Kev kho lub luag haujlwm ntawm Mango Peels hauv Kev Tswj Ntawm Dyslipidemia Thiab Oxidative Stress hauv Cov Poj Niam Obese
Feb 21, 2022
Xav paub ntau ntxiv ntawm Emailtina.xiang@wecistanche.com
Kev rog rog yog ib qho mob metabolic thiab tsis kis kab mob uas cuam tshuam txog 50 feem pua ntawm cov neeg hauv ntiaj teb. Reactive oxygen hom thiab oxidative kev nyuaj siab yog cuam tshuam nrog kev rog rog thiab ntau yam kab mob metabolic, tau txais kev saib xyuas ntawm cov zej zog kev tshawb fawb los tawm tsam qhov teeb meem no ib txwm muaj. Ntawm ntau yam txiv hmab txiv ntoo, txiv nkhaus taw raws li cov txiv hmab txiv ntoo daj yog nplua nuj nyob rau hauv polyphenols, carotenoids, terpenes, thiab flavonoids uas ua raws li antioxidants los tiv thaiv dawb radicals tsim nyob rau hauv lub cev. Txoj kev tshawb no tau ua los tshawb nrhiav hauv vivoantioxidantLub peev xwm ntawm mango peels tawm tsam dyslipidemia thiab oxidative kev nyuaj siab hauv cov neeg rog rog. Cov poj niam tuaj yeem pab dawb (n=31) nruab nrab ntawm 25 thiab 45 xyoo uas muaj lub cev qhov ntsuas (BMI) ntawm 25.0-29.9 (hnyav) tau suav nrog hauv txoj kev tshawb no, thaum cov neeg koom nrog muaj teeb meem xws li ntshav qab zib, ntshav siab, mob plawv, thiab kab mob siab tau raug cais tawm. Cov pab pawg kho mob tau noj 1 g txiv nkhaus taw tev hmoov rau 84 hnub. Cov ntsiab lus tau txheeb xyuas rau biochemical tsom xam,antioxidantxwm txheej, thiab kev ntsuas anthropometric ntawm lub hauv paus thiab qhov kawg ntawm lub sijhawm kawm. Tsis tas li ntawd, thaum kawg ntawm txoj kev tshawb no, kev ntsuam xyuas kev nyab xeeb kuj tau ua. Cov txiaj ntsig tau pom tias thaum noj txiv nkhaus taw tev hmoov, qis lipoproteins tsawg (LDL), cholesterol, triglyceride, urea, thiab creatinine qib tau txo qis thiab qib high-density lipoprotein (HDL) tau nce (P Tsawg dua lossis sib npaug rau {{ 2}}: 05), thaum thiobarbituric acid reactive tshuaj (TBARS) pom tau nceantioxidantraws li txoj cai (P Tsawg dua los yog sib npaug rau 0:05) uas qhia tias mango peels muaj peev xwm tswj tau zoo tiv thaiv oxidative kev nyuaj siab thiab dyslipidemia hauv cov neeg rog rog.
1. Taw qhia
Kev rog rogyog ib qho kab mob ntev uas tshwm sim los ntawm kev sib sau ntawm cov rog hauv cov ntaub so ntswg adipose uas ua rau lub cev hnyav [1, 2]. Nws raug suav hais tias yog kab mob metabolic nquag, muaj 50 feem pua ntawm cov neeg laus thoob plaws ntiaj teb [3]. Nws yog qhov tshwm sim raws li qhov xav tias yuav cuam tshuam rau 38 feem pua ntawm cov poj niam laus thiab 36.9 feem pua ntawm cov txiv neej laus thoob plaws ntiaj teb [4]. Kev rog rog yog qhov tseem ceeb ua rau tus neeg sawv cev rau oxidative kev nyuaj siab, uas rov ua rau qhov xwm txheej tsis zoo los ntawm kev hloov cov metabolite ua haujlwm thiab txhawb cov txheej txheem ntawm o los ntawm cytokines [1, 5]. Tsis tas li ntawd, vim muaj zog oxidative kev nyuaj siab, innate tiv thaiv yog tshwm sim uas ua rau kom nce o thiab lipid peroxidation uas yog qhov kawg txoj kev ua rau txawv degenerative kab mob [6, 7]. Nws tau hais tias lub voj voog khiav ntawm kev rog thiab oxidative kev nyuaj siab uas muaj kev hem thawj rau cov hlwb thiab cov ntaub so ntswg, ntxiv depriving lawv ntawmantioxidants[8]. Adipose cov ntaub so ntswg ua raws li lub caj pas uas tsim cov tshuaj hormones, piv txwv li, resistin, estrogen, thiab leptin, thiab qhia cov proteins xws li cytokines. Hauv kev teb rau adipocytes ntau ntxiv, cov adipokines uas tsis tau kuaj xyuas yog zais cia uas qhia tias muaj zog tiv thaiv kab mob los ntawm kev tsim cov pa oxygen reactive (ROS) thiab dawb radicles, ntxiv dag zog rau oxidative kev nyuaj siab [9]. Tsis tas li ntawd, oxidative kev nyuaj siab ua rau cov kab mob pancreatic puas tsuaj thiab cuam tshuam rau kev tsim thiab tso tawm ntawm insulin ua rau kev hloov pauv ntawm cov piam thaj mus rau cov ntaub so ntswg, uas yuav ua rau muaj kev loj hlob ntawm metabolic syndrome [10]. Txiv hmab txiv ntoo thiab zaub muaj biologically active compounds xws li carotenoids, anthocyanins, thiab polyphenols, uas qhia tau hais tias yav tom ntej.antioxidantkev txiav txim thiab tseem tuaj yeem ntxiv dag zog tiv thaiv kab mob metabolic [11, 12]. Txiv nkhaus taw tev yog ib qho tseem ceeb ntawm cov txiv hmab txiv ntoo uas suav tias tsis tuaj yeem noj, nplua nuj nyob hauv flavonoids, polyphenols, thiab carotenoids ua antioxidants. Nws yog kwv yees li 15-20 feem pua ntawm cov txiv hmab txiv ntoo hnyav thiab yog cov khoom pov tseg loj ntawm cov txiv hmab txiv ntoo ua haujlwm [13]. Mango tev hmoov tiv thaiv kab mob rog rog thiab ua rau lub raum thiab lub siab ua haujlwm tsis zoo raws li kev siv tshuaj. Kev kho mob ntawm cov kab mob ntev uas siv cov khoom ntuj muaj nyob rau hauv txiv nkhaus taw peels muab lwm txoj hauv kev zoo dua rau cov tshuaj siv niaj hnub.
Ua raws li cov txiaj ntsig kev noj qab haus huv muaj zog ntawm cov khoom siv los ntawm cov khoom no, txoj kev tshawb fawb tam sim no tau tsim los soj ntsuam nws cov txiaj ntsig kev ua haujlwm tawm tsam qhov teeb meem loj tshaj plaws hauv zej zog, piv txwv li, dyslipidemia thiab oxidative kev nyuaj siab ntsig txog.rog rog. Txawm hais tias muaj qee qhov kev tshawb fawb yav dhau los pom muaj qhia txog kev txo qis hauv oxidative kev nyuaj siab hauv nas, tsis muaj pov thawj txaus los daws cov kev ntxhov siab oxidative hauv tib neeg cov kev siv xws li kev lag luam thiab cov peev txheej ntuj.
2. Cov ntaub ntawv thiab cov txheej txheem
2.1. Kev npaj cov khoom siv raw
Mangoes (Mangifera indica) ntau yam Chaunsa tau txais los ntawm lub khw hauv zos ntawm Faisalabad, Pakistan, thiab ntxuav thiab tev. Tom qab ntawd, cov txiv hmab txiv ntoo sib cais tau ntxuav nrog dej sov kom tshem tawm cov suab thaj thiab ziab ntawm 60 ° C hauv lub cev qhuav dej (NESCO® / American Harvest). Cov peels qhuav tau hauv av siv lub grinder (Panasonic MX AC 400 Tov Khoom Grinder) thiab sieved kom tau txais cov khoom me me ntawm 500-600 μm loj; Tom qab ntawd, cov hmoov tau muab khaws cia rau hauv lub khob iav ntawm 18 ° C thiab khaws cia rau hauv qhov chaw qhuav [14].
2.2. Kev txheeb xyuas HPLC
Mango tev extract tau npaj los ntawm mango tev hmoov (MPP) raws li txoj kev ua raws li Tunchaiyaphum li al. [15]. Cov extract ntawm MPP tau npaj siv 40 feem pua methanol hauv qhov piv ntawm 1: 1, shaken maj mam rau 5 feeb, thiab tom qab ntawd ntxiv nrog 10 mL HCl (6 M). Cov tshuaj npaj tau khaws cia rau 2 teev nyob rau hauv qhov cub ntawm 50 ° C thiab lim los ntawm microfilters ntawm 0.2-0.4 microns, thiab cov txiaj ntsig tau soj ntsuam los ntawm gradient HPLC (Model LC-10; 32KARAT SOS, Shimazdu Nyiv) [16]. Cov theem ntawm lub xov tooj ntawm tes muaj xws li acetonitrile, dichloromethane, thiab methanol (qhov sib piv 60: 20: 20, raws li), thiab tus nqi ntws ntawm cov qauv (hnyav ntim 15 μL) tau teem caij ntawm 0.8 mL / min [17].
2.3. Kev Tshawb Fawb Kev Tsim Kho
Cov txheej txheem nonprobability sampling (txoj kev yooj yim sampling) tau siv los xaiv tus qauv. Cov neeg koom tau raug xaiv los ntawm Allied Hospital thiab Civil Hospital Faisalabad, Pakistan. Cov txheej txheem suav nrog suav nrog cov poj niam uas muaj hnub nyoog 25 txog 45 xyoo uas muaj BMI ntawm 25.0-29.9 (hnyav), thaum cov neeg koom nrog muaj lwm yam teeb meem xws li ntshav qab zib, ntshav siab, plawv plawv, thiab kab mob siab tsis suav nrog. . Tawm ntawm tag nrho ntawm 120 tus neeg tuaj yeem pab dawb nyob rau theem pib, 77 tau raug tshem tawm vim muaj ntshav qab zib thiab kub siab, thiab 12 tau raug mob los ntawm qee yam mob plawv. Tag nrho ntawm 31 tus neeg rog rog tau muab faib ua ob pawg: ib qho kev kho mob (21 yam kev kawm, xaiv cov koob tshuaj ntawm txiv nkhaus taw peels hmoov) thiab lwm yam raws li kev tswj hwm (10 yam), uas tsis tau txais kev kho mob tab sis kev noj zaub mov tau muab rau ob pawg. Txhua tus neeg tuaj koom tau thov kom ua tiav daim ntawv nug txog kev pom zoo, kev kuaj lub cev, keeb kwm kev kho mob, kev ntsuas anthropometric, thiab daim ntawv nug txog zaub mov [18]. Cov koob tshuaj tau ua kom zoo thiab xaiv rau txhua qhov kev sim thiab tau muab rau hauv hnab (1 g MPP) uas yuav tsum tau noj ob zaug ib hnub ib nrab teev ua ntej noj mov nrog ib khob dej. Pawg tswj hwm tau qhia kom noj ib khob dej ib nrab ib teev ua ntej noj mov kom sib npaug. Kev noj zaub mov muaj rog thiab carbohydrates ntau tau txwv hauv txhua pab pawg. Txoj kev tshawb no tau txuas ntxiv rau 84 hnub tom qab uas cov ntshav coj mus kuaj dua thiab suav BMI. "Cov ntaub ntawv pom zoo tau txais los ntawm txhua tus neeg koom nrog hauv txoj kev tshawb fawb."
2.4. Kev pom zoo rau kev ncaj ncees
Txoj kev tshawb no tau pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Ncaj Ncees ntawm University rau tib neeg thiab tsiaj tshawb fawb (ERC/GCUF/1966-IRB-566), thiab tag nrho cov kev tshawb fawb tau ua raws li cov qauv kev coj ncaj ncees ntawm 1964 Helsinki Kev tshaj tawm thiab kev coj ncaj ncees ntawm WHO (2008) thiab nws cov kev hloov kho tom qab lossis cov qauv kev coj ua zoo sib xws.
2.5. Ntshav Biochemistry thiab Antioxidant Analysis
Cov ntshav venous tau ua tiav kom tau cov ntshav plasma thiab cov ntshav nyob rau hauv cov kab mob sterilized ntawm lub hauv paus thiab thaum kawg ntawm txoj kev tshawb no. Ib 4 mL ntshav tau muab tso rau hauv lub raj thiab cia sawv ntsug rau ib teev kom khov ntawm chav tsev kub. Tom qab cov ntshav txhaws, centrifugation tau ua rau 10 feeb ntawm 1000 rpm thiab cov ntshav muaj aliquots tau muab khaws cia rau hauv lub freezer ntawm -4 degree C. Rau kev sib cais ntawm cov ntshav, ntshav tau muab coj los rau hauv EDTA raj thiab khaws cia hauv chav sov. Tom qab ntawd, centrifugation tau ua nyob rau ntawm 2000 rpm rau 10 feeb thiab cov supernatant (plasma) tau sib cais thiab muab cia rau hauv lub freezer ntawm -4 degree C.
Txhawm rau ntsuas cov ntshav suav (CBC), cov ntshav tau coj mus rau hauv lub raj kuaj ntshav thiab cov ntshav tau txheeb xyuas los ntawm CBC auto hematology analyzer (NIPRO LE 1000 JAPAN). Cov ntshav qab zib tau raug tshuaj xyuas kom pom cov xwm txheej ntawm lipid profile siv cov txheej txheem enzymatic colorimetric [19, 20]. Tsis tas li ntawd, lub serum tau tshawb xyuas rauantioxidantraws li txoj cai siv thiobarbituric acid reactive tshuaj (TBARS) los ntawm txoj kev [21].
2.6. Kev ntsuas kev nyab xeeb
Rau lub hom phiaj kev nyab xeeb, daim ntawv nug tsis txaus siab tau sau los ntawm cov neeg koom los qhia txog kev cuam tshuam tom qab noj MPP extract. Tsis tas li ntawd, txhawm rau ua lub siab ua haujlwm kuaj (LFT) thiab lub raum ua haujlwm kuaj (RFT), cov khoom siv colorimetric raws tu qauv [22] tau txais yuav. Cov concentrations ntawm bilirubin, urea, creatinine, thiab lwm yam tau txiav txim los ntawm txoj kev [23, 24].
2.7. Kev txheeb cais
Kev soj ntsuam ntawm qhov sib txawv (ANOVA) tau siv rau kev txheeb xyuas cov ntaub ntawv [25] kom paub qhov sib txawv tseem ceeb. Qhov sib txawv tsawg kawg nkaus (LSD) tau suav los nrhiav qhov sib txawv ntawm txhais tau tias siv SPSS (Version 17, USA). Cov txiaj ntsig tau tshaj tawm tias tseem ceeb ntawm P Tsawg dua lossis sib npaug rau 0:05.
3. Cov txiaj ntsig thiab kev sib tham
3.1. HPLC Profileing
Txoj kev gradient HPLC tau siv rau kev txheeb xyuas cov tshuaj polyphenolic thiab antioxidants hauv cov txiv nkhaus taw tev extract. Cov txiaj ntsig ntawm bioactive compounds pom qhov sib txawv tseem ceeb (P Tsawg dua lossis sib npaug rau 0:05) ntawm cov tsis sib txawv (Table 1). Cov txiv nkhaus taw tev tau pom tias muaj caffeic acid ntau tshaj plaws thaum quercetin tau pom tias qis tshaj. Nws kuj tau pom tias txiv nkhaus taw tev extract muaj cov txiaj ntsig zoo ntawm cov vitamin C (49.52 ppm) nrog rau cov nyiaj tseem ceeb ntawm kaempferol, chlorogenic acid, thiab gallic acid.
HPLC chromatogram (Daim duab 1) tau pom qhov siab tshaj plaws rau lub sijhawm khaws cia uas tau muab piv nrog cov qauv paub los txheeb xyuas cov tsis paub phenolic tebchaw. HPLC kev tshuaj xyuas tau qhia tias cov tshuaj methanolic extract ntawm mango tev tau muab cov ntsiab lus siab dua ntawm cov tshuaj phenolic uas yog raws li lwm cov kev tshawb fawb [26, 27]. Lub xub ntiag ntawm cov tshuaj bioactive tseem ceeb, xws li cafes acid˃kaempherol˃gallic acid, thiab lwm yam hauv cov txiv nkhaus taw (Table 1) tuaj yeem tsis tsuas yog txo qis lipid qib ntawm plasma, ua rau inhibition ntawm lipoprotein secretion, thiab ua rau tshem tawm cov cholesterol ntxiv los ntawm cov ntshav los ntawm cov kua tsib acids tab sis kuj muaj lub luag haujlwm hauvrog rogtxo qis [28]. Cov txiv hmab txiv ntoo daj xws li txiv kab ntxwv, txiv qaub, thiab txiv nkhaus taw muaj ntau dua hauv flavonoids, polyphenols, terpenes, thiab carotenoids, uas ua raws li cov antioxidants kom tshem tawm cov dawb radicals thiab reactive oxygen hom [29, 30]. Hauv kev tshawb nrhiav no,antioxidantconcentration nyob rau hauv cov txiv nkhaus taw tev extract tau pom tias siab dua qhov kev tshawb fawb los ntawm Carvalho li al. [31]. Qhov sib txawv yuav yog vim huab cua sib txawv, cov txiv nkhaus taw cultivars, thiab kev sau qoob loo.
3.2. Cov ntaub ntawv anthropometric
Tag nrho ntawm 31 tus poj niam rog rog tuaj yeem pab dawb (10 tswj thiab 21 cov kev kho mob) nyob nruab nrab ntawm cov hnub nyoog 25 thiab 45 uas muaj ze li ntawm BMIs (Table 2). Cov txiaj ntsig tau pom tias tus nqi BMI poob qis tsis tseem ceeb nrog lub sijhawm (0th -84th hnub) hauv ob pawg. Txawm li cas los xij, qhov kev txo qis no tau tshaj tawm (los ntawm 29 mus rau 28.4 kg / m2) nyob rau hauv cov ntaub ntawv ntawm cov neeg hauv ib pab neeg kho nrog txiv nkhaus taw tev extract (Daim duab 2).
Mango tev extract txo qhov hnyav nce hauv pab pawg kho mob piv rau pawg tswj hwm. Cov kev tshawb fawb yav dhau los tau ua pov thawj tias vim kev noj cov txiv nkhaus taw, lub cev hnyav zuj zus, thiab covantioxidantqib nce [32]. Ntxiv mus, qhov kev txo qis hauv BMI tsis tseem ceeb rau kev kho mob piv rau cov pab pawg tswj thaum kawg ntawm lub sijhawm sim yuav raug ntaus nqi los ntawm kev sib xyaw ua ke ntawm bioactive compounds lub luag haujlwm rau kev tswj qhov hnyav nrog rau caffein acid thiab kaempferol [33, 34].
3.3. Kev Tshawb Fawb Txog Kev Ua Haujlwm
Tag nrho cov kev kawm tau raug tshuaj xyuas rau cov ntshav biochemistry. Cov txiaj ntsig tau pom tias hemoglobin, eosinophils, ESR (erythrocyte sedimentation rate), monocytes, polymorphs, thiab TLC (tag nrho cov leukocyte suav) tsis hloov pauv ntau (P Tsawg dua lossis sib npaug rau 0:05) ntawm kev tswj thiab kev kho mob. pab pawg, thaum cov lymphocytes tau pom tias yuav nce ntxiv hauv MPP-kho pab pawg piv rau pawg tswj hwm (Table 3).
Ntxiv mus, cov ntaub ntawv tau pom qhov sib txawv tseem ceeb (P Tsawg dua lossis sib npaug ntawm 0}: 05) rau kev kuaj lipid profile ntawm cov tswj thiab kev kho mob ntawm cov neeg rog rog (Table 3). Tom qab noj MPP, qhov txo qis hauv triglyceride (-4.63%), TC (-13.12%), thiab LDL (-9.04%), thaum 9.97 feem pua nce qib HDL hauv pab pawg kho mob piv rau pawg tswj hwm tau pom.
Muaj qhov sib txawv tseem ceeb (P Tsawg dua lossis sib npaug rau {{0}}}:05) ntawm cov pab pawg rau thiobarbituric acid reactive tshuaj (TBARS) qhov tseem ceeb (Daim duab 3). Cov tshuaj tiv thaiv kab mob antioxidant hauv pab pawg kho mob tau nce ntxiv tom qab noj txiv nkhaus taw tev hmoov (MPP) vim qhov txo qis ntawm TBARS tus nqi nrog ntau hnub kho (0th-84th).
Cov ntaub ntawv biochemical pom tau tias cov lymphocytes tau nce ntxiv hauv cov ntsiab lus thaum suav lwm cov qe ntshav tau txo qis tom qab kev cuam tshuam. Hauv kev tshuaj xyuas los ntawm Moler thiab Soft [35], nws tau piav qhia tias cov tshuaj antioxidants cuam tshuam rau cov ntshav cell suav nyob rau lub sijhawm ntev dua; yog li ntawd, nyob rau lub sij hawm luv luv raws li nyob rau hauv cov ntaub ntawv ntawm qhov kev tshawb fawb tam sim no, cov txiaj ntsig tsis tau hais ntau.
Tsis tas li ntawd, cov txiaj ntsig tau pom tias kev tswj hwm ntawm dyslipidemia nrog txo cov ntshav plasma triglyceride thiab qib roj cholesterol hauv cov neeg rog rog tau kho nrog txiv nkhaus taw tev hmoov. Yog li, nws pom tias muaj kev tiv thaiv kab mob vascular puas tsuaj los ntawm oxidation ntawm LDL. Cov kev tshawb pom no yog ua raws li qhov pom los ntawm Duttaroy thiab Jørgensen [36]. Qhov txo qis hauv cov concentration ntawm LDL cov roj cholesterol tuaj yeem raug lees paub rau bioactive compounds nrog ntau dua antioxidant muaj peev xwm muaj nyob rau hauv txiv nkhaus taw peels. Lawv ua rau txo qis ntawm cov protein uas koom nrog hauv lipogenesis; Yog li ntawd, lipogenesis yog repressed, whereas siv zog yog txhim khu kev qha muab ib tug zoo correlation rau lub adiposity. Hauv txoj kev tshawb fawb tam sim no, HDL concentration tau nce ntxiv hauv pab pawg kho mob siv MPP, uas yog ib qho tseem ceeb hauv kev thauj cov roj cholesterol ntxiv los ntawm cov hlwb thiab cov ntaub so ntswg mus rau daim siab rau qhov kawg degradation. Cov kev tshawb pom no yog ua raws li kev tshawb fawb ua haujlwm ntawm Muruganandan li al. [37], uas kuj pom tias muaj kev nce qib ntawm HDL hauv cov nas mob ntshav qab zib. Nrog kev kho mob ntawm MPP, cov tshuaj tiv thaiv antioxidant tau txhim kho hauv cov kev kawm thiab TBARS qhov tseem ceeb tau txo qis. TBARS yog cov khoom lag luam ntawm lipid peroxidation thiab suav nrog aldehydes thiab lipid hydroperoxides uas tau txhim kho thaum lub sij hawm oxidative kev nyuaj siab. Nws piav qhia txog lipid peroxidation nyob rau hauv cov nqe lus ntawm MDA thiab yog qhov qhia txog kev tiv thaiv antioxidant. Yog li ntawd, kev noj txiv nkhaus taw tev hmoov tsis tsuas yog tswj cov qib lipid tab sis kuj tseem muaj txiaj ntsig tiv thaiv lipid peroxidation [38].
3.4. Kev ntsuas kev nyab xeeb
Ob pawg kuj tau raug soj ntsuam rau kev ntsuam xyuas kev nyab xeeb nrog rau daim ntawv nug tsis xis nyob kom paub tias muaj kev cuam tshuam tsis zoo rau cov kev kawm. Daim ntawv nug tsis xis nyob tsis pom muaj kev cuam tshuam loj heev tsuas yog txo qis qab los noj mov hauv pab pawg kho mob piv rau pawg tswj hwm. Rau kev kuaj lub siab ua haujlwm (LFT) thiab lub raum ua haujlwm (RFT), ntshav bilirubin, alkaline phosphate (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), ntshav urea, thiab creatinine, raws li, pom qhov sib txawv ntawm P Less tshaj lossis sib npaug rau 0:05 (Table 4).
Bilirubin concentration yog ib qho kev qhia ntawm daim siab thiab cov kua tsib. Tsis tas li ntawd, bilirubin ua rau txo qis cov roj cholesterol hauv cov ntshav los ntawm kev nce qib HDL thiab txo qis LDL. Txoj kev tshawb fawb tam sim no tau pom tias muaj kev nce siab ntawm bilirubin nyob rau hauv pab pawg kho mob uas tej zaum yuav yog vim muaj cov tshuaj tiv thaiv kab mob antioxidant ntxiv ntawm tus neeg mob thiab txo qhov mob [39]. Tsis tas li ntawd, kev noj MPP txo alkaline phosphatase (ALP) concentration thiab yuav tsum muaj kev cuam tshuam rau adipocytes thiab fatty deposits. ALP isozyme tau tshaj tawm tias tau hais tawm hauv adipocytes uas thaum kawg nce cov roj deposits thaum lub sij hawm txhim kho adipogenesis hauvrog rog[40]. Raws li qib ALP yog qhov qhia txog kev rog rog yog li kev txo qis ntawm ALP qib los ntawm MPP tau pom tias muaj kev nyab xeeb ntawm mango tev koob tshuaj kom txo cov rog rog. Cov kev hloov pauv hauv daim siab thiab lub plawv ua rau muaj kev nce ntxiv ntawm ALT thiab AST uas yog siv los ua biomarkers rau kev kuaj mob ntawm daim siab puas tsuaj [41]. Tsis tas li ntawd, cov kab mob uas tsis yog-coholic fatty siab (NAFLD) yog cov kab mob metabolic feem ntau cuam tshuam txog kev rog rog uas qhov nce qib ntawm cov enzymes siab ua rau mob hnyav.rog rog. ALT thiab AST raug tso tawm rau hauv cov ntshav ncig hauv cov lus teb rau cov txheej txheem cell deterioration vim qhov siab peroxidation ntawm lipids, thiab nyob rau hauv cov xwm txheej no, cov tshuaj tiv thaiv antioxidant ntawm lub cev poob ntau. Qhov kev nce hauv enzyme concentrations no yog vim cov khoom noj muaj roj ntau thaum lub sijhawm cuam tshuam yog li txiv nkhaus taw tev hmoov ua haujlwm kom txo tau cov enzyme ntau ntau [42]. Txawm li cas los xij, mango tev supplementation kuj tau tshaj tawm los txhim kho qhov concentration ntawm cov enzymes hauv kev kho mob rog vim muaj cov ntsiab lus polyphenolic ntau dua nrog lub zog muaj txiaj ntsig antioxidant [43]. Hauv kev tshawb fawb tam sim no, urea thiab creatinine tau txo qis hauv pab pawg kho mob piv rau pawg tswj hwm thiab pom tias muaj txiaj ntsig ntawm MPP tiv thaiv kev tsis zoo ntawm lub raum ua haujlwm. Creatinine yog ib yam khoom ntawm cov leeg nqaij uas thauj mus rau lub raum los ntawm lub raum glomeruli rau kev pom thiab qee qhov raug pov tseg hauv cov zis. Creatinine concentration nce nrog hnub nyoog, poj niam txiv neej, thiab BMI [44], thiab cov txiaj ntsig no yog raws li cov txiaj ntsig tau pom los ntawm Morsi li al. [45].
4. Cov lus xaus
Kev rog rogtab tom nce ntawm qhov ceeb toom uas ua rau muaj kev phom sij ntxiv vim yog kev tsim cov pa oxygen reactive (ROS). Raws li cov txiaj ntsig kev tshawb fawb tam sim no, nws tuaj yeem txiav txim siab tias bioactive compounds los ntawm kev ua liaj ua teb pov tseg (txiv nkhaus taw peels) nthuav tawm ib qho kev daws teeb meem los tawm tsam ROS uas tsim tawm hauv lub cev. Yog li, cov no yog pab tiv thaiv kev puas tsuaj tshwm sim, siv cov peev txheej kev lag luam uas txwv tsis pub mus pov tseg thiab dhau los ua cov pa phem.
Cov ntaub ntawv muaj
Txhua hom ntaub ntawv muaj nyob rau hauv phau ntawv tshawb fawb. Kev tsis sib haum xeeb ntawm kev txaus siab Cov neeg sau ntawv tshaj tawm tsis muaj qhov tsis sib haum xeeb ntawm kev txaus siab.
Kev lees paub Cov kws sau ntawv ua tsaug rau cov neeg koom tes (Volunteers) thiab Tsev Kho Mob ntawm Faisalabad, rau lawv txoj kev koom tes zoo. Cov kws sau ntawv ua tsaug rau Tsoom Fwv Tsev Kawm Qib Siab University, Faisalabad, rau kev muab nyiaj txiag thiab kev tshawb fawb. Cov kws sau ntawv nthuav tawm lawv txoj kev txaus siab rau cov kws tshawb fawb txhawb nqa tus lej (RSP-2020/293) King Saud University, Riyadh, Saudi Arabia.
Farkhanda Arshad, 1 Huma Umbreen, 2 Iqra Aslam, 3 Arruje Hameed, 1 Kiran Aftab, 4 Wahidah H. Al-Qahtani, 5 Nighat Aslam, 6 thiab Razia Noreen 11 Department of Biochemistry, Government College University, Faisalabad-, Pakistan
2 Department of Nutrition Sciences, Government College University, Faisalabad-, Pakistan
3 Department of Biochemistry, University of Management thiab Technology, Sialkot Campus, Sialkot-, Pakistan
4 Department of Chemistry, Government College University, Faisalabad-, Pakistan
5 Department of Food Science thiab Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia
6 Department of Biochemistry, Independent Medical College, Faisalabad-, Pakistan
Cov ntawv xov xwm yuav tsum tau hais rau Razia Noreen; Tau txais 18 Tsib Hlis 2021; Tau txais 29 Cuaj hlis 2021; Luam tawm Lub Kaum Hli 23, 2021
Academic Editor: Andrei Surguchov
Copyright © 2021 Farkhanda Arshad li al. Qhov no yog ib tsab xov xwm qhib nkag tau muab faib raws li Creative Commons Attribution License, uas tso cai rau kev siv tsis txwv, kev faib tawm, thiab luam tawm hauv ib qho nruab nrab, yog tias cov haujlwm qub raug suav hais tias yog.
Cov ntaub ntawv
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