Kev tshawb fawb Status Ntawm Pharmacology Thiab Tshuaj Discovery Of Cistanche hauv Nco
Mar 23, 2023
TSAB NTAWV
Qhov xwm txheej ntawm cov kab mob neurodegenerative ntev tau nce, tab sis tsis muaj kev kho mob zoo vim tsis muaj ntaub ntawv hais txog cov txheej txheem pathological ntawm cov kab mob no. Yog li, qhov kev tshawb fawb no tau tshawb xyuas qhov cuam tshuam ntawmcistancheextract ntawm kev txo qis kev nco thiab kev hloov pauv neurobiochemical hauv streptozotocin dementia nas. Cov nas (n¼35) tau muab tso rau 5 pawg ntawm 7 nas rau ib pab pawg; Pab pawg 1: tswj pawg nas tau muab cov tshuaj ib leeg; Pawg 2: streptozotocin; Pawg III: Streptozotocin ntxiv 100 mg/kgcistancherho tawm; Pawg IV: Streptozotocin þ myrtenal 200 mg/kg; Pawg V: Streptozotocin ntxiv rau Donepezil 0.5 mg / kg. Cov ntaub ntawv los ntawm txoj kev tshawb no qhia tias kev tswj hwm streptozotocin (STZ) cuam tshuam lub cim xeeb ntawm cov nas thiab ua rau muaj kev hloov pauv hauv cov cim ntawm oxidative inflammatory teb, cholinergic muaj nuj nqi, cell qauv, thiab qib neurotransmitter. Tshwj xeeb, kev tswj hwm ntawmcistancheextract tiv thaiv lub hlwb los ntawm oxidative inflammatory kev nyuaj siab los ntawm kev ua kom cov kev ua ntawm endogenous antioxidant enzymes, li no pab txhawb spatial nco kev ua tau zoo nyob rau hauv stz dementia nas. Kev kho mob nrog cistanche kuj rov qab ua haujlwm cholinergic thiab stabilized neurotransmitter homeostasis hauv cov nas dementia.CistancheTej zaum yuav muaj txiaj ntsig zoo hauv kev kho cov neeg mob uas muaj dementia cuam tshuam nrog Alzheimer's kab mob, cov kws sau ntawv theorize.
Ntsiab lus: Cistanche; Nco; Alzheimer tus kab mob; Streptozotocin
1. Taw qhia
Ntau cov kev tshawb fawb niaj hnub qhia txog qhov nce ntawm dementia tus nqi thoob ntiaj teb. Ntawm ntau hom kev dementia uas tau txheeb pom, Alzheimer's hom dementia (DAT) yog suav tias yog qhov hnyav tshaj plaws, cuam tshuam txog 6 feem pua ntawm cov poj niam thiab 5 feem pua ntawm cov txiv neej hnub nyoog 60 xyoo. Tsis tas li ntawd, kev tshawb fawb los ntawm Cov Neeg Laus Demographics. thiab Memory Study (ADAMS) kwv yees tias kwv yees li 4.5 lab tus neeg Asmeskas muaj dementia, tus lej uas tuaj yeem nce mus txog 16 lab los ntawm 2050.
Pathologically, Alzheimer's tus kab mob yog tus cwj pwm los ntawm cov deposition ntawm neurofibrillary tangles, tsub zuj zuj ntawm amyloid beta peptides, thiab hyperphosphorylated tau proteins txuam nrog microtubules. Cov yam ntxwv zoo li no tau cuam tshuam nrog neuronal thiab synaptic poob thiab qhia txog cov kev kho mob niaj hnub no uas lub hom phiaj txhawm rau txhim kho kev ua haujlwm ntawm cov neurons noj qab haus huv yam tsis muaj kev cuam tshuam kev loj hlob ntawm cov hlwb neuronal. Tam sim no tsis muaj kev kho mob kom thim rov qab cov yam ntxwv neuropathological ntawm cov txheej txheem kab mob, ib feem vim qhov tshwj xeeb ua rau Alzheimer's hom dementia (DAT) tsis tau txheeb xyuas thiab cov kev taw qhia txoj hauv kev nyuaj. Cov kev tshawb fawb tsis ntev los no tau pom tias oxidative kev nyuaj siab thiab lipid peroxidation, uas yog nce nyob rau hauv txoj kev loj hlob ntawm DAT, kuj txo lub paj hlwb siv, neuroinflammation, thiab tej yam uas impair cholinergic neurotransmission. Nyob rau hauv txoj kab nrog cov kev xav no, peb xav hais tias lub hom phiaj oxidative kev nyuaj siab thiab txhim kho lub xeev antioxidant ntawm lub hlwb yog cov tswv yim tseem ceeb los txo cov kab mob pathophysiology thiab txhim kho kev nkag siab niaj hnub ntawm Alzheimer's kab mob-hom dementia.
Yees duab: Faw Cistanche
Txawm li cas los xij, tsis tau muaj kev tshawb fawb me me ntawm cov tshuaj antioxidants uas tuaj yeem txo qis cov kab mob pathophysiology thiab neurobiochemical hloov pauv cuam tshuam nrog kev nco poob hauv Alzheimer's disease. Nws yog ib qho tseem ceeb kom nco ntsoov tias cov khoom antioxidant ntawm cistanche tseem yuav raug tshawb xyuas. Phenylethanolsides nyob rau hauv cistanche yog cov organic tebchaw uas feem ntau muab rho tawm los ntawm ntau cov nroj tsuag tseem ceeb roj. Cov tshuaj no muaj ntau yam kev ua haujlwm ntawm lub cev, suav nrog cov tshuaj tiv thaiv kab mob antioxidant, tiv thaiv kab mob, tiv thaiv apoptotic thiab tiv thaiv qog ua haujlwm.
Txawm hais tias, hauv kev sim nas cov qauv ntawm Parkinson tus kab mob, phenylethanol sab ntseeg tau tias muaj cov teebmeem neuroprotective, tsis muaj ntaub ntawv qhia txog lawv qhov kev nco-txhim kho lub peev xwm thiab lawv cov txiaj ntsig kho hauv kev txhim kho DAT induced los ntawm STZ txoj kev kho. Yog li, txoj kev tshawb fawb tam sim no tau tsim los tsim tus qauv nas ntawm kev dementia siv streptozotocin thiab tshawb xyuas qhov muaj peev xwm ntawm miti ntawm pathophysiology ntawm kev nco tsis nco thiab kev hloov pauv neurobiochemical hauv kev siv myrtle.
2. Cov ntaub ntawv thiab tau ntsibhods
2.1. Tsiaj txhu
Cov neeg laus txiv neej albino nas tau siv hauv txoj kev tshawb no. Cov nas tau nyob hauv lub tawb yas ntawm chav tsev kub (22 2 C) hauv cov tsiaj chaw, raug rau 12- teev lub teeb / tsaus ntuj, thiab muaj kev nkag mus pub dawb thiab dej. Cov nas raug kuaj xyuas kev noj qab haus huv thiab tsis muaj kab mob thoob plaws lub sijhawm kawm, thiab kev ntxhov siab thaum lub sijhawm sim tau txo qis los ntawm kev ua kom muaj kev tuav tsawg. Tag nrho cov tsiaj raug tso cai rau acclimate mus kuaj mob rau tsib hnub ua ntej kev sim coj cwj pwm.
2.2. Dosage thiab kev tswj hwm txoj kev
Donepezil (DP) hmoov tau yaj nyob rau hauv {{0}}}.9 feem pua ntawm cov ntsev ib txwm thiab muab tshuaj ntawm 0.5 mg/kg (0.9 feem pua w/v). Streptozootocin tau yaj hauv cov kua cerebrospinal fluid tshiab (aCSF) ua tus nqa khoom thiab muab tshuaj ntawm 3mg / kg nrog 10 μL icv ntawm txhua sab. Cov pab pawg tswj hwm tau txais kev txhaj tshuaj simulated nrog cov kua dej cerebrospinal rau thawj hnub thiab thib peb ntawm kev tswj hwm streptozotocin. Cov khoom xyaw nquag ntawm cistanche extract tau yaj nyob rau hauv pob kws roj thiab hais lus ntawm koob tshuaj 100mg / kg thiab 200mg / kg, thaum pawg tswj tau muab 10ml / kg pob kws roj. Dosing ntawmcistancheraug xaiv raws li LD50 cov ntaub ntawv dhau los. Txhua yam tshuaj yog npaj tshiab ua ntej siv.
2.3. Kev sim dementia tshwm sim los ntawm streptozotocin
Nyob rau thawj hnub thiab thib peb, dementia raug ntxias los ntawm kev txhaj tshuaj intraventricular ntawm ob koob tshuaj sib txawv ntawm streptotoxomycin. Nyob rau hauv luv luv, nyob rau hauv cov tshuaj loog, kev siv cov khoom stereotactic raws li cov nram qab no ventricular coordinates nrog ob tog intubation ntawm tsiaj: ua ntej - tom qab lub quarter 0.8; Nruab nrab sab nraud ¼1.5; Poob rov qab plab 3.4. Cov nas hauv pawg tswj hwm tau txais kev txhaj tshuaj intraventricular ntawm cov kua cerebrospinal.
2.4. Kev sim tsim
Cov nas tau muab faib ua tsib pawg nrog rau 7 nas nyob rau hauv txhua pab pawg thiab muab tshuaj raws li kev noj tshuaj uas qhia hauv Table 1: pretreated nrogcistancherau 7 hnub ua ntej thawj zaug kev tswj hwm streptozotocin thiab txuas ntxiv rau 14 hnub tom qab ntawd. Donepezil thiab myrtenal tau noj 30 feeb ua ntej txhaj tshuaj streptozotocin.
2.5. Kev ntsuas peev xwm nco
Kev ua tau zoo ntawm kev nco tau raug ntsuas los ntawm kev sim Y-maze tau piav qhia ua ntej. Qhov kev sim no tau siv los txheeb xyuas qhov hloov pauv ntawm tus nas tus cwj pwm raws li qhov qhia txog kev nco lub sijhawm luv. Chav ntsuas no muaj peb lub caj npab zoo ib yam, sau "A," "B," thiab "C," thiab muab txhua tus nas nyob rau txhua lub sijhawm ntawm "tsev" caj npab sau A. Cov nas tau tso cai tshawb nrhiav qhov tshawb nrhiav. unlimited tsib feeb. Lwm qhov kev ua tau zoo tau raug kaw raws li kev nkag mus rau hauv tag nrho peb caj npab. Tom qab ua tiav txhua theem kev ntsuam xyuas, so txhua chav nrog 75 feem pua ethanol kom tsis txhob muaj ntxhiab tsw.
3. Cov cim ntawm lub hlwb oxidative kev nyuaj siab
3.1. Kev txiav txim siab ntawm cerebral lipid peroxidation
Raws li yav dhau los tau piav los ntawm Ohkawa li al., cov concentration ntawm malondialdehyde (MDA), cov khoom ntawm lipid peroxidation, nyob rau hauv hippocampal secretion emulsion yog txiav txim los ntawm spectrophotometric. Qhov kev hloov ntawm absorbance yog ntsuas los ntawm spectrophotometric ntawm 532 nm.
3.2. Kev txiav txim siab ntawm nitric oxide (NO) concentration hauv hlwb
Qhov concentration ntawm NO yog txiav txim los ntawm kev sim koob tshuaj calorimetry. Cov tshuaj tiv thaiv yog raws li kev kwv yees ntawm nitrite tsim. Tom qab ntawd, qhov nqus tau nyeem ntawm 548 nm.
3.3. Cov qog nqaij hlav necrosis tau siv los ua tus cim los ntsuas qhov kev mob ntawm qhov mob. Qhov concentration ntawm TNF- hauv hippocampus tau txiav txim siab siv tus nas TNF- ELISA cov khoom siv ntawm MyBio Source (USA) No. 4. Ntawm 540 nm, microplate nyeem ntawv tau siv rau kev nyeem ntawv spectrophotometric.
3.4. Rau kev tshem tawm cov protein, cov ntaub so ntswg hippocampal tau homogenized nyob rau hauv qhov txias extraction tsis, ces cov qauv homogenized tov yog centrifuged ntawm 10, 000 rpm ntawm 4 degree C rau 5 min, thiab cov protein ntau npaum li cas tau ntsuas los ntawm kev kuaj xyuas cov protein. cov khoom siv raws li cov neeg tsim khoom cov lus qhia.
3.5. Kev ntsuam xyuas ntawm Brain acetylcholinesterase (AChE) kev ua acetylcholinesterase kev ua hauv hippocampus tau kwv yees raws li txoj kev piav qhia los ntawm Ellman. Cov xim pom tau raug nyeem los ntawm spectrophotometric ntawm 412 nm thiab ACHE kev ua tau ntsuas raws li tus qauv nkhaus hauv μM / SH pawg (MSH).
3.6. Cov ntsiab lus ntawm cov cim antioxidant, glutamyl sulfide peroxidase (GPx), superoxide dismutase (SOD) thiab catalase (CAT) hauv cov ntaub so ntswg hippocampal tau txiav txim los ntawm MyBioSource (USA) cata logue No. 2 enzyme-linked immunosorbent assay.
Daim duab: Cistanches Benefits
4. Cov txiaj ntsig
4.1. Hauv kev sim Y tshawb nrhiav, streptozotocin ua rau kev ua haujlwm tsis zoo ntawm lub cim xeeb tau txo qis (p < 0 01), qhov hloov pauv feem pua ntawm kev txhim kho sau tseg hauv pab pawg kho mob (0.001, raws li) attenuated streptozotocin induced memory impairment. piv rau pawg tswj hwm qhov tseem ceeb. Txoj kev tshawb no tau pom cov txiaj ntsig ntawm cistanche kho nrog streptozotocin ntawm autonomic txav ntawm nas nyob rau hauv qhib teb. Streptozotocin txo qis kev ua haujlwm ntawm lub cev muaj zog piv nrog rau pawg tswj hwm. Txawm li cas los xij, kho nrogcistancheextract (100 thiab 200 mg/kg, po) yog qhov tseem ceeb (p < 0.01 thiab p < 0.001, raws li) thim rov qab cov nyhuv piv rau pawg streptozotocin, uas muaj lub cev ua haujlwm tsawg tshaj plaws. Tom qab kev kho mob streptozotocin, cov teebmeem ntawmcistanche extractntawm kev noj cov nas tau piav qhia los ntawm cov lej suav ntsug, thiab kev noj zaub mov ntawm streptozotocin cov nas kho tau poob qis dua piv nrog cov pab pawg tswj. Txawm li cas los xij, kev kho mob nrog cistanche extract (100 thiab 200 mg / kg, po) yog qhov tseem ceeb (p < 0.05, p < 0.01, raws li) nce tus naj npawb ntawm ntsug, thaum streptozotocin (streptozotocin). ) cov tsiaj kho mob muaj tsawg tshaj plaws ntsug suav.
4.2. Cov txiaj ntsig ntawm Myrtenal kev tswj hwm ntawm kev nce siab nitric oxide concentration hauv hippocampal cov ntaub so ntswg ntawm cov nas raug streptozotocin. Cov txiaj ntsig tau pom tias 200 mg / kg cistanche extract txo qis streptozotocin dementia nas (p
Pic: Cov teebmeem ntawm cistanche txhim kho kev nco
5. Kev sib tham
Hauv kev kawm tam sim no, peb tsom kom nkag siab zoo duacistanche covKev kho lub luag haujlwm hauv dementia, tshwj xeeb yog Alzheimer's disease. Peb tau siv streptozotocin lub peev xwm los tsim cov qauv kev sim ntawm dementia thiab ua kom pom cistanche lub peev xwm los txo cov neurodegenerative deficits qauv hauv kev sim qauv ntawm Alzheimer's syndrome hom (DAT). Ib qho txiaj ntsig tseem ceeb ntawm Cistanche yog txhim kho kev nco tsis zoo, uas tuaj yeem raug ntaus nqi rau nws cov khoom tiv thaiv hyperglycemic, nce qib qabzib thiab siv hauv nruab nrab paj hlwb. Cov txiaj ntsig no tau lees paub cov kev tshawb fawb yav dhau los uas tau pom tias muaj kev txhim kho tseem ceeb hauv kev paub txog kev ua haujlwm tom qab cistanche tau muab rau cov qauv nas ntawm kev ntxhov siab thiab kev nco tsis tau tshwm sim los ntawm kev txhaj tshuaj scopolamine. Cov ntaub ntawv pov thawj qhia tias cistanche, ua tus neeg sawv cev kho mob, ua lub luag haujlwm tseem ceeb hauv kev txo cov txheej txheem neurodegenerative uas ua rau nco tsis nco qab hauv dementia. Txawm li cas los xij, ua pov thawj tag nrho qhov kev xav no yuav tsum muaj kev sim nrog lwm cov tshuaj cholinergic uas ua rau kev dementia.
Nyem qhov no txhawm rau txhim kho koj lub cim xeeb
6. Cov lus xaus
Cov ntaub ntawv los ntawm txoj kev tshawb no pom tau hais tias streptozootocin siv impaired spatial nco nyob rau hauv nas thiab induced hloov nyob rau hauv cov cim ntawm oxidative inflammatory teb, cholinergic muaj nuj nqi, cov ntaub so ntswg qauv, thiab theem ntawm neurotransmitters nyob rau hauv lub hippocampus. Txawm li cas los xij, cistanche extract tiv thaiv lub hlwb los ntawm oxidative inflammatory kev nyuaj siab los ntawm kev ua kom cov kev ua ntawm endogenous antioxidant enzymes, yog li pab txhawb spatial nco kev ua tau zoo nyob rau hauv streptomycin dementia nas. Ntxiv rau, peb qhia tias kev kho mob nrogcistancherestores cholinergic muaj nuj nqi thiab stabilizes neurotransmitter homeostasis nyob rau hauv nas kho nrog streptozotocin nyob rau hauv dementia. Raws li lub latitude ntawm neurobiochemical yam xav tau hauv txoj kev tshawb fawb tam sim no, cov kws sau ntawv xaus lus tias cistanche ua lub luag haujlwm tseem ceeb hauv kev tswj cov cim cim xeeb. Yog li nws pom tau tias noj cistanche tuaj yeem ua tau zoo hauv kev kho cov neeg uas muaj Alzheimer's-txog dementias.
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