Kev Tshawb Fawb Txog Cov nyhuv sedative ntawm Cistanche Deserticola

Yog xav paub ntxiv:Ali.ma@wecistanche.com

Ming-Chin Lu

Department of Suav Diagnosis, Tuam Tshoj Medical College, 91 Hsieh Shih Road, Taichung, Taiwan, ROC

Abstract

Hauv qhov kev tshawb fawb no, peb tau tshawb nrhiav qhovtshuaj sedativenyhuvntawmCistanchedeserticolaMa. (CD) ntawm hexobarbital-induced pw tsaug zog nyob rau hauv nas thiab spontaneous lub cev muaj zog ua si los ntawm kev siv ib tug automated kev ntsuas ntsuas nyob rau hauv nas. Nws tau pom tias crudeextract ntawm CD(Cistanchedeserticola)tuaj yeem ua kom ntev lub sijhawm pw tsaug zog hexobarbital thiab txo qis kev ua haujlwm ntawm lub cev muaj zog, suav nrog kev ua haujlwm kab rov tav, lub sijhawm thauj mus los, thiab tag nrho qhov nrug. Tom qab ntawd cov dej feem ntawm CD(Cistanchedeserticola)Extract tuaj yeem ncua lub sijhawm pw tsaug zog ntawm hexobarbital thiab txo cov kev ua haujlwm ntawm lub cev ntau dua li ntawm lwm feem ntawm CD.(Cistanchedeserticola)extract hauv nas. Cov txiaj ntsig no qhia tias CD ethanol extract thiab nws cov dej feem muaj covtshuaj sedativenyhuv. © 1998 Elsevier Science Ireland Ltd.

Ntsiab lus: Cistanche deserticola; Kev ntxhov siab; Automated kev ntsuas ntsuas; Kev ua haujlwm ntawm lub cev muaj zog

Cistanche deserticolamuaj ibsedative nyhuv

Nyem rau cistanche deserticola ma cov khoom

1. Taw qhia

CistanchedeserticolaMa. (Orobanchaceae)(CD) yog suav tshuaj tshuaj, dav siv hauv kev kho mob insomnia, mob, qaug zog, andamnesia (Lee, 1986). Hauv kev tshawb fawb pharmacological niaj hnub, ethanol extract ntawm CD(Cistanchedeserticola)tau pom tias muaj cov teebmeem kev laus (Lee et al., 1990) thiab txhim kho lub cev tsis muaj zog (Chin thiab Su, 1993).Dej extract ntawm CD extract nce DNA synthesis thiab SOD kev ua haujlwm (Chang et al., 1995) thiab tsis suav cov dawb radicals (Hsu et al., 1995). Txawm li cas los xij, qhovtshuaj sedativenyhuvntawm CD yeej tsis tau kawm.Cov nyhuv sedative ntawm cov tshuaj tuaj yeem soj ntsuam los ntawm kev ntsuas kev ua haujlwm ntawm lub cev muaj zog hauv cov nas (Carpenedo li al., 1994; File and Fernandes, 1994; Hsieh et al., 1991). Yog li, lub hom phiaj ntawm txoj kev tshawb fawb tam sim no yog npaj los ntsuas qhovtshuaj sedativenyhuvntawm CD los ntawm kev ntsuas hexobarbital-induced pw tsaug zog lub sij hawm nyob rau hauv nas thiab locomotorbehavior, nrog rau cov kab rov tav kev ua si, ambulatory lub sij hawm, thiab tag nrho cov nyob deb ntawm nas, ces cais cov CD extract nrog cov kuab tshuaj sib txawv los tshawb nrhiav nws cov khoom xyaw nquag.

2. Cov ntaub ntawv thiab cov txheej txheem

2.1. Kev npaj ntawm CD(Cistanchedeserticola)

Rhizomes ntawmCistanchedeserticolaMa.(Orobanchaceae) tau yuav los ntawm Taiwan lag luam thiab tau lees paub los ntawm Dr. Yung Hsun Chang, Tuam Tshoj Medical College, Taichung.A cov qauv tau muab tso rau hauv Departmentof Botany, China Medical College (identificationno. 15).

Rhizomes ntawm CD(Cistanchedeserticola)(2.5 kg) raug txiav thiab rho tawm nrog 50 feem pua ​​​​ethanol (3 × 3.5 l) los ntawm maceration (3 hnub) thiab cov extract raug txo kom qhuav nrog lub tshuab nqus tsev evaporator. Cov qoob loo ntawm 840g (33.6 feem pua) tau txais. Cov extract tau yaj hauv dej thiab cov tshuaj tau muab rho tawm ua ntu zus nrog ethyl acetate thiab n-butanol (Hsu li al.,1994) kom muab cov seem hauv cov qoob loo ntawm 24.9 g (3.0 feem pua; FrEtOAc,) thiab 81.2 g (9.7 feem pua; FrBuOH ,), raws. Aqueous feem yield yog 242.3 g (28.8 feem pua; FrH2O,). Cov kauj ruam sib cais yog qhia hauv daim duab 1.

Fig. 1. Sib cais ntawm rhizoma ntawmCistanchedeserticola(CD).

2.2. Tsiaj

Txiv neej Sprague-Dawley nas, hnyav ntawm 200-250 g tau siv rau kev kawm txog kev tsav tsheb. Txiv neej ICR nas, qhov hnyav ntawm 20-25g, tau siv rau kev kawm txog cov nyhuv hypnotic. Allanimals tau khaws cia nyob rau hauv pawg ntawm tsib percage ntawm qhov kub-tswj ntawm 21-25 degree thiab av noo ntawm 5595 feem pua. Cov tsiaj tau nyob hauv lub tawb hlau-hlau mesh rau tsawg kawg 7 hnub ua ntej kev sim. Ib qho kev noj zaub mov pellet thiab cov kais dej tau muab tso rau libitum.

2.3. Hexobarbital-induced pw tsaug zog hauv nas

Hauv pab pawg sim, txhua feem ntawm CD(Cistanchedeserticola)cov extracts tau muab tshuaj rau qhov ncauj ntawm ib koob ntawm {{0}}.1,0.3, 1.0 g/kg lub cev qhov hnyav rau ntau pawg ntawm mice. Ib teev tom qab, hexobarbital (100 mg / kg, ip) tau muab los ua kom tsaug zog. Lub caij nyoog ntawm kev poob thiab rov qab los ntawm txoj cai reflex tau siv los ua qhov ntsuas ntawm cov nyhuv hypnotic (Fujimori, 1965). Nyob rau hauv pawg tswj hwm, ib txwm saline siv xwb.

2.4. Kev ntsuas tus kheej lub cev muaj zog acti6ityin nas

Lub Opto-Varimex tsiaj kev ua meter tau raug siv (Columbus Instruments, USA) rau cov kev ntsuas no. Lub apparatus muaj ib tug acrylic kev ua si saib tawb (43.2 × 44.4 × 30.5 cm) uas yog surrounded los ntawm kab rov tav thiab ntsug sensors uas tsis-detectable los ntawm nas.Lub apparatus kuj muaj kaum tsib infraredbeams, spaced 2.65 cm sib nrug ntawm txhua axis. Ob txoj kab hluav taws xob tau teeb tsa raws li qhov rhiab heev ntawm cov neeg saib xyuas.Lub tawb tau muab ntim rau hauv lub txee qhov cua.Kev ntsuas tau ua thaum 09:00–17:00 h.

Kev cuam tshuam ntawm cov kab hluav taws xob photocell tau sau los ntawm microcomputer thiab cov kev hloov pauv hauv qab no tau sau tseg: (1) Kev ua haujlwm kab rov tav (HA): tag nrho cov kev cuam tshuam ntawm kab kab rov tav; (2) lub sij hawm ambulatory (AT): lub sij hawm siv nyob rau hauv ambulatory kev ua si raws li ntsuas los ntawm kev cuam tshuam ntawm cov kab sib txawv; thiab (3) tag nrho qhov nrug (TD): qhov kev ncua deb ntawm tus tsiaj (cm) thaum lub sijhawm txav mus los, raws li ntsuas los ntawm kev cuam tshuam rov qab ntawm tib lub nqaj lossis txav hauv qab ob kab teeb nrog kev so ntawm 1 s, suav nrog kev tu, khawb thiab khawb ( Sanberg et al., 1985). Cov dej num tau kaw rau 1 h, pib 5 feeb tom qab muab tus tsiaj tso rau hauv lub tawb sim. Txhua lub ratwas tsuas yog siv ib zaug thiab tag nrho ntawm rau nas yog siv rau txhua qhov kev kho mob. Txhua feem ntawm CD(Cistanchedeserticola)extract tau muab tshuaj rau qhov ncauj ntawm ib koob ntawm {{0}}.1,0.3, 1.0 g/kg lub cev hnyav rau ntau pawg ntawm cov nas. Ib teev tom qab ntawd, kev ua haujlwm ntawm lub cev muaj zog raug kaw. Tswj nas tau txais tsheb nkaus xwb.

Cistanchedeserticolamuaj ibtshuaj sedativenyhuv

2.5. Tshuaj

Methanol, n-butanol, thiab ethyl acetate yog qib HPLC, tau los ntawm E. Merk.

2.6. Kev txheeb cais

Tag nrho cov ntaub ntawv tau txheeb xyuas cov ntaub ntawv siv ANOVA ua raws li Duncan ntau qhov kev xeem los sib piv lawv qhov sib txawv. PB0.05 yuav tsum muaj rau qhov tseem ceeb ntawm kev txheeb cais.

3. Cov txiaj ntsig

3.1. Hexobarbital-induced pw tsaug zog hauv nas

Raws li qhia hauv Table 1, ethanol extract ntawm CD(Cistanchedeserticola)ntawm {{0}}.1, 0.3, thiab 1.0 g/kg ua rau lub sijhawm pw tsaug zog hexobarbital hauv nas (PB0.01). Nruab nrab ntawm txhua feem ntawm CD(Cistanchedeserticola)extract, tsuas yog cov dej feem ntawm CD rho tawm ntawm {{0}}}01, 0.03, thiab 0.3 g/kgcould prolong the hexobarbital-induced sleeptime in nas (PB0 .01).

3.2. Kev ntsuas tus kheej lub cev muaj zog acti6ityin nas

Raws li qhia hauv daim duab 2, ethanol extract ntawm CD(Cistanchedeserticola)ntawm {{0}}.1,0.3, thiab 1. , thiab totaldistance (PB0.01). Nruab nrab ntawm txhua feem ntawm CD(Cistanchedeserticola)extract, tsuas yog cov dej feem ntawm CD(Cistanchedeserticola)rho tawm ntawm {{0}}. . 3).

Fig. 2. Cov nyhuv ntawm ethanol extract ntawm CD(Cistanchedeserticola)ntawm cov kev hloov hauv spontaneous lub cev muaj zog hauv cov nas. Cov ntaub ntawv yog sawv cev raws li mean9S.EMof rau nas. ANOVA rau kev ntsuas rov qab los ntawm Duncan ntau qhov kev xeem. ** PB0.01, piv nrog rau pawg VEH

Fig. 3. Cov nyhuv ntawm txhua feem ntawm CD(Cistanchedeserticola)rho tawm ntawm cov kev hloov hauv spontaneous lub cev muaj zog ua haujlwm hauv nas. Cov ntaub ntawv yog sawv cev asmean9S.EM ntawm rau nas. ANOVA rau kev ntsuas rov qab los ntawm Duncan ntau qhov kev xeem. ** PB0.01, piv nrog rau pawg VEH.

4. Kev sib tham

Nws tau pom tias ethanol extract ntawm CD(Cistanchedeserticola)tuaj yeem ua kom ntev lub sijhawm hexobarbital-induced pw tsaug zog inmice. Tom qab ntawd cov dej feem ntawm CD(Cistanchedeserticola)Extract tuaj yeem ua kom ntev lub sijhawm hexobarbital-induced tsaug zog ntau dua li lwm feem ntawm CD extract hauv nas. Tsis tas li ntawd, ethanol extracts ntawmCistanchedeserticolaMa. tuaj yeem txo qis txhua qhov kev ua haujlwm ntawm lub cev muaj zog, suav nrog cov kab rov tav, lub sijhawm thauj mus los thiab tag nrho cov nyob deb ntawm cov nas los ntawm qhov tshwm sim hauv daim duab 2. Cov dej feem ntawm CD(Cistanchedeserticola)Extract tuaj yeem tsim qhov txo qis hauv txhua qhov kev ua haujlwm ntawm lub cev muaj zog hauv cov nas ntawm txhua feem ntawm CD(Cistanchedeserticola)rho tawm.

Hauv kev tshawb fawb phytochemical tsis ntev los no, nws tau pom tias cov dej txheej ntawm CD(Cistanchedeserticola)extracts koom nrog cistanoside EI, 8-epideoxylogenic acid, thiab geniposidic acid. Cov neeg koom nrog no muaj ntau yam kev ua haujlwm ntawm lub cev thiab kev ua haujlwm tshuaj (Chinand Chang, 1994). Yog li ntawd, lubtshuaj sedativenyhuvntawm txheej dej ntawm CD(Cistanchedeserticola)extract tej zaum yuav yog qhov kev ua ntawm cistanoside, 8-epideoxylogenic acid, thiab geniposidic acid. Cov sedative constituents ntawm waterlayer ntawm CD extract yuav kawm tom qab.

Hauv kev xaus, CD(Cistanchedeserticola)ethanol extract thiab nws cov waterfraction muaj covtshuaj sedativenyhuv.

Kev lees paub

Kuv thov ua tsaug rau Dr. Ming-TsuenHsieh ntawm lub koom haum ntawm Suav Pharmaceutical Sciences rau nws kev pab.

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