Qhov Kev Tshawb Fawb Txog Kev Tshawb Fawb rau Daim Ntawv Thov Tshiab ntawm Cistanche
Mar 09, 2022
Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com
Sun Weidong1, Chen fei2, Sun yun2
(1. Yangzhou Tsev Kho Mob ntawm Tsob Ntoo Suav Tshuaj, Yangzhou 225009, Jiangsu; 2. Tsev Kawm Ntawv Tshuaj, Yangzhou University, Yangzhou 225001, Jiangsu)
TSEEM CEEB: Tus qauv beg nin prostatic hyperplasia (BPH) nas qauv tau tsim, thiab peb koob tshuaj ntawm acteoside tau muab rau hauv qhov ncauj rau 4 lub lis piam, los kawm txog cov txiaj ntsig ntawm acteoside distilled los ntawmCistanche tubulosantawm apoptosis thiab ultra microstructure prostate caj pas hauv nas nrog BPH. Kev hloov pauv ntawm cov qog prostate tau pom los ntawm kev sib kis ntawm electron microscopy. Cov txiaj ntsig tau pom tias Apoptosis ntawm cov nas prostate ib txwm tsis pom tseeb, muaj tsawg
tus nqi ntawm prostate apoptosis nyob rau hauv cov qauv pab pawg neeg, nyob rau hauv high-dose acteoside los ntawmCistanche tubulosakev kho mob, nas prostate apoptosis tau nce siab dua li ntawm cov qauv.
KEYWORDS:Cistanche, Cistanche tubulosa, acteoside; nas; pib prostatic hyperplasia; apoptosis; ultrastructural
Hauv cov txheej txheem ntawm benign prostate hyperplasia (BPH), cell apoptosis yog ib qho ntawm cov txheej txheem uas tam sim no nyiam. Gene coding txiav txim siab nws thiab ua rau cov hlwb los tswj cov txheej txheem cell tuag los ntawm kev teb rau cov cim tshwj xeeb.
Tom qab cov prostate loj hlob mus rau cov neeg laus, qhov kev loj hlob ntawm cov hlwb prostate yog khaws cia ntawm 1 feem pua rau 2 feem pua los ntawm qhov sib npaug ntawm qhov nruab nrab ntawm apoptosis. Thaum qhov sib npaug lawm, nws raug suav tias yog qhov ua rau BPH. Raws li ib txwm muaj, cov qib ntawm cov tshuaj estrogen thiab androgen thiab ib txwm ncig hauv prostate yog ib hom kev ntsuas tus kheej uas muaj nyob rau hauv cov txheej txheem ntawm kev loj hlob thiab apoptosis. Thaum qhov sib npaug ntawm kev loj hlob thiab apoptosis tsis muaj qhov sib npaug, nws yuav ua rau muaj kev loj hlob ntxiv thiab nthuav dav ntawm lub caj pas, uas feem ntau ua rau kev nthuav dav ntawm epithelial cell compartment [1].
Hauv qhov kev sim no, peb tau tsim cov qauv nas prostate hyperplasia los soj ntsuam thiab txheeb xyuas cov txiaj ntsig ntawm acteoside (coaction-zoo li glycoside), ib qho ntawm cov tshuaj lom neeg ntawmCistanchentawm apoptosis thiab ultrastructural hloov pauv ntawm nas prostate hlwb, txhawm rau ntsuam xyuas cov nyhuv ntawm acteoside Cov nyhuv ntawm inhibiting prostate hyperplasia muab lub hauv paus kev tshawb fawb rau kev txhim kho thiab kev siv tshiab ntawm Suav tshuaj Cistanche.
Cistancherho tawm
1 Cov khoom siv thiab cov txheej txheem
1. 1 Kev sim tsiaj
Tsib caug txiv neej SD nas hnub nyoog 6-7 lub lis piam, huv qib, hnyav 80-100 g, yog muab los ntawm Lub Chaw rau Kev Sib Piv Tshuaj ntawm Yangzhou University.
1. 2 Reagents thiab tshuaj
Testosterone propionate txhaj yog los ntawm Shanghai General Pharmaceutical Co., Ltd., qhov tshwj xeeb yog 25 mg·mL-1, thiab batch tooj yog 060708. PI dye tov yog los ntawm Yangzhou University Testing Center. Cov ntsiab lus ntawm Aktiside yog 945 g·kg-1, npaj los ntawm Department of suav tshuaj ntsuam xyuas, Tuam Tshoj Pharmaceutical University.
1. 3 Cov khoom siv sim
Philips Tecnai 12 kis tau tus mob electron microscope yog los ntawm Philips hauv Netherlands; FACSAria flow cytometer yog los ntawm BD hauv Tebchaws Meskas.
1. 4 Pab pawg thiab ua qauv
Tom qab castration raws li txoj kev ntawm cov ntaub ntawv [3], nas raug txhaj nrog 5 mg·(kg·d)-1ntawm testosterone propionate nyob rau sab nraum qab ntawm nas rau 4 lub lis piam los tsim ib tug prostate hyperplasia qauv. Cov qauv nas tau muab faib ua cov qauv ua qauv: qauv ntxiv rau pawg acteoside high-dose pawg, qauv ntxiv rau acteoside pawg nruab nrab-dose, qauv ntxiv rau acteoside pawg qis, kaum nas hauv txhua pab pawg; Lwm kaum tus nas ib txwm ua raws li pawg tswj hwm.
1. 5 Txoj kev tswj hwm
Ib lub lis piam tom qab kev ua qauv, pawg tshuaj acteoside siab, nruab nrab, thiab qis tau muab 60, 30, 15 mg·(kg·d)-1 los ntawm gavage, feem. Pawg qauv thiab pawg tswj hwm tau muab tso rau qhov sib npaug ntawm cov kua qaub ib hnub ib zaug rau plaub lub lis piam, hnyav ib zaug ib lim tiam, thiab kho cov tshuaj raws li lub cev hnyav.
1. 6 Qauv sau thiab ua tiav
1) Cov khoom siv thiab cov qauv npaj: 24 teev tom qab kev tswj hwm zaum kawg, ntsuas cov nas yoo mov thiab txi cov nas los ntawm lub ncauj tsev menyuam dislocation. Tam sim ntawd qhib lub plab hauv plab, tev tawm cov ntaub so ntswg nyob ib ncig ntawm lub prostate, tso lub prostate, thiab coj ib feem ntawm prostate rau siv nyob rau hauv ib tug dej khov da dej. Cov ntaub so ntswg homogenizer homogenized, npaj prostate ib leeg-cell ncua kev kawm ntawv, thiab stained nrog propidium iodide ib-kauj ruam txoj kev [4]; Lwm qhov ntawm cov ntaub so ntswg prostate tau kho hauv 25 g·L-1 glutaraldehyde los npaj cov ntu nyias nyias.
2) Npaj ib qho kev ncua ntawm tes: Nyob rau hauv aseptic mob, tshem tawm cov nas prostate tag nrho thiab txav mus rau hauv lub tais petri, ntxuav cov ntshav thiab cov ntshav txhaws ntawm cov ntaub so ntswg prostate nrog PBS, thiab tshem tawm cov rog nyob ib puag ncig, fiber ntau, thiab cell membranes. Los ntawm tib nas prostate, cov ntaub so ntswg tshiab prostate nrog qhov loj ntawm li 0.5 cm3tau muab thiab txiav rau hauv daim los npaj prostate cell suspension [5]. Muab cov ntaub so ntswg thaiv rau hauv lub tais petri, thiab ntxiv me me ntawm PBS; siv ophthalmological txiab txiav cov ntaub so ntswg nyob rau hauv ib tug dej khov da dej, zom cov homogenizer rau ib tug ncua kev kawm ntawv, ntxiv 10 mL PBS, pipette cov ntaub so ntswg homogenate, thiab siv 0.074 hli pore loj nylon mesh tau lim rau hauv lub xeem raj, centrifuged ntawm 1 500 r min-1rau 3 min, thiab nag lossis daus tau ntxuav peb zaug nrog PBS, txhua zaus ntawm 500 r min-1Lub sij hawm luv luv qis-ceev centrifugation tshem tawm cov khib nyiab ntawm tes, mus rau 0.037 hli pore loj nylon mesh lim kom tshem tawm cov cell clumps. Suav cov hlwb thiab kho cov cell concentration rau 2 × 109 hlwb·L-1. Stain nrog 1 mL PI staining tov, tiv thaiv lub teeb ntawm 4 degree rau 10 min.
3) Ultrathin seem npaj: Rau kev npaj cov ntaub so ntswg ultrathin, saib cov ntaub ntawv [6].
2 Cov txiaj ntsig thiab kev txheeb xyuas
Fig. 1 Apoptosis ntawm BPH nas qauv thiab induced kev txiav txim ntawm acteoside
ib. Ib pab pawg; b. Qauv pawg; c. Acteoside cov koob tshuaj ntau heev; d. Acteoside pawg tsawg-dose
2. 1 Cov nyhuv ntawm acteoside ntawm apoptosis ntawm nas prostate
Daim duab 1 qhia txog lub ncov ntawm apoptosis kuaj pom los ntawm cov cytometry ntws, uas qhia tau hais tias acteoside muaj cov nyhuv pro-apoptotic. Kev txheeb xyuas cov txiaj ntsig ntawm cell apoptosis tau pom tias cov nas cell apoptosis tau nce ntau (24.7 ± 1.85) feem pua thiab (16.1 ± 1.04) feem pua tom qab kev kho mob siab, nruab nrab, thiab qis ntawm cov koob tshuaj. acteoside. , (14.5±{16}}.68) feem pua , thiab pawg ib txwm (1.1±{{20}}.06) feem pua , pawg qauv (9.5±0.5) feem pua , pawg zoo (9.83± 0.76) Qhov sib txawv ntawm feem pua ntawm kev sib piv yog qhov tseem ceeb (P<0.01), indicating="" that="" aktidine="" can="" induce="" cell="" apoptosis,="" the="" effect="" is="" stronger="" than="" that="" of="" the="" positive="" control="" drug="" qianliekang="" (figure="">
Fig. 2 Lub xov tooj cua ntawm apoptosis hauv BPH nas qauv thiab effection ntawm acteoside
2.2 Cov nyhuv ntawm acteoside ntawm lub ultrastructure ntawm prostatic hyperplasia hlwb.
Electron microscopy soj ntsuam pom tau hais tias cov nas prostate ib txwm muaj cov nuclei tiav, pom tseeb nucleoli, thiab tsis sib xws nuclear chromatin faib; qhov ntxhib endoplasmic reticulum muaj qhov loj me, yog txheej txheem zoo, thiab tsis muaj vacuolation. Tsis muaj tus ntiv tes zoo li microvilli nyob rau saum npoo ntawm lub epithelium, tsis muaj qhov o ntawm mitochondria, thiab ob peb lub zais zis hauv kab noj hniav. Lub nuclei ntawm prostate hlwb nyob rau hauv cov qauv pawg tau deformed thiab shrunk, thiab cytoplasm yog ntau. Qhov ntxhib endoplasmic reticulum tau nthuav dav hauv cov duab zoo li cyst, thiab lub cyst tau ntim nrog cov protein ntau ntom ntom. Mitochondria tau o o me me (muaj cov vacuoles) Hauv nas kho nrog acteoside, lub nucleus tsis zoo piv rau pawg qauv. Lub nucleus yog concentrated, lub nuclear ceev tau nce, heterochromatin accumulated, lub cytoplasm muaj vacuoles, thiab ntxhib endoplasmic reticulum vacuoles raug txo. Cov pab pawg neeg qis qis qis muaj me ntsis wrinkled nuclei Shrinkage, tsis xwm yeem, nplua nuj cytoplasm, thiab txo qis cov kab mob endoplasmic reticulum (Daim duab 3).
Cov txiaj ntsig ntawm cistanche: anti-Apoptosis
3 Kev sib tham
Apoptosis yog txheej txheem ntawm cov txheej txheem ntawm kev tuag ntawm tes hauv ntau cov kab mob uas tswj kev txhim kho ntawm lub cev thiab tswj kev ruaj ntseg ntawm ib puag ncig sab hauv, thiab cov txheej txheem caj ces txiav txim siab nws.
Apoptotic hlwb muaj lawv cov yam ntxwv morphological thiab lom: nuclear chromatin shrinkage, DNA dav degradation thiab fragmentation, tag nrho-scale shrinkage ntawm cov hlwb, shrinkage ntawm cell membranes thiab txheej protrusions npog organelles (xws li mitochondria thiab ribosomes), thiab tsim cov yam ntxwv nuclear. fragments Apoptotic lub cev, apoptotic hlwb tsis tsuas yog khaws ib tug ua tiav cell membrane qauv thiab muaj nuj nqi tab sis kuj muaj ib tug tag nrho cov qauv ntawm organelles, uas txawv ntawm cell necrosis.
Lub molecular mechanism ntawm cell apoptosis tsis tau tag nrho elucidated txog tam sim no. Kev tshawb fawb [7] tau pom tias muaj ntau cov noob koom nrog hauv kev tswj cov noob ntawm cell apoptosis.
Apoptosis yog cov txheej txheem ntawm BPH tam sim no nyiam ntau dua. Nws yog txiav txim los ntawm gene coding thiab ua kom cov hlwb los tswj kev tuag ntawm tes los ntawm kev teb rau cov cim tshwj xeeb [1]. Tom qab cov prostate loj hlob mus rau cov neeg laus, qhov kev loj hlob ntawm cov hlwb prostate yog khaws cia ntawm 1 feem pua rau 2 feem pua los ntawm qhov sib npaug ntawm tus nqi apoptosis. Kev puas tsuaj ntawm qhov nyiaj tshuav no ib txwm suav tias yog qhov ua rau BPH [8]. Nyob rau hauv cov xwm txheej ib txwm muaj, qib ntawm cov tshuaj estrogen thiab androgen thiab tsis tu ncua nyob rau hauv prostate yog ib hom ntawm tus kheej-tso lub xeev uas muaj nyob rau hauv tus txheej txheem ntawm proliferation thiab apoptosis. Thaum qhov kev loj hlob thiab apoptosis tsis muaj qhov sib npaug, nws yuav ua rau cov qog loj hlob thiab nthuav ntxiv, feem ntau yog vim qhov nthuav dav ntawm cov kab mob epithelial cell compartment [9]. Qhov cuam tshuam ntawm qhov nyiaj tshuav no yuav yog vim muaj kev hloov pauv hauv androgen qib lossis ua haujlwm; Nws kuj tseem yuav yog vim muaj cov lus teb uas tsim los ntawm AR stimulation ntawm DHT lossis kev hloov pauv hauv DHT-mediated growth factor theem [2]. Nyob rau hauv txoj kev tshawb no, FCM kuaj cell apoptosis thiab pom tias apoptosis tus nqi ntawm prostate hlwb nyob rau hauv nas muab cov koob tshuaj ntau ntawm acteoside nce ntau. Electron microscopy cov txiaj ntsig tau pom tias cov nuclei ntawm prostate hlwb nyob rau hauv cov qauv pawg tau deformed, qhov ntxhib endoplasmic reticulum tau nthuav dav heev hauv cov cyst zoo li lub cyst, thiab lub cyst tau ntim nrog cov protein ntau ntom ntom, thiab pom muaj vacuolation. Lub mitochondria tau mob me me, thiab cov kev tshawb fawb ntsig txog[ 10-11 ] Daim ntawv tshaj tawm zoo ib yam.
Tom qab kev cuam tshuam ntawm acteoside, qib ntawm prostate hyperplasia hauv nas tau qis dua. Cov pab pawg neeg koob tshuaj ntau kuj tau pom cov tsos mob ntxov ntawm apoptosis: lub nucleus ntawm prostate hlwb tseem nyob ruaj khov, thiab endoplasmic reticulum vacuoles tau qis dua. Condensation ntawm nuclei, nce nuclear ceev, tsub zuj zuj ntawm heterochromatin, zoo ib yam li cov yam ntxwv ntawm prostate cov ntaub so ntswg hlwb nyob rau hauv nas tom qab castration [12-13]. Nws qhia tau hais tias acteoside los ntawmcistanchetuaj yeem ua rau lub cev apoptosis hauv nas nrog prostate hyperplasia, tswj thiab kho qhov sib npaug ntawm prostate cell apoptosis thiab proliferation, thiab ua lub luag haujlwm hauv inhibiting prostate cell proliferation. Cov txiaj ntsig ntawm txoj kev tshawb no thiab cov kev tshawb fawb yav dhau los [14] qhia tau hais tias inhibitory nyhuv ntawm acteoside, ib qho tshuaj tiv thaiv ntawmCistanche tubulosa, ntawm prostate hyperplasia tej zaum yuav cuam tshuam rau nws txoj kev nce qib ntawm cell apoptosis, txo AR thiab TGF-UR1 overexpression. Cov ncauj lus kom ntxaws txog kev ua haujlwm tseem yuav tsum tau kawm ntxiv.
Cistanche txiaj ntsig: txhim kho kev tiv thaiv
Cov ntaub ntawv:
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Edition, 2008, 29(3): 55-58.
