Kev Ntsuas Zoo Thiab Kev Ntsuas Kev Ntsuas Ntawm Cistanche Deserticola Los Ntawm Cov Keeb Kwm Hauv Suav Teb Ⅱ

2 Cov txiaj ntsig thiab kev sib tham

2.1 Cov ntsiab lus ntawm bioactive tshuaj nyob rau hauv Cistanche deserticola los ntawm txawv keeb kwm

Daim duab 2 qhia tag nrho cov ntsiab lus polyphenol ntawmCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 2 tias, tshwj tsis yog lub hauv paus chiv keeb A, tag nrho cov ntsiab lus polyphenol ntawm cov hauv paus hniav ntawm lwm tsib cov qauv hauv paus chiv keeb tau ntau dua qhov nruab nrab thiab sab saum toj. Ntawm lawv, tag nrho cov ntsiab lus polyphenol ntawm cov qauv los ntawm keeb kwm D yog qhov siab tshaj plaws, thiab cov ntsiab lus ntawm cov hauv paus, nruab nrab thiab sab saum toj yog 20.06, 17.33 thiab 15.67 mg GAE / g DW, feem. Ua raws li keeb kwm B (raws li 13.85, 12.93

Fig.2 Tag nrho cov ntsiab lus polyphenol ntawm CD los ntawm txawv keeb kwm

Fig.3 Tag nrho cov ntsiab lus flavonoid ntawm CD los ntawm txawv keeb kwm

thiab 7.58 mg GAE/g DW), keeb kwm C (14.64, 6.48 thiab 13.48 mg GAE/g DW, feem). Tag nrho cov ntsiab lus polyphenol ntawm cov qauv los ntawm keeb kwm E thiab keeb kwm F yog qis dua, thiab cov ntsiab lus ntawm txhua feem yog qis dua 5.00 mg GAE/g DW.

Tau txais Cov Khoom Siv Zoo Cistanche

Daim duab 3 qhia tag nrho cov ntsiab lus flavonoid ntawmCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 3 tias tag nrho cov ntsiab lus flavonoid nyob rau sab saum toj yog siab dua li qhov nruab nrab thiab hauv qab hauv cov qauv los ntawm lwm tsib lub hauv paus ntsiab lus tshwj tsis yog F. Tag nrho cov ntsiab lus flavonoid ntawm cov qauv ntawm cov chaw tsim khoom E thiab F yog qhov tseem ceeb. siab dua li ntawm lwm qhov chaw tsim khoom, thiab tag nrho cov ntsiab lus flavonoid ntawm E3 qauv yog qhov siab tshaj (21.96 mg RE / g DW); tag nrho cov ntsiab lus flavonoid ntawm cov qauv C1 yog qhov qis tshaj, tsuas yog 0.54 mg RE/g DW.

Daim duab 4 qhia tag nrho cov ntsiab lus triterpenoid ntawmCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 4 tias tag nrho cov ntsiab lus triterpenoid hauv cov qauv los ntawm rau lub hauv paus yog nyob rau hauv qhov kev txiav txim ntawm hauv paus> nruab nrab> saum. Cov qauv los ntawm keeb kwm A muaj cov ntsiab lus siab tshaj plaws ntawm tag nrho cov triterpenes, cov ntsiab lus hauv paus yog siab li 19.88 mg OAE / gDW, thiab nruab nrab thiab sab saum toj yog 19.01 thiab 16.99 mg OAE / g DW, feem. Ua raws li keeb kwm F, nws cov ntsiab lus hauv paus kuj mus txog 19.62 mg OAE / g DW, thiab cov ntsiab lus nruab nrab thiab sab saum toj yog 17.15 thiab 15.17 mg OAE / g DW, feem; tag nrho cov ntsiab lus triterpenoid ntawm cov qauv ntawm A thiab F yog qhov siab dua li ntawm plaub lub hauv paus chiv keeb.

Fig.4 Tag nrho cov ntsiab lus triterpene ntawm CD los ntawm txawv keeb kwm

Daim duab 5 qhia tag nrho cov ntsiab lus polysaccharide ntawmCistanche deserticolafrom different origins. It can be seen from Figure 5 that in the samples from the six origins, the polysaccharide content in the root is higher than that in the middle and top, and the order is root>middle>saum. Tag nrho cov ntsiab lus polysaccharide ntawm cov qauv los ntawm keeb kwm F yog qhov siab tshaj plaws, thiab cov ntsiab lus ntawm cov hauv paus hniav, nruab nrab thiab sab saum toj yog 35.44, 32.76 thiab 25.34 mg DE / g DW, feem. Qhov no tau ua raws li keeb kwm E, uas muaj cov ntsiab lus siab dua ntawm 35.92 mg DE / g DW hauv paus, 29.82 thiab 26.26 mg DE / g DW hauv nruab nrab thiab sab saum toj. Tag nrho cov ntsiab lus polysaccharide ntawm cov qauv los ntawm ob lub hauv paus chiv keeb E thiab F tau ntau dua li ntawm plaub lub hauv paus chiv keeb; tag nrho cov ntsiab lus polysaccharide ntawm cov qauv los ntawm keeb kwm D yog qhov qis tshaj, thiab nws cov hauv paus, nruab nrab thiab cov ntsiab lus saum toj kawg nkaus tsuas yog 10.60, 9.92 thiab 9.00 mg DE / g DW.

Fig.5 Tag nrho cov ntsiab lus polysaccharide ntawm CD los ntawm txawv keeb kwm

Daim duab 6 qhia tag nrho cov ntsiab lus proanthocyanidin ntawmCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 6 tias cov ntsiab lus ntawm tag nrho cov procyanidins nyob rau hauv nruab nrab ntawm cov qauv los ntawm keeb kwm A, C thiab E yog siab dua li ntawm cov hauv paus hniav thiab sab saum toj, uas yog 4.58, 3.60 thiab 3.03 mg PCE / g DW, feem. . Cov ntsiab lus ntawm tag nrho cov procyanidins nyob rau hauv lub hauv paus ntawm cov qauv los ntawm keeb kwm B yog siab tshaj qhov nruab nrab thiab sab saum toj, uas yog 4.87 mg PCE / g DW; cov ntsiab lus ntawm tag nrho cov procyanidins nyob rau sab saum toj ntawm cov qauv los ntawm keeb kwm D thiab F yog siab dua li cov hauv paus hniav thiab nruab nrab, uas yog 4.91 thiab 6.14 mg PCE / g DW, feem. Feem ntau, qhov nruab nrab cov ntsiab lus ntawm tag nrho cov procyanidins nyob rau hauv peb qhov chaw ntawm ntau lawm F yog qhov siab tshaj plaws, uas yog 4.71 mg PCE / g DW, thiab qhov nruab nrab cov ntsiab lus ntawm tag nrho cov procyanidins nyob rau hauv peb qhov chaw ntawm ntau lawm cheeb tsam E yog qhov qis tshaj, tsuas yog 2.25 mg PCE/g DW.

Daim duab 7 qhia cov ntsiab lus ntawmechinacoside cov tshuajhauvCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 7 tias cov ntsiab lus ntawm echinacea nyob rau hauv cov hauv paus hniav ntawm cov qauv los ntawm lwm lub hauv paus chiv keeb yog ho siab tshaj qhov nruab nrab thiab sab saum toj qhov chaw tshwj tsis yog rau keeb kwm A. Cov qauv ntawm keeb kwm C muaj cov ntsiab lus echinacoside siab tshaj plaws, nrog rau cov hauv paus hniav. , nruab nrab thiab sab saum toj cov ntsiab lus ntawm 25.11, 12.5 thiab 15.65 mg / g DW, feem. Keeb kwm F cov qauv muaj cov ntsiab lus qis tshaj echinacoside,

Lub hauv paus, nruab nrab thiab cov ntsiab lus saum toj kawg nkaus yog 2.37, 1.09 thiab 0.68 mg / g DW, feem.

Daim duab 8 qhia txog cov ntsiab lus ntawm glucosides hauvCistanche deserticolalos ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 8 tias cov hauv paus hniav ntawm cov qauv los ntawm keeb kwm B, C, E thiab F muaj ntau dua verbascoside cov ntsiab lus, uas yog 4.78, 3.80, 0.70 thiab 0.33 mg/g DW, feem. Cov ntsiab lus saum toj kawg nkaus ntawm cov qauv los ntawm keeb kwm A yog qhov siab tshaj plaws, ntawm 9.74 mg / g DW; Cov ntsiab lus nyob hauv nruab nrab ntawm cov qauv los ntawm keeb kwm D yog qhov siab tshaj plaws, ntawm 8.30 mg / g DW. Ntawm lawv, cov ntsiab lus ntawm verbascoside hauv cov qauv los ntawm thaj chaw tsim khoom D yog qhov siab dua li ntawm lwm qhov chaw tsim khoom, nrog rau cov ntsiab lus nruab nrab ntawm 6.98 mg / g DW. Cov qauv los ntawm keeb kwm E thiab F tau qis dua ntawm verbascoside, nrog rau qhov nruab nrab ntawm 0.46 thiab 0.28 mg / g DW, feem.

Fig.7 Cov ntsiab lus echinacoside ntawm CD los ntawm txawv keeb kwm

Fig.8 Cov ntsiab lus verbascoside ntawm CD los ntawm txawv keeb kwm

Li Biao [26] muab faib cov Cistanche sau rau hauv sab sauv, nruab nrab thiab qis, thiab ntsuas cov ntsiab lus ntawm echinacoside thiab verbasin. Nws tau pom tias cov ntsiab lus ntawm qhov sib txawv yog qhov sib txawv. siab tshaj qhov nruab nrab thiab sab sauv. Cov ntsiab lus ntawm tag nrho cov polyphenols, tag nrho triterpenes, tag nrho polysaccharides, verbascoside thiab echinacoside nyob rau hauv cov hauv paus hniav ntawm feem ntau cov qauv yog kuj siab nyob rau hauv cov qauv ntawmCistanche deserticolakuaj hauv daim ntawv no. Ji Xiaohui [36] thiab lwm tus tau kawm txog bioactive Cheebtsam ntawmCistanche deserticolalos ntawm Gurbantunggut Desert hauv Xinjiang, thiab pom tias nyob rau hauv cov qauv ntawm qhov sib txawv, cov ntsiab lus ntawm active Cheebtsam nyob rau hauv lub cev nqaij daim tawv qia (qis) yog siab dua, thiab cov ntsiab lus nyob rau hauv nruab nrab thiab sab saum toj yog qis dua. Qhov tshwm sim no tuaj yeem cuam tshuam nrog kev loj hlob ntawm cov menyuam yaus thiab kev thauj mus los ntawm cov khoom nojCistanche deserticola. Nyob rau tib lub sijhawm, Ji Xiaohui thiab lwm tus pom tias cov ntsiab lus ntawm cov khoom nquag ntawm Cistanche deserticola ua nyob rau hauv ntau thaj tsam ntawm tib lub suab puam kuj txawv ntawm qhov sib txawv. Liu Xiong[37] thiab lwm tus pom tias cov ntsiab lus ntawm echinacoside hauv Cistanche deserticola los ntawm cov hauv paus chiv keeb txawv txawv heev (0.42 feem pua ​​-0.52 feem pua ​​).

Zhao Zhihong [38] thiab lwm tus tau hais tias qhov sib txawv ntawm qhov chaw nyob hauv huab cua, av yam, thiab hydrothermal tej yam kev mob ntawmCistanche deserticolanyob rau hauv ntau qhov chaw tsim khoom yuav ua rau cov yam ntxwv sib txawv thiab cov qauv tsis zoo, uas yuav cuam tshuam rau kev kho mob.

2.2 Antioxidant kev ua ntawm Cistanche deserticola los ntawm txawv keeb kwm

Cov qauv cog muaj ntau yam bioactive Cheebtsam nyob rau tib lub sijhawm, thiab cov khoom sib txawv tuaj yeem ua rau muaj kev sib koom ua ke lossis kev tawm tsam, yog li kev txiav txim siab ntawm antioxidant peev xwm ntawm cov qauv rho tawm yog tus sawv cev ntau dua li cov kev ua haujlwm antioxidant ntawm ib feem [39].

Daim duab 9 qhia IC50 qhov tseem ceeb ntawm DPPH dawb radicals hauv Cistanche deserticola los ntawm ntau lub hauv paus chiv keeb. Nws tuaj yeem pom los ntawm daim duab 9 tias IC50 qhov tseem ceeb ntawm qhov sib txawv ntawm cov qauv los ntawm keeb kwm D yog qhov tsawg tshaj plaws (0.37, 0.46 thiab 0.32 mg / mL, raws li), qhia tias qhov no cov qauv muaj qhov muaj peev xwm ua kom muaj zog tshaj plaws rau DPPH dawb radicals. Ua raws li cov qauv los ntawm keeb kwm B thiab C, uas tseem muaj peev xwm tshem tawm DPPH dawb radicals, IC50 qhov tseem ceeb ntawm lub hauv paus, nruab nrab thiab sab saum toj yog 4.00, 1.74, 3.14 mg / mL thiab 1.56, 4.31, 2.65 mg / mL, feem. . Lub peev xwm scavenging ntawm keeb kwm F kuaj tawm tsam DPPH radicals

Qhov tsis muaj zog tshaj plaws, IC50 qhov tseem ceeb ntawm nws qhov sib txawv tau nce siab dua li ntawm tsib lub hauv paus chiv keeb (P<>

Daim duab 10 qhia txog IC50 qhov tseem ceeb ntawm ABTS dawb radical scavenging tus nqi ntawm Cistanche deserticola los ntawm txawv keeb kwm. Nws tuaj yeem pom los ntawm daim duab 10 tias cov qauv los ntawm keeb kwm B, C thiab D muaj qhov tsawg tshaj plaws IC50 qhov tseem ceeb, thiab qhov nruab nrab IC50 qhov tseem ceeb ntawm peb ntu yog 1.91, 1.89 thiab 1.48 mg / mL, raws li, qhia tias cov qauv ntawm cov no. peb lub hauv paus pib muaj kev cuam tshuam rau ABTS dawb radicals. Muaj peev xwm meej. Cov qauv ntawm lub hauv paus chiv keeb F muaj qhov tsis muaj peev xwm tshem tawm qhov tsis muaj zog tshaj plaws rau ABTS dawb radicals, thiab IC50 qhov tseem ceeb ntawm cov hauv paus hniav, nruab nrab thiab sab saum toj tau nce siab dua li ntawm lwm tsib lub hauv paus chiv keeb (5.98, 6.7 thiab 8.05 mg / mL, feem).

Daim duab 11 qhia txog IC50 qhov tseem ceeb ntawm hydroxyl radical scavenging tus nqi ntawm Cistanche deserticola los ntawm ntau lub hauv paus chiv keeb. Nws tuaj yeem pom los ntawm daim duab 11 tias keeb kwm C, D

Cov qauv ntawm thiab E muaj zog scavenging peev xwm rau hydroxyl radicals, thiab qhov nruab nrab IC50 qhov tseem ceeb ntawm peb qhov chaw yog 3.05, 5.91 thiab 3.05, feem.

5.90 mg/ml Nws tau txiav txim siab tias lub peev xwm khawb ntawm cov qauv A rau hydroxyl radicals tsis muaj zog, thiab IC50 qhov tseem ceeb ntawm nws cov hauv paus, nruab nrab thiab sab saum toj yog feem.

51.01, 41.06 thiab 40.62 mg / mL.

Txhawm rau ua kom tiav, tshwj tsis yog cov qauv los ntawm keeb kwm F, cov qauv los ntawm lwm lub hauv paus chiv keeb pom muaj zog antioxidant muaj peev xwm. Bao Bin [39] and He Meng

Meng[8] thiab lwm tus tau kawm txog kev tshem tawm lub peev xwm ntawm methanol, ethanol thiab dej rho tawm ntawm Cistanche deserticola rau ABTS thiab DPPH dawb radicals, thiab tau txais cov txiaj ntsig.

tuaj rau cov lus xaus zoo sib xws.

Table 6 qhia qhov nruab nrab tus nqi ntawm txhua qhov ntsuas ntsuas hauv cov pawg sib txawv.

Nws tuaj yeem pom los ntawm cov lus hais tias qhov nruab nrab cov ntsiab lus ntawm echinacoside hauv pawg 1 yog qhov siab tshaj plaws, uas yog 12.59 mg / g DW; Qhov nruab nrab cov ntsiab lus ntawm tag nrho cov polyphenols, procyanidins thiab verbascoside nyob rau hauv pawg 2 yog siab dua, uas yog 17.67 mgGAE / g DW thiab 4.51 mg PCE / g feem. DW thiab 6.98 mg / g DW; cov ntsiab lus ntawm tag nrho cov flavonoids, tag nrho triterpenes thiab tag nrho polysaccharides tau qis dua, 6.52 mg RE / g DW, 11.90 mg OAE / g DW, 9.84 mg DE / g DW, feem; Cov ntsiab lus nruab nrab ntawm tag nrho cov triterpenes thiab tag nrho cov polysaccharides yog qhov siab tshaj plaws, uas yog 13.64 mg RE / g DW, 14.79 mg OAE / g DW thiab 30.93 mg DE / g DW, raws li, thiab cov ntsiab lus nruab nrab ntawm proanthocyanidins, echinacoside thiab verbascoside yog cov qis tshaj.

Pawg 2 muaj peev xwm tshem tawm tau zoo rau OH, DPPH thiab ABTS dawb radicals, thiab nws qhov nruab nrab IC50 qhov tseem ceeb yog 5.91, 0.38, thiab 1.48 mg / mL, feem; pab pawg 3 muaj qhov tsis muaj zog scavenging rau DPPH thiab ABTS dawb radicals, nrog qhov nruab nrab IC50 qhov tseem ceeb ntawm 5.91, 0.38, thiab 1.48 mg / mL feem. 81.11, 5.19 mg/ml. Los ntawm kev txheeb xyuas pawg ntawm Cistanche deserticola los ntawm ntau lub hauv paus chiv keeb, nws tau pom tias cov ntsiab lus ntawm echinacoside thiab verbascoside hauv pawg 1 thiab 2 tau ntau dua li cov hauv pawg 3.

Plaub lub hauv paus chiv keeb ntawm pawg 1 thiab 2 tau muab faib rau hauv Inner Mongolia thiab Gansu, thiab pawg 3 raug faib rau sab qaum teb Xinjiang. Cov txiaj ntsig no yog qhov tseem ceeb raws li kev tshawb fawb ntawm Ji Xiaohui li al. [36] ntawm suab puam Cistanche. Nws muaj txiaj ntsig zoo rau kev sib sau ntawm verbascoside thiab echinacoside hauv Cistanche deserticola. Nyob rau tib lub sijhawm, nws tau pom tias cov ntsiab lus polysaccharide ntawm cov suab puam Cistanche suab puam nyob rau sab qaum teb Xinjiang tau nce siab dua li hauv Inner Mongolia thiab Gansu cheeb tsam.

Kev sib sau ntawm cov suab thaj sab hauv [44-45], cov kev cuam tshuam tshwj xeeb xav tau kev kawm ntxiv. Nyob rau tib lub sijhawm, los ntawm txoj kev tshawb no, nws tau pom tias Cistanche deserticola muaj ntau yam tshuaj lom neeg lom neeg. Yog li ntawd, nyob rau hauv kev tshawb fawb txog qhov zoo ntawm Cistanche deserticola, ntxiv rau lub ntsiab ntsuas echinacoside thiab verbascoside teev nyob rau hauv Suav Pharmacopoeia, lwm yam active cov khoom xyaw yuav tsum tau ua ke rau Comprehensive kev ntsuam xyuas.

Cistanche ntsiav tshuaj

3 Kev xaus

Hauv daim ntawv no, cov tshuaj bioactive thiab cov haujlwm antioxidant ntawmCistanche deserticolalos ntawm txawv keeb kwm nyob rau hauv kuv lub teb chaws raug muab piv thiab soj ntsuam. Cov txiaj ntsig tau pom tias thaj chaw tsim khoom thiab qhov chaw kuaj pom muaj qhov cuam tshuam loj rau cov ntsiab lus ntawm bioactive tshuaj nyob rau hauvCistanche deserticola, thiab cov ntsiab lus ntawm bioactive tshuaj nyob rau hauv cov hauv paus hniav ntawm feem ntau cov qauv yog ho siab tshaj qhov nruab nrab thiab sab saum toj. Peb lub ntsiab lus tseem ceeb tau muab rho tawm los ntawm kev txheeb xyuas cov ntsiab lus tseem ceeb, thiab cov txiaj ntsig sib txawv ntawm qhov sib txawv yog 85.43 feem pua. Lub hauv paus chiv keeb nrog cov kev ua tau zoo tshaj plaws yog D, tom qab ntawd los ntawm C thiab A. Kev tshuaj xyuas pawg tau faib cov qauv ua peb pawg, ntawm cov pab pawg thib ib (A, B, C) thiab pawg thib ob (D) tau muab faib rau hauv Inner Mongolia thiab Gansu cheeb tsam, thiab cov qhab nia zoo suav nrog plaub sab saum toj, thiab tag nrho qhov zoo yog qhov zoo. Pab pawg thib peb (E, F) pib hauv Xinjiang, thiab tag nrho cov qhab nia qis. Cov txiaj ntsig kev tshawb fawb yuav muab cov ntaub ntawv tseem ceeb rau kev ntsuas kev ua tau zoo, kev txhim kho cov khoom lag luam thiab kev tsim qauv ntawm Cistanche deserticola los ntawm ntau lub hauv paus hauv kuv lub teb chaws.