Unveiling cov txheej txheem rho tawm ntawm cov khoom muaj txiaj ntsig zoo los ntawm cistanche Squaticola

Raws li ib tug precious tsoos tshuaj suav tshuaj nyob hauv Suav teb, cov txheej txheem rho tawm ntawm cov khoom xyaw nquag los ntawm cistanche Squaticola tau nyiam ntau. Nyob rau hauv cov lus qhia, cia thawj nkag siab qhov tseem ceeb ntawm cistanche Showicola. Cistanche Squaticola tau sau tseg hauv "Shennong Bencao Jing" thiab tau cais raws li cov tshuaj ntsuab saum toj no. Nws muaj qhov cuam tshuam ntawm lub rooj ob ob lub raum thiab yaj, replenishing essence thiab melwe, noj kom muaj ntshav thiab noo lub neej. Nws yog tsuas yog siv los kho txiv neej impotence, poj niam muaj menyuam yaus, chaw mos vau, txias mob hauv lub duav thiab cem, thiab cem quav los ntawm ntshav tsis txaus. Nws tsuas yog loj hlob nyob hauv cov chaw suab puam xws li sab hnub poob sab hauv Mongolia, Gansu, thiab yog ib qho tshuaj suav suav suav nrog hauv Suav papaceplopoeia.
Cov tshuaj ntsuab tshuaj ntsuab cistanche Squaticola muaj cov txheej txheem bietethanol, tsis quav ntsej lwm cov khoom siv, ua rau muaj cov khoom pov tseg tseem ceeb. Txhawm rau kom daws tau cov teeb meem no, cov kws tshawb fawb ntau tau cog lus los kawm cov txheej txheem rho tawm ntawm cistancy siv ntawm cistancy cov khoom siv.

Desert Nyob Cistanche

Cov khoom xyaw nquag ntawm cistanche Squaticola

Raws li lub ntsej muag zoo nkauj suav tshuaj, cistanche Squaticola muaj ntau yam khoom siv xyoob. Ntawm lawv, phenylethanoid glycosides yog ib qho ntawm cov khoom sib txawv ntawm cistanche nquag, nrog ntau cov txiaj ntsig tshuaj. D-mannitol tseem muaj dav tam sim no nyob rau hauv cistanche Squaticola thiab muaj cov tshuaj lom neeg ntawm polyol. Nws yog dav siv nyob rau hauv daim teb xws li tshuaj, khoom noj, thiab textiles. Tsis tas li ntawd, Polyaccharides tseem yog ib qho ntawm cov khoom tseem ceeb ntawm Cistanche Showicola, nrog cov haujlwm xws li kev tswj kev tiv thaiv kab mob. Thiab betaine muaj cov ntsiab lus siab hauv cistanche Squaticola, nrog ntau yam kev ua pharmacological.
Tam sim no, muaj ntau txoj hauv kev rau kev rho tawm cov khoom xyaw zoo los ntawm cistanche Squaticola. Piv txwv li, kev siv hydrolicygatic ua ke nrog cov kev rho tawm ultrasound yuav siv tau los rho tawm polysaccharides los ntawm cistanche Squarance. Cov kauj ruam tshwj xeeb yog raws li hauv qab no: rho tawm cov crude hmoov ntawm cistanche Squaticola nrog Ethyl acetate thiab ethanol nyob rau hauv cov txheej txheem, sau cov seem kom tau txais kev txiav txim siab cistatted cistanticola hmoov; Ntxiv papain, cellulase, thiab pectinase kom defatted cistanche shouldicola hmoov, ntxiv}. 75 teev, {6 {6 {6}} lub sij hawm. Ua ke cov filtrate, centrifuge tus supernatant thiab mloog nws. Tom qab ntawd ntxiv ethanol mus rau lub ethanol rau lub ethanol concentration yog 80%, cia nws sawv ntsug, centrifuge kom tau txais ib nag lossis khov kho kom qhuav.

Cistanche Deetricicola sim

Kuj tseem muaj ib txoj kev rho tawm polysaccharides los ntawm cistanche verticanca los ntawm kev sib xyaw ntawm enzyme complex thiab ultrasonic rho thev naus laus zis. Cov txheej txheem zoo tshaj plaws rau cov polysaccharides los ntawm Cistanche cov khoom siv ntawm 450, cov khoom siv sib xyaw ua ke) ntawm 1, Qib 5.14% Tomqab peb cov kev rho tawm rov qab, nrog qhov feem pua ​​ntawm 90.78% thiab cov ntsuas ntsuas qhov ntsuas ntawm 47.96%. RSD yog 1.51%, qhia tau qhov ua kom zoo tshaj plaws reproppility.
Ib qho ntxiv, Orthorogonal kev sim tsim kuj tseem siv tau los kawm cov txheej txheem rho tawm ntawm cistanche Squaticola. Kawm cov yam uas cuam tshuam rau cov txheej txheem rho tawm ntawm cov txheej txheem betaine siv betaine nyob rau hauv extraction daws raws li qhov ntsuas kev ntsuas. Ntawm plaub yam hauv kev sim tsim, orthanol cov kev xav tau muaj qhov cuam tshuam tseem ceeb. Cov txheej txheem zoo tshaj plaws rau cov txheej txheem Neicong yog: Reflux Extraction nrog 75% ethanol ntawm 8 zaug, txhua zaus rau 1 teev.
Ultrasonic rho tawm thev naus laus zis kuj tseem siv dav hauv kev rho tawm cov khoom xyaw nquag los ntawm cistanche Squaticola. Thaum rho tawm polysaccharides los ntawm cistanche Showicola siv dej, ultrasonic extraction extraction extraction rau 24 teev thiab 100 degree reflux rau 6 teev. Thaum rho tawm betaine los ntawm cistanche Showicola siv cov dej, ultrasound extraction rau 20 teev thiab 100 degree reflux rau 6 teev. Thaum rho tawm glycosides los ntawm cistanche Showicola siv methanol, ultrasonic rho tawm rau kev rho tawm ib qho ntxiv li cov tshuaj ntxiv rau 24 teev thiab ua kom ntev li 5 teev.
Ib txoj hauv kev rau ntsev ua rau cov phenylethanoid glycosides los ntawm cistanchoariticola siv qhov tsawg me me taw qhia txog cov kuab tshuaj aqueous aqueous. Cov hmoov ntawm cistanche Squaticola tau muab rho tawm siv lub zog tsis zoo, thiab theem ob tau npaj los ntawm kev daws teeb meem ntawm cov pa phenymoid glycosides los ntawm cistanche Squarance. Kev rho tawm thiab cov chaw ib puag ncig kuj yog qhov sib cais me me, thiab cov txiaj ntsig ntawm cov phenylethanoid glycosides hauv cistanchoarma Squaticola tuaj yeem mus txog 20%.

cistanche hmoov

Cov kev rho tawm ntawm mannitol los ntawm cistanche Squaticola tuaj yeem yog kev kawm thiab ntau rau kev kawm siv infrared spectroscopy thiab spectrophotometry. Cov txheej txheem rho tawm ntawm d-mannitol los ntawm cistanche Squaticola tau npaj tseg, thiab cov hmoov dawb mankitol muaju tau txais los ntawm crystallization txoj kev. Cov txiaj ntsig ntawm Munitol tau txais los ntawm cistanche Squaticola momnitol rho cov txheej txheem yog 2.9%.
Cov txheej txheem rho tawm ntawm cov khoom muaj txiaj ntsig zoo hauv contanche Squaticola tuaj yeem ua tiav los ntawm kev ua kom qhuav, thiab kom qhuav kom tau txais Cistanche Squaranceola D-Mannitol; Extract qhov seem nrog cov tshuaj ethanol nrog lub tswv yim ntawm {2}}%. Cov kev rho tawm yog dhau los ntawm thaj chaw txua nqa hluav taws xob, thiab cov neeg eluent yog concentrated thiab qhuav kom tau penylethanmolic glycosides ntawm cistanche Squarance; Cov khoom seem qis dua yog ntxiv kom sov thiab muab rho tawm nrog dej distilled. Tus supernatant yog feeb txog kev txo qis thiab tom qab ntawd precipitated nrog 3 zaug ntim ntawm 95% ethanol. Cov nag los yog qhuav kom tau polysaccharides los ntawm cistanche Squarance.
Cov txheej txheem extraction ntau

Cov txheej txheem rho tawm ntawm cov khoom xyaw ua haujlwm hauv Contanche Squarance yog nplua nuj thiab cov hauv qab no yog ntau txoj kev sib txuam.
Tsoos ethanol cov extraction ua ke nrog macroporous resin sib cais txoj kev
Ua ntej, cov tshuaj ntsuab tshuaj ntsuab cistancho voventicola yog qhuav ntawm 60 degree thiab crushed rau 40 mesh.
Tom qab ntawd, 90-95% kev daws teeb meem tau siv los tshem tawm cistanche concenchate, thiab qhuav kom tau txais cistanche Squanticola d-mannitol.
Tom qab ntawd, chav tshem tawm cov khoom seem tau muab rho tawm siv ethanol daws nrog lub ntim piv rau 60-80%. Cov rho tawm tau dhau los ntawm thaj chaw txua nqa hluav taws xob macroporous, thiab tus eluent tau sau. Tom qab cov pej xeem thiab ziab, cov phenylethanolic glycosides ntawm cistanche Showicola tau txais.
Thaum kawg, cov seem tom qab rov rho tawm tau ntxiv rau cov dej distilled thiab rhuab rau extraction. Tus supernatant tau feeb meej nyob rau hauv kev txo siab thiab precipitated nrog 3 zaug ntim ntawm 95% ethanol. Tom qab ziab, cov polysaccharides los ntawm Cistanche Showicola tau txais.

cistanche extract hmoov

Macroporous Rogram sib cais thiab txoj kev ua kom huv
Zom cov cistanche Squaticola thiab sib tov nws nrog ib tug ethanol daws nrog cov feem pua ​​ntawm 50-70%. Thaum tshav kub kub thiab reflux rau extract 1-3 lub sij hawm, txhua lub sijhawm rau {2}} teev. Lim thiab mloog cov filtrate.
Cov dej cawv tsis zoo los ntawm cistanche Squaticola tau dhau los ntawm thaj chaw ua haujlwm macroporole thiab eluted nrog dej thiab ethanol daws hauv ntu. Cov ethanol Kev daws kev daws teeb meem yog sau kom tau txais cov extract ntawm cistanche Squaticola, uas muaj tag nrho glycosides ntawm cistanche ntawm cistanche ntawm cistanche ntawm cistanche vovanche Squaranceola.
Kev sib xyaw ua ke voj voog thiab cais cov txheej txheem
Extraction ntawm tag nrho cov penylethanmolic glycosides los ntawm cistanche Showicola: Hauv qab no}}} feeb, nrog {{4 4}} extractions. Ua ke cov filtrate thiab tsom ntsoov nyob rau hauv siab txo kom tau txais cov tshuaj muaj zog I.
Sib cais ntawm tag nrho cov phenylethanol glycosides hauv cistanchoe voluticola: Ntxiv cov tshuaj ntxuav ua kom tiav rau kev khaws cia, thiab tom qab ntawd ntxuav nrog methanol lossis ethanol daws. Sau cov feem ntau cov phenylethanol glycosides, mloog zoo thiab qhuav kom tau txais tag nrho cov pistylethanol glycosides hauv cistanche vistanche Squarance.
Extraction ntawm tag nrho cov oligosaccharides thiab cov txiv polysaccharides ntawm cistanche Showicola: Muab cov feeb lim dej rau {}}}}}}}}}}}} {}} Tsom ntsoov nyob rau hauv kev txo qis kom tau txais cov tshuaj concent concent် II; Tso cov tshuaj muaj zog rau hauv chav tsev sov, ntxiv}}}}}}}}% kom tau txais tag nrho oligosaccharides ntawm cistanche Squaticola; Tom qab cov nag lossis nag lossis daus yog ib nrab qhuav, cov polysaccharides ntawm cistanche Showicola tau txais.
Lwm txoj kev sib txuam

Kev ua kom zoo ntawm kev rho tawm kev siv cov lus teb sab nraud txheej txheem sib txuas nrog cov qauv sib txawv
Siv ib qho kev ntsuas sib xyaw ua ke nrog lub thawv pom ntawm cov txheej txheem sib txawv, xws li lub sijhawm extraction ntawm cistanche, xws li lub sijhawm extraction, cov khoom siv rho tawm, thiab cov sij hawm rho tawm. Tom qab ntawd, los ntawm kev sim tsim, nrhiav cov kev ua tau zoo. Tom qab kev ua kom pom kev zoo, HPLC tau siv los xyuas cov ntsiab lus ntawm cov ntsiab lus xws li Echinacroside hauv Cistanche Squarance hauv cov hau kev sib txawv. Los ntawm cov hau kev xws li cluster kev tsom xam, thiab kev tshuaj xyuas tus nqi ntawm cistances ntawm cistanche kev ntsuas tus nqi sib txawv tau ua rau xaiv cov txheej txheem kom qhuav. Cov ntaub ntawv siv xws li "kev ua kom zoo ntawm cov txheej txheem phenyletholma", "kev ua kom zoo dua ntawm cistanchourging ntawm cistancho cov txheej txheem ntawm cenranticola los ntawm kev teb cov lus qhia ntawm cenranchology", " Cov txheej txheem hauv kev rho tawm cov khoom xyaw nquag los ntawm cistanche Squaticola, muab kev paub ua ntej thiab cov kev paub ua haujlwm rau kev siv cov qauv no.

cistances 200mg

Kev npaj ntawm cistanche Squaticola pulp los ntawm kev rho tawm ancient thiab enzymatic hydrourysis
Kev rho tawm cov tshuaj zoo ntawm cov tshuaj suav ntawm cistancho ntawm cistancho vostricola puree, xws li kev zoo siab cistancho Showicola Puree. Nrog kev pab los ntawm cov khoom siv roj ntsha niaj hnub, cov khoom siv raw no tuaj yeem txhawb cov khoom xyaw uas nquag ntawm cistanche Squaticola, nrog kev nqus txog li 99.9%. Cov txheej txheem npaj tau muaj xws li tu cov khoom tsis huv tshiab, thiab tshem dej noo kom tau txais cov khoom lag luam ua ntej; Hlais thiab zuaj cov khoom lag luam kom tau txais cov khoom siv; Twj tso kua mis cov khoom mus rau lub tshuab lim dej thiab cais cov tawv nqaij slag nrog ib sab cib kom tau txais cov slurry; Ua cov tshuaj tiv thaiv kab mob antioxidant ntawm slurry, ntxiv citric acid qhuav hmoov thiab vitamin C Qhuav Hmoov, thiab kho tus nqi PHIC; Tso tshuaj tua kab mob tawm tsam slurry rau hauv ib lub zeb sib tsoo rau kev sib tsoo; Homogenize av slurry nyob rau hauv kev kub siab kom rhuav lub xovtooj ntawm phab ntsa; Thaum kawg, ua pasteurization thiab sau. Cov ntaub ntawv siv xws li "Kev npaj ua qauv ntawm cistances thiab" kev npaj cov khoom siv rau hauv cantey "
Ua ke ntau yam niaj hnub rho tawm cov txheej txheem
Cov txheej txheem extraction niaj hnub xws li cov tshuaj chromatography, thiab siv cov tshuaj xyaw chromatography, thiab kev pab cuam hauv chromatography-ultrasound ntawm cov khoom xyaw nquag hauv cistancho verticola. Cov kev ua kua dej supercriterical cov txheej txheem siv cov kua dej uas muaj roj carbon dioxide xws li cov roj carbon dioxes los ntawm cov khoom siv rau hauv lub xeev cistancho hauv lub xeev. Nws muaj qhov zoo ntawm kev rho tawm cov kev txuag siab, muaj zog xaiv, thiab kev puas tsuaj tsawg rau cov thermosensitive Cheebtsam. The microwave-assisted extraction method utilizes the thermal and non thermal effects generated by microwave radiation to promote the rupture of the cell wall of Cistanche deserticola, accelerate the release of effective ingredients, and achieve fast and efficient extraction speed. Txoj kev pab ultrasound extraction cov cavitation thiab cov neeg siv khoom siv ntawm ultrasound los rhuav tshem lub cell phab ntsa ntawm cistanche Showicola thiab txhawb nqa cov khoom xyaw nquag. Nws tseem muaj cov yam ntxwv ntawm kev nrawm nrawm nrawm thiab ua haujlwm siab. Kab chromatography thiab kev ua tau zoo ua kua chromatography siv cov tshuaj sib txawv ntawm cov tshuaj sib cais, thiab cov theem sib cais ntawm cov khoom sib cais zoo, thiab kev xaiv siab, thiab muaj zog xaiv. Cov ntaub ntawv siv xws li "extraction thiab sib cais ntawm cov ntsiab lus zoo hauv cistancients hauv cistancients hauv cistancents hauv cistanche, muab cov ntaub ntawv nthuav tawm cov khoom siv tau zoo hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv zoo niaj hnub no, muab cov ntaub ntawv nthuav tawm cov qauv zoo hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv zoo hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv zoo hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv zoo hauv cistanche, muab cov ntaub ntawv nthuav tawm cov qauv zoo hauv cistanche, muab ib qho kev siv rau kev siv rau ntau cov kev sib xyaw.
tag

Muaj ntau cov txheej txheem rho tawm rau cov khoom xyaw nquag ntawm cistanche Squaticola, thiab cov kev sib txawv uas muaj lawv tus kheej thiab qhov tsis zoo. Hauv kev siv cov tswv yim, cov txheej txheem tiv thaiv haum tuaj yeem raug xaiv raws li qhov kev xav tau los siv txiaj ntsig zoo ntawm cistanche Squarance.
Kev cais tawm ntawm cov txheej txheem macroporous thiab lwm cov txheej txheem sib txawv, muab cov kev sib cais ua kom zoo dua qub, thiab cov kev sib xyaw ua ke rau kev rho tawm ntawm Cov khoom xyaw zoo los ntawm cistanche Squaticola.
Piv txwv li, cov tshuaj ib txwm muaj kev rho tawm ua ke nrog cov txheej txheem mankitolic, thiab polysacintides, thiab cov kauj ruam ntawm cov qauv no yog qhov sib txawv. Cov macroporous rogram sib cais thiab txoj kev ua kom huv yog qhov yooj yim rau kev ua haujlwm, tab sis tej zaum yuav muaj qhov sib txawv me ntsis hauv kev txuag hluav taws xob. Kev sib koom ua ke rau ultrasonic kev rho tawm thiab kev sib cais siv cov tshuab siv ultrasonic los txhim kho cov kev ua kom muaj zog, tab sis yuav tsum muaj cov cuab yeej siv cov khoom muaj nqis.

Cov tshuaj loj ntawm cistancho squaticola

Nyem qhov no mus saib cistranche txhim kho cov khoom siv tiv thaiv kev tiv thaiv

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Teb sab nraud txheej txheem optimolity rho tawm cov txheej txheem sib xyaw ua ke los ntawm cov qauv kev sim, thiab kev ua kom zoo tshaj plaws cov ntsiab lus kom qhuav thiab zoo ntawm cov khoom xyaw nquag. Txoj kev coj ua ib txwm muaj nrog cov roj ntsha enzymatic hydrocysis siv los tsim cov thawj ntawm cistancho verticola. Nrog kev pab los ntawm cov kev pab cuam muaj xyoob ntoo muaj txiaj ntsig, cov khoom siv ua tau zoo ntawm cistanche Squaranceola tuaj yeem ua rau muaj tus nqi sib haum xeeb, tab sis tus nqi yuav muaj siab. Kev sib xyaw ua ke ntawm ntau cov txheej txheem rho tawm cov kua dej rho tawm, microwave-pab extraction effension, tab sis cov kev xaiv ua kom muaj zog, tab sis cov kev paub ua haujlwm thiab tus nqi kuj yuav siab.
Hauv cov ntsiab lus, thaum xaiv cov txheej txheem rho tawm rau cov khoom xyaw uas nquag siv los ntawm cistancy kev siv tau ntawm cistancy cov peev txheej ntawm cistancy cov peev txheej thiab tag nrho cov tshuaj muaj nuj nqis.