Urric acid Txo cov nyhuv thiab kev siv tshuab ntawm kev ua yeeb yam thiab echinacoside extracted los ntawm cistanche tubulosa
Mar 03, 2025
3 tau tshwm sim
3.1 Qhov cuam tshuam ntawm raws li thiab Ech ntawm urea, crea, sua, thiab uua hauv hyperururicemia qauv nas
Raws li qhia hauvDaim duab 1, piv nrog cov pawg ib txwm tswj, cov hyperuricemia qauv nthuav dav ntau ntau qib ntawm urea, crea, thiab sua (p<0.05 or P<0.01) and significantly decreased levels of UUA (P<0.01). Compared with the model control group, the AS low-dose, AS high-dose, and ECH high-dose groups showed significantly reduced levels of UREA, CREA, and SUA (P<0.05 or P<0.01) and significantly increased levels of UUA (P<0.05 or P<0.01). In addition, the ECH low-dose group showed a significant reduction in UREA levels (P<0.05 or P<0.01).
Daim duab 1 Teeb meem ntawm ASEA, Crea, Sua, Sua Sua, thiab UA S, N {2}}) #p<0.05, ##P<0.01 vs Control group; *P<0.05, **P<0.01 vs Model group.
Tshuaj ntsuab cistancel ntxiv nrog qib siab thiab raws li
3.2 Cov teebmeem ntawm AS thiab E NECH ntawm ADA thiab XOD hauv Hyperuricemia Model nas
Raws li qhia hauvDaim Duab 2, piv nrog pab pawg kev tswj hwm ib txwm muaj, cov hyperuricemia qauv nthuav dav theem ntawm ADA thiab XOD (P<0.01). Compared with the model control group, the allopurinol group, AS low-dose and high-dose groups, and ECH low-dose and high-dose groups showed significantly reduced levels of ADA and XOD (P<0.05 or P<0.01).
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Daim duab 2 Teeb Meem ntawm AS thiab E NECH ntawm ADA thiab XOD hauv Hyperuricemia Model Model (X -}}}) #p<0.05, ##P<0.01 vs Control group; *P<0.05, **P<0.01 vs Model group.
3.3 Qhov cuam tshuam ntawm AS thiab E NECH ntawm lub raum coefficients hauv nas
Raws li qhia hauvDaim Duab 3, piv nrog pab pawg ib txwm tswj, cov hyperuricemia qauv tswj hwm pom tau cuam tshuam loj dhau lub cev hnyav (P<0.01) and significantly increased kidney weight and kidney coefficient (P<0.01). Compared with the model control group, the AS high-dose group and the ECH low- and high-dose groups showed significantly increased fasting body weight (P<0.01 or P<0.05). In the allopurinol group, AS low- and high-dose groups, and ECH low- and high-dose groups, kidney weight and kidney coefficient were significantly reduced (P<0.01).
Daim duab 3 Qhov tshwm sim ntawm AS thiab E NECH rau ntawm lub raum coefficient hauv hyperuricemia Model nas (X -}}) #p<0.05, ##P<0.01 vs Control group; *P<0.05, **P<0.01 vs Model group.
3.4 Patholhological soj ntsuam ntawm cov teebmeem ntawm raws li thiab Ech txog raum hauv hyperuricemia qauv nas
Raws li qhia hauvDaim Duab 4, cov pawg ib txwm tswj tau nas ua rau pomli niaj zaus glomerular morphology, nrog cov qauv qub nyob rau hauv Renal tubules thiab intererstwium. Piv nrog cov pawg tswj hwm ib txwm muaj, Pawg Qauv Txheej Txheem Xaiv tau ntawm lub raum, thiab mob cell in inflidator hauv lub raum intererstsis. Piv nrog rau pawg qauv, tag nrho cov pab pawg tagnrho Nyob rau hauv cov pab pawg kho mob thiab Ech pab pawg, qee cov nas pom pom tias ib txwm muaj nyob rau hauv Renal lub cev In Infilmatory IntererstWlium tau raug txo. Ib qho ntxiv, epithelial cell shedding, necrososis, thiab mob hlwb ntawm lub cev tsis muaj kev cuam tshuam.
Daim duab 4 Nws cov duab ntawm cov lej ntawm cov renal (× 200) A: Pawg pab pawg; B thiab C: qauv pawg; D: Allopurinol pab pawg; E: Raws li 50 mg -1 pawg, f: xws li 100 mg {}} mg eceb -1 pab pawg.
Daim duab 5 immunohistochemical staining ntawm cov nqaij liab hauv hyperuricemia qauv nas (IHC, × 400)
Daim duab 6 Teeb Meem ntawm AS thiab ECH ntawm cov protein qhia nyob rau hauv lub raum nqaij ntawm hyperuricemia qauv nas (x -}}) #p<0.05, ##P<0.01 vs Control group; *P<0.05, **P<0.01 vs Model group.
Daim duab 7 Qhov cuam tshuam ntawm AS thiab ECH ntawm Cov Gene cov nqaij daim ntaub so ntswg hauv hyperuricemia qauv nas (x -} {2}}) ## p<0.01 vs Control group; **P<0.01 vs Model group.
4 kev sib tham
Kev txhim kho ntawm hyperuricemia (Hua) yog txuam nrog kev siv kua ricid ntau dhau thiab tsis txaus ntseeg urric acid excretion. Hauv txoj kev tshawb fawb no, peb tsim cov qauv hyperuricemia hauv cov nas siv kev sib xyaw ua ke ntawm Adenine thiab Potassium Oxonate, raws li tau tshaj tawm hauv cov ntawv nyeem. Cov txheej txheem no nce siab uric cov kua qaub thaum txo nws cov excretion, kom haum rau cov pib ua haujlwm ntawm hyperuricemia. Cov txiaj ntsig kev sim ua tau pom tau tias yog tsim kom muaj tus qauv huua huua tau ruaj khov. Nyob rau hauv pab pawg kho, muaj ntau yam ntawm cov zis roj av, thiab cov nas roj a Cov kev tshawb pom no qhia tau tias muaj thiab Ech, Phenyylethanoid glycoside extractions los ntawmCistanche tubulosa, tuaj yeem txhim kho lub raum muaj nuj nqi, txhawb kho acid excretion, thiab txo cov qib acid hauv Hua qauv nas.
Xanthine oxidase (xd) thiab Adenosine Deaminase (ADA) yog cov enzymes tseem ceeb koom nrog hauv kev tsim tawm hauv lub siab. Xod ncaj qha tswj kev siv olic acid ntau lawm los ntawm oxidizing kev hloov pauv los ntawm nucleoside phosphorline thiab xod [12-13]. Uric acid excretion tshwm sim feem ntau ntawm cov raum thiab kev sib tw pidwing, nrog kwv yees li ob feem peb ntawm cov kua txob txhua hnub los ntawm lub raum. Yog li ntawd, puas lub raum Uric acid excretion yog suav hais tias yog ib qho ua rau muaj kev kub ntxhov ntawm hyperuricemia [14-15]. Protein koom nrog urric acid reaborption muaj xws li UTAT1 thiab Glut9. Cov kev tshawb fawb tau pom tias koj reasrotorption yog kho kom haum los ntawm kev koom tes ua haujlwm ntawm URAT1 thiab glut9 protein [16]. Thiaj li, Urat1 thiab Glut9 yog cov phiaj tseem ceeb rau hyperuricemia tshawb nrhiav thiab kev txhim kho cov kua qaub-ua kom tsawg. OAT1 feem ntau qhia nyob rau hauv cov basolateral membrane ntawm proximal raum liab ceg thiab ua lub luag hauj lwm tseem ceeb nyob rau hauv uptakes rau lub raum tubules los ntawm cov chaw insterstittite [17]. OAT3 yog cov Urate Transporter Protein feem ntau tau muab tso rau hauv cov basolateral memules thiab koom nrog hauv ipheral tubules, pab txhawb uric acid zais zais [18]. Ua ke, OAT1 thiab OAT3 kho tric acid thauj hauv lub raum ntawm ion-dicarboxylate pauv, ua rau lawv lub hom phiaj tseem ceeb rau kev kawm ionic acid excretion.
Kev sim ua kom pom tias ob yam li thiab Ech txo ADA thiab XO QIB, nrog rau cov tshuaj tiv thaiv Urat1 thiab GLA ntawm lub raum nqaij ntawm Hua tus qauv nas. Tsis tas li ntawd, lawv nce qib kev tshaj tawm ntawm OAT1 thiab OAT3 protein thiab mRNA. Cov kev tshawb pom no qhia tau hais tias Uric acid-txo cov kev cuam tshuam ntawm cov khoom siv ece acid-salthizes, thiab tswj kav cov kab ke ntawm cov lus tso zis. Tsis tas li ntawd, tsis muaj ib txoj kev sib txawv tseem ceeb tau pom nruab nrab ntawm cov pab pawg kho mob hauv lawv cov teebmeem ntawm ADA, XYR qib, thiab Urate Transporter qhia.
Qhov kev kawm no qhia tias ob qho tib si xws li thiab Ech nthuav tawm ecic acid-txo cov teebmeem, uas yuav cuam tshuam nrog lawv cov qauv tshuaj sib xws. Raws li yog cov dej-soluble phenylethanoid glycoside glycoside plycoside cov pachaw uas sib xyaw ua ke nrog dej qab zib thiab cov piam thaj glycosidic. Ech yog cov sib xyaw ua ke ntawm phenylropanoid thiab phenylethanoid glycosides txuas rau lub trisaccharide. Ob qho sib xyaw ua ke qhia cov phenylethanoid glycoside qauv thiab zwm rau cov phenyylethanoid glycoside chav kawm. Phenylethanoid glycosides yog feem ntau muaj cov phenyylethanol thiab qab zib moog. Ob qho tib si thiab ov muaj qab zib maniaeties, uas txhim kho lawv cov dej solubility thiab bioactivity. Tsis tas li ntawd, ob qho tib si thiab Ech muaj cov nplhaib benzene nrog ntau yam hloov chaw uas cuam tshuam lawv cov khoom lag luam thiab cov khoom siv dag zog.
Hauv kev xaus, phenylethanoid glycoside muab rho tawm raws li thiab Ech los ntawmCistanche tubulosaTso cai uric acid-txo cov kev cuam tshuam hauv Hua tus qauv nas tsim nrog Adenine thiab Potassium oxonate, yuav vim lawv cov qauv tshuaj sib xws. Lawv cov tshuab ntawm kev nqis tes ua yuav suav nrog kev txhim kho raum ua haujlwm, txo cov kua qaub ineulating, thiab tswj cov kev hais tawm ntawm cov zis.
