Kev tshawb fawb txog tshuaj lom neeg ntawm Cistanche Deserticola

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Cistanche deserticolayog ib qho tseem ceeb Suav tshuaj ntsuab, uas muaj lub luag haujlwm ntawm Tonifying raum lub zog, muaj txiaj ntsig cov ntsiab lus thiab cov ntshav, moistening hnyuv, thiab lwm yam. Nws decoction thiab cawv extract tuaj yeem txhim kho lub cev tsis muaj zog ntawm Yang deficiency nas (hydrocortisone qauv). Peb tau kawm nws cov tshuaj lom neeg, cais ob lub monomers los ntawm nws cov n-butanol ib feem, txheeb xyuas lawv li 8-epibrucine glucoside thiab mannitol feem. Kaum tsib dawb amino acids raug ntsuas.

Ntsiab lus:Cistanche deserticola amino acid mannitol 8-epibrucine glucoside

Intro

Cistanche deserticolayog ib qho tseem ceeb suav tshuaj ntsuab. Synonymous nrog Cistanche deserticola, goblins, golden xyoob tua, thiab Grand Rue. Feem ntau hu ua "desert ginseng". Nws yog qhov qhuav scaly succulent qia ntawm Cistanche deserticola (Orobanchaceae Cog).

Suav Pharmacopoeia (1) records thekev ua haujlwm ntawm Cistancheyog tonifying raum Yang, muaj txiaj ntsig essence thiab ntshav, moistening plab hnyuv, thiab hais txog. Muaj Cistanche deserticola nyob rau hauv muaj pes tsawg leeg ntawm ntau yam classic tshuaj xws li cov tshuaj Youyin. Yang Guizhen et al. (2) qhia tias Cistanche deserticola Decoction thiab cawv extract tuaj yeem txhim kho kev tiv thaiv kab mob ntawm Yang deficiency nas (prednisone qauv). Cov tshuaj muaj pes tsawg leeg tsis tau qhia. Kobayashi Hiromi et al. (3) tau geraniol glucoside thiab 8-epibrucine Glucoside los ntawm tib lub genus ntawm Cistanche salsa uas tsim nyob rau hauv Inner Mongolia.

Kaum tsib amino acids tau txiav txim los ntawmCistanche deserticola(sau xyoo 1982) hauv Ejina Banner, Gansu Province. Plaub monomers raug rho tawm los ntawm cov txheej txheem hnyav thiab silica gel kem chromatography, ntawm ob lub tebchaw uas tau txheeb xyuas tau yog 8-epibrucine (crystal I) thiab mannitol (crystal II) tau sau tseg raws li hauv qab no.

Kev sim

Lub melting point tester yog Kfloer hom, nuclear magnetic resonance spectrometer: Hom FX-100, H-NMR 100MHz, C-NMR25MHz, huab hwm coj spectrometer: Hom MAT-312, infrared spectrometer: Hom 7650, ultraviolet spectrometer: Hom 710, thiab high-speed amino acid analyzer: Japanese hom 835.

1. Kev rho tawm thiab txheeb xyuas cov siv lead ua I thiab siv lead ua II

Cov hlais ntawm cov ntaub ntawv sim tau muab rho tawm nrog 95 feem pua ethanol nyob rau hauv thermal reflux, thiab cov tshuaj tau evaporated kom tshem tawm cov kuab tshuaj. Cov tshuaj tau muab faib ua plaub qhov hnyav: roj av ether, ether, acetic acid, ethyl cawv, thiab n-butanol. N-butanol ib feem tau raug silica gel kem chromatography, eluted nrog sib txawv proportions ntawmCHCl3-MeOH,EtOH, thiab50 feem pua EtOHCrystal I tau txais los ntawm 1: 1 chcl_3-MeOH eluent, thiab siv lead ua II tau los ntawm50 feem pua EtOHeluent.

Cistanche benefit

Crystal kuv:koob tsis muaj xim (Abs. EtOH) mp 127-128 degree C (tsis raug)

Kev ntsuam xyuas naphthol tau zoo, thiab RF tus nqi ntawm cov pob ntawm cov kua dej hauv niam hydrolyzed tau zoo raws li cov qauv qabzib. Elemental tsom xam: tus nqi sim (%) C46.76, H6.85; tus nqi suav (%) C46.60, H6.84.Raws li cov ntaub ntawv saum toj no, nws zoo ib yam nrog cov ntaub ntawv 8-epibrucine glucoside, yog li nws raug txheeb xyuas tias yog 8-epibrucine.

Crystal II:tsis muaj xim acicular crystal (recrystallization hauvMeOH) mp164-165 degree C (uncorrected) Elemental analysis: kev sim tus nqi (%) C40.29, H7.67; xam tus nqi (%) C39.56, H7.74 IR yog raws li tus qauv infrared spectrum ntawm mannitol; Crystal II yog npaj los ntawm txoj kev ib txwm muaj. Cov xim tsis muaj xim tsis muaj xim (recrystallization hauv ethanol) mp124-125 degree C tau los ntawm hexaacetate ntawm mannitol yog zoo ib yam nrog lub melting point ntawm hexaacetate ntawm mannitol tus qauv, yog li nws raug txheeb xyuas tias yog mannitol.

Cistanche benefit

2. Kev tshuaj xyuas cov amino acids dawb

Noj 10 g ntawmCistanche deserticola(txiav thiab sieved nrog 40 mesh) thiab tshav kub rho nws peb zaug nrog 90 mL ntawm 50 feem pua ethanol thiab lim cov extract. Cov lim dej dhau los ntawm 20g 732 cation txauv resin thiab ntxuav nrog dej distilled kom txog thaum tsis muaj suab thaj hauv cov eluent, thiab ces eluted nrog 20 feem pua NH4OHaqueous daws. Sau cov ndlwg nrog cov tshuaj tiv thaiv zoo rau ninhydrin, mloog zoo rau 10ml, ntxiv sulfosalicylic acid, kho nws mus txog pH 2.2 nrog hydrochloric acid, centrifuge kom tshem tawm cov turbidity, dilute lub clarified tov nrog.0.02NHCl rau 4 times, thiab txiav txim siab nws nrog Japanese Hom 835 high-speed amino acid analyzer.

Various amino acids per hundred grams of Cistanche