Thanaka (H. Crenulata, N. Crenulata, L. Acidissima L.): Kev Tshawb Fawb Txog Kev Siv Tshuaj, Tshuaj lom neeg thiab kev siv tshuaj pleev ib ce Part 2

4. Tshuaj lom neeg ntawm Thanaka

4.1. Antioxidant Kev Ua Haujlwm

Antioxidants, los yog dawb-radical scavengers, yog cov khoom uas tiv thaiv los yog qeeb ntawm lub cell puas los ntawm cov dawb radicals, uas yog ib tug tsis ruaj tsis khov molecule tsim los ntawm lub cev reacting rau ib puag ncig kev nyuaj siab, yog li tiv thaiv tib neeg noj qab haus huv [17]. Qhov chaw ntawm antioxidants tuaj yeem yog ntuj lossis dag; Txawm li cas los xij, tej yam ntuj tso cov tshuaj antioxidants los ntawm cov nroj tsuag feem ntau nyiam los ntawm tib neeg vim lawv ntseeg tias muaj kev nyab xeeb dua. Polyphenols uas muaj peev xwm pom tau nyob rau hauv feem ntau cov nroj tsuag yog suav hais tias yog ib tug zoo antioxidant, vim hais tias cov txheej txheem chemistry ntawm polyphenols muab los ntawm cov nroj tsuag yog zoo tagnrho rau tshem tawm dawb radicals, uas tau pom tias muaj ntau zoo antioxidants nyob rau hauv vitro tshaj cov vitamins E thiab C [17 ]. Antioxidants kuj tuaj yeem khaws cov zaub mov.

Glycoside ntawm cistanche tuaj yeem ua rau muaj kev ua haujlwm ntawm SOD hauv plawv thiab daim siab cov ntaub so ntswg, thiab txo cov ntsiab lus ntawm lipofuscin thiab MDA hauv txhua cov ntaub so ntswg, tshem tawm ntau yam reactive oxygen radicals (OH-, H₂O₂, thiab lwm yam) thiab tiv thaiv DNA puas. los ntawm OH-radicals. Cistanche phenylethanoid glycosides muaj peev xwm tshem tawm cov dawb radicals, muaj peev xwm txo tau ntau dua li cov vitamin C, txhim kho cov haujlwm ntawm SOD hauv cov phev ncua, txo cov ntsiab lus ntawm MDA, thiab muaj qee yam kev tiv thaiv ntawm cov phev ua haujlwm. Cistanche polysaccharides tuaj yeem txhim kho kev ua haujlwm ntawm SOD thiab GSH-Px hauv erythrocytes thiab ntsws cov ntaub so ntswg ntawm cov nas senescent sim los ntawm D-galactose, nrog rau txo cov ntsiab lus ntawm MDA thiab collagen hauv lub ntsws thiab ntshav, thiab nce cov ntsiab lus ntawm elastin, muaj Cov nyhuv scavenging zoo ntawm DPPH, ncua lub sijhawm ntawm hypoxia hauv cov nas senescent, txhim kho cov haujlwm ntawm SOD hauv cov ntshav, thiab ncua lub physiological degeneration ntawm lub ntsws hauv kev sim cov nas nrog cellular morphological degeneration, kev sim tau pom tias Cistanche muaj peev xwm antioxidant zoo. thiab muaj peev xwm los ua ib qho tshuaj tiv thaiv thiab kho cov kab mob ntawm daim tawv nqaij laus. Nyob rau tib lub sijhawm, echinacoside hauv Cistanche muaj lub peev xwm tseem ceeb los tshem tawm DPPH dawb radicals thiab tuaj yeem tshem tawm cov pa oxygen reactive, tiv thaiv dawb radical-induced collagen degradation, thiab kuj muaj kev kho zoo ntawm thymine dawb radical anion puas.

Nyem rau rou cong rong cov txiaj ntsig

【Yog xav paub ntxiv:george.deng@wecistanche.com / WhatApp:86 13632399501 】

Tag nrho cov ntsiab lus phenolic (TPC) thiab diphenyl-picrylhydrazyl (DPPH) feem ntau yog ua haujlwm los txiav txim siab cov haujlwm antioxidant ntawm cov nroj tsuag rho tawm siv lub spectrophotometer. Tus nqi ntawm phenolic cov ntsiab lus ntawm cov nroj tsuag extract yog soj ntsuam siv Folin-Ciocalteu colorimetric txoj kev. Cov qauv siv feem ntau yog gallic acid, pyrocatechol, thiab tannic acid. DPPH assay yog dav siv rau kev ua haujlwm antioxidant los ntawm kev ntsuas tag nrho cov radical scavenging peev ntawm antioxidants mus rau cov dawb radical ruaj khov, uas tom qab ntawd reacts nrog hydrogen donor compounds.

Antioxidant kev ua ub no ntawm Thanaka extracts tau soj ntsuam los ntawm ntau tus kws tshawb fawb raws li tau hais hauv Table 3. Tshwj xeeb, Wangthong li al. nyob rau hauv 2010 soj ntsuam ntau yam extracts ntawm Thanaka qia bark siv cov kuab tshuaj sib txawv xws li hexane, methanol, ethyl acetate, 85 feem pua ​​aqueous ethanol, thiab dej. Los ntawm DPPH tshuaj tiv thaiv antioxidant, lawv pom tias 85 feem pua ​​​​ethanol rho tawm ntawm Thanaka qia tawv nqaij muaj cov tshuaj tiv thaiv kab mob siab tshaj plaws thaum hexane muaj cov haujlwm qis tshaj [18]. Lawv kuj tau soj ntsuam TPC hauv Thanaka qia bark rho tawm qhov twg cov tshuaj methanol rho tawm muaj ntau tshaj plaws ntawm TPC thaum hexane extract muaj tus nqi qis tshaj TPC. Nyob rau hauv 2012, Shermin et al. kuj tau ua DPPH tshuaj tiv thaiv antioxidant ntawm Thanaka qia bark extracts ntawm cov kuab tshuaj sib txawv xws li chloroform, roj av ether, thiab methanol. Cov txiaj ntsig tau pom tias chloroform extract muaj cov tshuaj tua kab mob siab tshaj plaws ua raws li cov roj av ether, tom qab ntawd methanol [19]. Xyoo 2017, Sonawane thiab Arya tau soj ntsuam cov kev ua haujlwm antioxidant ntawm cov protein hydrolysates tau los ntawm Thanaka noob los ntawm DPPH, 2,20 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric txo antioxidant zog (FRAP) thiab hlau chelating muaj peev xwm soj ntsuam. Lawv pom tias qhov kev ntsuam xyuas DPPH tsis haum rau cov protein hydrolysates, thaum nyob rau hauv qhov kev ntsuam xyuas FRAP, kev nyeem ntawv absorbance tau nce raws li qhov concentration ntawm cov protein hydrolysates nce; Txawm li cas los xij, cov txiaj ntsig tau qis dua piv rau tus qauv Trolox. Hauv kev ntsuam xyuas ABTS thiab hlau chelating muaj peev xwm, kev sib raug zoo ntawm cov protein hydrolysates thiab antioxidant kev ua haujlwm yog linear thiab cov tshuaj tiv thaiv antioxidant tau pom tias siab dua tus qauv Trolox [20]. Tom qab xyoo 2020, Sonawane li al. kuaj Thanaka noob protein hydrolysates nyob rau hauv cia thiab xim stability ntawm anthocyanins. Lawv tau tshaj tawm tias qhov ruaj khov ntawm anthocyanin me ntsis nce ntxiv los ntawm 10 h thiab 34 min ntawm 0.12 feem pua ​​​​ntawm cov protein hydrolyzate concentration tab sis poob qis dua ntawm 2 feem pua, thaum cov xim ruaj khov ntawm anthocyanin nce ntxiv raws li qhov concentration ntawm Thanaka noob protein hydrolysates nce [21 ]. Hauv 2018, Jantarat et al. tsim plaub cov tshuaj ntsuab suav nrog Thanaka bark hmoov rau hauv lub pob tshuaj ntsuab thiab kuaj rau lawv cov kev ua haujlwm antioxidant los ntawm DPPH kev soj ntsuam uas ua rau muaj kev ua haujlwm antioxidant qis dua li tus qauv gallic acid los ntawm 40-fold [22].

4.2. Antimicrobial Activity

Nws yog tsim nyog hais tias kev lig kev cai Thanaka yog siv los kho pob txuv thiab tshuaj tua kab mob. Qhov no ua rau cov kws tshawb fawb muaj kev txaus siab los ntsuas cov tshuaj tiv thaiv kab mob ntawm Thanaka. Wangthong et al. (2010) tau ua ob qho tib si yam tsawg kawg nkaus inhibitory concentration (MIC) thiab yam tsawg kawg nkaus bactericidal concentration (MBC) assay los ntawm kev kho ntau yam hnyav extracts ntawm Thanaka qia bark mus rau Staphylococcus aureus (S. aureus) thiab Escherichia coli (E. coli) thiab siv clindamycin ua tus txheem. Cov txiaj ntsig tau pom tias tag nrho cov extracts muaj cov tshuaj tua kab mob me me uas yog 10 rau 20-fold qis tiv thaiv E. coli thiab 300-fold qis dua tawm tsam S. aureus thaum piv nrog tus qauv clindamycin [18]. Interestingly, lub pob herbal muaj plaub yam tshuaj ntsuab, piv txwv li, Andrographis paniculata, Centella Asiatica, Benchalokawichian, thiab Thanaka bark hmoov tau npaj los ntawm Jantarat li al., 2018, tau pom muaj kev tiv thaiv kab mob tiv thaiv Propionibacterium acnes (P. acnes) ntawm qhov concentration ntawm 31.25. µg/mL hauv MIC thiab MBC kev xeem [22].

4.3. Cytotoxicity thiab Cell Viability

Txij li thaum nws yog ib qho tshuaj uas siv rau ntawm daim tawv nqaij, nws yog ib qho tseem ceeb kom paub txog cytotoxicity ntawm Thanaka los txiav txim siab txog kev nyab xeeb ntawm kev siv. Wangthong et al. xyoo 2010 tau soj ntsuam ob qho tib si cytotoxicity thiab genotoxicity ntawm Thanaka qia bark extracts ntawm tib neeg melanoma A-375 kab ntawm tes. Raws li lawv qhov kev soj ntsuam, cov hmoov tawv tawv qub tsis pom muaj cov tsos mob ntawm cytotoxicity ntawm A-375 hlwb, thaum cov dej, methanol, thiab 85 feem pua ​​​​ethanol rho tawm pom cov cell cytotoxicity tsawg heev. Txawm li cas los xij, ethyl acetate, hexane, thiab dichloromethane tau pom me ntsis siab dua cytotoxicity tawm tsam A375 hlwb tab sis tag nrho, qis dua li tus qauv doxorubicin. Raws li rau genotoxicity, Wangthong li al., 2010 tau thov tias tag nrho cov extracts thiab qub bark hmoov tsis muaj genotoxicity [18]. Ma et al. (2020) soj ntsuam cytotoxicity ntawm Thanaka nplooj extract mediated tin (IV) oxide nanoparticles (SnO2 NPs) tawm tsam tib neeg mob qog noj ntshav (SiHa) cell kab thiab ua rau lub cell viability ntawm SiHa hlwb txo raws li qhov concentration ntawm Thanaka nplooj extract mediated SnO2 NPs nce. Lawv kuj tau soj ntsuam cov morphology ntawm SiHa cell los soj ntsuam cell apoptosis. SiHa hlwb tau nthuav tawm lub neej ntawm necrotic thiab apoptotic cell morphology tom qab kho SiHa hlwb nrog Thanaka nplooj SnO2 NPs rau 24 h [23].

Tsis tas li ntawd, toxicity qauv xws li Artemia salina (A. salina) brine cw thiab Culex quinquefasciatus (C. quinquefasciatus) yoov tshaj cum larvae tau ua haujlwm hauv cytotoxicity tshuaj ntsuam ntawm Thanaka extracts [14,19]. Shemin et al. (2012) soj ntsuam cytotoxicity ntawm Thanaka qia bark extracts ntawm brine cw A. salina thiab pom tias muaj me ntsis siab cytotoxicity nyob rau hauv roj av ether thiab chloroform extracts, thaum cytotoxicity tsawg heev tau pom nyob rau hauv methanol extract; Txawm li cas los xij, cytotoxicity ntawm tag nrho cov extracts yog qis dua li tus qauv vincristine sulfate [19]. Pratheeba et al. (2019) soj ntsuam kev ua haujlwm ntawm larvicidal efficacy ntawm ntau yam Thanaka nplooj extracts tiv thaiv C. quinquefasciatus. Kev tuag ntau dua tau pom nyob rau hauv C. Quinn fasciitis larvae ntawm 24 h tom qab kev kho mob nrog acetone extract ntawm Thanaka nplooj nrog lethal concentration 50 (LC50) thiab lethal concentration 90 (LC90) tus nqi qis li 1.02 mg/L thiab 1.93 mg/ L feem, ua raws li methanol extract nrog 1.13 mg / L LC50 thiab 2.24 mg / L LC90 qhov tseem ceeb, ethyl acetate extract nrog 1.81 mg / L LC50 thiab 4.14 mg / L LC90 thiab hexane extract nrog 9.74 mg / L LC50 thiab 2.3 LC90 [14].

4.4. Lwm yam khoom siv lom neeg

Txij li thaum Thanaka bark hmoov tau ib txwm siv los tiv thaiv UV thiab kho tawv nqaij los ntawm Burmese, nws txhawb cov kws tshawb fawb los ntsuas nws cov tyrosinase inhibition kev ua haujlwm. Tyrosinase yog tooj liab-muaj enzyme uas tau lees paub rau melanogenesis thiab pigmentation kev ua [25]. Yog li, tyrosinase inhibition kev ua haujlwm tseem ceeb los tiv thaiv kev tsim cov xim ntawm daim tawv nqaij. Wangthong et al. (2010) soj ntsuam tyrosinase inhibition kev ua ntawm Thanaka qia bark extracts. Tag nrho cov tyrosinase inhibition kev ua ntawm Thanaka qia bark extracts thiab ntshiab hmoov yog me me thaum piv nrog tus qauv Kojic acid; Txawm li cas los xij, thaum piv nrog ntau yam Thanaka extracts,

Cov tshuaj tiv thaiv kab mob ntawm Thanaka qia bark extracts kuj tau soj ntsuam los ntawm Wangthong li al. (2010). Kev mob yog ib qho qauv ntawm lub cev rau kev raug mob los yog kev ua xua uas cuam tshuam nrog kev sib txuam ntawm cov hlwb thiab exudates hauv cov ntaub so ntswg los tiv thaiv thiab tiv thaiv kev puas tsuaj ntxiv. Txawm li cas los xij, cov tshuaj tiv thaiv kab mob ua rau mob, liab, o, thiab kub rau lub cev; Yog li ntawd, lub luag haujlwm ntawm kev tiv thaiv kab mob yog qhov tseem ceeb hauv kev txo cov tsos mob no. Kev ua haujlwm tiv thaiv kab mob ntawm ntau yam Thanaka qia bark extracts tau ua nyob rau hauv murine macrophage-zoo li cell kab RAW 264.7 siv cov qia bark rho tawm thiab lawv pom tias tag nrho cov extracts muaj 80-90 feem pua ​​​​siab tiv thaiv kev ua haujlwm ntawm cov tshuaj tsis muaj tshuaj lom. (80 feem pua ​​​​ntawm tes viability) nyob rau hauv qhov kev txiav txim: Hexane > dichloromethane > ethyl acetate > 85 feem pua ​​​​ethanol > methanol > dej. Li no, nws tau raug pov thawj tias cov qia bark extracts ntawm Thanaka muaj kev tiv thaiv kev ua haujlwm siab thiab qib me me ntawm tyrosinase inhibition kev ua haujlwm [18].

Vasant thiab Narasimahcharya (2013) tau sim nrog lub peev xwm ntawm roj av ether extract los ntawm Thanaka txiv hmab txiv ntoo hmoov cov kev cai fluoride-induced hyperglycemia thiab hyperlipidemia nyob rau hauv colony-bred txiv neej albino nas. Hyperglycemia yog qhov mob ntawm cov ntshav qabzib ntau hauv cov ntshav thiab thaum muaj kev pheej hmoo siab, yuav ua rau mob ntshav qab zib thaum hyperlipidemia yog qhov ua rau nce cov roj cholesterol lossis triglycerides hauv cov ntshav, ob qho tib si ua rau mob hnyav. Hauv cov pab pawg nas noj nrog Thanaka txiv hmab txiv ntoo hmoov (2.5 g / kg hauv pub, 5 g / kg hauv pub, 10 g / kg infeed), nws pom cov koob tshuaj ntawm cov txiaj ntsig tseem ceeb hauv kev txo qis ntawm cov piam thaj hauv plasma, G{{8} Kev ua haujlwm Pase, plasma tag nrho lipid (TL), tag nrho cov roj cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), very-low-density lipoprotein cholesterol (VLDL-C), apolipoprotein (AI) Cov ntsiab lus thiab cov kab mob siab lipid profile, thaum nce hauv cov ntshav ntshav siab lipoprotein cholesterol (HDL-C) cov ntsiab lus kuj tau pom [15]. Yog li, cov roj av ether extract ntawm Thanaka txiv hmab txiv ntoo extract tuaj yeem tswj hwm hyperglycemia thiab hyperlipidemia.

Lub qhov txhab kho qhov txhab ntawm Thanaka nplooj biomolecules txheej nyiaj nanoparticles (AgNPs) kuj tau soj ntsuam los ntawm Bhuvaneswari li al. (2014) ntawm Wistar txiv neej albino nas thiab piv nrog cov qauv tshuaj Betadine [24]. Silver nanoparticles yog cov tshuaj tiv thaiv kab mob uas tau siv rau hauv cov tshuaj pleev rau ntawm daim tawv nqaij thiab cov tshuaj pleev kom xa cov ntawv siv dav los tiv thaiv kev kis kab mob thiab qhib qhov txhab [26]. Cov txiaj ntsig tau pom tias Thanaka nplooj AgNPs muaj kev ua kom lub qhov txhab zoo dua li cov qauv tshuaj betadine [24]. Txawm li cas los xij, cov kws sau ntawv tsis tau muab piv rau Thanaka nplooj AgNPs kho cov nyhuv nrog tsuas yog Thanaka nplooj extract; Yog li ntawd, tsis muaj kev txiav txim siab yuav ua rau seb qhov kev kho qhov txhab puas yog vim yog cov nyiaj nanoparticles lossis yog Thanaka extract boosted cov nyhuv kho ntawm AgNPs los yog ob qho tib si.

5. Cov khoom siv tshuaj pleev ib ce uas muaj Thanaka nyob rau sab hnub tuaj Asia Market

Hauv Table 4, peb tau txheeb xyuas cov khoom siv tshuaj pleev ib ce Thanaka uas tau tsim thiab muag hauv cov tebchaws Esxias sab hnub tuaj xws li Myanmar, Thaib, thiab Malaysia. Cov npe tseem ceeb, uas yog, Shwe Pyi Nann [27] thiab Tseeb Thanaka [28] los ntawm Myanmar, Suppaporn [29] thiab De Leaf [30] los ntawm Thaib teb, Thanaka Malaysia [31], thiab Bio Essence [32] los ntawm Malaysia feem ntau pom. hla lub txee hauv khw muag khoom thiab khw muag tshuaj thiab hauv online. Cov khoom lag luam hauv zos los ntawm Myanmar (snake hom, Pann Chit Thu, Myat Bhoon Pwint), thiab Malaysia (Taté Skincare Malaysia [33]) kuj yog cov tsim khoom ntawm Thanaka cosmeceutical khoom.

Shwe Pyi Nann Co. Ltd. yog cov chaw tsim khoom thiab xa tawm Thanaka mus rau Thaib teb, Malaysia, Singapore, thiab Philippines, uas ua rau kev tsim cov khoom Thanaka hauv Thaib teb thiab Malaysia. Cov tuam txhab tsim cov khoom lag luam Thanaka feem ntau pom nyob rau sab hnub tuaj Asia txij li Thanaka tawv tawv zoo nyob rau hauv huab cua kub ntawm cov teb chaws nyob ze ntawm txoj kab nruab nrab, qhov chaw tshav ntuj yog qhov hnyav dua piv rau lwm qhov hauv ntiaj teb. Yog li, txhawm rau tiv thaiv peb cov tawv nqaij los ntawm qhov teeb meem UV rays, qhov kev thov rau cov khoom thaiv hnub ci thiab kev kho mob tom qab tshav ntuj yog qhov tseem ceeb hauv cov tebchaws Esxias sab hnub tuaj.

Cov neeg Burmese siv Thanaka hmoov ncaj qha rau ntawm lawv daim tawv nqaij ua tshuaj pleev thaiv hnub. Txawm li cas los xij, thaj tsam daj sab laug ntawm sab plhu (Daim duab 1) tsis tau txais los ntawm lwm lub tebchaws tsuas yog Myanmar. Yog li ntawd, kom tau txais txiaj ntsig zoo rau tib neeg nrog tshuaj pleev thaiv hnub ntuj, Thanaka cov khoom tu tawv nqaij xws li xab npum, cov hmoov xoob, cov hmoov hauv paus, tshuaj pleev ntsej muag, pleev lub cev, thiab tshuaj pleev ntsej muag yog tsim. Txhawm rau ua kom tau raws li cov neeg siv khoom thiab cov kev xav tau ntawm kev lag luam, Thanaka kuj tau tsim los rau hauv cov tshuaj ntxuav tes, tshuaj pleev xim, tshuaj pleev xim, tshuaj pleev xim pob txuv, thiab cov tshuaj pleev kom zoo nkauj. Cov tuam txhab lag luam feem ntau ntxiv cov khoom xyaw nquag xws li cov vitamins, collagen, thiab hyaluronic acid los ua kom cov nyhuv synergic thiab muab kev kho mob rau ntau yam tawv nqaij. Qee cov khoom lag luam ntawm Shwe Pyi Nannare tau txhim kho nrog cov ntxhiab tsw ntawm paj thiab tshuaj ntsuab kom cov khoom zoo nkauj zoo nkauj rau cov neeg siv khoom. Feem ntau, ib qho ntxhiab tsw ntxiv rau cov khoom zoo nkauj kom tshem tawm cov ntxhiab tsw ntawm nws cov khoom xyaw. Hom xws li Truly Thanaka, de Leaf, thiab ThanakaMalaysia tsim cov khoom Thanaka uas muaj cov vitamins A, C, thiab E, collagens, 24 k kub, hyaluronic acid, aloe vera, turmeric, glutathione, jasmine mov, thiab pomegranate hmoov uas muaj txiaj ntsig zoo rau daim tawv nqaij. , xws li tiv thaiv peb cov tawv nqaij los ntawm ib puag ncig kev puas tsuaj xws li kev ua qias tuaj, txhim kho peb cov tawv nqaij mob thiab pab tiv thaiv cov teebmeem ntawm kev laus xws li wrinkles thiab pigmentation. Tsis tas li ntawd, cov khoom xyaw zoo xws li xyoob charcoal tau ntxiv los ua ib qho exfoliator, lub sijhawm no, kaffir txiv qaub thiab zib ntab tau ntxiv los txhim kho cov nyhuv ntxuav thiab lub cev ci.

6. Cov lus xaus thiab kev pom

Thanaka tau siv los ua cov tshuaj pleev ib ce los ntawm cov neeg Myanmar rau ntau tshaj 2000 xyoo vim kev ntseeg ntawm nws txoj kev tiv thaiv kev laus, pob txuv-tshuaj, thiab kev tiv thaiv tshav ntuj. Cov neeg nyob rau sab hnub tuaj Asia kuj siv nws los ua ib qho tshuaj kho mob rau ntau lub hom phiaj xws li tshuaj tua kab thiab kho qhov txhab. Ntawm nws cov txiaj ntsig muaj npe nrov, nws cov tshuaj tua kab mob antioxidant, tshuaj tua kab mob, thiab cytotoxicity yog cov khoom siv feem ntau kawm siv ntau yam Thanaka extracts. Kev tshuaj xyuas phytochemical yog ib txoj hauv kev nyiam los ntawm cov kws tshawb fawb los tshawb pom lub ntuj bioactive tebchaw hauv Thanaka.

Txawm hais tias qhov kev tshuaj ntsuam chromatography ntawm Thanaka tau ua tiav ntawm kev rho tawm ntawm ntau cov kuab tshuaj, cov kws sau ntawv tsis tau tham ntxiv txog kev ua haujlwm lom neeg ntawm txhua qhov sib xyaw ua ke hauv feem ntau ntawm cov ntawv tshaj tawm tau tshuaj xyuas hauv Table 2. Hloov pauv, feem ntau ntawm cov tshuaj lom neeg tau ua los ntawm kev siv cov extracts ntawm Thanaka. tsis muaj kev sib cais ntxiv ntawm cov ntshiab bioactive tebchaw los ntawm cov extracts (Table 3). Qhov sib txawv no tuaj yeem txhim kho los ntawm kev sib koom tes ntawm chemists thiab biologists hauv kev tshawb pom ntawm bioactive compounds hauv cov khoom ntuj. Ntxiv mus, feem ntau cov kws sau ntawv siv cov kuab tshuaj organic xws li hexane, chloroform, ethyl acetate, ethanol, thiab methanol (Table 3) los ua qhov kev rho tawm. Wangthong et al. (2010) tau hais tias solubility ntawm extracts thiab toxicity ntawm cov organic solvents yuav cuam tshuam qhov tseeb ntawm cov txiaj ntsig raws li feem ntau ntawm cov tshuaj lom neeg siv cov kev daws teeb meem polar buffer [18]. Yog li, kev siv cov kuab tshuaj ntsuab (xws li glycerol) hauv kev rho tawm cov khoom xyaw bioactive tej zaum yuav yog lwm txoj hauv kev zoo rau cov kuab tshuaj organic hauv kev rho tawm ntawm cov khoom ntuj, tshwj xeeb, hauv kev tsim cov khoom siv tawv nqaij. Kev txhim kho ntawm cov khoom tu tawv nqaij ntsuab yuav tiv thaiv cov neeg siv kom tsis txhob muaj kev tsis haum rau ntawm daim tawv nqaij. Tsis tas li ntawd, cov kuab tshuaj ntsuab xav tau kev tswj cov khib nyiab tsawg kawg nkaus. Nws cia siab tias qhov kev tshuaj xyuas no tuaj yeem ua ib qho kev siv uas yuav ua rau muaj kev tshawb pom tshiab.

Tus sau kev koom tes:Conceptualization, MWL, LTG thiab MKA; methodology, LTG; thawj daim ntawv npaj ntawm cov ntawv sau, MWL thiab LTG; tshuaj xyuas thiab kho cov ntawv sau, LTG thiab MKA; kev saib xyuas, LTG; Kev nrhiav nyiaj txiag, LTG thiab MKA Txhua tus kws sau ntawv tau nyeem thiab pom zoo rau cov ntawv luam tawm ntawm cov ntawv sau.

Nyiaj txiag: Qhov kev tshawb fawb no tau txais nyiaj los ntawm Fundamental Research Grant Scheme (FRGS) (Grant no.: FRGS/1/2020/STG01/SYUC/02/1) muab los ntawm Ministry of Education Malaysia (MOE).

Institutional Review Board Statement:Tsis siv tau.

Cov Lus Qhia Txog Kev Pom Zoo:Tsis siv tau.

Cov ntaub ntawv muaj nyob:Tsis siv tau.

Kev lees paub: Cov kws sau ntawv xav lees paub Sunway Postgraduate Research Scholarship (PGSUREC2020/006) uas tau tso cai rau MW Lim los ntawm Sunway University, Malaysia.

Kev tsis sib haum xeeb ntawm kev txaus siab:Cov kws sau ntawv tshaj tawm tias tsis muaj kev cuam tshuam ntawm kev txaus siab.

Cov ntaub ntawv

1. Yeni. Kev zoo nkauj uas yog ntau tshaj daim tawv nqaij sib sib zog nqus. Xyoo 2011.

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27. Thanaka Shop.

28. Tiag Tiag.

29. Supaporn Group.

30. De Nplooj.

31. Thanaka Malaysia.

32. Bio essence.

33. Taté Skincare Malaysia.

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