Ib qho Ethanolic Extract Ntawm Cucurbita Pepo L. Cov noob hloov kho Neuroendocrine cuam tshuam hauv cov nas muaj zog ntev thiab qhov kev nthuav qhia ntawm cov kab mob qog nqaij hlav thiab HSP70

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Hailah M. Almohaimeed1, Shereen Hamed2, Hanan S. Seleem 3,4, Ashwaq H. Batawi 5, Zuhair M. Mohammedsaleh6, Maha Jameal Balgoon7, Soad S. Ali 8,9, Soad Al Jaouni 9,10 thiab Nasra Ayuob11*

Keeb kwm: Taub dag(Cucurbita pepo L.) tau piav qhia tias muajantioxidant,anti-inflammatory, anti- nkees, thiab antidepressant zoo li cov teebmeem. Lub qog adrenal yog ib qho tseem ceeb ntawm kev ntxhov siab-ua lub cev uas tswj hwm homeostasis thaum muaj kev ntxhov siab.

Lub hom phiaj:Txoj kev tshawb no tsom los ntsuas qhov ua tau zoo ntawm kev tswj hwm ntawm Cucurbitapepo L. (CP) extract hauv kev txo qis kev coj tus cwj pwm, biochemical, thiab cov qauv kev hloov pauv hauv cov qog qog nqaij hlav uas raug cuam tshuam los ntawm kev cuam tshuam rau qhov tsis muaj kev ntxhov siab me me (CUMS) thiab tshawb xyuas cov txheej txheem tom qab qhov cuam tshuam no.

Cov ntaub ntawv thiab cov txheej txheem:Plaub caug txiv neej albino nas tau muab faib ua 4 pawg (n=10): tswj, CUMS, fluoxetine-kho, thiab CP-kho pab pawg. Kev hloov pauv tus cwj pwm, qib corticosterone, pro-inflammatory cytokines TNF- thiab IL-6, thiab oxidant /antioxidantprofile raug soj ntsuam hauv cov ntshav qab zib thaum kawg ntawm qhov kev sim. Adrenalglands tau ua tiav rau kev soj ntsuam histopathological thiab immunohistochemical.Gene qhia ntawm caspase-3 thiab Ki67 thiab cov protein ua rau kub hnyiab 70 (HSP70) tau soj ntsuam hauv cov qog adrenal siv RT-PCR.

Cov txiaj ntsig:CP extract txo qis qib corticosterone (p < {{0}}}.001),="" lub="" sij="" hawm="" tsis="" muaj="" zog="" (p=""><0.001), thiab="" inflammatory="" thiab="" oxidative="" hloov="" pauv="" cuam="" tshuam="" nrog="" cums-vim="" kev="" nyuaj="" siab="" piv="" rau="" pawg="" tsis="" kho.="" cp="" extract="" alleviatedcums-induced="" adrenal="" histopathological="" hloov="" pauv="" thiab="" txo="">apoptosis(p < 0.001) thiab tau txais kev tswj hwm zoo heevantioxidantqib hauv cov ntshav.

Xaus:Cucurbita pepo L. ua tau zoo ameliorated cov kev ntxhov siab ntev-vim kev coj cwj pwm, biochemical, thiab kev hloov pauv ntawm adrenal feem ntau los ntawm nws.antioxidantthiab anti-inflammatory teebmeem.

Ntsiab lus: taub dag, kev nyuaj siab, kev nyuaj siab, caspase-3, ki67, Hsp70,apoptosis

Cistanche, tshuaj ntsuab suav, muaj cov haujlwm antioxidant.

Taw qhia

Ntau tshaj 264 lab tus tib neeg ntawm txhua lub hnub nyoog raug kev nyuaj siab thoob plaws ntiaj teb (WHO, 2020). Kev ua haujlwm ntawm tus neeg muaj kev nyuaj siab feem ntau yog cov neeg pluag hauv tsev kawm ntawv, tom haujlwm, thiab hauv tsev neeg. Kev ntxhov siab yuav ua rau tua tus kheej; yog li, nws tau suav tias yog qhov ua rau tuag thib ob hauv 15- rau 29-cov hnub nyoog (WHO, 2020). Cov tsiaj nyeg tuaj yeem muaj sia nyob cov xwm txheej ntxhov siab los ntawm kev ua kom cov lus teb tsim nyog rau cov xwm txheej no. Lub qog adrenal yog ib feem ntawm lub hypothalamic-pituitary-adrenocortical (HPA) axis thiab lub siab xav-adrenomedullary axis uas tswj hwm homeostasisduring kev nyuaj siab (Ulrich-Lai li al., 2006). Nws tau tshaj tawm tias qhov kev cuam tshuam rau kev ntxhov siab ua rau muaj kev qhia sai ntawm HSP70 hauv adrenal cortex (Huang et al., 2001). Cov proteins no sib koom ua lag luam intracellular, kev nthuav qhia antigen,apoptosis, thiab ntau yam kev ua (Khar et al., 2001). Nws tau piav qhia tias cov tshuaj hormones exogenous lossis cuam tshuam nrog cov tshuaj hormones endogenous, thaum lub sijhawm tseem ceeb ntawm kev txhim kho, tuaj yeem muaj kev cuam tshuam tas mus li ntawm lub cev thiab kev coj cwj pwm txoj hauv kev tswj hwm los ntawm hypothalamicneuroendocrine circuits (Gore thiab Patisaul, 2010). suav nrog tag nrho qhov dav ntawm kev sib koom ua ke physiology; yog li ntawd, nws yog ntau tshaj li hormonal chim siab (Waye thiab Trudeau, 2011).

Kev hloov pauv hauv kev tswj hwm neuroendocrine, cov metabolism, thiab kev noj haus / microbiota yog suav tias ua rau mob thiab ua rau muaj kev nyuaj siab (Huang li al., 2019). Nws tau tshaj tawm tias kev nyuaj siab - ib qho ntawm cov kab mob neuropsychiatricdisorders - thiab qhov mob ua rau muaj kev sib raug zoo ntawm ob txoj kev.Thaum kev nyuaj siab txhawb cov kev mob tshwm sim, kev mob ua rau muaj kev puas siab puas ntsws xws li kev nyuaj siab (Bauer thiab Teixeira, 2019).

Fluoxetine, ib tug classical antidepressant, yog ib tug ntawm cov tshuaj muaj nyob rau hauv lub lag luam rau kev kho mob ntawm kev nyuaj siab. Nws yog tam sim no suav hais tias yog qhov tshwm sim neuroendocrine cuam tshuam. Qhov kev cuam tshuam ntawm fluoxetine no yog ib qho kev mob tshwm sim, tsis yog cov tshuaj kho mob tseem ceeb hauv cov tsiaj (León-Olea li al., 2014). Yog li ntawd, yuav tsum muaj kev nyab xeeb antidepressants uas tsis muaj kev phiv los ntawm qhov xwm txheej ntawm cov neuroendocrine.

Taub dag(Cucurbita pepo L.) yog cov tsiaj tseem ceeb ntawm kev cog qoob loo thoob ntiaj teb. Lawv muaj cov khoom noj khoom haus thiab kev noj qab haus huv vim tias lawv muaj phenolics, flavonoids, amino acids, carbohydrates, thiab vitamins (Wang li al., 2002). Ntau cov kev tshawb fawb tau qhia tias taub dag muaj ntau yam bioactivities, xws li tshuaj tua kab mob, tshuaj tiv thaiv kab mob,antioxidant, anti-inflammatory, anti- fatigue, thiab antidepressant-zoo li cov teebmeem (Wang et al., 2012; Zhanget al., 2012; Nawirska-Olszańska et al., 2013; Kim Nr et al., 2016). systems thiab Chinesemedicine tau siv ntau qhov chaw ntawm taub dag, nrog rau cov nqaij ntawm cov txiv hmab txiv ntoo thiab cov noob (Perez Gutierrez, 2016). Cov tshuaj tiv thaiv kev ntxhov siab ntawm Sweetme Sweet Pumpkin (SSP) thiab Cucurbita moschata Duch yav dhau los tau sim hauv ib qho hauv vivostudy siv qhov yuam kev ua luam dej (FST)-vim tus qauv ntawm kev nyuaj siab thiab tau muab piv nrog fluoxetine (Kim Nr et al., 2016). Nyob rau hauv ib qho ntawm cov kev tshuaj xyuas tsis ntev los no tau sau tseg tias cov khoom noj tshuaj tiv thaiv kev ntxhov siab, xws li cov noob taub dag, tau piav qhia kom muaj cov khoom noj muaj zog tiv thaiv kev ntxhov siab ntawm 47 feem pua, muab nws cov tshuaj tiv thaiv zoo li cov nyhuv (Lachance thiab Ramsey, 2018). Tsis ntev los no, Dotto thiab Chacha pom zoo ua ntau yam tsiaj- thiab kev soj ntsuam kev tshawb fawb-raws li kev tshawb fawb kom paub meej txog cov txiaj ntsig zoo ntawm cov noob taub dag ondepression (Dotto thiab Chacha, 2020).

Cov ntawv ceeb toom no tau txhawb kom sim qhov ua tau zoo ntawmtaub daghauv alleviating qhov cuam tshuam ntawm kev ntxhov siab ntev ntawm cov qog adrenal. Yog li, qhov kev tshawb fawb no tau ua los ntsuas qhov cuam tshuam ntawm kev tswj hwm qhov ncauj ntawm Cucurbita pepo L. (CP) rho tawm ntawm kev txo qis kev ntxhov siab me me (CUMS) - ua rau muaj kev coj cwj pwm, biochemical, thiab adrenalstructure thiab tshawb nrhiav cov txheej txheem hauv qab no.

suab puam cistanche extract: Anti-oxidation

Cov khoom siv thiab cov txheej txheem

Extraction thiab Dosage ntawm Pumpkin

Cucurbita pepo L. (voucher specimen: AQJ_95) waspurchased from the local market in Jeddah, Saudi Arabia.Lawv tau txheeb xyuas hauv King Abdulaziz Universityherbarium siv cov qauv ntawm herbarium thiab cov nroj tsuag ntawmKSA (Chaudhary, 2001). Daim ntawv pov thawj tau muab tso rau hauv herbarium. CP tau txheeb xyuas los ntawm cov kws sau ntawv thiab tau lees paub los ntawm botanist los ntawm Kws qhia ntawv ntawm Science, KingAbdulaziz University.

Kev rho tawm ntawm CP tau ua tiav raws li cov kev tshawb fawb yav dhau los (Wang li al., 2012). Cov txiv hmab txiv ntoo nyoos nrog cov tev tau txiav siv aslicer thiab qhuav siv lyophilizer (FD5508; ilShinBioBase Co., Ltd., Kauslim) thiab tom qab ntawd crushed los ntawm kev siv lub tshuab hluav taws xob sib tsoo. Cov hmoov tau dhau los ntawm 40-mesh sieve kom tau cov hmoov afine thiab khaws cia rau hauv lub thawv airtight. Cov hmoov qhuav (50 g) tau tov nrog 450 ml ntawm 80 feem pua ​​​​ethanol thiab sab laug rau 1 hnub ntawm 37 degree hauv shaker (JSSI-100T; JS Research Inc., Compact ShakingIncubator., Kauslim) thiab tom qab ntawd tau lim. nrog paj rwb thiab filterpaper rau hnub ob. Cov txheej txheem rho tawm no tau rov ua dua ob zaug ntawm 37 degree kom tau txais ethanol extract.

Kev txheeb xyuas cov ntsiab lus ntawm CP

Cov tshuaj lom neeg muaj pes tsawg leeg ntawm PE tau txheeb xyuas siv cov kab mob gaschromatography GC-TSQ evo 8000 pawg spectrometer (ThermoScientific, Austin, TX, United States) nrog cov kab ncaj qha capillarycolumn TG–5MS (30 m × 0.25 hli × 0.25 µm zaj duab xis thickness). Lub qhov cub kub tau pib rau 50 degree thiab tom qab ntawd nce 5 degree / min mus rau 250 degree thiab tuav rau 2 min thiab ces nce mus rau qhov kawg kub ntawm 300 degree los ntawm 25 degree / min thiab tuav rau 2 min. Lub injector thiab MS hloov kab kub tau khaws cia ntawm 270 thiab 260 degree, ntsig txog; helium tau siv los ua cov cab kuj ntawm qhov ntws tsis tu ncua ntawm 1 ml / min. Cov kuab tshuaj ncua yog 4 min, thiab cov qauv diluted ntawm 3 µl tau txhaj tshuaj siv Autosampler AS1300 ua ke nrog GC nyob rau hauv lub splitlessmode hauv PTV injector. EI loj spectra tau sau ntawm 70 eVionization voltages tshaj qhov ntau ntawm m / z 50-650 nyob rau hauv tag nrho scanmode. Qhov kub ntawm ion tau teem rau ntawm 250 degree. Cov ntsiab lus tau txheeb xyuas los ntawm kev sib piv ntawm lawv cov massspectra nrog cov ntawm WILEY 09 thiab NIST 14 huab hwm coj spectraldatabase uas siv los txheeb xyuas thiab kawm txog cov tshuaj lom neeg tsis paub txog hauv ib qho extract (Wiley, 2006; Mikaia li al., 2014). Kev tshuaj xyuas tau ua tiav yam tsis zoo siv Thermo Scientific ™ Xcalibur ™ 2.2software, thiab tag nrho cov txiaj ntsig tau tshaj tawm hauv cov feem pua ​​​​ntawm cov txheeb ze (Abd El-Kareem li al., 2016).

Kev sim tsim

Txoj kev tshawb no tau pom zoo los ntawm Biomedical Research EthicsCommittee ntawm Kws Qhia Ntawv ntawm Tshuaj, King AbdulazizUniversity, Jeddah, KSA (saib naj npawb {{0}}). Hauv qhov kev tshawb fawb no, plaub caug tus txiv neej albino nas hnyav 150-200 g thiab hnub nyoog 2-3 lub hlis tau txais los ntawm King Fahd MedicalResearch Center (KFMRC). Ua ntej pib qhov kev sim, cov nas raug tso tseg kom haum rau qhov chaw kuaj mob rau 1 lub lis piam. Kaum nas tau raug xaiv los ua pawg tswj hwm uas tsis raug rau CUMS. Lwm peb caug nas tau raug rau cov txheej txheem ntawm CUMS rau 4 lub lis piam uas suav nrog ntau hom kev ntxhov siab ntawm cov sij hawm sib txawv nyob rau nruab hnub txhawm rau tiv thaiv kev ntxhov siab. Cov txheej txheem CUMS tau piav qhia tag nrho hauv cov haujlwm yav dhau los (Ayuob et al., 2016; Ali et al.,2017) thiab tau pom nyob rau hauv Table 1. Cov nas uas raug CUMSwere muab faib ua 3 pawg (n 10). Cov pab pawg tsis kho (CUMS) tau txais lub tsheb 0.03 feem pua ​​carboxymethyl cellulose (CMC-Na) los ntawm gavage rau 2 lub lis piam. FLU-kho pab pawg tau txais FLU (Dar Al Dawa Pharmaceuticals Co., Ltd., Amman, Jordan), ib qho tshuaj tiv thaiv kev nyuaj siab siv rau kev siv tshuaj kho mob, yaj hauv CMC-Na 0.03% ata koob ntawm 20 mg / kg los ntawm gastric gavage (Li li al. , 2014). CP-kho pab pawg tau txais CP extract yaj nyob rau hauv cov dej distilled ntawm ib koob ntawm 100 mg / kg los ntawm gavage rau 2 lub lis piam raws li Wang li al. (2012).

Kev ntsuas kev hloov tus cwj pwm

FST tau ua tiav tom qab 4 lub lis piam kom paub meej tias cov txiaj ntsig ntawm CUMS ntawm cov nas (Ali li al., 2017). Thaum lub sij hawm qhov kev sim no, txhua tus nas tau muab tso rau hauv lub khob iav cylindrical (qhov siab 20 cm, txoj kab uas hla 14 cm) nrog 15 cm dej ntawm 25 ± 2 degree. Tus nas tau yees duab rau 6 min siv tus cwj pwm software (Noldus InformationTechnology, EthoVision XT®), thiab tag nrho lub sij hawm siv mobile thaum 6 min raug ntsuas los ntawm tus kws kho qhov muag tsis pom kev rau pawg sim. Tag nrho lub sij hawm, nyob rau hauv vib nas this, siv los ntawm cov nas tsis muaj ceg tawv, tshwj tsis yog rau cov me me txav uas tsim nyog kom tus nas ntab, "lub sij hawm tsis muaj zog" thaum lub sij hawm 6 min tau txiav txim siab.

Cov txheej txheem biochemical

Nees nkaum plaub teev tom qab ua tiav qhov kev ntsuas tus cwj pwm, cov ntshav tau coj los ntawm cov kab mob intra-orbital ntawm nas tom qab raug tshuaj xyuas nrog 4 feem pua ​​​​ isoflurane (SEDICO PharmaceuticalsCompany, Cairo, Egypt) hauv 100 feem pua ​​​​ oxygen. Tom qab ntawd, ratswere euthanized los ntawm lub ncauj tsev menyuam dislocation. Cov ntshav kuaj tau centrifuged ntawm 3, 000 rpm rau 15 min ntawm 4 degree kom tau txais cov tshuaj serumand tau khaws cia ntawm −18 degree rau kev ntsuam xyuas biochemical.

Qib ntawm corticosterone (ALPCO Diagnostics, Orangeburg, NY, United States) tau soj ntsuam siv cov khoom siv enzyme-linkedimmunosorbent assay raws li cov chaw tsim khoom cov lus qhia. Raws li cov chaw tsim khoom cov lus qhia, TNF- thiab IL-6 (Quantakin R&D system, USA Kit) raug soj ntsuam los ntawm kev siv cov tshuaj tiv thaiv kab mob enzyme-linked immunosorbent. Theoptical ceev ntawm txhua tus qauv tau txiav txim siab nyob rau hauv duplicate nrog ib tug microplate ELISA nyeem ntawv teem rau 450 nm.

thiobarbituric acid reactive substances (TBARS) assay kit (Biodiagnostic; Egypt) tau siv los ntsuas qib ntawm malondialdehyde (MDA) spectrophotometrically ntawm 535 nm (Gamal li al., 2018). Qib ntawm superoxide dismutase (SOD) tau ntsuas los ntawm kev siv cov khoom siv SOD (Biodiagnostic; Egypt) (Packer, 2002). Kev soj ntsuam ntawm qib glutathione peroxidase (GPX) tau ua los ntawm kev siv cov khoom siv GPX (Randox Labs, Crumlin, United Kingdom). Txhawm rau kom muaj nuj nqis ntawm kev ua haujlwm ntawm catalase (CAT), ib qho kev ntsuas ntsuas tau tsim rau qhov ntsuas thiab cov qauv, siv cov khoom siv ntsuas (Biodiagnostic; Egypt). Cov txheej txheem tau piav qhia yav dhau los (Gamal li al., 2018).

suab puam cistanche extract cov txiaj ntsig: tiv thaiv kab mob

Quantitative Real-Time PCR

RNA rho tawm los ntawm cov ntaub so ntswg kuaj tau ua tiav siv covTriFast™ reagent (PeqLab, Lub teb chaws Yelemees, Cat No.: 30-2010), raws li tau piav nyob rau hauv cov chaw tsim tshuaj paus raws tu qauv. Qhov concentration ntawm purified RNA tau kwv yees los ntawm kev siv NanoDrop 2000cspectrophotometer (Thermo Scientific, United States). Cov rho tawm RNA los ntawm txhua tus qauv tau thim rov qab los ntawm SensiFAST™ cDNA synthesis cov khoom siv rau RT-qPCR(Bioline USA Inc., United States, Cat No.: BIO-65053), ua raws li cov chaw tsim khoom cov lus qhia. Cov synthesizedcDNA tau khaws cia ntawm -80 degree kom txog thaum siv qRT-PCR.

Cov tshuaj tiv thaiv qRT-PCR tau ua tiav siv SensiFAST™ SYBR Lo-ROX cov khoom siv (Bioline USA Inc., United States, Cat No.: BIO-94002) ntawm Applied Biosystems 7500real -time PCR nrhiav kom tau qhov system (Lub neej thev naus laus zis, Tebchaws Meskas).Gene-specific primers rau nas, siv hauv txoj kev tshawb no, tau tsim los ntawm Primer3 software (v.0.4.0), thiab lawv qhov tshwj xeeb tau kuaj xyuas siv NCBI/Primer-BLAST program. Cov primers tom qab ntawd tau yuav los ntawm Willowfort ™ (United Kingdom). The primerswere GAPDH (5′-TGCACCACCAACTGCTTAGC-3′, 5′-GGCATGACTGTGGTCATGAG-3}′), caspase-3 (forward 5- TGTATGCTACTCTACCGCACCCG-3, re 20}}GCGCAAAGTGACTGGATGAACC-3), HSP70 (5′-ACGAAGGGTCTCAAGGCAAG-3′, 5′-CTCTTTCAGCCAGCGGTTAG-3′).

Ki67 (forward 5-AGAAGAAGCCCACAGCACAGAGAA-3, rov qab 5-AGAGAGAGCCCACAGCAGAGAGAA 3). PCRmixture tau npaj raws li hauv qab no: 10 µl SensiFASTTM SYBRLo-ROX mix, 0.8 µl rau pem hauv ntej primer, 0.8 µl rov qab primer, 2 µltemplate cDNA, thiab 6.4 µl nuclease-dawb dej. Cov tshuaj tiv thaiv sib xyaw tau hloov mus rau lub thermal cycler uas tau ua dhau los kom tuav ntawm 95 degree rau 2 min, ua raws li 40 cyclesof 95 degree rau 15 s, thiab tom qab ntawd 60 degree rau 30 s. Ib qho kev tswj tsis zoo uas tsis muaj tus qauv tau khiav hauv txhua qhov kev sim.

Melting nkhaus tsom xam tau ua pov thawj qhov tshwj xeeb ntawm cov khoom PCR, thiab tus nqi Ct rau txhua qhov tshuaj tiv thaiv tau txais los ntawm cov phiaj xwm nthuav dav. Cov txheeb ze kom muaj nuj nqis rau txhua qhov geneexpression hauv cov ntaub so ntswg kuaj tau suav nrog qhov sib piv (ΔΔCt) txoj kev nrog GAPDH raws li kev tswj cov noob. Rau qhov hloov pauv tag nrho, nws tau suav thiab linearized los ntawm 2−ΔΔCt tus lej lej.

Cov txheej txheem Histological

Thaum kawg ntawm qhov kev sim thiab tom qab cov nas raug tshuaj xyuas, lub plab tau qhib thiab lub caj pas sab xis tau muab tshem tawm. Txhawm rau kom tau txais paraffin blocks, kho cov qog adrenal hauv 10 feem pua ​​​​ntxhiab tsw buffered formalinand cov txheej txheem ntxiv tau ua. Cov seem paraffin ntawm 4-μm thickness tau npaj thiab stained nrog hematoxylinand eosin (H&E). Ntxiv mus, lwm cov paraffin seem yog immunohistochemically stained siv thestreptavidin-biotin-peroxidase txheej txheem. Slides raug incubated hmo ntuj ntawm 4 degree , thiab ces lawv tau incubated nrog monoclonal anti-Ki67 (Dako Cytomation, Tebchaws Meskas, ntawm dilution 1: 1, 000) ​​rau kev ua qauv qhia ntawm cellproliferation. Tsis tas li ntawd, polyclonal anti-caspase{10}} antibody (Santa Cruz Biotechnology, United States, ntawm dilution 1: 1, 000) tau siv rau kev tshawb pom ntawmapoptosis. Ib qho polyclonalantibody tiv thaiv kev poob siab ntawm cov protein ntau-70 (HSP70) (Dako, Carpinteria, CA, United States, ntawm dilution 1: 1, 000) kuj tau siv. Kev siv biotinylated conjugated secondaryantibody los ntawm Dako staining system tau siv. Cov slides stained nrog cov tshuaj tiv thaiv theem nrab tsuas yog siv los tswj tsis zoo.

Lub nuclei tau counterstained nrog hematoxylin. Browncytoplasmic staining tau suav hais tias yog cov tshuaj tiv thaiv zoo. Cov seem raug tshuaj xyuas thiab yees duab siv anOlympus Microscope BX{{0}} (Olympus) txuas nrog lub koob yees duab digital thiab lub computer. Semiquantitative tsom xam ntawm antibodyimmunoreactivity yog ua los ntawm Pro Plus duab tsom xam software.Qhov feem pua ​​ntawm cov tshuaj tiv thaiv immunopositive tau soj ntsuam 30 teb ntawm × 400 magnification. Cov hlwb zoo tau suav nrog 1.0 mm2 ntawm thaj chaw, raws li tau piav qhia los ntawm Zhou li al. (2016). Yam tsawg kawg tsib daim teb los ntawm txhua qhov swb tau raug tshuaj xyuas, thiab qhov nruab nrab raug suav rau txhua tus tsiaj.

Rau kev tsom xam morphometric, plaub ntu tau tshuaj xyuas ineach tsiaj (zoom × 100). Lub thickness ntawm qhov sib txawv ntawm cov qog adrenal tau ntsuas hauv micrometers. Theadrenal cortex thickness yog tau los ntawm kev ntsuas qhov deb ntawm lub medulla thiab adrenal capsule nyob rau hauv ib txoj kab ncaj nraim, ib qho kev ntsuas yog coj nyob rau hauv txhua quadrant ntawm adrenalcortex.

Kev txheeb cais

Cov kev coj cwj pwm, biochemical, thiab immunohistochemical cov ntaub ntawv raug tshuaj xyuas los ntawm Cov Txheej Txheem Txheej Txheem rau Social Sciences (SPSS) version 16. Cov kev kawm sib txawv tau cuam tshuam los ntawm ob qho kev ywj pheej: kev ntxhov siab thiab kev kho mob. Yog li, kev tshuaj xyuas siv cov qauv sib xyaw ua ke ob txoj kev ANOVA raws li Bonferroni post hoc tau ua. Kev txheeb xyuas qhov tseem ceeb tau txiav txim siab ntawm p <>

suab puam cistanche cov txiaj ntsig: tiv thaiv kev laus

TSEEM CEEB

Cov txiaj ntsig ntawm tus cwj pwm

Lub sij hawm immobility thaum lub sij hawm FST tau ntau dua (p=0.03) nyob rau hauv CUMS pab pawg tshaj qhov kev tswj, whereas nws tseem ceeb heev (p=0.01, p=0.001) hauv FLU- thiab CP-kho pab pawg dua li hauv CUMS pawg (Daim duab 1A).

Cov txiaj ntsig ntawm CP Extract Analysis Siv GC-MS

Cov ntsiab lus tseem ceeb hauv CP feem ntau suav nrog oleic acid (kwv yees li 56 feem pua), palmitic acid (kwv yees li 8.9 feem pua), linolenic acid 3.5 feem pua, thiab linoleic acid 2.8 feem pua ​​​​ntawm ntau lwm cov tebchaw (Table 2; Daim duab 2). Ntawm cov tebchaw ntawm CP uas tau tshaj tawm tias muaj cov tshuaj tiv thaiv kab mob, muaj cov oleic acid, palmitic acid, linolenic acid, betulin, thiab linoleic acid, thaum cov neeg uas muaj cov kab mob.antioxidantcov nyhuv yog palmitic acid, 10-octadecenoic acid, thiab methyl ester.

Kev soj ntsuam biochemical

Corticosterone Level hauv Serum

Qib corticosterone tau nce ntxiv (p < {{0}}}.001) cov nas tsis kho tau raug CUMS piv rau cov nas tswj, thaum cov kws kho mob FLU thiab CP pom qhov txo qis (p <0.001) hauv cov ntshav corticosterone. qib piv rau cov nas uas tsis kho tau raug CUMS (Daim duab 1B).

TNF- thiab IL-6 Qib hauv Cov Ntshav

Thaum kawg ntawm qhov kev sim, kev soj ntsuam ntawm cov ntshav TNF- thiab IL-6 tau pom tias lawv tau nce siab heev (p < 0.001) hauv pawg CUMS, qhov uas lawv pom qhov txo qis (p< 0.001)="" in="" flu-="" and="" cp-treated="" groups="" compared="" with="" those="" in="" the="" untreated="" rats="" exposed="" to="" cums="" (figures="" 1c,="">

MDA, SOD, GPX, thiab CAT Levels hauv Serum

Nws tau pom tias qib ntawm cov ntshav MDA tau nce siab dua (p < {{0}}}.001)="" hauv="" pawg="" cums="" dua="" li="" cov="" nas="" tswj,="" thaum="" nyob="" hauv="" cp-kho="" pab="" pawg="" tau="" qis="" dua="" (p="" 0.003)="" tshaj="" qhov="" ntawd="" hauv="" cov="" nas="" uas="" tsis="" kho="" tau="" raug="" cums.="" fluadministration="" tsis="" tuaj="" yeem="" txo="" qis="" mda="" qib="" inrats="" raug="" rau="" cums="" (daim="" duab="">

Hauv qhov sib piv, qib ntawm SOD, GPX, thiab CAT hauv cov ntshav ntawm pawg CUMS tau qis dua (p < {0}}.001,="" p="">< 0.001,="" p="0" .002)="" dua="" li="" cov="" hauv="" cov="" ntshav="" ntawm="" pawg="" tswj="" hwm,="" thaum="" lawv="" cov="" qib="" tau="" pom="" qhov="" nce="" ntxiv="" (p="0.01," p="0.003," p="" 0.03)="" hauv="" pawg="" cp-kho="" tau="" piv="" rau="" cov="" hauv="" cums-exposedgroup.="" flu="" kev="" tswj="" hwm="" tsis="" tuaj="" yeem="" nce="" sod,="" gpx,="" thiab="" cat="" hauv="" cov="" nas="" uas="" raug="" rau="" cums="" (figures="">

Gene Expression of Ki67, Caspase-3, thiab HSP70

RT-PCR qhia qhov tsis tseem ceeb ntawm Ki67 noob qhia (p=0.14) hauv cov qog adrenal ntawm pab pawg CUMS piv rau pawg tswj hwm, thaum cov pab pawg FLU- thiab CP-kho tau pom muaj kev qhia siab (p {{6 }}. (p <0.001) hauv cov qog adrenal ntawm CUMSgroup piv rau pawg tswj hwm, thaum cov pab pawg FLU- thiab CP-kho tau pom qhov txo qis (p=0.03, p=0.04) piv rau cov pab pawg CUMS-exposed (Daim duab 3F).Thaum nws tuaj txog HSP70 gene qhia, nws tau sau ntau dua (p <0.001) qib hauv cov qog adrenal ntawm pawg CUMS dua li hauv pawg tswj hwm, thaum uas muaj FLU thiab CP-kho pab pawg tau pom qhov txo qis qis (p=0.07,p=0.08) piv rau cov pab pawg CUMS-exposed (Daim duab 3G).

Kev soj ntsuam Histopathological

Cov qog adrenal ntawm cov nas tswj tau muaj cov qauv tsis zoo nrog cov qauv tsim tshwj xeeb ntawm lub cortex nrog rau zona glomerulosa, zona fasciculata, thiab zona reticularis nrog rau medulla (Daim duab 4). Adrenal qog ntawm CUMS-tso nas pom pom ntau lub hlwb hauv ZG, ZF, thiab ZR nrog pom tseeb vacuolationand degenerated nuclei thiab qee zaum sib sib zog nqus nuclei nrog cov tuab tuab hauv cov tshuaj ntsiav thiab cov ntaub so ntswg sib txuas. Ntau lub hlwb ntawm ZR tshwm tsaus nti nrog cov xim brownlipofuscin. Ntawm qhov tod tes, cov qog adrenal ntawm CP-kho nas pom tau tias tsawg dua cov hlwb vacuolated thiab cov nuclei tob hauv ZG, thiab ZF thiab ZR qhia ob peb lub hlwb tsaus.

Kev ntsuas morphometric tau pom tias qhov tuab ntawm ZG thiab ZR pom qhov nce tsis tseem ceeb tom qab raug rau CUMS, thaum lawv tau nce ntau hauv FLU- (p 0.01,p 0.02) thiab CP- kho cov pab pawg (p=0.02, p=0.001), feem. Hais txog qhov tuab ntawm ZF, nws tau nce ntxiv (p 0.02) hauv CUMS-tshuaj pab pawg, thiab nws tau nce ntxiv, tab sis tsis tseem ceeb, hauv FLU- thiab CP kho cov pab pawg piv rau hauv pawg CUMS tsis kho.

Txhawm rau txiav txim siab seb cellular hyperplasia raug txwv rau ib cheeb tsam tshwj xeeb ntawm cov qog adrenal, cov tshuaj tiv thaiv immunohistochemical rau Ki67 (tus cim ntawm lub cell faib) tau siv. Tus naj npawb ntawm Ki67-cov hlwb zoo proliferating tau nce ntau (p < 0.001)="" hauv="" zf="" tom="" qab="" kis="" rau="" cums,="" thaum="" kho="" nrog="" flu="" thiab="" cpin="" tseem="" ceeb="" nce="" ntxiv="" piv="" rau="" cumsgroup="" tsis="" kho.="" txawm="" hais="" tias="" tus="" naj="" npawb="" ntawm="" cov="" hlwb="" proliferating="" tsis="" tseem="" ceeb="" hauv="" zg="" thiab="" zr="" thiab="" qis="" qis="" hauv="" medulla="" ntawm="" pawg="" cums="" piv="" rau="" pawg="" tswj="" hwm,="" kev="" kho="" mob="" nrog="" flu="" thiab="" cp="" tau="" nce="" ntau="" ntawm="" cov="" hlwb="" proliferating="" hauv="" zg="" (p="0.001)." ,="" p="0.003)," zr="" (p="">< 0.001)="" thiab="" medulla="" (p="0.004," p="0.003))" piv="" rau="" pawg="" cums="" tsis="" kho="" (daim="" duab="" 4,="">

Immunohistochemical staining siv caspase-3 tau ua los ntsuasapoptosis(Daim duab 6). Nws tau pom tias thaj tsam feem pua ​​​​ntawm caspase-3 qhia tau nce ntau ntxiv (p < 0.001)="" hauv="" txhua="" thaj="" chaw="" ntawm="" lub="" caj="" pas="" tom="" qab="" raug="" cums="" rau="" 4="" lub="" lis="" piam="" hauv="" kev="" sib="" piv="" ntawm="" pawg="" tswj="" hwm.="" pab="" pawg="" kho="" mob="" cp="" tau="" pom="" qhov="" txo="" qis="" hauv="" caspase-3qhia="" hauv="" zg="" (p="">< 0.001),="" zf="" (p="">< 0.001),="" zr="">< 0.001,="" p=""
="" 0.005),="" and="" medulla="" (p="0.004," p="0.001)," in="" comparison="" to="" that="" of="" the="" cums="" group="" (figures="">

Qhov feem pua ​​​​ntawm HSP70 qhia tau nce ntxiv (p < 0.001) hauv txhua thaj chaw ntawm lub cortex nrog rau hauv medulla ntawm cov qog adrenal ntawm pawg CUMS piv rau pawg tswj hwm, qhov uas nws tau txo qis hauv qhov tseem ceeb. tag nrho cov zonesand medulla ntawm FLU- thiab CP-kho pab pawg piv rau cov pab pawg CUMS (Figures 5).

Qhuav Cistanche tubulosa slices

Kev sib tham

Lub neej niaj hnub no pom tseeb tias muaj kev ntxhov siab uas yuav tshwm sim los ntawm cov teeb meem niaj hnub, kev saib xyuas kev noj qab haus huv, thiab cov teeb meem ntsig txog haujlwm. Cov kev ntxhov siab no yuav ua rau muaj kev ntxhov siab ntsig txog kev ntxhov siab (Clayton andMccance, 2014). Nyob rau hauv txoj kev tshawb no, ethanolic extract ntawm cov nqaij thiab tev ntawm Cucurbita pepo L. tau siv los tshawb xyuas nws lub peev xwm los txo tus cwj pwm kev nyuaj siab CUMS thiab nws cuam tshuam rau lub qog adrenal. Qhov no tau ua pov thawj los ntawm kev nce qib hauv cov ntshav corticosterone thiab tau txheeb xyuas los ntawm lub sijhawm ntev immobility thaum lub sijhawm FST. Cov kev tshawb pom no tau ua raws li cov lus qhia yav dhau los (Tang li al., 2015).

Ob leeg TNF- thiab IL-6 tau tshaj tawm tias muaj kev koom tes hauv kev tsim kev nyuaj siab (Taraz li al., 2015; Pedraz-Petrozziet al., 2020). Nws tau tshaj tawm tias cov nas tsis muaj peev xwm ntawm IL-6 lossis TNF- receptors tiv taus cov cwj pwm kev nyuaj siab (Viana li al., 2010).Yog li ntawd, lawv tau soj ntsuam hauv txoj kev tshawb no, thiab nws tau pom tias ob qho tib si TNF- thiab IL{ {9}} tau nce ntau ntxiv hauv cov nas ntawm cov nas kis rau CUMS rau 4 lub lis piam, qhia tias lawv muaj kev nyuaj siab. Qhov kev tshawb pom no tau txhawb nqa los ntawm Numakawa et al.(2014) nyob rau hauv nas nrog kev poob siab ntawm tus cwj pwm thiab los ntawm Taraz li al. (2015) hauv cov neeg mob uas muaj kev nyuaj siab.

Nyob rau hauv txoj kev tshawb no, kev tswj hwm ntawm taub dag extract yog txuam nrog amelioration ntawm tus cwj pwm kev nyuaj siab, tshwm sim los ntawm qhov txo qis ntawm lub sij hawm immobility ntawm FST thiab tau lees paub los ntawm qhov txo qis incorticosterone thiab inflammatory cytokines TNF- thiab IL-6 hauv cov ntshav. Cov kev tshawb pom no tau txhawb nqa los ntawm Kim Nr et al. (2016) uas tau tshaj tawm tias SSP rau 28 hnub txo qis qib ntawm cov kab mob cytokines hauv cov nas muaj kev nyuaj siab. Anti-inflammatory kev ua ntawmtaub dagTej zaum yuav raug ntaus nqi rau cov khoom sib txuas ntawm taub dag xws li oleic thiab palmicacids thiab estradiol.

Kev txo qis ntawm oleic acid, palmitic acid, thiab linoleic acid tau piav qhia hauv kev nyuaj siab (Conklin li al., 2010; Martín li al., 2010). Nws tau tshaj tawm tias oleic acid-mediated neuroprotectionis txuas rau nws cov tshuaj tiv thaiv kab mob sib kis los ntawm peroxisomeproliferator-activated receptor gamma (PPAR-) ua kom (Song et al., 2019). Ntxiv rau qhov ntawd, palmitic acid tau tshaj tawm tias inhibit phospholipase A(2) thiab yog li ntawd. suav hais tias yog ananti-inflammatory compound (Aparna li al., 2012). Estradiol yog ib qho ntawm cov khoom sib txuas ntawm taub dag pom nyob rau hauv txoj kev tshawb no.Estradiol tau tshaj tawm tias yuav ua rau muaj kev tiv thaiv kab mob thiab tom qab ntawd txo qis tus cwj pwm kev nyuaj siab (Xu li al., 2015).Lwm yam uas estradiol tuaj yeem ua rau cov nyhuv anantidepressant yog kev tswj hwm ntawm cov tshuaj. Kev qhia ntawm lub hlwb-derived neurotrophic factor (BDNF) thiab ERK phosphorylation los ntawm kev ua kom ER- hauv lub hlwb (Yang li al., 2010).

Hauv txoj kev tshawb no, qib ntshav ntawm MDA tau nce, thaum SOD, GPX, thiab CAT qib tau txo qis hauv CUMS-exposedrats. Cov kev hloov pauv zoo sib xws tau sau tseg tom qab raug rau CUMS hauv kev tshawb fawb yav dhau los, thiab lawv tau ntxiv tias cov kev hloov pauv biochemical no ua rau muaj kev ntxhov siab oxidative thiab kev puas tsuaj ntawm HPA (Nabavi li al., 2015). Liu T. et al. (2015) kuj tau tshaj tawm tias txoj kev loj hlob ntawm kev nyuaj siab yuav raug ntaus nqi rau cov ntsiab lus ntawm cov tshuaj antioxidants hauv lub cev. Antioxidant muaj nuj nqitaub dagtxiv hmab txiv ntoo extract tau nquag qhia (Bahramsoltani li al., 2017). Tsis tas li ntawd, cov nqaij thiab tev ntawm Cucurbita pepo L. tau qhia txog kev ua haujlwm antioxidant ntau dua li lwm qhov, piv txwv li, noob (Oyeleke li al., 2019). Pumpkin extract kev tswj hwm, ntau cov kev tshawb fawb yav dhau los, nce qib ntawm SODand GPX thiab txo MDA hauv cov ntshav ntawm cov nas (Guo-Huaet al., 2000; Dang, 2004), thiab cov nyhuv no kuj tau sau tseg hauv txoj kev tshawb no. Yog li ntawd, lubantioxidantKev ua ntawm taub dag tuaj yeem ua rau tom qab nws cov nyhuv antidepressant pom tseeb hauv txoj kev tshawb no. Qhov no yog ib txoj kab nrog kev soj ntsuam ntawm Kim Nr et al. (2016). Nws tau tshaj tawm tias cov khoom ntuj tsim nrog cov tshuaj tiv thaiv,antioxidant, thiab los tiv thaiv kev qaug zog kuj muaj cov nyhuv anantidepressant (Jeong li al., 2015). Tag nrho cov haujlwm yav dhau los no tau ua pov thawj hauvtaub dag.

Cistanche muaj cov nyhuv anti-aging

Kev nce cytoplasmic vacuolation ntawm adrenal cortical hlwb, pom nyob rau hauv CUMS pawg hauv txoj kev tshawb no, tej zaum yuav raug ntaus nqi rau qhov kev thov nce rau lipids, uas tsim lub hauv paus pob zeb forcortisol synthesis. Cigankova et al. (2005) pom tias kev sim siab ntev ntev ua rau muaj kev sib koom ua ke ntawm ntau lub cytoplasmic lipid droplets ntawm cortical hlwb. Cov lipiddroplets no muaj cov roj cholesterol, uas yog lub hauv paus tseem ceeb hauv kev tsim cov tshuaj hormones steroid. Txawm li cas los xij, Koldysheva thiab Lushnikova (2008) tau tshaj tawm tias kev ntxhov siab ua rau muaj qhov txo qis hauv lipid droplets hauv hlwb ntawm adrenal cortex, tshwj xeeb tshaj yogZF (Koldysheva thiab Lushnikova, 2008). Nws tau piav qhia tias thaum raug rau lub cev kev ntxhov siab, qhov mob stimuli raug xa mus rau hypothalamus, ua rau CRH zais cia rau hauv lub cev lub cev, uas ua rau ACTH secretion thiab txhawb nqa nws cov receptors hauv ZF thiab ZR kom nce cortisol secretion (Patti li al., 2018). Cov thickened adrenalcapsule thiab trabeculae pom nyob rau hauv CUMS-exposed nas tau tshaj tawm yav dhau los (El-Desouki li al., 2011; Altayeb thiab Salem, 2017) vim yog kev nce collagen synthesis los ntawm fibroblasts oneexposure rau immobilization kev nyuaj siab.

Ntau lub hlwb nyob rau hauv cov qog adrenal ntawm CUMS-tso tawm nas tau pom cov kab mob sib sib zog nqus, qhia tias lawv muaj kev tsis txaus ntseeg uas tau lees paub los ntawm caspase-3 immunohistochemicalstaining. Ib qho kev soj ntsuam zoo sib xws tau tshaj tawm los ntawm Altayeb thiab Salem, (2017), thaum lawv tab tom kawm txog cov txiaj ntsig ntawm immobilizationstress ntawm cov qog adrenal ntawm nas. Liu Q. et al. (2015) tau tshaj tawm tias kev ntxhov siab oxidative ntau dhau, tshwm sim tom qab raug rau CUMS hauv txoj kev tshawb no, hloov kho cov theem ntawm "apoptosis-relatedgenes" thiab induces cell apoptosis thiab degeneration los ntawm kev taw qhia txoj hauv kev ntawm Bcl-2, Bax, thiab caspase-3. Cov txheej txheem no tau lees paub hauv qhov kev tshawb fawb no los ntawm RT-PCR uas qhia txog kev txhim kho tseem ceeb ntawm caspase -3 gene Kev tshuaj xyuas cov ntaub ntawv, tsis muaj qhov cuam tshuam ncaj qha ntawm Cucurbita pepo ntawm caspase-3-mediated apoptosis hauv cov nqaij sib txawv. Nws zoo li qhov txo qis hauv caspase-3-cov apoptoticcells pom zoo nyob rau hauv cov qog adrenal tau raug ntaus nqi rau covantioxidantCov txiaj ntsig ntawm Cucurbita pepo tau sau tseg hauv txoj kev tshawb no.Qhov no tau txhawb nqa los ntawm ntau cov kev tshawb fawb yav dhau los ua rau lwm cov nroj tsuag thiab tshuaj ntsuab nrog cov haujlwm tseem ceeb antioxidant (Banagozar Mohammadi li al., 2019; Ghazizadeh li al., 2020).

Txoj kev tshawb no qhia tau hais tias raug rau CUMS nce Ki67- cov hlwb zoo nyob rau hauv lub adrenal cortex uas tau tshwm sim nyob rau hauv lub ZF, qhia tau hais tias ib tug ntau ntawm cov proliferating hlwb, thiab yog li ntawd, lub thickness ntawm cov txheej no yog ho nce. Lwm qhov tes, tus lej ntawm Ki67-cov cell proliferating zoo txo ​​qis hauv cov medulla teb rau CUMS.Cov kev soj ntsuam no tau nyob rau hauv ib txoj kab nrog cov Ulrich-Lai li al.(2006) uas tau tshaj tawm txog kev nce hauv Ki{{ 4}}zoo cell nuclei tsuas yog nyob rau sab nrauv ZF tom qab raug cov kev ntxhov siab tsis ntev los no, thaum themedulla pom cov cellular hypertrophy thiab tsis yog hyperplasia.Ntxiv mus, Laborie li al., (2003) tau tshaj tawm txog kev nce ntxiv hauv medullary muaj nuj nqi tom qab raug kev nyuaj siab ntev, qhia tias Kev ntxhov siab ntev yuav ua rau medullary hypertrophy.Nyob rau hauv txoj kev tshawb no, kev tswj hwm ntawm covtaub dagrho tawm cov cell proliferation ntau ntxiv, pom tseeb los ntawm kev tswj hwm ntawm cov noob caj noob ces Ki67 thiab kev tiv thaiv kab mob hauv yuav luag txhua thaj chaw thiab themedulla ntawm cov qog adrenal piv rau CUMS. Qhov no tau piav qhia txog qhov nce ntxiv ntawm cov qog qog nqaij hlav hauv qhov kev tshawb fawb no. Nws kuj tseem tuaj yeem them nyiaj rau cov nyhuv apoptotic tshwm sim los ntawm kev raug rau CUMS. Qhov kev tshawb pom no tau txhawb nqa los ntawm Kim Hy thiab al. (2016) whoreported ib tug tseem ceeb upregulation ntawm lub mRNA qhia ntawmKi67 thiab proliferation ntawm splenocytes cais los ntawm tus po ntawm BALB/c nas kho nrog SSP, streamed, thiab Cucurbita moschataDuch thiab nws cov ntsiab lus tseem ceeb, -carotene.

Hauv txoj kev tshawb fawb tam sim no, raug rau CUMS tau nthuav tawm cov noob caj noob ces thiab kev tiv thaiv kab mob ntawm HSP70 hauv txhua qhov chaw ntawm cov qog adrenal. Nrog rau qhov ntawd, Zheng et al. (2008) kuj tau ua pov thawj tias augmented HSP70expression tom qab raug rau cov kev nyuaj siab modulatesinflammatory cov lus teb los ntawm inhibiting activation ntawm lub inflammatory transcription factor, lub nuclear factor-kappa B (NF-kappaB). Tsis tas li ntawd, HSP70 tuaj yeem cuam tshuam ncaj qha rau apoptosis thiab necrosis (Yenari li al., 2005). Tsis ntev los no, Liet al. (2019) tau tshaj tawm txog kev tswj hwm ntawm HSP70 kev qhia hauv cov qog adrenal cov ntaub so ntswg tom qab raug tshav kub ntxhov siab, uas qhia txog lub luag haujlwm ntawm HSP70 hauv cov qog adrenal raug mob thiab hais txog nws qhov cuam tshuam rau cov lus teb. Kev nrawm nrawm ntawm HSP70 hauv kev teb rau kev ntxhov siab yog suav tias yog qhov tseem ceeb rau cov txheej txheem tiv thaiv cellular.

Hauv peb txoj kev tshawb fawb, kho CUMS-exposed nas nrogtaub dagtau koom nrog qhov txo qis hauv HSP70 qhov kev qhia uas tau cuam tshuam nrog txo qis apoptosis thiab inflammatory teb. Qhov no tej zaum yuav taw tes rau cov txiaj ntsig zoo ntawm cov qib siab ntawm HSP70 cuam tshuam los ntawm FLU thiab CP vim qhov ua rau txo qis ntawm caspase-3-kev kho mobapoptosisandrelease ntawm inflammatory cytokines. Txawm hais tias cov txiaj ntsig ntawm taub dag rau cov tsev neeg cov khoom noj muaj cua sov tsis tau piav qhia dhau los, oleic acid, uas sawv cev rau cov khoom tseem ceeb ntawmtaub dagsiv nyob rau hauv txoj kev tshawb no, tau piav qhia txog downregulatethe qhia ntawm HSP60 nyob rau hauv tib neeg T lymphocyte cell kab (Martins De Lima li al., 2004), uas yog txhawb rau peb kev tshawb nrhiav. Lub tswv yim txheej txheem ntawm kev txiav txim ntawm Cucurbita pepo asan antidepressant-zoo li tshuaj yog sau tseg nyob rau hauv daim duab 7. Ntawm cov kev txwv ntawm txoj kev tshawb no yog qhov tsis muaj peev xwm mus tshawb xyuas qhov tob tob ntawm cov tshuaj tiv thaiv kev nyuaj siab ntawm CP, thiab yog li ntawd, kev kawm ntxiv tau txhawb kom ua qhov ntawd. .

Hauv kev xaus, qhov kev tshawb fawb no muab pov thawj kev tshawb fawb txog kev ua tau zoo ntawm Cucurbita pepo L. extract los txo CUMS-vim kev coj cwj pwm thiab kev hloov pauv biochemical nrog rau kev cuam tshuam ntawm cov qog adrenal. Cov teebmeem no tshwm sim los ntawm kev txo qis ntawmapoptosisthiab HSP70 qhia thiab zoo li yuav tsum tau kho kom haum los ntawmantioxidantthiab anti-inflammatory nyhuv ntawm Cucurbita pepoL. rho tawm. Txawm hais tias txoj kev tshawb no thiab qee qhov kev tshawb fawb tsis ntev los no tau sau tseg cov tshuaj tiv thaiv zoo li Cucurbita pepo thiab tshawb nrhiav nws cov txheej txheem, cov kev tshawb fawb ntxiv nrog rau cov kev tshawb fawb soj ntsuam yuav tsum tau ua kom pom tseeb cov nyhuv no rau tib neeg.

Cistanche Granules