Rov Los Ntawm Innate Immune Cells Thiab Persisting Alterations in Adaptive Immunity in The Peripheral Blood Of Convalescent Plasma Donors Thaum Yim Hli Tom Qab SARS-CoV-2 Kab Mob Part 2

3.4. Kev hloov pauv hauv Kev Tiv Thaiv Kev Tiv Thaiv Kab Mob Tseem Ceeb nyob rau yim hli tom qab kis kab mob

Nyob rau hauv sib piv rau innate caj npab ntawm kev tiv thaiv, peb cov ntaub ntawv muab pov thawj ntawm ib co persisting activation ntawm caj npab adaptive txawm nyob rau yim lub hlis tom qab-SARS-CoV-2 kab mob. Nws yog qhov pom tau tias feem pua ​​​​ntawm tag nrho cov B hlwb tsis rov qab mus rau qib qub txawm tias tom qab yim lub hlis (qhov feem pua ​​​​-18.7 feem pua ​​​​hauv kev tswj hwm 13.1 feem pua ​​​​thiab 12.7 feem pua ​​​​hauv CP pub dawb ntawm ob lub hlis thiab yim lub hlis tom qab kis kab mob, p < 0.001), txawm tias lymphopenia tau hloov pauv tag nrho ntawm ob lub hlis tom qab kis kab mob.

Lub cev tiv thaiv kab mob hauv lub cev yog lub cev lub cev tiv thaiv kab mob tshaj plaws. Nws ua haujlwm txij thaum yug los los tiv thaiv peb ntawm cov kab mob thiab kab mob. Qhov no yog qhov sib txawv ntawm lub cev tiv thaiv kab mob, uas tsuas yog ncaws thaum kis kab mob lossis kab mob thiab siv sijhawm los tsim kev tiv thaiv kab mob.

Lub cev tiv thaiv kab mob hauv lub cev muaj xws li daim tawv nqaij, mucous daim nyias nyias, macrophages, thiab platelets, thiab lwm yam, uas pab tiv thaiv lub cev los ntawm kev kis kab mob thiab lwm yam aggressors. Qhov no yog vim li cas peb xav tias qee zaum lub cev yuav kov yeej tus kab mob ntawm nws tus kheej yam tsis muaj kev cuam tshuam sab nraud. Cov neeg uas muaj zog tiv thaiv kab mob hauv lub cev feem ntau zoo dua tuaj yeem tiv nrog ntau yam mob.

Txawm li cas los xij, lub cev tiv thaiv kab mob hauv lub cev tsis zoo tag nrho thiab tsis tuaj yeem tiv thaiv txhua tus kab mob thiab kab mob. Lub sijhawm no, lub cev tiv thaiv kab mob tau txais lub luag haujlwm, uas pab lub cev tsim cov tshuaj tiv thaiv tshwj xeeb thiab tawm tsam ntau yam kab mob phem. Qhov kev tiv thaiv tau muaj zog dua, lub cev tiv taus ntau dua thiab tuaj yeem tshem tawm cov kab mob thiab co toxins sai dua thiab zoo dua. Txhawm rau kom muaj lub cev tiv thaiv kab mob noj qab haus huv, ntxiv rau kev tawm dag zog txaus, noj zaub mov zoo, thiab pw tsaug zog txaus, peb kuj yuav tsum tau ua tib zoo saib xyuas peb lub siab. Kev ntxhov siab thiab kev ntxhov siab txo qis kev tiv thaiv thiab ua rau lub cev muaj kab mob. Ntsuas lub siab xav thiab tswj tus cwj pwm zoo siab tuaj yeem txhim kho lub cev tiv thaiv kab mob thiab pab txhawb kev noj qab haus huv ntawm lub cev mus ntev.

Kev noj zaub mov kom raug kuj tseem ceeb heev hauv kev txhim kho peb lub cev tiv thaiv kab mob. Kev noj zaub mov tuaj yeem muab cov khoom noj txaus rau lub cev thiab tshem tawm cov co toxins ntawm lub cev. Tshwj xeeb tshaj yog qab zib thiab cov zaub mov ua kom zoo, cov khoom noj no nyiam ua kom lub cev noj qab haus huv. Kev noj zaub mov ntau dua, xws li zaub, txiv hmab txiv ntoo, nplej tag nrho, txiv ntoo, ntses, thiab lwm yam, tuaj yeem muab lub cev nrog ntau yam khoom noj uas tseem ceeb thiab pab txhawb kev tiv thaiv kab mob.

Feem ntau, kev koom tes ntawm kev tiv thaiv kab mob hauv lub cev thiab kev tiv thaiv kab mob tuaj yeem txhim kho peb lub cev tiv thaiv. Nyob rau lub sijhawm ntev, nws tuaj yeem pab koj tswj koj txoj kev noj qab haus huv thiab tso cai rau koj kom muaj lub neej zoo dua. Nco ntsoov, txhua tus tuaj yeem txhim kho lawv txoj kev tiv thaiv thiab tswj kev noj qab haus huv los ntawm kev coj ua lub neej zoo, cia peb sib txhawb. Los ntawm qhov kev xav no, peb yuav tsum txhim kho kev tiv thaiv. Cistanche tuaj yeem txhim kho kev tiv thaiv zoo. Cistanche yog nplua nuj nyob rau hauv ntau yam tshuaj antioxidant, xws li vitamin C, vitamins, carotenoids, thiab lwm yam. Cov khoom xyaw no tuaj yeem tshem tawm cov dawb radicals, txo oxidative kev nyuaj siab, thiab txhim kho kev tiv thaiv. tiv thaiv kab mob.

Nyem cistanche tubulosa cov txiaj ntsig

Hais txog CD4 ntxiv rau T cell compartment, peb qhov kev txheeb xyuas yav dhau los tau qhia txog kev kho tag nrho ntawm COVID-19- cuam tshuam txog CD4 polarization ntawm ob lub hlis tom qab kis kab mob [13], tshwj tsis yog qee tus neeg mob uas muaj ntau dua ntawm Th{ {5}} thiab Th17- hom subsets. CP cov neeg pub dawb 'Th cells tau sim nyob rau yim lub hlis khaws cov kev rov qab los ntawm Th1 / Th2 piv, qhov feem pua ​​​​ntawm Th17- thiab Th{10}} hom hlwb poob qis, sensibly vim qhov txwv tsis pub siv ntxiv ntawm qhov no hom polarized tiv thaiv kab mob.

Ntawm qhov tsis sib xws, peb cov kev soj ntsuam tau pom muaj kev hloov pauv tseem ceeb ntawm yim lub hlis tom qab kis kab mob mus rau qhov txo qis CD4 / CD8 piv (txhais tau tias CD4 / CD8 piv—4.5 hauv kev tswj hwm thiab 2.2 ntawm ob thiab yim lub hlis, tom qab kis kab mob, p < { {10}}.01) thiab qhov cim txo qis ntawm CD8 ntxiv rau Tregs uas tseem qis tsis tu ncua, txij li pib kis kab mob. Tsis tas li ntawd, NKT hlwb thiab tshwj xeeb tshaj yog lawv cov CD56 ntxiv rau CD16- qhov chaw tau pom muaj ntau ntxiv txawm tias nyob rau yim lub hlis tom qab-COVID-19 kab mob.

Ntawm qhov kev ceeb toom, feem pua ​​​​ntawm CD56 ntxiv rau CD16- NKT compartment tau pom qhov nce ntxiv ntawm ob- thiab yim lub hlis ntsuas cov ntsiab lus (qhov feem pua ​​​​-6 feem pua ​​​​vs. 7.1 feem pua ​​​​ntawm tag nrho CD3 ntxiv cov hlwb ntawm ob thiab yim lub hlis, raws li, p=0.002).

4. Kev sib tham

COVID-19 muaj feem cuam tshuam nrog kev tiv thaiv kab mob tshwj xeeb, thiab ntau qhov kev tshawb fawb tau tshaj tawm txog kev hloov pauv hauv kev tiv thaiv kab mob tom qab SARS-CoV-2 lub cev tiv thaiv kab mob [4,9,10,15]. Txawm li cas los xij, feem ntau ntawm cov kev tshawb fawb no tau tsom mus rau cov kev hloov pauv thaum lub sij hawm muaj tus kab mob COVID-19 thiab/lossis nyob rau lub sijhawm luv luv. Hauv txoj kev tshawb fawb tam sim no, peb tshaj tawm peb cov txiaj ntsig ntawm qhov kev ntsuam xyuas mus sij hawm ntev ntawm kev tiv thaiv kab mob ntawm CP cov neeg pub dawb, uas yav dhau los tau kis tus kab mob SARS-CoV-2 thiab tau teeb tsa cov lus teb humoral, raws li tau lees paub los ntawm kev tsim cov tshuaj tiv thaiv tshwj xeeb. tawm tsam S1-cov protein ntawm tus kab mob.

Peb cov txiaj ntsig tau pom tias qee qhov kev hloov pauv, tshwj xeeb hauv cov lymphocyte subsets ntawm kev hloov kho caj npab ntawm kev tiv thaiv kab mob, tseem nyob hauv yim lub hlis tom qab kis kab mob. Peb kuj tau pom tias tib lub sijhawm, cov feem pua ​​​​ntawm kev tiv thaiv kab mob hauv lub cev loj (monocytes, granulocytes, thiab NK hlwb), feem ntau ntawm cov phenotypically txawv subsets, yog tib yam lossis zoo ib yam li cov qib kev noj qab haus huv, qhia tias cov Kev daws teeb meem ntawm cov kab mob inflammatory zoo li yuav txuas nrog qhov hloov pauv ntawm cov neeg nruab nrab ntawm cov neeg nruab nrab soluble (chemokine thiab cytokine) - tsim cov hlwb.

Ua ntej, txawm hais tias feem pua ​​​​ntawm tag nrho cov monocytes hauv CP cov neeg pub dawb ntawm yim lub hlis tau nce ntxiv piv rau ob lub hlis thiab kev tswj hwm qhov tseem ceeb, peb cov ntaub ntawv tau hais txog cov kinetics tshwj xeeb ntawm qhov sib txawv monocytic subsets tom qab COVID-19 kab mob.

Hauv kev nthuav dav ntxiv, yav dhau los peb tau tshaj tawm txog qhov tsis txaus ntseeg ntawm tag nrho cov pej xeem monocyte hauv PB ntawm cov neeg mob COVID-19 nquag, pom zoo vim yog kev nrhiav neeg ua haujlwm ntawm macrophages rau lub ntsws lossis lwm qhov chaw muaj mob [13]. Hauv kev pom zoo nrog lwm cov kev tshawb fawb, peb kuj tau pom qhov pom tseeb nce hauv nruab nrab (CD14 ntxiv rau CD16 ntxiv) monocyte subset thiab qhov nce siab tsawg dua hauv cov khoom tsis yog classic (CD14-CD16 ntxiv) ntawm tus nqi ntawm classical monocytes (CD14 ntxiv rau CD16-) ​​thaum lub sijhawm ua haujlwm ntawm COVID-19 theem [13,16,17].

Lub luag hauj lwm ntawm nruab nrab monocytes tsis yog tag nrho elucidated, txawm hais tias lawv paub tias yog cov tshuaj tiv thaiv kab mob uas koom nrog kev loj hlob thiab kev txhawb nqa ntawm T hlwb nrog tsim kev koom tes hauv cov lus teb los tiv thaiv kab mob [18-20]. Lawv muaj peev xwm zais GM-CSF, TNF- , thiab IL-6, uas yog ib qho tseem ceeb rau inflammatory cytokine cua daj cua dub los ntawm COVID-19 cov neeg mob; yog li ntawd, cov feem pua ​​​​siab dua ntawm cov txheej txheem no tau cuam tshuam nrog kev mob ntsws hnyav, kev nkag mus rau ICU, thiab nce lub sijhawm tawm hauv tsev kho mob [16,21]. Cov monocytes uas tsis yog classical yog cov xov tooj ntawm tes nrog kev saib xyuas tus cwj pwm hauv endothelium, uas tseem tuaj yeem cuam tshuam rau cov lus teb los tiv thaiv kab mob [22].

Lawv qhov tshwj xeeb phenotypic kos npe converges rau ib qho kev tiv thaiv kab mob ntawm kev ua, thaum cov pov thawj tshiab txhawb lawv lub peev xwm mus rau hauv lub zos hloov dua siab tshiab rau hauv lwm yam activated macrophages [22,23]. Hauv peb pawg, peb pom tias feem pua ​​​​ntawm cov monocytes nruab nrab tau rov qab los sai sai (ntawm ob lub hlis) tom qab kis kab mob, hais txog lawv lub luag haujlwm tseem ceeb thaum lub sijhawm mob hnyav ntawm COVID-19.

Ntawm qhov tsis sib xws, kev rov qab los ntawm cov monocytes uas tsis yog classical tau ncua sijhawm thiab tau pom tseeb ntawm yim lub hlis, qhia txog kev tiv thaiv kab mob ntev ntev ntev li ob lub hlis tom qab kis kab mob, ntxiv dag zog rau lawv txoj kev daws teeb meem ntawm qhov mob.

Peb cov txiaj ntsig tau pom zoo nrog Neeland li al., uas tsis ntev los no tau tshaj tawm cov npe monocyte zoo sib xws hauv CP cov neeg laus nyob rau xya lub lis piam tom qab kis kab mob, nrog rau feem pua ​​​​ntawm tag nrho cov monocytes thiab qhov tsis muaj txiaj ntsig tseem ceeb hauv tib lub monocyte subsets raws li kev soj ntsuam ntawm no. [12]. Nws tseem yuav tsum tau muab sau tseg tias nyob rau hauv peb kev soj ntsuam, tag nrho cov monocytes tau gated ntawm WBCs, thiab yog li, tej zaum yuav muaj xws li phenotypes uas tsis tas yuav qhia cov cim uas siv los txheeb xyuas cov subsets tau hais los saum no (xws li, CD14- tsis paub tab monocytes).

Nthuav kinetics kuj tau pom nyob rau hauv granulocytes subsets uas pom ib tug maj mam rov qab los nyob rau hauv lub sij hawm, txawm hais tias feem pua ​​ntawm tag nrho cov granulocytes nyob rau yim lub hlis tau txo me ntsis piv rau ob lub hlis tus nqi.

Raws li yav dhau los tau qhia [13], feem pua ​​​​ntawm tag nrho cov granulocytes tau nce thaum lub sij hawm muaj kev tiv thaiv COVID-19 nrog kev nce ntau ntawm lawv cov CD11b ntxiv rau cov khoom siv hluav taws xob thiab kev txo qis ntawm CD11b- feem. Tom qab ob lub hlis tom qab kis kab mob, feem pua ​​​​ntawm CD11b ntxiv rau granulocytes tseem nyob siab hauv CP cov neeg pub dawb (txhais tau tias -72.3 feem pua ​​​​ntawm tag nrho cov granulocytes hauv cov neeg mob COVID-19 vs. 64,5 feem pua ​​​​hauv CP pub ob lub hlis tom qab kis kab mob vs. 46.1 feem pua ​​​​ntawm cov neeg pub noj qab haus huv), yog li muab pov thawj ntxiv ntawm kev tiv thaiv kab mob tsis tu ncua.

Thaum yim lub hlis tom qab kis kab mob, qhov ntau ntawm CD11b ntxiv rau cov kab mob tau txo qis, ua rau CD11b ntxiv / CD11b- granulocyte piv zoo ib yam li cov neeg noj qab haus huv ib txwm muaj. CD11b, ib qho protein subunit ntawm heterodimeric amb2, yog ib qho chaw sib xyaw ua ke ntawm ob peb leukocytes uas sib haum nrog lwm cov cell migration, phagocytosis, chemotaxis, thiab cell-mediated cytotoxicity. CD11b immunophenotyping tau siv los cais cov kab mob tiv thaiv kab mob sib txawv hauv cov txheej txheem mob ntsws ntsws [24], qhov twg, hauv COVID-19, peb thiab lwm tus tau hais txog qhov sib cuam tshuam ncaj qha ntawm qhov nce CD11b ntxiv / CD11b- piv rau cov tsos mob tsis zoo [ 13, 25] ib.

Ntxiv mus, yav dhau los peb tau pom tias cov CP cov neeg pub dawb uas tsis tsim SARS-CoV-2 cov tshuaj tiv thaiv txawm tias lawv qhov kev sim PCR-zoo muaj qhov nruab nrab ntawm CD11b ntxiv rau granulocytes, qis dua piv rau CP cov neeg pub dawb nrog cov tshuaj tiv thaiv kab mob thiab piv rau cov kev tswj tsis tau cuam tshuam, ib qho kev soj ntsuam uas txuas ntxiv nrog qhov mob hnyav ntawm tus kab mob nrog hyperinflammation thiab muaj zog tiv thaiv kab mob [26].

Raws li peb cov tshuaj tiv thaiv kab mob tsis suav nrog cov cim tshwj xeeb uas yuav paub qhov txawv ntawm neutrophils los ntawm lwm cov granulocytes (eosinophils, basophils, mast cells), qhov txo qis ntawm lawv feem pua ​​​​ntawm yim lub hlis piv rau ob lub hlis tuaj yeem yog vim qhov feem pua ​​​​ntawm cov subsets hauv PB. Hauv kev txhawb nqa, tus naj npawb ntawm eosinophils tau pom tias txo qis thaum lub sijhawm CP [12], thaum lub tshuab ua haujlwm mast hlwb uas cuam tshuam rau kev txhim kho cov mob fibrotic thaum lub sijhawm ua haujlwm ntawm COVID-19, kuj tseem yuav cia siab tias yuav txo qis tom qab kev daws teeb meem. ntawm lub qhov rooj [27]

Qhov feem pua ​​​​ntawm NK hlwb ntawm yim lub hlis kuj zoo ib yam li cov qib kev noj qab haus huv. Qhov no yog qhov tseem ceeb vim tias NK hlwb yog qhov tseem ceeb rau kev tiv thaiv kab mob tiv thaiv kab mob thiab lawv cov kev ua haujlwm muaj txiaj ntsig yog thawj kab ntawm kev tiv thaiv uas txwv cov kab mob sib kis. Yog li, kev kho dua tshiab ntawm lawv cov qib mus rau qhov qub qhia tau tias muaj kev daws teeb meem tiav ntawm kev kis tus kab mob ntawm yim lub hlis. Qhov qis qis hauv CD56-CD16 ntxiv rau NK cell subset, phenotype characterizing lub cim xeeb zoo li cov hlwb, ntxiv rau kev daws teeb meem ntawm tus kab mob kis [28].

Cov lus hais rov qab los ntawm cov monocytes, granulocytes, thiab NK hlwb hauv PB ntawm COVID-19- rov qab tau CP cov neeg pub dawb yim lub hlis tom qab kis kab mob tuaj yeem kwv yees tau. Viral clearance feem ntau yog ib qho kev ua haujlwm ntawm lub cev tiv thaiv kab mob hauv lub cev, tau ntsib nrog cov kinetics sai ntawm kev tshem tawm tus kab mob.

Txawm li cas los xij, profileing ntawm lub cev tiv thaiv kab mob hloov pauv tsis zoo li ua raws li tus qauv qub. Peb yav dhau los tau tshaj tawm txog kev hloov pauv tseem ceeb hauv kev hloov pauv ntawm cov cell subsets thaum lub sijhawm ua haujlwm ntawm COVID-19 theem, uas cuam tshuam rau feem pua ​​​​ntawm cov hlwb B, T hlwb, thiab NKT hlwb vim qhov pom tseeb lymphopenia uas ua rau COVID-19, thiab raws li cuam tshuam cov khoom cuam tshuam ntawm lawv ntau yam kev tiv thaiv kab mob.

Ua ntej, tag nrho cov B hlwb raug txo kom tsawg thoob plaws lub sijhawm kawm thiab kuaj pom txawm tias nyob rau yim lub hlis tom qab kis kab mob. Txawm hais tias peb lub vaj huam sib luag tsis suav nrog cov cim tshwj xeeb B ntawm tes rau kev ntsuas qhov tob B cell subset, qhov txheeb cais tau nce Th2- hom hlwb thaum lub sijhawm ua haujlwm ntawm COVID-19 ua ke nrog cov lej ntawm B hlwb raug txo kom tsawg. imply ib tug skewed piv rau antibody-secreting effector B hlwb thiab tej zaum terminally txawv thiab heev secreting plasma hlwb [10].

Qhov kev pheej hmoo ntawm B cell deficiency nyob rau yim lub hlis tom qab tus kab mob COVID-19 yuav qhia tau tias B cell qaug zog nyob rau hauv ib puag ncig uas xav tau sij hawm ntau dua los ua kom tag nrho; Nws kuj tseem tuaj yeem yog qhov tshwm sim ntawm qhov chaw hyperinflammatory (IFN- siab) thaum lub sijhawm ua haujlwm ntawm COVID-19 tau tshaj tawm tias muaj kev cuam tshuam tsis zoo rau B cell txoj kev loj hlob [29]. Txawm li cas los xij, txij li cov neeg pub nyiaj CP suav nrog hauv txoj kev tshawb fawb tau tsim cov qib tshuaj tiv thaiv-SARS-CoV-2 cov tshuaj tiv thaiv, yuav tsum muaj cov ncauj lus kom ntxaws B cell vaj huam sib luag los piav qhia cov laj thawj rau B cell puas.

Cov tib neeg Convalescent qhia tau hais tias muaj zog thiab tej zaum yuav muaj lub cim xeeb tiv thaiv kab mob los ntawm CD4 ntxiv thiab CD8 ntxiv rau T hlwb [10,30,31]. Hauv peb txoj kev tshawb fawb, feem ntau ntawm cov neeg pub dawb CP tau pom ntau feem pua ​​​​ntawm CD8 ntxiv rau T hlwb ob qho tib si ntawm ob thiab yim lub hlis tom qab kis kab mob, uas, ua ke nrog cov qib qis ntawm CD8 ntxiv rau Tregs, tej zaum yuav qhia tau tias muaj kev tiv thaiv kab mob cytotoxic tiv thaiv tau. SARS-CoV2 residuals.

Kev txhawb nqa ntxiv ntawm qhov kev xav no yog los ntawm qhov tseeb tias NKT hlwb thiab tshwj xeeb tshaj yog CD56 ntxiv rau CD16- qhov chaw tau pom muaj ntau ntxiv nyob rau ob lub hlis [13] thiab yim lub hlis tom qab kis kab mob. CD3 ntxiv rau CD56 ntxiv rau NKT hlwb qhia NK thiab T cell cov yam ntxwv thiab tau tshaj tawm tias tau raug suav hais tias yog tus neeg nruab nrab nruab nrab ntawm lub cev thiab kev tiv thaiv kev tiv thaiv nrog lub luag haujlwm tseem ceeb hauv cov lus teb los tiv thaiv kab mob [32,33]. NKT hlwb, txawm hais tias pom muaj tsawg heev hauv PB, tsim cov qib siab ntawm IFN-, tswj qhov sib npaug ntawm Th1 / Th2 hlwb, thiab txhim kho CD4 ntxiv thiab CD8 ntxiv rau T cell ua haujlwm, hos thaum depleted hauv vivo, viral replication zoo heev. .

Hauv COVID-19, nce qib ntawm NKT hlwb tau pom feem ntau hauv cov mob me me thiab tau thov los txhawb kev tswj hwm tus kab mob sai, yog ncaj qha cytotoxic lossis kho cov tshuaj tiv thaiv kab mob ntawm cov cell-mediated cytotoxic teebmeem [34]. Yog li ntawd, qhov muaj feem tseem ceeb ntawm NKT hlwb txog li yim lub hlis tom qab kis kab mob tuaj yeem, ntawm ib sab, txhawb cov pov thawj tias tus kab mob inflammatory (piv txwv li, tej zaum yuav muaj qee cov kab mob tsis zoo) tsis tau tshem tawm tag nrho, tab sis ntawm qhov tod tes. , kuj tseem tuaj yeem qhia txog NKT cell-mediated cytotoxic activation uas tseem ceeb toom thiab tej zaum yuav them rov qab rau lub luag haujlwm ntawm kev rov qab los rau-ze li qub NK thiab CD8 ntxiv rau T hlwb.
Hais txog cov kev txwv ntawm peb txoj kev tshawb fawb, peb yuav tsum nco ntsoov tias qhov kev ntsuam xyuas ntawm cov khoom ntawm lub cev tiv thaiv kab mob tiv thaiv SARS-CoV-2 yog ntawm cov kev tshawb fawb theem nrab hauv cov ntsiab lus ntawm theem 2 kev soj ntsuam ntawm CP. Yog li, nws tuaj yeem ua tsis tau raws li qhov tseem ceeb ntawm cov koom haum tseem ceeb hauv kev tshuaj ntsuam xyuas pawg. Tsis tas li ntawd, tag nrho cov kev ntsuam xyuas tau ua nyob rau hauv cov qauv PB los ntawm cov neeg koom. Txij li COVID-19 feem ntau yog kab mob pulmonary, cov qauv alveolar tuaj yeem muab cov ntsiab lus tseeb dua ntawm cov tshuaj tiv thaiv kab mob tsim tawm.

5. Cov lus xaus

Hauv kev xaus, CP cov neeg pub nyiaj tam sim no muaj qhov tshwj xeeb tiv thaiv kab mob nyob rau yim lub hlis tom qab-COVID-19 tus kab mob, uas yog tus cwj pwm los ntawm kev kho kom rov qab los ntawm cov khoom hauv lub cev tiv thaiv kab mob nrog rau kev tsis sib haum xeeb ntawm SARS-CoV-2 ntawm adaptive immunity. Elucidating lub luv- thiab ntev lub cev tiv thaiv kab mob hloov pauv rau SARS-CoV-2 yog qhov tseem ceeb ntawm kev kho mob, kev tiv thaiv, thiab tsim cov tswv yim txhaj tshuaj zoo.

Txawm hais tias lub cev tiv thaiv kab mob mus ntev tom qab txhaj tshuaj tiv thaiv tseem yuav raug txiav txim siab, qhov kev hloov pauv hloov pauv hloov pauv kev tiv thaiv kab mob tom qab COVID-19 zoo li yuav txhawb nqa qhov txiaj ntsig ntawm kev txhaj tshuaj. Cov tshuaj tiv thaiv lub hom phiaj tiv thaiv kab mob tiv thaiv kab mob SARS-CoV-2 sai dua li kev kis kab mob. Neutralizing cov tshuaj tiv thaiv kab mob thiab SARS-CoV-2- cov lus teb tshwj xeeb B thiab T cell yuav tiv thaiv tus kab mob los yog tsawg kawg tswj tus kab mob tam sim los tiv thaiv kab mob hnyav.

Tus sau kev koom tes:

Conceptualization, OET thiab ET; methodology, TSIS-S., PR, IVK, IPT, OET; software, PR, IVK; validation, IN-S., ​​EK, MG, IC, IPT, ET; kev soj ntsuam, IVK, NO-S.; kev tshawb nrhiav, TSIS-S., EK, OET, CP, IN-S., ​​IC, EK, MG, ET; cov peev txheej, M.-AD, OET, ET; cov ntaub ntawv curation, PR, EK, ET; kev sau ntawv—kev npaj ua ntej, IVK, NO-S.; sau— tshuaj xyuas thiab kho, IN-S., ​​ET, IC, OET, EK, EK, MG, IPT, M.-AD; visualization, TSIS-S., IVK; kev saib xyuas, M.-AD, OET, ET; kev tswj xyuas qhov project, PR, CP, EK, OET, ET; kev nrhiav nyiaj txiag, ET, M.-AD Txhua tus kws sau ntawv tau nyeem thiab pom zoo rau cov ntawv luam tawm ntawm cov ntawv sau.

Nyiaj txiag:

Qhov kev tshawb fawb no yog ib feem nyiaj los ntawm SYN-ENOSIS (17315 / SARG-NKUA).

Institutional Review Board Statement:

Txoj kev tshawb no tau ua raws li cov lus qhia ntawm Kev Tshaj Tawm ntawm Helsinki, thiab pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Ncaj Ncees) ntawm "Alexandra" General Tsev Kho Mob, Athens, Tim Nkij teb chaws (txoj cai code 245, 16 Plaub Hlis 2020).

Cov Lus Qhia Txog Kev Pom Zoo:

Cov ntaub ntawv pom zoo tau txais los ntawm txhua yam kev koom tes hauv txoj kev tshawb fawb.

Cov ntaub ntawv muaj nyob:

Cov ntaub ntawv muaj nyob rau ntawm kev thov los ntawm cov kws sau ntawv.

Kev tsis sib haum xeeb ntawm kev txaus siab:

Cov kws sau ntawv tshaj tawm tsis muaj kev sib cav txog kev txaus siab.

Cov ntaub ntawv

1.Tsitsilonis, OE; Paraskevis, D.; Lianidou, E.; Pierros, V.; Akalestos, UA; Kastritis, E.; Moutsatsou, P.; Scorilas, UA; Sphicopoulos, T.; Terpos, E.; ua al. Seroprevalence of Antibodies tiv thaiv SARS-CoV-2 ntawm Cov Neeg Ua Haujlwm thiab Cov Tub Ntxhais Kawm ntawm National thiab Kapodistrian University of Athens, Greece: Daim Ntawv Qhia Ua Ntej. Lub neej 2020, 10, 214. [CrossRef]

2. Gavriatopoulo, M.; Korompoki, E.; Fotou, D.; Ntanasis-Stathopoulos, I.; Psaltopoulo, T.; Kastritis, E.; Terpos, E.; Dimopoulos, MA Organ-specific manifestations of COVID-19 kab mob. Clin. Exp. Med. 2020, 20, 493–506. [CrossRef]

1. 3. Shi, Y.; Wang, Y.; Chaw, C.; Huang, J.; Yog, J.; Huang, X.; Bucci, E.; Piacentini, M. Ib, G.; Melino, G. COVID-19 tus kab mob: Cov kev xav ntawm kev tiv thaiv kab mob. Cell tuag txawv. 2020, 27, 1451–1454. [CrossRef] [PubMed]

4. Cao, X. COVID-19: Immunopathology thiab nws qhov cuam tshuam rau kev kho. Nat. Rev. Immunol. 2020, 20, 269–270. [CrossRef]

5. Jamilloux, Y.; Henry, T.; Taub, A.; Viel, S.; Faus, M.; El Jammal, T. Walzer, T.; Francois, B.; Seve, P. Peb puas yuav tsum txhawb lossis txhawb nqa lub cev tiv thaiv kab mob rau COVID-19? Cytokine thiab anti-cytokine cuam tshuam. Autoimmun. Rev. 2020, 19, 102567. [CrossRef] [PubMed]

6. Haag, S.; Jylhava, J.; Wang, Y.; Xu, H.; Metzner, C.; Annetorp, M.; ib. Garcia-Ptacek, S.; Khedri, M.; Bostrom, AM; Kadir, UA; ua al. Hnub nyoog, Frailty, thiab Comorbidity li Prognostic Factors for Short-term Outcomes in Patients With Coronavirus Disease 2019 in Geriatric Care. J. Am. Med. Dir. Assoc. 2020, 21, 1555–1559.e2. [CrossRef] [PubMed]

7. Wendel Garcia, PD; Fumeaux, T.; Guerci, P.; Heuberger, DM; Montomoli, J.; Roche-Campo, F.; Schuepbach, RA; Hilty, MP; Cov kws tshawb nrhiav, R.-I. Cov xwm txheej tshwm sim muaj feem cuam tshuam nrog kev pheej hmoo tuag thiab kev kis kab mob hauv 639 cov neeg mob hnyav nrog COVID-19 hauv Tebchaws Europe: Cov ntawv ceeb toom thawj zaug ntawm RISC thoob ntiaj teb-19-ICU cov neeg soj ntsuam yav tom ntej. EClinicalMedicine 2020, 25, 100449. [CrossRef] [PubMed]

8. Zhou, Z.; Rau, L.; Zhang, L. Zhou, J.; Xiao, Y.; Jia, Z.; Guo, L.; Yaj, J.; Wang, C.; Jiang, S.; ua al. Heightened Innate Immune Responses in the Respiratory Tract of COVID-19 Cov neeg mob. Cell Host Microbe 2020, 27, 883–890.e2. [CrossRef]

9. Seb, A.; Crotty, S. Adaptive immunity to SARS-CoV-2 and COVID-19. Cell 2021, 184, 861–880. [CrossRef]

10. Dan, JM; Mas, J.; Kato, Y.; Hasti, KM; Yus, ED; Faliti, CE; Grifoni, UA; Ramirez, SI; Hawb, S.; Frazier, UA; ua al. Immunological memory rau SARS-CoV-2 soj ntsuam txog li 8 lub hlis tom qab kis kab mob. Science 2021, 371, eabf4063. [CrossRef] [PubMed]

11. Breton, G.; Mendoza, P. Hagglof, T.; Oliveira, TY; Schaefer-Babajew, D.; Gaebler, C.; Taub, M.; Hurley, UA; Caskey, M.; Nussenzweig, MC Persistent cellular immunity to SARS-CoV-2 infection. J. Exp. Med. 2021, 218, e20202515. [CrossRef]

12. Neeland, MR; Bannister, S.; Clifford, V.; Daws, K.; Mulholland, K.; Sutton, P. Curtis, N.; Steer, AC; Burgner, DP; Crawford, NW; ua al. Innate cell profiles thaum lub sij hawm mob thiab convalescent theem ntawm SARS-CoV-2 kab mob nyob rau hauv cov me nyuam. Nat. Pawg. 2021, 12, 1084. [CrossRef]

13. Orologas-Stavrou, N.; Politou, M. Rousakis, P.; Kostopoulos, IV; Ntanasis-Stathopoulos, I.; Yaj, E.; Tsiligkeridou, E.; Gavriatopoulo, M.; Kastritis, E.; Kotanidou, UA; ua al. Peripheral Blood Immune Profiling of Convalescent Plasma Donors Reveals Alterations in Specific Immune Subpopulations even at 2 months Post SARS-CoV-2 Infection. Kab mob 2020, 13, 26. [CrossRef]

14. Terpos, E.; Politou, M. Sergentanis, TN; Mentis, A.; Rosati, M.; Stellas, D.; Taub, J.; Hu, X.; Felber, BK; Papa, V.; ua al. Anti-SARS-CoV-2 Antibody Responses in Convalescent Plasma Donors tau nce hauv cov neeg mob hauv tsev kho mob; Subanalyses ntawm Phase 2 Clinical Study. Cov kab mob microorganisms 2020, 8, 1885. [CrossRef]

15. Rezaei, M.; Marjani, M.; Mahmudi, S.; Mortaz, E.; Mansouri, D. Dynamic Changes of Lymphocyte Subsets in Course of COVID-19. Int. Arch. Kev tsis haum tshuaj Immunol. 2021, 182, 254–262. [CrossRef]

16. Zhang, D.; Guo, R.; Li, L.; Li, H.; Wang, Y.; Wang, Y.; Qian, H.; Dai, T.; Zhang, T. Laj, Y.; ua al. COVID-19 kev kis kab mob ua rau pom tau yooj yim nrhiav tau morphologic thiab inflammatory-txog phenotypic hloov pauv hauv peripheral ntshav monocytes. J. Leukoc. Biol. 2021, 109, 13–22. [CrossRef] [PubMed]

17. Laing, AG; Lorenc, UA; Del Molino Del Barrio, I.; Das, A.; Ntses, M.; Monin, L.; Muñoz-Ruiz, M.; McKenzie, DR; Hayday, TS; Francos-Qijorna, I.; ua al. Kev pom zoo Covid-19 kos npe tiv thaiv kab mob sib xyaw ua ke tiv thaiv kev tiv thaiv nrog kev sib cais zoo li tus kab mob sepsis uas cuam tshuam nrog kev ua tsis zoo. medRxiv 2020. [CrossRef]

18. Kwissa, M.; Nakaya, HI; Onlamoon, N.; Wrammert, J.; Villinger, F.; ib. Perng, GC; Yog, S.; Pattanapananyasat, K.; Chokephaibulkit, K.; Ahmed, R. ua al. Tus kab mob dengue ua rau muaj kev nthuav dav ntawm CD14 (ntxiv rau ) CD16 (ntxiv rau) cov pej xeem monocyte uas txhawb kev sib txawv ntawm plasmablast. Cell Host Microbe 2014, 16, 115–127. [CrossRef] [PubMed]

19. Merad, M.; Martin, JC Pathological o hauv cov neeg mob nrog COVID-19: Lub luag haujlwm tseem ceeb rau monocytes thiab macrophages. Nat. Rev. Immunol. 2020, 20, 355–362. [CrossRef] [PubMed]

20. Sampath, P.; Moideen, K.; Ranganathan, UD; Bethunaickan, R. Monocyte Subsets: Phenotypes thiab Function in Tuberculosis Infection. Pem hauv ntej. Immunol. 2018, 9, 1726. [CrossRef] [PubMed]

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