Qhov sib txawv ntawm cov khoom siv tshuaj lom neeg thiab cov dej num lom neeg ntawm Cov Khoom Raw Slices Thiab Wine Steam-Processed Product From Cistanche Deserticola

1. Taw qhia

Cistanche deserticolayog ib hom tshuaj suav tshuaj thiab feem ntau tau siv los ua tonic hauv Suav teb thiab Nyij Pooj tau ntau xyoo, feem ntau hu ua "Desert ginseng."Cistanche deserticolathawj zaug tau sau tseg raws li qib siab tshaj plaws hauv "Shennong's Herbal Classic of Materia Medica (Shen Nong Ben Cao Jing)" nyob rau xyoo 100 BC thiab siv los kho ntau yam kab mob xws li raum tsis muaj zog, tsis muaj zog, poj niam tsis muaj menyuam, mob leukorrhea, profuse metrorrhagia (dawb) , mob khaub thuas hauv lub duav thiab hauv caug, thiab cem quav ntev hauv cov neeg laus [1]. Txog tam sim no, ntau lub ntsiab lus tau raug cais tawm, xws li polysaccharides [2, 3], phenylethanoid glycosides (PhGs) [1, 4], iridoids [1] thiab ligands [1, 5]. Ntawm lawv,PhGs thiab polysaccharidesyog lub ntsiab biologically active Cheebtsam nyob rau hauvCistanche deserticola[6–9]. Cov kev sim tshuaj niaj hnub no tau ua pov thawj tiasCistanchedeserticolatuaj yeem txhim kho kev tsim cov testosterone thiab tiv thaiv cov phev, txhawb kev loj hlob ntawm tes, thiab txhim kho cell ciaj sia, nthuav qhia cov kev ua ub no rau kev sib deev potency, txhim kho kev nco, tiv thaiv kev laus, dawb radical scavenging, thiab neuroprotection [10-13].

NATURAL CISTANCHE TUBULOSA TRADITIONAL CHINESE HERBS PHGS75% ECH 30% ACT 12%

Txhawm rau txo cov tshuaj lom thiab / lossis txhim kho cov teebmeem, feem ntau ntawm cov tshuaj suav tshuaj ntsuab yuav tsum tau ua tiav ua ntej yuav tshuaj. Cov txheej txheem ib txwm muaj xws li kev ntxuav, dej txheej txheem (sib tsoo hauv dej, steaming, thiab roasting), thiab cov txheej txheem dawb (kua sov nrog cawv, vinegar, ntsev, los yog zib ntab) [14]. Thaum lub sijhawm ua haujlwm, cov khoom siv tshuaj yuav raug hloov pauv: cov ntsiab lus txheeb ze ntawm qee yam khoom yuav raug hloov lossis cov khoom tshiab yuav raug tsim [15]. Cov txheej txheem ntawm Cistanche deserticola muaj keeb kwm ntev thiab Cistanche deserticola yuav tsum tau ua tiav los ntawm kev ntub dej hauv cov cawv txiv hmab, thiab steaming raws li tau piav qhia hauv Suav Pharmacopeia (2015 tsab) [16]. SPW tau raug pov thawj los cuam tshuam lawv cov teebmeem ntawm lub raum meridians raws li kev xav ntawm cov tshuaj suav tshuaj (TCM) [17]. WSCD yog thawj zaug sau tseg hauv "Lei Gongs Treatise ntawm Kev Npaj thiab Boiling ntawm Materia Medica (Lei Gong Pao Zhi Lun)" nyob rau sab qaum teb thiab yav qab teb Dynasties ntawm Tuam Tshoj. WSCD zoo dua siv los kho lub raum tsis zoo thiab tiv thaiv cov phev raws li sau tseg hauv "Taiping Shenghui Fang" hauv Song Dynasty ntawm Tuam Tshoj. Txawm li cas los xij, tsis muaj kev tshawb fawb sib piv ntawm cov tshuaj lom neeg ntawm RCD thiab WSCD txog tam sim no.

Kev tshawb nrhiav tshuaj niaj hnub no tau qhia tias WSCD tuaj yeem ua kom lub raum kom ruaj ntseg los ntawm kev txhawb nqa lub hypothalamus-pituitary-gonad axis nrog ntau qib thiab siv rau kev kho mob ntawm lub raum tsis muaj zog xws li tsis muaj lub raum-yang [18]. Txawm li cas los xij, txog tam sim no, qhov sib txawv ntawm kev ua haujlwm lom neeg ntawm RCD thiab WSCD tsis tau tshawb fawb. Tsis ntev los no, txhawm rau pab txhawb txoj kev tshawb fawb, lub raum-yang deficiency tsiaj qauv tau muab luam tawm los ntawm kev txhaj tshuaj nas nrog cov tshuaj hydrocortisone siab, uas cov nas yuav pom cov tsos mob zoo ib yam li cov uas tau piav qhia hauv lub raum-yang deficiency ntawm TCM [19, 20] . Cov nas uas muaj lub raum-yang tsis muaj peev xwm ib txwm muaj qee cov tsos mob xws li poob phaus, noj zaub mov kom tsawg, tsis muaj zog, haus dej ntau ntxiv, thiab kev ua haujlwm tsawg. Raws li tus qauv tsiaj no, qhov sib txawv ntawm kev noj qab haus huv-mob raum cuam tshuam ntawm RCD thiab WSCD tau tshawb pom thiab tseem qhia txog qhov tseem ceeb ntawm cov txheej txheem Suav tsoos.

2. Cov ntaub ntawv thiab cov txheej txheem

2.1. Khoom siv thiab tshuaj

Cov txheej txheem tshuaj xws liacteoside, isoacteoside, echinacoside, cistanoside A, thiab 2- acetylacteosidetau yuav los ntawm Shanghai Yuanye Biotechnology Co. Ltd (Shanghai, Suav). Cistanoside F, cistanoside C, osmanthuside B, thiab tubuloside B tau yuav los ntawm Chengdu Pfeide Biotechnology Co. Ltd (Chengdu, Tuam Tshoj) Lub purity ntawm tag nrho cov qauv yog tsis tsawg tshaj li 98%. HPLC-qib methanol thiab acetonitrile tau yuav los ntawm Aladdin Chemistry Inc. (Shanghai, Suav). Deionized dej tau txais los ntawm MilliQ50 SP Reagent Water System (Bedford, MA, USA) los npaj cov qauv thiab cov kev daws teeb meem ntawm lub xov tooj. Tag nrho lwm cov organic solvents siv nyob rau hauv txoj kev tshawb no yog ntawm analytical qib thiab yuav los ntawm Shanghai Chemical Co. Ltd (Shanghai, Suav teb).

2.2. Cov Qauv Sau

Tag nrho cov Cistanche deserticola nyoos (7 batch ntawm cov qauv) tau sau los ntawm Inner Mongolia Medical University los ntawm Inner Mongolia thiab Ningxia xeev. Txhua tus raug txheeb xyuas tias yog cistanche deserticola YC Ma los ntawm Xiaoqin Wang, tus xibfwb ntawm Pharmacognosy Department hauv Inner Mongolia Medical University. Daim ntawv pov thawj tau muab tso rau hauv Tsev Kawm Ntawv Pharmacy, Inner Mongolia Medical University. Tom qab sau, cov inflorescences ntawm Cistanche deserticola raug tshem tawm, stems raug txiav thiab qhuav nyob rau hauv chav tsev kub nrog huab cua, thiab ces cov slices tau random muab faib ua ob pawg nyob rau hauv txhua batch: ib tug yog RCD thiab lwm yam yog siv rau kev npaj ntawm WSCD. .

Lub WSCD tau npaj nyob rau hauv lub chaw kuaj mob raws li Suav pharmacopeia (2015 tsab) [16], uas txhais tau hais tias cov hlais RCD tau soaked nyob rau hauv mov wine nrog ib tug kaw ntim rau 6h kom txog thaum mos, steamed nyob rau hauv ib da dej rau ib tug ntxiv 12h mus txog rau thaum lub sij hawm. nto yuav dub, thiab qhuav nyob rau hauv chav tsev kub nrog cua.

2.3. Kev Ntsuam Xyuas Cov Ntsiab Lus Tseem Ceeb (PCA)

Tus Thawj Saib Xyuas Kev Tshawb Fawb (PCA) yog cov txheej txheem sophisticated dav siv los txo qhov ntev ntawm cov teeb meem sib txawv. Nws txo qhov loj me ntawm cov ntaub ntawv qub uas tau teeb tsa los ntawm kev piav qhia txog kev sib raug zoo ntawm ntau qhov sib txawv ntawm cov ntsiab lus me me ntawm cov hauv paus ntsiab lus yam tsis muaj cov ntaub ntawv tseem ceeb. Hauv txoj kev tshawb no, qhov sib txawv ntawm RCD thiab WSCD tau ua los ntawm PCA tsis muaj kev saib xyuas siv SIMCA 13.0 software raws li cov txheeb ze ncov thaj chaw hauv HPLC chromatography. Nrog kev pab los ntawm PCA, cov tshuaj tseem ceeb cuam tshuam rau kev faib tawm ntawm cov qauv sib txawv tau pom.

2.4. Kev npaj ua qauv

2.4.1. Extract Kev Npaj rau Tsiaj

RCD thiab WSCD raug xaiv los ntawm cov qauv sau thiab npaj raws li tau piav qhia hauv Tshooj 2.2.

Cua qhuav thiab hlais RCD yog hmoov los ntawm pulverizer (FW135, Tianjin Taisite Instrument Co., Ltd.), raug hnyav 1.0 kg, thiab soaked nyob rau hauv 50% ethanol rau 30 min, thiab cov piv ntawm cov nroj tsuag / ethanol siv yog 1/10 (w / w). Thiab tom qab ntawd, nws tau muab rho tawm hauv qab reflux ob zaug rau 1 teev txhua lub sijhawm. ob lub extracts tau muab tso ua ke thiab lim thiab cov ethanol tau rov qab los ntawm qhov txo qis ntawm 60∘ C. te Tag nrho cov extract ntawm PhGs tau qhuav hauv lub tshuab nqus tsev ntawm 60∘ C thiab purified los ntawm macroporous resin. Thaum kawg, PhG extract ntawm RCD tau txais thiab raug ntsuas.

PhG extract ntawm WSCD tau txais raws li cov txheej txheem saum toj no.

cov sediments los ntawm kev rho tawm PhGs yog cua-qhuav thiab decoction ob zaug rau 1.5 h txhua zaus nrog 20 zaug dej. Ob qhov kev rho tawm tau muab tso ua ke thiab centrifuged ntawm 4000 rpm rau 10 min, thiab cov supernatant yog concentrated thiab precipitated nrog 95% ethanol. Tom qab centrifugation, cov precipitate tau qhuav hauv lub tshuab nqus tsev ntawm 60∘ C. Thaum kawg,polysaccharide extractntawm RCD tau txais thiab ntsuas qhov tseeb.

Lub polysaccharide extract ntawm WSCD tau txais raws li cov txheej txheem saum toj no.

Cov PhGs thiab polysaccharide rho tawm ntawm RCD thiab WSCD tau sib xyaw suspensions hauv dej, ntsig txog, thaum cov nas tau kho qhov ncauj.

2.4.2. Qauv Npaj rau Kev Txiav Txim PhGs

Cov PhGs rho tawm hauv Tshooj 2.4.1 tau raug hnyav ntawm 0.15 g thiab muab rho tawm los ntawm ultrasonication nrog 50mL 50% aqueous methanol tov rau 40 min. Tom qab txias, qhov poob ntawm qhov hnyav tau ntxiv nrog 50% methanol. Tag nrho cov qauv thiab cov kuab tshuaj tau lim los ntawm 0.45 ?m membrane ua ntej kev tshuaj xyuas. Cov ntsiab lus ntawm plaub PhGs hauv RCD thiab WSCD tau txiav txim siab siv HPLC, thiab tag nrho PhGs tau txiav txim siab siv UV spectrophotometric.

2.4.3. Qauv Kev Npaj rau Kev Txiav Txim ntawm Polysaccharide

Lub polysaccharide extracts nyob rau hauv Tshooj 2.4.1 tau raug hnyav li 0.10 g thiab muab rho tawm los ntawm ultrasonication nrog 50 mL dej kub rau 40 min; Tom qab txias, qhov poob ntawm qhov hnyav tau ntxiv nrog dej. Tag nrho cov qauv thiab cov kuab tshuaj tau lim los ntawm 0.45 ?m membrane ua ntej kev tshuaj xyuas.

2.5. Chromatographic mob ntawm HPLC thiab txiav txim plaub PhGs

Kev sib cais chromatographic tau ua nyob rau hauv UltiMate 3000 HPLC system (Thermo Fisher Scientific, USA), nruab nrog lub twj tso kua mis dual-gradient, ib sab hauv, thiab DAD detector . Cov ntaub ntawv tau sau thiab ua tiav siv ChromeLeon Chromatography Data System. Cov qauv tau muab cais rau ntawm Agilent Zorbax SB-C18 (250mm × 4.6 mm, 5 ?m) nrog C18 guard kem (4.6mm × 12.5 mm, 5 ?m). Cov theem txawb muaj xws li acetonitrile (A) thiab 0.1% phosphoric acid tov (B) ntawm tus nqi ntws ntawm 1 mL / min. gradient elution yog raws li nram no: pib 0–13 min, linear hloov ntawm AB (5:95, v/v) rau AB (15:85, v/v); 13–25 min, linear change to AB (20:80, v/v); 25–43 min, linear change to AB (25:75, v/v). Lub wavelength ntawm lub ntes tau saib xyuas ntawm 330 nm. Cov kab ntsuas kub tau teem rau ntawm 30 ∘ C thiab cov qauv ntim tau txhaj yog 10µL [21]. Cov kev daws teeb meem uas muaj plaub tus qauv siv tau npaj los ntawm kev ua kom tiav cov qauv siv hauv 50% methanol mus rau qhov kawg ntawm 0.20 mg / mL rau 2-acetylacteoside, 0.20 mg / mL rau acteoside, 0.05 mg / mL rau osmanthuside B, thiab 0.10 mg / mL rau isoacteoside. Cov kev daws teeb meem yog tom qab ntawd diluted mus rau tsib qhov sib txawv ntawm qhov sib txawv nrog peb qhov sib txawv los tsim cov calibration curves. Cov ntsiab lus piv txwv tau qhia hauv g / kg ntawm qhov hnyav hnyav.

2.6. Kev txiav txim siab ntawm Tag Nrho PhGs

Tag nrho cov PhGs tau txiav txim siab siv txoj kev UV spectrophotometry ntawm lub wavelength ntawm 330nm hauv UV1000 spectrophotometer (Shanghai Tianmei Scientific Instrument Co., Ltd.) Cov tshuaj npaj tau npaj los ntawm kev sib cais echinacoside cov qauv hauv 50% methanol mus rau qhov kawg concentration ntawm 0.10 mg / mL thiab tom qab ntawd diluted mus rau tsib qhov sib txawv nrog peb replicates los tsim calibration curves.

NATURAL CISTANCHE TUBULOSA rau kev txhim kho kev sib deev PHGS75% ECH 30% ACT 12%

2.7. Kev txiav txim siab ntawm Polysaccharides

Kev txiav txim siab ntawmpolysaccharidestau nqa tawm siv txoj kev phenol-sulfuric acid. 1mL ntawm cov qauv tshuaj tau muab tso rau hauv 20 mL xeem raj nrog lub iav stopper, thiab 1 mL ntawm 6% phenol tov thiab 5 mL ntawm concentrated sulfuric acid tau ntxiv thiab co rau 5 feeb. Cov sib tov tau pauv mus rau hauv ib da dej kub npau npau rau 10 min thiab txias rau chav sov kom paub txog ultraviolet. Kev nqus ultraviolet raug saib xyuas ntawm 480 nm hauv UV1000 spectrophotometer. Cov qauv siv ntawm cov dej tsis muaj dej D-glucose tau raug ntsuas kom raug thiab yaj hauv dej distilled mus rau qhov kawg ntawm 0.10 mg / mL. Tom qab ntawd cov kev daws teeb meem tau diluted mus rau tsib qhov sib txawv ntawm qhov sib txawv nrog peb qhov sib txawv los tsim cov calibration curves.

2.8. Kev sim tsiaj

2.8.1 ib. Tsiaj txhu thiab vaj tse

Txiv neej SD nas ntawm kev loj hlob ntawm kev sib deev (180-200 g) tau yuav los ntawm Xinglong (Beijing) Kev Ua Liaj Ua Teb Tsiaj (hnub nyoog: 6 lub lis piam) thiab daim ntawv tso cai kuaj tsiaj yog SCXK (Jing): 2016-0003. Tag nrho cov txheej txheem tsiaj tau pom zoo los ntawm Inner Mongolia Medical University Tsiaj Tshawb Fawb Pawg Neeg Saib Xyuas thiab ua raws li National Institute of Health cov lus qhia txog cov hauv paus ntsiab lus ntawm kev saib xyuas tsiaj (2004). Tag nrho cov tsiaj tau khaws cia rau ntawm qhov thaiv kab ke nrog qhov ntsuas kub ntawm 21-23∘ C thiab txheeb ze av noo ntawm 40-65% thiab nyob rau 12 teev tsaus ntuj / lub teeb voj voog. Cov nas tau muab ad libitumfood thiab dej thiab acclimatized rau qhov chaw saum toj no rau ib lub lim tiam.

2.8.2 ib. Dosage thiab Sampling

Cov nas tau pauv mus rau ib tus neeg lub cev metabolic tawb thiab muab faib ua 6 pawg (?=10 hauv txhua pab pawg). Lawv tau txhaj tshuaj intramuscular ntawm 15 mg / kg hydrocortisone sodium succinate (yuav los ntawm Tianjin Biochemical Pharmaceutical Co., Ltd., Tianjin, Suav teb) rau 2 lub lis piam tshwj tsis yog rau pawg 1 uas tau txhaj nrog qhov sib npaug ntawm lub cev saline. Hnub tim 14, lub cev qhov hnyav, kev noj zaub mov, noj dej, ntim zis, thiab kev ua ub ua no nyob rau hauv 5 feeb tau sau kom paub tseeb tias lub raum yang deficiency qauv tau ua tiav. Txij hnub tim 15, 6 pawg tau kho raws li hauv qab no: pawg 1 thiab pawg 2 (cov qauv pawg, M) tau kho nrog cov ntim sib npaug ntawm cov dej distilled, pawg 3 (PhGs extract ntawm RCD pawg, PR) tau kho nrog {{12 }}.42 g / kg PhGs extract ntawm RCD, uas yog sib npaug li ntawm 1.8 g / kg ntawm raw Cistanche deserticola thiab lwm pab pawg yog tib yam, pawg 4 (PhGs extract ntawm WSCD pawg, PW) tau kho ib koob ntawm { {17}}.49 g/kg PhGs extract ntawm WSCD, pawg 5 (polysaccharide extract ntawm RCD pawg, SR) tau kho nrog {{20}}}.18 g/kg cov polysaccharide extract ntawm RCD, thiab pawg 6 yog (polysaccharide extract ntawm WSCD pawg, SW) tau kho ib koob ntawm 0.22 g / kg ntawm polysaccharide extract ntawm WSCD. Txhua tus nas raug kho los ntawm plab hnyuv ib hnub. Tom qab ib hlis kev kho mob, cov nas raug tsis kam noj zaub mov rau 12 teev ua ntej sau ntshav. Hnub tom qab, txhua tus nas raug tshuaj loog thiab muab txi. Thaum lub sij hawm kuaj, cov nas raug hnyav ib zaug ib lub lim tiam los kho cov tshuaj thiab hnyav dua ua ntej txi. Cov ntshav tau sau rau hauv Eppendorf raj nrog 10% EDTA-2Na tov. Cov ntshav tau muab cais tawm ntawm centrifugation ntawm 2000 rpm rau 15 min thiab khaws cia ntawm -80 degree rau kev siv ntxiv. Tsis tas li ntawd, lub raum, noob qes, epididymis, qog prostate, thiab cov hlab ntsha hauv lub cev tau raug tshem tawm thiab hnyav sai. Tom qab qhov hnyav, cov noob qes tau khov rau hauv cov kua nitrogen rau kev txiav txim siab ntawm SOD thiab MDA.

2.9. Hormone thiab Antioxidant Effect Analysis

Cov qib testosterone (T) thiab estradiol (E2) tau txiav txim siab hauv Qingdao Kechuang Qhov Chaw Tshawb Xyuas Zoo los ntawm radioimmunity thiab colorimetric method. Cov noob qes khov tau hnyav thiab sib xyaw nrog 20 npaug ntawm cov kua qaub txias (W / W). Cov noob qes homogenate tau los ntawm cov ntaub so ntswg homogenate hauv cov dej khov da dej tau centrifuged kom tau txais cov supernatant. Cov ntsiab lus ntawm SOD thiab MDA ntawm cov noob qes tau txiav txim siab los ntawm cov khoom siv SOD thiab MDA raws li cov lus qhia.

2.10. Kev txheeb cais

Qhov tseem ceeb ntawm qhov sib txawv ntawm cov pab pawg tau muab piv los ntawm kev sim ANOVA ib-txoj kev ua raws li Schefe qhov kev sim nrog qhov tseem ceeb txwv ntawm 0.05 siv SPSS software 25.0. Tag nrho cov ntaub ntawv tau qhia raws li qhov nruab nrab ± tus qauv sib txawv (SD) (n=3).

3. Cov txiaj ntsig

3.1. Kev Ntsuam Xyuas PhGs Hloov Tom Qab SPW Siv PCA

Txhawm rau sib piv PhGs kev hloov pauv tom qab SPW, 9 chromatographic peaks raug xaiv raws li cov yam ntxwv ncov thiab raug txheeb xyuas. Lawv cov qauv tau pom nyob rau hauv daim duab 1, qhov txheeb ze ntawm qhov chaw siab tshaj uas tau suav rau qhov kev qhia ntau. HPLC chromatogram tau qhia hauv daim duab 2, uas qhia tias cov ntsiab lus txheeb ze ntawm PhGs tseem ceeb tau hloov pauv thaum SPW. Kev tshuaj xyuas PCA ntawm thaj chaw txheeb ze ntawm 9 cov khoom tau txais rau kev ntxub ntxaug ntawm cov qauv sib txawv. RCD thiab WSCD nyob deb ntawm ib leeg hauv cov phiaj xwm ntawm cov qhab nia (Daim duab 3(a)), uas qhia tias cov qauv tau muab faib ua ob pawg. Yog li nws ntseeg tau tias cov ntsiab lus ntawm cov tshuaj lom neeg sib txawv. Txhawm rau nrhiav cov cim tshuaj muaj peev xwm rau kev ntxub ntxaug, kev tshawb fawb txuas ntxiv tau ua tiav los tsim cov khoom thauj Bi (Daim duab 3(b)). Peaks 2, 3, 4, 6, thiab 7 yuav txo qis tom qab SPW thaum Peaks 5, 8, thiab 9 yuav nce, thiab tag nrho cov ntawm lawv yog cov tseem ceeb tshaj plaws kom paub qhov txawv RCD thiab WSCD. cov ntsiab lus txheeb ze ntawm ncov 1 tsis txawv ntau tom qab SPW.

NATURAL CISTANCHE TUBULOSA rau kev txhim kho kev sib deev PHGS75% ECH 30% ACT 12%

3.2. Cov ntsiab lus ntawm Tag Nrho Polysaccharides thiab PhGs hloov tom qab SPW

Raws li pom hauv daim duab 3(b), 8 yam (5, 8, thiab 9 tau nce thiab 2, 3, 4, 6, thiab 7 poob) tau hloov pauv ntau thiab muaj kev cuam tshuam loj rau cov qauv pawg. Ntawm lawv, isoacteoside thiab Osmanthuside B tau nce ntau tshaj thaum 2? -acetylacteoside thiab acteoside txo qis tshaj plaws. Yog li, cov ntsiab lus ntawm isoacteoside, Osmanthuside B, 2? -acetylacteoside thiab acteoside tau txiav txim los ntawm HPLC. Raws li pom hauv daim duab 1, ncov 4 yog acteoside, ncov 5 yog isoacteoside, ncov 7 yog 2? -acetylacteoside, thiab ncov 8 yog osmanthuside B. Tag nrho PhGs hauv RCD thiab WSCD tau txiav txim siab siv txoj kev UV thiab tag nrho cov polysaccharides tau ua los ntawm kev siv phenol-sulfuric acid txoj kev, cov txiaj ntsig tau pom nyob rau hauv Table 1, isoacteoside, Osmanthuside B. ,tag nrho polysaccharides thiab PhGshauv WSCD tau nce ntau dua piv nrog RCD. 2? -acetylacteoside thiab acteoside tau txo qis.

3.3. Tus qauv Hydrocortisone-Induced raum-Yang Deficiency Model tau tsim

Piv nrog rau cov pab pawg ib txwm muaj, tom qab 2 lub lis piam txhaj tshuaj intramuscular ntawm 15 mg / kg hydrocortisone sodium succinate, lub cev hnyav, noj zaub mov, thiab kev ua haujlwm ntawm tus kheej tau poob qis (P<0.05) and the water intake and urine volume were increased; the results were shown in Table 2. The T and E2 levels in the serum of the model group were lower than normal group as shown in Figure 4. From the above, the kidney-yang deficiency model was successfully made.

3.4. Qhov cuam tshuam ntawm Extracts ntawm Qhov Nruab Nrab Viscera Nyhav thiab Index.

Tag nrho cov nas thiab lawv cov viscera raug ntsuas thiab tom qab ntawd ntsuas qhov nruab nrab viscera ntsuas. Piv nrog rau pawg N, pawg M tau txo qis qhov hnyav ntawm lub raum, cov hlab ntsha hauv lub raum, epididymis, thiab cov noob qes, uas kuj tau qhia tias cov qauv hydrocortisone-induced raum-yang deficiency tau ua tiav. Piv nrog rau pawg M, tag nrho cov kev soj ntsuam viscera hnyav tau nce hauv PW thiab SR pawg, PR pawg tau nce qhov hnyav ntawm cov qe ntshav, epididymis, seminal vesicle, thiab prostate gland, thiab pawg SW tau nce qhov hnyav ntawm lub raum, testicle, epididymis. , thiab seminal vesicle. Piv nrog rau cov pab pawg PR, pawg PW tau zoo dua hauv lub raum, cov hlab ntsha hauv lub cev, thiab cov qog prostate. Tsis muaj qhov sib txawv tseem ceeb ntawm SR thiab SW pawg hauv qhov hnyav ntawm cov qe ntshav, epididymis, seminal vesicle, thiab prostate caj pas. Cov txiaj ntsig tau qhia hauv Table 3.

Piv nrog rau pawg N, viscera indexes ntawm lub raum, testicle, epididymis, thiab seminal vesicle tau txo qis hauv pawg M. Piv nrog rau pawg M, pawg PW thiab SR tau nce qhov Performance index ntawm lub raum, testicle, prostate gland, thiab seminal vesicle, PR pawg tau nce qhov Performance index ntawm cov noob qes, prostate gland, thiab seminal vesicle, thiab pawg SW. tau nce qhov Performance index ntawm lub raum, testicle, thiab seminal vesicle. Piv nrog rau cov pab pawg PR, pawg PW tau zoo dua hauv lub raum, cov hlab ntsha hauv lub cev, thiab cov qog prostate. Cov pab pawg SW thiab SR muaj qhov ntsuas zoo sib xws ntawm lub raum, cov qog ntshav, cov hlab ntsha hauv lub cev, thiab epididymis. Cov txiaj ntsig tau qhia hauv Table 4.

3.5. Qhov cuam tshuam ntawm Extract ntawm Qib Hormone (T thiab E2)

Raws li pom hauv daim duab 4, piv nrog rau pawg M, qib T thiab E2 hauv txhua pab pawg kho mob (PW, PR, SW, thiab SR pawg) tau nce ntau (P<0.05). However, the PW group was better than the PR group in the level T and E2. The SR and SW groups had no significant difference.

3.6. Qhov cuam tshuam ntawm Extracts ntawm Antioxidant nyhuv

SOD yog ib qho tseem ceeb antioxidant enzyme. MDA yog cov khoom ntawm lipid peroxidation uas yog qhov taw qhia ntawm kev cuam tshuam cov qib ntawm oxidant puas. Cov ntsiab lus ntawm SOD thiab MDA cuam tshuam rau qhov muaj peev xwm oxidant thiab antioxidant. Raws li pom hauv daim duab 5, Piv nrog rau pawg M, cov nyhuv antioxidant hauv txhua pab pawg kho mob (PW, PR, SW, thiab SR) tau txhim kho. Cov pab pawg SW muaj cov nyhuv antioxidant zoo tshaj plaws.

4. Kev sib tham

Ua ntej thov kev kho mob, cov ntaub ntawv nyoos yuav tsum tau ua raws li cov txheej txheem hauv Suav teb. Steaming yog ib txoj hauv kev ua haujlwm rau qee cov tshuaj suav suav tshuaj, uas muab cov xim dub uas tsim los ntawm Maillard cov tshuaj tiv thaiv [22], nce cov nqi ntawm qee cov khoom siv bioactive [23] thiab cov dej ua tshuaj. WSCD raws li ib qho ntawm cov txheej txheem cov khoom lag luam zoo dua nyob rau hauv cov nqe lus ntawm cov khoom noj rau lub raum piv nrog RCD raws li tau sau tseg hauv 2015 tsab ntawm Suav Pharmacopoeia. Hauv txoj kev tshawb no, PCA cov txiaj ntsig tau qhia tias SPW tau hloov pauv cov tshuaj lom neeg ntawm RCD. Dab tsi ntxiv, 8 cov khoom hauv PhGs tau hloov pauv ntau. Tag nrho PhGs, tag nrho polysaccharides, isoacteoside, thiab Osmanthuside B tau nce ntau tshaj, thaum 2? -acetylacteoside thiab acteoside txo qis tom qab SPW. Cov txiaj ntsig tau qhia tias SPW tuaj yeem hloov pauv cov tshuaj lom neeg ntawm RCD. Cov ntsiab lus ntawm PhGs nrog 1,3, 4- trisubstituted glucopyranosyl moieties (xws li acteoside, 2? -acetylacteoside, thiab cistanoside C) txo qis hauv WSCD, txawm li cas los xij, cov concentrations ntawm lawv cov isomers nrog 1,3,{{13 }} trisubstituted glucopyranosyl moiety (xws li isoacteoside thiab tableside B) tau nce, uas tau qhia tias kev hloov pauv ntawm cov tshuaj lom neeg tuaj yeem tshwm sim thaum SPW, thiab cov tshuaj tiv thaiv hydrolysis yuav yog ib qho laj thawj. Cov txheej txheem kev hloov pauv tau pom muaj nyob hauv daim duab 6. Qhov nce hauv polysaccharides tom qab SPW kuj tau tshaj tawm hauv ginseng [24].

Cistanche deserticola tau sau tseg los txhawb lub raum thiab thim rov qab txo qis hauv qib testosterone thiab muaj cov nyhuv antioxidant thiab tiv thaiv kev ua haujlwm [25], thiab PhGs thiab polysaccharides yog ob lub ntsiab lus tseem ceeb hauv Cistanche deserticola. Raws li PhGs, cov isoacteoside thiab Osmanthuside B muaj cov tshuaj tiv thaiv oxidation thiab tiv thaiv kab mob [26, 27]. Yog li, kev sib piv ntawm cov tshuaj pharmacological hauv qib testosterone thiab cov nyhuv antioxidant ntawm RCD thiab WSCD tau kawm. Hauv cov qauv ntawm hydrocortisone-induced raum-yang deficiency, endocrine raug cuam tshuam thiab qib ntawm cov tshuaj hormones poj niam txiv neej tau txo qis hauv cov ntshav. Hauv txoj kev tshawb no, cov txiaj ntsig ntawm kev tshuaj ntsuam xyuas poj niam txiv neej tau pom tias RCD thiab WSCD tuaj yeem nce qib ntawm T thiab E2, thiab ob qho tib si.PhGs thiab polysaccharidespom kev txhim kho ntawm kev sib deev cov tshuaj hormones. WSCD tau zoo dua li RCD, tshwj xeeb tshaj yog nyob rau hauv PhGs extract pawg. Cov txiaj ntsig ntawm viscera indexes tau pom tias PhGs thiab polysaccharides tuaj yeem txhim kho qhov hnyav viscera thiab lawv qhov hnyav indexes. Hauv PhGs extract pawg, WSCD zoo dua RCD hauv kev nce qhov hnyav thiab qhov ntsuas ntawm lub raum thiab cov hlab ntsha hauv lub cev. Tsis tas li ntawd, Cistanche deserticola muaj cov nyhuv antioxidant tseem ceeb, tshwj xeeb tshaj yog nyob rau hauv polysaccharides extract pawg nrog WSCD. Raws li tau hais los saum no, ob qho tib si RCD thiab WSCD tuaj yeem txhim kho lub raum-yang deficiency syndrome; Txawm li cas los xij, WSCD zoo dua RCD hauv kev nce qib ntawm T, E2, qhov hnyav, thiab qhov ntsuas ntawm epididymis thiab testicle. Cov txiaj ntsig ntawm kev tshawb fawb pharmacological qhia tau hais tias SPW tau hloov cov tshuaj lom neeg ntawm PhGs thiab nce cov ntsiab lus ntawm polysaccharides, uas nyob rau hauv lem nce qib ntawm kev sib deev hormone thiab txhim kho cov nyhuv antioxidant.

5. Cov lus xaus

Txoj kev tshawb no yog txhawm rau tshawb pom qhov hloov pauv ntawm PhGs, tag nrho cov polysaccharides, thiab cov tshuaj siv tshuaj hauv RCD thiab WSCD ntawm lub raum-yang deficiency nas, tshwj xeeb tshaj yog nyob rau theem ntawm cov tshuaj hormones thiab cov nyhuv antioxidant. Txoj kev tshawb no tau pom tias cov txheej txheem ntawm cov tshuaj suav tshuaj suav tshuaj tuaj yeem hloov kho lawv cov tshuaj lom neeg thiab cuam tshuam rau lawv cov bioactivity. Nws kuj tau txais kev txhawb nqa tias cov khoom nyoos thiab cov khoom ua tiav tau sau tseg sib txawvntlyhauv tsev kho mob.

NATURAL CISTANCHE TUBULOSA rau kev txhim kho kev sib deev PHGS75% ECH 30% ACT 12%