Kawm Txog Kev Ua Haujlwm Arginase Thiab Nws Qhov Kev Sib Raug Zoo Nrog Lub Siab Thiab Lub raum Ua Haujlwm Rau Cov Neeg Mob Hom -2 Ntshav Qab Zib Mellitus hauv Nineveh
Mar 15, 2022
Hu rau: ali.ma@wecistanche.com
Ruaa Waleed Alramadhany, Hamodat, Zahraa Mohammed Al
Abstract
Keeb kwm:Arginaseplays lub luag haujlwm tseem ceeb hauv hom 2 mob ntshav qab zib mellitus. Lub hom phiaj ntawm txoj kev tshawb no: Txoj kev tshawb no tsom los txiav txim qhov kev ua ntawmarginaseHauv cov neeg mob ntshav qab zib mellitus hom 2 (DMT2) thiab kawm txog kev sib raug zoo ntawmarginaseKev ua si thiab cov kev tshawb xyuas qhov sib txawv Cov neeg mob thiab cov khoom siv: Txoj kev tshawb no suav nrog 201 cov neeg laus muab faib ua ob pawg: cov neeg uas muaj DMT2 (136 tus neeg) thiab pawg tswj hwm (65 tus neeg). Cov ntshav uas tsis yoo mov tau txiav txim siabarginaseKev ua haujlwm, qabzib, glycated hemoglobin (HbA1c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, thiab creatinine qib. Cov txiaj ntsig: Cov kev tshawb pom pom tias qib ntawmarginasekev ua haujlwm hauv cov neeg mob nrog DMT2 tau nce ntxiv (p Tsawg dua lossis sib npaug rau 0.0001) hauv kev sib piv nrog cov pab pawg tswj hwm, tab sis tsis muaj qhov sib txawv tseem ceeb hauv kev ua ntawmarginaserau ob leeg poj niam txiv neej thiab hnub nyoog. Kev sib raug zoo tseem ceeb (P=0.01) tau pom ntawm Glucose (r=0.584), HbA1c (r=0.454), AST(GOT) (r{{7 }}.433), ALT (GPT) (r=0.295), ALP (r=0.639), Urea (r=0.667), thiab Creatinine (r{ {15}}.658). Xaus: Hauv cov neeg mob nrog DMT2, nce kev ua haujlwm ntawmarginaseplays lub luag haujlwm tseem ceeb los ntawm nws yog ncaj qha cuam tshuam rau daim siab thiablub raum ua haujlwm.
Ntsiab lus:Arginase, ntshav qab zib mellitus hom 2, Glucose, HbA1c feem pua.
Nyem rau cistanche hmoov noj qab haus huv pab rau lub raum
1. Taw qhia
Mob ntshav qab zib mellitus yog suav tias yog ib qho ntawm cov kab mob uas ua rau tuag thaum tsis muaj kev soj ntsuam ntawm tus kab mob, vim nws tau pom tias ntshav qab zib nce ntxiv, thiab hauv kev tshawb fawb uas tau sau tseg hauv 2017, kwv yees li 462 lab tus tib neeg thoob ntiaj teb tau cuam tshuam. los ntawm hom 2 mob ntshav qab zib, suav txog 6.28 feem pua ntawm cov neeg thoob ntiaj teb (4.4 feem pua ntawm cov hnub nyoog 15-49 xyoo, 15 feem pua ntawm cov hnub nyoog 50-69 xyoo, thiab 22 feem pua ntawm cov hnub nyoog tshaj 70 xyoo) [1].
Tsis tas li ntawd, ntshav qab zib mellitus muaj ntau hom, qhov tseem ceeb tshaj plaws yog hom 1, hu ua insulin-dependent Juvenile Diabetes. Hom thib ob, uas yog hu ua cov neeg mob ntshav qab zib mellitus, tsis yog nyob ntawm insulin [2]. Arginase (EC 3.5.3.1) catalyzes L-arginine hydrolysis rau hauv L-ornithine nrog rau urea thiab xav tau rau nws cov tshuaj tiv thaiv cofactor, tshwj xeeb tshaj yog Mn plus 2. Arginase yog ib feem ntawm lub voj voog urea uas tswj cov qib L-arginine [3] . Arginase yog ib qho tseem ceeb enzymes koom nrog hauv cov metabolism thiab tsim ntawm urea [4,5]. Arginase muaj ob isozymes, thawj yog pom nyob rau hauv cytosol nyob rau hauv lub siab, uas plays lub luag hauj lwm tseem ceeb nyob rau hauv lub urea voj voog, thiab lub thib ob yog nyob rau hauv lub mitochondria ntawm lub mitochondria.lub raumthiab prostate [4,6].
Tsis tas li ntawd, ib txoj kev tshawb fawb tau pom tias cov neeg mob nrog DMT2 muaj kev nce hauv cov haujlwm ntawm arginase [7], thiab hauv lwm cov kev tshawb fawb tau ua rau cov nas raug mob ntshav qab zib, lawv muaj kev nce hauv cov haujlwm ntawm arginase. Nws kuj tau pom tias cov neeg mob DMT2 muaj qhov ua tsis tiav hauv daim siab ua haujlwm thiab nce kev ua haujlwm ntawm txhua AST, ALT, thiab ALP [8,9]. Tsis tas li ntawd, Nws kuj tau pom tias cov neeg mob ntshav qab zib tau nce siab hauv glycated hemoglobin, tshwj xeeb tshaj yog cov neeg mob tsis kho, piv rau cov neeg mob kho thiab qhov sib txawv ntawm daim siab ua haujlwm. Tsis tas li ntawd, DMT2 raug kev txom nyem los ntawmimpaired raum ua haujlwmlos ntawm qib siab ntawm urea thiab creatinine [10,11,12].
Peb txoj kev tshawb fawb tsom los ntsuas qhov kev ua ntawm arginase hauv sera ntawm cov neeg mob nrog DMT2 thiab nws yog kev sib raug zoo rau lub siab thiablub raum ua haujlwm.
2. Cov ntaub ntawv thiab cov txheej txheem
2.1.population study:
Txoj kev tshawb no tau ua rau 201 cov neeg mob uas tau muab faib ua ob pawg, thawj ntawm 136 tus neeg mob ntshav qab zib hom 2 ntawm ob leeg poj niam (78) poj niam (58) txiv neej, thiab lawv cov hnub nyoog sib txawv ntawm (35-75). Cov pab pawg noj qab haus huv suav nrog 65 tus neeg tsis muaj ntshav qab zib thiab lwm yam kab mob ntawm ob leeg poj niam txiv neej (33) poj niam (32) txiv neej; lawv cov hnub nyoog muaj nyob ntawm (35-68). Cov neeg mob ntshav qab zib muaj lwm yam kab mob xws li lub plawv, mob qog noj ntshav, thiabmob raumraug cais tawm.
Txoj kev tshawb no tau ua los ntawm cov neeg mob ntshav qab zib uas tuaj xyuas cov chaw kho mob ntawm Al-Wafa Center thiab cov chaw kho mob sab nraud uas tau tso cai los ntawm Nineveh Health Department. Cov neeg mob tau muab faib ua ob pawg raws li hnub nyoog, pawg thawj yog (35-45) xyoo, thiab pawg thib ob yog (Ntau dua lossis sib npaug li 46).
Tsis tas li ntawd, Pawg Saib Xyuas Kev Ncaj Ncees ntawm University of Mosul (College of Science) thiab Nineveh Health Department ua raws li txoj cai kawm. Txhua tus neeg tuaj koom tau muab ib daim ntawv nug kev pom zoo los koom nrog hauv kev tshawb fawb.
2.2. Cov Qauv Sau:
2.2.1. Cov qauv ntshav tau txais los ntawm kev nqus 5 ml ntawm cov ntshav venous, tom qab ntawd muab tso rau hauv ib lub raj gel thiab tawm rau 15 feeb ntawm qhov kub ntawm 37Ċ thiab centrifuging 3000xg, ces cais cov ntshav thiab khaws cia ntawm qhov kub ntawm -20 Ċ kom txog thaum qhov kev xeem tsim nyog tau ua tiav.
2.2.2. Tag nrho cov ntshav 2 ml ntawm cov ntshav venous raug rho tawm tsuas yog cov neeg mob ntshav qab zib mellitus hom 2 los ua ib qho kev kuaj glycated hemoglobin thiab muab tso rau hauv cov hlab uas muaj cov tshuaj tiv thaiv coagulant EDTA.
2.3. Variables Assay
2.3.1 Kev kwv yees ntawm arginase kev ua haujlwmArginase tau ntsuas los ntawm kev ua raws li kev hloov kho [13]. Qhov kev ua ntawm arginase sawv cev rau cov enzyme xav tau los hloov micromol ntawm arginine mus rau ornithine ib feeb.
2.3.2 Kev ntsuas glycated hemoglobin
glycated hemoglobin tau ntsuas los ntawm German-ua On-Call ntaus ntawv, siv lub tuam txhab cov khoom siv G136-111.
2.3.3 Kev ntsuas cov piam thaj hauv cov ntshav qabzib concentration tau ntsuas los ntawm txoj kev colorimetric enzymatic, siv cov khoom tsim los ntawm Randox GL364 [13].
2.3.4 Ntsuas cov dej num ntawm ALT, AST, thiab ALP. Siv cov Japanese-keeb kwm Fuji automated analyzer ntaus ntawv thiab ua raws li cov chaw tsim tshuaj paus cov lus qhia
2.3.5 Kev ntsuas qhov concentration ntawm urea thiab creatinine concentrations ntawm urea tau ntsuas siv cov khoom siv los ntawm biosystems tuam txhab [15] thiab creatinine yog ntsuas los ntawm cov khoom siv los ntawm Biolabo Company [16].
2.4.Kev txheeb xyuas qhov tseeb
Kev tshuaj xyuas tau ua tiav siv SPSS version 25. Cov ntaub ntawv raug tshuaj xyuas. Qhov nruab nrab ± tus qauv sib txawv (SD) tau txais; ob pawg tau muab piv nrog kev ywj pheej T-test. Pearson correlation coefficient yog siv los tshawb txog kev sib raug zoo ntawm cov ntshav arginase kev ua haujlwm nrog cov kev tshawb fawb qhov sib txawv. Cov 0.0001 P-tus nqi tau muab coj los rau hauv tus lej suav nrog qhov tseem ceeb[17].
3. Cov txiaj ntsig thiab kev sib tham
Mob ntshav qab zib mellitus Hom 2 (DMT2) yog ib yam kab mob uas tshwm sim, thiab tsis tuaj yeem saib xyuas thiab kho qhov tsim nyog yuav ua rau tuag [18,19]. DMT2 ua rau muaj kev tsis txaus ntseeg hauv lub cev ua haujlwm, xws li kev tsis txaus ntseeg hauv kev ua haujlwm ntawm lub siab thiab lub raum [20]. Thiab nyob rau hauv lem, nws cuam tshuam rau tus so ntawm lub cev ua hauj lwm raws li nws muaj feem xyuam rau cov enzymes xws li arginase [21,22,23]. Yog li ntawd, hauv txoj kev tshawb no, peb tau txiav txim siab los kawm txog kev ua tau zoo ntawm arginase kev ua haujlwm thiab nws qhov kev sib raug zoo nrog cov kev tshawb fawb sib txawv, suav nrog cov piam thaj, glycated hemoglobin (HbA1c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, thiab creatinine.
Table 1 qhia txog cov yam ntxwv ntawm kev kawm pej xeem; qhov twg qhov nruab nrab ± SD hnub nyoog ntawm pawg tswj hwm thiab cov neeg mob DMT2 yog 48.3 ± 10.6 xyoo thiab 50.7 ± 10.3 xyoo, raws li, thiab qhov feem pua ntawm cov poj niam (feem pua 57.4) cuam tshuam los ntawm tus kab mob ntau dua li txiv neej (feem pua 42.6) kev ua haujlwm Qhov siab thiab qhov kev txhaj tshuaj insulin tuaj yeem txo nws [24]. Txo cov substrate concentration yog lwm yam tseem ceeb uas yuav piav qhia txog kev txo qis nitric oxide (NO) tiam. Arginase's accelerated enzymatic hloov ntawm arginine rau ornithine. Arginase yog zoo ib yam nrog no nitrogen oxide hom NOS vasodilatory zog txo. [24, 26].
Qee yam cuam tshuam rau kev ua arginase kuj tau kawm, suav nrog poj niam txiv neej thiab hnub nyoog hauv cov neeg mob thiab pawg tswj hwm. Table (2) thiab Daim duab 1 qhia tias qhov sib deev yam tsis muaj kev cuam tshuam rau kev ua haujlwm ntawm arginase enzyme hauv cov neeg mob thiab pab pawg tswj. Qhov kev ua ntawm arginase yog siab dua rau cov txiv neej tshaj li poj niam hauv txhua tus neeg mob thiab Pab Pawg Tswjhwm, tab sis tsis muaj qhov sib txawv tseem ceeb, tsis muaj qhov cuam tshuam ntawm lub hnub nyoog ntawm kev ua haujlwm ntawm arginase hauv cov neeg mob thiab pab pawg tswj. Txawm li cas los xij, kev ua ntawm arginase nce nrog lub hnub nyoog tab sis tsis muaj qhov sib txawv loj.
Table (3) piv cov kev tshawb fawb sib txawv, suav nrog cov piam thaj, glycated hemoglobin, lub siab thiab lub raum ua haujlwm hauv cov neeg mob DMT2 thiab lawv qhov kev sib piv nrog kev tswj hwm. Ib qho tseem ceeb nce piv nrog rau pawg tswj hwm. Ib qho kev nce ntxiv (p Tsawg dua lossis sib npaug rau 0.0001) hauv cov piam thaj concentration rau cov neeg mob piv rau pawg tswj hwm pom zoo nrog (Kashyap et al .2008) [24]. Tsis tas li ntawd, nce HbA1c feem pua pom zoo nrog (shatanawi li al. 2017)[24,26,27].
Cov kev tshawb fawb tau pom tias muaj qhov nce ntxiv (p Tsawg dua lossis sib npaug rau 0.0001) hauv AST, ALT, thiab ALP hauv cov neeg mob DMT2 piv rau pawg tswj hwm, thiab qhov no zoo ib yam nrog [28,29 ]. Txoj kev tshawb no yog piv rau txoj kev tshawb fawb los ntawm Salman et al [29] thiab ob qhov kev tshawb fawb ntxiv ua los ntawm Idris et [30], uas tau tshaj tawm qhov pom tseeb ntawm ALT thiab AST. Nim et al. Htet kuj tau tshawb fawb txog cov neeg mob ntshav qab zib hauv Malaysia, thiab kev tshawb fawb pom tias cov neeg mob no tau kuaj lub siab ua haujlwm tsis zoo [31]. Txhawm rau ntxiv, Wang et al. tau ua txoj kev tshawb fawb uas lawv tau tshuaj xyuas cov neeg mob DMT2 thiab tswj. Lawv cov kev tshawb fawb pom tau tias ALT muaj feem cuam tshuam nrog kev pheej hmoo siab mob ntshav qab zib hom 2 hauv cov neeg Suav [32]. Txoj kev tshawb no pom tias ALT tau cuam tshuam nrog kev pheej hmoo ntawm DMT2 hauv Suav cov neeg. Qhov tshwm sim ntawm cov enzymes no tuaj yeem ua rau muaj qhov cuam tshuam loj rau hepatotoxic mob siab rau insulin tsis kam (kuaj raws li insulin tsis kam lossis HbA1c) tuaj yeem ua rau muaj cov roj fatty acids ntau dua (piv txwv li, fatty acids dawb) hauv siab [28,33]. Qhov kev txuam no tuaj yeem yog vim muaj kev cuam tshuam ntawm cov cell membrane, ua haujlwm tsis zoo ntawm mitochondria, thiab nce ntau cov khoom xws li transaminases, fatty acids, thiab tshuaj lom metabolites. Cov kev cuam tshuam no kuj tseem tuaj yeem ua rau mitochondrial tsis ua haujlwm, oxidative kev nyuaj siab, thiab nce hauv pro-inflammatory cytokines [34,29].
Cov txiaj ntsig kuj tau pom nyob rau hauv Table 3 qhov cuam tshuam ntawm arginase kev ua haujlwm ntawm lub raum ua haujlwm, uas suav nrog urea thiab creatinine, uas cov txiaj ntsig tau pom qhov nce ntxiv (p Tsawg dua lossis sib npaug rau 0.0001) rau txhua qhov ntawm urea, creatinine hauv cov neeg mob nrog DMT2 piv rau pawg tswj hwm, qhov no zoo ib yam nrog lwm cov kev tshawb fawb [35,36,37,38].
Raws li txoj kev tshawb no, qhov siab ntawm urea thiab creatinine hauv cov ntshav ntawm cov neeg mob ntshav qab zib tau qhia tias muaj kab mob ua ntej lub raum [36]. Hauv 2017, Abdul Rahman Al-Dakhil tau tshawb fawb hauv Saudi Arabia uas qhia txog kev sib piv [39]. Iraq muaj ntau tshaj plaws ntawm cov ntshav qab zib nephropathy ntawm cov tebchaws Arab, thaum lub tebchaws United Arab Emirates muaj qhov qis tshaj plaws hauv Bahrain [39].
Kev sib raug zoo ntawm arginase kev ua haujlwm nrog kev kawm sib txawvDaim duab 4 thiab daim duab 2 qhia txog kev sib raug zoo ntawm arginase kev ua haujlwm tau kawm nrog cov kev hloov pauv, uas suav nrog cov piam thaj, HbAc feem pua, AST, ALT, ALP, urea, thiab creatinine.
1. Kev sib raug zoo ntawm arginase kev ua si nrog cov txiaj ntsig ntawm qabzib tau pom nyob rau hauv Table (4) thiab daim duab (2,1) tias muaj kev sib raug zoo ntawm kev ua haujlwm ntawm arginase thiab cov concentration ntawm cov piam thaj hauv cov ntshav. Cov txiaj ntsig no pom zoo nrog [40]. Thaum peb txoj kev tshawb fawb tsis pom zoo nrog Shatanawi thiab Momani, 2017. Qhov twg lawv pom tias cov qib ntawm arginase tsis qhia txog kev sib raug zoo nrog cov concentration ntawm cov ntshav qab zib [25].
2-kev sib raug zoo ntawm arginase thiab HbA1cCov txiaj ntsig tau pom hauv Table (4) thiab Daim duab (2, 2) tias muaj kev sib raug zoo ntawm kev ua haujlwm arginase thiab HbA1c. Qhov no yog raws li kev tshawb fawb yav dhau los [25]. Qhov ntawd pom muaj kev sib raug zoo ntawm kev ua haujlwm ntawm arginase thiab HbA1c vim tias qhov kev cuam tshuam ntev rau cov piam thaj ntau yog tsim nyog los ua kom cov haujlwm ntawm arginase.
3-kev sib raug zoo ntawm arginase thiab lub siab ua haujlwmCov txiaj ntsig tau pom nyob rau hauv Table (4) thiab Daim duab (2.3), (2.4) thiab (2.5) ib qho kev nce ntxiv thiab kev sib raug zoo ntawm kev ua haujlwm ntawm arginase thiab daim siab ua haujlwm hauv cov neeg mob DMT2 piv rau pawg tswj hwm AST, ALT, thiab ALP. Qhov kev tshwm sim no zoo ib yam nrog kev tshawb nrhiav pom tias cov txiaj ntsig ntawm kev kuaj mob siab rau cov neeg mob DMT2 tau nce siab dua piv rau pawg tswj hwm [41].
Qhov kev sib raug zoo ntawm arginase kev ua haujlwm nrog cov enzymes no yog vim muaj cov tshuaj lom hauv lub siab vim yog cov fatty acids uas tau tsim nyob rau hauv ntau ntau ntawm daim siab hlwb vim yog insulin tsis kam [33].
3-Kev sib raug zoo ntawm arginase thiab lub raum ua haujlwm urea thiab creatinineCov txiaj ntsig tau pom nyob rau hauv Table (4) thiab Daim duab (2.6), (2.7) qhov kev sib raug zoo tseem ceeb ntawm kev ua haujlwm ntawm arginase thiab lub raum ua haujlwm hauv cov neeg mob DMT2. Kev tshawb nrhiav pom tias muaj kev pom zoo nrog [42]. Cov kev tshawb pom ntawm qhov kev tshawb fawb no kuj tau qhia tias kev tswj cov ntshav qab zib tsis zoo tuaj yeem ua rau cov ntshav qabzib siab thiab cov ntshav urea, ua rau cov neeg mob ntshav qab zib muaj kev pheej hmoo siab dua los ntawm kev tsim nephropathy. Qhov no yog nyob rau hauv txoj kab nrog kev tshawb pom ntawm kev tshawb fawb ua ntej, uas pom tau tias feem ntau ua rau mob raum raug mob yog hyperglycemia [36].
Cov lus xaus
Kev nce ntxiv ntawm arginase hauv cov neeg mob nrog DMT2 ncaj qha cuam tshuam rau lub siab thiab lub raum ua haujlwm. Tsis tas li ntawd, kev ua haujlwm tsis zoo hauv daim siab thiab lub raum ua haujlwm feem ntau tshwm sim nrog DMT2 ntshav qab zib hauv Nineveh Governorate. Yog li ntawd, kev tshuaj xyuas rau daim siab thiab lub raum tsis ua haujlwm hauv cov neeg mob ntshav qab zib yuav tsum tau tiv thaiv kom tsis txhob muaj teeb meem ntxiv nrog rau lub siab thiab lub raum.
Kev lees paub
Kuv ua tsaug thiab txaus siab rau kuv cov tsev kawm qib siab deanship (College of Science) thiab kuv chav haujlwm (Chemistry). Raws li Nineveh Lub Tsev Haujlwm Saib Xyuas Kev Noj Qab Haus Huv los pab txhawb kev coj ua ntawm txoj kev tshawb fawb no.
Nyiaj txiagTxoj kev tshawb no tsis tau txais nyiaj txiag sab nraud.
Cov ntaub ntawv
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