Lub Ntiaj Teb Thawj autologous Regenerated Islet Transplantation tau ua tiav, Thiab Tus Neeg Mob Nrog A 25-xyoo keeb kwm ntawm ntshav qab zib tau kho

Thaum Lub Plaub Hlis 30, 2024, Xib Fwb Yin Hao pab neeg los ntawm Lub Tsev Kho Mob Thib Ob ntawm Naval Medical University (Shanghai Changzheng Tsev Kho Mob) thiab Xib Fwb Cheng Xin pab neeg los ntawm Center for Excellence hauv Molecular Cell Science, Suav Academy ntawm Kev Tshawb Fawb tau luam tawm ib tsab xov xwm nyob rau hauv lub ntiaj teb no renowned. Phau ntawv Journal Cell Discovery. IF: 33.5) luam tawm cov ntaub ntawv tshawb fawb hauv online hu ua "Kev kho tus neeg mob ntshav qab zib hom 2 nrog kev ua haujlwm tsis zoo ntawm pancreatic islet muaj nuj nqi los ntawm tus kheej endoderm qia cell-derived islet cov ntaub so ntswg": thawj daim ntawv tshaj tawm thoob ntiaj teb ntawm autologous regenerative islet transplantation siv cov qia cell, kev kho mob zoo. ntshav qab zib mellitus nrog kev ua haujlwm tsis zoo ntawm pancreatic islet. Cov thev naus laus zis no tsis yog tsuas yog coj cov kev hloov pauv tshiab uas tsis tau muaj dua los rau hauv kev kho mob ntshav qab zib tab sis kuj ua rau muaj kev cia siab tshiab rau ntau pua lab tus neeg mob ntshav qab zib thoob ntiaj teb.

Nyem rau Cistanche rau mob raum

Kev ua txhaum loj hauv kev kho mob ntshav qab zib regenerative!

Ntshav qab zib mellitus, raws li ib qho kev mob hnyav zuj zus, ua rau muaj kev hem thawj loj rau tib neeg kev noj qab haus huv. Ntawm lawv, hom 2 mob ntshav qab zib mellitus (T2D) feem ntau pib nrog insulin tsis kam nyob rau hauv cov ntaub so ntswg peripheral, thiab maj mam poob pancreatic islet muaj nuj nqi raws li tus naj npawb ntawm cov hlwb txo qis los yog cov hlwb dedifferentiate, thiab ntau dua 30% ntawm cov neeg mob T2D thaum kawg vam khom exogenous insulin. Yog tias cov neeg mob muaj kev tswj tsis tau cov ntshav qab zib mus ntev, lawv yuav raug kev txom nyem loj xws li dig muag, lub raum tsis ua haujlwm, mob plawv thiab mob hlab ntsha tawg, thiab kev txiav tawm. Qhov hnyav dua, cov teeb meem hnyav xws li ketoacidosis lossis tsis txhob txwm ua rau lub cev tsis muaj zog tuaj yeem ua rau tuag taus.

Tuam Tshoj muaj cov neeg mob ntshav qab zib ntau tshaj plaws hauv ntiaj teb. Tus naj npawb ntawm cov neeg mob ntshav qab zib yog siab li 140 lab, ntawm uas muaj li 40 lab tus neeg mob xav tau lub neej ntev ntawm kev txhaj tshuaj insulin rau kev kho mob. Rau cov neeg mob hnyav uas nws cov ntshav qab zib tsis yooj yim tswj tau, tsuas yog kev kho mob zoo tam sim no yog rho tawm cov ntaub so ntswg los ntawm pancreas pub dawb thiab ua cov tshuaj hloov pauv los ntawm kev phais me me kom tsis txhob muaj teeb meem ntxiv. Txawm li cas los xij, txwv los ntawm qhov tsis txaus siab ntawm cov neeg pub khoom nruab nrog cev thiab cov cuab yeej sib cais ntawm cov islet complex, qhov kev thov rau kev kho mob nyob deb tsis tau ntsib.

Yog li ntawd, yuav ua li cas rov tsim dua tib neeg islet cov ntaub so ntswg ntawm qhov loj hauv vitro tau dhau los ua qhov teeb meem ntawm kev txhawj xeeb thoob plaws ntiaj teb kev kawm thiab kev kho mob. Cov txiaj ntsig kev tshawb fawb tshiab qhia tau hais tias nrog kev siv zog ntawm peb lub teb chaws cov kws tshawb fawb tshawb fawb, cov cuab yeej no tau ua tiav qhov kev ua tiav loj!

Los ntawm "beta cell tsis ua haujlwm" mus rau "ua tiav tshem tawm ntawm insulin"

Xibfwb Yin Hao, tus thawj coj ntawm Lub Chaw Hloov Lub Cev Hauv Tsev Kho Mob ntawm Shanghai Changzheng Tsev Kho Mob, tau hais tias tom qab ntau tshaj kaum xyoo ntawm kev tshawb fawb, pab pawg tshawb fawb txog kev siv tus neeg mob cov ntshav PBMC rov ua dua rau hauv autologous iPSC hlwb, thiab siv lub ntiaj teb thawj thev naus laus zis los hloov pauv. Lawv mus rau hauv "cov noob hlwb", uas yog, endodermal qia hlwb ( Endoderm qia cell (EnSC), thaum kawg paub txog kev rov tsim dua ntawm islet cov ntaub so ntswg (E-islet) hauv vitro.

The first beneficiary of this technology is a 59-year-old male patient with a 25-year history of T2D. The patient developed end-stage diabetic nephropathy (uremia) and received a kidney transplant in June 2017. Since November 2019, the patient's blood sugar control has become increasingly difficult, with blood sugar levels of 3.66-14.60 mmol/L, mean blood sugar drift (MAGE) as high as 5.54 mmol/L, and strict time in range (Time in Tight Range, TITR, 3.9 -7.8 mM) is only 56.7%. The average number of daily hyperglycemic events (>100 mmol/L) yog 0.7/d, thiab qhov nruab nrab ntawm cov xwm txheej hypoglycemic txhua hnub (<3.9 mmol/L) was 0.3/d. Due to the almost complete failure of the pancreatic islet function, the patient needs to receive insulin injections multiple times a day and faces a great risk of serious complications of diabetes.

Thaum Lub Xya Hli 19, 2021, vim muaj kev txhawj xeeb txog hypoglycemia thiab kev txiav txim siab tias kev tswj ntshav qab zib tsis zoo tuaj yeem ua rau muaj kev cuam tshuam tsis zoo rau kev muaj sia nyob ntev ntawm lub raum pub dawb, tus neeg mob tau txais kev hloov pauv ntawm cov islet autologous hauv tsev kho mob Shanghai Changzheng. Tus neeg mob tau txiav tawm tag nrho los ntawm exogenous insulin pib txij lub lim tiam 11 tom qab kev phais. Nrog rau kev txo qis ntawm cov tshuaj hypoglycemic ntawm qhov ncauj, kev tshem tawm cov tshuaj tau ua tiav ntawm lub lis piam 48 thiab 56. Tus neeg mob tau txiav cov tshuaj insulin tas li rau 33 lub hlis. tshwj xeeb:

➤ Lub Limtiam 2:

Significant improvement in the patient's glycemic control was observed as early as 2 weeks after transplantation, with MAGE falling from 5.50 mmol/L to 3.60 mmol/L and TITR rapidly rising from 56.7% to 77.8%. Time above target range (TAR) decreased by 55% from baseline, and severe hyperglycemia (>13.9 mM) thiab hypoglycemia (<3.9 mM) events completely disappeared.

➤ Lub lis piam 4 ~ 12:

Qhov nruab nrab dynamic ntshav qabzib hloov pauv poob los ntawm 5.50 txog 2.6 mmol / L, thiab TITR nce tsis tu ncua los ntawm 81% mus rau 90%.

➤ Ntau dua lossis sib npaug rau 32 lub lis piam:

Tus neeg mob TITR tau yooj yim khaws cia ntawm 99%, thiab MAGE, tus qauv kub rau cov ntshav qabzib hloov pauv, raug txo los ntawm 5.50 mM mus rau 1.60 mM.

➤116-lub lim tiam tom qab kev phais:

Tsis muaj qhov tshwm sim ntawm hypoglycemia lossis hyperglycemia hnyav. Cov qib glycated hemoglobin (HbA1c) maj mam txo los ntawm 6.6% ntawm lub hauv paus mus rau 5.5% (lub lim tiam 85) thiab 4.6% (lub lim tiam 113).

Cov kws tshawb fawb tau hais tias tus neeg mob cov cim tseem ceeb xws li yoo mov thiab postprandial C-peptide tau txhim kho zoo dua piv nrog cov ua ntej kev phais, uas txhais tau hais tias pancreatic islet ua haujlwm tau zoo rov qab los. Tsis tas li ntawd, kev ua raws li cov cim qhia xws li lub raum ua haujlwm nyob rau hauv qhov qub, uas tseem qhia tau hais tias kev kho mob tuaj yeem zam qhov kev mob ntshav qab zib ntau ntxiv thiab cov kab mob graft tau txais txiaj ntsig zoo yam tsis muaj qog tsim los yog muaj teeb meem cuam tshuam loj heev. Zuag qhia tag nrho, qhov no yog thawj zaug thoob ntiaj teb tib neeg cov ntaub so ntswg hloov kho siv autologous islets los kho cov neeg mob T2D nrog kev ua haujlwm tsis zoo ntawm cov islet.

Tsom ntsoov rau "universal" regenerative islet cov ntaub so ntswg uas tsis xav tau kev tiv thaiv kab mob

Raws li Changzheng Tsev Kho Mob, ntxiv rau thawj kis mob ntshav qab zib hom 2 mob hnyav, pab pawg sib koom ua ke saum toj no kuj tau kho ntau yam mob ntshav qab zib hom 1. Nyob rau hauv lub neej yav tom ntej, pab neeg no yuav tsom mus rau kev tshawb fawb txog cov qia cell-derived regenerated islet cov ntaub so ntswg los tsim "universal" regenerated islet cov ntaub so ntswg uas tsis xav tau kev tiv thaiv kab mob, yog li muab kev kho tshiab rau feem ntau ntawm cov neeg mob ntshav qab zib uas vam khom rau insulin. txhaj tshuaj tau ntev.

Cistanche kho mob raum li cas?

Cistancheyog ib hom tshuaj suav tshuaj ntsuab siv rau ntau pua xyoo los kho ntau yam mob, suav nrograumkab mob. Nws yog muab los ntawm qhuav stems ntawmCistanchedeserticola, ib tsob nroj nyob rau hauv cov suab puam ntawm Tuam Tshoj thiab Mongolia. Lub ntsiab active Cheebtsam ntawm cistanche yogphenylethanoidglycosides, echinacoside cov tshuaj, thiabacteoside, uas tau pom tias muaj txiaj ntsig zoo rauraumnoj qab haus huv.

Kab mob raum, tseem hu ua kab mob raum, hais txog ib yam mob uas lub raum ua haujlwm tsis zoo. Qhov no tuaj yeem ua rau muaj cov khoom pov tseg thiab cov co toxins hauv lub cev, ua rau muaj ntau yam tsos mob thiab teeb meem. Cistanche tuaj yeem pab kho mob raum ase los ntawm ntau lub tshuab.

Ua ntej, cistanche tau pom tias muaj cov nyhuv diuretic, txhais tau tias nws tuaj yeem ua rau cov zis ntau ntxiv thiab pab tshem tawm cov khoom pov tseg ntawm lub cev. Qhov no tuaj yeem pab txo lub nra ntawm lub raum thiab tiv thaiv kev tsim cov co toxins. Los ntawm kev txhawb nqa diuresis, cistanche kuj tseem tuaj yeem pab txo qis ntshav siab, ib qho teeb meem ntawm cov kab mob raum.

Ntxiv mus, cistanche tau pom tias muaj cov teebmeem antioxidant. Kev ntxhov siab oxidative, tshwm sim los ntawm qhov tsis sib xws ntawm kev tsim cov dawb radicals thiab lub cev tiv thaiv antioxidant, ua lub luag haujlwm tseem ceeb hauv kev mob raum. ies pab neutralize dawb radicals thiab txo Oxidative kev nyuaj siab, yog li tiv thaiv lub raum los ntawm kev puas tsuaj. Cov phenylethanoid glycosides pom hauv cistanche tau tshwj xeeb hauv kev tshem tawm cov dawb radicals thiab inhibiting lipid peroxidation.

Tsis tas li ntawd, cistanche tau pom tias muaj cov nyhuv anti-inflammatory. Kev mob yog lwm yam tseem ceeb hauv kev loj hlob thiab kev loj hlob ntawm cov kab mob raum. Cistanche's anti-inflammatory zog pab txo cov zus tau tej cov pro-inflammatory cytokines thiab inhibit qhov ua kom o yuav tsum tau txoj kev, yog li alleviating o nyob rau hauv lub raum.

Tsis tas li ntawd, cistanche tau pom tias muaj cov teebmeem immunomodulatory. Hauv kab mob raum, lub cev tiv thaiv kab mob tuaj yeem ua rau tsis zoo, ua rau muaj kev mob ntau dhau thiab cov ntaub so ntswg puas. Cistanche pab tswj lub cev tiv thaiv kab mob los ntawm kev hloov kho kev tsim khoom thiab kev ua haujlwm ntawm lub cev tiv thaiv kab mob, xws li T hlwb thiab macrophages. Txoj cai tiv thaiv kab mob no pab txo qhov mob thiab tiv thaiv kev puas tsuaj ntxiv rau lub raum.

Ntxiv mus, cistanche tau pom los txhim kho lub raum kev ua haujlwm los ntawm kev txhawb nqa kev tsim kho ntawm lub raum hlab nrog cov hlwb. Lub raum tubular epithelial hlwb ua lub luag haujlwm tseem ceeb hauv kev pom thiab rov nqus cov khoom pov tseg thiab electrolytes. Hauv kab mob raum, cov hlwb no tuaj yeem raug puas tsuaj, ua rau lub raum ua haujlwm puas. Cistanche lub peev xwm los txhawb kev tsim kho ntawm cov hlwb no pab kho lub raum kom zoo thiab txhim kho lub raum tag nrho.

Ntxiv nrog rau cov kev cuam tshuam ncaj qha rau lub raum, cistanche tau pom tias muaj txiaj ntsig zoo rau lwm yam kabmob thiab lub cev hauv lub cev. Txoj hauv kev zoo rau kev noj qab haus huv no tseem ceeb tshwj xeeb hauv cov kab mob raum, vim tias tus mob feem ntau cuam tshuam rau ntau lub cev thiab lub cev. che tau pom tias muaj kev tiv thaiv rau lub siab, lub plawv, thiab cov hlab ntsha, uas feem ntau cuam tshuam los ntawm kab mob raum. Los ntawm kev txhawb nqa kev noj qab haus huv ntawm cov kabmob no, cistanche pab txhim kho lub raum tag nrho thiab tiv thaiv kev mob ntxiv.

Hauv kev xaus, cistanche yog cov tshuaj suav tshuaj ntsuab siv rau ntau pua xyoo los kho mob raum. Nws cov active Cheebtsam muaj diuretic, antioxidant, anti-inflammatory, immunomodulatory, thiab regenerative teebmeem, uas pab txhim kho lub raum ua haujlwm thiab tiv thaiv ob lub raum los ntawm kev puas tsuaj ntxiv. , cistanche muaj txiaj ntsig zoo rau lwm yam kabmob thiab lub cev, ua rau nws txoj hauv kev zoo rau kev kho mob raum.