Tshooj II: Kev faib cov yam ntxwv ntawm plab hnyuv muaj nyob rau hauv cov neeg mob Spleen thiab raum Yang Deficiency tom qab radical Resection ntawm theem II (siab Risk) / theem III Colon Cancer Ⅷ
Sep 30, 2024
1 Tshawb nrhiav keeb kwm yav dhau
Ye Tianshi tau thov nyob rau hauv "Clinical Guide Medical Cases" tias "rau qi ua mob rau tib neeg, thiab nws hloov raws li tus neeg. muaj zog dampness, thiab qhov phem ua rau lub qi ntau zaus. " Ntawd yog, cov neeg uas muaj yin tsis muaj peev xwm lossis hluav taws kub muaj zog tau yooj yim los ntawm kev sov thiab kub siab phem qi, thaum cov neeg muajyang deficiency los yog muaj zog yin thiab txiasyog yooj yim cuam tshuam los ntawm txias thiab damp phem qi, uas yog lub thiaj li hu ua "tib qi attracts ib leeg".Cov tshuaj kws khomob tau yooj yim ua rau tus po thiab ob lub raum ntawm tib neeg lub cev, ua rau pob txha pob txha tawg; Raws li qhov no, nws tuaj yeem suav tau tias rau cov neeg uas muaj tus po thiab lub raum tsis muaj zog, cov tshuaj khomob yuav muaj feem ntau dua.ua rau tus po thiab raum puas, ua rau cov pob txha pob txha.
Raws li txoj kev xav tias "dab tsi yog sab hauv yuav tsum tau xav txog sab nraud" ("Danxi Xinchuan"), cov kab mob sib txawv ntawm tib tus kab mob yuav tsum muaj qee qhov sib txawv thiab qhov sib txawv ntawm cov qib macro thiab micro. Qhov sib txawv no tau lees paub hauv kev tshawb fawb plab hnyuv ntawm cov neeg mob uas muaj kab mob siab B ntev [spleen thiab plab damp-heat syndrome thiab mob siab rau daim siab thiab spleen deficiency syndrome],Hypertension Yin Syndrome thiab Yang Syndrome[2], mob ntshav qab zib nephropathy damp-heat syndrome thiab non-damp-heat syndrome [3], ulcerative colitis loj plab hnyuv damp-heat syndrome thiab spleen thiablub raum yang deficiency syndrome[4]. Cov kab mob hauv plab tsis yog tsuas yog cuam tshuam nrog kev tshwm sim thiab kev loj hlob ntawm cov qog nqaij hlav hauv plab [5-7], tab sis kuj tseem tuaj yeem koom nrog hauv kev kho mob plab hnyuv mucosal los ntawm kev kho mob qog [8-111 thiab pob txha pob txha [12] . Yog li, puas muaj qee yam yam ntxwv ntawm txoj hnyuv ntawm cov neeg mob spleen thiab raum yang deficiency tom qab radical resection ntawm txoj hnyuv loj?
Qhov no yog raws li kev tshawb fawb soj ntsuam "Study on the synergistic effect and mechanism of sequential prescription of strengthening spleen and raum on adjuvant chemotherapy of colon cancer" yog ua los ntawm pab neeg qhia ntawv. Cov neeg mob uas tab tom yuav tau txais kev kho mob ntxiv tom qab radical resection ntawm txoj hnyuv loj yog siv los ua cov khoom tshawb fawb los tshawb txog qhov zoo sib xws thiab qhov sib txawv ntawm cov hnyuv ntawm cov neeg mob uas muaj tus po thiab lub raum yang deficiency tom qab radical resection ntawm cov qog noj ntshav thiab cov tsis muaj poov xab thiab lub raum yang deficiency. , thiab sim qhia qhov sib txawv ntawm qhov sib txawv ntawm tus po thiab lub raum yang deficiency tom qab radical resection ntawm txoj hnyuv loj thiab cov uas tsis muaj tus po thiab lub raum yang deficiency nyob rau hauv cov kab mob plab hnyuv.
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2 Cov ntaub ntawv kho mob thiab cov kev tshawb fawb
2.1 Kev tshawb fawb tsim
Txoj kev tshawb no siv txoj hauv kev ntawm txoj kev kawm hla ntu los sau cov ntaub ntawv dav dav thiab cov ntaub ntawv TCM syndrome ntawm cov neeg mobspleen thiab raum yang deficiencythiab cov neeg mob uas tsis muaj tus po thiab lub raum yang deficiency tom qab radical resection ntawm theem II (high-risk) / theem III txoj hnyuv loj; 16s rDNA high-throughput sequencing thev naus laus zis yog siv los txheeb xyuas cov yam ntxwv ntawm cov hnyuv flora faib rau cov neeg mob uas muaj tus po thiab lub raum yang deficiency tom qab radical resection ntawm txoj hnyuv loj.
2.2 Qhov chaw ntawm cov ncauj lus
Cov neeg mob uas tau txais kev kho mob qog nqaij hlav cancer ntawm txoj hnyuv thiab tau kuaj pom tias yog theem II (muaj kev pheej hmoo siab) / theem thiab tab tom yuav tau txais kev kho mob thawj zaug hauv qhov kev ua haujlwm (Xiyuan Tsev Kho Mob, Tuam Tshoj Academy ntawm Suav Kho Mob Sciences) thiab
cov chaw zov me nyuam los ntawm Lub Kaum Hli 2018 txog Lub Kaum Ob Hlis 2020. Raws li lub Kaum Ob Hlis 31, 2020, cov ntaub ntawv xov xwm tau nkag mus rau hauv cov ntaub ntawv tshuaj ntsuam xyuas cov ntaub ntawv tswj xyuas (cov ntaub ntawv hluav taws xob ntes, EDC) ntawm Xiyuan Tsev Kho Mob, Tuam Tshoj Academy ntawm Suav Kev Kho Mob Sciences.
2.3 Cov lus qhia txog kev ncaj ncees
Txoj kev tshawb no tau raug tshuaj xyuas thiab pom zoo los ntawm Pawg Neeg Saib Xyuas Kev Noj Qab Haus Huv ntawm chav tsev (tus naj npawb pom zoo: Xiyuan Tsev Kho Mob, Tuam Tshoj Academy of Chinese Medical Sciences 2018XLA048-2). Cov neeg kawm nkag siab thiab paub txog cov ntsiab lus tshawb fawb thiab cov phiaj xwm, thiab yeem kos npe rau daim ntawv tso cai pom zoo los koom rau hauv txoj kev tshawb no.
2.4 Cov txheej txheem kuaj mob
Kev kuaj mob qog noj ntshav yog hais txog cov txheej txheem kuaj mob ntawm "Suav Common Malignant Tumor Diagnosis and Treatment Standards" ntawm Lub Tsev Haujlwm Saib Xyuas Kev Kho Mob ntawm Ministry of Health ntawm Cov Neeg Sawv Cev ntawm Tuam Tshoj. Lub chaw kho mob tau txais kev lees paub thoob ntiaj teb TNM staging system (American Joint Committee on Cancer, AJCC, 8th edition).
2.5 TCM Syndrome diagnosis cov txheej txheem
Kev kuaj mob TCM yog hais txog National Administration of Traditional Chinese Medicine's "Eleventh Five-Year Plan" qhov tshwj xeeb tseem ceeb - kev kuaj mob thiab kev kho mob rau cov kab mob qog nqaij hlav tseem ceeb. Cov hom mob qog nqaij hlav qog nqaij hlav hauv plab yog tsib hom kev tsis txaus: daim siab-pob txha tsis sib haum xeeb, spleen deficiency thiab qi stagnation, spleen-mob yang deficiency, lub siab-lub raum yin deficiency, thiab qi thiab ntshav tsis txaus [13], raws li hauv qab no:
(1) Lub siab-spleen disharmony syndrome: mob plab thiab mob hauv cov tav, plab hnyuv thiab tsis qab los noj mov, kev nyuaj siab, cov quav xoob, los yog mob plab thiab cov quav mos, qhov mob zoo tom qab zawv plab, thiab stringy mem tes.
(2) Spleen deficiency thiab qi stagnation syndrome: mob plab thiab mob hauv plab thiab tav, belching, hiccups, acid regurgitation, kev nyuaj siab, tsis qab los noj mov, nyias daj nplaig txheej, thiab stringy mem tes.
(3) Pleen thiab lub raum yang tsis muaj peev xwm: ntshai tsam khaub thuas, mob caj dab, mob daj ntseg, mob nraub qaum, mob plab, mob plab, mob plab, lossis plab zom mov, lossis edema thiab oliguria, daj ntseg thiab rog tus nplaig nrog dawb thiab nplua txheej, thiab sib sib zog nqus thiab tsis muaj zog. mem tes.
(4) Lub siab thiab lub raum yin deficiency: kiv taub hau thiab tinnitus, khaus khaus, hnyav lub taub hau thiab lub teeb taw, mob nraub qaum, nquag npau suav thiab spermatorrhea, tus nplaig liab nrog me ntsis txheej, thiab stringy thiab zoo mem tes.
(5) Qi thiab ntshav tsis txaus: qaug zog, ua tsis taus pa thiab qaug zog, daj ntseg lossis salllow complexion, kiv taub hau, daj ntseg daim di ncauj thiab rau tes, palpitations thiab insomnia, unformed quav los yog qhov quav prolapse, daj ntseg nplaig thiab tsis muaj zog mem tes.
2.6 Cov txheej txheem suav nrog thiab cais tawm
2.6.1 Cov txheej txheem suav nrog
(1) Cov neeg mob uas muaj kev kuaj pom tseeb ntawm kev mob qog noj ntshav; pathological resection margins yog qhov tsis zoo.
(2) TNM theem III (siab txaus ntshai) thiab IIIII.
(3) Cov neeg mob uas tab tom yuav tau txais CapeOX chemotherapy nyob rau hauv 6 lub lis piam tom qab radical resection ntawm txoj hnyuv loj.
(4) Tsis muaj kev koom tes hauv lwm qhov kev sim tshuaj kho mob hauv 30 hnub ua ntej randomization.
(5) Hnub nyoog 18-75 xyoo, poj niam txiv neej tsis txwv; ECOG qhab nias yog 0-1; cev xeeb tub, lactating los yog cov poj niam ntawm cov me nyuam muaj hnub nyoog yog tsis tsim nyog rau suav nrog. Cov poj niam uas muaj hnub nyoog yug me nyuam tsis tsim nyog tshwj tsis yog lawv muaj kev tiv thaiv zoo.
(6) Tsis muaj cov qog nqaij hlav qog nqaij hlav hauv lwm qhov ntawm lub cev (tshwj tsis yog rau kev kho mob ncauj tsev menyuam hauv plab, basal lossis squamous cell carcinoma ntawm daim tawv nqaij, lossis lwm cov qog uas tau kho los ntawm kev phais thiab tsis rov zoo li tsawg kawg 5. xyoo).
(7) Cov ntaub ntawv kuaj ntshav: cov ntshav niaj hnub: WBC Ntau dua lossis sib npaug ntawm 3.5 × 109 / L; NEUT Ntau dua lossis sib npaug 1.5 × 109 / L; PLT Ntau dua lossis sib npaug ntawm 100 × 109 / L; HGB Ntau dua lossis sib npaug li 90 g / L; lub siab thiab lub raum ua haujlwm: TBIL Tsawg dua lossis sib npaug ntawm 1.5 × ULN; AST, ALT Tsawg dua lossis sib npaug ntawm 2.5 × ULN; Scr Tsawg dua lossis sib npaug ntawm 1.5 × ULN; CEA: 0-5.
(8) Kos npe rau kev pom zoo.
(9) TCM Syndrome sib txawv suav nrog cov poov xab thiab lub raum yang tsis muaj peev xwm, daim siab thiab cov poov xab tsis sib haum, spleen deficiency thiab qi stagnation, daim siab thiab lub raum yin deficiency, thiab qi thiab ntshav tsis txaus. Lub siab thiab spleen disarmony, spleen deficiency thiab qi stagnation, lub siab thiab lub raum yin deficiency, thiab qi thiab ntshav deficiency yog cais raws li cov tsis muaj poov xab thiab raum yang deficiency.
2.6.2 Cov txheej txheem cais tawm
(1) Cov neeg mob uas txiav txim siab los ntawm cov kws tshawb fawb lossis cov kws kho mob tsis xav tau CapeOX chemotherapy.
(2) Cov neeg mob uas mob plawv mob hnyav, mob cerebrovascular teeb meem, kab mob siab ua haujlwm, thiab mob siab heev thiab lub raum tsis ua haujlwm.
(3) Txhua yam tsis ruaj tsis khov lossis tej yam kev mob uas yuav ua rau muaj kev nyab xeeb ntawm cov neeg mob thiab kev ua raws li txoj kev tshawb no, xws li tus mob schizophrenia thiab lwm yam mob puas hlwb.
(4) Cov neeg mob colostomy.
2.7 Kev sau thiab kuaj cov qauv
2.7.1 Kev sau cov qauv
Ua ntej pib siv tshuaj kho mob adjuvant, cov kws tshawb fawb yuav qhia txog qhov tseem ceeb ntawm txoj kev tshawb no rau cov kev kawm, kos npe rau daim ntawv tso cai pom zoo, thiab faib cov MGIEasy cov quav coj mus kuaj. 1-2 hnub ua ntej pib qhov kev kho mob thawj zaug, cov kev kawm tau sau cov qauv raws li cov lus qhia coj mus kuaj thiab muab cov quav tso rau cov kws tshawb fawb raws sijhawm.
Cov kws tshawb fawb tau coj cov qauv, kuaj xyuas thiab txheeb xyuas cov npe ntawm cov ncauj lus, muab tus lej piv txwv, thiab thauj mus rau Shanghai Meiji Biomedical Technology Co., Ltd. rau kev sim.
2.7.2 Txoj hnyuv plab 16s rDNA sequencing txheej txheem
(1) DNA extraction thiab PCR amplification
① Siv cov khoom siv EZNAQ av (Omega Bio-tek, Norcross, GA, US) kom rho tawm tag nrho DNA; NanoDrop2000 thiab 1% agarose gel electrophoresis tau siv los kuaj DNA concentration thiab purity, thiab DNA extraction zoo, feem.
② Siv 338F (5'-ACTCCTACGGGAGGCAGCAG-3') thiab 806R (5'- GGACTACHVGGGTWTCTAAT-3') primers rau PCR amplify V3-V4 cheeb tsam sib txawv. Cov txheej txheem tshwj xeeb yog:
a. 95 degree pre-denaturation rau 3 min, 27 cycles (95 degree denaturation → 55 degree annealing → 72 degree extension)
b. 72 degree txuas ntxiv rau 10 min (PCR ntsuas: ABI GeneAmpR9700).
Ntawm lawv, lub tshuab amplification muaj xws li: 20 μL, 4μL5 * FastPfu tsis, 2 μL2.5mM dNTPs, 0.8 μL primer (5 μM), 0.4 μL FastPfu polymerase thiab 10 ng DNA template .
(2) Illumina Miseq sequencing
① Siv 2% agarose gel kom rov qab tau cov khoom PCR, siv AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) rau purification, Tris-HC1 elution, thiab 2% agarose electrophoresis nrhiav kom tau;
② Siv QuantiFluorTM-ST (Promega, USA) txhawm rau txheeb xyuas thiab ntsuas kom muaj nuj nqis;
③ Xa mus rau cov txheej txheem kev khiav hauj lwm ntawm Illumina MiSeq platform (Illumina, San Diego, USA) los tsim ib lub tsev qiv ntawv PE 2 * 300 ntawm cov khoom ua kom huv si.
Cov kauj ruam tsim tsev qiv ntawv:
a. Txuas "Y"-shaped adapters;
b. Siv cov hlaws sib nqus los tshuaj xyuas thiab tshem tawm cov khoom sib txuas ntawm tus kheej;
c. Siv PCR amplification los txhawb lub tsev qiv ntawv template; d. Denature nrog sodium hydroxide los tsim ib leeg-stranded DNA fragments.
Siv IIlumina's Miseq PE300 platform rau sequencing, thiab upload cov ntaub ntawv nyoos rau NCBI database.
(3) Kev ua cov ntaub ntawv
Thawj qhov sib txuas ua ke tau zoo tswj tau siv Trimmomatic software thiab spliced siv FLASH software, raws li hauv qab no:
① Teeb lub qhov rais 50bp. Yog tias tus nqi nruab nrab zoo hauv lub qhov rais yog tsawg dua 20, txiav tawm ntawm qhov kawg ntawm lub qhov rais.
Tshem tawm tag nrho cov kab ke tom qab lub hauv paus, thiab tom qab ntawd tshem tawm cov kab ke nrog qhov ntev ntawm tsawg dua 50 bp tom qab tswj tau zoo;
② Splice cov sequences ntawm ob qho kawg raws li lub hauv paus sib tshooj. Qhov siab tshaj qhov tsis sib haum xeeb ntawm kev sib tshooj thaum sib txuas yog 0.2, thiab qhov ntev yuav tsum siab dua 10 bp.
③ Faib cov kab ke mus rau hauv txhua tus qauv raws li barcodes thiab primers ntawm ob qhov kawg ntawm qhov sib lawv liag. Siv UPARSE software (version 7.1) los pab pawg ua ntu zus rau hauv OTUs (kev ua haujlwm taxonomic units) ntawm qhov zoo sib xws ntawm 97% [14], thiab tshem tawm ib ntus thiab chimeras thaum lub sijhawm ua pawg. Siv RDPclassifier (http://rdp.cme.msu.edu/) los piav qhia txog kev faib hom rau txhua ntu.
2.8 Kev txheeb cais
(1) SPSS26. Cov ntaub ntawv ntau tau nthuav tawm raws li qhov txhais tau tias ± tus qauv sib txawv (x ± s). Rau kev sib piv ntawm pab pawg, t-test tau siv rau cov ntaub ntawv faib tawm ib txwm; tsis yog-parametric test yog siv rau cov ntaub ntawv uas tsis yog ib txwm faib; thiab chi-square test tau siv los suav cov ntaub ntawv. P<0.05 was considered statistically significant.
(2) Bioinformatics tsom xam: Qhov Loj Biotech Cloud Platform https://cloud.majorbio.com tau siv los ua hom lus piav qhia thiab kev tshuaj ntsuam xyuas, kev txheeb xyuas hom tsiaj, kev sib piv piv txwv, kev txheeb xyuas hom sib txawv, thiab lwm yam.
3 Kev tshwm sim
3.1 Cov ntaub ntawv yooj yim
A total of 32 subjects were included in the study for analysis, including 11 patients with spleen and kidney yang deficiency and 21 patients without spleen and kidney yang deficiency. The specific age, gender and primary site of the disease are shown in Tables 2-1, 2-2 and 2-3. There was no statistical difference between the two groups in terms of age, gender composition and distribution of primary sites of colon cancer (P> 0.05).
Table 2-1 Hnub nyoog faib cov neeg mob hauv ob pawg (x±s) (xyoo)
3.2 Bio cov ntaub ntawv txheeb xyuas cov txiaj ntsig
3.2.1 Alpha diversity tsom xam
Sobs index, ace index, chao index, shannon index, simpson index, etc. are commonly used measurement indicators of Alpha diversity. Among them, the first three are used to evaluate species richness (richness), the Shannon index evaluates species diversity (diversity), and the Simpson index is used to evaluate the evenness of species in the environment (evenness). This study used the Wilcoxon Rank Sum test to perform statistical analysis on the sobs index between groups. The results showed that there was no significant difference in species richness, diversity and evenness between spleen and kidney yang deficiency syndrome and non-spleen and kidney yang deficiency syndrome (P>0.05). (Table 2-4, Daim duab 2-1)
Daim duab 2-1 Qhov txawv ntawm Sobs Index ntawm pab pawg
(2) Kev soj ntsuam ntawm lub zej zog muaj pes tsawg leeg ntawm pawg
Nyob rau theem phylum, cov kab mob plab hnyuv ntawm cov neeg mob spleen-mob raum yang deficiency syndrome suav nrog: Bacteroidota 50.44%, Firmicutes 34.51%, Proteobacteria 10.81%, Actinobacteriota 1.51%, Verrucomicrobia% 1.74%, lwm tus.
Cov kab mob plab hnyuv ntawm cov neeg mob uas tsis muaj tus kab mob spleen- raum yang deficiency syndrome suav nrog: Bacteroidota 45.25%, Firmicutes 40.24%, Proteobacteria 7.86%, Actinobacteriota 3.05%, Verrucomicrobia 2.48%, lwm tus 1.13%. Nws tuaj yeem pom tau tias nyob rau theem phylum, cov kab mob ntawm ob tus po thiab lub raum yang deficiency syndrome pawg thiab pawg neeg tsis muaj poov xab thiab lub raum yang deficiency syndrome tau tswj hwm los ntawm Bacteroidetes thiab Firmicutes, suav txog li 85% ntawm tag nrho cov kab mob. . (Daim duab 2-3, Daim duab 2-4)
Daim duab 2-3 Phylum theem bar graph ntawm plab hnyuv muaj pes tsawg leeg hauv ob pawg neeg mob
Daim duab 2-4 Pie daim ntawv qhia ntawm plab hnyuv muaj pes tsawg leeg ntawm ob pawg neeg mob ntawm Phylum theem
Nyob rau theem Tsev Neeg, cov kab mob plab hnyuv muaj pes tsawg leeg ntawm cov neeg mob spleen thiab raum yang deficiency syndrome (D) thiab tsis-spleen thiab raum yang deficiency syndrome (N) raug tshuaj xyuas. Sab saum toj 5 hom kab mob nyob rau hauv tus po thiab lub raum yang deficiency syndrome pawg muaj xws li: Bacteroidaceae 37.93%, Lachnospiraceae 12.13%, Ruminococcaceae 6.93%, Prevotellaceae 6.02%, Enterobacteriaceae 7.18%; 5 hom kab mob saum toj kawg nkaus hauv pawg tsis-spleen thiab raum yang deficiency syndrome muaj xws li: Bacteroidaceae 34.38%, Lachnospiraceae 19.27%, Ruminococcaceae 5.15%, Prevotellaceae 5.71%, Sutterellaceae 4.18%. (Daim duab 2-5, Daim duab 2-6)
Daim duab 2-5 Histogram ntawm plab hnyuv muaj pes tsawg leeg ntawm ob pawg neeg mob ntawm Tsev Neeg
Daim duab 2-6 Pie daim duab ntawm cov hnyuv muaj pes tsawg leeg ntawm ob pawg neeg mob ntawm Tsev Neeg
Daim duab 2-7 NMDS tsom xam ntawm ob pawg ntawm cov zej zog nyob rau theem hom
3.2.4 Lefse multi-level hom sib txawv kev txheeb xyuas kev ntxub ntxaug
Txhawm rau txhawm rau tshawb nrhiav cov tsiaj ntxiv nrog qhov sib txawv ntawm hom kev nplua nuj ntawm pawg, Lefse (Linear
Kev txheeb xyuas qhov loj me) kev tshuaj ntsuam xyuas tuaj yeem siv los txheeb xyuas hom kev sib txawv ntawm pawg. Cov kauj ruam tsom xam Lefse yog raws li hauv qab no:
(1) Siv Kruskal-Wallis (KW) rank sum test txhawm rau txheeb xyuas txhua hom kab mob thiab tau txais ntau hom tsiaj raws li qhov sib txawv ntawm hom kev nplua nuj ntawm pab pawg.
(2) Wilcoxon rank sum test yog siv los ntsuam xyuas qhov sib txawv ntawm cov hom sib txawv uas tau txais nyob rau hauv kauj ruam (1) los txiav txim seb tag nrho cov subspecies converge mus rau tib theem kev faib tawm.
(3) Siv Linear Discriminant Analysis (LDA) kom tau txais cov hom sib txawv kawg.
Raws li pom hauv daim duab 2-8 thiab 2-9, Clostridia (o_Clostridia_UCG_014), Clostridiaceae (f_norank o Clostridia{ {6}}UCG_014), Clostridium (g_norank f norank o Clostridia_UCG_014), Flavobacteriales (o_Flavobacteriales ), Flavobacteriaceae (f_Flavobacteriaceae), Flavobacteria (g_norank Flavobacteriaceac), thiab lwm yam. yog cov kab mob uas muaj ntau ntxiv hauv cov hnyuv ntawm cov neeg mob spleen thiab raum yang deficiency syndrome. Cov flora no tuaj yeem yog lub luag haujlwm tseem ceeb rau qhov sib txawv ntawm cov neeg mob spleen-mob raum yang deficiency syndrome thiab cov uas tsis muaj tus kab mob spleen-mob yang deficiency syndrome.
Daim duab 2-8 LEfSe ntau theem hom hierarchy tsob ntoo
Daim duab 2-9 LDA daim ntawv qhia kev sib cais
4 Kev sib tham
Lub plab hnyuv muaj feem cuam tshuam nrog tus tswv tsev tiv thaiv kab mob [16,17] thiab kev nqus cov as-ham [18,19]. Cov tshuaj suav tshuaj suav hais tias "spleen tswj kev thauj mus los thiab kev hloov pauv", "spleen tswj hwm qhov tseeb", thiab "tus neeg saib xyuas yog lub zog qi ntawm dej thiab nplej". Lub luag haujlwm ntawm tus po npog ntau yam ntawm kev zom zaub mov, nqus, tshem tawm thiab tiv thaiv cov kab mob sab nraud. Yog li ntawd, tus po thiab lub plab yog feem ntau ze ze rau txoj hnyuv flora. Hauv ntau cov kab mob, kev sib raug zoo ntawm tus kab mob spleen deficiency syndrome thiab cov khoom siv pathological cuam tshuam nrog spleen deficiency, xws li damp kub, txias dampness, phlegm turbidity, thiab lwm yam, thiab dysbiosis tau lees paub. 121-231; spleen ntiaj teb yog ze ze rau daim siab ntoo. Huang Yuanyu's "Four Sacred Heart Sources" hais tias "... Thaum lub ntiaj teb tsis muaj zog thiab tsis tuaj yeem ncav cuag ntoo, ntoo qi raug thaiv ... kev loj hlob zuj zus. 124251; Kuj tseem muaj cov ntaub ntawv hais txog kev sib raug zoo ntawm lub raum yang deficiency syndrome1261 thiab plab hnyuv flora. Kev tshawb fawb txog kev sib raug zoo ntawm TCM syndrome thiab plab hnyuv tau txais kev saib xyuas ntau dua. Txoj kev tshawb no coj cov neeg mob uas tau txais kev phais radical rau mob qog noj ntshav thiab npaj mus rau kev kho mob postoperative adjuvant chemotherapy raws li kev tshawb fawb. Cov neeg mob tau muab faib ua ob pawg: spleen thiab raum yang deficiency syndrome thiab non-spleen thiab raum yang deficiency syndrome, thiab kev faib cov yam ntxwv ntawm plab hnyuv flora nyob rau hauv cov neeg mob uas muaj tus po thiab raum yang deficiency syndrome tau tshawb pom.
Cov txiaj ntsig tau pom tias tsis muaj qhov sib txawv ntawm cov neeg mob spleen thiab raum yang deficiency syndrome thiab non-spleen thiab raum yang deficiency syndrome nyob rau hauv cov nqe lus ntawm SOBs index, ACE index, Chao index, Shannon index, Simpson index, thiab lwm yam. , ntau haiv neeg thiab uniformity ntawm txoj hnyuv flora ntawm ob pawg neeg mob yeej ib yam.
Nyob rau theem phylum, cov kab mob plab hnyuv ntawm cov neeg mob nyob rau hauv cov poov xab thiab lub raum yang deficiency syndrome pawg thiab cov pab pawg neeg tsis muaj poov xab thiab lub raum yang deficiency syndrome suav nrog Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, thiab lwm yam, uas nyob ze rau lub plab hnyuv loj. ntawm cov neeg noj qab haus huv [27,28]. Txawm li cas los xij, muaj qhov sib txawv ntawm qhov sib piv ntawm Firmicutes rau Bacteroidetes (FBR), nrog rau FBR ntawm tus po thiab lub raum yang deficiency syndrome pawg yog 0.68 thiab FBR ntawm cov tsis-spleen thiab raum yang deficiency syndrome. pab pawg yog 0.89. Cov kev tshawb fawb tau pom tias kev hloov pauv hauv FBR cuam tshuam nrog kev rog [29], kub siab [27], ntshav qab zib [30], chim siab plob tsis so tswj [31], thiab lwm yam FBR tuaj yeem cuam tshuam nrog lub cev inflammatory xeev. Qhov sib txawv ntawm FBR ntawm tus po thiab lub raum yang deficiency syndrome pawg thiab pawg uas tsis yog-spleen thiab raum yang deficiency syndrome tej zaum yuav qhia tau hais tias lub cev muaj ntau yam inflammatory xeev.
Hauv tsev neeg, cov nroj tsuag tseem ceeb ntawm ob pawg neeg mob suav nrog Bacteroidetes, Lachnospiraceae, Verrucomicrobiaceae, thiab Prevotellaceae. Tsis tas li ntawd, tus naj npawb ntawm cov kab mob Enterobacteriaceae nyob rau hauv txoj hnyuv ntawm tus po thiab lub raum yang deficiency syndrome cov neeg mob tau siab dua, thaum cov kab mob Sutterellaceae nyob rau hauv cov tsis-spleen thiab lub raum yang deficiency syndrome yog siab dua. Enterobacteriaceae belongs rau Proteobacteria phylum thiab muaj dav nyob rau hauv tib neeg thiab ntau yam tsiaj. Nyob rau tib lub sijhawm, Enterobacteriaceae tuaj yeem ua tus kab mob kis tau zoo, thiab qhov nce ntawm lawv cov kab mob suav nrog yuav cuam tshuam nrog cov kab mob metabolic, tiv thaiv kab mob, thiab lwm yam kab mob [32,33]. Sutterellaceae kuj yog ib qho kab mob kis tau zoo [34]. Tus naj npawb ntawm cov kab mob Sutterellaceae nyob rau hauv cov hnyuv ntawm cov neeg mob uas muaj autism [35], chim siab plob tsis so tswj syndrome [36], thiab raws plab [37] tau nce.
Tsis tas li ntawd, Lefse ntau hom kev sib txawv ntawm cov kev tshawb fawb pom tau tias muaj ntau ntawm Flavobacterium thiab Clostridium hauv cov hnyuv ntawm cov neeg mob uas muaj tus po thiab lub raum yang deficiency syndrome tau nce, thiab qhov sib txawv yog qhov tseem ceeb. Flavobacterium feem ntau muaj nyob rau hauv ib puag ncig ntuj thiab kuj yog ib qho kab mob. Cov neeg mob uas tsis muaj zog tiv thaiv kab mob yuav kis tau tus kab mob Flavobacterium [38]. Yog li ntawd, nws yog ib qho kab mob uas ua rau mob hauv tsev kho mob [39]. Cov kev tshawb fawb tau pom tias tus naj npawb ntawm cov kab mob Flavobacterium nyob rau hauv cov hnyuv ntawm cov neeg mob uas mob plab plob tsis so tswj yog txo. Clostridium koom nrog hauv cov metabolism hauv cov kua tsib acids hauv txoj hnyuv [40]. Kev hloov pauv hauv nws tus lej yuav cuam tshuam nrog kev nyuaj siab [411], chim siab plob tsis so tswj [42], thiab kev tsis zoo rau kev kho mob [43].
5 Cov ntsiab lus
(1) Tom qab radical resection ntawm txoj hnyuv loj, tsis muaj qhov sib txawv tseem ceeb ntawm cov plab hnyuv loj ntawm cov neeg mob uas muaj tus po thiab lub raum yang deficiency syndrome thiab cov uas tsis muaj tus po thiab lub raum yang deficiency syndrome.
(2) Tej zaum yuav muaj qhov sib txawv hauv plab hnyuv FBR ntawm cov neeg mob spleen thiab raum yang deficiency syndrome thiab cov uas tsis muaj tus po thiab lub raum yang deficiency syndrome, thiab cov qauv loj yuav tsum tau nthuav dav ntxiv rau kev txheeb xyuas.
(3) Hauv chav haujlwm, tus naj npawb ntawm cov kab mob Enterobacteriaceae hauv cov hnyuv ntawm cov neeg mob spleen thiab raum yang deficiency syndrome tau siab dua, thaum cov kab mob Enterobacteriaceae hauv cov neeg tsis muaj poov xab thiab lub raum yang deficiency syndrome yog siab dua. Cov kab mob plab hnyuv ntau ntawm genus Flavobacterium thiab Clostridium tau nce ntxiv hauv cov neeg mob uas muaj tus po thiab lub raum yang deficiency syndrome.
Cov kev tshawb fawb yav dhau los tsis pom muaj kev sib raug zoo ntawm Enterobacteriaceae, Sarteobacteriaceae, Flavobacterium, thiab Clostridium thiab myelosuppression; cov qauv loj uas suav nrog hauv qhov kev tshawb fawb no yog txwv, thiab cov ncauj lus kho mob uas qhov kev tshawb fawb no tseem tsis tau pom dua. Nws yog tsis yooj yim sua los soj ntsuam seb puas muaj ib qho kev sib txuas ntawm tus po thiab lub raum yang deficiency syndrome thiab pob txha pob txha los ntawm kev siv tshuaj kho mob adjuvant. Txoj kev sib raug zoo ntawm cov pob txha pob txha, tus po thiab lub raum yang deficiency syndrome thiab plab hnyuv muaj nyob rau hauv cov kev tshawb fawb tom ntej.
Cov ntaub ntawv
[1] Xu Youli. Kev hloov pauv hauv plab hnyuv hauv cov neeg mob uas muaj tus kab mob siab B ntev nrog tus po thiab plab damp-heat syndrome thiab mob siab rau lub siab thiab tus po tsis muaj peev xwm thiab lawv cov koom haum nrog cov proteins uas cuam tshuam nrog methylation [D]. Chengdu University of Traditional suav tshuaj, 2019.
[2] Chen Yaj. Kev sib raug zoo ntawm Yin-Yang Syndrome ntawm kev kub siab thiab cov kab mob plab hnyuv [D]. Fujian University of Traditional suav tshuaj, 2019.
[3] Zhao Qihan. Qhov sib txawv hauv plab hnyuv ntawm cov ntshav qab zib nephropathy nrog damp-heat syndrome thiab non-damp-heat syndrome [D]. Beijing University of Traditional suav tshuaj, 2020.
[4] Chang Binglong. Qhov sib txawv ntawm cov hnyuv ntawm cov neeg mob uas muaj cov hnyuv loj damp-heat syndrome thiab spleen thiab raum yang deficiency syndrome nyob rau hauv ulcerative colitis [D]. Beijing University of Traditional suav tshuaj, 2020.
[5] Marzano M, Fosso B, Piancone E, et al. Stem cell impairment ntawm tus tswv tsev-microbiota interface hauv kab mob qog noj ntshav [J]. Cancers(Basel), 2021, 13(5).
[6] Liu W, Zhang X, Xu H, thiab al.Microbial zej zog heterogeneity nyob rau hauv colorectal neoplasia thiab nws correlation nrog mob plab carcinogenesis[J].Gastroenterology,2021 [7]Heo G,Lee Y,Im E.Interplay ntawm lub plab microbiota thiab inflammatory mediators nyob rau hauv txoj kev loj hlob ntawm mob qog noj ntshav [J].Cancers (Basel), 2021,13(4). [8]Wang C,Yang S,Gao L, thiab al.Carboxymethyl pachyman (cmp) txo cov hnyuv mucositis thiab tswj cov hnyuv microflora hauv 5-fluorouracil-kho ct26 qog-cov nas nas[J].Food Funct,2018 ,9(5):{14}}. [9] Genaro SC, Lima de Souza Reis LS, Reis SK, thiab al.Probiotic supplementation attenuates the aggressiveness of chemically induced colorectal tumorin nas [J]. Lub Neej Sci, 2019, 237(116895. [10]Golkhalkhali B, Rajandram R, Paliany AS, et al. Strain-specific probiotic (microbial cell npaj) thiab omega-3 fatty acid nyob rau hauv modulating zoo ntawm lub neej thiab inflammatory markers nyob rau hauv cov neeg mob qog nqaij hlav hauv plab: Ib qho randomized tswj
