Kev Kho Mob Zoo Thiab Mechanism Ntawm Ligusticum Wallichii Extraction Thiab Cov Tshuaj Ntawm Testosterone- Induced Hair Loss hauv C57BL/6 nas Ⅱ

3 Cov txiaj ntsig thiab kev sib tham

3.1 Cov plaub hau loj hlob hauv nas

Cov plaub hau loj hlob hauv nas muaj telogen, catagen thiab anagen theem, hu ua lub voj voog anagen, thiab cov xim ntawm daim tawv nqaij depilated tob zuj zus raws li cov hauv paus plaub hau hloov ntawm telogen mus rau anagen. Cov hauv paus plaub hau ntawm C57BL / 6 nas muaj melanocytes, uas tuaj yeem ua ke ntau ntawm melanin thaum lub sij hawm loj hlob, thiab daim tawv nqaij yog xim dub; lub synthesis ntawm melanin txo nyob rau hauv lub regression theem, thiab daim tawv nqaij yog grey; Cov hauv paus plaub hau ntawm 6-8 lub lim tiam qub C57BL/6 nas nkag mus rau theem telogen, yog li ntawd, tus qauv ntawm androgenetic alopecia tuaj yeem tsim tau yooj yim los ntawm kev tswj hwm.testosteronelub sijhawm no.

Nws tuaj yeem pom los ntawm daim duab 1 tias Hnub 1 yog cov tawv nqaij rov qab ntawm cov nas tam sim tom qab qhov kawg ntawm kev ua qauv 1 hnub, cov xim yog grey thiab du, thiab cov plaub hau loj hlob nyob rau hauv lub xeev ntawm so. Tom qab kev txhawb nqa nrog cov tshuaj suav tshuaj sib txawv, nws tuaj yeem ua rau cov plaub hau loj hlob mus rau theem kev loj hlob. Hnub 4 ntawm kev tswj hwm, sab nraub qaum ntawm cov nas hauv txhua pawg tswj hwm yog

Cov plaub hau saum toj kawg nkaus tsis loj hlob, tab sis daim tawv nqaij maj mam darkened. Tom qab 8 hnub ntawm kev tswj hwm, cov plaub hau kev loj hlob zoo li pom tseeb, thiab tsuas yog ob peb thaj chaw ntawm cov nas hauv cov qauv pab pawg tau loj hlob cov plaub hau, thaum cov nas hauv pawg tswj hwm muaj ntau qib ntawm cov plaub hau kev loj hlob, thiab kev nce qib ntawm cov nyhuv qis. -dose chuanxiong pawg tau muab piv nrog rau lwm pab pawg. Cov tshuaj pab pawg tau pom tseeb dua, qhia tias minoxidil, koob tshuaj siab thiab qis ntawm chuanxiong thiab cov khoom sib xyaw tuaj yeem ua rau lub sijhawm hloov pauv ntawm cov hauv paus plaub hau los ntawm telogen mus rau anagen thiab lub sijhawm los ntawm kev nkag mus rau anagen rau cov plaub hau loj hlob ntawm epidermis. Tom qab 12 hnub, tsuas yog ib qho me me ntawm cov plaub hau loj hlob nyob rau hauv cov nas nyob rau hauv cov qauv pab pawg neeg, feem ntau ntawm sab nraub qaum yog tseem liab qab, daim tawv nqaij dawb, thiab cov plaub hau loj hlob nyob rau hauv lub telogen theem. Txawm li cas los xij, cov plaub hau nyob rau sab nraub qaum ntawm cov nas hauv txhua pawg thawj coj tau npog tag nrho thaj chaw depilation ntawm sab nraub qaum, thiab xim yog grey-dub thiab ci iab. Qee cov nas tau tsim cov plaub hau grey rau ntawm lawv nraub qaum, thiab qee cov nas tau kho nrog cov tshuaj Chuanxiong extract tau pom cov plaub hau poob ntawm lub caj dab thiab lub pob tw.

3.2 Kev txiav txim siab ntawm cov plaub hau ntev thiab cov plaub hau npog

It can be seen from Table 1 that on the 8th and 10th days of treatment, the mice in the model group had no back hair or the length could not be measured, while the treatment group grew shorter hairs (3 and 5 mm). On the 12th day, different degrees of new hair grew on the backs of mice in all groups, and the ratio of the length of new hair in each administration group to the length of hair in the model group (EE1) was used to evaluate the effect of each formula on promoting hair recovery. The order of promotion effect: low-dose Chuanxiong group>minoxidil group>compound formula group>Chuanxiong high-dose pawg. Ntawm lawv, EE1 ntawm pawg Chuanxiong qis qis mus txog 3.4, tom qab ntawd los ntawm minoxidil thiab Angelica cov tshuaj qis qis, EE1 mus txog 3.2. Cov txiaj ntsig tau pom tias Chuanxiong extract thiab cov tshuaj sib xyaw ua ke muaj txiaj ntsig zoo rau kev txhawb cov plaub hau kev loj hlob, thiab pawg Chuanxiong qis tau muaj txiaj ntsig zoo dua li pawg ntau dua, qhia tias cov txiaj ntsig ntawm Suav tshuaj ntsuab rho tawm ntawm kev txhawb cov plaub hau kev loj hlob. mus rau lub concentration.

Nws tuaj yeem pom los ntawm Table 2 tias nyob rau hnub 4 ntawm kev kho mob, tsis muaj plaub hau loj hlob hauv thaj chaw alopecia ntawm cov nas hauv txhua pawg. Hnub 8, ib feem me me ntawm sab nraub qaum ntawm cov nas hauv pawg qauv tau npog nrog cov plaub hau, thaum ntau tshaj li ib nrab ntawm cov nas tom qab hauv pawg thawj coj tau npog cov plaub hau. Hnub tim 12, txhua tus nas tau npog lawv nraub qaum mus rau qhov sib txawv.

Cover the hair, calculate the percentage of hair coverage (%) based on the ratio of the newly-born hair coverage area of the mice in each group to the area of the total shaved area, and use the ratio of the hair coverage percentage of the mice in each administration group and the hair coverage percentage of the model group on the 12th day. The ratio (EE2) was used to evaluate the promoting effect of each formula on hair recovery. The order of promotion effect: low-dose Chuanxiong group> compound formula group> minoxidil group>Chuanxiong high-dose pawg. Ntawm lawv, EE2 ntawm Chuanxiong cov tshuaj qis qis tau mus txog 5.4, ua raws li pawg formula compound, uas mus txog 5.3.

Cistanche rau kev txhim kho testosterone

3.3 Kev txiav txim siab ntawm daim tawv nqaij noo noo, roj thiab kub

Txo cov tawv nqaij noo noo, roj ntau ntxiv, thiab kub siab yog qhov tseem ceeb ntawm AGA [3].

. Nws tuaj yeem pom los ntawm daim duab 2 uas piv nrog cov pab pawg dawb huv, qhov dej noo ntawm cov tawv nqaij rov qab ntawm cov nas hauv pawg qauv poob qis (P < 0.05),="" cov="" roj="" cov="" ntsiab="" lus="" tau="" nce.="" (p=""><0.05), thiab="" kub="" kuj="" nce.="" tus="" qauv="" tsim="" ua="" tiav.="" tom="" qab="" raug="" kho="" nrog="" cov="" qauv="" sib="" txawv,="" cov="" dej,="" roj="" thiab="" qhov="" kub="" thiab="" txias="" ntawm="" cov="" nas="" hauv="" pawg="" tswj="" hwm="" tau="" rov="" qab="" mus="" rau="" qhov="" sib="" txawv.="" rau="" cov="" dej="" noo,="" cov="" tawv="" nqaij="" noo="" noo="" tus="" nqi="" ntawm="" cov="" nas="" nyob="" rau="" hauv="" txhua="" pawg="" thawj="" coj="" rov="" qab="" mus="" rau="" theem="" ntawm="" pab="" pawg="" neeg="" dawb="" paug,="" thiab="" cov="" pab="" pawg="" neeg="" ligusticum="" chuanxiong="" muaj="" cov="" txiaj="" ntsig="" zoo="" tshaj="" plaws.="" cov="" pab="" pawg="" ua="" ke="" tau="" pom="" tseeb="" dua="" li="" pawg="" minoxidil;="" qhov="" kub="" ntawm="" daim="" tawv="" nqaij="" nyob="" rau="" hauv="" txhua="" pab="" pawg="" neeg="" tswj="" hwm="" rov="" qab="" mus="" rau="" qhov="" qub.="" cov="" txiaj="" ntsig="" tau="" pom="" tias="" ob="" qho="" tib="" si="" chuanxiong="" extract="" thiab="" cov="" compound="" muaj="" qhov="" cuam="" tshuam="" ntawm="" kev="" thim="" rov="" qab="" ntawm="" daim="" tawv="" nqaij="" noo="" noo="" cov="" ntsiab="" lus,="" ntau="" cov="" roj="" tso="" tawm="" thiab="" rov="" ua="" kom="" qhov="" kub="" thiab="" txias="" ntawm="" daim="" tawv="" nqaij,="" yog="" li="" tiv="" thaiv="" cov="" plaub="" hau="" poob="" thiab="" txhawb="" cov="" plaub="" hau="" kev="" loj="" hlob.="" dil="" ua="" haujlwm="" zoo="">

3.4 Kev soj ntsuam ntawm cov kab mob pathological

Cov txiaj ntsig ntawm kev tshuaj xyuas histopathological (Daim duab 3) tau pom tias cov hauv paus plaub hau ntawm daim tawv nqaij ntawm cov nas nyob rau hauv pawg neeg dawb huv yog ntau dua, thiab qee qhov ntawm lawv nyob rau theem kev loj hlob. Piv nrog rau cov pab pawg neeg dawb paug, qhov ntev ntawm daim tawv nqaij ntawm cov nas nyob rau hauv cov qauv pab pawg pom tau tias shrinkage thiab atrophy ntawm cov hauv paus plaub hau, nrog los ntawm ib tug txo nyob rau hauv tag nrho cov thickness ntawm daim tawv nqaij, thiab morphology ntawm cov plaub hau follicles qhia tau hais tias lawv yog. tseem nyob rau hauv lub sijhawm so, qhia tias tus qauv AGA tau ua tiav hauv C57BL / 6 nas. Tsim kom muaj. Tus qauv AGA yog tus cwj pwm los ntawm kev maj mam me me ntawm cov hauv paus plaub hau. Cov hauv paus plaub hau me me nyob hauv theem telogen thiab tsis tuaj yeem nkag mus rau theem kev loj hlob ntawm lub voj voog tom ntej. Nws kuj tseem cuam tshuam txog kev txo qis ntawm cov plaub hau txoj kab uas hla, uas yuav ua rau cov plaub hau davhlau ya nyob twg los ua vellus plaub hau [6]. Piv nrog rau pawg qauv, cov tawv nqaij tuab ntawm cov nas nyob rau hauv txhua pawg tswj hwm tau zoo rov qab mus rau theem ntawm pawg dawb huv. Tom qab kev cuam tshuam nrog ntau yam tshuaj ntsuab hauv Suav teb, tus naj npawb thiab txoj kab uas hla ntawm cov hauv paus plaub hau hauv nas tau nce ntxiv. Ntawm lawv, cov nas nyob rau hauv pawg neeg Chuanxiong siab muaj ntau tshaj plaws ntawm cov plaub hau follicles hyperplasia thiab qhov ntim ntawm cov hauv paus plaub hau kuj loj, uas yog piv rau cov pab pawg minoxidil, uas pab kom cov plaub hau loj hlob thiab tsim cov plaub hau tuab. cov ncej. Ntxiv nrog rau cov plaub hau follicle morphology, piv nrog cov qauv pab pawg, qhov tseem ceeb ntawm melanin nce hauv txhua pawg tswj hwm kuj tuaj yeem qhia tau tias cov hauv paus plaub hau tau nkag mus rau theem kev loj hlob.

3.5 Kev Tshawb Nrhiav VEGF, -MSN thiab TGF- 1 cov ntsiab lus hauv cov tawv nqaij nas Hauv qhov kev sim no, kev qhia ntawm VEGF, -MSN thiab TGF- 1 tau siv los ntsuas cov txiaj ntsig kho mob ntawm cov tshuaj suav tshuaj suav tshuaj ntawm cov plaub hau. poob, thiab sim piav qhia Chuanxiong los ntawm qib molecular. Lub mechanism ntawm anti-poob nyhuv ntawmtshuaj suav tshuaj. Cov plaub hau txuas ntxiv muaj kev hloov pauv hauv cov plaub hau follicle morphology thiab nws cov microvascularization, thiab alopecia cuam tshuam nrog txo cov plaub hau follicle microvascularization. VEGF yog ib qho ntawm ntau yam angiogenic, muaj zog pro-angiogenic kev ua, tuaj yeem txhawb kev tsim cov microvessels hauv cov txheej txheem ntawm cov plaub hau follicle sib txawv, yog li nws tseem ceeb heev rau cov plaub hau follicle plaub hau txuas ntxiv [14]. Rau kev qhia ntawm VEGF (vascular endothelial txoj kev loj hlob zoo tshaj plaws) (Daim duab 4), tom qab kev kho mob nrog cov tshuaj sib txawv, qib ntawm VEGF nyob rau hauv daim tawv nqaij ntawm nas tau nce ntau dua piv nrog cov qauv pab pawg, ntawm cov pab pawg tsawg ntawm Chuanxiong. muaj qhov pom tseeb tshaj plaws, ua raws li qhov siab ntawm Chuanxiong. pab pawg thiab minoxidil pawg, thaum cov tshuaj sib xyaw ua ke muaj cov txiaj ntsig tsis tshua pom tseeb. -MSH thiab TGF- 1 yog zais los ntawm anagen plaub hau qhov muag teev, uas yog qhov tseem ceeb rau kev saib xyuas cov plaub hau follicle tiv thaiv kev zam txim [15]. Lub cev tsis muaj zog ntawm cov hauv paus plaub hau yog ib qho dynamic, periodic, thiab txheeb ze amnesty xeev, uas tsuas yog muaj nyob rau hauv lub sij hawm loj hlob ntawm cov plaub hau follicle. Qhov chaw mus tas li nkag mus rau lub sijhawm so [16]

Yog li ntawd, lub restoration thiabkev saib xyuas kev tiv thaiv kev zam tximtxiav txim qhov pib thiab lub sij hawm ntawm kev loj hlob theem ntawm cov plaub hau follicle thiab yogQhov tseem ceeb rau kev tswj cov plaub hau kev loj hlob. As shown in Figure 5, compared with the model group, the content of α-MSN in the skin of mice in different administration groups was increased, and the content levels were in descending order: Chuanxiong low-dose group > compound formula group > > positive group > Chuanxiong high-dose group. For the expression of TGF-β1 (Fig. 6), the order of content level from high to low was: compound formula group> Chuanxiong low-dose group> Minoxidil group>Chuanxiong high-dose pawg. Zuag qhia tag nrho, cov plaub hau kev loj hlob-txhim kho cov nyhuv ntawm Chuanxiong extract thiab complex formulation tej zaum yuav muaj feem xyuam rau nws enhanced restoration thiab txij nkawm ntawm cov plaub hau follicle tiv thaiv amnesty thiab angiogenesis nyob rau hauv alopecia cheeb tsam.