PartⅠQi Sui Zhu Shui Plaster Inhibits AQP1 Thiab MAPK Signaling Txo Cov Mob Siab Ua Los Ntawm Cirrhotic Ascites

Abstract

1. Lub hom phiaj

Tam sim no, tsis muaj kev kho tshwj xeeb rau cirrhotic ascites hauv cov tshuaj niaj hnub no. Qi Sui Zhu Shui plaster (QSZSP) tau siv rau hauv ascites. Lub hom phiaj ntawm txoj kev tshawb no yog los tshawb xyuas cov txheej txheem ntawm kev ua ntawm QSZSP hauv kev kho mob cirrhotic ascites thiab nws txoj kev sib raug zoo nrog aquaporin 1 (AQP1).

2. Cov txheej txheem

Nees nkaum plaub nas tau muab faib ua plaub pawg, rau nas nyob rau hauv ib pawg. Cov roj carbon tetrachloride-olive roj yog txhaj rau hauv kev ua qauv. Cov pab pawg tswj hwm thiab cov qauv raug kho nrog cov dawb paug gel plaster (2 cm × 2 cm), QSZSP pab pawg neeg tsawg yog kho nrog Qi Sui Zhu Shui plaster (1 cm × 1 cm), thiab QSZSP pab pawg high-dose yog kho nrog Qi Sui Zhu Shui plaster (2 cm × 2 cm). Cov kev hloov pauv hauv lub cev hnyav thiab lub plab ncig tau raug ntsuas, cov kev hloov pauv hauv lub siab, lub raum, thiab peritoneum tau pom nyob rau hauv HE staining, biochemical indexes ntsig txog lub siab ua haujlwm tau kuaj pom, thiab cov kev hloov hauv AQP1 qhia thiab ua kom txoj hauv kev MAPK Nyob rau hauv daim siab, lub raum, thiab peritoneal cov ntaub so ntswg raug soj ntsuam nyob rau hauv IHC staining thiab Western blot.

3. Cov txiaj ntsig

Tom qab ib lub lim tiam ntawm kev txhaj tshuaj carbon tetrachloride daim siab roj, cov nas nyob rau hauv cov qauv pab pawg neeg nce lawv lub cev hnyav, thiab lub plab ncig ntawm cov qauv nas txuas ntxiv mus ntxiv nrog rau lub sij hawm. Tom qab 16 lub lis piam ntawm kev tsim kho ntawm tus qauv cirrhotic ascites, lub siab, lub raum, thiab peritoneum tau raug puas tsuaj, thiab cov qib ntshav ntawm TBiL, AST, ALT, Cr, BUN, K, Na, thiab Ca hauv cov nas muaj ntau dua ( P < 0 . Tom qab 4 lub lis piam ntawm kev kho mob, daim siab, raum, thiab peritoneal raug mob tau zoo lawm. TBiL, AST, ALT, Cr, BUN, K, Na, thiab Ca qib tau qis dua (P < 0.001) thiab ALB qib siab dua (P < 0.001) dua li cov hauv pawg qauv. ntxiv rau e protein qhia ntawm AQP1, p-ERK, p-JNK, thiab p-p38 tau pom tias muaj inhibited hauv daim siab, raum, thiab peritoneum.

4. Cov lus xaus

QSZSP inhibits cov protein qhia ntawm AQP1 thiab MAPK taw qhia txoj hauv kev hauv daim siab, peritoneum, thiab lub raum kom txo tau lub siab, lub raum, thiab peritoneal raug mob los ntawm cirrhotic ascites, yog li txo qis kev loj hlob txawv txav ntawm lub plab ncig.

Ntsiab lus

AQP1, MAPK signaling, lub siab raug mob, ascites,Cistanche ntxiv.

Nyem qhov no kom tau txaiscov txiaj ntsig ntawm Cistanche

Taw qhia

Cirrhosis yog ib yam kab mob uas tshwm sim los ntawm cov kab mob pathological xws li degeneration, necrosis, regeneration, thiab fibrous cov ntaub so ntswg proliferation ntawm hepatocytes vim tsis tu ncua los yog rov ua dua ntawm ntau yam pathogenic yam nyob rau hauv daim siab cov ntaub so ntswg, feem ntau manifests li daim siab. kev ua haujlwm poob [1]. Lub ntiaj teb kev nthuav dav ntawm cirrhosis tsis paub. Kev kis tus kab mob cirrhosis hauv Tebchaws Meskas yog li ntawm 0.15 feem pua ​​-0.27 feem pua ​​[2]. 69 feem pua ​​​​ntawm cov tib neeg tsis paub tias lawv muaj mob cirrhosis, thiab ntshav qab zib, haus cawv, kab mob siab C thiab kab mob siab B, ua txiv neej, thiab laus dua yog txhua yam cuam tshuam nrog cirrhosis [3]. Tsis hais txog ntawm etiology, 50 feem pua ​​​​ntawm cov neeg mob ntshav siab yuav tsim cirrhotic cardiomyopathy [4]. Xyoo 2017 National Vital Statistics Report rau Tebchaws Meskas tau tshaj tawm tias kwv yees li 4.5 lab tus neeg laus, lossis 1.8 feem pua ​​​​ntawm cov neeg laus, muaj kab mob siab ntev thiab mob cirrhosis. 41,473 tus neeg tuag los ntawm kab mob siab ntev thiab cirrhosis [5].

Ascites yog qhov tseem ceeb tshaj plaws kev kho mob tshwm sim ntawm cirrhosis. Nws yog cov kua dej txawv txav hauv lub plab kab noj hniav. Kwv yees li ntawm 60 feem pua ​​​​ntawm cov neeg mob uas tau them nyiaj rov qab tuaj yeem tsim cov kua dej peritoneal sib txawv nyob rau hauv 10 xyoo ntawm kev kuaj mob siab thiab tsis zoo ntawm cov kab mob cirrhotic ascites, tshwj xeeb tshaj yog cov ascites refractory, tuaj yeem ua rau muaj ntau yam teeb meem tuag taus, uas cuam tshuam rau lub neej zoo thiab kev ciaj sia ntawm cov neeg mob cirrhotic. [7]. 90 feem pua ​​​​ntawm cov mob qog nqaij hlav hauv siab yog cuam tshuam nrog kev muaj mob cirrhosis [8].

Cov tshuaj suav tshuaj suav tshuaj (TCM) muaj cov txiaj ntsig ntawm kev thaiv lossis ncua kev mob qog noj ntshav thiab muaj toxicity tsawg [9]. Kev siv cov tshuaj suav ntev ntev tuaj yeem txo qhov kev pheej hmoo ntawm cirrhosis hauv cov neeg mob uas muaj kab mob siab B [10]. Qi Sui Zhu Shui plaster (QSZSP) muaj Radix Astragali, Radix Kansui, Radix Aucklandiae, Poria, Herba Leonuri, Radix Salviae miltiorrhizae, thiab Pericarpium Arecae. Kev tshawb fawb soj ntsuam yav dhau los pom tias QSZSP adjuvant kev kho mob ntawm cov neeg mob cirrhotic ascites ua rau cov zis tso zis zoo dua, 24 teev tso zis sodium, txo creatinine, thiab tag nrho cov qib bilirubin, nce albumin cov ntsiab lus, txhim kho ascites rov qab tus nqi tom qab 3 lub hlis ntawm kev kho mob txuas ntxiv nrog QSZSP. [11]. Muaj qee qhov kev tshawb fawb txog QSZSP thiab cirrhotic ascites. Hauv keeb kwm ntawm kev tshawb fawb ntawm cov khoom hauv QSZSP thiab cirrhosis ntawm daim siab, Xiaozhang Tie ua ib qho kev kho rau thawj txoj kev kho mob cirrhotic ascites muaj kev nyab xeeb [12]. Salviae miltiorrhizae txo qis kev cirrhosis thiab portal hypertension [13]. Cov kev tshawb fawb ntxiv yog xav tau ntawm cov neeg ua haujlwm ntawm QSZSP hauv kev kho mob cirrhotic ascites.

channel, thiab tom qab ntawd, CHIP28 tau hloov lub npe aquaporins [14, 15]. Kaum peb aquaporins (AQP0-AQP12)) tau txheeb xyuas [16–18]. Aquaporins muaj peb isoforms. Thawj subtype thauj tsuas yog dej molecules, qhov thib ob subtype thauj cov dej molecules, urea, glycerol, thiab lwm yam, thiab thib peb subtype thauj channel txuas ntxiv qhib, tsis muaj kev siv hluav taws xob lossis kev tswj hwm [19–21]. Aquaporin 1 (AQP1) belongs rau thawj subtype, uas tshwm sim raws li ib tug tetramer thiab tso cai rau tsuas dej molecules thauj mus rau lub membrane, thiab nws yog ib tug ywj siab dej channel [22, 23]. Lub siab, peritoneum, thiab lub raum yog cov kabmob uas nyob ze, thiab AQP1 tshwm sim rau hauv daim siab, peritoneum, thiab lub raum [24]. Cov dej ntsiab lus ntawm tib neeg lub cev yog 70 feem pua ​​​​ntawm lub cev qhov hnyav, thiab cov dej tsis zoo tsim-nqus-excretion nyob rau hauv peritoneal kab noj hniav yog ib qho tseem ceeb hauv kev tsim cov ascites [19]. AQP1 yog ib qho ntawm cov aquaporins koom nrog hauv kev thauj cov dej molecules nkaus xwb. Ascites nyob rau hauv cirrhosis ua rau muaj zog tsub zuj zuj ntawm cov kua nyob rau hauv daim siab, thiab cov kua ntau dhau mus rau lub peritoneum, uas tsis reabsorb txaus dej thiab nws thiaj li ua rau kom muaj zog reabsorption ntawm lub raum, yog li AQP1 tej zaum yuav yog ib qho tseem ceeb nruab nrab ntawm kev kawm. metabolism ntawm ascites.

Thaum pib, cirrhosis ua rau lub siab ua haujlwm tsis zoo thiab ua kom cov kua dej ntau ntxiv hauv lub plab thiab cov kab noj hniav, ua rau ascites [25]. Ascites tshwm sim feem ntau hauv cirrhosis [6]. Ascites yog cov kua dej txawv txav hauv lub plab kab noj hniav [6]. Cirrhotic ascites tuaj yeem ua rau lub raum tsis ua haujlwm [26] Nyob rau hauv peripheral arterial vasodilation hypothesis, lub raum koom nrog rau tag nrho 5 theem ntawm ascites [27]. Los ntawm kev sib txuas AQP1 kev qhia hauv daim siab, peritoneum, thiab lub raum, peb tawm tswv yim "siab-peritoneal-mob" axis hauv cirrhotic ascites thiab qhia tias nws ua lub luag haujlwm tseem ceeb hauv kev tsim cov cirrhotic ascites. Nws tau raug tshaj tawm tias AQP1 yog tswj los ntawm MEK1/2 inhibitors hauv glial hlwb [28]. Hauv cov qog nqaij hlav cancer prostate, AQP1 protein qhia qib tau tswj hwm los ntawm p38MAPK [29]. Hauv tib neeg pleural mesothelial hlwb, peptidoglycan inhibits p38 MAPK kev tswj hwm ntawm AQP1 protein qhia [30]. Yog li ntawd, txoj kev MAPK yuav yog ib qho kev taw qhia ntawm AQP1 kev hloov pauv hauv "siab-peritoneal-mob raum" axis.

Lub luag haujlwm ntawm QSZSP hauv kev kho mob cirrhotic ascites thiab nws txoj kev sib raug zoo nrog AQP1 tseem tsis tau tshaj tawm. Peb xav tias "lub siab-peritoneal-lub raum" axis yog lub luag haujlwm axis ntawm dej metabolism nrog aquaporins raws li cov khoom siv hauv paus, uas koom nrog hauv "tsim-absorption-excision" ntawm peritoneal kua thiab ua lub luag haujlwm tseem ceeb hauv kev tsim. ntawm cirrhotic ascites. Txoj hauv kev MAPK tuaj yeem kho QSZSP txoj cai ntawm AQP1. Yog li ntawd, hauv txoj kev tshawb no, peb tau kho cov nas nrog cirrhotic ascites hauv vitro nrog QSZSP, tau soj ntsuam cov kev hloov pauv hauv lub siab, lub raum, thiab peritoneum, thiab ntsuas cov kev hloov pauv hauv AQP1 qhia thiab ua kom txoj hauv kev MAPK hauv lub siab, lub raum, thiab peritoneal. cov ntaub so ntswg kom elucidate cov nyhuv ntawm QSZSP hauv kev kho mob cirrhotic ascites thiab nws cov txheej txheem.

Cistanche extract

Khoom siv thiab cov txheej txheem

1. Regents.

Cov reagents siv yog raws li nram no: carbon tetrachloride (C805332) los ntawm Macklin, txiv roj roj los ntawm Yihai Kerry Golden Dragon Fish Cereals, Roj thiab Foodstuffs Co., Ltd. (Tuam Tshoj), xylene thiab absolute ethanol los ntawm National Pharmaceutical Group Chemical Reagent Co., Ltd. (Tuam Tshoj), eosin (E8090) thiab nruab nrab resin (G8590) los ntawm Solarbio (Tuam Tshoj), thiab hematoxylin (G1004) los ntawm Kev Pabcuam.

2. Qi Sui Zhu Shui Plaster txheej txheem.

Qi Sui Zhu Shui plaster muaj Radix Astragali, Radix Kansui, Radix Aucklandiae, Poria, Herba Leonuri, Radix Salviae miltiorrhizae, thiab Pericarpium Arecae. Qhov saum toj no xya cov tshuaj suav tau yuav los ntawm Thawj Tsev Kho Mob ntawm Guangxi University of Traditional Chinese Medicine, los ntawm cov kev cai ntawm "Suav Pharmacopoeia" 2010 tsab.

12 ml ntawm dej yog ntxiv rau txhua 1 g ntawm Radix Astragali, Poria, thiab Herba Leonuri thiab tsau rau 3 0 min, dej reflux extraction yog rov ua dua 3 zaug, 1.5 teev txhua zaus, thiab cov extracts tau muab tso ua ke thiab lim. . 6 ml ntawm 85 feem pua ​​​​ethanol yog ntxiv rau txhua 1 g ntawm Radix Kansui, Radix Aucklandiae, Radix Salviae miltiorrhizae, thiab Pericarpium Arecae thiab soaked rau 30 min, dej reflux extraction yog rov ua dua ob zaug, 1.5 h txhua zaus, thiab cov extracts tau muab tso ua ke thiab. lim. Cov saum toj no ob cov extracts tau muab tso ua ke thiab ziab hauv qab lub tshuab nqus tsev los npaj cov hmoov qhuav. Lub hauv paus muaj 60 feem pua ​​​​Sodium acrylate, 30 feem pua ​​​​carbomer, 10 feem pua ​​​​Aluminium glycinate, thiab 6 zaug Glycerine. Qhov kev nkag mus tau zoo muaj 50 feem pua ​​​​Azone thiab 50 feem pua ​​​​propylene glycol. Lub hauv paus, nkag mus ua kom zoo dua, thiab cov hmoov qhuav yog sib xyaw. Coater (RK-200, Tianjin Ruikang Babu Pharmaceutical Biotechnology Co., Ltd.) siv QSZSP. Cov tshuaj hydrogel yog npaj los ntawm RK-200 hydrogel txheej tshuab (Tianjin Ruikang Babu Pharmaceutical Biotechnology Co.). Txhua ntsiav tshuaj ntawm cov khoom no muaj Astragalus methylase (C41H68O14) hauv Radix Astragali, tsis tsawg dua 0.6932 mg, thiab Euphorbia grandis dienol (C30H50O) hauv Radix Kansui, tsis tsawg dua 1.4766 mg.

3. Tsiaj.

Tsiaj los ntawm peb lub tsev kawm Gorges, nees nkaum plaub nas, txiv neej, hnyav 200∼250 g, raug tsa nyob rau hauv qhov xwm txheej tsis muaj kab mob tshwj xeeb (tsis muaj kab mob tshwj xeeb, SPF). Qhov chaw pub mis yog 22-26 degree, 50% -60 feem pua ​​ntawm cov av noo. Kev ua qauv yuav pib hauv ob lub lis piam. Carbon tetrachloride-olive roj nyob rau hauv 4: 6 feem, intraperitoneal txhaj koob tshuaj ntawm 3 mL / kg lub cev qhov hnyav, thiab txhaj ob zaug ib lub lim tiam (plaub hnub thiab xya hnub) rau kaum ib lub lis piam. Rau pawg tswj hwm, kev txhaj tshuaj isotonic intraperitoneal ntawm qhov sib npaug ntawm cov roj txiv roj tau muab. Qhov hnyav thiab lub plab ncig yog ntsuas ua ntej txhua qhov kev txhaj tshuaj. Thaum lub sij hawm, cov dej distilled nrog 75 feem pua ​​​​kev kho mob cawv tau siv los npaj 10 feem pua ​​​​ntawm cov dej haus rau cov nas. Thaum kawg, hauv plaub lub lis piam ntawm kev kho mob, lub cev qhov hnyav (Table 1), thiab lub plab ncig (Table 2) ntawm cov nas raug ntsuas ob zaug hauv ib lub lis piam rau 15 lub lis piam.

4. Kev kho thiab kuaj.

Nees nkaum plaub nas tau muab faib ua plaub pawg, rau nas nyob rau hauv ib pawg, tag nrho cov nas tau dehaired ntawm lub plab, thiab dehairing cheeb tsam yog 3 cm × 3 cm. Cov pab pawg tswj hwm (Con) yog kho nrog dawb gel plaster (2 cm × 2 cm); pawg qauv (Mod), dawb paug gel plaster (2 cm × 2 cm); QSZSP pab pawg tsawg, Qi Sui Zhu Shui plaster (1 cm × 1 cm); thiab QSZSP high-dose pawg, Qi Sui Zhu Shui plaster (2 cm × 2 cm). Cov no tau siv rau hauv plab ntawm daim tawv nqaij ntawm cov nas depilated ib hnub ib zaug rau 6 h rau 4 lub lis piam. Cov roj carbon tetrachloride-olive roj tau txhaj tshuaj subcutaneously rau sab nraub qaum ib zaug hauv ib lub lis piam thaum kho kom tiv thaiv kev thim rov qab ntawm cirrhosis. Tom qab plaub lub lis piam, cov tsiaj raug tua los ntawm tshuaj loog thiab ntshav. Yog tias cov tsiaj tsis tuag, cov tsiaj tau muab tshuaj loog kom tuag nrog pentobarbital sodium overdose (100 mg / kg). Tom qab tshuaj loog, cov tsiaj tuag tsis ua pa lossis lub plawv dhia. Txoj kev lim ntawv yog siv kom tau txais ascites. Cov ntshav los ntawm lub plab aorta yog sau thiab centrifuged, thiab cov ntshav yog sau. Tus nqi tsim nyog ntawm daim siab, cov ntaub so ntswg, thiab cov ntaub so ntswg peritoneal raug txiav thiab kho nrog 4 feem pua ​​​​ntawm paraformaldehyde. Tsis tas li ntawd, cov ntaub so ntswg ntawm daim siab, peritoneum, thiab lub raum tau muab cia rau hauv lub tub yees txias txias ntawm -80 degree. Lub siab ruaj khov, lub raum, thiab cov ntaub so ntswg peritoneal ntawm nas tau lub cev qhuav dej thiab muab tso rau hauv cov quav ciab. Cov seem tau khov ntawm −20 degree thiab txiav rau hauv thickness ntawm 3 μm, da dej yog siv los ncab cov seem, cov seem tau txuas rau slides, thiab cov seem tau khaws cia ntawm 4 degree rau thaub qab.

Cistanche hmoov

5. Hematoxylin thiab Eosin (HE) Staining.

Cov seem tau stained hauv hematoxylin tov rau 4 min, tom qab ntawd yaug nrog dej ntws rau 2 min thiab 15 s. Tom qab ntawd cov seem tau muab pov tseg nrog cov tshuaj eosin rau 3 feeb. Kev ntxuav tau ua tiav ntawm 80 feem pua ​​​​thiab 95 feem pua ​​​​ethanol, feem, rau 30 s txhua. Cov seem tau muab tshem tawm hauv xylene rau 3 s thiab mounted nrog nruab nrab balsam. Tom qab staining, cov seem tau soj ntsuam siv lub tshuab ntsuas, thiab Leica Application Suite tau siv los sau thiab txheeb xyuas qhov cuam tshuam ntawm cov qauv.

6. Kev tshuaj xyuas biochemical.

Tom qab cov qauv kuaj ntshav tau thawed ntawm 4 degree, cov qauv kuaj ntshav tau kuaj pom los ntawm Shenzhen Mindray BS420 Automatic Biochemical Analyzer, thiab TBiL, AST, ALT, ALB, Cr, BUN, K, Na, thiab Ca concentrations tau txiav txim siab nrog Shenzhen Mairui ( Shenzhen, Suav teb) Matching Biochemical Kit.

7. Immunohistochemical Staining.

Cov protein qhia ntawm AQP1 hauv daim siab, peritoneum, thiab raum cov ntaub so ntswg tau kuaj los ntawm immunohistochemistry. Cov slides tau ci ntawm 65 degree rau 1 h, soaked nyob rau hauv xylene rau 30 min, thiab hydrated nyob rau hauv gradient cawv. 1 mM Tris-EDTA buffer tov (G1203, Servicebio) tau siv rau 18 min kho ntawm 125 degree thiab 103 kPa, incubated nrog 3 feem pua ​​H2O2 (National Pharmaceutical Group Chemical Reagent Co., Ltd., Tuam Tshoj) rau 10 min, incubated nrog 10 feem pua ​​tshis serum (SL038, Solarbio) rau 30 min, thiab titrated nrog thawj antibody AQP1 (PAB45863, Bioswamp), peritoneum dilution 1: 250, thiab daim siab thiab lub raum dilution 1: 400 ntawm 4 degree C thaum hmo ntuj. Cov seem tau pauv los ntawm 4 degree mus rau chav tsev kub thiab sab laug rau 45 min. Kho nrog Max Vision TM HRPPolymer anti-Lavbit Secondary antibody (KIT{27}}, MXB) rau 60 min ntawm 37 degree . Kho nrog DAB (DA1010, Solarbio) kom txog thaum cov seem hloov xim. Soak cov seem hauv lem, hematoxylin 3 min, 75% cawv 5 min, 85% cawv 5 min, 95% cawv 5 min, 100% cawv 10 min, xylene 10 min. Nruab nrab resins foob seem. Leica Application Suite duab system tau siv los ntes cov khoom cuam tshuam ntawm cov qauv. Lub nuclei tau stained xiav nrog hematoxylin, thiab DAB pom zoo xim daj daj.

8. Western Blot.

Cov qib protein ntawm p38, p-p38, ERK1/2, p-ERK1/2, JNK1/2/3, p-JNK1/2/3, thiab AQP1 hauv daim siab, peritoneum, thiab raum cov ntaub so ntswg tau soj ntsuam los ntawm Western blot . Cov qauv ntaub so ntswg tau homogenized nyob rau hauv RIPA lysate (R0010, Solarbio) uas muaj protease inhibitor ntawm 4 degree C thiab centrifuged ntawm 12000 g rau 15 min. Qhov concentration ntawm cov proteins tau ntsuas los ntawm kev siv BCA Protein Assay Kit (PC0020, Solarbio). Proteins (20 ug) raug cais los ntawm 12 feem pua ​​​​sodium dodecyl sulfate-polyacrylamide gel electrophoresis thiab xa mus rau polyvinylidene fluoride daim nyias nyias (IPVH00010, Millipore). Cov membranes raug thaiv nrog 5 feem pua ​​​​skim mis nyuj rau 2 teev ntawm chav tsev kub hauv Tris-buffered saline thiab incubated nrog thawj cov tshuaj tiv thaiv p-ERK1/2 (ab214036), JNK1/2/3 (ab208035), p-JNK1/2/ 3 (ab76572), thiab p-p38 (ab47363) los ntawm Abcam (UK), thiab ERK1/2 (MAB37123), p38 (PAB37381), AQP1 (PAB45863), thiab GAPDH (PAB36269) los ntawm Bioswamp (Tuam Tshoj), thaum hmo ntuj degree. GAPDH antibody (PAB36269, Bioswamp) raug xaiv los ua ib qho kev siv sab hauv. Tag nrho cov thawj antibody dilution piv yog 1: 1000. Cov daim nyias nyias tau ntxuav nrog Tris-buffered saline thiab incubated nyob rau hauv tshis los tiv thaiv luav IgG theem nrab antibody (SAB43714, Bioswamp, 1: 20000) rau 2 h ntawm chav tsev kub. Immunoreactivity tau pom los ntawm cov tshuaj tiv thaiv colorimetric siv Immobilon Western HRP (WBKLS0500, Millipore). Cov daim nyias nyias tau soj ntsuam nrog Tsis Siv Neeg Chemiluminescence Analyzer (Tanon 5200, Tanon).

9. Kev txheeb xyuas qhov tseeb.

Kev txheeb cais tau ua tiav siv SPSS 23.0 software. Tag nrho cov ntaub ntawv tau nthuav tawm raws li txhais tau tias ± tus qauv sib txawv (SD). Kev sib piv ntawm ob pab pawg tau tsim los siv cov qauv t-kev sim ywj pheej. Ib txoj kev ANOVA tau siv los sib piv qhov sib txawv ntawm peb lossis ntau pawg, thiab tom qab hoc Fisher qhov sib txawv tsawg kawg nkaus (LSD) tau siv rau kev sib piv ntawm ib pawg. Cov txiaj ntsig ntawm P < 0.05 tau suav tias yog qhov tseem ceeb.

Cov teebmeem ntawm Cistanche

Cov ntaub ntawv

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Rong Zhen Zhang 1 Yin Liu 1 Min Li 2 Yong Lin Huang 3 thiab Zhen Heng Song 3

1 Department of Hepatology, Thawj Tsev Kho Mob ntawm Guangxi University of suav tshuaj, Nanning, Suav

2 Lub Tsev Haujlwm Saib Xyuas Kev Kho Mob, Thawj Tsev Kho Mob ntawm Guangxi University of suav tshuaj, Nanning, Suav

3 Department of Hepatology, Guangxi University of suav tshuaj, Nanning, Suav