Quercetin Ua Tus Nephroprotector Tom Qab Sov Ischemia: Kev Ntsuam Xyuas Histomorphometric hauv Tus Qauv Naj Npawb
Mar 20, 2022
Gabriela F. Yuav Gonçalves1, Maria Eduarda M. Silva2, Francisco JB Sampaio1, Marco A. Pereira-Sampaio1, 3, Diogo Benchimol de Souza1
TSAB NTAWV
Lub hom phiaj:Txhawm rau ntsuas ntau qhov kev tiv thaiv ntev ntev ntawm quercetin thaum lub raum sov ischemia.
Cov ntaub ntawv thiab cov txheej txheem:Txiv neej nas raug faib ua 4 pawg: sham (S), sham quercetin (SQ), ischemia (I), thiab ischemia quercetin (IQ). Pawg SQ thiab IQ tau txais quercetin (50mg / kg) ua ntej thiab tom qab phais. Pawg kuv thiab IQ tau lawv cov hlab ntsha ntawm lub raum sab laug clamped rau 60 feeb. Txhua tus tsiaj tau euthanized plaub lub lis piam tom qab tus txheej txheem, thiab cov ntshav urea thiab creatinine tau ntsuas. Lub raum hnyav thiab ntim, cortex-non-cortex cheeb tsam ratio (C-NC), cortical ntim (CV), glomerular volumetric ntom (Vv [glom]), ntim-qhov hnyav glomerular ntim (VWGV), thiab ib tug xov tooj ntawm glomeruli ibraum(N [glom]) raug soj ntsuam los ntawm txoj kev stereological. Cov txiaj ntsig tau suav hais tias yog qhov tseem ceeb thaum p<>
Cov txiaj ntsig:Serum urea qib hauv pab pawg kuv tau nce 10.4 feem pua ntawm cov pab pawg S, tab sis tsis muaj qhov sib txawv ntawm lwm pab pawg. C-NC ntawm pab pawg kuv qis dua li ntawm tag nrho lwm pab pawg, thiab pab pawg IQ muaj txiaj ntsig zoo ib yam rau pawg neeg dag. Cov Vv [glom] thiab N [glom] ntawm pawg kuv tau qis dua cov pawg S (33.7 feem pua thiab 28.3 feem pua), thiab pawg IQ tsis muaj qhov sib txawv tseem ceeb piv rau pawg S.
Cov lus xaus:Quercetin tau ua haujlwm zoo li nephroprotective tus neeg saib xyuas hauv kev tiv thaiv glomerular poob pom thaumraumNws raug rau sov ischemia. Qhov no qhia tias cov flavonoid no yuav siv tau los tiv thaiv hauvraumphais, thaum sov ischemia yog tsim nyog, xws li ib nrab nephrectomy.
Hu rau:joanna.jia@wecistanche.com
cistanche txiv neej cov txiaj ntsig
Taw qhia
Ib nrab nephrectomy tau raug suav hais tias yog tus qauv kub kev kho mob rau lub raum me me hauv zos (1, 2) thiab tau ua tiav ntau ntxiv vim qhov hloov pauv ntawm cov txheej txheem kuaj mob (3). Txawm hais tias muaj kev tshawb fawb tas li thiab kev txhim kho rau hauv cov txheej txheem phais tshiab, uas suav nrog mob khaub thuas ischemia thiab xoom ischemia (4, 5), lub raum sov ischemia tseem feem ntau siv kom tau txais cov ntshav tsis ua haujlwm thaum lub sijhawm nephrectomy (6).
Nyob rau hauv sib piv, lub raum ischemia thaum lub sij hawm nephron-sparing phais yog txuam nrog postoperative glomerular poob thiab ua hauj lwm poob ntawm cov seem parenchyma (7, 8), uas yuav ua rau lub raum tsis ua hauj lwm. Yog li ntawd, cov tshuaj uas yuav muaj kev tiv thaiv los ntawm covraumThaum lub sij hawm ua ntej, perioperative, thiab postoperative lub sij hawm raug lav (9, 10).
Quercetin tau pom cov txiaj ntsig zoo nyob rau hauv cov kev sim sib txawv, feem ntau yog vim nws cov tshuaj tua kab mob antioxidant thiab tiv thaiv kab mob (11-14). Cov flavonoid no kuj tau pom tias muaj kev cia siab thaum muaj kev cuam tshuam nrog sov ischemia hauv cov kabmob thiab cov ntaub so ntswg sib txawv (15-17) thiab, tshwj xeeb tshaj yog, hauvraum(18-20). Txawm li cas los xij, rau qhov zoo tshaj plaws ntawm peb txoj kev paub, quercetin tseem tsis tau tshawb fawb txog kev tiv thaiv glomerular poob tom qab sov ischemia.
Qhov kev xav ntawm txoj kev tshawb fawb tam sim no yog tias quercetin tuaj yeem ua rau lub raum puas tsuaj los ntawm ischemia-reperfusion. Lub hom phiaj yog txhawm rau ntsuas ntau qhov kev tiv thaiv ntev ntev ntawm quercetin thaum lub raum sov ischemia hauv tus qauv nas.
Cov khoom siv thiab cov txheej txheem
Txhua qhov kev sim tau ua tiav raws li kev cai lij choj hauv tebchaws thiab thoob ntiaj teb rau kev siv tsiaj txhu, thiab qhov project no tau raug pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Ncaj Ncees rau Tsiaj Sim (CEUA 040/2017).
Plaub caug tus txiv neej Wistar nas hnub nyoog 9 lub lis piam tau siv. Cov tsiaj tau muab faib ua 4 pawg: Sham (S) - pab pawg xa mus rau laparotomy thiab kev txiav tawm ntawm lub raum pedicle (n=10); Sham Quercetin (SQ) - pab pawg kho nrog quercetin thiab raug rau tib cov txheej txheem raws li pab pawg Sham (n=10); Ischemia (I) - pab pawg xa mus rau lub raum sov ischemia (n=10); Ischemia Quercetin (IQ) - pab pawg kho nrog quercetin thiab xa mus rau lub raum sov ischemia (n=10). Pawg SQ thiab IQ tau txais 50mg / kg quercetin (Quercetin, New Roots Herbal, Vaudreuil- -Dorion, Canada) los ntawm gavage 3 hnub ua ntej thiab 3 hnub tom qab kev phais, thiab ib qho kev tswj hwm intraperitoneal 60 feeb ua ntej kev phais, thaum tsis kho. pab pawg (S thiab kuv) tau txais saline (tsis yog quercetin) siv tib txoj cai.
Txhua tus nas raug tshuaj loog los ntawm intramuscular ketamine (Cetamin, Syntec, Santana de Parnaí-ba, Brazil, 100mg / kg) thiab xylazine (Xilazin, Syntec, 20mg / kg). Raws li cov txheej txheem aseptic, ventral midline incision tau siv los nthuav tawm lub plab viscera, uas tau hloov mus rau sab laug.raum. Sab laug lub raum hlab ntsha thiab cov hlab ntsha raug cais los ntawm blunt dissection. Hauv cov tsiaj ntawm pab pawg kuv thiab IQ, lub raum hlab ntsha raug kaw rau 60 feeb, thaum nyob rau hauv pawg S thiab SQ cov pedicle raug txiav, tab sis tsis muaj ischemia raug ntxias. Tag nrho cov pab pawg tseem siv tshuaj loog rau 60 feeb, thaum lub plab viscera tau hloov mus rau hauv plab, thiab qhov phais tau npog nrog cov ntaub qhwv moistened. Thaum kawg ntawm lub sijhawm no, vascular clamps raug tshem tawm thiab rov ua dua tshiab (hauv pawg kuv thiab IQ), thiab lub plab kab noj hniav raug kaw hauv tus qauv qauv.
Sab lauglub raumtau sau thiab kho hauv 4 feem pua phosphate-buffered formaldehyde. Lub raum tau hnyav, thiab lub raum ntim tau ntsuas siv Scherle txoj kev (21). Qhov cortex-non-cortex cheeb tsam piv (C-NC) tau suav nrog txoj kev Cavalieri (22-24). Lub cortical ntim (CV) tau xam los ntawm kev muab lub raum ntim los ntawm C-NC (24).
Cov tsiaj tau euthanized 4 lub lis piam tom qab kev phais los ntawm kev siv tshuaj loog ntau dhau (Isoflurane, BioChimico, Rio de Janeiro, Brazil). Tam sim ntawd tom qab kev tuag, cov ntshav tau sau los ntawm kev mob plawv. Cov qauv no tau siv rau urea thiab creatinine serum qib tshuaj ntsuam xyuas los ntawm txoj kev automated enzymatic. Sab lauglub raumtau sau thiab kho hauv 4 feem pua phosphate-buffered formaldehyde. Covlub raumraug ntsuas, thiab lub raum ntim tau ntsuas siv Scherle txoj kev (21). Qhov cor-tex-non-cortex cheeb tsam piv (C-NC) tau suav nrog txoj kev Cavalieri (22-24). Lub cortical ntim (CV) tau xam los ntawm kev muab lub raum ntim los ntawm C-NC (24).
Randomly sau cov qauv ntawm txhua sab lauglub raumtau ua tiav rau paraffin embedding, sectioned ntawm 5µm thickness, thiab stained nrog hematoxylin thiab eosin. Kev tshuaj xyuas histological tau ua qhov muag tsis pom kev siv 25 thaj chaw histological, tau los ntawm tsib ntu sib txawv ntawm lub raum cortex los ntawm txhua lub raum. Glomerular volumetric density (Vv [glom]), uas qhia txog qhov sib npaug ntawm qhov ntim ntawm cov glomeruli nyob rau hauv lub cortex, tau kwv yees los ntawm txoj kev suav cov ntsiab lus (22-24). Qhov ntim-hnyav glomerular ntim (VWGV) tau kwv yees siv qhov taw tes-sampled intercepts method (22-24). Kev kwv yees ntawm tag nrho cov glomeruli rau ib lub raum (N[glom]) tau suav nrog cov qauv: CVxVv[glom] / VWGV (24).
Cov txiaj ntsig tau muab piv los ntawm ib txoj kev ANOVA nrog Bonferroni qhov kev xeem posthoc. Kev tshuaj xyuas tau ua tiav siv GraphPad Prism 5.0 (GraphPad Software, San Diego, USA). Tag nrho cov txiaj ntsig tau suav tias yog qhov tseem ceeb thaum p<0.05. all="" numerical="" data="" were="" presented="" as="" mean±standard="">
Tsis tas li ntawd, ib tus kws kho tsiaj uas paub txog cov kab mob pathology (tsis paub txog cov kev kho mob) tau tshuaj xyuas tag nrho cov kab mob histological thiab piav qhia txog cov kev hloov pauv hauv histopathological. Cov kev hloov pauv tau pom tom qab ntawd tau tshaj tawm rau ib pab pawg.
cistanche lub cev tsim
TSEEM CEEB
Cov qib ntshav urea hauv pawg kuv yog 10.4 feem pua siab dua li hauv pawg S. Tsis muaj qhov sib txawv ntawm cov ntshav urea qib tau pom ntawm pawg IQ thiab lwm pab pawg. Tsis tas li ntawd, tsis muaj qhov sib txawv tau pom nyob rau hauv kev tshuaj xyuas ntshav creatinine ntawm txhua pawg.
C-NC ntawm pab pawg kuv tau txo qis los ntawm 9.3 feem pua thaum piv rau pab pawg S. Quercetin kev kho mob hauv pab pawg IQ tau rov qab zoo li qub rau qhov ntsuas no. C-NC ntawm pab pawg IQ yog 5.9 feem pua siab dua ntawm pawg I thiab muaj txiaj ntsig zoo sib xws rau pawg S thiab SQ.
Lub Vv [glom] ntawm pawg kuv yog 32.7 feem pua qis dua li ntawm pawg S thiab 34.2 feem pua raug txo qis piv rau pawg SQ. Lub caij no, pab pawg IQ tau nce 33.7 feem pua hauv kev sib piv rau pawg I, thiab txo qis ntawm 12.0 feem pua hauv kev sib piv rau SQ. Kev kho mob nrog quercetin hauv cov tsiaj uas raug rau lub raum ischemia (pab pawg IQ) tiv thaiv qhov txo qis ntawm Vv [glom] pom hauv cov tsiaj tsis kho.
Cov ischemiclub raum(pab pawg I) nthuav qhia qhov txo N[glom] piv rau pawg S thiab SQ (26.0 feem pua thiab 33.0 feem pua , feem). Kev kho mob nrog quercetin nyob rau hauv pab pawg IQ tiv thaiv qhov glomeruli poob, vim tias pab pawg no tsis muaj qhov sib txawv nrog pawg S thiab SQ, tab sis 28.3 feem pua nce N [glom] piv rau pawg I.
Tsis muaj qhov sib txawv tau sau tseg hauvraumhnyav,raumvolume, cortical volume, thiab VWGV ntawm cov pab pawg. Txhua cov ntaub ntawv tau nthuav tawm hauv Table -1 thiab tau sawv cev hauv daim duab 1 thiab 2.
Kev tshawb fawb histopathological tau qhia txog kev sib cais ntawm cov tubular thiab medullary congestion nyob rau hauv qee cov tsiaj ntawm pawg S thiab SQ, tab sis lub cortical architecture tau khaws cia. Ntawm qhov tod tes, ntau qhov kev tsom xam ntawm kev puas tsuaj parenchymal tau pom nyob rau hauvlub raumntawm pawg I. Qhov no suav nrog cov khoom siv amorphous nyob rau hauv tubular lumen, amorphous eosinophilic khoom nyob rau hauv cheeb tsam medullary, glomerular degeneration, mononuclear infiltration, pom meej poob ntawm glomeruli, thiab poob ntawm cortical architecture. Cov kev tshawb pom pathological no tsawg tsawg hauvlub raumraug rau ischemia raws li kev kho mob quercetin (pab pawg IQ).
Kev sib tham
Txawm hais tias muaj kev siv zog ua kom tsis txhob sov ischemia thaum lub sij hawm ib feem ntawm nephrectomy, nrog rau cov txheej txheem tawm-clamp thiab txias ischemia (4, 5), sov ischemia tseem tsim nyog thaum feem ntau nephron-sparing surgeries (1, 2, 6), thiab nws cov teeb meem thiab mus tas li. rau lub raum muaj nuj nqi thiab cov morphology ntawm cov seem parenchyma (7, 8) yog tshwm sim los ntawm lub siab zus tau tej cov oxygen reactive hom thiab thiaj li lipid peroxidation (25, 26). Muab qhov no, kev siv tshuaj antioxidant raws li qhov ua tau nephron tiv thaiv yog plausible (9-11, 18-20) nrog rau lwm yam kabmob thiab cov ntaub so ntswg (12-17) cuam tshuam nrog ischemia / reperfusion (9, 10, 15-20, 26).
Quercetin yog ib qho flavonoid uas tau ua pov thawj tias muaj txiaj ntsig zoo hauv kev tawm tsam kev tsim cov pa oxygen reactive thiab tau hloov kho cov txiaj ntsig zoo ntawm biochemical cim ntawm homeostatic zog ntawm lub cev thiab cov ntaub so ntswg (12-17), suav nroglub raumnyob rau hauv ntau qhov xwm txheej (11) xws li sov ischemia (18-20). Peb cov txiaj ntsig ntau tau lees paub tias kev siv quercetin muaj txiaj ntsig zoo hauv lub raum ischemia. Nws tau xav tias cov txiaj ntsig kev tiv thaiv tau pom nyob rau hauv cov tsiaj uas tau txais quercetin yog qhov tshwm sim ntawm kev txo qis oxidative puas hauv lub raum cov ntaub so ntswg. Txawm li cas los xij, oxidative kev nyuaj siab tsis tau raug soj ntsuam ncaj qha hauv txoj kev tshawb fawb tam sim no, ib yam li lwm tus (27), thiab cov kev tshawb fawb yav tom ntej yuav tsum qhia meej txog cov txheej txheem ntawm kev ua. Kev siv cov flavonoid no tau zoo los tiv thaiv glomerular poob (N [glom]), uas tshwm sim tsuas yog hauv cov tsiaj uas raug rau lub raum ischemia yam tsis muaj kev kho mob quercetin.
Txawm hais tias qhov tseem ceeb ntawm txoj kev tshawb fawb tam sim no yog kev ntsuam xyuas histo-morphometric, cov ntshav urea thiab creatinine qib kuj tau txheeb xyuas, uas yog cov cim siv biochemical feem ntau ntawm lub raum ua haujlwm (28). Serum urea nce tsuas yog nyob rau hauv pawg ischemia, ntxiv dag zog rau kev kho mob nrog quercetin tiv thaiv lub raum tsis ua haujlwm raws li cov cim qhia no. Qhov no yog raws li cov ntaub ntawv yav dhau los qhia hauv kev tshawb fawb yav dhau los (18-20).
Nephron sparing phais muaj, raws li lub npe qhia, lub hom phiaj ntawm kev tshem tawm me meraumpawg thaum khaws cia ntau tshaj plaws ntawm nephrons los khaws lub raum ua haujlwm nyob rau lub sijhawm ntev (1, 2, 6-8). Yog li ntawd, kom muaj nuj nqis ntawm tus naj npawb ntawm glomeruli, uas yog qhov tshwm sim uas kwv yees tus naj npawb ntawm nephrons tam sim no nyob rau hauv parenchyma (10, 22-24), muab cov txiaj ntsig tseeb uas qhia tau tias yog ib feem ntawm nephrectomy mus txog nws lub hom phiaj. Rau qhov zoo tshaj plaws ntawm peb txoj kev paub, txoj kev tshawb no yog thawj zaug qhia txog kev tiv thaiv cov teebmeem ntawm quercetin ntawm glomerular poob los ntawm lub raum sov ischemia.
Cov qauv nas yog siv dav hauv cov kev tshawb fawb tsom rau qhov cuam tshuam ntawm lub raum ischemia / reperfusion (9, 18-20, 22), thiab cov kev hloov morphometric tshwm sim los ntawm cov txheej txheem no twb tau ua pov thawj (22). Peb cov txiaj ntsig tau zoo ib yam nrog cov ntawv tshaj tawm dhau los ntawm 60-feeb sov lub raum ischemia hauv tus qauv no (22), nrog kev txo qis hauv C-NC, Vv[glom], thiab N[glom] hauv pawg ischemia piv rau pawg sham.
Sham tsiaj kho nrog quercetin qhia tsis muaj kev kho mob, biochemical, los yog morphometric quantitative lub raum hloov, uas qhia tau hais tias quercetin tsis muaj kev phiv los ntawm lub raum.raum, uas yog raws li kev tshawb fawb yav dhau los (18-20, 26, 29). Qhov tseem ceeb tshaj, peb cov txiaj ntsig tau lees paub tias qhov flavonoid no tiv thaiv glomerular poob los ntawm ischemia / reperfusion txij li qhov txiaj ntsig ntawm Vv [glom] thiab N [glom] ntawm pawg Ischemia kho nrog quercetin zoo ib yam li cov pab pawg neeg txaj muag.
Cov kev tshawb fawb luam tawm yav dhau los tau qhia txog kev tiv thaiv ntawm quercetin nyob rau lub sijhawm luv luv, raws li kev tshuaj xyuas ntawm cov kev tshawb fawb no tau ua ib hnub tom qab lub raum sov ischemia (18-20). Txoj kev tshawb no ntxiv cov ntaub ntawv ntawm lub ncauj lus no, tsom xam covlub raum4 lub lis piam tom qab kev raug mob ischemic, yog li qhia tau hais tias qhov kev tiv thaiv ntawm quercetin yog ruaj khov.
Txawm li cas los xij, nws yuav tsum raug sau tseg tias qhov kev tshawb fawb tam sim no muaj qee qhov kev txwv. Lub pathophysiological teb rau ischemic raug mob txawv ntawm hom. Txawm hais tias tus qauv nas ntawm lub raum sov ischemia tau siv dav, qhov no tseem yog qhov chaw sim, nrog ntau qhov sib txawv rau qhov chaw kho mob. Tsis tas li ntawd, kev ntsuam xyuas ntawm lub raum tshem tawm thiab lwm yam raum cov ntshav biomarkers yuav xav tau rau kev soj ntsuam kev ua haujlwm zoo dua. Cov kev tshawb fawb soj ntsuam yav tom ntej tshawb xyuas kev siv quercetin ua cov tshuaj nephroprotective hauv cov neeg mob uas tau txais ib feem ntawm nephrectomy nrog sov ischemia yog lav.
Yog li, nws tau txiav txim siab tias quercetin tau txais txiaj ntsig zoo li nephroprotective tus neeg sawv cev, txo cov kev hloov kho histopathological, tiv thaiv glomerular poob, thiab tswj hwm cov qib urea hauv cov ntshav tom qab sov ischemia. Cov txiaj ntsig no qhia tau tias cov flavonoid no tuaj yeem txhim kho lub raum ua haujlwm ntev - -lub raum ua haujlwm hauv cov neeg mob uas tau txais ib feem ntawm nephrectomy nrog sov ischemia.
Cistanche tubulosa tiv thaiv kab mob raum, nyem qhov no kom tau txais cov qauv
NYIAJ
Kev sib koom ua ke rau kev txhim kho tom qab-
-Cov Tub Ntxhais Kawm Tiav - CAPES
Lub Foundation rau Kev Tshawb Fawb Txhawb ntawm Rio de
Janeiro - FAPERJ
National Council for Scientific and Technological Development - CNPq
Kev tsis sib haum xeeb OF NYIAJ
Tsis muaj leej twg tshaj tawm.
REFERENCES
1. Campbell S, Uzzo RG, Allaf ME, Bass EB, Cadeddu JA, Chang A, et al. Renal Mass and Localized Renal Cancer: AUA Guideline. J Urol. 2017; 198:{3}} ib.
2. Cai Y., Li Z., Zhang YS. Kev sib piv ntawm Ib feem thiab Radical Laparoscopic Nephrectomy: Cov txiaj ntsig ntev rau kev kho mob T1b raum Cell Carcinoma. Urol J. 2018; 15:{5}}.
3. Salameh JP, McInnes MDF, McGrath TA, Salameh G, Schieda N. Diagnostic Accuracy of Dual-Energy CT for Evaluation of Renal Masses: Systematic Review and Meta-Analysis. AJR Am J Roentgenol. 2019; 212: W 105.
4. Simone G, Capitanio U, Tuderti G, Presicce F, Leonardo C, Ferriero M, thiab al. On-clamp piv rau off-clamp ib nrab nephrectomy: Propensity qhab-nees sib piv ntawm cov txiaj ntsig ua haujlwm ntev. Int J Urol. 2019; 26:7 :ua.
5. Bertolo R, Garisto J, Dagenais J, Agudelo J, Armanyous S, Lioudis M, et al. Txias Versus Sov Ischemia Robot-Assisted Partial Nephrectomy: Sib piv ntawm Kev Ua Haujlwm Zoo Tshaj Plaws hauv Propensity-Score Matched "Tshem Tawm" Cov Neeg Mob. J Endourol. 2018; 32:{5}}.
6. Hur M, Park SK, Shin J, Choi JY, Yoo S, Kim WH, et al. Qhov cuam tshuam ntawm cov chaw taws teeb ischemic preconditioning ntawm cov ntshav creatinine hauv cov neeg mob uas tau txais ib feem ntawm nephrectomy: kev kawm raws tu qauv rau kev sim tshuaj randomized. Kev sim. 2018; 19:473 ib.
7. Lee H, Song BD, Byun SS, Lee SE, Hong SK. Kev cuam tshuam ntawm lub sijhawm sov ischemia ntawm lub raum ua haujlwm tom qab lub raum ua haujlwm tom qab ib nrab nephrectomy rau kev kho mob T1 lub raum cell carcinoma: txoj kev tshawb fawb txog kev sib tw. BJU Int. 2018; 121:{5}} ib.
8. Zabell JR, Wu J, Suk-Ouichai C, Campbell SC. Lub raum ischemia thiab cov txiaj ntsig ua haujlwm tom qab ib nrab nephrectomy. Urol Clin North Am. 2017; 44:{4}}.
9. Ozkan TA, Karakoyunlu N, Polat R, Sarıbaş GS, Şener NC, Özdemir S, et al. Kev ntsuam xyuas ntawm kev tiv thaiv cov nyhuv ntawm esomeprazole hauv kev sim qauv ntawm lub raum ischemia-reperfusion. Urol Nephrol Cov. 2018; 50:{4}.
10. Damasceno-Ferreira JA, Abreu LAS, Bechara GR, Costa WS, Pereira-Sampaio MA, Sampaio FJB, et al. Mannitol txo qis nephron poob tom qab sov lub raum ischemia hauv cov qauv porcine. BMC Urol. 2018; 18:16.
11. Abharzanjani F, Afshar M, Hemmati M, Moussavi M. Short-term High Dose of Quercetin thiab Resveratrol Alters Aging Markers in HumanLub raumCells. Int J Prev Med. 2017; 8:64 ib.
12. Patra A, Satpathy S, Shenoy AK, Bush JA, Kazi M, Hussain
MD. Kev tsim thiab kev ntsuam xyuas ntawm cov khoom sib xyaw polymeric micelles ntawm quercetin rau kev kho mob ntawm lub mis, zes qe menyuam, thiab ntau yam tshuaj tiv thaiv kab mob. Int J Nanomedicine. Xyoo 2018; 13:2869-81.
13. Wang D, Lou X, Jiang XM, Yang C, Liu XL, Zhang N. Quercetin tiv thaiv o, MMP-2 ua kom, thiab apoptosis induction nyob rau hauv tus qauv nas ntawm cardiopulmonary resuscitation los ntawm modulating Bmi{{2} } lus. Mol Med Rep. 2018; 18:{5}}.
14. Srinivasan P, Vijayakumar S, Kothandaraman S, Palani M. Anti-diabetic kev ua ntawm quercetin muab rho tawm los ntawm Phyllanthus Emblica L. txiv hmab txiv ntoo: Hauv silico thiab hauv vivo mus kom ze. J Pharm Anal. Xyoo 2018; 8:109-18.
15. Tóth Š, Jonecová Z, Čurgali K, Maretta M, Šoltés J, Švaňa M, et al. Quercetin attenuates cov ischemia reperfusion-induced COX-2 thiab MPO qhia nyob rau hauv cov hnyuv mucosa. Biomed Pharmacother. 2017; 95:5 :ua.
16. Park DJ, Shah FA, Koh PO. Quercetin attenuates neuronal hlwb puas nyob rau hauv nruab nrab cerebral hlab ntsha occlusion tsiaj qauv. J Vet Med Sci. 2018; 80: 3:ua.
17. Uyla MU, ahin A, ahintürk V, Alata Ö. Quercetin cuam tshuam rau txoj hmoo ntawm hepatic ischemia thiab reperfusion raug mob hauv nas: Kev tshawb fawb sim. Int J Surg. 2018; 53: 3.
18. Kahraman A, Erkasap N, Serteser M, Köken T. Tiv thaiv cov nyhuv ntawm quercetin ntawm lub raum ischemia/reperfusion raug mob hauv nas. J Nephrol. 2003; 16: 219-24.
19. Kinaci MK, Erkasap N, Kucuk A, Koken T, Tosun M. Cov teebmeem ntawm quercetin ntawm apoptosis, NF-B thiab NOS gene qhia nyob rau hauv lub raum ischemia/reperfusion raug mob. Exp Ther Med. 2012; 3:{4}}.
20. Chen BL, Wang LT, Huang KH, Wang CC, Chang CK, Liu SH. Quercetin attenuates lub raum ischemia / reperfusion raug mob los ntawm kev ua kom lub AMP-activated protein kinase-regulated autophagy txoj kev. J Nutr Biochem. 2014; 25: 1226-34
