Kawm Txog Kev Ua Haujlwm Antioxidant Ntawm Phenylethanoid Glycosides Los Ntawm Cistanche Deserticola
Mar 10, 2022
Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com
LI Li et al (Central Laboratory of Changchun Normal University, Changchun, Jilin 130032)
Abstract[Lub Hom Phiaj] Cov kev tshawb fawb tsom los kawm txog kev ua haujlwm ntawm cov tshuaj tiv thaiv antioxidant ntawm phenylethanoid glycosides los ntawmCistanchedeserticola. [Txoj Kev] Ob lub qauv qauv, cov dawb radical 2, 2-diphenyl-l -picrylhydrazyl (DPPH ') assay thiab daim duab chemiluminescence (PCL) assay, tau siv los ntsuas cov tshuaj antioxidant ntawm C. deserticola extract, acteoside, isoacteoside, thiab echinacoside. [Qhov tshwm sim] Cov txiaj ntsig tau pom tias tag nrho cov ntsiab lus phenolic (TPCs) hauv echinacoside, acteoside, isoacteoside, thiab 80 feem pua ethanol extract ntawm C. deserticola yog 753.95, 659.94, 356.14, thiab 14.73 mg gallic acid / g qauv. C. deserticola extract tau pom muaj zog antiox记ant kev ua haujlwm hauv PCL kev sim, ua raws li echinacoside, acteoside, thiab isoacteoside. Txawm li cas los xij, echinacoside nthuav tawm muaj zog antioxidant kev ua haujlwm hauv DPPH experiment, ua raws li acteoside, isoacteoside, thiab C. deserticola extract.
[Xaiv]Qhov kev tshawb fawb no tuaj yeem muab cov ntaub ntawv pov thawj rau pharma cology thiab kev tshawb fawb txog kev ua haujlwm lom neeg ntawm phenylethanoid glycosides los ntawm C. deserticola.
Cov lus tseem ceeb Cistanche deserticola ; Acteoside; Isoacteoside; Echinacoside; Antioxidant kev ua haujlwm; DPPH; PCL
Cistanche (Cistanche deserticolaYC Ma.) is a plant of theCistanchegenus (Oro-banehaeeeae) ntawm lubCistanchegenus (Cistanche). Lub qia qhuav ntawm Cistanche nrog scaly nplooj. Ntau cov kev tshawb fawb tau pom tias cov khoom xyaw tseem ceeb ntawm Cistanche cov nroj tsuag yog phenethyl cawv glycoside tebchaw. . Phenylethanol glycoside compound belongs rau ib hom polyphenol compound, uas yog dav faib rau ntau hom nroj tsuag. Cov kev tshawb fawb tshuaj pharmacological tau pom tias cov tshuaj no muaj ntau yam tshuaj thiab tshuaj lom neeg, xws li tshuaj tiv thaiv hepatotoxin, tshuaj tiv thaiv kab mob, tshuaj tua kab mob, thiab tshuaj tua kab mob antioxidant. Ntawm lawv, qhov tseem ceeb tshaj plaws yog tias phenoxyethanol glycoside compound muaj cov tshuaj antioxidant zoo heev. Tus sau tsuas yog ua ib qho kev soj ntsuam ntawm cov tshuaj antioxidant ntawm phenethyl cawv glycoside tebchaw hauvCistanche, siv ob txoj kev ntsuas tshuaj tiv thaiv oxidant hauv vitro, uas yog Photoehemilumineseence (PCL) thiab 2, 2-diphenyl-1-pieryl-hydroxyl (DPPH') Ob lub tshuab sib txawv tau siv los ntsuas cov kev ua haujlwm antioxidant ntawm 80. feem pua ethanol extract ntawmCistanche, tshuaj ergosteroside, isoergoside cov, thiabechinacoside cov tshuaj.
1 Cov khoom siv thiab cov txheej txheem
1.1 Samples thiab reagents
Cistanche (Cistanche deserticola)Cov khoom siv tshuaj tau txais los ntawm Beijing Tongrentang Pharmacy.Ergosteroside, isoergoside,echinacoside cov tshuaj, Folin. Ciocalteu reagent, gallic acid, 2, 2-diphenyl-1-picrylhydrazyl (DPPH.), thiab lwm yam. tau yuav los ntawm SigmaChemicalCo. Tuam txhab (St. Louis, Mo). Cov kuab tshuaj siv rau PCL tsom xam yog muab los ntawm Analytik Jena AG (Berlin, Lub teb chaws Yelemees). Lwm cov reagents yog tag nrho cov tshuaj analytical reagents (tsim los ntawm Beijing Chemical Plant). Cov dej yog dej ntshiab (18.2 nPa).
1.2 Qauv rho tawm
Qhov hnyav yog 2.0005g ntawmCistancheqauv hmoov, siv 50rnl ntawm 80 feem pua ethanol rau ultrasonic extraction ntawm chav tsev kub rau 0.5h, extract ob zaug, lim cov qauv tov, teb evaporate rau dryness (<40oc), use="" 100ml="" of="" distilled="" water="" dissolve,="" degrease="" twice="" with="" 100ml="" n-hexane,="" discard="" the="" n-hexane="" solution,="" extract="" the="" water="" part="" with="" 100="" ml="" of="" water-saturated="" n-butanol="" 3="" times,="" combine="" the="" n-butanol="" extracts,="" rotary="" evaporate="" to=""><40oc), and="" use="" 4ml="" for="" the="" residue="" the="" volume="" of="" absolute="" ethanol="" is="" fixed="" to="" obtain="" a="" sample="" of="">Cistanche deserticola.
1.3 Kev txiav txim siab ntawm tag nrho cov ntsiab lus polyphenol ntawm cov qauv
Tag nrho cov ntsiab lus phenol ntawm tus qauv yog txiav txim siab siv F{{0}} lin-Ciocaheu txoj kev, thiab qhov tshwm sim yog qhia raws li milligrams ntawm gallic acid ib gram ntawm extract. Noj ib qho kev daws teeb meem nrog ib qho concentration ntawm 0.2rnI (kom lub absorbance poob ntawm tus qauv nkhaus), ntxiv 1ml ntawm Folin-Ciocaheu reagent nrog ib tug concentration ntawm 0.5mol / L, thiab Tom qab ntawd ntxiv 0.8ml ntawm sodium carbonate tov nrog ib tug concentration ntawm 7.5 feem pua kom sib tov zoo, Muab tso rau hauv chav tsev kub rau 0.5h, thiab ces ntsuas qhov absorbance ntawm 765nm (ultraviolet-pom spectrophotometer: DU800, Beckman Couher, Inc. USA). Siv cov tshuaj gallic acid (qhov loj ntawm qhov ntau yog 0.02-0.20me,/m1) los ua tus qauv nkhaus, thiab tag nrho cov kev ntsuam xyuas tau rov ua dua ob zaug.
1.4 Antioxidant kev ua haujlwm hauv vitro tsom xam
1.4.1 PCL txoj kev txiav txim.
Qhov kev sim siv Photochem @ (Berlin, Ger-ntau) ultra-ceev antioxidant thiab dawb radical tsis siv neeg analyzer. Lub hauv paus ntsiab lus yog los tsim cov dawb radicals los ntawm cov txheej txheem photochemical thiab siv cov txheej txheem chemiluminescence kom pom cov dawb radicals. Hauv kev sim, cov duab hluav taws xob nrog cov nyhuv photochemical excitation tau siv los ua kom cov tshuaj tiv thaiv molecules, yog li nyob rau hauv qhov kev txiav txim ntawm lub teeb ultraviolet, cov tshuaj tiv thaiv oxidation tshwm sim 1000 zaug sai dua li cov xwm txheej ib txwm muaj, thiab cov dawb radicals tau tsim sai. " Xaiv txoj kev PCL-ACW-Kit Ntsuas cov tshuaj tiv thaiv antioxidant ntawm cov dej-soluble Cheebtsam ntawm psyllium sub-samples. Pipette 1.0ml cov tshuaj tiv thaiv tsis zoo (nrog rau cov concentration ntawm 0.1moL/L SodiumCarbonate, pH nqi ntawm 10.5 thiab concentration ntawm 0.1mmol/LNA, ib EDTA, 1.5ml ACW- diluent (dej ), concentration 1mmol / LLumino1) ua raws li photosensitizer kom zoo siab rau cov tshuaj tiv thaiv molecules, kom dawb radicals yog sai generated, thiab nyob rau tib lub sij hawm raws li ib tug photochemiluminescent tshuaj. Los ntawm kev ntsuas qhov sib txawv (0.5, 1.0, 2.0 thiab 3.0 nmol / L) ntawm L. Tus qauv nkhaus sib npaug rau ascorbic acid yog Y=24.640Ox ntxiv rau 2.9311, R²=0.9969. Nws yog ib qho tsim nyog yuav tsum tau dilute cov qauv los yog cov qauv kev daws teeb meem hauv kev sim kom lub sij hawm lag luam poob rau hauv qhov sib txawv ntawm cov qauv nkhaus. Photochem tsim cov qauv nkhaus thiab kuaj cov tshuaj tiv thaiv antioxidant ntawm cov qauv txiav, thiab cov tshuaj tiv thaiv antioxidant ntawm cov qauv yog sib npaug rau L. Muab xam los ntawm tus naj npawb ntawm nanomoles ntawm ascorbic acid.
1.4.2 DPPH txoj kev txiav txim siab.
Diphenylpicrylhydrazino radical [DPPH ·] yog ib qho ruaj khov nitrogen-centered radical nrog siab tshaj plaws nqus ntawm lub wavelength ntawm 515nm. [DPPH ·] cov tshuaj methanol yog ntshav, thiab nws cov concentration muaj kev sib raug zoo nrog absorbance. ] Tus naj npawb ntawm cov dawb radicals txo qis, thiab cov xim ntawm cov tshuaj yuav sib zog, uas yog manifested los ntawm qhov tsis tu ncua ntawm cov absorbance ntawm lub wavelength ntawm 515 nm mus txog rau thaum nws mus txog stability." Hauv kev sim, ethanol tau siv los hloov methanol. Ua kom hnyav [DPPH ·] thiab teeb tsa cov concentration rau 0. [DPPH ·] ethanol tov ntawm 0.040 76mg/ml, tus qauv nkhaus ntawm [DPPH ·] tau: Y: 18.032x-0.0016 , R=0.9993. Txoj kev ntsuas qhov seem seem ntawm cov dawb radicals hais txog cov ntaub ntawv [16]. Siv ethanol ua cov kuab tshuaj, npaj cov kev sib txawv ntawm cov qauv thiab cov qauv kev daws teeb meem, noj 0.1ml qauv lossis cov qauv kev daws teeb meem raws li, thiab ntxiv 3.9ml [DPPH·] cov qauv kev daws teeb meem nrog rau qhov loj ntawm 2.5mg / L (tam sim no siv tam sim no), hloov cov qauv kev daws teeb meem nrog ethanol ua ib qho khoob. Co cov tshuaj sib tov, siv lub cuvette los ntsuas nws qhov nqus ntawm lub sijhawm sib txawv ntawm lub wavelength ntawm 515nm, thiab hloov nws mus rau qhov loj concentration ntawm [DPPH·] raws li tus qauv nkhaus los xam cov seem ntawm [DPPH·]. Raws li tus nqi seem ntawm [DPPH ·] thiab qhov sib npaug ntawm cov qauv ntxiv, qhov nkhaus rau tshem tawm [DPPH -] dawb radicals los ntawm cov qauv tuaj yeem kos. Raws li kev sib raug zoo nkhaus ntawm qhov loj concentration ntawm [DPPH ·] thiab lub absorbance, lub absorbance ntsuas tom qab ntxiv cov dawb radical scavenger yuav hloov mus rau hauv lub loj concentration ntawm [DPPH ·] los laij lub [DPPH ·] tom qab lub ntxiv cov dawb radical scavenger. Cov mis laij lej rau tus nqi seem yog raws li hauv qab no: [DPPH ·]REM=[DPPH·]/[DPPH·]r=0 × 100 feem pua qhov twg [DPPH·] yog qhov loj ntawm DPPH · nyob rau hauv ib lub sij hawm nyob rau hauv lub dawb radical scavenging txheej txheem, [DPPH·]:. Yog thawj pawg loj ntawm DPPH ·. Raws li tus naj npawb ntawm cov antioxidants ntxiv thiab cov seem seem ntawm [DPPH ·], kev sib raug zoo nkhaus ntawm ob yog ua, thiab qhov sib npaug regression tuaj yeem tau los ntawm kev txheeb xyuas kab rov tav. Raws li qhov sib npaug regression, cov tshuaj tua kab mob antioxidant thaum qhov seem ntawm [DPPH ·] yog 50 feem pua yuav suav tau. Ib nrab inhibition. Raws li [DPPH·] dawb radical scavenging nkhaus ntawm ntau qhov sib txawv ntawm cov qauv lossis cov qauv, suav cov nyiaj EC ntawm cov qauv thaum qhov tseem ceeb ntawm [DPPH·] raug txo mus rau 50 feem pua (steady-state). Qhov me me ntawm EC, cov dawb radical scavenging muaj peev xwm Lub zog "Kuaj 3 zaug nyob rau hauv parallel.
cistanche
2 Cov txiaj ntsig thiab kev txheeb xyuas
2.1 Kev txiav txim siab ntawm tag nrho cov ntsiab lus polyphenol ntawm cov qauv Hauv kev txiav txim siab ntawm tag nrho cov ntsiab lus polyphenol, tag nrho cov ntsiab lus polyphenol ntawmechinacoside cov tshuajyog qhov siab tshaj plaws, uas yog 753.95 mg gallic acid / g. Ua raws li ergosteroid glycosides, isoergosteroid glycosides, thiab crude extracts ntawmCistanche, uas yog 468.3, 356.14, thiab 14.73 mg gallic acid / g, feem.
2.2 Kev txiav txim siab ntawm cov qauv tshuaj tiv thaiv antioxidant
2.2.1 PCL- ACW txoj kev txiav txim siab. Hauv PCL-ACW tshuaj tua kab mob antioxidant, lub peev xwm antioxidant ntawm 1txgCistanchecov khoom siv tshuaj yog sib npaug rau lub peev xwm antioxidant ntawm 15.68nmol L-ascorbic acid; 1g ibechinacoside cov tshuajyog sib npaug rau lub peev xwm antioxidant ntawm 14.03nmol ascorbic acid Kev ua: 1txgtshuaj ergosterosideyog sib npaug rau 11.44nmolL lub peev xwm antioxidant ntawm ascorbic acid; 1Ixg isomergosteroside yog sib npaug rau cov haujlwm antioxidant ntawm 8.24nmol ascorbic acid.
2.2.2 DPPH txoj kev ntsuas ntsuas. Nws tuaj yeem pom los ntawm Table 1 tias nws raug tshem tawm. Qhov kev txiav txim ntawm lub peev xwm ntawm cov dawb radicals yogtshuaj ergosteroside, echinacoside cov tshuaj, isoergoside thiab crude extract ntawmCistanche, ntawm cov uas ergo sab thiabechinacoside cov tshuajmuaj qhov tseem ceeb tib lub peev xwm antioxidant hauv DPPH xeem. Nws tuaj yeem pom los ntawm qhov no. Vim tias qhov sib txawv hauv vitro antioxidant kuaj tshuab yog siv los ntsuas cov qauv, cov txheej txheem ntawm cov teebmeem antioxidant txawv, thiab cov txiaj ntsig yuav tsis sib haum. Qhov no kuj tau raug txheeb xyuas hauv qhov kev xeem. Yog li ntawd, nws yog ib qho tsim nyog los xaiv lwm cov tshuaj tiv thaiv antioxidant rau kev tshawb xyuas ntxiv.
echinacosie kev
cov ntaub ntawv
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