Kev Tshawb Fawb Pharmacological Ntawm Acteoside Ntawm Qhov Kev Ua Haujlwm Inhibited Pib Prostatic Hyperlasia hauv nas

Kev tshawb fawb pharmacological ntawm acteoside ntawm qhov kev txiav txim inhibited pib prostatic hyperplasia hauv nas

Yog xav paub ntxiv:ali.ma@wecistanche.com

TSEEM CEEB:Qhov pib prostatic hyperplasia (BPH) nas qauv tau tsim, thiab peb koob tshuaj pab pawgacteosidetau hais lus rau 4 lub lis piam, los kawm txog cov txiaj ntsig ntawm acteoside distilled los ntawm Cistanche tubulosa ntawm apoptosis thiab ultramicro qauv prostate av hauv nas nrog BPH. Kev hloov pauv ntawm cov qog prostate tau pom los ntawm kev sib kis ntawm electron microscopy. Cov txiaj ntsig tau pom tiasApoptosisntawm ib txwm nas prostate tsis pom tseeb, muaj tus nqi qis ntawm pro-xeevapoptosisnyob rau hauv cov qauv pab pawg neeg, nyob rau hauv high-dose acteoside kev kho mob, nas pro-xeevapoptosistau ntau dua li ntawm pawg qauv.Acteosidetuaj yeem txhawb nqa nas prostate proptosis, thiab ua lub luag haujlwm hauv inhibiting benign prostatic hyperplasia.

KEYWORDS:acteoside, Cistanche, pib prostatic hyperplasia

Nyem rau cistanche tubulosa ntau npaum thiab Cistanche khoom

Apoptosis thaum lub sij hawm prostatic hyperplasia (BPH) yog ib qho ntawm cov txheej txheem tam sim no, uas yog cov txheej txheem genetic encoded thiab ua rau cov hlwb los tswj kev tuag ntawm tes los ntawm kev teb rau cov cim tshwj xeeb. Tom qab cov prostate loj hlob mus rau cov neeg laus, qhov kev loj hlob ntawm prostate hlwb yog khaws cia ntawm 1 feem pua ​​- 2 feem pua ​​​​ntawm qhov sib npaug ntawm tus nqi nruab nrab ntawm apoptosis, thiab thaum qhov nyiaj tshuav no tau tawg nws yog suav tias yog qhov ua rau predisposing. ntawm BPH. Nyob rau hauv cov xwm txheej ib txwm muaj, cov poj niam thiab androgens nyob rau hauv lub caj pas prostate thiab nws ib txwm ncig yog lub xeev ntawm homeostasis uas twb muaj lawm ntawm proliferation thiab apoptosis. Thaum kev loj hlob tsis muaj qhov sib npaug nrog apoptosis, nws ua rau muaj kev loj hlob ntxiv thiab kev loj hlob ntawm cov qog, feem ntau tso cai rau kev nthuav dav ntawm epithelial intercellular compartment tshwm sim. Hauv qhov kev sim no, peb tau soj ntsuam thiab txheeb xyuas cov txiaj ntsig ntawm ib qho ntawm cov tshuaj lom neeg ntawmCistanche, acteoside(xws li phyllode), ntawm apoptosis thiab ultrastructural kev hloov pauv hauv cov nas prostate los ntawm kev tsim cov qauv nas ntawm prostate hyperplasia, los soj ntsuam cov nqi hluav taws xob ntawm acteoside hauv inhibiting prostate hyperplasia, thiab muab lub hauv paus kev tshawb fawb los tsim kev siv tshiab.Cistanche, Suav tshuaj.

1 cov khoom thiab cov txheej txheem

1.1 kuaj tsiaj

Tsib caug txiv neej SD nas hnub nyoog 6-7 lub lis piam, huv qib, hnyav 80-100 g, tau muab los ntawm lub chaw muab tshuaj sib piv ntawm Yangzhou University.

1. 2 reagents thiab tshuaj

Testosterone propionate txhaj yog tsim los ntawm Shanghai General Pharmaceutical Co., Ltd., specification 25 mg · ML - 1, lot 060708. PI dye tov yog muab los ntawm lub chaw kuaj ntawm Yangzhou University. acteoside cov ntsiab lus 945 g · kg - 1, npaj nyob rau hauv Department of tsoos suav tshuaj tsom xam, Tuam Tshoj Pharmaceutical University.

1. 3 cov cuab yeej sim

Philips TECNAI 12 kis tau tus mob electron microscope Dutch Philips; Facsaria flow cytometer tsim rau Becton Dickinson.

1. 4 pawg thiab ua qauv

Tus qauv prostate hyperplasia tau tsim los ntawm kev txhaj tshuaj subcutaneous ntawm testosterone propionate hauv qab nas rau 4 lub lis piam raws li cov ntaub ntawv txheej txheem. Cov qauv nas tau muab faib ua plaub pawg: cov qauv ua qauv, pawg qauv acteoside high-dose pawg, qauv acteoside pawg nruab nrab-dose, qauv acteoside pawg qis, nrog 10 nas nyob rau hauv txhua pab pawg; Lwm 10 tus nas ib txwm raug coj los ua pawg tswj hwm.

1. 5 txoj kev tswj hwm

Ib lub lim tiam tom qab ua qauv, cov nas nyob rau hauv qhov siab -, nruab nrab -, thiab cov pab pawg tsawg tau txais 60, 30, thiab 15 mg · (kg · d) - 1 los ntawm gavage, feem. Cov qauv thiab cov pab pawg tswj tau intragastrically tswj hwm nrog sib npaug ntim ib txwm saline, ib hnub ib zaug rau 4 lub lis piam, thiab lub cev hnyav tau hnyav ib lub lis piam los kho cov tshuaj raws li lub cev hnyav.

1. 6 tus qauv sau thiab ua tiav

1) Cov qauv thiab kev npaj: 24 teev tom qab noj mov kawg, lub cev hnyav ntawm cov nas yoo mov tau hnyav, thiab cov nas tau txi los ntawm lub ncauj tsev menyuam dislocation, thiab lub plab hauv plab tau qhib tam sim ntawd thiab cov ntaub so ntswg nyob ib puag ncig tau raug tshem tawm, thiab cov prostate dawb. raug tshem tawm, thiab qee qhov ntawm cov prostates tau homogenized nyob rau hauv cov ntaub so ntswg homogenizer nyob rau hauv ib tug dej khov da dej los npaj ib leeg-cell suspensions ntawm prostates, uas yog stained nrog propidium iodide nyob rau hauv ib tug txheej txheem ib-kauj ruam; Lwm feem ntawm cov ntaub so ntswg prostate tau tsau hauv 25 g · L - 1 glutaraldehyde, thiab cov ntu ultrathin tau npaj.

2) Kev npaj ntawm ib leeg-cell suspensions: cov nas tsis zoo prostate raug tshem tawm mus rau hauv ib lub thawv uas tsis muaj menyuam, cov ntshav thiab cov ntshav txhaws ntawm cov nqaij mos ntawm cov nqaij mos tau muab yaug nrog PBS kom tshem tawm cov rog nyob ib puag ncig thiab cov fiber ntau thiab cytoplasmic membranes, thiab cov ntaub so ntswg tshiab prostate kwv yees li ntawm 0.5 cm3 hauv qhov loj tau sau los ntawm tib cov nas prostate, minced, thiab ib qho prostate cell suspension tau npaj.

Ib qho thaiv ntawm cov ntaub so ntswg prostate tau muab tso rau hauv Petri tais thiab ib qho me me ntawm PBS tau ntxiv; Cov ntaub so ntswg raug tshem tawm nrog ophthalmic txiab nyob rau hauv ib da dej dej khov, homogenizer hauv av rau kev ncua, 10 ml PBS tau ntxiv, cov ntaub so ntswg homogenate tau aspirated los ntawm pipette, lim rau hauv cov raj nrog 0.074 mm pore loj nylon mesh, centrifuged ntawm 1500 R · min - 1 rau 3 min, thiab cov pellet tau ntxuav peb zaug ntxiv nrog PBS, txhua zaus nrog 500 R · min - 1 luv luv-speed centrifugation rau tshem tawm cov khib nyiab ntawm tes, thiab 0.037 hli pore loj nylon mesh lim kom tshem tawm cov cell clumps. Cells raug suav thiab hloov mus rau lub cell concentration ntawm 2 × 109 · L - 1. Stain nrog 1 ml PI dye tov rau 10 min ntawm 4 ° C hauv qhov tsaus ntuj.

3) Ultrathin seem npaj: ultrathin cov ntaub so ntswg npaj.

2 cov txiaj ntsig thiab kev txheeb xyuas

Nws paub los ntawm cov txiaj ntsig tau hais tias acteoside muaj cov nyhuv proapoptotic. Kev txheeb xyuas ntawm cov txiaj ntsig ntawm cell apoptosis tau pom tias apoptotic tus nqi ntawm cov nas kho nrog siab, nruab nrab, thiab qis ntawm cov tshuaj acteoside feem ntau nce ntxiv, thiab qhov sib txawv piv nrog cov pab pawg ib txwm, pawg qauv, thiab pab pawg zoo yog qhov pom tseeb heev. . Nws tau qhia tias acteoside tuaj yeem ua rau cov cell apoptosis, thiab cov txiaj ntsig tau muaj zog dua li cov tshuaj tswj tau zoo alprostadil.

Cov nyhuv ntawm acteoside ntawm lub ultrastructure ntawm prostate hyperplasia hlwb tau pom los ntawm electron microscopy: nyob rau hauv ib txwm nas prostate, pom nuclei tsis zoo, nucleoli yog pom tseeb, thiab cov nuclear chromatin faib yog txawm; Qhov ntxhib endoplasmic reticulum tau zoo ib yam ntawm qhov loj me, teeb tsa kom zoo, tsis muaj qhov tshwm sim vacuolization, tsis muaj ntiv tes zoo li microvilli protrusions ntawm lub epithelial nto, tsis muaj qhov tshwm sim ntawm mitochondrial o tshwm sim, thiab muaj ob peb luminal secretions. Hauv pawg qauv, cov nuclei ntawm prostate hlwb tau pom tseeb deformed thiab shrunken, thiab cytoplasm muaj ntau; Cov tawv nqaij endoplasmic reticulum tau dilated heev thiab vacuolated heev, thiab cov vesicles tau ntim nrog high-density protein granules; Mitochondria tau me ntsis o nrog cov kua qaub me me hauv cov lumen thiab cov granules secretory tau pom hauv qee lub hlwb. Hauv cov nas kho nrog acteoside, cov nuclei tsis zoo piv nrog cov qauv hauv pawg, nrog me ntsis condensed nuclei, nce nuclear compactness, heterochromatin tsub zuj zuj, qee qhov vacuoles hauv cytoplasm, thiab tsawg vacuoles nyob rau hauv ntxhib endoplasmic reticulum; Hauv cov tshuaj qis qis qis qis, cov nucleus me me me me, tsis xwm yeem, thiab cytoplasm muaj ntau dua, thiab qhov ntxhib endoplasmic reticulum raug txo.

3 Kev sib tham

Kev tshawb nrhiav ntau ntawm apoptotic hlwb los ntawm FCM yog qhov yooj yim, nrawm, thiab lub hom phiaj, thiab tuaj yeem ua nrog cov kev ntsuas ntau qhov ntsuas, muab kev nkag siab tob txog kev hloov pauv hauv apoptosis thiab nws cov txheej txheem tswj tau.

Apoptosis yog ib qho programmed cell tuag txheej txheem nyob rau hauv multicellular kab mob rau cov kev cai ntawm kab mob kev loj hlob, thiab kev saib xyuas ntawm homeostasis txiav txim los ntawm noob codes. Apoptotic hlwb muaj lawv cov yam ntxwv morphological thiab lom: pyknosis ntawm chromatin nyob rau hauv lub nucleus, dav degradation ntawm DNA so, kev shrinkage ntawm cell, shrinkage ntawm cell membrane nrog txheej txheej enveloping organelles, thiab fragments ntawm nucleus tsim cov yam ntxwv apoptotic lub cev, thiaj li hais tias apoptotic hlwb tsis tsuas yog khaws cia cov cell membrane qauv thiab muaj nuj nqi tab sis kuj organelle qauv kev ncaj ncees, uas txawv ntawm cell necrosis. Cov txheej txheem molecular ntawm apoptosis tseem tsis tau paub meej txog niaj hnub no, thiab cov kev tshawb fawb tau pom tias ntau hom noob tau koom nrog kev tswj hwm cov noob koom nrog hauv apoptosis.

Apoptosis yog tam sim no muaj kev txaus siab ntau dua rau cov txheej txheem ntawm BPH, uas yog genetic encoded thiab ua rau lub cell tswj kev tuag ntawm tes los ntawm kev teb rau cov cim tshwj xeeb. Tom qab cov prostate loj hlob mus rau cov neeg laus, qhov kev loj hlob ntawm cov hlwb prostate yog khaws cia ntawm 1 feem pua ​​- 2 feem pua ​​​​los ntawm kev sib npaug ntawm tus nqi ntawm apoptosis, thiab kev cuam tshuam ntawm qhov nyiaj tshuav no tau ntev tau suav tias yog qhov ua rau BPH. . Raws li ib txwm muaj, cov tshuaj estrogen mus rau androgen theem nyob rau hauv lub prostate thiab ib txwm cycling yog lub xeev ntawm homeostasis uas muaj nyob nruab nrab ntawm proliferative thiab apoptotic txheej txheem. Thaum kev loj hlob tsis muaj qhov sib npaug nrog apoptosis, nws ua rau muaj kev loj hlob ntxiv thiab kev loj hlob ntawm cov qog, feem ntau yog los ntawm kev nthuav dav ntawm epithelial cell septa. Kev cuam tshuam ntawm qhov nyiaj tshuav no yuav tshwm sim los ntawm kev hloov pauv hauv androgen qib lossis ua haujlwm; Cov lus teb kuj tseem tuaj yeem tshwm sim los ntawm kev txhawb nqa ntawm DHT los ntawm AR lossis los ntawm kev hloov pauv hauv kev loj hlob theem kho los ntawm DHT. Nyob rau hauv txoj kev tshawb no, FCM tau ua los xyuas cov cell apoptosis, thiab nws tau pom tias tus nqi ntawm apoptosis nyob rau hauv prostate ntawm nas muab cov koob tshuaj ntau ntawm acteoside tau nce ntau dua piv nrog cov qauv pawg. Electron microscopy qhia tias cov nucleus ntawm prostate hlwb nyob rau hauv cov qauv pab pawg neeg yog deformed, cov ntxhib endoplasmic reticulum yog heev dilated nyob rau hauv vesicles, lub vesicles tau sau nrog high-density protein hais, thiab vacuolization yog pom tseeb; Mitochondria tau mob me me, ua raws li cov lus ceeb toom los ntawm cov kev tshawb fawb ntsig txog

Qib ntawm prostatic hyperplasia hauv nas tau txo qis tom qab kev cuam tshuam ntawm acteoside. Cov pab pawg neeg noj tshuaj ntau kuj tau tsim cov cim qhia ntxov ntawm apoptosis: lub nuclei ntawm prostate hlwb tseem nyob ruaj khov, thiab endoplasmic reticulum vacuoles tau txo qis. Cov nuclei tau condensed nrog nce nuclear compactness thiab heterochromatin tsub zuj zuj, zoo ib yam li cov yam ntxwv ntawm cov hlwb los ntawm post-castration prostate cov ntaub so ntswg nyob rau hauv nas. Nws tau qhia tias acteoside tuaj yeem ua rau apoptosis hauv nas nrog prostatic hyperplasia, tswj thiab kho qhov sib npaug ntawm apoptosis thiab proliferation ntawm prostate hlwb, thiab ua rau muaj kev cuam tshuam rau kev loj hlob ntawm prostate hlwb. Cov txiaj ntsig ntawm kev tshawb fawb tam sim no thiab cov kev tshawb fawb yav dhau los qhia tias inhibition ntawm prostate hyperplasia los ntawm Cistanche tshuaj tivthaiv, acteoside, tuaj yeem cuam tshuam nrog kev txhawb nqa cell apoptosis thiab attenuating AR thiab tgf-ur1 overexpression, thiab cov ncauj lus kom ntxaws tseem yuav tau tshawb xyuas tag nrho.