Liang Qingsong 1,2,4, Pan Yingjie 2,4, Chen Ziying 2,4, Zhang Ying 1,3, Wu Menghua 1,3, Cao Hui 1,3, Ma Zhiguo 1,3 *

(1. Tsev Kawm Ntawv Pharmacy, Jinan University, Guangzhou 510632, Guangdong; 2. Guangdong Yifang Pharmaceutical Co., Ltd., Foshan 528244, Guangdong; 3. Lingnan Traditional Chinese Medicine Research Center, Jinan University, Guangzhou 510632, Guangd Lub Tuam Txhab Tseem Ceeb ntawm Guangdong Traditional suav tshuaj Formula Granules Enterprise, Foshan 528244, Guangdong)

Abstract: Lub Hom Phiaj Los npaj cistanche deserticola txheem decoction thiab muab ib txoj kev txhim khu kev qha rau nws cov kev tswj kom zoo. Txoj kev Raws li kev npaj yuav tsum tau ntawm tus qauv decoction, 14 batch ntawm Cistanche deserticola txheem decoction tau npaj. Cov ntsiab lus ntawm echinaside thiab piloside hauv tus qauv decoction tau txiav txim siab los ntawm HPLC, tus nqi hloov pauv tau suav, tus nqi rho tawm thiab pH tus nqi ntawm cov decoction raug ntsuas, thiab UPLC ntiv tes ntawm Cistanche deserticola tus qauv decoction tau tsim. Cov txiaj ntsig Qhov nruab nrab ntawm kev hloov pauv ntawm echinaside thiab piloside hauv 14 pawg ntawm Cistanche deserticola tus qauv decoction yog 22.99 feem pua ​​thiab 17.{20}}9 feem pua. Tus nqi rho tawm txawv ntawm 25.1 feem pua ​​​​rau 56.6 feem pua, thiab tus nqi pH txawv ntawm 4.30 txog 5.15. Muaj 6 qhov siab tshaj plaws hauv cov ntiv tes, thiab ntawm lawv, No. 1 ncov tau txheeb xyuas tias yog echinacea glycoside, No. 3 ncov tau txheeb xyuas tias yog angiocyanin A, No. 4 ncov tau txheeb xyuas tias yog piloside, thiab No. 5. ncov yog txheeb xyuas raws li isopiloside. Qhov zoo sib xws ntawm 14 pawg ntawm Cistanche deserticola tus qauv decoction tau soj ntsuam, thiab qhov zoo sib xws ntawm 0.789 txog 0.999. Xaus Kev npaj ntawm Cistanche deserticola standard decoction yog standardized, nrog tag nrho cov homogeneity zoo, controllable zoo, zoo raug, stability, thiab repeatability ntawm txoj kev txiav txim, thiab siab ntiv tes zoo sib xws, uas muaj peev xwm muab ib tug siv rau kev tswj zoo ntawm Cistanche deserticola txheem decoction. .

Cov lus tseem ceeb: Cistanche deserticola; Txheem decoction;Echinacoside; Piloside; Ntiv tes; Kev soj ntsuam zoo

"Cistanche deserticola yog ib tug qhuav, scaly, fleshy qia ntawm genus Cistanche nyob rau hauv Orobaceae los yog Cistanche tubulosa ma. Nws muaj cov teebmeem ntawm tonifying raum yang, pab cov ntshav essence, moistening cov hnyuv, thiab relieving cem quav. Nws yog dav siv nyob rau hauv impotence. , infertility, lub duav thiab lub hauv caug tenderness, nqaij thiab pob txha tsis muaj zog, thiab plab hnyuv dryness thiab cem quav."

cistanche hmoov cov txiaj ntsig kev noj qab haus huv-tonifying raum

Nyem qhov no mus saib Cistanche rau raum kab mob khoom

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Nws tsuas yog muaj phenylethanoid glycosides, benzyl cawv glycosides, iridoids thiab lawv cov glycosides, lignin thiab nws glycosides, thiab lwm yam. Ntawm lawv,phenylethanoid glycosidesraug suav hais tias yog cov khoom siv tseem ceeb hauv Cistanche deserticola ma.

Phenylethanol glycoside yog lub luag haujlwm tseem ceeb ntawm Cistanche deserticola

Tus txheej txheem decoction ntawm tsoos suav tshuaj slices (xws li, tus qauv decoction) yog ib qho decoction ntawm tsoos suav tshuaj slices npaj los ntawm cov txheej txheem txheem, coj los ntawm txoj kev xav ntawm cov tshuaj suav tshuaj thiab raws li kev kho mob daim ntawv thov, nrog rau kev siv niaj hnub extraction txoj kev. Vim lub fact tias tus txheej txheem decoction tsis muaj excipient cuam tshuam thiab tsis mus rau ib tug qhuav txheej txheem, nws yog raws li ib txwm decoction rau kev kho mob siv, thiab nws yog ib qho yooj yim mus ntsuam xyuas ntau yam zoo tagnrho concentrations los ntawm kev npaj ntawm slices los yog extracts. Yog li ntawd, tus qauv decoction ntawm tsoos suav tshuaj slices, raws li tus qauv zoo rau classic Ming Fang npaj thiab cov qauv siv rau kev ntsuas cov tshuaj suav tshuaj mis, tam sim no yog ib daim ntawv zoo ntawm kev ua kom muaj kev nyab xeeb, ua tau zoo, thiab ruaj khov ntawm cov tshuaj suav tshuaj. txhawm rau kom ua tiav qhov sib xws hauv kev kho mob. Tam sim no, tsis muaj cov ntaub ntawv qhia txog kev kawm txog tus qauv decoction ntawm Cistanche deserticola decoction pieces.Qhov kev sim no tau npaj cov txheej txheem decoction rau 14 pawg ntawm Cistanche deserticola, thiab tau tshawb fawb txog tus qauv decoction rau Cistanche deserticola, nrog rau kev pom los tsim cov qauv qauv ntawm cov cim zoo rau Cistanche deserticola, muab kev sim hauv paus rau kev txhim kho Cistanche deserticola formula granules. .

Cistanche hmoov

1.Apparatus thiab Materials

1.1 Cov cuab yeej thiab cov cuab yeej siv

Waterse2695 High-Performance Liquid Chromatography (Dej); ME204E 1/10000 tshuav nyiaj li cas (Mettler Toledo); XP26 lab scale (Mettler Toledo); WatersAcquire UPLC Ultra High-Performance Liquid Chromatography (WATSCH); KQ-500E Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

1.2 Reagents thiab cov ntaub ntawv

Echinacosidecov khoom siv (batch tooj: S-003-190403),anthocyaninCov khoom siv siv (batch tooj: G-066-181216),Acteosidecov khoom siv (batch tooj: M-011-181106), thiabisopilosidecov ntaub ntawv siv (batch naj npawb: Y{{0}}) tau tag nrho yuav los ntawm Chengdu Ruifensi Biotechnology Co., Ltd., nrog rau purity ntau dua 98.0 feem pua. Chromatographic ntshiab formic acid, Tianjin Kemio Chemical Reagent Co., Ltd; Chromatographic ntshiab methanol, Merck Co., Ltd; Kev sim dej yog lub chaw kuaj dej ultrapure dej, Merck Co., Ltd; Lwm cov reagents yog kev tshuaj ntsuam xyuas. Nyob rau hauv txoj kev tshawb no, tag nrho ntawm 14 pawg ntawm cov khoom npaj ntawm Cistanche deserticola tau sau los ntawm kev ua lag luam thiab txheeb xyuas los ntawm xibfwb Ma Zhiguo ntawm Jinan University raws li cov qhuav, scaly, fleshy stems ntawm genus Cistanche deserticola los yog Cistanche tubulosa, ib tsob nroj ntawm lub genus Cistanche hauv tsev neeg Orobaceae. Cov ntaub ntawv piv txwv (saib Table 1).

Echinoside, cov tshuaj tseem ceeb ntawm Cistanche deserticola

2 .Cov txheej txheem thiab cov txiaj ntsig

2.1 Kev npaj cov txheej txheem decoction thiab tshuaj ntsuam xyuas

2.1.1 Kev npaj ntawm Cistanche deserticola txheem decoction:

Raws li cov kev cai ntawm kev tswj cov tsoos suav tshuaj Decocting chav tsev nyob rau hauv cov tsev kho mob, Technical Requirements for Quality Control and Standard Formulation of granules of Traditional Chinese Medicine Formulas, thiab Standard Soup of Traditional Chinese Medicine, noj 100g ntawm cov npaj slices ntawm Cistanche deserticola , muab tso rau hauv lub lauj kaub hluav taws xob ceramic, ntxiv dej rau decoct ob zaug, ntxiv 9 zaug dej thawj zaug, soak rau 30min, kub thiab boil nrog Wuhuo (500W), kom lawv boil me ntsis nyob rau hauv me me tshav kub (200W), mus ntxiv. rau decoct rau 60min, thiab lim cov kua decocted los ntawm 350 mesh sieve thaum nws kub. Ntxiv 7 npaug ntawm cov dej rau lub sijhawm thib ob, ua kom sov tshaj qhov hluav taws kub, thiab coj mus rau boil. Hloov mus rau qis cua sov thiab ua kom nws npau taws me ntsis. Txuas ntxiv ua noj rau 40 feeb. Lim lub decoction nrog 350 mesh sieve thaum nws kub, thiab muab ob lub decoctions. Hloov cov decoction mus rau 2000mL puag ncig hauv qab lub raj mis, siv cov evaporator rotary kom txo qhov siab, thiab mloog zoo ntawm qhov kub thiab txias (kub: 60 degree; nqus tsev kawm ntawv: - 0.10MPa) mus rau qhov ntim tas li ntawm 500mL, thiab co zoo .

Table 1 Sample Information of Herba Cistanche

2.1.2 Kev npaj ntawm cov tshuaj ntsuam xyuas ntawm Cistanche deserticola decoction pieces:

Noj li 1g ntawm cov hmoov ntawm Cistanche deserticola (sieved los ntawm ib tug thib plaub sieve), raug luj nws, muab tso rau hauv ib tug 100mL xim av volumetric hwj, yog ntxiv 50mL ntawm 50 feem pua ​​methanol, kaw lub hau, co zoo. , ntsuas qhov hnyav, tsau rau 30min, kev kho ultrasonic rau 40min (lub zog: 250W, zaus: 35kHz), txias nws, hnyav qhov hnyav dua, ntxiv 50 feem pua ​​​​methanol los ua kom poob phaus, co zoo, cia nws sawv, noj lub supernatant, thiab dilute nws nrog 0.22 μ M microporous membrane pom, tau.

2.1.3 Kev npaj ntawm cov tshuaj ntsuam xyuas ntawm Cistanche deserticola txheem decoction:

Siv cov kua zaub cistanche, co zoo, ntsuas 5mL mus rau 50mL hauv lub ntim ntim, ntxiv cov ethanol anhydrous kom dilute mus rau qhov ntsuas, co zoo, thiab dilute nrog 0.22 μ M microporous membrane pom, tau.

2.1.4 Kev npaj cov kev daws teeb meem:

Siv tus nqi tsim nyog ntawm echinacea glycoside tswj cov qauv thiab cov qauv tswj hauv pas dej ua ke, ntsuas kom raug, muab tso rau hauv 10 mL xim av volumetric hwj, yaj lawv nrog 50 feem pua ​​methanol thiab dilute mus rau qhov ntsuas, co kom zoo, thiab npaj ib qho kev daws teeb meem nrog cov concentrations ntawm 0.0200 thiab 0.02100 mg · mL - 1 rau ob lub ntsiab lus saum toj no, raws li, kom tau txais cov tshuaj sib xyaw ua ke.

Acteoside, cov tshuaj tseem ceeb ntawm Cistanche deserticola

2.2 Cov ntsiab lus ntawm Echinoside thiab Piloside Cheebtsam

Txoj kev txiav txim siab cov ntsiab lus rau Cistanche deserticola hauv Volume I ntawm Suav Pharmacopoeia (2015 tsab) tau ua los ntawm Agilent ZORBAXB-C18 chromatographic kem (4.6 × 150mm,5 μ m) Siv methanol Raws li lub xov tooj ntawm tes A thiab 0.1 feem pua ​​formic acid tov raws li theem mobile B, gradient elution tau ua (0 mus rau 17 min, 26.5 feem pua ​​A; 17 mus rau 20 min, 26.5 feem pua ​​→ 29.5 feem pua ​​A; 20 mus rau 27 min, 29.5 feem pua ​​A); Tus nqi ntws: 1.0mL · min-1; Kem kub: 30 degree; Ntsuas wavelength: 330nm; Tus naj npawb ntawm cov phaj theoretical xam raws li lub ncov ntawm echinacea yuav tsum tsis txhob tsawg tshaj li 3000. Cov ntsiab lus ntawm echinacea glycoside thiab poolside nyob rau hauv cov khoom npaj ntawm Cistanche deserticola thiab lawv cov qauv decoction tau txiav txim siab, thiab cov txiaj ntsig tau qhia hauv Table 2.

2.3 Kev txiav txim siab ntawm kev soj ntsuam cov yam ntxwv ntawm cov qauv decoction ntawm Cistanche deserticola decoction pieces

Table 2 Cov Kev Ntsuas Zoo ntawm Herba Cistanche thiab Nws Cov Txheej Txheem Decoction (n=2)

2.3.1 Paste yield:

Co cov txheej txheem Cistanche deserticola kua zaub npaj nyob rau hauv 2.1.1 zoo, yog nqus 100mL, muab tso rau hauv lub qhov hnyav evaporation phaj (qhov hnyav: m1), evaporate hauv dej da dej, qhuav hauv qhov cub ntawm 105 degree rau 3h, noj. Nws tawm, muab tso rau hauv lub tshuab ziab khaub ncaws kom txias thiab qhuav rau 30 feeb, ntsuas nws, sau nws qhov hnyav li m2, thiab qhov hnyav ntawm cov khoom siv tshuaj raws li M. Raws li cov qauv, "Cream yield=(m2 m1 )" × 500 / (100 × M) × xam tus nqi muab tshuaj txhuam los ntawm "100 feem pua", thiab muab tus nqi nruab nrab tom qab 2 daim ntawv luam ntawm txhua tus qauv. Cov txiaj ntsig (saib Table 2).

2.3.2 Hloov pauv tus nqi:

Raws li cov kev ntsuas saum toj no ntawm cov ntsiab lus ntawm echinacea glycosides thiab pilosides, kev hloov pauv ntawm echinacea glycosides thiab pilosides raug xam raws li cov qauv hloov pauv (hloov tus nqi= zoo ntawm qhov ntsuas cov khoom hauv Cistanche deserticola tus qauv decoction / zoo. ntawm qhov taw qhia cov khoom hauv decoction pieces × 100 feem pua ​​​​), 2 parallel luam rau txhua tus qauv. Cov txiaj ntsig (saib Table 2).

2.3.3 pH tus nqi:

Co lub Cistanche deserticola tus qauv decoction npaj nyob rau hauv 2.1.1 tusyees, coj tus nqi tsim nyog, ntsuas pH tus nqi nrog pH meter, thiab coj tus nqi nruab nrab. Txhua tus qauv muab faib ua ob ntu nyob rau hauv parallel. Cov txiaj ntsig (saib Table 2).

2.4 Tsim kom muaj UPLC ntiv tes ntawm Cistanche deserticola tus qauv decoction

2.4.1 Chromatographic tej yam kev mob:

Siv ib kem WatersHSST3 (2.1 × 100mm, 1.8 μ m); Siv methanol li mobile theem A thiab 0.1 feem pua ​​formic acid tov raws li mobile theem B, gradient elution tau ua (0 rau 4 min, 34 feem pua ​​A; 4 mus rau 10 min, 34 feem pua ​​→ 40 feem pua ​​A; 10 txog 15 min, 40 feem pua ​​→ 46 feem pua ​​A; 15 txog 17 min, 46 feem pua ​​→ 34 feem pua ​​A; 17 txog 20 min, 34 feem pua ​​A); Tus nqi ntws: 0.3mL · min-1; Kem kub: 30 degree; Ntsuas wavelength: 330nm; Kev siv tshuaj txhaj tshuaj ntim 1 μ 50. Kev txhaj tshuaj ntawm cov tshuaj 0.4 μ L.

2.4.2 Kev npaj cov kev daws teeb meem:

Noj cov tshuaj uas tsim nyog ntawm Echinacetin, Angioside A, Piloside, thiab Isopiloside, ua kom hnyav rau lawv, muab tso rau hauv 10mL xim av volumetric hwj, yaj lawv nrog 50 feem pua ​​methanol, dilute. mus rau qhov ntsuas, co zoo, thiab npaj cov kev daws teeb meem nrog cov concentrations ntawm plaub yam saum toj no ntawm 0.196, 0.234, 0.228, thiab 0.213 mg · mL - 1, raws li, kom tau txais kev sib xyaw tswj kev daws teeb meem.

2.4.3 Qhov kev npaj ntawm cov tshuaj ntsuam xyuas yog tib yam li hauv Yam 2.1.3.

2.4.4 Precision xeem:

Noj cov tshuaj ntsuam xyuas ntawm tib batch ntawm Cistanche deserticola txheem decoction (GH{{0}}) thiab txhaj nws rau lub sij hawm tsis tu ncua raws li cov xwm txheej chromatographic hauv 2.4.1. Siv lub pas dej ua ke (nruab nrab 4) raws li kev siv lub ncov, suav tias RSD ntawm cov txheeb ze tuav lub sijhawm ntawm txhua qhov siab tshaj yog tsawg dua 0.2 feem pua ​​, thiab RSD ntawm thaj chaw txheeb ze yog tsawg dua 5.0 feem pua ​​, qhia tias qhov ntsuas tau zoo precision.

Kev Tshawb Fawb Txog Cistanche deserticola

2.4.5 Stability Test:

Siv cov tshuaj ntsuam xyuas tib yam ntawm Cistanche deserticola standard decoction (GH{{0}}}) thiab txhaj tshuaj ntawm 0, 2, 4, 8, 12, 16, 20, 24, thiab 28 teev tom qab kev npaj raws li cov xwm txheej chromatographic hauv 2.4.1. Siv lub pas dej ua ke (nruab nrab 4) raws li kev siv lub ncov, suav tias RSD ntawm cov txheeb ze tuav lub sij hawm ntawm txhua qhov siab tshaj yog tsawg dua 0.3 feem pua, thiab RSD ntawm thaj chaw txheeb ze yog tsawg dua 4.8 feem pua, qhia tias qhov kev sim tshuaj muaj zoo stability nyob rau hauv 28 teev.

2.4.6 Repeatability test:

Siv tib lub pob ntawm Cistanche deserticola standard decoction (GH{{{0}}}), npaj 6 qhov chaw ntawm cov tshuaj nyob rau hauv parallel raws li txoj kev nyob rau hauv yam khoom 2.1.3, soj ntsuam raws li cov chromatographic tej yam kev mob nyob rau hauv yam khoom 2.4. 1, sau cov chromatogram, thiab xam tias RSD ntawm cov txheeb ze tuav lub sij hawm ntawm txhua qhov siab tshaj plaws yog tsawg dua 0.2 feem pua, thiab RSD ntawm thaj chaw txheeb ze tsawg dua 3.8 feem pua. Cov txiaj ntsig tau pom tias qhov rov ua dua ntawm txoj kev yog qhov zoo.

2.4.7 Tsim cov ntiv tes:

Npaj cov tshuaj ntsuam xyuas ntawm 14 batch ntawm Cistanche deserticola tus qauv decoction raws li Yam Khoom 2.1.3, thiab txhaj cov qauv rau kev txiav txim siab raws li cov xwm txheej chromatographic hauv Yam 2.4.1 kom tau txais UPLC ntiv tes ntawm 14 batch ntawm Cistanche deserticola standard decoction. UPLC ntiv tes chromatograms ntawm 14 pawg ntawm cov tshuaj ntsuam xyuas tau raug xa mus rau "Suav Tshuaj Chromatographic Fingerprint Similarity Evaluation System" (Version 2{{10}}}12) software, siv SI raws li kev siv chromatogram, ncov sib xws yog ua nrog lub sij hawm dav ntawm 0.1, thiab cov txheej txheem nruab nrab tau siv los tsim cov ntawv siv ntiv tes R ntawm Cistanche deserticola txheem kua zaub (saib daim duab 1). Tib lub sijhawm, qhov zoo sib xws tau suav, thiab cov txiaj ntsig tau txais (saib Table 3) tau txais. Los ntawm daim ntawv qhia sib piv, nws tuaj yeem pom tias muaj 6 qhov siab tshaj plaws nyob rau hauv tus qauv decoction ntawm Cistanche deserticola. Raws li thaj chaw ncov thiab lub sijhawm khaws cia ntawm rau qhov siab tshaj plaws, lub sijhawm khaws cov txheeb ze (nruab naj npawb (nruab nrab txheeb ze lub sijhawm khaws cia) tau suav tias yog ncov 1 (0.434), ncov 2 (0) .643), ncov 3 ({{40}}}.919), ncov 4 (1.000), ncov 5 (1.635), thiab ncov 6 (1.858), ntsig txog; txheeb ze ncov thaj chaw, [peak tooj (nruab nrab thaj tsam ncov)] tau ncov 1 (6.340), ncov 2 (0.890), ncov 3 (0.295), ncov 4 ( 1.000), ncov 5 (0.674), thiab ncov 6 (0.0190), feem. thiab ncov 5 tau txheeb xyuas tias yog isopiloside; Qhov kev soj ntsuam zoo sib xws tau pom tias qhov zoo sib xws ntawm 14 batch ntawm tus qauv decoction thiab daim ntawv qhia tswj yog nyob nruab nrab ntawm 0.789 thiab 0.999 (saib daim duab 2).

Table 3    14 Zoo sib xws ntawm UPLC ntiv tes ntawm batch Cistanche deserticola tus qauv decoction

Daim duab 1 UPLC chromatograms ntawm mixed reference tov (A), cistanche deserticola standard decoction (B), thiab cistanche tubulosa standard decoction (C)

1. Echinoside; 3. Anthocyanin A; 4. Physalis glycosides; 5. Isopiloside

Daim duab 2    14 UPLC ntiv tes ntawm batch Cistanche deserticola txheem decoction

3 .Kev sib tham

3.1 Kev npaj cov txheej txheem decoction

Raws li tus qauv zoo, tus qauv decoction ntawm tsoos suav tshuaj slices standardizes txawv cov ntaub ntawv kho mob, uas yog tam sim no ib tug ntawm cov kev tshawb fawb hotspots nyob rau hauv lub teb ntawm tsoos suav tshuaj. Kev npaj ntawm tus txheej txheem decoction yuav tsum tau hais tias lub hauv paus ntawm cov decoction pieces yog qhabnias thiab sawv cev. Yog li, Suav Pharmacopoeia suav nrog 9 pawg ntawm Cistanche deserticola thiab 5 pawg ntawm Cistanche deserticola npaj. Ua ntej kev npaj, cov ntawv npaj tau raug sim thiab txhua yam ua tau raws li qhov yuav tsum tau ua ntawm 2015 tsab ntawm Suav Pharmacopoeia.

3.2 Kev soj ntsuam ntawm qhov kev txhaj tshuaj ntawm cov tshuaj ntsuam xyuas

Hauv kev txiav txim siab cov ntsiab lus ntawm qhov ntsuas qhov ntsuas hauv cov qauv decoction, tus qauv txhaj tshuaj ntawm cov ntawv xeem yog 10 μ Ntawm L, tsis muaj qhov ncov ntawm pas dej hauv tus qauv decoction chromatogram ntawm Cistanche deserticola, tshwj tsis yog rau echinacea glycosides. Txawm hais tias ob qho tib si echinacea glycoside thiab poolside tshwm sim nyob rau hauv tus qauv decoction chromatogram ntawm Cistanche deserticola, lub ncov cheeb tsam ntawm ob qho tib si echinacea glycoside thiab pas dej ua ke yog me me, thiab nws tau pom tias lub siab ncov tau tshwm sim ntawm qhov hnyav ncov txoj hauj lwm hauv chromatogram ntawm cov ntawv xeem. Yog vim li cas rau kev tsom xam yog tias qhov ntim tas li ntawm cov qauv kuaj yog anhydrous ethanol tsis muaj dej, thiab nws tsis sib haum nrog kev npaj hnyav ntawm cov qauv tswj, 50 feem pua ​​​​methanol, ua rau muaj qhov hnyav hnyav, ua rau tsis muaj kev tuav pov hwm ntawm echinacea. thiab sab laug. Qee cov qauv muaj qhov siab tshaj ntawm echinacea thiab muab vim lawv cov ntsiab lus siab thiab khaws cia ib nrab. Raws li kho qhov kev txhaj tshuaj ntawm cov ntawv xeem mus rau 5 thiab 3 μ L Tshawb xyuas qhov kev tshem tawm cov nyhuv hnyav, thiab qhov tshwm sim qhia tau hais tias tus qauv txhaj tshuaj ntawm cov ntawv xeem yog 3 μ ntawm L, cov nyhuv hnyav tuaj yeem raug tshem tawm, thiab Lub ncov ntawm echinacea tuaj yeem yog qhov qub, yog li cov qauv txhaj tshuaj ntawm cov ntawv xeem tau teem rau 3 μ L. Ib yam li ntawd, cov nyhuv hnyav tsim tawm thaum lub sij hawm tus ntiv tes txiav txim siab ntawm tus qauv decoction raug tshem tawm los ntawm kev tshawb xyuas qhov txhaj tshuaj ntawm cov tshuaj ntsuam xyuas, thiab qhov kev txhaj tshuaj zoo tshaj plaws tau txiav txim siab yog 0.4 μL.

3.3 Yog vim li cas rau qhov tsis zoo sib xws ntawm cov ntiv tes ntawm Cistanche deserticola tus qauv decoction

Qhov zoo sib xws ntawm cov ntiv tes ntawm 14 batch ntawm Cistanche deserticola thiab Cistanche tubulosa tus qauv decoction ranged ntawm 0.789 mus rau 0.999. Qhov qis tshaj qhov zoo sib xws yog RCR-9 nrog cov kua zaub txheem ntawm 0.789. Qhov zoo sib xws ntawm lwm cov batch yog saum toj no 0.98. Piv nrog rau lwm cov qauv, tag nrho qhov zoo sib xws ntawm RCR-9 txheem kua zaub tsis siab. Los ntawm tus ntiv tes chromatogram ntawm RCR-9 qauv kua zaub, nws tuaj yeem pom tau tias nws muaj ob qhov pom tseeb tshaj plaws piv rau lwm cov qauv ntawm lub sijhawm tuav ntawm 12.278 thiab 15.466. Vim yog cov khoom siv tshuaj lom neeg hauv cov tshuaj suav tshuaj, qhov sib txawv ntawm qhov chaw loj hlob, lub sijhawm sau qoob loo, cov txheej txheem ua, thiab lwm yam ua rau cov khoom sib txawv thiab cov ntsiab lus ntawm cov khoom npaj. Qhov tsis zoo sib xws ntawm RCR-9 cov kua zaub txheem yuav yog vim muaj ntau yam uas ua rau cov khoom tsub zuj zuj ntawm cov batch ntawm cov khoom npaj yuav txawv ntawm lwm cov khoom.

3.4 Kev tswj zoo ntawm Cistanche deserticola tus qauv decoction

Qhov kev sim no tau tshuaj xyuas tus nqi rho tawm, tus nqi pH, thiab kev hloov pauv ntawm echinacoside thiab pas dej ua ke ntawm 14 pawg ntawm cistanche deserticola standard decoction. Tus nqi rho tawm ntawm 14 batch ntawm Cistanche deserticola tus qauv decoction ranged ntawm 25.1 feem pua ​​​​rau 56.6 feem pua. Vim tias cov ntsiab lus qab zib tsawg ntawm cov khoom npaj ntawm Cistanche deserticola piv rau cov khoom npaj ntawm Cistanche deserticola, tus nqi rho tawm ntawm tus qauv decoction ntawm Cistanche deserticola deserticola yog qis dua li cov qauv decoction ntawm Cistanche deserticola. Tus nqi pH ntawm 14 pawg ntawm Cistanche deserticola tus qauv decoction ranged ntawm 4.30 txog 5.15, qhia tias pH tus nqi ntawm Cistanche deserticola tus qauv decoction yog qhov ruaj khov. Qhov nruab nrab hloov pauv ntawm Echinacetin hauv 14 pawg ntawm Cistanche deserticola thiab Cistanche deserticola tus qauv decoction yog 22.99 feem pua, thiab qhov nruab nrab hloov pauv ntawm pas dej ua ke yog 17.09 feem pua. Ntawm lawv, qhov kev hloov pauv ntawm cov khoom lag luam hauv cov qauv decoction npaj los ntawm Cistanche deserticola decoction pieces yog qhov ruaj khov, thaum hloov pauv tus nqi ntawm cov khoom lag luam hauv cov txheej txheem decoction npaj los ntawm Cistanche deserticola decoction pieces yog qhov loj ntawm cov khoom siv.

4.Xaus

Hauv txoj kev tshawb no, UPLC txoj kev tau siv los tsim cov ntiv tes ntawm Cistanche deserticola tus qauv decoction, uas tuaj yeem ua rau muaj kev cuam tshuam thiab ntsuas qhov zoo ntawm Cistanche deserticola tus qauv decoction. Cov txiaj ntsig ntawm kev rho tawm tus nqi, qhov ntsuas qhov ntsuas qhov hloov pauv, thiab ntsuas ntiv tes ntawm tus qauv cistanche decoction tau pom tias tag nrho homogeneity ntawm 14 pawg ntawm cistanche standard decoction npaj tau zoo, txoj kev npaj tau zoo raws li kev kho mob, thiab qhov zoo yog tswj tau. Qhov no yuav pab tsim cov qauv sib txawv ntawm cov tshuaj cistanche, xyuas kom meej qhov sib xws ntawm cov tshuaj siv tshuaj kho mob, thiab daws qhov zoo sib txawv los ntawm cov txheej txheem ntau lawm thiab kev npaj.

Superman tshuaj ntsuab cistanche

siv

〔1〕 Kho los ntawm National Pharmocopoeia Commission. Suav Pharmacopoeia (Part I) [S]. Beijing: Tuam Tshoj Pharmaceutical Science thiab Technology Xovxwm, 2015135

〔2〕Li Chenghua, Liu Jinghui, Xu Zhenxia, ​​et al. Kev txiav txim siab ib txhij ntawm plaub phenylethanol glycosides hauv Cistanche tubulosa los ntawm RP-HPLC. Phau ntawv Journal of Pharmaceutical Analysis, 2010, 30 (06): 1003-1006

〔3〕Ding Yan, Zhang Kaimei, Cang Xiaoxin, et al. Kev tshawb fawb txog cov khoom siv tshuaj lom neeg thiab cov dej num lom neeg ntawm Cistanche L. Journal of Dalian University of Technology, 2016, 35 (06): 395-402

〔4〕Gao Yan, Guo Linong, Ma Shuangcheng, et al. Kev sib piv ntawm peb hom cistanche deserticola raws li UPLC tus yam ntxwv daim ntawv qhia thiab cov ntsiab lus tseem ceeb ［ J］. Phau ntawv Journal of Traditional Chinese Medicine, 2019, 44 (17): 3749-3757

〔5〕Chen Shilin, Liu An, Li Qi, et al. Kev tshawb fawb lub tswv yim rau tus qauv decoction ntawm tsoos suav tshuaj slices ［J］. Suav Journal of Traditional Chinese Medicine, 2016, 41 (08): 1367-1375