Lub Cim Txhim Kho Thiab Tiv Thaiv-Alzheimer's Disease Effects Of Cistanche

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Kawm txog kev tiv thaiv tag nrho Glycosides ntawm Cistanche ntawm Alzheimer tus kab mob qauv nas

Luo Dang

[Project A: Natural Science Foundation of Xinjiang Uygur Autonomous Region (No. 200121112)]

[Director pharmacist, associate professor, Ph.D. Kev taw qhia tshawb fawb: pharmacology thiab chaw muag tshuaj]

Wu Xiaochuan Qi Gaohui Ji, Lu Shunzhong 1, Wang Jianhua 2, Wang Xiaomai 3

1 Department of Pharmacy, Thawj Tsev Kho Mob ntawm Xinjiang Medical University, Urumqi 8300542

2 Morphology Center, Tsev Kawm Ntawv Qib Siab Kev Kho Mob Sciences, Xinjiang Medical University, Urumqi 830054

3 Department of Pharmacology, Tsev Kawm Ntawv Pharmacy, Xinjiang Medical University, Urumqi 830054

Kawm covtiv thaiv cov nyhuv ntawm Total Glycosides ntawmCistanche ntawm Alzheimer's disease(AD)qauv nas. Txoj Kev: Cov nas tsuag tau txhaj nrog condensed Q amyloid (Ap25.35) mus rau hauv lub dorsal ib feem ntawm lub hippocampus siv lub hlwb stereotactic technology los replicate tus nas AD qauv. Qhov kev sim tau muab faib ua sham lag luam (yog ntim ib txwm saline), qauv (iso ntim ib txwm saline), vitamin E (40 mg / kg), thiabTag nrho Glycosides ntawm Cistanchecov koob tshuaj siab, nruab nrab, thiab qis (160.80.40 mg/kg) pawg. Kev tswj hwm intragastric tau pib 4 hnub ua ntej luam cov qauv, ib hnub ib zaug rau 14 hnub sib law liag. Kev zam tsis txhob dhia kev sim thiab kev sim hluav taws xob maze tau siv los kuaj xyuas qhov kev kawm thiab kev nco muaj peev xwm ntawm nas; cov ntaub so ntswg homogenate thiab cov ntaub so ntswg hlwb ntawm cov nas hippocampus tau npaj los kuaj xyuas cov haujlwm ntawm acetylcholinesterase (AchE) hauv hippocampus cov ntaub so ntswg ntawm nas, thiab cov nas tau pom nyob rau hauv electron microscope Lub intracellular C cov ntsiab lus nyob rau hauv hippocampal CA1 cheeb tsam. Cov txiaj ntsig: Piv nrog rau pawg neeg ua haujlwm sham, cov nas hauv pawg qauv tau siv sijhawm ntev dua cov tshuaj tiv thaiv thiab kev nce ntxiv ntawm cov kev ua yuam kev (corpse VO.O1), hippocampal AchE kev ua tau zoo tau txhim kho (corpse V0.01), thiab intracellular C cia cov ntsiab lus nyob rau hauv hippocampal CA1 cheeb tsam nce. Piv nrog rau cov qauv pab pawg, cov tshuaj tiv thaiv lub sij hawm ntawm nas nyob rau hauv lub siab, nruab nrab, thiab qis pawg ntawm Tag Nrho Glycosides ntawm Cistanche tau luv luv, tus naj npawb ntawm qhov yuam kev tau txo qis (P<0.01), and="" the="" ache="" activity="" in="" the="" hippocampus="" was="" significantly="" weakened="" (fv0.01="" or="" household="" v0.05),="" the="" intracellular="" cf+="" content="" in="" the="" ca1="" area="" of="" the="" hippocampus="" was="" significantly="" reduced.="" conclusion:="">Tag Nrho Glycosides ntawm Cistanche tuaj yeem txo qhov kev ua haujlwm AchE thiab Ca2 ntxiv rau cov ntsiab lus hauv hippocampus ntawm AD qauv nas, tswj cov qib ntawm acetylcholine (Ach) hauv lub hlwb thiabtxhim kho lub peev xwm ntawm kev kawm thiab kev nco.

Ntsiab lus:Tag nrho Glycosides ntawm Cistanche; Alzheimer tus kab mob; Acetylcholinesterase; Kev kawm thiab kev nco muaj peev xwm; Calcium

Alzheimer's Disease (AD)yog ib tug kab mob ntawm lub hlwb degenerative uas tshwm sim thaum muaj hnub nyoog laus thiab yog tus cwj pwm los ntawm kev loj hlob ntawm kev txawj ntse thiab kev nco tsis zoo. Nws cov kev hloov pauv pathological feem ntau tshwm sim los ntawmsenile plaques(SP),neurofibrillary tangles(NFT), thiabneuron poob. Lub pathogenesis ntawm huab cua AD yog qhov nyuaj heev, thiab nws feem ntau cuam tshuam nrog amyloid boil (A0gQ, tau protein, presenilin, oxidative stress). Tej yam xws li stimulation thiab apoptosis muaj feem xyuam rau O. Ntawm lawv, A025-35 txoj kev xav (Amyloidcascade hypothesis) occupies ib qho tseem ceeb txoj hauj lwm nyob rau hauv lub pathogenesis ntawm AD. Qhov kev hloov pauv loj tshaj plaws uas ua rau AD tshwm sim hauv cholinergic system hu ua A. Chen feem pua ​​tuaj yeem ua rau Acetylcholinesterase (AchE) kev ua kom txo tau cov ntsiab lus ntawm acetylcholine (Ach) hauv lub hlwb thiab txo qhov peev xwm ntawm kev kawm thiab kev nco. Txoj kev loj hlob thiab kev ua haujlwm ntawm lub paj hlwb cholinergic yog tswj hwm los ntawm cov concentration ntawm intracellular Ca" hu ua Cistanches Herba. Feem ntau hu ua Dayun, ib tsob nroj hauv tsev neeg Lyndangaceae yog ib hom tshuaj suav tshuaj tonic uas muaj lub luag haujlwm ntawm nourishing raum yang. , nourishing essence thiab ntshav, moisturizing cov hnyuv thiab laxative, anti-aging, thiab lwm yam. pob (Glycosides ntawm Cistanche Herba, Tag Nrho Glycosides ntawm Cistanche) los ntawm sab qaum teb ntawm Xinjiang Extracted los ntawm Xinjiangcistanche tubulosa, cov khoom tseem ceeb yogCistanche Tag Nrho Glycosides, uas muajcov teebmeem ntawm kev tiv thaiv myocardial ischemia, tiv thaiv hluav taws xob,tiv thaiv oxidationtshem tawm cov dawb radicals hauv vitro,txhim kho tib neeg lub cev tiv thaiv kab mob, thiabncua kev laus."Ginseng" tau teev nyob rau hauv 2010 ib tsab ntawm Suav Pharmacopoeia. Cov hauj lwm yav dhau los ntawm pab pawg tshawb fawb no tau lees paub tiasTag nrho Glycosides ntawm Cistanche muaj kev tiv thaiv tseem ceeb ntawm neuro cytotoxicitytshwm sim los ntawm lub pob qhov txhab. Tib lub sijhawm, nws tau pom tiasTag nrho Glycosides ntawmCistanche tuaj yeem txhim kho kev kawm thiab kev nco muaj peev xwm ntawm AD nas tshwm sim los ntawm Pi 2 pob, thiab txhim kho qee yam kev hloov pauv hauv lub hlwb. Yog li ntawd, raws li qhov kev tshawb fawb no, tus sau tau kawm ntxiv txog cov txiaj ntsig ntawm Total Glycosides ntawm Cistanche ntawm AD tshwm sim los ntawm A feem pua ​​​​Yasuo. Covkev kawm thiab kev nco muaj peev xwmntawm nas thiab cuam tshuam ntawm AchE kev ua hauv lub hlwb thiab cov ntsiab lus ntawm Ca, ntxiv rau muab lub hauv paus rau cov kev kho mob ntawm ADthiab txhawb txoj kev loj hlob thiab kev siv ntawmCistanche.

1 khoom

1.1 Kev ntsuas

CKX41 hom electron microscope (Olympus, Nyiv); LSY hom hluav taws xob cua sov qhov kub thiab txias dej da dej (Beijing Medical Equipment Factory); Electronic tshuav nyiaj li cas (Beijing Sartorius Balance Co., Ltd.); LYJ-HI hom centrifugal nag lossis daus tshuab (Jiangsu Xiangshui Medical Equipment Factory); 721 hom spectrophotometer (Shanghai No. 3 Analytical Instrument Factory); WH-851 hom vortex tov khoom (Shanghai Huanqiu Physical Chemical Instrument Factory); TN-hom tais-hom torsion tshuav nyiaj li cas (Shanghai No. 2 Tianping Instrument Factory); Jiangwan kuv Hom C Lub hlwb stereotaxic ntaus ntawv (Second Military Medical University); platform dhia ntaus ntawv (Functional Experimental Center ntawm Xinjiang Medical University); peb-arm Y-hom maze (Zhangjiagang Sanxing Qhia Machinery Factory).

1.2 Cov ntaub ntawv kho mob

Cistanche extract(los ntawm Beijiang, Xinjiang) tau txheeb xyuas qhov tseeb los ntawm Thawj Kws Tshuaj Yang Jianhua ntawm Lub Tsev Kho Mob Thib Peb ntawm Xinjiang Medical University.

1.3 Cov tshuaj thiab cov tshuaj reagents

Tag nrho Glycosides ntawm Cistanche(ua los ntawm Department of Natural Medicines / Biopharmaceuticals, Tsev Kawm Ntawv Pharmacy, Xinjiang Medical University, cov khoom tseem ceeb yogPhenylethanoild Glycosides, the purity determined by UV method is >50 feem pua); A Yuqiu (American Sigma Company, batch tooj: 115k4778); Vitamin E (Vit E), Shanghai Sanwei Pharmaceutical Co., Ltd., batch tooj: 20070605); Cov khoom siv xeem AchE (Nanjing Jiancheng lub koom haum ntawm Bioengineering).

1.4 Tsiaj

60 tus neeg laus noj qab haus huv SD nas, 8, hnyav (220 ± 270) g, tau muab los ntawm Lub Chaw Tshawb Fawb Tsiaj ntawm Xinjiang Medical University (tsiaj ntawv pov thawj naj npawb: Yidongzi No. 16-003).

2 Txoj kev

2.1 Pawg thiab kev tswj hwm

Qhov kev sim tau muab faib ua 6 pawg, xws li kev ua haujlwm sham (iso ntim ib txwm saline), qauv (iso ntim ib txwm saline), Vit E (50 mg / kg), thiabTag nrho Glycosides ntawm Cistanchecov koob tshuaj siab, nruab nrab, thiab qis (160, 80, 40 mg) / kg), ig tswj tau pib 4 hnub ua ntej luam cov qauv, ib hnub ib zaug rau 14 hnub sib law liag.

2.2 Daim qauv ntawm tus qauv

Dissolve 1 mg ntawm A qhov txhab pob hauv 200 J1 sterilized physiological saline, kaw nws thiab muab tso rau hauv 37 T cell incubator, thiab tsim ib lub xeev condensed tom qab 7 hnub. Cov nas tsuag yog tshuaj loog nrog chloral hydrate (400 mg / kg) thiab niaj hnub npaj thiab tshuaj tua kab mob. Cov tawv nqaij yog txiav nyob rau hauv nruab nrab ntawm lub sab saum toj ntawm lub pob txha taub hau, tsau nrog ib tug stereotaxic ntsuas, thiab lub taub hau yog flattened. Kev txiav txim siab raws li daim ntawv qhia stereotaxic ntawm nas Lub nraub qaum ntawm txoj cai hippocampus yog 3.8 hli, 2.5 hli ntawm ib sab mus rau nruab nrab, thiab 3.0 hli hauv qab ntawm lub pob txha taub hau, uas yog qhov dorsal ntawm txoj cai hippocampus. Tshem tawm cov kua cerebrospinal, maj mam txhaj hauv 5 feeb nrog 10 microinjector, thiab tuav lub koob rau 10 feeb. Tom qab penicillin sodium 50, 000 u, ib hnub ib zaug rau 3 hnub.

2.3 Kev txheeb xyuas qhov ntsuas

2.3.1 nas 'passive zam platform dhia sim 14 hnub tom qab kev ua haujlwm, tab sis cov nas nkag mus rau hauv lub platform tshuaj tiv thaiv lub thawv, hloov mus rau ib puag ncig ntawm daim phiaj tooj liab rau 3 feeb, tig rau lub zog (90V alternating tam sim no), cov nas tau txais ib qho hluav taws xob poob siab, cov tshuaj tiv thaiv ib txwm yog dhia ntawm lub platform kom tsis txhob muaj kev ntxhov siab.

Tag Nrho Glycosides ntawm Cistanche txhim kho kev kawm thiab kev nco muaj peev xwm ntawm AD-induced nas, shortens lub sij hawm teb rau hluav taws xob stimulation, txo tus naj npawb ntawm kev ua yuam kev, thiab txo cov kev ua ntawm AchE thiab tsub zuj zuj ntawm Ca" nyob rau hauv lub hippocampal CA1 cheeb tsam. Hauv cov ntsiab lus, Tag Nrho Glycosides ntawm Cistanche muaj kev tiv thaiv kev kawm thiab kev nco tsis zoo. ntawm AD qauv nas tshwm sim los ntawm A", thiab yog li ua lub luag haujlwm hauv lubkev kho mob ntawm kev nco thiab kev paub tsis meej. Ib qho ntawm nws cov txheej txheem yuav yog tswj hwm qib ib txwm ntawm Ach hauv lub hlwb thiab txo cov ntsiab lus ntawm Ca. Nws muaj feem xyuam rau covkev kawm thiab kev nco muaj peev xwmntawm AD model nas. Peb pab pawg npaj siab yuav kawm ntxiv los ntawm molecular biology sebTag nrho Glycosides ntawm Cistanchetuaj yeem inhibit qhov hyperphosphorylation ntawm tau-protein deposition los ntawm down-regulating cov kev ua ntawm glycogen synthase kinase 30, yog li inhibit qhov deposition ntawm A thiab e, exerting nws neuron tiv thaiv nyhuv.

Cov ntaub ntawv

[1] Shi Anguo, Fei Yanqiu, An Furong. Kev nce qib hauv kev kho tshuaj ntawm Alzheimer's disease]. Advances in Pharmacy, 2000, 24(6:338).

[2] Hardy JA, Higgins GA. Alzheimer * tus kab mob: lub amyloid cascade hypothesis [JJ. Science, 1992, 256(5 054): 184.

[3] Guan Zhizhong, Qi Xiaolan. Kev tshawb nrhiav kev nce qib hauv molecular pathogenesis thiab kev kho mob Alzheimer's disease [J]. Suav Journal of Pathology, 2008, 37(1): 11.

[4] Feng Shulan, Lai Xinsheng, Gu Jihong. Kev cuam tshuam ntawm electroacupuncture ntawm kev nco tus cwj pwm thiab cerebral cortex acetylcholine thiab acetylcholinesterase hauv nas [J]. New Chinese Medicine, 2003, 8(8): 76.

[5] Siow NL, Choi RC, Xie HQ, et al. ATP induces synaptic gene expressions in cortical neurons: transduction and transcription control of P2Y1 receptors [J], Mol Pharmacological.