Simultaneous HPLC Kev Txiav Txim Ntawm Hydrophilic Whitening Agents hauv Cov Khoom Siv Tshuaj pleev ib ce

Hu rau:joanna.jia@wecistanche.com/ WhatsApp: 008618081934791

Miaw-Ling Chang a, Chur-Min Chang b,*

Abstract

Ib txoj kev ua kua chromatographic ua tau zoo rau kev ntsuas plaub ntawm cov feem ntau siv hydrophilicntxuav hniav dawbtus neeg sawv cev * / glycolic acid (GA), ascorbic acid (AA),arbutin(ART), thiab Mg ascorbyl phosphate (MAP), tau tsim. Kev sib cais isocratic tau ua los ntawm C18 kem nrog cov ion-pair tus neeg sawv cev ua lub sijhawm txawb. Cov analytes tau kuaj pom los ntawm ultraviolet lub teeb nqus ntawm lub wavelength ntawm 220 thiab 240 nm, feem. Calibration curves tau pom tias yog linear hauv 8.0-36 mg/ml (GA), 10.0-300 mg/ml (AA thiab ART), thiab 5.6-451 mg/ml ( MAP). Qhov sib txheeb coefficient ntawm linear regression tsom xam yog nrog qhov ntau 0.9974-0.9997. Kev rov qab los ntawm plaub tus kws tshuaj ntsuam xyuas tau nyob nruab nrab ntawm 94.8 thiab 100.1 feem pua thiab qhov tseeb ntawm txoj kev no tau zoo dua li 6.9 feem pua tus qauv sib txawv (RSD) (n=3). Nws tau pom tias AA degraded hauv cov tshuaj aqueous. Txhawm rau kom paub tseeb tias AA ruaj khov thaum lub sijhawm kev tshuaj xyuasHPLC, tag nrho cov analytes tau yaj hauv cov dej distilled thiab cov kev daws teeb meem no tau purged nrog nitrogen gas tshem tawm oxygen thiab khaws cia ntawm 25 8C. Cov txiaj ntsig kev ntsuam xyuas pom tau tias cov txheej txheem yog qhov ceev, yooj yim, xaiv txoj kev thiab itis haum rau niaj hnub tsom xam ntawm kev lag luam.tshuaj pleev ib ce.# 2003 Elsevier BV All rights reserved.

Cov ntsiab lus: cov khoom siv tshuaj pleev ib ce; Hydrophilic whitening tus neeg sawv cev; Glycolic acid; Ascorbic acid; Arbutin; Mg ascorbyl phosphate; HPLC

Cistanche tshuaj ntsuabyog ib tug natural whitening agent.

1. Taw qhia

Westerners xav txog tanned tawv nqaij noj qab nyob zoo thiab zoo nkauj. Ntawm qhov tsis sib xws, lub teeb ntawm daim tawv nqaij yog suav hais tias zoo nkauj hauv cov teb chaws oriental. Yog li ntawd, whitening lotions yog nrov heev nyob rau hauv theoriental. Lawv yog siv rau whitening ntawm daim tawv nqaij.Chemical peeling feem ntau yog siv nyob rau hauv kev kho mob rau lub ntsej muag puas ntawm daim tawv nqaij tshwm sim los ntawm pob txuv, melasma, ntau warts, thiab hais txog. Hauv cov txheej txheem no,tshuaj pleev ib cecov khoom uas muaj ib tug chemotherapeutic tus neeg saib xyuas yog siv rau ntawm daim tawv nqaij forstimulating lub tshiab ntawm daim tawv nqaij hlwb. Cov xim ntawm daim tawv nqaij yuav sib dua. Yog li no chemo-therapeuticagent tseem hu ua antxuav hniav dawbtus neeg sawv cev [1-6]. Nws tau raug tshaj tawm tias tus neeg sawv cev dawb zoo li glycolic acid (GA), ib qho ntawm hydroxy acids, ua rau kom cov dej ntawm daim tawv nqaij thiab, yog li ntawd, muab cov tsos zoo nkauj dua, ua rau cov kab ntawm lub ntsej muag tsawg dua thiab wrinkles [7,8]. Muaj ob hom tshuaj ntxuav hniav dawb: lipophilic whitening agents thiab hydrophilic agents. Piv txwv ntawm lipophilic whiteningagents yog kojic acid, salicylic acid, ascorbylpalmitate, azelaic acid, thiab adapalene. Piv txwv ntawm hydrophilic whitening agents yog glycolic acid (GA), ascorbic acid (AA),arbutin(ART), thiab magnesium ascorbyl phosphate (MAP). Cov tshuaj ntxuav hniav dawb hydrophilic tau kawm ntawm no vim tias lawv pawg polar muaj kev sib raug zoo tsis zoo rau kab C18 siv hauv HPLC thiab nws nyuaj rau cais lawv hauv HPLC. Peb siv ion-pairmethod los daws qhov teeb meem no.

Cov qauv tshuaj thiab qhov siab tshaj plaws ntawm cov hydrophilicntxuav hniav dawbCov neeg ua haujlwm tau muab rau hauv Table 1. Hauv Taiwan, tsis muaj kev txwv rau qhov concentration ntawm GA thiab AA siv rau hauv cov khoom kom zoo nkauj. ART, txawm li cas los xij, tuaj yeem siv txog li 7 wt. feem pua thiab MAP mus txog 3 wt. feem pua .Txawm hais tias tsis muaj kev txwv rau qhov concentration ntawm GA siv hauv cov khoom siv tshuaj pleev ib ce, nws tau tshaj tawm los ntawm US Food and Drug Administration tias tus neeg sawv cev dawb no tuaj yeem ua rau mob hnyav lossis o ntawm daim tawv nqaij [9]. Feem ntau, txog 30-40 wt. feem pua ntawm GA yog siv nyob rau hauv lubtshuaj pleev ib ceyuav tsum tau siv nyob rau hauv cov kev zoo nkauj salons thiab ib tug ntau dua concentration, 50-70wt. feem pua , yog siv rau hauv cov khoom kom zoo nkauj siv los ntawm cov kws kho mob [10].

Lub hom phiaj ntawm txoj kev tshawb no yog txhawm rau txhim kho aquantitative analysis method kom raug ntsuas cov qib ntawm cov tshuaj ntxuav hniav dawb hauv cov khoom kom zoo nkauj. Txoj haujlwm no tseem ceeb heev rau kev nyab xeeb rau pej xeem. Txawm hais tias muaj ntau cov ntawv tshaj tawm piav qhia txog kev kwv yees ntawm cov whiteners hauv cov qauv tshuaj pleev ib ce [11-16], cov txheej txheem kev soj ntsuam ib txhij tseem tsis tau tshaj tawm hauv cov ntaub ntawv. Qhov kev tshawb fawb no muaj feem xyuam rau txoj kev tshuaj ntsuam xyuas, uas tuaj yeem tshawb pom thiab ua kom muaj nuj nqis ntawm hydrophilic.ntxuav hniav dawbtus neeg sawv cev.

Table 1 Chemical structures of four hydrophilic whitening agents

Lub hydrophilicntxuav hniav dawbCov neeg sawv cev tsis yog ib txwm nyob ruaj khov hauv cov tshuaj aqueous. Lawv raug oxidation. Txawm hais tias muaj ntau qhov kev tshawb fawb txog kev ruaj ntseg ntawm AA tau tshaj tawm [17-19], ib puag ncig ntawm AA hauv cov ntawv ceeb toom no tsis haum rau HPLC rau kev txiav txim siab ib txhij ntawm hydrophilic whiteners. Nws yog ib qho tseem ceeb kom paub tseeb tias cov tshuaj hydrophilic whitening agents ruaj khov thaum lub sij hawm tsom xam rau qhov tseeb ntawm cov txiaj ntsig.

2. Kev sim

2.1. Reagents thiab qauv

GA, AA, ART, thiab MAP thiab cov lag luam tau yuav los ntawm Alfa Co. (Asmeskas), AlfaCo. (Asmeskas), Wako (Japanese), thiab Lipo. Co. (American), feem. Methylparaben thiab U-13 yog tau los ntawm Induchem. Co. (Switzerland). Citric acid, tetrabutylammonium hydroxide (TBAH), sorbitol, phosphoric acid, thiab potassiumdihyrophosphate tau yuav los ntawm AldrichCo. (Asmeskas). Tag nrho cov tshuaj yog analytical reagent qib. Kev lag luamtshuaj pleev ib cecov khoom, los ntawm ntau lub tuam txhab, tau yuav los ntawm cov khw muag khoom thiab los ntawm lub khw muag tshuaj hauv zos.Cov kuab tshuaj thiab dej tau lim los ntawm ib qho 0.45 mmembrane thiab degassed.

2.2. Tswv yim

Lub HPLC apparatus (Hitachi Co., Nyiv) muaj ib tug modular chromatographic system.To uas lub twj tso kua mis (Model I{0}}), UV detector (Model I-7420; variable wavelength), thiab aninjection valve nrog 25 ml qauv voj (Model7725, Rheodyne, Cotati, US) tau txuas. Cov ntaub ntawv tau txais thiab kev ua tiav tau ua tiav nrog lub khoos phis tawj tus kheej siv SISC software (Taiwan).Sample txhaj tshuaj raug cuam tshuam nrog syringe ntawm 25 ml (Hamilton, Switzerland). A 5 mm, 250 mm / 4.60 mm ID A Stainless-steel Mightysil RP-18GPcolumn tau siv.

2.3. Chromatographic tej yam kev mob

Kev tsom xam chromatographic tau ua tiav nrog ib kem isocratic thim rov qab txoj kev. Nyob rau hauv txoj kev ion-pairing, lub sij hawm txawb yog 0.{{10}}}05 M potassium dihydro phosphate buffer tov nrog rau peb cov tshuaj: TBAH, methanol, thiab phosphoric acid. Tus nqi ntawm peb cov tshuaj muaj txiaj ntsig zoo rau kev sib cais thiab kev daws teeb meem ntawm spectrum ntawm HPLC. Theconcentration ntawm TBAH yog varied los ntawm 1 mus rau 10mM; methanol los ntawm 1 mus rau 10 vol. feem pua. Ib tug ntau yam ntawm phosphoric acid tau ntxiv rau lub buffersolution los kho cov pH qhov tseem ceeb ntawm 2.5 mus rau 5.0.Qhov kev ntsuam xyuas yuav tso cai rau ib tug haum mobilephase xaiv rau ib tug zoo daws teeb meem. Allmobile theem tau lim los ntawm Milliporefilter, pore loj 0.45 hli, thiab degassed los ntawm sonication ua ntej yuav siv. Kev khiav dej num ntawm 1.1 ml / min yog siv. Cov analytes tau kuaj pom los ntawm UV nqus ntawm 220 lossis ntawm 240 nm. Tus kheej ntawm plaub cov khoom xyaw tau muab los ntawm chromatography nrog cov qauv tseeb. Quantification yog nqa tawm los ntawm kev sib koom ua ke ntawm lub ncov siv txoj kev externalstandardization.

cov txiaj ntsig cistanchentawmwhitening zoo ntawm daim tawv nqaij

2.4. Calibration nkhaus

Cov kev daws teeb meem tau npaj los ntawm qhov tseeb qhov hnyav ntawm cov neeg sawv cev thiab tom qab ntawd so lawv hauv dej. Tsib qhov kev daws teeb meem ntawm plaub tus kws tshuaj ntsuam xyuas tau npaj tshiab los ntawm lawv cov kev daws teeb meem los ntawm qhov piv ntawm {{0}} nrog dej distilled. Kev tsim nyog dilution ntawm cov kev daws teeb meem ua haujlwm no tau muab cov ntsiab lus ntawm 10 / 300 mg / ml, tshwj tsis yog rau GAand MAP, qhov twg cov concentrations yog 8.0-36.0mg / ml thiab 5.6 / 451 mg / ml, feem. Calibration curves tau tsim los ntawm kev npaj cov peakareas ntawm txhua qhov tivthaiv piv rau cov concentration thiab muab qhov tseem ceeb ntawm txoj kab nqes, nrog rau kev cuam tshuam thiab kev sib txheeb coefficient rau txhua qhov calibrationcurve. Lub calibration curves yog rau kom muaj nuj nqis ntawm plaubntxuav hniav dawbcov neeg sawv cev hauv plaub qauv ntawm kev lag luamtshuaj pleev ib ce.

2.5. Kev kawm rov qab

Ib qho piv txwv cream uas tsis muajntxuav hniav dawbtus neeg sawv cev tau siv los ua kev tswj hwm hauv kev tshawb nrhiav rov qab. Qhov sib txawv ntawm plaub tus neeg sawv cev hydrophilic whitening, GA, AA, ART, thiab MAP, tau ntxiv rau cov tshuaj tiv thaiv no. Cov concentration tau muab rau hauv Table 4. Txog 1 g ntawm txhua tus qauv npaj tau hnyav rau hauv lub khob haus dej thiab 30 ml ntawm cov dej distilled ntxiv thiab tom qab ntawd lub raj mis tau muab tso rau hauv ib da dej ultrasonic rau 15 minthermostatted ntawm 25 8C. Cov kev daws teeb meem tau ua kom txog li 100 ml ntawm cov dej distilled thiab tom qab ntawd tau lim thiab deoxygenated los ntawm N2 rau li 3 min thiab tom qab ntawd kaw nrog Bakelitescrew-cap thiab silicone nplhaib.

3. Cov txiaj ntsig thiab kev sib tham

3.1. Chromatography thiab kev daws teeb meem

Cov qauv ntawm plaubntxuav hniav dawbCov neeg sawv cev, GA, AA, ART, thiab MAP, muaj nyob rau hauv Table 1. Tag nrho cov ntawm lawv yog cov polar molecules heev. Rau cov polar analytes, methanol los yog acetonitrile feem ntau yog siv los ua ib feem ntawm lub xov tooj ntawm tes hauv C18 columnor cyano-propyl kem ntawm HPLC. Txawm li cas los xij, theco-elution ntawm cov polar analytes tau pom thaum siv methanol lossis acetonitrile. Yog li ntawd, nws yog tsis yooj yim sua kom cais cov analytes no. Txhawm rau daws qhov teeb meem no ntawm kev sib koom ua ke, ion-pairing txoj kev tau ua haujlwm los txo lawv cov cim polar kom tau txais lub sijhawm khaws cia. Tsis tas li ntawd, nws yog ib qho tseem ceeb kom nrhiav tau ib qho kev sib cais tsim nyog rau kev soj ntsuam ib txhij ntawm plaub analytes hauvtshuaj pleev ib cecov khoom. Txhawm rau kom tau txais cov xwm txheej zoo tshaj plaws, kev cuam tshuam ntawm lub xov tooj ntawm tes thiab nws cov pH qhov tseem ceeb tau txiav txim siab ntawm no. Cov txiaj ntsig tau txais yog qhia nyob rau hauv daim duab 1. Qhov concentration ntawm TBAH nyob rau hauv lub sij hawm txawb tau txawv ntawm 1 mus rau 10 mM. Lub peev xwm suav tau txo qis raws li qhov concentration ntawm TBAHincreases (saib daim duab 1(a)). Qhov kev poob qis hauv qhov tseem ceeb ntawm qhov muaj peev xwm yog qhov ntse rau MAP tab sis tsis tshua zoo rau GA, ART, thiab AA. Qhov txawv ntawm qhov muaj peev xwm ntawm MAP thiab lwm tus tuaj yeem yog vim muaj zog ioniccharacter ntawm MAP. Qhov sib txawv me me ntawm qhov muaj peev xwm ua rau lub sijhawm khaws cia tsim nyog hauvHPLC. Yog li ntawd, qhov concentration ntawm TBAH raug xaiv los ua 10 mM rau theem mobile. Cov txiaj ntsig ntawm cov tshuaj methanol ntawm lub peev xwm muaj peev xwm tau pom hauv daim duab 1(b). Cov txiaj ntsig zoo sib xws tau pom rau cov rau TBAH. Qhov siab dua ntawm methanol, qhov sib txawv me me ntawm qhov muaj peev xwm, thiab lub sijhawm khaws cia luv dua hauv HPLC. Kaum feem pua ntawm methanol hauv ntim tau raug xaiv hauv qhov no.

Ntau yam ntawm phosphoric acid tau ntxiv rau cov tshuaj tsis tau hais los saum toj no toalter pH qhov tseem ceeb ntawm 2.5 txog 5.0. Ntawm pH tus nqi ntawm 5.0, dav thiab ntau lub ncov tau pom hauv cov txiaj ntsig thechromatograph. Lub chromatograph peaks rau GA, AA, thiab ART yog sib tshooj nrog ib leeg. Tsis zoo li cov txiaj ntsig yav dhau los, qhov muaj peev xwm nce ntxiv nrog qhov nce ntawm pH qhov tseem ceeb rau MAP (saib hauv daim duab 1(c)). Qhov kev hloov pauv ntawm qhov muaj peev xwm vim yog pH qhov tseem ceeb yog me me heev rau GA, AA, thiab ART. Nws yog ib qho tseem ceeb uas yuav tau xaiv ib qho pHvalue uas muab qhov sib txawv txaus ntawm qhov tseem ceeb ntawm qhov muaj peev xwm rau kev daws teeb meem zoo hauv HPLC. Rau cov laj thawj no, tus nqi pH sib npaug li 2.5 tau raug xaiv. Raws li qhov tshwm sim, lub sijhawm tsim nyog rau HPLC kev tshuaj xyuas yog ib qho 0.005 M potassiumdihydrophosphate buffer tov uas muaj 10mM TBAH, 10 vol. feem pua ntawm methanol, thiab phosphoric acid. Cov phosphoric acid tau siv los kho tus nqi pH ntawm cov tshuaj tsis haum rau 2.5.

figure 1 + figure 2

3.2. Kev txiav txim siab wavelength rau UV ntes

UV spectrum ntawm nontxuav hniav dawbCov neeg ua hauj lwm dissolved nyob rau hauv lub mobile theem tau txais los ntawm kev siv lub UV spectrophotometer. Qhov siab tshaj plaws nqus dej siab tshaj plaws yog nyob ntawm 220 nm rau GA, ntawm 243 nm forAA, ntawm 227 nm rau ART, thiab ntawm 238 nm rau MAP.At 240 nm, UV ntes muaj qhov siab tshaj plaws hauv kev kuaj AA thiab MAP, tab sis qis heev rau GA thiab ART (saib daim duab 2(b)).Thaum nyob ntawm 220 nm, cov peaks ntshiab hauv HPLCchromatograph tau pom rau tag nrho cov plaub hau dawb (saib daim duab 2(a)). Txhawm rau kom tau txais txiaj ntsig zoo dua rau tag nrho cov analytes, lub wavelength ntawm tus ntes tau hloov kho mus rau 220 nm rau kev tshawb nrhiav thiab kom muaj nuj nqis ntawm plaub analytes. Cov txheej txheem yog qhov nrawm nrog lub sijhawm ntsuas ntsuas ntawm 12.1 min ntawm chav tsev kub (kwv yees 26 8C).

Table 3 Effect of temperatures, oxygen, and pH values on the stability of AA

3.3. Kev tshawb fawb ruaj khov rau cov tshuaj ntxuav hniav dawb

Lub AA tau decomposition rau dehydroascorbic acid (DHA) thaum yaj hauv dej. Yog li ntawd, nws yog ib qho tseem ceeb kom ua kom cov ascobic acid ruaj khov thaum lub sij hawm tsom xam rau qhov tseeb ntawm cov txiaj ntsig. Fernandes et al. qhia tias lub ntsiab tseem ceeb cuam tshuam rau qhov degradation yog oxygen thiab kub [19]. Hauv lawv txoj kev tshawb fawb, kev degradation yog qhov tseem ceeb hauv 1 h, thiab cov txiaj ntsig ntawm pH qhov tseem ceeb ntawm kev daws teeb meem ntawm kev degradation tsuas yog hais txog pH 5. AA hauv ntau qhov sib txawv ntawm ib puag ncig xws li kev kho lossis tsis kho nrog N2, pH tus nqi thiab qhov kub thiab txias. Lub sijhawm ntev rau kev hloov pauv tseem ceeb, qhov ruaj khov ascorbic ntau dua.

Cov txiaj ntsig, suav nrog hauv Table 3, ua kom pom kev sib raug zoo ntawm kev ruaj ntseg thiab kev kho tsis zoo nrog N2. Kev poob ntawm AA cov tshuaj tsis kho yog sai dua li cov tshuaj kho. Raws li pom nyob rau hauv Table 3, ntawm 10 8C thiab pH2, lub sij hawm yuav tsum tau rau 10 feem pua degradation ntawm theAA yog li 10 h tsis kho thiab txog 48h rau cov tshuaj kho. Qhov no tej zaum yuav yog vim tus yam ntxwv uas muaj oxygen tsawg dua muaj rau oxidation. Nws raug sau tseg tias, nyob rau hauv muaj cov pa oxygen thiab ntawm 25 8C, qib ntawm AA ntawm 8.7 thiab 71.2 feem pua tom qab 10 teev raug soj ntsuam rau pH 6.9 thiab 2.0. Qhov txiaj ntsig no tuaj yeem piav qhia los ntawm qhov ntau dua ntawm qhov tsis sib xws ntawm AA ntawm pH 6.9 thiab daim ntawv no yuav tsum tsis ruaj khov dua li daim ntawv tsis sib xws [19]. Ntawm qhov tsis sib xws, thaum tsis muaj oxygen thiab ntawm 25 lossis 10 8C, cov kua dej ntawm AA yog qhov zoo dua ntawm pH 6.9. Qhov laj thawj rau AA kom ruaj khov dua tseem tsis tau tshawb xyuas ntxiv. Kev txheeb xyuas qhov kub thiab txias, nyob rau hauv untreatedsolutions, AA tsis ruaj khov ntawm qhov kub thiab txias ({25}}C) dua li ntawm qhov kub qis (10 8C).Txawm li cas los xij, rau cov kev daws teeb meem, cov tshuaj aqueous ntawm AA Nws ruaj khov thiab tsis cuam tshuam los ntawm qhov kub thiab txias.

3.4. Validation rau linearity thiab ntsuam xyuas rau precision

Siv cov xwm txheej chromatographic tau piav qhia, qhov kev daws teeb meem tsim nyog tau ua tiav ntawm cov analytes thiab lwm cov tebchaw hauvtshuaj pleev ib cekhoom. Cov txheej txheem tau validated rau linearity thiab precision. Linear nkhaus haum yog siv los xam cov calibration curves rau txhua tusntxuav hniav dawbtus neeg sawv cev. Cov txiaj ntsig tau muab rau hauv Table 4.Excellent linearity tau txais ntau tshaj li ntawm 5.6 txog 451 mg / ml rau txhua tus qauv tshwj tsis yog rau GA uas qhov ntau yog los ntawm 8 txog 36 mg / ml.Cov coefficient sib txuas yog nyob ntawm 0 .9974 rau 0.9997. Qhov tseeb ntawm txoj kev raug xam raws li qhov txheeb ze tus qauv sib txawv (RSD, n{{10}}) ntawm cov kev soj ntsuam uas muaj cov plaub hydrophilic whiteners nyob rau tib qhov ntau ntawm cov concentration. RSD ntau yam tau pom los ntawm 0.3 txog 6.43 feem pua.

Table 4 Validation data from calibration curves of four hydrophilic whitening agents

3.5. Rov qab

Ib tug cream uas tsis muajntxuav hniav dawbcov neeg sawv cev tau siv los ua kev tswj hwm hauv kev tshawb nrhiav kev rov qab los. Cov me me ntawm plaub tus neeg ua kom dawb huv tau ntxiv rau cov tshuaj tiv thaiv no. HPLC chromatography qhia tau hais tias tsis muaj UV absorption peakdetected rau ib tug tswj cream (saib daim duab. 3(a)) thiab plaub lub ncov nqus tau pom rau ib tug samplecream uas muaj plaub cov tshuaj ntxuav hniav dawb. InFig. 3(b), ncov 1 yog lub siab nqus UV rauGA; ncov 2 rau AA; ncov 3 rau ART; ncov 4 forMAP. Txhua tus neeg sawv cev dawb muaj nws lub sijhawm khaws cia. Cov cim no yuav raug siv los txheeb xyuas cov neeg ua kom tawv nqaij tom qab. Cov txiaj ntsig tau muab sau tseg rau hauv Table 2. Qhov ntau ntawm 94-100 feem pua rov qab los rau plaub tus neeg sawv cev tau txais ntawm ob qho tib si qis thiab siab. Cov coefficients ntawm variance xam los ntawm peb replicates yog tag nrho cov tsawg tshaj li 6.9 feem pua .Lub HPLC chromatograms ntawm plaub analytes muab rho tawm los ntawm cov kev sim formulation tau qhia nyob rau hauv daim duab 3 thiab tsis muaj kev cuam tshuam.

Cistanche has anti-aging, antioxidant, and skincare effects

cistanche cogmuajanti-aging, antioxidant, thiabcov teebmeem ntawm daim tawv nqaij

3.6. Daim ntawv thov

Plaub muag muajtshuaj pleev ib cecov khoom raug soj ntsuam. Lawv yogntxuav hniav dawbcream (cosmetic number 1), C20 whitening gel (cosmeticnumber 2), essence lotion (cosmetic number 3), and tev lotion (cosmetic number 4), feem. Cov chromatographs forthe plaub tshuaj pleev ib ce yog muab nyob rau hauv daim duab 4(a / d).Chromatograph 4 (a) muab ib tug me me absorptionpeak ntawm ncov 4, piv txwv li cosmetic naj npawb 1 muajMAP. Chromatograph 4 (b) nthuav tawm lub ncov ntawm peak 2, uas yog ib qho kev qhia tias cosmeticnumber 2 muaj AA. Los ntawm chromatograph 4 (c) thiab (d), ART tau txheeb pom nyob rau hauv cosmeticnumber 3 thiab ob qho tib si GA thiab AA tau txheeb xyuas nyob rau hauv cosmetic tooj 4. Muaj ib qho tsis muaj cim chromatograph 4 (d). Qhov no UV absorption peakhas lub sijhawm khaws cia ntev dua li lub ncov 3 tab sis luv dua li lub ncov 4. Nws yog qhov tseeb tias qhov tsis pom qhov nqus dej no tsis yog vim cov neeg ua kom tawv nqaij, tab sis vim yog lwm cov khoom xyaw incosmetic 4. Qhov ntau ntawm cov tshuaj dawb no tau ua los ntawm cov Kev koom ua ke ntawm cov ncov hauv chromatography siv cov txheej txheem sab nraud. Qib ntawm cov neeg ua kom tawv nqaij dawb hauv plaub notshuaj pleev ib cetau muab rau hauv kab lus 5. Nws tau sau tseg tias cov txiaj ntsig tau lees paub qhov tseeb thiab qhia tias ua raws li daim ntawv thov.

4. Cov lus xaus

Kev ntsuam xyuas HPLC tau tsim yog yooj yim thiab nrawm rau kev soj ntsuam ib txhij ntawm hydrophilicntxuav hniav dawbtus neeg sawv cev. Lub peev xwm muaj peev xwm tau siv los xaiv lub sijhawm tsim nyog rau HPLC. Weneed xaiv ib qho xwm txheej uas muab qhov sib txawv me me ntawm qhov muaj peev xwm ua tau rau lub sijhawm tuav tsim nyog. Nws yog ib qho tseem ceeb rau qhov kev xaiv kom muab qhov sib txawv txaus ntawm qhov tseem ceeb ntawm lub peev xwm rau kev daws teeb meem zoo hauv thechromatograph ntawm HPLC. Raws li qhov tshwm sim, lub sijhawm tsim nyog rau HPLC kev tshuaj xyuas yog ib qho 0.005 Mpotassium dihydrophosphate buffer tov uas muaj 10 mM TBAH, 10 vol. feem pua ntawm methanol, thiab phosphoric acid. Cov phosphoric acid tau siv los kho tus nqi pH ntawm cov tshuaj tsis haum rau 2.5. Abetter nrhiav wavelength rau lubntxuav hniav dawbCov neeg sawv cev yog nyob ntawm 220 nm rau HPLC. Tsis tas li ntawd, txoj kev yooj yim rau stabilizing AA hauv cov txheej txheem analytical tau txais. Uas yog ib qho tseem ceeb rau qhov tseeb ntawm cov txiaj ntsig.