760–4.

18. Mattiello T, Trifrò E, Jotti GS, Pulcinelli FM. Cov teebmeem ntawm cov kua txiv pomegranate thiab extract polyphenols ntawm platelet muaj nuj nqi. J Med Food. 2009; 12:334–9.

19. Delgado-Andrade C, Morales FJ. Unraveling qhov kev koom tes ntawm melanoidins rau kev ua haujlwm antioxidant ntawm kas fes brews. J Agric Food Chem. 2005; 53:1403–7.

20. Olszewska MA, Presler A, Michel P. Profiling ntawm phenolic tebchaw thiab antioxidant kev ua ntawm qhuav extracts los ntawm cov xaiv Sorbus hom. Molecules. 2012; 17:3093–113.

21. Séro L, Sanguinet L, Blanchard P, Dang BT, Morel S, Richomme P, et al. Tuning ib 96-zoo microtiter phaj fluorescence-raws li kev ntsuam xyuas los txheeb xyuas AGE inhibitors hauv cov nroj tsuag nyoos. Molecules. 2013; 18:14320–39.

22. Piluzza G, Bullitta S. Kev sib raug zoo ntawm cov ntsiab lus phenolic thiab antioxidant zog nyob rau hauv nees nkaum plaub hom nroj tsuag ntawm ib txwm siv ethnoveterinary nyob rau hauv cheeb tsam Mediterranean. Pharm Biol. 2011; 49:240–7.

23. Amri Z, Zaouay F, Lazreg-Aref H, Soltana H, Mneri A, Mars M, et al. Phytochemical cov ntsiab lus, fatty acids muaj pes tsawg leeg thiab antioxidant muaj peev xwm ntawm txawv pomegranate qhov chaw: sib piv ntawm edible thiab nonedible ntau yam zus nyob rau hauv Tunisia. Int J Biol Macromol. 2017; 104: 274–80.

24. Sohrab G, Angoorani P, Tohidi M, Tabibi H, Kimiagar M, Nasrollahzadeh J. Pomegranate (Punicagranatum) kua txiv txo qis lipid peroxidation, tab sis tsis cuam tshuam rau plasma qib siab glycated kawg-cov khoom hauv cov neeg laus uas muaj ntshav qab zib hom 2: randomized double- qhov muag tsis pom kev kuaj sim. Khoom noj khoom haus. Nutr Res. Xyoo 2015: 59.

25. Liu W, Ma H, Frost L, Yuan T, Dain JA, Seeram NP. Pomegranate phenolics inhibit qhov tsim ntawm qib siab glycation endproducts los ntawm scavenging reactive carbonyl hom. Food Funct. 2014; 5:2996–3004.

26. Aviram M, Volkova N, Coleman R, Dreher M, Reddy MK, Ferreira D, et al. Pomegranate phenolics los ntawm tev, arils, thiab paj yog antiatherogenic: kev tshawb fawb hauv vivo hauv atherosclerotic apolipoprotein E-deficient (E0) nas thiab hauv vitro hauv kab lis kev cai macrophages thiab lipoproteins. Phau ntawv Journal of Agricultural thiab Food Chemistry. 2008; 56:1148–57.

27. Hannan JMA, Rokeya B, Faruque O, Nahar N, Mosihuzzaman M, Azad Khan AK, et al. Cov txiaj ntsig ntawm kev noj zaub mov muaj fiber ntau ntawm Trigonella foenum graecum ntawm glycemic, insulinemic, lipidemic, thiab platelet aggregation txheej xwm ntawm hom 2 mob ntshav qab zib qauv nas. J Ethnopharmacol. 2003; 88:73–7.

28. Bagger M, Andersen O, Nielsen JB, Ryttig KR. Kev noj zaub mov fiber ntau txo cov ntshav siab, cov roj cholesterol tag nrho, thiab platelet aggregation hauv nas. Br J Nutr. 1996; 75:483–93.

29. Dutta-Roy AK, Gordon MJ, Kelly C, Hunter K, Crosbie L, Knight-Carpentar T, et al. Inhibitory nyhuv ntawm Ginkgo biloba extracts ntawm tib neeg platelet aggregation. Platelets. 1999; 10:298–305.

30. Dutta-Roy AK, Crosbie L, Gordon MJ. Cov teebmeem ntawm lws suav extract ntawm tib neeg platelet aggregation hauv vitro. Platelets. 2001; 12:218–27.

Publisher's Note 

Springer Nature tseem nyob nruab nrab ntawm kev txiav txim plaub ntug hauv daim duab qhia kev tshaj tawm thiab cov koom haum koom nrog.

【Yog xav paub ntxiv: david.deng@wecistanche.com / WhatApp:86 13632399501 】