mwwXaiv lus
Tsev / Kev paub / Ntsiab lus

Kev paub

Tshooj 2 Kev Tiv Thaiv Los Ntawm Phenylethanol Glycosides Los Ntawm Cistanche Tubulosa Ntawm Bovine Serum Albumin-induced Hepatic Fibrosis hauv nas

Mar 06, 2022

Hu rau: Audrey Hu Whatsapp / hp: 0086 13880143964 Email:audrey.hu@wecistanche.com

NYEEM NO PAET 1


NF-κB p65 thiab collagen I qhia tom qab siv tshuaj cuam tshuam hauv HSC-T6 hlwb Txhawm rau ua kom pom cov cim ntawm lub siab fibrosis, ob lub ntsiab lus tseem ceeb hauv lub siab tau txiav txim siab los ntawm RT-PCR kev soj ntsuam. Cov ntaub ntawv qhia tau hais tias tHSC-T6 hlwb los ntawm pawg tswj tau qis dua ntawm NF- κB thiab collagen hom I mRNA. Conversely, TGF- 1 induced HSC-T6 hlwb kom pom zoo upregulate NF-κB (P < 0.01)="" thiab="" col-i="" (p="">< 0.01)="" mrna,="" nrog="" qib="" siab="" dua="" cov="" nyob="" hauv="" pawg="" tswj="" hwm.="" nyob="" rau="" hauv="" lub="" xub="" ntiag="" ntawm="" txawv="" concentrations="" ntawm="" cphgs="">phenylethanoid glycoside los ntawm cistanche), cov txiaj ntsig ntawm NF-κB p65 [P NF-κB=0}.001 rau 100 ug/ml, P NF-κB=0.002 rau 75 ug/ml, P NF-κB {{ 11}}.007 rau 50 ug/ml, thiab P NF-κB=0.012 rau 25 ug/ml, ntsig txog] thiab Col-I [P Col-I=0.006, P Col -I=0.009, P Col-I=0.014, P Col-I=0.019, ntsig txog] tau qhia txo qis ntawm cov mRNAs (Fig. 7).

cistanche planting

Western blot tsom xam ntawm collagen I qib tom qab siv tshuaj cuam tshuam hauv HSC-T6 hlwb Daim duab 8 qhia txog qib collagen I protein hauv HSC-T6 hlwb ntawm cov pab pawg sib txawv. Cov qib collagen I protein qhia tau txo qis hauv ntau pawg ntawm CPhGs(phenylethanoid glycoside los ntawm cistanche)(100 ug/ml, 75 ug/ml, 50 ug/ml, thiab 25 ug/ml) piv nrog rau pawg TGF- 1


image

Kev sib tham

CPhGs(phenylethanoid glycoside los ntawm cistanche)yog phenylethanoid glycoside cais thiab purified los ntawm rhizome ntawm Cistanche, uas yog siv raws li ib txwm suav tshuaj ntsuab tshuaj. Nyob rau hauv xyoo tas los no, CPhGs tau pom tias muaj peev xwm tiv thaiv lub siab raug mob [20]. Yog li ntawd, peb tsom mus soj ntsuam seb CPhGs puas muaj kev cuam tshuam rau cov kab mob siab fibrosis los ntawm BSA-induced hepatic fibrosis hauv nas. BJRG feem ntau yog siv los ua cov tshuaj kho mob rau lub siab fibrosis hauv Suav teb. Nws yog tsim los ntawm vaub kib plhaub. Radix Paeonia rubra, Cordyceps Sinensis, Radix isatidis, thiab lwm yam muaj kev cuam tshuam los ntawm kev ntxiv Qi thiab ntshav, tshem tawm qaug zog, muag muag. Tsis tas li ntawd, cov kev tshawb fawb yav dhau los qhia tau hais tias nws muaj peev xwm ua kom pom tseeb ntawm kev thaiv lub siab fibrosis thaum ntxov, inhibiting kev loj hlob ntawm cov rog-tso cov hlwb, thiab txo collagen synthesis [21]. Yog li ntawd, BJRG tau siv los ua cov tshuaj zoo hauv txoj kev tshawb no.


Cov kev hloov pauv pathological ntawm cov kab mob siab fibrosis hauv cov nas raug ntxias los ntawm kev txhaj tshuaj BSA zoo ib yam li cov neeg mob ntshav qab zib mob ntshav qab zib [22]. CPhGs(phenylethanoid glycoside los ntawm cistanche)koob tshuaj-dependently alleviated qib ntawm daim siab fibrosis thiab inhibited HSC hloov mus rau myofibroblast zoo li cov hlwb, txo cov qib siab ntawm cov ntshav ALT, AST, HA, LN, CIV, TGF- 1 thiab daim siab Performance index, thiab markedly suppressed qhia. ntawm collagen I, collagen III thiab TGF- 1 hauv daim siab. Cov theem ntawm hepatic fibrosis yog txuam nrog cov qib ntshav ntawm HA, LN thiab IV-C, uas raws li cov cim yuav ua lub luag haujlwm hauv kev txheeb xyuas qhov degree ntawm hepatic fibrosis [23]. Nws tau raug tshaj tawm tias HA yog cov peev txheej loj ntawm cov txheej txheem extracellular. IV-C raws li lub hauv paus tseem ceeb ntawm lub hauv paus daim nyias nyias yuav ua ke ntau thiab muab tso rau hauv cov theem ua ntej ntawm daim siab cirrhosis. Cov qib ntshav ntawm LN thiab IV-C yog qhov ntsuas ntawm qhov hloov pauv ntawm lub hauv paus membrane thiab qhia txog qib ntawm fibrosis hauv thaj chaw portal thiab sinusoidal capillaries [24]. PC III yog ib qho cim hauv kev kuaj mob ntawm lub siab fibrosis thiab cirrhosis thaum ntxov, tab sis nws qhov rhiab heev thiab qhov tshwj xeeb tsis siab, thiab tsis muaj qhov sib txawv ntawm ntau theem ntawm fibrosis hauv ntau cov ntaub ntawv [24, 25].

Cistanche

Txoj kev tshawb no tau txais txiaj ntsig zoo sib xws. Tsis tas li ntawd, H&E thiab Masson qhov kev soj ntsuam trichrome-stained seem pom pom cov ntaub so ntswg ib txwm muaj nrog cov kab mob siab sib txawv thiab cov kab mob siab sinusoids. Lub siab cov ntaub so ntswg nyob rau hauv cov qauv pab pawg neeg yog disordered, thiab daim siab cov ntaub so ntswg thiab hepatic sinusoid tau hloov los ntawm ib tug loj npaum li cas ntawm cov ntaub so ntswg. Txawm li cas los xij, kev txhim kho tseem ceeb tau pom nyob rau hauv pab pawg kho mob piv nrog rau pawg qauv. Qhov tseem ceeb, collagen hom I thiab collagen hom III qhia ua lub luag haujlwm tseem ceeb hauv kev txhim kho cov kab mob siab fibrosis, qhov thaiv uas tuaj yeem tiv thaiv thiab kho kab mob siab. Yog li ntawd, tiam thiab deposition nyob rau hauv daim siab cov ntaub so ntswg ntawm collagen hom I thiab collagen hom III yuav pab raws li ib tug tseem ceeb determinant ntawm anti-hepatic fibrosis efficiency. TGF- 1 kuj yog ib qho tseem ceeb profibrogenic cytokine nyob rau hauv daim siab raug mob thiab nws yog lom lom nrog ntau yam pharmacological ua [26]. Yuav tsum muaj qhov sib npaug ntawm cov kev ua no los tswj cov ntaub so ntswg homeostasis. Cov lus qhia tsis zoo ntawm TGF- 1 yog koom nrog hauv cov kab mob ntawm daim siab [27, 28]. Nws paub tias TGF- 1 yog ib qho tseem ceeb cytokine uas koom nrog rau theem pib ntawm daim siab fibrosis.


Oxidative stress triggers TGF- 1, ua rau tom kawg stimulating ECM ntau lawm thiab deposition [29]. Yog li, ib qho ntawm cov tswv yim zoo los tsim cov tshuaj tiv thaiv kab mob siab yog txhawm rau txheeb xyuas cov tshuaj tiv thaiv TGF- 1. Immunohistochemical tsom xam pom tias cov kab lus ntawm collagen hom I, collagen hom III thiab TGF- 1 tuaj yeem kuaj xyuas cov txheej txheem pathological ntawm hepatic fibrosis. Cov kev qhia ntawm collagen hom I, collagen hom III thiab TGF- 1 nyob rau hauv cov pab pawg kho mob tau txo qis, uas tau txo qis hauv cov koob tshuaj ntau CPhGs.(phenylethanoid glycoside los ntawm cistanche)pab pawg kho mob tshwj xeeb, thiab qhia tias CPhGs yog ib qho zoo collagen hom I, collagen hom III thiab TGF- 1 inhibitor. Piv txwv li, CPhGs tuaj yeem txhim kho collagenase kev ua haujlwm, tswj kev sib npaug ntawm ECM synthesis thiab degradation, yog li ncua thiab tiv thaiv kev tsim ntawm daim siab fibrosis. CPhGs(phenylethanoid glycoside los ntawm cistanche)Tsis tsuas yog tuaj yeem kho BSA-induced hepatic fibrosis hauv nas tab sis kuj tseem tuaj yeem cuam tshuam nrog inhibiting qhov ua kom HSC hauv vitro. Kev ua kom HSC yog xav tias sawv cev rau theem tseem ceeb ntawm fibrogenesis. Hauv txoj kev tshawb no, cov txiaj ntsig tau piav qhia tias kev tswj hwm ntawm CPhGs los ntawm 25 txog 100 ug / ml zoo kawg nkaus txo qis NF-κB p65, collagen I mRNA qhia, thiab collagen I protein qhia hauv HSC. NF-κB ua lub luag haujlwm tseem ceeb hauv kev hloov kho lub cev tiv thaiv kab mob rau kev kis kab mob lossis stimuli [30]. Lub buildup ntawm NF-κB hauv daim siab hlwb tuaj yeem ua rau kev nrhiav neeg ua haujlwm ntawm cov kab mob cytokines / tus neeg nruab nrab, yog li inducing fibrosis txoj kev loj hlob [31, 32]. Ntxiv mus, collagen kuj yog ib qho rhiab index uas qhia txog qib fibrosis thiab suav txog li 50 feem pua ​​​​ntawm tag nrho cov protein nyob hauv daim siab fibrous [33].

Cistanche extract effects

Raws li qhov tshwm sim, peb tau tshaj tawm tias cov txheej txheem molecular tiv thaiv hepato-fibrosis yog txuas rau CPhGs.(phenylethanoid glycoside los ntawm cistanche)-mediated inactivation ntawm NF-κB qhia, nyob rau hauv uas cov txiaj ntsig pab txhawb kev sib koom ua ke ntawm attenuating immunotoxicity thiab mob kev nyuaj siab hauv BSA-lesioned daim siab cov ntaub so ntswg, ntxiv kho dysmetabolism kom ameliorate daim siab ua haujlwm.

Cov lus xaus

Hauv kev xaus, peb cov kev tshawb fawb qhia tias CPhGs(phenylethanoid glycoside los ntawm cistanche)Nws txo qis qhov cuam tshuam ntawm hepatic fibrosis tshwm sim los ntawm BSA hauv cov nas. Nws cov txheej txheem yuav tsawg kawg yog ib feem yog vim qhov inhibitory nyhuv ntawm CPhGs ntawm cov muaj pes tsawg leeg ntawm ECM thiab stimulation ntawm degradation ntawm ECM, thiab / los yog los ntawm ncaj qha inhibition ntawm lub synthesis ntawm collagen hom I, collagen hom III thiab kev qhia ntawm TGF{ {0}}. Yog li ntawd, peb xav tias CPhGs tuaj yeem siv rau hauv cov khoom kho mob lossis hauv cov tshuaj kho mob rau kev tiv thaiv tib neeg lub siab fibrosis. Cov kev tshawb fawb yav tom ntej yuav tsum tsim kom muaj kev ua tau zoo ntawm CPhGs ua cov tshuaj tiv thaiv kab mob siab fibrosis.

Cov ntawv luv

CPhGs:Phenylethanol glycosides los ntawm cistanche; BSA: Bovine serum albumin; HSC-T6: Hepatic stellate hlwb; Hyp: Hydroxyproline; BJRG: Compound Biejirangan ntsiav tshuaj; TGF- 1: Transforming growth factor 1; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; HA: Hyaluronic acid; LN: Laminin; PC III: Hom III procollagen; IV-C: Hom IV collagen; NF-κB: Nuclear factor kappa-light-chain-enhancer of activated B cells; RT-PCR: Rov qab transcriptase-polymerase saw cov tshuaj tiv thaiv; SDS-PAGE: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Cov kev nyiam sib tw Cov neeg sau ntawv tshaj tawm tias lawv tsis muaj kev sib tw. Cov kws sau ntawv pab txhawb TL, JZ, SPY, LM, MT thiab SLZ tsim thiab tsim cov kev sim. SPY, TL thiab JZ tau txheeb xyuas cov ntaub ntawv. SPY thiab JZ sau cov ntawv sau. TL, LM thiab JZ tau tshuaj xyuas cov ntawv sau. Txhua tus kws sau ntawv tau nyeem thiab pom zoo cov ntawv sau kawg. Kev lees paub Qhov kev tshawb fawb no tau txais kev txhawb nqa los ntawm National Natural Science Foundation ntawm Tuam Tshoj (81260624). Cov kws sau ntawv xav qhia lawv txoj kev ua tsaug rau xibfwb Tao Liu rau nws cov lus pom zoo txhim kho rau kev sau ntawv no. Sau cov ntsiab lus 1 Department of Toxicology, School of Public Health, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi 830011Xinjiang Uyghur Autonomous


Thaj tsam, Suav teb. 2 Lub Chaw Haujlwm Tseem Ceeb rau Uighur Tshuaj, Lub Tsev Haujlwm ntawm Materia Medica ntawm Xinjiang, Urumqi 830004, Suav. 3 No. 140 Xinhua South Road, Tianshan District, Urumqi 830000Xinjiang Uyghur Autonomous Region, Suav. Tau Txais: 6 Lub Yim Hli 2015 Txais: 24 Kaum Ib Hlis 2015

Cistanches effects

Cov ntaub ntawv

  1. Cohen-Naftaly M, Friedman SL. Cov xwm txheej tam sim no ntawm kev kho mob antifibrotic tshiab hauv cov neeg mob uas muaj kab mob siab ntev. Adv Gastroenterol Cov. 2011; 4(6):391–417.

  2. Puche JE, Saiman Y, Friedman SL. Hepatic stellate cells thiab daim siab fibrosis. Lub cev muaj zog. 2013; 3(4): 1473–92.

  3. Hernandez-Gea V, Friedman SL. Pathogenesis ntawm daim siab fibrosis. Annu Rev Pathol. 2011; 6:425–56.

  4. Sohrabpour AA, Mohamadnejad M, Malekzadeh R. Review article: the reversibility of cirrhosis. Aliment Pharmacol Ther. 2012; 36(9):824–32.

  5. Raven PH, Zhang LB, Ventenat O. Suav academy of sciences. 23 ed ib. Tuam Tshoj: Editorial Committee ntawm Flora ntawm Tuam Tshoj; 2013. p. 1–16.

  6. Suav Pharmacopoeia Commission ntawm Sanitary Ministry of the People's Republic of China. Suav pharmacopoeia, Part 1. Tuam Tshoj: Tshuaj Kev Lag Luam Publishing House; 2010. p. 126.

  7. Yan GH, Tian JH, Long BW, Li N. Kev tshawb fawb nce qib ntawm phenylethanoid glycosides los ntawm Cistanche tubulosa. Central South Pharm. 2012; 10:692–5.

  8. Li J, Huang D, He L. Effect of Rou Cong Rong(Herba Cistanches Deserticola) ntawm kev ua me nyuam toxicity nyob rau hauv nas induced los ntawm glycoside ntawm Leigongteng (Radix et Rhizoma Tripterygii). J Tradit Chin Med. 2014; 34(3):324–32.

  9. Xing Y, Liao J, Tang Y, Zhang P, Tan C, Ni H, et al. ACE, thiab platelet aggregation inhibitors los ntawm Tamarix Hojnacki Bunge (tus tswv cog ntawm Herba Cistanches) loj hlob hauv Xinjiang. Pharmacogn Mag. 2014; 10(38): 111–7.

  10. Jia Y, Guan Q, Jiang Y, Salh B, Guo Y, Tu P, et al. Amelioration ntawm dextran sulfate sodium-induced colitis hauv nas los ntawm echinacoside-enriched extract ntawm Cistanche tubulosa. Phytother Res. 2014; 28(1):110–9.


Koj Tseem Yuav Zoo Li