Ib Txoj Kev Ua Mullein Los Ntawm Biocatalytic Hloov Hloov Ntawm Cistanche tshiab

Abstract

Tam sim no invention qhia ib txoj kev los tsim mullein los ntawm lubKev hloov pauv biocatalyticntawmtshiab Cistanche. Cov txheej txheem ntawm kev tsim khoom tam sim no siv Cistanches tshiab raws li cov khoom siv raw, los ntawm cov txheej txheem ntawm cell crushing, slurry extraction thiab high-temperature enzyme inactivation txheej txheem, lub inactivated Cistanches cell slurry yog siv raws li catalytic cov tshuaj tiv thaiv substrate, thiab -glucosidase muaj enzyme. immobilized enzyme yog siv los ua biocatalyst los ua cov tshuaj tiv thaiv tsis tu ncua lossis txuas ntxiv catalytic rau catalyze hydrolysis ntawm pineal glucoside nyob rau hauv lub substrate kom nws ces, los ntawm kev sib cais thiab purification txheej txheem thiab concentration thiab qhuav txheej txheem, lub mullein monomer compound yog sau; los ntawm kev kho cov xwm txheej ntawm kev sib cais thiab kev ua kom huv, qhov purity feem pua ​​​​ntawm cov qoob loo mullein monomer compound tuaj yeem ncav cuag 50 feem pua ​​-90 feem pua. Lub monomeric compound ntawm mullein muaj cov yam ntxwv ntawm ib tug me me molecule thiab tsis tshua muaj polarity, uas muaj ib tug zoo haum tus nqi thiab tshwj xeeb pharmacological kev ua si nyob rau hauv cov tshuaj nqus.

Nyem qhov no kom tau txais 8-16 feem pua ​​Acteoside Cistanche Extract

Kev piav qhia

Ib txoj hauv kev tsim cov mullein los ntawm biocatalytic hloov pauv ntawm Cistanches tshiab

Technical Field

Tam sim no invention muaj feem xyuam rau ib txoj kev tsim mullein monomeric tebchaw los ntawm biocatalytic transformation ntawm tshiab Cistanches.

Keeb kwm Technology

Herba Cistanches, tshuaj ntsuab uas muaj kab mob perennial, yog cov tshuaj suav tshuaj tonic nto moo nrog cov txiaj ntsig ntawm cov txiaj ntsig zoo, tonifying lub raum, thiab ncua kev laus. Kev tshawb fawb niaj hnub no qhia tau hais tias cov khoom xyaw tseem ceeb ntawm Herba Cistanches yog phenylethanol glycosides, benzyl cawv glycosides, cyclic enol ether terpene glycosides, lignan glycosides, oligosaccharide derivatives, D-mannitol thiab betaine. Phenylethanoid Glycosides (Ph Gs) suav nrog cov tshuaj nquag xws liEchinacosidethiabActeoside, thiab nws tau pom los ntawm txoj hnyuv kab mob metabolism uas cov txheej txheem txav ntawm Phenylethanoid Glycosides hauv plab hnyuv yuav tsum tau catalyzed los ntawm microbial enzymes nyob rau hauv txoj hnyuv loj kom hloov mus rau hauv.Acteoside. Lub metabolic channel ntawm pineal glycoside nyob rau hauv lub plab zom mov yog qhov tseem ceeb ua rau muaj qhov sib txawv ntawm bioavailability thiab kev ua tau zoo tom qab kev tswj qhov ncauj. Lub monomeric compound ntawm mullein muaj cov yam ntxwv ntawm cov molecule me me thiab polarity tsis tshua muaj, ua kom pom tus nqi nqus tau zoo thiab tshwj xeeb cov tshuaj pharmacological hauv kev nqus tshuaj.

Cov ntsiab lus ntawm Invention

Lub hom phiaj ntawm qhov kev tsim kho tam sim no yog los muab ib txoj hauv kev rau kev tsim cov mullein glycoside monomeric tebchaw los ntawm Cistanches tshiab raws li cov khoom siv raw los ntawm cov txheej txheem kev kho mob thiab kev hloov pauv catalytic hauv kev lag luam immobilized enzyme reactor.

Cov txheej txheem ntawm kev tsim khoom tam sim no yog siv Cistanche tshiab ua cov khoom siv raw, kho nws los ntawm kev sib tsoo ntawm tes, cov txheej txheem slurry extraction thiab cov txheej txheem kub-kub enzyme inactivation, siv cov inactivated Cistanche cell slurry li catalytic cov tshuaj tiv thaiv substrate, immobilize enzyme los ntawm -glucosidase. muaj enzyme npaj raws li ib tug biocatalyst, catalyze lub hydrolysis ntawm pineal glucoside nyob rau hauv lub substrate kom tawg qhov kawg ntawm glucosyl ligand thiab hloov mus rau heev nquag marocain monomer ces, los ntawm kev sib cais thiab purification txheej txheem, thiab cov concentration thiab ziab txheej txheem, mullein monomer. compound tau sau; cov txheej txheem catalytic kub yog 38 degree -60 degree, pH yog 4.0-5.8, lub sij hawm yog 4h-8h, thiab -glucosidase kev ua hauv cov tshuaj tiv thaiv cov khoom yog 2U / g -1000U/g; los ntawm kev kho cov txheej txheem kev sib cais thiab kev ua kom huv si, cov qoob loo mullein monomer compound Cov feem pua ​​​​purity ntawm cov qoob loo mullein monomers tuaj yeem ncav cuag 50 feem pua ​​-90 feem pua.

Cov kauj ruam tshwj xeeb ntawm txoj kev ntawm qhov kev tsim kho tam sim no suav nrog:

1. xaiv cov txiv hmab txiv ntoo tshiab ntawm cistanche tubulosa, muab tso rau hauv lub tank ntxhua thiab yaug nrog dej txias lossis dej ntshiab; khaws lawv, muab tso rau hauv ib txwm muaj crushing thiab sib tsoo cov cuab yeej los tsoo thiab zom cov nqaij stems ntawm cistanche tubulosa, ntxiv cov dej ntshiab los yog ua ntej chilled muaj dej ntshiab los ntxuav cov hlwb tawg kom ua cistanche tubulosa crushed cell pulp; tom qab ntawd siv lub tshuab lim dej ib txwm siv los nias thiab lim los yog muaj centrifuge cais lawv thiab tau txais cistanche tubulosa cell pulp; Tom qab ntawd Cov lim dej tuaj yeem muab ntxiv rau cov dej ntshiab los yog cov dej ua ntej txias dej ntshiab ib zaug los yog ob peb zaug (feem ntau 1 mus rau 3 zaug) thiab ntxiv hauv av rau hauv cov kua txiv hmab txiv ntoo los ntawm cov niaj hnub kev lag luam crushing thiab sib tsoo tshuab, thiab cov hlwb crushed tuaj yeem ua tau. muab rho tawm los ntawm ib txwm muaj ultrasonic extraction ntaus ntawv tib lub sij hawm, thiab ces sib cais los ntawm cov lim xovxwm los yog industrial centrifuge kom tau cistanche tubulosa cell pulp. txheej txheem cistanche tubulosa crushed cell pulp thiab cell pulp yog tswj ntawm qhov kub qis dua 20 degree (feem ntau yog 4-20 degree );

2, cistanche cell pulp tau los ntawm kauj ruam 1 yog inactivated ntawm kub kub los ntawm ib txwm muaj cua sov ntaus ntawv, los yog industrial ultra-siab kub instantaneous inactivation yog nqa tawm; Lub inactivated cistanche cell pulp yog siv los ua catalytic cov tshuaj tiv thaiv substrate, thiab -glucosidase industrial enzyme npaj immobilized enzyme tau los ntawm ib txwm immobilized enzyme industrial zus tau tej cov adsorption txoj kev yog siv raws li lub biocatalyst, thiab cov tshuaj tiv thaiv yog nqa tawm los ntawm ib txwm muaj immobilized. enzyme reactor Nruam los yog tsis tu ncua catalytic cov tshuaj tiv thaiv, catalytic hydrolysis kom tshem tawm cov piam thaj hauv qhov kawg ntawm glucosyl ligand ntawm substrate pineal glucoside, kev hloov pauv hloov mus rau mullein monomer compound; catalytic cov txheej txheem kub yog 38 degree ~ 6 0 degree, qhov kub zoo tshaj plaws yog 42 degree ~ 48 degree; pH yog 4.0 ~ 5.8, pH zoo tshaj plaws yog 4.5 ~ 5.3; Lub sijhawm yog 4h ~ 8h, lub sijhawm zoo tshaj plaws yog 5h ~ 6h. Qhov kev ua -glucosidase ntawm cov khoom siv tshuaj tiv thaiv hauv cov txheej txheem catalytic cov tshuaj tiv thaiv yog 2U / g-1000U / g, thiab cov enzyme zoo tshaj plaws yog 25U / g-150U / g; Kev hloov pauv tus nqi ntawm pineal glucoside rau hauv myricetin monomer compound hauv cov khoom siv tshuaj tiv thaiv hauv cov txheej txheem catalytic cov tshuaj tiv thaiv tau ntsuas yog 78 feem pua ​​-95 feem pua

3, cov tshuaj tiv thaiv tom qab cov tshuaj tiv thaiv ntawm cov kauj ruam 2, siv cov niaj hnub muaj ultrafiltration membrane sib cais, xaiv sieving thiab tuav ntawm polysaccharides, proteins thiab lwm yam loj molecule impurities, raws li zoo raws li particulate thiab sub-particulate impurities; thiab tom qab ntawd siv cov kev lag luam niaj hnub nanofiltration membrane sib cais, xaiv sieving thiab tuav cov amino acids, D-mannitol, betaine thiab lwm yam me me molecule impurities; kuj tseem tuaj yeem siv dua siv cov txheej txheem kev lag luam macroporous resin adsorption kev sib cais, lossis / thiab siv cov txheej txheem chromatographic kem sib cais thiab kev ua kom huv, lossis / thiab cov txheej txheem ib txwm ua rau lub txaj chromatography cais cov txheej txheem los yog cov txheej txheem ntau yam chromatography sib cais rau ntxiv kev sib cais thiab purification; los ntawm kev kho cov kev sib cais saum toj no thiab cov txheej txheem purification, sib txawv purity rho tawm ntawm mullein monomer tebchaw tuaj yeem tau txais; thiab

4, cov extract ntawm mullein monomer compound tau nyob rau hauv cov kauj ruam 3, los ntawm ib txwm muaj lub tshuab nqus tsev concentration txheej txheem los yog muaj nyias zaj duab xis evaporation concentration txheej txheem, kom tau ib tug solids ntsiab lus ntawm 30% mus rau 50 feem pua ​​ntawm cov concentrate; thiab tom qab ntawd los ntawm cov txheej txheem tshuaj tsuag kom qhuav, lossis lwm yam kev lag luam kom qhuav, kom tau cov hmoov los yog qhuav teeb meem ntawm mullein monomer compound. Qhov feem pua ​​​​purity cov ntsiab lus ntawm cov qoob loo mullein monomer tebchaw tuaj yeem ncav cuag 50 feem pua ​​​​rau 90 feem pua.

Cov tshiabCistanche raw khoomKev koom tes hauv cov txheej txheem ntawm qhov kev tsim tawm tam sim no tuaj yeem raug khaws cia tshiab (tsis yog tub yees lossis khov thiab khaws cia) nqaij tawv ntawm Cistanche, lossis cov nqaij tawv tshiab ntawm Cistanche khaws cia hauv cov tub yees, lossis cov nqaij tshiab ntawm Cistanchefrozen nyob rau hauv lub freezers; los yog hlais los yog shredded fleshy stems ntawm tshiab Cistanche raws li hais saum toj no.

Lub Cistanche tau piav qhia nyob rau hauv tam sim no invention is cistanche tubulosa YC Malos yogCistanche tubulosa (Schrenk)Wight ntawm tsev neeg Lietangidae, uas yog suav nrog hauv 2005 tsab ntawm Pharmacopoeia ntawm Cov Neeg Sawv Cev ntawm Tuam Tshoj.

Cov txheej txheem ntawm kev tsim muaj kev siv cov khoom txias cia khaws cia ntawm qhov chaw khaws cia kub yog feem ntau 1 degree ~ 13 degree, qhov zoo tshaj plaws txias cia khaws cia ntawm lub reservoir kub yog 4 degree ~ 10 degree; Kev siv lub freezer tub yees ntawm lub reservoir kub yog feem ntau -52 degree ~ -10 degree, qhov zoo tshaj plaws freezer tub yees ntawm lub reservoir kub yog -36 degree ~ -18 degree ; Kev siv cov dej txias tso rau hauv lub tank dej kub yog 1 degree ~ 20 degree, qhov zoo tshaj plaws txias dej kub yog 4 degree ~ 10 degree Tus nqi ntawm cov dej ntshiab los yog muaj dej ntshiab ntxiv los ntawm pre-txias txhua zaus yog 100 feem pua. mus rau 400 feem pua ​​(qhov hnyav piv) ntawm cov crushed hlwb los yog lim residue ntawm cistanche tubulosa, thiab qhov zoo tshaj ntxiv yog 200 feem pua ​​​​rau 300 feem pua ​​(qhov hnyav piv); Cov dej kub ntawm cov dej ntshiab huv los ntawm kev ua ntej txias yog 1 degree txog 20 degree, thiab cov dej kub zoo tshaj plaws yog 4 degree txog 10 degree.

Cov txheej txheem ntawm kev tsim muaj kev lag luam tsim cov txheej txheem adsorption kom tau txais immobilized enzyme, feem ntau -glucosidase industrial enzyme npaj enzyme tov thiab adsorbent tiv tauj, thiab tom qab ntawd ntxuav kom tshem tawm cov tshuaj tsis adsorbed enzyme tuaj yeem ua immobilized enzyme; nrog rau txoj kev siv lub cev adsorption, ion adsorption method; adsorbent suav nrog microporous iav, hydroxyapatite, cellophane, macroporous hluavtaws cob, tshwj xeeb ceramics, DEAE - cellulose, DEAE-dextran gel, Amberlite IRA-93, IRA-410, IRA-900, Dowex{{ 7}}, Amberlite CG-50, IRC-50, IR-120, uas tuaj yeem xaiv los ntawm cov khoom lag luam txawv teb chaws thiab txawv teb chaws.

Cov txheej txheem ntawm kev tsim khoom tam sim no suav nrog -glucosidase industrial enzyme npaj, suav nrog -glucosidase thiab cellulase enriched nrog -glucosidase, cog extract compound enzyme, cog hydrolysis compound enzyme.

Cov txheej txheem ntawm kev tsim khoom tam sim no suav nrog kev kub siab ntawm cov enzyme feem ntau ntawm 75 degree txog 100 degree, thiab lub sijhawm feem ntau yog los ntawm 3min mus rau 10min; Qhov kub ntawm ultra-siab kub instantaneous inactivation ntawm enzyme feem ntau yog los ntawm 135 degree mus rau 141 degree.

Kev lag luam immobilized enzyme reactor koom nrog hauv cov txheej txheem ntawm qhov kev tsim tawm tam sim no tuaj yeem yog ib txwm muaj kev lag luam stirred tank reactor, immobilized txaj reactor lossis fluidized txaj reactor.

Cov txheeb ze molecular loj ntawm ultrafiltration membrane koom nrog hauv txoj kev tsim khoom yog 1000 txog 4500, thiab qhov zoo tshaj plaws txheeb ze molecular loj yog 1500 txog 3500; qhov txheeb ze molecular loj ntawm nanofiltration membrane yog 200 mus rau 300, thiab qhov zoo tshaj plaws txheeb ze molecular loj yog 250 mus rau 280; lub ultrafiltration membrane yog xaiv los ntawm series ntawm membrane qauv ntawm CA los yog CTA, PAN, PS, PSA, PES, PVDF, PEK, SPS Rau nanofiltration membranes, 4040-UHT-ESNA los yog 8540-UHY-ESNA , ESNA-FREE650, ESNA-FREE1700, NTR7450HG, NTR729HG, NF-CA daim nyias nyias, NF-CA dov cov ntsiab lus thiab hollow fiber ntau yog siv; rau macroporous adsorption resins, XAD, Diaion, SP HPD500, HPD600, HPD8, H107, JD-KW, LD601, LD605, ME-1, ME-2, ME-3, NKA{{ 30}}, NKA-9, RA, S8, SIP, WLD thiab X-5 series qauv; simulated txav txaj chromatography cais qhov system thiab multifunctional chromatography sib cais system, koj tuaj yeem xaiv MB, MD, ME, MF npaj chromatographic cais chromatograph, SMBC sim hom, SMBC pilot type, XZ12E-4L hom, XZ20Z-2 L hom thiab SMBC industrial nruam npaj chromatographic hom chromatographic sib cais system. Lub ultrafiltration membrane, nanofiltration membrane, macroporous adsorption resin, non-ionic polymer adsorbent, muaj chromatographic kem, txav txaj chromatographic cais system thiab multifunctional chromatographic sib cais system koom nrog hauv txoj kev tam sim no invention tuaj yeem xaiv los ntawm cov khoom lag luam txawv teb chaws thiab hauv tsev.

Cov txheej txheem ntawm kev tsim muaj cov txheej txheem ntawm lub tshuab nqus tsev los yog cov txheej txheem ua yeeb yaj kiab evaporation concentration, feem ntau yog 42 degree ~ 80 degree, qhov kub zoo tshaj yog 45 degree ~ 65 degree; Kev siv tshuaj tsuag kom qhuav cov txheej txheem siv nyob rau hauv qhuav ntauwd inlet cua kub yog feem ntau 125 degree ~ 285 degree, qhov zoo tshaj plaws inlet cua kub yog 135 degree ~ 185 degree.

Lwm txoj hauv kev ntawm kev ziab ziab tuaj koom nrog cov txheej txheem ntawm qhov kev tsim tawm tam sim no suav nrog kev ziab khaub ncaws li niaj zaus, los yog airflow ziab, fluidized txaj kom qhuav, microwave nqus plua plav ziab, microwave ziab, nqus kom qhuav los yog cua kub cua kom qhuav, qhov ziab kub yog feem ntau 50 rau 100 degree, qhov zoo tshaj plaws yog 58 txog 80 degree.

Cov hmoov los yog qhuav mullein monomer compound uas tsim los ntawm cov txheej txheem ntawm kev tsim khoom tam sim no tuaj yeem siv ncaj qha los ua cov khoom siv tshuaj, lossis cov khoom siv tshuaj pleev ib ce, lossis cov khoom siv raw lossis cov khoom siv raw los tsim cov khoom noj qab haus huv xws li cov kua hauv qhov ncauj, tshuaj ntsiav, ntsiav tshuaj, granule, punch, ntsiav tshuaj lossis hnab ntim tshuaj yej. Kev txhawb nqa thiab kev siv cov txheej txheem ntawm qhov kev tsim kho no yuav ua rau cistanche tubulosa, cov tshuaj suav muaj txiaj ntsig zoo, muaj txiaj ntsig zoo rau tib neeg kev noj qab haus huv.

Cov feem pua ​​​​(feem pua) ntawm ntau cov nyiaj koom nrog hauv txoj kev tsim khoom tam sim no yog qhov hnyav feem pua ​​tshwj tsis yog hais tias lwm yam.

Tshwj xeeb embodiments

Lub invention tam sim no tau piav qhia hauv qab no ntxiv nrog rau cov embodiments.

Piv txwv 1:

1, xaiv cov txiv hmab txiv ntoo tshiabcistanche tubulosaMuab tso rau hauv qhov chaw txias txias ntawm qhov kub ntawm 4 degrees, yaug lawv hauv lub tank ntxhua khaub ncaws nrog dej txias ntawm qhov kub ntawm 18 degrees, tuaj tos lawv, muab tso rau hauv ib qho kev lag luam crushing thiab sib tsoo cov cuab yeej los tsoo thiab zom cov nqaij stems. cistanche tubulosa, ntxiv cov dej ntshiab huv los ntxuav lub hlwb kom uacistanche tubulosacrushed cell pulp, tus nqi ntawm cov dej ntxiv yog 300 feem pua ​​ntawm qhov hnyav ntawm lub hlwb crushed; Tom qab ntawd siv lub tshuab lim xovxwm Qhov kub ntawm cistanche tubulosa crushed cell pulp thiab cell pulp thaum lub sij hawm crushing thiab sib tsoo thiab sib cais txheej txheem raug kuaj kom 20 degree;

2, kub inactivation ntawmcistanche tubulosacell slurry los ntawm cov khoom siv microwave cua sov, qhov kub yog 100 degree, lub sij hawm yog 3min; Lub inactivated cistanche tubulosa cell slurry yog siv los ua cov tshuaj tiv thaiv catalytic substrate, ib txwm muaj kev tsim tawm ntawm lub cev adsorption txoj kev hydroxyapatite raws li cov txheej txheem adsorbent fixation kom tau -glucosidase immobilized enzyme raws li biocatalyst, los ntawm ib txwm immobilized txaj reactor rau cov tshuaj tiv thaiv catalytic. tawm tsis tu ncua nyob rau hauv lub immobilized txaj reactor rau catalyze tshem tawm ib tug qabzib pab pawg neeg ntawm qhov kawg ntawm cov glucosyl ligand ntawm lub substrate pinealoside thiab lub hom phiaj hloov dua siab tshiab rau cov monomeric compound ntawm mullein; Cov txheej txheem catalytic tau ua tiav ntawm qhov kub ntawm 46 degree, pH ntawm 4.9, thiab lub sijhawm catalytic cov tshuaj tiv thaiv yog 5.5h ib batch, thiab 10 pawg ntawm cov tshuaj tiv thaiv catalytic tau ua tas li; qhov kev ua -glucosidase hauv cov reactants thaum lub sij hawm catalytic cov txheej txheem yog 10U / g; los ntawm kev ntsuam xyuas tias kev hloov pauv ntawm pineal glucoside mus rau myricetin monomer compound hauv substrate mus txog 94 feem pua;

3. Cov tshuaj tiv thaiv tom qab cov tshuaj tiv thaiv catalytic saum toj no tau sib cais los ntawm ultrafiltration membrane nrog cov txheeb ze molecular loj ntawm 4000, thiab tom qab ntawd nanofiltration los ntawm nanofiltration membrane nrog cov txheeb ze molecular loj ntawm 250, tom qab adsorption sib cais los ntawm industrial macroporous adsorption resin ntawm hom AB { {3}}, thiab sib cais thiab purification los ntawm industrial chromatographic kem sib cais thiab purification txheej txheem, thiab ces ntxiv kev sib cais thiab purification los ntawm XZ12E-4L multifunctional chromatographic sib cais system kom tau siab dua purity extract ntawm mullein monomer compound;

4, cov extract saum toj no yog concentrated los ntawm industrial nqus concentration txheej txheem ntawm ib tug kub ntawm 60 degree kom tau ib tug concentrate nrog 42 feem pua ​​solids; ntxiv kom qhuav los ntawm cov txheej txheem tshuab nqus tsev ziab ntawm qhov kub ntawm 58 degree kom tau txais cov mullein monomer compound; Qhov feem pua ​​​​purity cov ntsiab lus ntawm mullein monomer compound nyob rau hauv cov khoom qhuav qhuav (hauv cov khoom qhuav) yog 87.5 feem pua ​​​​raws li kev ntsuam xyuas.

Piv txwv 2:

1. xaiv cov txiv hmab txiv ntoo tshiab ntawm cistanche tubulosa tso rau hauv qhov chaw txias txias ntawm qhov chaw cia txias ntawm 10 degree, yaug lawv hauv dej ntxhua khaub ncaws nrog dej txias ntawm qhov kub ntawm 4 degree, tuaj tos lawv, muab tso rau hauv ib qho kev lag luam crushing thiab Cov cuab yeej sib tsoo kom zom thiab zom cov nqaij ntshiv ntawm cistanche tubulosa, ntxiv cov dej ntshiab ua ntej chilled rau hauv dej kub ntawm 4 degree los ntxuav cov hlwb kom ua cistanche tubulosa crushed cell slurry, nrog rau cov dej ntxiv yog 200 feem pua. lub luj ntawm lub crushed hlwb Lub slurry yog ces sib cais los ntawm ib tug industrial centrifuge kom tau cistanche tubulosa cell slurry; thiab cov lim residue tau ntxiv rau cov dej ntshiab huv, nrog rau cov dej ntxiv yog 200 feem pua ​​​​ntawm qhov hnyav ntawm cov lim seem, thiab ntxiv hauv av rau hauv slurry los ntawm kev lag luam niaj hnub crushing thiab sib tsoo tshuab, thiab cov hlwb crushed tau muab rho tawm los ntawm ib qho ultrasonic extraction ntaus ntawv, thiab ces sib cais los ntawm ib tug muaj centrifuge mus ntxiv tau cistanche tubulosa cell slurry; Tom qab kev sim, kev sib tsoo thiab kev sib tsoo thiab kev sib cais Qhov kub ntawm cistanche tubulosa crushed cell pulp thiab cell pulp yog 18 degree;

2, kub kub inactivation ntawm cistanche tubulosa cell slurry los ntawm industrial cua sov ntaus ntawv, qhov kub yog 100 degree, lub sij hawm yog 5min; Lub inactivated cistanche tubulosa cell slurry yog siv los ua cov tshuaj tiv thaiv catalytic substrate, ib txwm muaj kev tsim khoom ntawm lub cev adsorption cellophane raws li cov txheej txheem adsorbent fixation, kom tau txais -glucosidase immobilized enzyme raws li biocatalyst, los ntawm cov niaj hnub muaj immobilization reactor rau nruam hom Catalytic. Cov tshuaj tiv thaiv tau ua tiav ntawm 45 degree, pH 4.5, thiab lub sijhawm catalytic cov tshuaj tiv thaiv yog 6.0h ib batch, thiab 12 pawg ntawm cov tshuaj tiv thaiv catalytic tau ua tas li. Kev hloov pauv ntawm pineal glucoside rau hauv myricetin monomeric compound hauv substrate tau kuaj pom li 95 feem pua;

3, cov tshuaj tiv thaiv tom qab cov tshuaj tiv thaiv catalytic saum toj no yog nanofiltration siv nanofiltration membrane nrog ib tug txheeb ze molecular loj ntawm 300, thiab ces sib cais los ntawm adsorption siv ib tug industrial macroporous adsorption resin ntawm XAD hom, thiab purified los ntawm ib tug industrial chromatographic kem sib cais thiab purification txheej txheem , thiab tom qab ntawd ntxiv kev sib cais thiab ua kom huv si siv ntau lub tshuab chromatographic sib cais ntawm XZ20Z-ZL hom kom tau txais ntau dua purity extract ntawm mullein monomer compound;

4, cov extract saum toj no tau dhau los ntawm kev lag luam zaj duab xis evaporation concentration ntawm 55 degree kom tau txais cov concentrate nrog 45 feem pua ​​​​cov ntsiab lus khib nyiab; ntxiv dhau los ntawm kev lag luam nruas kom qhuav ntawm 55 degree kom tau txais Myristica fragrans monomer compound; Tom qab kev tshuaj xyuas thiab kev txiav txim siab, qhov feem pua ​​​​purity cov ntsiab lus ntawm Myristica fragrans monomer compound nyob rau hauv cov khoom qhuav qhuav (hauv cov khoom qhuav) yog 89.9 feem pua.

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