PART 2 nthuav tawm Qhov cuam tshuam ntawm Ib puag ncig ntawm Cistanche Salsa: Los ntawm Ntiaj Teb Ecological Regionalization rau Av Microbial Zej Zog Cov yam ntxwv
Mar 03, 2022
Yog xav paub ntxiv thov hu rau:Joanna.jia@wecistanche.com
cistanche extract muaj ntau yam txiaj ntsig kev noj qab haus huv
4. Kev sib tham
Qhov zoo tshaj plaws kev loj hlob cheeb tsam ntawmC. salsa(Daim duab 2) feem ntau yog muab faib rau hauv cov tebchaws raws Txoj Kev Siv Txoj Kev Siv thiab Txoj Kev, xws li cov hauv Central Asia thiab West Asia, Mediterranean ntug dej hiav txwv, North African lub teb chaws, suav nrog Egypt thiab Libya, thiab Tuam Tshoj, Saudi Arabia, thiab Pakistan.31 Feem ntau ntawm cov teb chaws no nyob rau hauv cov suab puam climate zones thiab raug kev txom nyem av desertification loj heev.C. salsayog cov nroj tsuag uas tsis muaj yees duab tua kab mob uas loj hlob nyob rau hauv cov suab puam, suab puam steppe txoj siv sia, thiab cov chaw uas hnyav saline-alkali kev nyuaj siab ntawm qhov siab ntawm 700-2650 m.32C. salsaparasitizes cov hauv paus hniav ntawm nws cov tswv, xws li Palladium, Ceratoides, thiab Suaedas, uas ua lub luag haujlwm tseem ceeb hauv kev txhim kho ecological ib puag ncig ntawm arid regions.33C. salsaNws yog qhov tsim nyog rau kev loj hlob nyob rau hauv cov xwm txheej ntawm lub hnub ci, tsis tshua muaj dej nag, qhuav ntxov, qhov kub thiab txias, thiab kub sib txawv ntawm nruab hnub thiab hmo ntuj.34C. salsathiab nws tus tswv tsev tuaj yeem loj hlob ntawm thaj av uas tsis muaj av thiab tsis muaj dej khov, thiab lawv muaj lub luag haujlwm ntawm kev tswj dej thiab av, tiv thaiv cua thiab xuab zeb, thiab txhim kho thaj chaw suab puam. Yog li ntawd, txhawb kev cog qoob loo ntawmC. salsaNws yog ib qho tseem ceeb los txhim kho av desertification nyob rau hauv cov cheeb tsam no. Meanwhile, zoo heev edible thiab tshuaj muaj nqis ntawmC. salsatuaj yeem muab cov peev txheej rau kev txhim kho kev lag luam hauv zos. Lub ecological thiab tshuaj muaj nuj nqi ntawmC. salsamuab qhov tseem ceeb ntawm kev sib raug zoo rau kev txhawb nqa kev cog qoob loo, yog li muab lub hauv paus theoretical rau kev tswj hwm desertification ntawm Belt thiab Road lub teb chaws, kev txuag cov peev txheej ntawm cov tsiaj qus.C. salsa, thiab kev txhim kho kom ruaj khov ntawm nws cov txiaj ntsig kev lag luam.
Cov rhizosphere av microbiome ua lub luag haujlwm tseem ceeb hauv cov nroj tsuag lub neej hauv kev txhawb nqa cov nroj tsuag ciaj sia nyob rau hauv cov xwm txheej tsis zoo.35 Cov kev tshawb fawb tau tshaj tawm tias muaj cov nroj tsuag loj hlob rhizobia (PGPR) nyob rau hauv cov genus Arthrobacter, uas tuaj yeem yaj phosphate thiab hydrolyze casein, ua pov thawj nws lub peev xwm hauv kev txhawb nqa. cog ripening.36 Nws yog nitrogen-fixing rhizobium uas tuaj yeem tsim kev sib koom ua ke nrog cov nroj tsuag.37 Arthrobacter thiab ob peb Streptomyces strains degrade cov tshuaj tua kab ua liaj ua teb hauv kev sib koom ua ke, qhia tias lawv tseem ceeb heev hauv kev ua liaj ua teb.38 Sphingomonas qhov kev faib tawm thoob plaws hauv cheeb tsam. vim nws muaj peev xwm siv tau ntau yam ntawm cov organic tebchaw thiab kom loj hlob thiab ciaj sia nyob rau hauv cov zaub mov tsis tshua muaj.39 Qee cov kab mob hauv cov genus Sphingomonas yog PGPR uas tuaj yeem txhawb kev loj hlob ntawm cov nplej thiab txiv lws suav thiab tseem tuaj yeem siv los ua eco- tus phooj ywg lom neeg muaj peev xwm los ntxuav qhov chaw qias neeg thiab txhawb kev loj hlob ntawm cov nroj tsuag ntsib ib puag ncig cuam tshuam es.40,41 Bacillus tuaj yeem muaj sia nyob hauv cov xwm txheej hnyav thiab tuaj yeem loj hlob nyob rau hauv pH, qhov kub thiab txias, thiab ntsev ntau.42 Qee cov kab mob hauv Bacillus yog PGPR uas txhawb cov nroj tsuag kev loj hlob thiab inhibits av-yug cov nroj tsuag kab mob los ntawm kev tsim cov metabolites theem nrab.43 Rubrobacter txhawb nqa. kev loj hlob ntawm cov qoob loo ntawm thaj av cuam tshuam los ntawm salinity.35 Qee cov kab mob hauv Streptomyces ua raws li PGPR thiab cov kab mob ntawm cov nroj tsuag suppressor los ntawm ntau yam txheej txheem, xws li nce cov khoom noj khoom haus, suav nrog phosphorus, sulfur, hlau, thiab tooj liab, thiab tsim IAA, cytokinins. , thiab siderophores.44 Nroj tsuag cia siab rau kev sib cuam tshuam ntawm cov hauv paus hniav thiab cov kab mob kom tau txais cov as-ham, txhawb kev loj hlob, thiab tiv thaiv kev ntxhov siab sab nraud.45 Hauv cov ntsiab lus, cov kab mob hauv feem ntau ntawm cov tub ntxhais kawm nyob rau hauv cov av qhuav thiab barren ntawmC. salsatau tshaj tawm los tsim cov khoom noj khoom haus, txhawb cov nroj tsuag kev loj hlob, thiab pab cov nroj tsuag tiv thaiv kab mob. Cov tub ntxhais microbiomes saum toj no tuaj yeem siv los ua cov chiv tseem ceeb rau kev cog qoob loo ntawm kev kos duab.C. salsa. Peb yuav txheeb xyuas ntxiv txog kev cog qoob loo ntawm cov tub ntxhais microbiomes hauv cov haujlwm hauv qab no. Tsis tas li ntawd, txij li cov ntsiab lus tau txais txiaj ntsig ntawm high-throughput sequencing feem ntau nyob ntawm qib genus, cov kauj ruam tom ntej yog txhawm rau txheeb xyuas cov hom hom rau kev tshawb fawb.
nag lossis daus yog ib qho tseem ceeb ntawm ecological yam uas cuam tshuam rauC. salsakev faib tawm. Jackknife xeem cov txiaj ntsig hauv MaxEnt qauv (Table 2) tau txheeb xyuas tias nag lossis daus ntawm lub quarter qhuav tshaj plaws (bio17) muaj qhov txiaj ntsig siab tshaj plaws rau cov hom kev faib tawm kwv yees ntawmC. salsa. Cov nag lossis daus cuam tshuam rau cov nroj tsuag semiarid thiab arid deciduous nyob rau hauv cheeb tsam Kalahari ntawm South Africa.46 nag lossis daus thaum lub caij sov tshaj plaws feem ntau cuam tshuam rau kev faib tawm ntawm P. bauxite thiab P. vanity.47 Kev tshawb fawb txog kev hloov pauv huab cua hauv West Africa pom tias kev hloov pauv ntawm cov nroj tsuag faib Muaj feem cuam tshuam rau cov nag lossis daus.48 Rau cov av microbial zej zog muaj pes tsawg leeg ntawm C. salsa nrog cov ecotypes sib txawv, hom kev cuam tshuam ntawm ib puag ncig yam yog txawv. Raws li cov txiaj ntsig ntawm RDA thiab kev txheeb xyuas kev sib raug zoo, qhov tseem ceeb ntawm bioclimatic yam cuam tshuam rau cov av microbial muaj pes tsawg leeg yog qhov siab, nag lossis daus ntawm lub quarter sov tshaj plaws (bio18), qhov nruab nrab diurnal range (bio2), thiab qhov ntsuas kub ntawm lub quarter warmest (bio10). Qhov siab sib txawv tuaj yeem ua rau muaj kev hloov pauv hauv cov av organic teeb meem, yog li hloov qhov muaj pes tsawg leeg ntawm cov av microbial cov zej zog.49 Nyob rau hauv ib puag ncig arid, nag lossis daus hloov raws caij nyoog cuam tshuam rau cov av microbial biomass thiab cov zej zog muaj pes tsawg leeg.50 Cov kev tshawb fawb kuj tau tshaj tawm tias nag lossis daus thiab raws caij nyoog. Lub sijhawm txiav txim siab txog lub sijhawm thiab kev faib dej muaj rau cov nroj tsuag thiab microbial kev ua ub no nyob rau hauv cov suab puam txias steppe.51 Kev tshawb fawb ntawm cov qauv spatiotemporal ntawm cov kab mob sib txawv ntawm cov av hauv cov cheeb tsam arid tau pom tias nag lossis daus yog qhov tseem ceeb hauv kev txiav txim siab cov kev hloov ntawm cov kab mob.52 Kev tshawb nrhiav pom tias nag lossis daus ntawm lub quarter sov tshaj plaws yog qhov tseem ceeb rau ntau haiv neeg thiab cov zej zog muaj pes tsawg leeg ntawm Phytophthora hauv ntau thaj chaw ecological ntawm Australia, tshwj xeeb tshaj yog P. multivora thiab P. cinnamomi.53 Av qhov kub thiab txias cuam tshuam rau kev decomposition ntawm cov kab mob thiab yog li qhov muaj pes tsawg leeg ntawm cov av microbial zej zog. ,54 thiab kub a lso cuam tshuam txog kev hloov pauv raws caij nyoog ntawm cov kab mob hauv zej zog.55 Hauv kev xaus, nag lossis daus ntawm lub caij nyoog, kev hloov pauv kub, thiab qhov siab yog cov tsav tsheb eco uas yuav tsum tau txiav txim siab rau kev cog qoob loo ntawm cov nroj tsuag. C. salsa.
cistanchecov txiaj ntsig deserticola
Hauv kev xaus, txoj kev tshawb no yog thawj zaug tshawb nrhiav kev sib raug zoo ntawm ib puag ncig thiabC. salsalos ntawm macro-thiab micro dimensions. Cov lus xaus nram qab no tau txais. (1) Cov cheeb tsam uas tsim nyog rauC. salsaKev loj hlob feem ntau yog nyob rau hauv cov teb chaws raws Txoj Kev Siv thiab Txoj Kev, xws li Tuam Tshoj, Egypt, thiab Libya. (2) Cov tub ntxhais microbial genera (Arthrobacter, Sphingomonas, thiab Bacillus) ntawm peb ecotypes ntawmC. salsayuav luag PGPR uas tuaj yeem tsim lawv tus kheej cov as-ham. (3) Cov nag lossis daus yog ib qho tseem ceeb ntawm ecological uas cuam tshuam rau kev faib thiab av microbial zej zog muaj pes tsawg leeg ntawmC. salsa.Peb txoj kev tshawb fawb muab kev nkag siab txog kev tswj hwm kev sib raug zoo ntawm cov khoom tsim nyog ntawm C. salsa, av microbial zej zog, thiab ib puag ncig. Ntxiv mus, peb muab lub hauv paus theoretical rau kev cog qoob loo ntawm artificialC. salsa.
AUTHOR INFORMATION
Tus Sau Sau
Lin-Fang Huang - Lub Chaw Tshawb Fawb Tseem Ceeb ntawm Tsob Ntoo Suav Tshuaj Tiv Thaiv Kev Tiv Thaiv, Kev Tswj Xyuas Tshuaj Hauv Suav Teb, National Administration of Traditional suav Tshuaj, Lub Tsev Kawm Ntawv ntawm Kev Txhim Kho Tshuaj Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences, thiab Peking
Cistanche deserticola muaj ntau yam teebmeem
Cov kws sau ntawv
Xiao Sun - Lub Chaw Tshawb Fawb Tseem Ceeb ntawm Kev Tiv Thaiv Cov Tshuaj Suav Tshuaj Tiv Thaiv, Kev Tswj Xyuas Tshuaj Suav Tshuaj, Kev Tswj Xyuas Hauv Tebchaws Suav Tshuaj Kho Mob, Lub Tsev Kawm Ntawv ntawm Tshuaj Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences thiab Peking Union Medical College, Beijing 100193, Tuam Tshoj; orcid.org/0000-0001-9169- 3356
Jin Pei - Chengdu University of Traditional suav tshuaj, Chengdu, Sichuan 611137, Suav teb
Yu-lin Lin - Lub Chaw Tshawb Fawb Tseem Ceeb ntawm Kev Tiv Thaiv Cov Tshuaj Suav Tshuaj Tiv Thaiv, Kev Tswj Xyuas Tshuaj Suav Tshuaj, Lub Tebchaws Kev Tswj Xyuas Tshuaj Hauv Tebchaws Suav, Lub Tsev Kawm Ntawv ntawm Tshuaj Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences thiab Peking Union Medical College, Beijing 100193, Tuam Tshoj
Bao-li Li - Lub Chaw Tshawb Fawb Tseem Ceeb ntawm Kev Tiv Thaiv Cov Tshuaj Suav Tshuaj Tiv Thaiv, Kev Tswj Xyuas Tshuaj Hauv Suav Teb, Kev Tswj Xyuas Tshuaj Hauv Tebchaws Suav, Lub Tsev Kawm Ntawv ntawm Tshuaj Kho Mob, Suav Academy ntawm Kev Kho Mob Sciences thiab Peking Union Medical College, Beijing 100193, Tuam Tshoj
Li Zhang - College of Science, Sichuan Agriculture University, Ya'an, Sichuan 625014, Suav teb
Bashir Ahmad - Chaw rau Biotechnology & Microbiology, University of Peshawar, Peshawar 25000, Pakistan
Ua kom tiav cov ntaub ntawv tiv tauj muaj nyob ntawm: https://pubs.acs.org/10.1021/acs.jafc.0c01568
CistancheExtract muaj anti-hluav taws xobnoj qab haus huvcov txiaj ntsig
Nyiaj txiag
Txoj haujlwm no tau txhawb nqa los ntawm National Natural Science Foundation ntawm Tuam Tshoj (81473315 thiab U1812403-1), National Science & Technology Fundamental Resources Investigation Program of China (2018FY100701), Sichuan Province Science and Technology Plan Project (2018JZ0028), Qhib Kev Tshawb Fawb Fund of Chengdu University of Traditional Chinese Medicine Key Laboratory of Systematic Research of Distinctive Chinese Medicine Resources in Southwest China (003109034001), thiab Beijing Natural Scientific Foundation (7202135), uas tau lees paub ua tsaug.
Sau ntawv
Cov kws sau ntawv tshaj tawm tsis muaj kev sib tw nyiaj txiag txaus siab.
TXOJ CAI
Peb ua tsaug ntau rau Xiangxiao Meng los ntawm Lub Tsev Haujlwm ntawm Suav Materia Medica, Tuam Tshoj Academy ntawm Suav Kev Tshawb Fawb Txog Kev Kho Mob, rau cov lus qhia siv MaxEnt thiab ArcGIS software.
REFERENCES
(1) Trampetti, F.; Pereira, C.; Rodrigues, MJ; Celaj, O.; D'Abrosca, IB; Zeng, G.; Mollica, UA; Stefanucci, UA; Custod́io, L. Tshawb nrhiav cov halophyte Cistanche phelypaea (L.) Cout raws li cov khoom siv txhawb kev noj qab haus huv: Hauv vitro antioxidant thiab enzyme inhibitory zog, metabolomic profile, thiab kev tshawb fawb suav. J. Pharm. Biomed. Qhov quav. Xyoo 2019, 165, 119-128.
(2) Wiedermann, MM; Neeb, A.; Gunnarsson, UA; Nilsson, MB; Ericson, L. Kev hloov pauv thoob ntiaj teb hloov cov nroj tsuag thiab cov nroj tsuag-parasite kev sib cuam tshuam hauv cov av nkos. Ecology 2007, 88 (2), 454–464.
(3) Sakaguchi, S.; Horie, K.; Ishikawa, N.; Nishio, S.; Tsim nyog, JR; Fukushima, K.; Yamasaki, M.; Ito, M. Kev tu cov av ecotypes ntawm Solidago virgaurea nyob ze parapatry ntawm divergent flowering lub sij hawm thiab xaiv tawm tsam cov neeg tsiv teb tsaws chaw. J. Ecol. 2019, 107 (1), 418–435.
(4) Sun, X.; Li, L.; Peb, J.; Liu, C.; Huang, L.-F. Metabolome thiab transcriptome profiling qhia qhov kev hloov pauv zoo thiab cov kev cai hauv qab ntawm peb ecotypes rau Cistanche deserticola. Cog Mol. Biol. 2020, 102 (3), 253–269.
(5) Li, L. Josef, IB; Liu, B.; Zheng, S.; Huang, L.; Chen, S. Kev ntsuam xyuas peb-dimensional ntawm Ecotypic Diversity of Traditional Chinese Medicine: Ib Case Study of Artemisia annua L. Pem hauv ntej. Tsob Ntoo Sci. Xyoo 2017, 8, 1225.
(6) Li, L. Zheng, S.; Brinckmann, JA; Fu, J.; Zeng, R.; Huang, L.; Chen, S. Tshuaj thiab genetic ntau haiv neeg ntawm Astragalus mongholicus zus nyob rau hauv txawv eco-climatic cheeb tsam. PLoS Ib 2017, 12 (9), e0184791.
(7) Förster, N.; Ulrichs, C.; Schreiner, M.; ib. Arndt, N.; Schmidt, R.
Mewis, I. Ecotype variability hauv kev loj hlob thiab theem nrab metabolite profile hauv Moringa oleifera: Kev cuam tshuam ntawm leej faj thiab dej muaj. J. Agric. Khoom noj khoom haus Chem. 2015, 63 (11), 2852–2861.
(8) Xovxwm, MC; Phoenix, GK Kev cuam tshuam ntawm cov nroj tsuag kab mob ntawm cov zej zog ntuj. New Phytol. 2005, 166 (3), 737–751.
(9) Bardgett, RD; Smith, RS; Shiel, RS; Peacock, S.; Simkin, JM; Quirk, H.; Hobbs, PJ Parasitic nroj tsuag tsis ncaj nraim tswj cov khoom hauv qab hauv av hauv cov nyom nyom. Nature 2006, 439 (7079), 969– 972.
(10) Du, Z.; Wu, J.; Meng, X.; Li, J.; Huang, L. Kev kwv yees lub ntiaj teb muaj peev xwm faib tawm ntawm plaub hom kab mob Panax nyob rau hauv nruab nrab- thiab qis-latitude thaj tsam ntawm Tuam Tshoj los ntawm thaj chaw cov ntaub ntawv xov xwm rau cov nroj tsuag tshuaj ntiaj teb (GMPGIS). Molecules 2017, 22 (10), 1630.
(11) Wang, Y.; Zhang, L. Du, Z.; Peb, J.; Huang, L. Tshuaj lom neeg ntau haiv neeg thiab kev kwv yees ntawm cov peev txheej cog qoob loo ntawm Cistanche tshuaj ntsuab. Sci. Rep. 2019, 9 (1), 1–13.
(12) Moob, J.; Williams, J.; Baena, S.; Wilkinson, T.; ib. Gole, TW; Chala, ZK; Demisew, S.; Davis, AP Resilience muaj peev xwm ntawm Ethiopian kas fes sector nyob rau hauv kev hloov pauv huab cua. Nat. Nroj tsuag 2017, 3 (7), 17081.
(13) Meng, X.; Huang, L.; Dong, L.; Li, X.; Wei, F; Chen, Z.; Wu, J.; Sun, C.; yus, Y.; Chen, S. Kev tshuaj xyuas thoob ntiaj teb ecology ntawm Panax notoginseng hauv kev tsim nyog thiab zoo. Acta Pharm. Kev txhaum. 2016, 51 (9), 1483–1493.
(14) Bradie, J.; Leung, B. Ib qho kev sib xyaw ua ke ntawm qhov tseem ceeb ntawm cov khoom siv hauv MaxEnt hom kev faib cov qauv. Phau ntawv Journal of Biogeography 2017, 44 (6), 1344–1361.
(15) Ren, G.; Mateo, RG; Liu, J.; Zoo, T.; Alvarez, N.; Guisan, A.; Conti, E.; Salamin, N. Genetic txim ntawm Quaternary climatic oscillations nyob rau hauv lub Himalayas: Primula tibetica raws li cov ntaub ntawv kawm raws li txwv qhov chaw-txuas nrog DNA sequencing. New Phytol. 2017, 213 (3), 1500–1512.
(16) Ju, F.; Zhang, T. 16S rRNA gene high-throughput sequencing data mining of microbial diversity and interactions. Appl. Microbiol. Biotechnol. 2015, 99 (10), 4119–4129.
(17) Cui, J.-L.; Vijayakumar, V.; Zhang, G. Partitioning ntawm fungal endophyte assemblages nyob rau hauv paus-parasitic nroj tsuag Cynomorium songaricum thiab nws tus tswv Nitraria tangutorum. Pem hauv ntej. Microbiol. Xyoo 2018, 9, 666.
(18) Fick, SE; Hijmans, RJ WorldClim 2: tshiab 1-km spatial daws teeb meem climate surfaces for global land area. International Journal of climatology 2017, 37 (12), 4302–4315.
(19) Dormann, CF; Elith, J.; Bacher, S.; Buchmann, C.; ib. Carl, G.; Carre, G.; Marqueź, JRG; Gruber, IB; Lafourcade, IB; Leitaõ, PJ Collinearity: kev tshuaj xyuas ntawm txoj hauv kev los daws nws thiab kev simulation kev soj ntsuam xyuas lawv cov kev ua tau zoo. Ecography 2013, 36 (1), 27–46.
(20) Phillips, SJ; Dudík, M. Kev ua qauv ntawm hom kev faib tawm nrog Maxent: kev txuas ntxiv tshiab thiab kev ntsuam xyuas dav. Ecography 2008, 31 (2), 161–175.
(21) Munyaka, PM; Eissa, N.; Bernstein, CN; Khafipur, E.; Ghia, J.-E. Mo1774 Prenatal Antibiotic Treatment tsub kom cov me nyuam muaj kev cuam tshuam rau kev sim mob plab: Lub luag haujlwm ntawm lub plab Microbiota. PLoS One 2015, 10 (11), e0142536.
(22) Edgar, RC UPARSE: qhov tseeb OTU cov kab ke los ntawm microbial amplicon nyeem. Nat. Txoj kev 2013, 10 (10), 996.
(23) Cole, JR; Wang, Q.; Cardenas, E.; Ntses, J.; Chai, B.; Farris, RJ; Kulam-Syed-Mohideen, A.; McGarrell, DM; Mas, T.; Garrity, GM; ua al. Ribosomal Database Project: txhim kho kev sib raug zoo thiab cov cuab yeej tshiab rau rRNA tsom xam. Nucleic Acids Res. Xyoo 2009, 37, D141-D145.
(24) Wang, Y.; Sheng, H.-F.; Nws, Y.; Wu, J.-Y.; Jiang, Y.-X.; Tam, NF- Y.; Zhou, H.-W. Kev sib piv ntawm cov kab mob sib txawv hauv cov dej tsis qab, cov av ntub dej nruab nrab, thiab cov dej hauv hiav txwv los ntawm kev siv ntau lab ntawm illumina cim npe. Appl. Ib puag ncig. Microbiol. Xyoo 2012, 78 (23), 8264.
(25) Jiang, XT; Peng, X.; Deng, GH; Sheng, HF; Wang, Y.; Zhou,
HW; Tam, FY Illumina Sequencing of 16S rRNA Tag Revealed Spatial Variations of Bacterial Communities in a Mangrove Wetland. Microb. Ecol. 2013, 66 (1), 96–104.
(26) Sanner, MF Python: cov lus programming rau kev sib koom ua ke thiab kev txhim kho software. J. Mol. Graphics Modell. 1999, 17 (1), 57–61.
(27) Caporaso, JG; Kuczynski, J.; Stombaugh, J.; Kev, K.; Bushman, FD; Costello, UA; Fierer, N.; Pena, AG; Goodrich, JK; Gordon, IB; Huttley, GA; Kelley, ST; Knights, D.; Koenig, JE; Li, RE; Lozupon, CA; McDonald, D.; Muegge, BD; Pirrung, M.; Reeder, J.; Sevinsky, JR; Turnbaugh, PJ; Walters, WA; Widmann, J.; Yatsunenko, T.; Zaneveld, J.; ib. Knight, R. QIIME tso cai rau kev txheeb xyuas cov ntaub ntawv sib luag hauv zej zog. Nat. Txoj Kev 2010, 7 (5), 335–6.
(28) Schloss, PD; Westcott, NWS; Ryabin, T.; Hall, JR; Hartmann, M. Hollister, IB; Lesniewski, RA; Oakley, BB; Chaw ua si, DH; Robinson, CJ; ua al. Taw qhia mothur: qhib-qhov chaw, platform-ywj siab, kev txhawb nqa hauv zej zog rau kev piav qhia thiab sib piv cov zej zog microbial. Appl. Ib puag ncig. Microbiol. 2009, 75 (23), 7537–7541.
(29) Chou, J.; Liu, P.; Zhou, G.; Xia, J. Siv MicrobiomeAnalyst rau kev txheeb cais, kev ua haujlwm, thiab kev txheeb xyuas cov ntaub ntawv microbiome. Nat. Protoc. 2020, 15 (3), 799–821.
(30) Phillips, SJ; Anderson, RP; Schapire, RE Qhov siab tshaj plaws entropy qauv ntawm hom kev faib tawm thaj chaw. Ecol. Qauv. 2006, 190 (3–4), 231–259.
(31) Cai, P. Nkag siab txog Tuam Tshoj txoj siv thiab txoj kev pib; Lower Institute, 2017.
(32) Zheng, S. Jiang, X.; Wu, L.; Wang, Z.; Huang, L. Tshuaj thiab tshuaj genetic discrimination ntawm cistanches herba raws li UPLC-QTOF/MS thiab DNA Barcoding. PLoS Ib 2014, 9 (5), e98061.
(33) Liu, W. Nkauj, Q.; Cai, Y.; Xie, N.; Li, Z.; Jiang, Y.; Zheng, J.; Tu, P.; Nkauj, Y.; Li, J. Los ntawm 1H NMR-based non-targeted rau LC-MS-raws li lub hom phiaj metabolomics lub tswv yim rau qhov tob chamomile sib piv ntawm plaub hom Cistanche. J. Pharm. Biomed. Qhov quav. Xyoo 2019, 162, 16-27.
(34) Jiang, Y. Tu, P.-F. Kev soj ntsuam ntawm cov tshuaj lom neeg hauv Cistanche hom. Phau ntawv Journal of chromatography A 2009, 1216 (11), 1970–1979.
(35) Mukhtar, S.; Mirza, IB; Mehnaz, S.; Mirza, MS; Mclean, J.; Malik, KA Kev cuam tshuam ntawm av salinity ntawm cov qauv microbial ntawm halophyte rhizosphere microbiome. Ntiaj teb J. Microbiol. Biotechnol. 2018, 34 (9), 136.
(36) Ntxoov, P.; Chen, D.; Nws, Y.; Zhou, Q. Tian, Y.; Gao, L. Kev txo qis kev ntxhov siab hauv cov txiv lws suav siv cov Arthrobacter thiab Bacillus megaterium cais tawm ntawm rhizosphere ntawm cov nroj tsuag qus cog rau ntawm thaj av saline-alkaline. Int. J. Phytorem. 2016, 18 (11), 1113–1121.
(37) Gupta, G.; Parihar, SS; Ahirwar, NK; Neeb, SK; Singh, V. Cog kev loj hlob txhawb nqa rhizobacteria (PGPR): tam sim no thiab yav tom ntej kev cia siab rau kev loj hlob ntawm kev ua liaj ua teb ruaj khov. J. Microb. Biochem. Tshuab. 2015, 7 (2), 96–102.
(38) Zohar, S.; Kviatkovski, ib.; Masaphy, S. Kev ua siab ntev rau thiab degradation ntawm siab p-nitrophenol concentrations los ntawm inoculum loj manipulations ntawm Arthrobacter 4H cais los ntawm cov av ua liaj ua teb. Int. Biodeterior. Biodegrad. Xyoo 2013, 84, 80–85.
(39) Asaf, S.; Khan, AL; Khan, MA; Al-Harris, A.; Li, J.-J. Ua kom tiav genome sequencing thiab tsom xam ntawm endophytic Sphingomo- nas sp. LK11 thiab nws cov peev xwm hauv kev cog qoob loo. 3 Biotech 2018, 8 (9), 389.
(40) Kim, Y.J.; Lim, J.; Sukweenadhi, J.; Seok, JW; Li, S.-W.; Park,
JC; Taishanova, A.; Kim, D.; Yang, DC Genomic characterization of newly cais rhizobacteria Sphingomonas panacis nthuav tawm cov nroj tsuag kev loj hlob-txhim kho cov nyhuv rau mov. Biotechnol. Bioprocess Eng. 2019, 24 (1), 119–125.
(41) Khan, AL; Waqas, M.; Kang, S.-M.; Al-Harris, A.; Hussain, J.; Al-Rawahi, A.; Al-Khiziri, S.; Ullah, I.; Li, L.; Jung, H.-Y.; ua al. Cov kab mob endophyte Sphingomonas sp. LK11 tsim gibberellins thiab IAA thiab txhawb nqa cov txiv lws suav loj hlob. J. Microbiol. 2014, 52 (8), 689–695.
(42) Rüger, H.; Fritze, D.; Spröer, C. Tshiab psychrophilic thiab psychrotolerant Bacillus Marinus strains los ntawm tropical thiab polar deep-sea sediments thiab emended piav qhia ntawm hom. Int. J. Syst. Evol. Microbiol. 2000, 50 (3), 1305–1313.
(43) Gutieŕrez-Mañero, FJ; Ramos-Solano, IB; Probanza, A. N.; Mehouachi, JR; Taub, F.; Talon, M. Cov nroj tsuag-loj hlob-txhim kho rhizobacteria Bacillus pumilus thiab Bacillus licheniformis tsim muaj ntau ntawm physiologically active gibberellins. Physiol. Nroj tsuag. 2001, 111 (2), 206–211.
(44) Viaene, T.; Lanendries, S.; Beirinckx, S.; Mas, M.; Goormachtig, S. Streptomyces ua ib tsob nroj tus phooj ywg zoo tshaj plaws? FEMS Microbiol. Ecol. 2016, 92 (8), fwm 119.
(45) Edwards, J.; Johnson, C.; Santos-Medellin, C.; Lus, E.; Podishetty, NK; Bhatnagar, S.; Eisen, JA; Sundaresan, V. Structure, variation, and assembly of the root-associated microbiomes of rice. Proc. Natl. Acad. Sci. USA 2015, 112 (8), E911.
(46) Jolly, WM; Khiav, SW Qhov cuam tshuam ntawm nag lossis daus thiab av muaj peev xwm ntawm drought deciduous phenology hauv Kalahari. Ntiaj teb no Change Biology 2004, 10 (3), 303–308.
(47) Yuan, H.-S.; Wei, Y.-L.; Wang, X.-G. Maxent qauv rau kev kwv yees lub peev xwm faib ntawm Sanghuang, ib pab pawg tseem ceeb ntawm cov tshuaj fungi hauv Suav teb. Kab mob. Ecology 2015, 17, 140–145.
(48) Wittig, R.; Koj, K.; Schmidt, M.; ib. Szarzynski, J. Ib txoj kev tshawb fawb txog kev hloov pauv huab cua thiab kev cuam tshuam anthropogenic hauv West Africa. Ib puag ncig. Sci. Pollut. Res. 2007, 14 (3), 182–189.
(49) Rovner, C.; Peter, H.; Catalań, N.; Drewes, F.; Sommaruga, R.; Peŕez, MT Kev nyab xeeb ntsig txog kev hloov pauv ntawm cov yam ntxwv hauv av cuam tshuam cov kab mob hauv zej zog muaj pes tsawg leeg thiab kev ua haujlwm ntawm qhov siab siab thiab latitude pas dej. Ntiaj teb no hloov biology 2017, 23 (6), 2331–2344.
(50) Zhao, Q.; Jian, S.; Nuj, N.; Maestre, FT; Tedersoo, L.; Nws, J.; Wei, H.; Tan, X.; Shen, W. Hloov nag lossis daus raws caij nyoog cuam tshuam rau cov kab mob tseem ceeb tab sis cov kab mob tsawg tsawg hauv cov av hav zoov hav zoov. Biol. Fertil. Av 2017, 53 (2), 231–245.
(51) Sorensen, PO; Germino, MJ; Feris, KP Microbial zej zog cov lus teb rau 17 xyoo ntawm kev hloov nag lossis daus yog raws caij nyoog thiab ua ke rau kev sib txawv ntawm cov dej ntsiab lus ntawm cov nroj tsuag thiab av C. Av Biol. Biochem. 2013, 64, 155–163.
(52) Azatyan, A. Qhov cuam tshuam ntawm Hnyav los nag ntawm cov suab puam av kab mob hauv zej zog kev sib xyaw thiab kev ua haujlwm zoo; Ben Gurion University of the Negev, Jacob Blaustein Institute for Desert Research, 2013.
(53) Burgess, TI; McDougall, KL; Scott, PM; Hardy, GES; Garnas, J. Predictors ntawm Phytophthora ntau haiv neeg thiab zej zog muaj pes tsawg leeg nyob rau hauv tej yam ntuj tso chaw nyob rau ntau haiv neeg Australian ecoregions. Ecography 2019, 42 (3), 565–577.
(54) Zogg, GP; Zak, DR; Ringelberg, IB; Dawb, DC; MacDonald, NWS; Pregitzer, KS Cov Txheej Txheem thiab ua haujlwm hloov pauv hauv cov zej zog microbial vim av sov. Soil Science Society of America Journal 1997, 61 (2), 475–481.
(55) Gao, P.; Xu, W. Sontag, P.; Li, X.; Xu, G.; Liu, T.; Sun, W. Correlating microbial zej zog compositions nrog ib puag ncig yam nyob rau hauv activated sludge los ntawm plaub tag nrho-scale cov dej khib nyiab kho nroj tsuag hauv Shanghai, Suav teb. Appl. Microbiol. Biotechnol. 2016, 100 (10), 4663–4673.
