Cov Cheebtsam Ua Haujlwm Thiab Cov Kev Ua Haujlwm Antioxidant Ntawm Cov Khoom Tshiab-txiav Cistanchedeserticola YC Ma Los Ntawm Hloov Chaw Ua Haujlwm Microporous Membrane Ntim ②
Dec 16, 2022
2 Cov txiaj ntsig thiab kev sib tham
2.1 Cov teebmeem ntawm kev kho mob sib txawv ntawm O2, CO2 ntim feem, PPO kev ua haujlwm thiab qib browning
O2 thiab CO2 concentrations yog qhov tseem ceeb tsis nyob rau hauv kev tswj huab cua. Nws tuaj yeem pom los ntawm daim duab 1A thiab B tias O2 concentration hauv pawg CK tau pom qhov sib txawv me ntsis qis dua, thaum CO2 concentration pom qhov nce zuj zus. Qhov no yog vim qhov tsis zoo ntawm huab cua permeability ntawm CK pawg, thiab cov pa roj hauv pob tau hloov pauv sai sai hauv kev ua pa ntawm kev txiav tshiab.Cistanche, thiab O2 concentration yog qhov qis tshaj nyob rau 7th hnub ntawm kev cia. Hnub 4, O2 concentration nyob rau hauv pawg M2 tau nce qeeb thiab zoo li tiaj tus. Hnub 6, O2 concentration nyob rau hauv pawg M1 tau nce qeeb thiab zoo li tiaj tus. Tej zaum nws yuav yog vim muaj cov pa oxygen permeability ntawm M2 pawg ntau dua li ntawm pawg M1, thiab kev sib npaug nrawm dua [19]. PPO yog lub ntsiab ua rau lub zog ntawm cov txiv hmab txiv ntoo thiab zaub. Nws tuaj yeem pom los ntawm daim duab 1C tias PPO kev ua haujlwm tau pom qhov sib txawv ntawm thawj qhov nce thiab tom qab ntawd txo qis thaum lub sijhawm khaws cia, thiab qhov nce ntawm PPO kev ua haujlwm hauv lub sijhawm khaws cia thaum ntxov yuav yog vim muaj kev raug mob ntawm kev ntxhov siab.Cistanche deserticolathaum lub sij hawm txiav cov txheej txheem tshiab [20]. Thaum lub sij hawm 1 mus rau 5 hnub ntawm kev khaws cia, nws cov haujlwm tau txo qis. Hnub 7, PPO kev ua haujlwm ntawm M1 kev kho mob yog 6.76 feem pua thiab 5.01 feem pua qis dua li cov kev kho mob CK thiab M2, raws li qhia tau hais tias M1 kev kho mob tuaj yeem cuam tshuam qhov nce ntawm PPO kev ua haujlwm thiab txo qis kev sib khi rau cov tshuaj phenolic. Browning yog ib qho tseem ceeb ntawm cov khoom muaj nqis ntawm cov khoom tshiab-txiavCistanche.
Fig.1 Kev cuam tshuam ntawm kev kho mob sib txawv ntawm qhov ntim feem ntawm O2 (A), CO2 (B), PPO kev ua haujlwm (C) thiab kev kawm qib siab (D) ntawm cov tshiab txiav C.deserticola
Nws tuaj yeem pom los ntawm daim duab 1D tias qhov browning degree ntawm cov tshiab-txiavCistanche deserticolanyob rau hauv cov pab pawg kho mob sib txawv tau pom tias muaj kev nce ntxiv thaum lub sijhawm khaws cia. Thaum kawg ntawm kev khaws cia, pawg kho M1 thiab M2 yog 6.56 feem pua thiab 18.03 feem pua qis dua CK pawg, raws li, thiab qib browning ntawm pawg kho M2 yog qhov qis tshaj ntawm 0.51 OD410/g . Qhov no tej zaum yuav yog vim muaj zog ua pa thiab siab PPO kev ua ntawm kev txiav tshiabCistanchenyob rau hauv thaum ntxov lub sij hawm cia, thiab ua ke ntawm browning-txog enzymes thiab phenolic tshuaj, ua rau browning. Nrog kev sib pauv roj, M1, M2
Nug kom paub meej ntxiv:
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Cov pab pawg kho mob tau ua tiav ib qho kev sib npaug sib npaug microenvironment, inhibited qhov kev siv ua pa ntawmtshiab-txiav Cistanche, slowed down lub physiological metabolic tus nqi, thiab txo cov degree ntawm daim nyias nyias lipid peroxidation [21-23]. Nrog rau kev txo qis ntawm PPO kev ua haujlwm, kev tsim cov xim av polymers raug txo. , yog li inhibiting nws browning degree. Cov huab cua permeability ntawm CK pab pawg neeg tsis zoo, anaerobic ua pa yog ib qho yooj yim tshwm sim, thiab cov kab mob tau yooj yim tsim thaum lub sij hawm cia, yog li cov degree ntawm browning yog siab dua li ntawm M1 thiab M2 kev kho mob pawg, uas cuam tshuam rau lub sensory zoo ntawm tshiab- txiav Cistanche.
Fig.2 Cov teebmeem ntawm kev kho mob sib txawv ntawm Vc cov ntsiab lus (A), tag nrho cov ntsiab lus phenol (B) thiab cov ntsiab lus flavonoid (C) ntawm cov tshiab-txiav C.deserticola
Vc yog ib qho tseem ceeb hauv cov txiv hmab txiv ntoo thiab zaub, thiab ib qho ntawm cov cim tseem ceeb cuam tshuam rau kev khaws cia zoo ntawm txiv hmab txiv ntoo thiab zaub, thiab plays lub luag haujlwm antioxidant hauv txiv hmab txiv ntoo thiab zaub. Raws li pom nyob rau hauv daim duab 2A, thaum lub sij hawm tag nrho lub sij hawm cia, Vc cov ntsiab lus nyob rau hauv cov pab pawg neeg kho mob sib txawv pom ib tug maj mam txo. Ntawm lawv, pab pawg kho mob M1 Vc
Cov ntsiab lus ntawm Vc hauv pawg M2 thiab CK kho mob ib txwm siab dua li ntawm pawg kho mob M2 thiab CK (P<0.05). At the 7th day of storage, the Vc contents of the M1, M2 and CK treatment groups were 1.74%, 1.62% and 1.54%, respectively, and the M1 treatment groups were M2 and CK respectively. 1.07 times and 1.13 times of the treatment group. It may be that fresh-cut Cistanchecuam tshuam los ntawm kev puas tsuaj thiab kev ua haujlwm ntawm lub cev metabolic, uas ua rau kev noj thiab oxidation ntawm Vc hauv cov ntaub so ntswg, ua rau txo qis hauv Vc cov ntsiab lus [24]. Tom qab lub microporous membrane hloov chaw ntim kev kho mob, cov pa roj hauv lub thawv ntim tau sai sai mus txog qhov sib npaug ntawm lub xeev los ntawm microporous txauv, uas inhibited lub physiological metabolic tus nqi ntawm tshiab-txiav.Cistanche deserticola, yog li qeeb oxidative decomposition ntawm Vc. Nws qhia tau hais tias kev kho M1 tuaj yeem ua rau txo qis ntawm Vc cov ntsiab lus hauv kev txiav tshiabCistanche deserticola, thiab tswj nws txoj haujlwm antioxidant zoo dua. Reche [25] pom tias los ntawm kev ncua qhov txo qis ntawm O2 thiab qhov nce ntawm CO2 hauv lub ntim, kev noj cov as-ham tuaj yeem txo qis, yog li txo qis Vc thiab tag nrho cov ntsiab lus phenolic ntawm jujube txiv hmab txiv ntoo thaum lub caij txias txias, thiab ncua sijhawm. txiv hmab txiv ntoo ripening thiab senescence.
Phenolic tshuaj muaj dav nyob hauv cov nroj tsuag thiab ua lub luag haujlwm tseem ceeb hauv cov txheej txheem ntawm cov nroj tsuag antioxidant. Raws li pom nyob rau hauv daim duab 2B, tag nrho cov ntsiab lus phenolic hauv kev kho mob sib txawv tau pom qhov sib txawv ntawm thawj qhov nce thiab tom qab ntawd txo qis. Hnub 5 ntawm kev khaws cia, tag nrho cov ntsiab lus phenolic ntawm cov pab pawg kho mob sib txawv tau mus txog tus nqi siab tshaj plaws, thiab tag nrho cov ntsiab lus phenolic ntawm pawg kho mob M1 yog 1.5 feem pua hauv M2 thiab CK kho mob pawg. 1,38x thiab 1.11x ntawm . Qhov no tej zaum yuav yog vim kev puas tsuaj ntawm lub compartmentalized qauv ntawm lub hlwb thaum lub sij hawm tshiab-txiav txheej txheem, uas ua rau ib tug nce nyob rau hauv cov ntsiab lus ntawm phenolic tshuaj [26]. Nyob rau theem tom ntej ntawm kev khaws cia, cov txheej txheem kev laus ntawm cov tshiab-txiavCistanchetau nruj, thiab tag nrho cov ntsiab lus phenolic maj mam txo. Ntawm lawv, cov concentration ntawm O2 nyob rau hauv cov pob khoom M1 thiab M2 tau nce, thiab oxidation ntawm phenolic tshuaj nrawm. Piv nrog rau kev kho M1, huab cua permeability ntawm M2 tau zoo dua, thiab oxidation tus nqi ntawm cov tshuaj phenolic tau nrawm dua. Thaum kawg ntawm kev khaws cia, tag nrho cov ntsiab lus phenolic hauv pawg kho M1 tseem yog qhov siab tshaj plaws. Nws tau qhia tias kev kho M1 tuaj yeem tswj hwm tag nrho cov ntsiab lus phenolic zoo dua hauv kev txiav tshiabCistanche deserticola.
Vc, tag nrho cov phenols thiab flavonoids yog ntuj antioxidants nyob rau hauv txiv hmab txiv ntoo thiab zaub, uas muaj peev xwm tswj tau cov antioxidant muaj peev xwm ntawm lub cev. Raws li pom nyob rau hauv daim duab 2C, thaum lub sij hawm cia, cov ntsiab lus flavonoid nyob rau hauv ntau pawg kho mob thawj zaug nce thiab ces txo.
Tus nqi siab tshaj plaws tau tshwm sim ntawm , thiab cov ntsiab lus flavonoid hauv pawg kho M1 yog qhov siab tshaj thaum khaws cia. Hnub 7 ntawm kev khaws cia, cov ntsiab lus flavonoid hauv pawg kho M2 thiab CK yog 41.41 feem pua thiab 10.10 feem pua qis dua cov hauv pawg kho M1, raws li. Nws qhia tau hais tias kev kho M1 tuaj yeem ua rau txo qis ntawm cov ntsiab lus flavonoid.
2.3 Cov nyhuv ntawm kev kho mob sib txawv ntawm tag nrho cov ntsiab lus polysaccharide
Cov nroj tsuag polysaccharides muaj kev ua haujlwm ntawm inhibiting dawb radicals los yog tshem tawm cov dawb radicals, thiab yog ib qho ntawm cov khoom xyaw tseem ceeb hauv cov nroj tsuag. Raws li pom nyob rau hauv daim duab 3, thaum lub sij hawm cia, tag nrho cov ntsiab lus polysaccharide ntawm tshiab-txiavCistanche deserticolahauv cov pab pawg kho mob sib txawv tau pom qhov kev poob qis zuj zus, thiab CK pawg tau txo qis tshaj plaws. Qhov no tej zaum yuav yog vim ceev ceev ntawm cov as-ham thiab substrate organic acids tom qab cov tshiab-txiav kev kho mob ntawmCistanche deserticola, thiab degradation ntawm polysaccharides rau hauv monosaccharides [27], ua rau txo qis ntawm tag nrho cov ntsiab lus polysaccharide. M1 kev kho mob tuaj yeem cuam tshuam cov metabolism hauv lub cev ntawm cov txiav tshiabCistanche deserticolathiab maj mam degradation ntawm tag nrho polysaccharides. Hnub 7 ntawm kev khaws cia, tag nrho cov ntsiab lus polysaccharide ntawm cov tshiab-txiavCistanchehauv pawg kho M1 yog 25.66 mg DE / g DW, uas yog 6.43 feem pua siab dua li hauv M2 (24.11 mg DE / g DW) thiab CK (22.42 mg DE / gDW) pawg kho mob , 14.45 feem pua. Nws tau qhia tias kev kho M1 tuaj yeem txo qhov poob ntawm tag nrho cov ntsiab lus polysaccharide hauv kev txiav tshiabCistanche deserticola.
Fig.3 Cov teebmeem ntawm kev kho mob sib txawv ntawm cov ntsiab lus polysaccharide ntawm cov tshiab-txiav C.deserticola
2.4 Cov teebmeem ntawm kev kho mob sib txawv ntawm cov ntsiab lus ntawm echinacoside thiab verbascoside
Daim duab 4A yog tus qauv chromatogram, thiab daim duab 4B yog tus qauv chromatogram; Table 1 qhia txog qhov kev siv tau zoo sib xws rauechinacoside thiab verbascoside. Lub calibration nkhaus muaj ib tug zoo linear regression nyob rau hauv qhov ntau (R
2=0.echinacoside thiab verbascosidehauv Cistanche qauv hmoov ib txhij. Echinacoside thiab verbascoside yog cov khoom siv tseem ceeb hauvCistanche deserticola, uas yog phenylethanol glycosides thiab muaj cov teebmeem antioxidant [28]. Nws tuaj yeem pom los ntawm daim duab 5A thiab B uas cov ntsiab lus ntawmechinacoside thiab verbascosidenyob rau hauv ntau pab pawg kho mob pom ib tug maj mam downward trend, thiab downward trend tsis tseem ceeb. Thaum lub sij hawm tag nrho lub sij hawm cia, cov ntsiab lus ntawmechinacoside thiab verbascosidehauv pab pawg kho mob M1 yeej ib txwm siab dua li hauv CK pawg. Nyob rau 7th hnub ntawm cia, cov ntsiab lus ntawmechinacoside cov tshuajhauv Cistanche tshiab hauv pab pawg kho M1 yog 5.92 mg / g, uas yog 1. mg / g , uas yog 0.49 feem pua thiab 1.47 feem pua siab dua cov pab pawg kho mob M2 thiab CK, feem. Qhov no tej zaum yuav yog vim Cistanche deserticola cov nroj tsuag muaj enzymes cuam tshuam rau hydrolysis ntawm phenylethanol glycosides, thiab phenylethanol glycosides yuav hydrolyzed rau hauv me me molecular tshuaj nrog rau kev nce lub sij hawm cia, ua rau txo qis hauv lawv cov ntsiab lus [29,30] thiab cuam tshuam rau kev ua haujlwm ntawmCistanche deserticolapoj niam deev. Hauv qhov kev sim no, tshiab-txiavCistanche deserticolatau muab tso rau hauv ib puag ncig ntawm 4 degree, thiab qhov kub tsis tshua muaj qhov cuam tshuam rau kev ua haujlwm ntawm hydrolase ntsig txog phenylethanol glycosides, yog li txo qis qib ntawm hydrolysis ntawm phenylethanol glycosides [31] thiab tswj lawv cov ntsiab lus zoo dua. Nyob rau tib lub sijhawm, M1 kev kho mob tuaj yeem ua rau cov pa roj hauv lub thawv ntim kom ncav cuag qhov sib npaug, inhibit qhov ua pa ntawm Cistanche tshiab, thiab ua rau lub neej ua haujlwm qeeb, thiab cov roj tau pauv los ntawm micropores los tiv thaiv kev ua pa anaerobic, yog li ua kom qeeb. pH tus nqi ntawm Cistanche tshiab. Zoo tswj cov stability ntawmphenylethanol glycosides[32]. Cov txiaj ntsig tau pom tias kev kho M1 tuaj yeem tswj tau cov ntsiab lus ntawmechinacoside thiab verbascosidenyob rau hauv tshiab-txiavCistanche deserticola, tswj nws cov khoom ua haujlwm, thiab txhim kho nws cov nqi tshuaj.
Ib tug qauv
B txheem
Table 1 HPLC txoj kev tau siv los txiav txim qhov kev siv tau ntawm mulin thiab echinoside hauv cov txiav tshiab C.deserticola
polysaccharide
Fig.5 Cov teebmeem ntawm kev kho mob sib txawv ntawm cov ntsiab lus ntawm echinoside (A) thiab calycoside (B) hauv cov tshiab-cutC.deserticola
2.5 Cov txiaj ntsig ntawm kev kho mob sib txawv ntawm cov khoom antioxidant
DPPH, ABTS ntxiv rau radical scavenging muaj peev xwm thiab FRAP txo peev xwm yog cov cim tseem ceeb ntawm covantioxidant muaj peev xwmntawm txiv hmab txiv ntoo thiab zaub, thiab ntau dua radical scavenging tus nqi qhia muaj zogantioxidant muaj peev xwm. Raws li pom nyob rau hauv daim duab 6A thiab B, nrog rau kev nce ntawm lub sij hawm cia, tus nqi khawb ntawm DPPH thiab ABTS ntxiv rau radicals nyob rau hauv cov kev kho mob sib txawv pom ib tug sib txawv ntawm nce thiab ces txo, uas yog raws li cov kev hloov ntawm tag nrho cov ntsiab lus phenolic thiab flavonoid cov ntsiab lus. , qhia tau hais tias qhov kev tshem tawm ntawm DPPH thiab ABTS ntxiv rau cov radicals tau ze ze rau tag nrho cov phenols thiab flavonoids. DPPH radical scavenging tus nqi ntawm cov pab pawg kho mob sib txawv tau mus txog qhov kawg ntawm 5th d ntawm kev cia, ntawm cov uasDPPHradical scavenging tus nqi ntawm pab pawg kho mob M1 yog 92.38 feem pua, thaum lubDPPHradical scavenging tus nqi ntawm M2 thiab CK kho cov pab pawg yog 79.05 feem pua thiab 88.25 feem pua. Qhov no qhia tau hais tias kev kho M1 cuam tshuam qhov kev tshem tawm ntawm DPPH radicals mus rau qib sib txawv thiab muaj txiaj ntsig zoo tshaj plaws. Qhov sib txawv ntawm ABTS ntxiv rau qhov kev tshem tawm ntawm qhov chaw khaws cia zoo ib yam li ntawm DPPH radical scavenging tus nqi, nrog rau qhov siab tshaj ntawm 90.26 feem pua ntawm d 5 hauv M1 pab pawg kho mob thiab 2.28 feem pua thiab 1.70 feem pua qis dua M1 kho ntawm d 4 hauv M2. thiab pab pawg kho mob CK, raws li (P<0.05 for significant difference). It showed that M1 treatment had a significant effect on the scavenging rate of ABTS+ free radicals in fresh-cut Cistanche, uas tuaj yeem ncua kev laus oxidative ntawm tshiab-txiav Cistanche.
Cov ntsiab lus FRAP siab dua qhia tias muaj zog duaantioxidant muaj peev xwmntawm txiv hmab txiv ntoo thiab zaub. Raws li pom nyob rau hauv daim duab 6C, FRAP nyob rau hauv cov tshiab-txiav cistanches pom ib tug zuag qhia tag nrho kev poob qauv, uas yog raws li tus qauv ntawm Vc cov ntsiab lus thiab tag nrho cov ntsiab lus, qhia tias FRAP txo peev xwm yog ze ze rau Vc cov ntsiab lus thiab tag nrho cov ntsiab lus polysaccharide nyob rau hauv tshiab-txiav. cistanches. FRAP hauv pab pawg kho M1 yog 0.78 mmol/L los ntawm 7th d ntawm kev cia, uas yog 4.00 feem pua thiab 11.43 feem pua siab dua li hauv pawg kho M2 thiab CK, raws li . Cov txiaj ntsig tau pom tias kev kho M1 yog qhov zoo tshaj plaws thiab tuaj yeem txhim kho cov khoom antioxidant ntawm Cistanche tshiab. Tsis tas li ntawd, aeration ntim tuaj yeem tswj hwm kev ua haujlwm ntawm cov enzymes uas cuam tshuam nrog antioxidant, txhim kho cov antioxidant muaj peev xwm ntawm cov txiv hmab txiv ntoo, thiab txo cov qib ntawm oxidative kev nyuaj siab, yog li ncua qhov poob ntawm qhov zoo [33]. Nws tau raug pom tias cov ntsiab lus phenolic thiab flavonoid ntawm ntau cov txiv hmab txiv ntoo muaj feem cuam tshuam nrog lawv tus kheej cov khoom antioxidant [34-36]. Txoj kev tshawb fawb tam sim no tau pom tias M1 kev kho mob zoo dua khaws cov khoom xyaw tshiab ntawm Cistanche tshiab, txhim kho cov tshuaj antioxidant ntawm cov khoom tshiab-txiav.Cistanche, zoo ncua cov ntaub so ntswg laus, tiv thaiv hlwb los ntawm microbial kab mob, thiab yog li txhim kho nws resilience thiab zoo khaws cia zoo ntawm tshiab-txiav Cistanche.
3 Kev xaus
Kev kho cua sov hloov kho (6 feem pua CO2 ntxiv rau 4 feem pua O2 ntxiv rau 90 feem pua N2) ua ke nrog cov khoom ntim sib txawv tau kawm txog kev txiav tshiabCistancheM1 kev kho mob tuaj yeem cuam tshuam qhov nce ntawm PPO kev ua si thiab browning degree nyob rau hauv tshiab-txiav Cistanche, qeeb qhov nce ntawm Vc, tag nrho cov ntsiab lus Txo cov phenols, flavonoids, tag nrho cov polysaccharides, echinacoside thiab verbascoside khaws DPPH, ABTS ntxiv rau dawb radical scavenging tus nqi thiab FRAP txo peev xwm ntawm qib siab. 6 feem pua CO2 ntxiv rau 4 feem pua O2 ntxiv rau 90 feem pua N2 ntxiv rau M1 kev kho mob txhim kho cov antioxidant muaj peev xwm ntawm cov tshiab-txiav Cistanche, ua rau kom xim av thiab kev laus, thiab tswj tau qhov zoo ntawm cov tshiab-txiav Cistanche. Theoretical hauv paus.
