Cog Cell Culture As Emerging Technology For Production Of Active Cosmetic Ingredients

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

Vasil Georgiev 1,2 Anton Slavov 2 Ivelina Vasileva 2 Atanas Pavlov 1,3

Nroj tsuagib txwm yog lub hauv paus tseem ceeb ntawm cov khoom xyaw kom zoo nkauj, muaj pov thawj kev noj qab haus huv zoo rau tib neeg, xws li tiv thaiv kev laus, antioxidant, tiv thaiv kev mob, UV-tiv thaiv, tiv thaiv qog noj ntshav, tiv thaiv wrinkle, tawv nqaij soothing, whitening, moisturizing, thiab lwm yam. Extracts los ntawm tshuaj ntsuab, aromatic, thiab/los yog tshuajnroj tsuagtau dav siv los ua cov khoom xyaw nquag hauvcosmeceuticalslos yog nutricosmetics, tshwj xeeb tshaj yog nyob rau hauv cov khoom siv tshuaj pleev ib ce thiab kev tu tawv nqaij formulations. Txawm li cas los xij, dhau kaum xyoo dhau los, tau muaj kev txaus siab ntxiv rau cog cov kab lis kev cai ntawm tes-derivedactive cov khoom xyaw kom zoo nkauj. Cov no yog "tiam tshiab" ntawm cov khoom lag luam zoo, tsim los ntawm cov txheej txheem niaj hnub biotechnology, uas feem ntau pom muaj zog tshaj li cov nroj tsuag rho tawm tau los ntawm cov txheej txheem classical. Hauv qhov kev tshuaj xyuas no, qhov zoo thiab qhov kev vam meej tam sim no hauv cov nroj tsuag kab lis kev cai thev naus laus zis rau kev tsim cov khoom xyaw kom zoo nkauj tau raug sau tseg thiab sib tham txog cov ncauj lus kom ntxaws hauv cov ntaub ntawv nthuav tawm rau kev txhim kho calendula qia cell.

Ntsiab lus:Calendula officinalis L.Cosmeceuticals/ Nutricosmetics / Plant cells / Cog stemcells /Polysaccharides/ Cov ntaub so ntswg thiab kab lis kev cai

cistanche-natural nourishing nroj tsuag

1 Kev Taw Qhia

Nroj tsuagyog qhov qub tshaj plaws ntawm cov khoom sib txuas nrog cov tshuaj thiab tshuaj pleev ib ce uas tau tshawb pom los ntawm noob neej. Txawm tias tam sim no, nruab nrab ntawm 70 thiab 80 feem pua ​​​​ntawm cov neeg thoob ntiaj teb tso siab rau cov tshuaj ntsuab tshuaj ntsuab los npog lawv cov kev xav tau kev noj qab haus huv yooj yim [1].Qhov tseeb, 11 feem pua ​​​​ntawm cov tshuaj tseem ceeb uas muaj nyob rau hauv cov tshuaj niaj hnub tib neeg tseem yog cov nroj tsuag keeb kwm [2]. Qhov feem pua ​​​​ntawm cov khoom siv los ntawm cov khoom siv ntuj tsim, siv los ua cov khoom xyaw nquag hauv tshuaj pleev ib ce, yog ntau dua. Raws li kev nce ntxiv ntawm cov pej xeem hauv ntiaj teb, kev thov thoob ntiaj teb rau cov khoom cog qoob loo yuav tsum tau txuas ntxiv nws txoj kev loj hlob exponential [1]. Raws li qhov tshwm sim, peb twb tau ua pov thawj ntawm kev siv ntau dhau ntawm thaj chaw nyob ntawm qee yam tshuaj ntsuab thiab cov nroj tsuag muaj ntxhiab, muab lawv nyob rau hauv kev hem thawj lossis tseem ua rau lawv ploj mus [3]. Qhov kev tsis zoo no yog qhov txaus ntshai tshwj xeeb rau cov tshuajnroj tsuag, uas feem ntau yog cov tsis tshua muaj thiab endemic hom. Cov tsiaj no feem ntau loj hlob nyob rau hauv huab cua huab cua, tshwj xeeb hauv av muaj pes tsawg leeg, lossis qee qhov latitude. Lawv cov pej xeem feem ntau tau ua tus cwj pwm los ntawm qib qis ntawm kev sib txawv ntawm cov caj ces, uas ua rau cov tsiaj no tsis tshua muaj kev cuam tshuam rau caj ces erosion thiab txo lawv txoj hauv kev muaj sia nyob thaum muaj kuab paug ib puag ncig [4]. Tsis tas li ntawd, cov khoom xyaw uas tau txais los ntawm cov nroj tsuag feem ntau yog cov sib xyaw ua ke ntawm bioactivemolecules, uas tsis tuaj yeem hloov tau yooj yim los ntawm cov tshuaj sib xyaw ua ke. Txhawm rau daws cov teeb meem saum toj no, tsob nroj biotechnology tau muab cov cuab yeej, uas tuaj yeem ua kom muaj kev nyab xeeb ntau dua thiab muaj txiaj ntsig zoo ntawm cov khoom siv phytochemicals nrog cov txiaj ntsig zoo rau kev noj qab haus huv, los npog cov kev xav tau ntawm cov tshuaj pleev ib ce. Nyob rau hauv lub xyoo caum dhau los, qhov zoo ntawm cov nroj tsuag hlwb thiab cov ntaub so ntswg kab lis kev cai technologies tau lug tshawb fawb nyob rau hauv txoj kev loj hlob ntawm high-efficiency platforms rau ntau sai zus tau tej cov tshuaj tseem ceeb molecules ntawm cov nroj tsuag keeb kwm los yog forheterologous qhia ntawm kho cov proteins [5]. Niaj hnub no, muaj ntau yam khoom siv tshuaj pleev ib ce, suav nrog ob qho tib sicosmeceuticalsthiab nutri-cosmetic, uas muaj cov khoom xyaw nquag, muab los ntawm cov nroj tsuag kab lis kev cai technology. Lub hom phiaj ntawm daim ntawv no yog los tshuaj xyuas qhov kev nce qib tsis ntev los no hauv cov cuab yeej siv hluav taws xob cog qoob loo rau kev siv tshuaj pleev ib ce thiab muab cov ntsiab lus luv luv ntawm cov khoom lag luam ntawm tes kab lis kev cai-los ntawm cov khoom xyaw tshuaj pleev ib ce muaj nyob hauv khw. Cov ntaub ntawv hauv qhov kev tshuaj xyuas no tau muab los ntawm kev tshawb fawb hluav taws xob tshawb fawb ntawm cov nroj tsuag kab lis kev cai-derivedcosmetic cov khoom xyaw thiab lawv cov ntaub ntawv qhia tshwj xeeb, ua los ntawm kev tshawb fawb hauv cov vev xaib kev lag luam ntawm cov neeg muag khoom loj xws li Prospector-R search engine, SpecialChem, Cosmetic Design Europe, the Cov kws tshawb fawb tshawb fawb Scopus, ScienceDirect, Google Scholar, thiab PubMed, thiab lub tshuab tshawb fawb nrov Google. Tsis tas li ntawd, cov kauj ruam tseem ceeb hauv kev txhim kho thiab kev ua tus cwj pwm ntawm cov nroj tsuag kab lis kev cai-derived activeingredients yog sib tham nyob rau hauv cov ntaub ntawv nthuav qhia rau kev txhim kho cov khoom InnovaStemCell Calendula.

tag nrho cistanche cag

2 Cog Extracts ua cov khoom xyaw nquag hauv tshuaj pleev ib ce

Nroj tsuagyog ib qhov chaw nplua nuj ntawm suav tsis txheeb cov metabolites uas muaj peev xwm siv tau hauv cov khoom kom zoo nkauj. Txij li thaum lub sij hawm immemorial, noob neej tau siv cov nroj tsuag sib txawv thiab lawv cov extracts los tsim cov tshuaj pleev ib ce nrog lub hom phiaj ntawm kev tsim kom muaj lub xeev ntawm cov hluas nyob mus ib txhis [6] .Tam sim no, cov nroj tsuag extracts tau dhau los ua cov khoom xyaw nrov tshaj plaws ntawm cov tshuaj pleev ib ce vim qhov kev xav tau ntau ntxiv rau ntuj. cov tebchaw uas ntxiv rau qhov zoo nkauj tuaj yeem muab cov txiaj ntsig kev noj qab haus huv ntxiv. Kev loj hlob ntawm cov khoom zoo li no, hu ua "cosmeceuticals", qhia txog qhov tsis ntev los no ntawm cov tshuaj pleev ib ce niaj hnub thiab kev lag luam tu tus kheej. Tseeb, cov nroj tsuag muaj cov khoom noj khoom haus endogenous bioactive metabolites nrog cov khoom siv tshuaj pleev ib ce thiab tshuaj [7-9]. Feem ntau ntawm cov phytochemicals, xws li polyphenols, phenolic acids, triterpenes, flavonoids. stilbenes, steroids, carotenoids, steroidalsaponins, sterols, fatty acids,polysaccharides,qab zib, peptides, thiab lwm yam, tuaj yeem muab rho tawm nrog cov kuab tshuaj tsim nyog thiab siv cov khoom xyaw asactive tam sim no hauv cov tshuaj pleev ib ce [10]. Vim li no, cov nroj tsuag loj heev tau tshawb fawb los ntawm kev lag luam tshuaj pleev ib ce hauv kev tshawb nrhiav cov khoom xyaw tshiab uas sib xyaw ua ke qee cov tshuaj pharmacological, xws li antioxidant, antimicrobial, antiviral, anti-cancer, antifungal, anti-inflammatory, anti-allergy, thiab lwm yam, thiab tseem qhia tau tias muaj zog moisturizing, anti-aging, anti-wrinkle, thiab UV tiv thaiv los [11]. Txawm li cas los xij, qhov zoo thiab phytochemical profiles ntawm cov nroj tsuag rho tawm txawv nyob rau hauv ntau yam, nyob ntawm seb huab cua, av, latitude, raws caij nyoog yam, lub sij hawm ntawm sau, thiab kev tswj teb, uas yuav yog ib qho nyuaj rau standardize lawv cov dej num [12]. Kev tshawb nrhiav tshiab natural phytochemicals tau ua rau kev sib sau ntawm bioactive extracts tsis yog los ntawmnroj tsuag, tab sis kuj los ntawm nceb, algae, thiab kuj, byutilization ntawm by-products ntawm cov nroj tsuag keeb kwm [13-17]. Niaj hnub no, nrog kev loj hlob ntawm cov neeg siv khoom txaus siab rau cov khoom siv tau zoo thiab nyab xeeb, "tiam tshiab" ntawm cov khoom siv zoo bioactivephytochemicals, tsim los ntawm cov nroj tsuag kab lis kev cai thev naus laus zis, tau qhia nyob rau hauv kaum xyoo dhau los, thiab tam sim no, lawv muaj nyob rau hauv cosmeceuticals. kev ua lag luam tau tsis tu ncua ntawm qhov tshwm sim.

cistanche keeb kwm &cistanche extract

3 Cog cell culture technology: cov hauv paus ntsiab lus rau kev tsim cov khoom siv tshuaj pleev ib ce

Plant cell culture technology yog txheej txheem rau kev loj hlob ntawm plantcells nyob rau hauv nruj tswj ib puag ncig tej yam kev mob. Vim hais tias cov hlwb raug suav hais tias yog totipotent, lawv muaj peev xwm nthuav tawm tag nrho cov tshuab genetic coded nyob rau hauv lub nucleus, thiab yog li, lawv muaj peev xwm tsim tau tag nrho cov spectrum ntawm cov yam ntxwv metabolites, pom nyob rau hauv niam.nroj tsuag. Cog hlwb areamenable mus rau zoo manufacturing txheej txheem thiab canbe yooj yim propagated los ntawm kev siv loj ntim bioreactors ntawm nws tus kheej ntawm kev nyab xeeb los yog av los yog teb tswj kev coj [5, 18].Ntxiv mus, nyob rau hauv vitro kab lis kev cai cog hlwb yog yus muaj los ntawm fastgrowth, thiab muaj peev xwm mus sau ib tug loj npaum li cas ntawm uniformbiomass rau ib lub sij hawm luv luv [19, 20]. Qhov no yog qhov txiaj ntsig tseem ceeb tshwj xeeb tshaj yog rau kev tsim cov bioactivecompounds tsis tshua muaj, xws li resveratrol, paclitaxel, lossis terpenoids, uas feem ntau pom muaj nyob hauv cov nroj tsuag tsis tshua muaj thiab lawv cov kev sib cais thiab kev ua kom huv yuav tsum tau ua ntau ntawm cov nroj tsuag biomass [21-25] . Tsis tas li ntawd, cog cell kab lis kev cai thev naus laus zis muaj qhov txhim khu kev qha thiab muaj zog ntau lawm platform rau cov khoom tsis tu ncua ntawm cov kab mob tsis muaj paug, phytochemically uniformbiomass los ntawm cov tshuaj ntsuab, muaj ntxhiab, tshuaj, thiab txawm los ntawm cov nroj tsuag tsis tshua muaj thiab raug hem [26]. Txoj kev xav kom tau txais cov phytochemicals ntuj los ntawm kev siv ib puag ncig kev ruaj ntseg biosynthetic platform ua rau cov nroj tsuag kab lis kev cai txheej txheem tshwj xeeb tshaj yog txaus nyiam rau kev tsim cov khoom xyaw nquag ntxiv rau cov txiaj ntsig ntxiv "ntsuab" cov tshuaj pleev ib ce [27-30]. Nws yuav tsum tau muab sau tseg tias cov khoom xyaw ua kom zoo nkauj, tau los ntawm cov nroj tsuag kab lis kev cai thev naus laus zis tau nrov ntawm cov neeg siv khoom raws li lub npe "cog qia hlwb". Ntawm no nws yog ib qho tseem ceeb to taub tias lo lus "stem cells" siv nyob rau hauv cov kab lus no tsis yog ib txwm raug xa mus rau cov nroj tsuag qia hlwb. Feem ntau ntawm cov kab lis kev cai muaj nyob hauv cov kab lis kev cai tsim tawm yog qhov tseeb tsim los ntawm kev siv cov nroj tsuag dedifferentiated es tsis yog ntawm kev cog qoob loo ntawm cov nroj tsuag tiag tiag. Dedifferentiated plantcells tau txais los ntawm dedifferentiation ntawm cov nroj tsuag uas twb muaj lawm txawv ntawm cov ntaub so ntswg tshwj xeeb, qhov tseeb cog qia hlwb yuav tsum tsis txhob sib txawv hauv lawv lub neej. Vim hais tias tsa los ntawm dedifferentiation ntawm cov cell sib txawv, cov kab lis kev cai ntawm cov nroj tsuag dedifferentiated tuaj yeem tau txais qee qhov kev hloov pauv, cov yam ntxwv ntawm cov ntaub so ntswg uas lawv tau txais los ntawm, thiab yog li, lawv tuaj yeem ua ntau yam hauv lawv cov biosynthetic thiab kev loj hlob. Qhov no fact ua rau nws muaj peev xwm tsim yuav luag unlimited tooj ntawm plantcell kab nrog tshwj xeeb phytochemical profiles thiab loj hlob yam ntxwv txawm los ntawm tib cov nroj tsuag, siv rau lawv pib.Vim li no, cov ntsiab lus "nplooj qia hlwb", "meristems stemcells", "kab cag. cells" "rhizome stem cells", "flower stem cells", "fruit stem cells", thiab lwm yam tuaj yeem pom nyob rau hauv INCI cov npe ntawm cov khoom xyaw activecosmetic, tab sis qhov tseeb, tag nrho cov no yog hais txog dedifferentiated cog cell kab lis kev cai. Ntawm qhov tod tes, muaj cov thev naus laus zis cog qoob loo, uas tau tsim los ntawm kev cog qoob loo ntawm cov cag ntoo tiag tiag - cov cambial meristematiccells (CMC). Cov hlwb no raug cais los ntawm txheej cambial thiab tsuas yog muaj tseeb meristem qia hlwb [31, 32]. Cambial meristematiccells yog tus cwj pwm los ntawm kev loj hlob sai thiab zoo ib yam, tsis muaj kev hloov pauv ntawm epigenetic, thiab muaj peev xwm los tsim cov txiaj ntsig zoo ntawm cov metabolites theem nrab thaum kho nrog stimulating yam xws li elicitors [33]. Cov tshuab no tau siv los ntawm lub tuam txhab Kauslim "Unhwa Corp." los tsim ntau yam khoom siv tshuaj pleev ib ce, los ntawm kev siv patent-tiv thaiv qhia platformDdobyulR, tsim los ntawm kev nthuav tawm ntawm cov cell cambialmeristematic (Table 1). Lwm qhov tseeb tseem ceeb, hais txog cov noob qes siv rau cov tshuaj pleev ib ce, yog lub sij hawm "stem cells" feem ntau sib npaug siv rau cov khoom xyaw nquag, tsim los ntawm cov kab lis kev cai callus, cell suspensions, lossis cov hauv paus plaub hau. Nws yog ib qho tseem ceeb kom nkag siab hais tias cov kab lis kev cai callus yog cov nroj tsuag cog, cultured ntawm ib tug khoom nruab nrab, whereas lub cell suspension kab lis kev cai yog ib tug cog hlwb los yog me me cell aggregates cultivated undersubmerged tej yam kev mob nyob rau hauv ib tug kua nruab nrab. Ob qho tib si callus cultures thiab cell suspensions tuaj yeem muaj cov hlwb dedifferentiated lossis truestem cells. Ntawm qhov tsis sib xws, cov hauv paus plaub hau yog cov kab lis kev cai hauv lub cev, tau txais los ntawm kev hloov pauv caj ces ntawm cov nroj tsuag hlwb [23, 34]. Qee yam ntawm cov nroj tsuag kab lis kev cai thev naus laus zis-los ntawm cov khoom xyaw tshuaj pleev ib ce, tam sim no muaj nyob rau hauv kev ua lag luam raug tshuaj xyuas hauv Daim duab 1.

3.1 Cog qia hlwb

Cov nroj tsuag tuaj yeem nthuav tawm thiab siv tau rau cov khoom siv txuas ntxiv ntawm cov nroj tsuag tshiab biomass rau cov qauv tshuaj pleev ib ce. Txawm li cas los xij, nws tseem ceeb heev kom nkag siab tias peb tsis tuaj yeem qhia tag nrho cov nroj tsuag hauv cov khoom kom zoo nkauj thiab ua kom lawv ciaj sia raws li cov khoom xyaw nquag muaj [30,35]. Cog hlwb yog cov tseem ceeb heev toenvironmental yam, nutrient nruab nrab muaj pes tsawg leeg, osmoticand mechanical (shear) stress, gas exchange and oxygen mov, kub, light, ionic zog thiab dej cov ntsiab lus, thiab yog li, lawv muaj peev xwm ciaj sia tsis nyob rau hauv lub sij hawm ntawm cov khoom kom hniav zoo nkauj npaj los yog thaum lub sij hawm cia los yog kev siv tshuaj pleev ib ce rau ntawm daim tawv nqaij. Txawm tias qhov kev xa tawm, muaj peev xwm tswj tau thiab muab cov qia hlwb tuaj yeem tsim tau, qhov loj thiab cov qauv tshwj xeeb ntawm cov hlwb cog yuav tsis tso cai rau lawv txuas lossis nkag mus rau ntawm daim tawv nqaij. Vim hais tias ntawm tag nrho cov kev txhawj xeeb, cov nroj tsuag qia hlwb yog theej siv los ua araw khoom rau kev npaj ntau hom extracts, uas ces yuav muab tso rau hauv cov tshuaj pleev ib ce raws li active cov khoom xyaw.Txawm li cas los xij, muaj ntau yam khoom, muaj nyob rau hauv kev ua lag luam, uas yog raws li tag nrho. Cov nroj tsuag qhuav thiab cov qauv ntawm cov hlwb suav ib gram ntawm cov khoom xyaw nquag (Table 1). Cov piv txwv no yog cov tshuaj tiv thaiv kev laus CeltosomeTMEryngium Maritimum ST (raws li hiav txwv holly cell kab lis kev cai nrog 100 lab hlwb / g ntawm cov khoom xyaw nquag), thiab tyrosinase inhibitory tawv nqaij lightening active ingredient CeltosomeTM Crith niam Maritimum ST (raws li pob zeb samphire cell kab lis kev cai nrog ntau tshaj 1 billion hlwb / g ntawm active ingredient) (Table 1).

3.2 Cog qia hlwb rho tawm

Feem ntau ntawm cov khoom xyaw ua kom zoo nkauj muaj nyob, tau txais los ntawm cov kab lis kev cai ntawm tes siv thev naus laus zis tau ua lag luam nyob rau hauv daim ntawv ntawm cov khoom sib txawv (Table 1). Nyob rau hauv sib piv rau cov nroj tsuag derived extracts, theextracts tau los ntawm cov nroj tsuag cell kab lis kev cai yuav ua tau yooj yim standardized thiab zoo kawg nkaus ua raws li cov nruj kev ruaj ntseg cov kev cai uas lub high-end cosmetic khw lossi xav tau [35].Cuaj cell rho tawm tsis muaj kab mob, agrochemical, tshuaj lom. , allergens, thiab pollutants vim lawv yog tsim nyob rau hauv kev tswj tej yam kev mob, ua raws li cov txheej txheem ntawm lub zoo manufacturing xyaum. Nyob ntawm seb hom siv cov kuab tshuaj, cov nroj tsuag cov khoom siv hauv cov tshuaj pleev ib ce tuaj yeem muab faib ua liposoluble (extracted nrog roj) thiab hydrosoluble (extracted glycerin) extracts, qhuav extracts (conditionedwith maltodextrin), cog cell phab ntsa rho tawm (nplua nuj nyob rau hauv peptides thiab qab zib. ), nanoemulsions lossis suspension extracts [35]. Txawm li cas los xij, feem ntau ntawm cov extracts muaj nyob rau hauv lub hauv paus ntawm lub extraction ntawm lub hom phiaj compound los yog pab pawg neeg uas muaj feem xyuam nrog bioactive tebchaw, thiab yog li, tsis tag nrho cov kev noj qab haus huv muaj peev xwm ntawm cov extracted cog hlwb yuav siv tau. Qee qhov tshwj xeeb tuaj yeem pom, qhov twg tag nrho cov nroj tsuag hlwb tau khov-qhuav thiab hmoov rau daim ntawv thov ncaj qha hauv cov qauv tshuaj pleev ib ce (saib qee cov khoom muab los ntawm "Vytrus Biotech" thiab "Naolys", Table 1), los yog tag nrho cov cell suspensions tau, emulsified los yog encapsulated nyob rau hauv liposomal complex (saib tej yam khoom uas muaj los ntawm "Innova BM" thiab "Mibelle AG Biochemistry", Table 1) los ntawm kev siv high-pressure homogenizers.

3.3 Molecular ua liaj ua teb rau zus tau tej cov recombinantproteins

Nroj tsuagyog cov khoom lag luam zoo heev rau kev nthuav qhia cov proteins sib xyaw nrog cov khoom siv tshuaj tseem ceeb [36]. Qhov no haib recombinant protein expressiontechnique, hu ua "molecular ua liaj ua teb" tau lug siv rau zus tau tej cov tshuaj tiv thaiv [37–39], cytokines [40], thiab txawm rau zus tau tej cov proteins kho rau tib neeg siv [5, 41, 42]. classical molecular ua liaj ua teb technology yog raws li geneticmodification ntawm cov nroj tsuag rau recombinant protein qhia. Qhov no tuaj yeem pom tau los ntawm kev sib koom ua ke mus tas li ntawm txawv teb chaws genesinto tus tswv tsev DNA los tsim cov kab hloov pauv ruaj khov, lossis los ntawm kev hloov pauv hloov pauv ntawm cov nplooj tsis zoo [43]. Txawm li cas los xij, ob txoj hauv kev yuav tsum muaj kev loj hlob ntawm tag nrho cov nroj tsuag raws li kev tswj hwm ib puag ncig nruj me ntsis rau hauv cov tsev cog khoom, ua raws li cov qauv kev cai nruj [36]. Los ntawm kev siv cov thev naus laus zis zoo li no, Lub Tuam Txhab Iceland "BIOEFFECT" tau thov thawj zaug kev cog qoob loo transgenic platform rau loj-scale zus tau tej cov Epidermal Growth Factors (EGF) rau kev siv tshuaj pleev ib ce, qhia nyob rau hauv genetically engineered barleyseeds. Lub tuam txhab muaj ntau yam ntawm cov khoom tu tawv nqaij uas muaj cov cellular activator, uas ua rau muaj kev noj qab haus huv thiab zoo nkauj ntawm daim tawv nqaij. Txawm li cas los xij, kev txaus siab tsis ntev los no kev ua liaj ua teb inmolecular tau tsom mus rau kev hloov pauv ntawm cov kab lis kev cai cog qoob loo rau kev tsim cov recombinantproteins. Cov txheej txheem no muaj kev ruaj ntseg thiab txuas ntxiv ntawm cov protein ntau ntau los ntawm cov nroj tsuag cog qoob loo uas tsis muaj kev tswj hwm micro-ib puag ncig hauv vitro hauv vitro hauv bioreactors. Kev cog qoob loo ntawm noob caj noob ces cog cellcultures tau lees paub tias yog lub platform muaj zog ntau dua, thaum piv rau cov txheej txheem kev ua liaj ua teb classical molecular, vam khom rau kev ua liaj ua teb-teev ntau lawm los ntawm kev loj hlob ntawm cov noob caj noob ces [18, 41]. Tsis ntev los no, lub tuam txhab Kauslim "Natural Bio-Materials (NBM)", tau tshaj tawm cov khoom siv tshuaj pleev ib ce uas muaj cov khoom loj hlob (Epidermal Growth Factor, Basic FibroblastGrowth Factor, Insulin-like Growth Factor-1, KeratinocyteGrowth Factor, Fibroblast Growth Factor) -7, thiab VascularEndothelial Growth Factor), qhia los ntawm recombinant mov (Oryza sativa L.) cell kab lis kev cai (Table 1). Txawm li cas los xij, txawm tias tib neeg recombinant proteins, tsim los ntawm cov thev naus laus zis no muaj qhov zoo tsis txaus ntseeg thiab muaj cov yam ntxwv ntawm qib siab purity, tsis muaj tsiaj, tsis muaj kab mob, tsis muaj kab mob, thiab tsis muaj exotoxin ntau lawm, tseem muaj qee cov neeg siv khoom uas muaj kev txhawj xeeb. siv cov khoom zoo li no vim hais tias lawv tau hais los ntawm cov kab mob hloov pauv caj ces.

4 Case Study: Pib, kev loj hlob, phytochemical profile thiab physicochemical yam ntxwv ntawm exopolysaccharides hauv INNOVA StemCellCalendula

Calendula (Calendula officinalis L.), hu ua "marigold", tau dav siv hauv tshuaj ntsuab tshuaj ntsuab thiab kho tawv nqaij.cosmeceuticalsrau kev siv tshuaj pleev ib ce [11] Lub pant tau pom tias muaj nplua nuj nyob rau hauv phenolic acids, flavonoids, triterpenes, carotenoids, aroma compounds, thiab ib tug tshwj xeeb mix ntawm polyunsaturated fattyacids [44-46]. Vim nws cov nqi kho mob siab thiab cov txiaj ntsig zoo nkauj pov thawj, lub tuam txhab Bulgarian "Innova BM" tau tsim thiab tso tawm ntawm kev ua lag luam ob cov khoom xyaw ua kom zoo nkauj zoo nkauj, raws li Calendula dedifferentiated cellculture (Table 1). Txoj kev loj hlob ntawm cov khoom no yog schematically nthuav tawm hauv daim duab 1. Cov kauj ruam thev naus laus zis suav nrog kev tshuaj xyuas ntawm calendulanroj tsuagnrog superior phytochemical profiles, xaiv, kom tsis muaj menyuam, thiab cultivation ntawm cog explants ntawm callusinduction nruab nrab, xaiv ntawm friable cell kab nrog tsim nyog phytochemical profiles, pib ua kua cell suspensionculture, thiab optimization ntawm cultivation tej yam kev mob thiab nutrientmedium muaj pes tsawg leeg. Qhov kev ua kom zoo tshaj plaws yog qhov tseem ceeb hauv peb cov thev naus laus zis vim tias muaj kev nce ntxiv hauv biosynthetic muaj peev xwm thiab khaws cov biomass ntawm cov kab uas tau xaiv tuaj yeem ua tiav (Table 2, Fig. 2). Tom qab kev ua kom zoo, cov kab xaiv tau nce mus rau qhov loj-loj cog qoob loo nyob rau hauv stirred tank bioreactor. Cov khoom raug tshem tawm ntawm tes (hlwb thiab kab lis kev cai kua) yog tom qab ntawd ua tiav los ntawm lub siab homogenizer los tsim glycerin extract (50 feem pua ​​​​wt.) lossis calendula emulsion (75 feem pua ​​wt. cell suspension) [47].Cov khoom xyaw uas tsim tawm tau raug pom tias muaj superior moisturizing, anti-wrinkle, hydrating thiab regenerative teebmeem thaum siv rau ntawm daim tawv nqaij. Cov teebmeem no yog vim muaj cov ntsiab lus siab ntawm secreted exopolysaccharides, thaum lub sij hawm cog qoob loo ntawm calendula hlwb (Fig. 2D). Cov exopolysaccharides tau raug txheeb xyuas los ntawm hom pectin. Cov seem ntawm exopolysaccharides crude muaj 879 ug / mg cov suab thaj nruab nrab thiab 50 ug / mg cov proteins.polysaccharidesthiab peptides ua rau qhov exopolysaccharide feem yuav luag zoo meej rau daim ntawv thov ua emulsifier hauv cov khoom siv tshuaj pleev ib ce rau daim ntawv thov. Lub peev xwm sib koom ua ke, thaum siv nrog lwm cov emulsifiers nrov, nrog rau lawv cov khoom emulsion stabilization muaj nyob rau hauv Table 3.polysaccharidesmuaj qhov hnyav molecular ntawm 6.7 × 104 Da thiab muaj 413 ug / mg ntawm uronic acids. Lub fullmonosaccharide muaj pes tsawg leeg ntawm exopolysaccharide feem raug txiav txim raws li: glucuronic acid (13.6 ug / mg), galacturonic acid (399.7ug / mg), qabzib (185.5ug / mg), galactose (179.9ug / mg), rhamnose (178.9 ug / mg). , arabinose (166.7 ug / mg), fucose (0.6 ug / mg) thiab mannose (4.7 ug / mg). Lub xub ntiag ntawm no exopolysaccharides feem cuam tshuam rau hauv Innova StemCellCalendula cov khoom, ua ke nrog bioactive compounds los ntawm cov ntsiab lus ntawm tes tso tawm (Table 2) ua rau cov khoom no tshwj xeeb thiab ib yam ntawm cov khoom lag luam ntawm cov khoom siv tshuaj pleev ib ce.

5 Cov lus xaus

Ua raws li qhov kev thov loj hlob zuj zus rau cov khoom lag luam zoo tshaj plaws ntawm cov nroj tsuag keeb kwm rau daim ntawv thov ua cov khoom xyaw nquag hauvtshuaj pleev ib ceformulations, cov nroj tsuag kab lis kev cai technology tau tsim muaj zog ntau lawm platforms uas yuav zoo muab tau cov neeg muas zaub 'xav tau kev pab. Peb yog cov pov thawj ntawm ib tug exponentially loj hlob tus naj npawb ntawm coj mus muag cog cell-derived cov khoom xyaw, muaj nyob rau hauv lub lag luam tshuaj pleev ib ce kev lag luam thiab ntau haiv neeg ntawm cov nroj tsuag siv hom, siv rau lawv cov ntau lawm tau nce txhua xyoo. Qhov tseeb, qhov kev txaus siab pom nyob rau hauv kev tsim cov nroj tsuag cell-derived cov khoom xyaw rau cov kev xav tau tshuaj pleev ib ce tuaj yeem cuam tshuam nrog kev ua ntej tsis ntev los no hauv kev txhim kho thiab kev lag luam ntawm cov nroj tsuag kab lis kev cai technology hauv cov teb chaws technologicallyadvanced. Tsis tas li ntawd, cov txheej txheem tsim tshiab ntawm kev kho cov noob caj noob ces, metabolite engineering, thiab hluavtaws biology tuaj yeem muaj kev cuam tshuam loj rau kev txhim kho cov txiaj ntsig thiab kev tsim cov khoom tsim kho kom zoo nkauj nrog cov haujlwm xav tau. Kev nce qib hauv kev ua liaj ua teb molecular twb tau ua rau kev lag luam tsim tawm ntawm cov tib neeg tsis tshua muaj activator peptides, cytokines, thiab kev loj hlob yam tseem ceeb, uas yog thawj kauj ruam hauv kev tsim cov khoom siv tshuaj pleev ib ce nrog lub peev xwm los txuas rau daim tawv nqaij los ntawm kev siv lub cev kho tus kheej. Txawm li cas los xij, txog tam sim no muaj ntau cov lus nug qhib, hais txog kev tswj hwm cov qauv thiab cov ntaub ntawv, kev sib koom ua ke ntawm cov txiaj ntsig kev noj qab haus huv, thiab cov txheej txheem rau kev soj ntsuam cov txiaj ntsig tshuaj, uas yuav tsum tau teb txhawm rau pab cov neeg siv khoom xaiv qhov lawv xaiv.tshuaj pleev ib cekhoom.

Cistanche extract pab cov tawv nqaij