Ntu 1: Cov Ncauj Ncauj Ncauj Ncauj Qhia Tsis Txaus Ntshai Kev Hloov Kho hauv Phau Tshiab OUL Hloov Kho Vaj Tse

Yog xav paub ntxiv:Ali.ma@wecistanche.com

Pls nyem qhov no mus rau Ntu 2

Janine L. Kwapis1,2, Yasaman Alaghband1, Ashley A. Keiser1, Tri N. Dong1, Christina M. Michael1, Diane Rhee1, Guanhua Shu1, Richard T. Dang1, Dina P. Matheos1, thiab Marcelo A. Wood1

Kev nco tsis nyob mus ib txhis, lub xeev zoo li qub tab sis yuav tsum tau hloov pauv hloov pauv hauv cov lus teb rau cov ntaub ntawv tshiab. Txawm tias tshiabncokev tsim yog feem ntau kawm nyob rau hauv lub chaw soj nstuam, feem ntau cov koom haum tiag tiag hauv ntiaj teb yog kev hloov kho rau cov kev nco uas twb muaj lawm, tshwj xeeb tshaj yog nyob rau hauv cov laus, kev paub txog lub hlwb. Txog rau tam sim no, daim teb tau tsis muaj kev coj tus cwj pwm yooj yim uas tuaj yeem ntsuas seb cov ntaub ntawv qub thiab hloov tshiab puas nco qab hauv ib qho kev sim ib zaug. Txhawm rau daws qhov sib txawv no, peb tau tsim ib qho tshiabncoKev hloov kho lub ntsiab lus, hu ua Cov Khoom Siv Hauv Qhov Chaw Kho Tshiab (OUL) txoj haujlwm uas muaj peev xwm ntsuasncohloov kho nyob rau hauv ib txoj hauj lwm uas tsis muaj kev ntxhov siab uas tsim nyog rau cov nas me thiab laus. Peb thawj zaug qhia tias cov nas me ua tiav nco ntsoov ob qho tib si lub cim xeeb thiab cov ntaub ntawv tshiab hauv OUL. Tom ntej no, peb ua kom pom tias intrahippocampal infusion ntawm cov protein synthesis inhibitor anisomycin cuam tshuam ob qho tib si cov ntaub ntawv tshiab thiab lub cim xeeb qub ntawm kev sim, qhia tias kev hloov kho nco hauv OUL koom nrog lub cim xeeb qub. Txhawm rau txheeb xyuas qhov no, peb tau siv cov txheej txheem Arc CatFISH los qhia tias OUL kev hloov kho tshiab tau rov ua haujlwm ntau dhau ntawm cov neurons raws li qhov qub.nco. Thaum kawg, siv OUL, peb qhia tiasncokev hloov kho yog qhov tsis zoo hauv kev laus, 18-mo nas. Ua ke, cov txiaj ntsig no qhia tau tias hippocampalncoKev hloov kho tshiab yog cuam tshuam nrog kev laus thiab tsim kom muaj OUL paradigm yog ib txoj hauv kev zoo, rhiab heev ntawm kev ntsuam xyuas lub cim xeeb hloov kho hauv nas.

Nyem rauCistanche hauv Urdu txhawm rau txhim kho kev nco

Taw qhia

Cov cim xeeb yuav tsum tau hloov kho tsis tu ncua kom suav nrog cov ntaub ntawv tseem ceeb tshaj plaws, tsis ntev los no rau hauv kev khaws cia. Qhov no muaj peev xwm los ua ke cov ntaub ntawv tshiab rau hauv uas twb muaj lawmncoNws yog ib qho tseem ceeb kom tso cai rau cov kab mob los cia siab tias yuav tshwm sim yav tom ntej thiab hloov mus rau qhov xwm txheej tshiab. Feem ntau cov kev nco tsis yog cov koom haum tshiab tab sis yog kev hloov pauv lossis ntxiv (hloov tshiab) rau cov kev nco uas twb muaj lawm, tshwj xeeb tshaj yog nyob rau hauv cov laus, kev paub txog lub hlwb. Txawm hais tias nws yog qhov tseem ceeb, cov txheej txheem uas txhawb kev hloov kho lub cim xeeb yog qhov tsis zoo, thiab txawm tias tsawg dua yog to taub txog qhov tsis zoo ntawm cov txheej txheem no yuav ua rau muaj hnub nyoog txog kev txawj ntse poob. Kev nkag siab tias yuav hloov pauv li cas hauv cov lus teb rau cov ntaub ntawv tshiab, yog li ntawd, ib kauj ruam tseem ceeb rau kev txhim khoncohla lub neej.

Kev nco tuaj yeem hloov kho los ntawm cov txheej txheem hu ua "reconsolidation," nyob rau hauv uas retrieval ua rau lub sij hawm ntawm lability uas yuav tso cai rau cov ntaub ntawv tshiab muab tso rau hauv ib qho uas twb muaj lawm, ruaj khov.nco. Cov txheej txheem reconsolidation muaj cov theem pib destabilization cim los ntawm cov protein degradation tom qab los ntawm ib theem restabilization yam ntxwv los ntawm protein synthesis [1-9]. Cov hauj lwm tsis ntev los no tau ua pov thawj tias kev sib koom ua ke tsuas yog pib thaum cov ntaub ntawv tshiab nthuav tawm thaum rov muab dua; thaum retrieval muaj tsuas paub cov ntaub ntawv, tus thawjnconyob ruaj khov thiab tiv taus cov tshuaj amnesic xws li protein synthesis inhibitors [2, 10–16]. Qhov no qhia tias cov ntaub ntawv tshiab ua rau lub cim xeeb tsis ruaj khov kom tso cai rau kev hloov kho. Raws li qhov no, ntau cov kev tshawb fawb tam sim no qhia tau hais tias cov ntaub ntawv tshiab tau nthuav tawm thaum rov qab tuaj yeem hloov cov ntsiab lus ntawm kev nco [2, 3, 11, 17, 18], qhov cuam tshuam ntawm kev nco [19–22], ua rau muaj kev ploj tuag tsis tu ncua [23–27. ], lossis tseem rov txhim kho lub cim xeeb ntawm qib Circuit Court [11, 28].

qhov chawncotxoj hauj lwm, hu ua Cov Khoom hauv Qhov Chaw Hloov Kho Tshiab (OUL) paradigm. Lub hom phiaj ntawm txoj kev tshawb no yog ob npaug. Ua ntej, peb tsom los tsim txoj haujlwm OUL raws li txoj hauv kev tshiab rau kev kawm hippocampus-dependentncohloov kho. Qhov thib ob, peb tsom los siv lub tswv yim no los txiav txim seb puas yogncokev hloov kho yog qhov tsis zoo nrog lub hnub nyoog.

OUL paradigm yog tshiab nyob rau hauv uas nws muaj peev xwm ntsuam xyuas ob qho tib si lub cim xeeb thiab cov ntaub ntawv tshiab nyob rau hauv ib qho kev sim ib zaug. Tsis tas li ntawd, OUL siv qhov xwm txheej kev kawm uas siv qhov zoo ntawm cov nas 'innate nyiam rau qhov tshiab, zam kev ntxhov siab tsis tsim nyog thiab ua rau nws tsim nyog rau kev ntsuas hnub nyoog ntsig txog qhov tsis zoo hauv kev hloov kho nco. Ntawm no, peb thawj zaug lees paub txoj haujlwm OUL, ua qauv qhia tias cov nas hluasncorau ob qho tib si thawj qhov kev cob qhia thiab kev nco hloov tshiab hauv OUL kev xeem sib ntsib. Tom ntej no, peb tau siv ob txoj hauv kev ntxiv (intra-hippocampal anisomycin txhaj tshuaj thiab Arc CatFISH) los xyuas kom meej tias OUL hloov tshiab yuav tsum tau rov qab los ntawm lub cim xeeb qub, tsis yog tsim lub cim xeeb tshiab, ywj siab. Thaum kawg, siv OUL, peb pom tau tias cov nas laus pom qhov tsis zoo hauvncokev hloov kho tshiab, qhia tias qhov tsis muaj peev xwm hloov kho kev nco yuav ua rau muaj hnub nyoog txog kev paub txog kev poob qis. Ua ke, cov txiaj ntsig no qhia tau tias OUL paradigm tuaj yeem siv los nkag siab txog cov txheej txheem hauv qab hippocampal nco kev hloov kho thoob plaws lub neej.

Cov khoom siv thiab cov txheej txheem

Cov nas

Cov kev kawm yog cov neeg laus (2–4-hli) lossis hnub nyoog (18–20 lub hlis) txiv neej C57BL/6 J nas (Jackson Laboratory). Cov nas tau nyob hauv tsev, noj, thiab ua haujlwm raws li tau piav qhia hauv Cov Txheej Txheem Ntxiv. Tag nrho cov txheej txheem tau pom zoo los ntawm University of California, Irvine's Institutional Animal Care and Use Committee thiab tau ua raws li National Institutes of Health cov lus qhia.

Peb txoj hauj lwm

Tom qab tuav thiab nyob ib puag ncig (saib Cov Txheej Txheem Ntxiv), cov nas tau raug cob qhia nrog ob yam khoom zoo ib yam hauv qhov chaw tshwj xeeb (A1 thiab A2) rau 1 lossis 3d hauv cov ntsiab lus nyob. Nees nkaum plaub teev tom qab ntawd, cov nas tau muab qhov kev hloov kho tshiab, uas lawv tau raug xa mus rau qhov Tsis Muaj Hloov Kho lossis Hloov Kho. Tsis muaj Update nas tau rov raug rau qhov chaw kawm A1 thiab A2. Rau Hloov tshiab nas, ib yam khoom raug tsiv mus rau qhov chaw tshiab (A3). Thaum kawg, cov nas tau muab qhov kev sim khaws cia uas lawv tau nthuav tawm rau peb yam khoom hauv qhov chaw yav dhau los (A1, A2, A3) thiab cov khoom thib plaub hauv qhov chaw tshiab A4.Ncorau qhov kev cob qhia thawj tsim tau txiav txim siab los ntawm kev sib piv kev tshawb nrhiav qhov chaw tshiab A4 rau qhov chaw A1 thiab A2.Ncorau qhov hloov tshiab tau txiav txim siab los ntawm kev sib piv tshawb nrhiav qhov chaw tshiab A4 rau qhov chaw A3.

Cannulation phais

Cov nas tsuag hauv daim duab 2 tau cog nrog cov kab mob ntev ntev raws li tau piav qhia yav dhau los [37] kom tso cai rau hippocampal infusion ncaj qha ntawm anisomycin tom qab hloov kho (saib Cov Txheej Txheem Ntxiv). Cov nas rov zoo li tsawg kawg yog 7d ua ntej pib kuaj tus cwj pwm. Tam sim ntawd tom qab qhov kev hloov kho tshiab, cov nas tau muab ob sab nrog anisomycin (ANI, 125/ug/μL) lossis tsheb (VEH) rau hauv dorsal hippocampus (1.0 μL/sab). Cannula qhov chaw tau lees paub los ntawm staining coronal slices nrog cresyl violet.

CatFISH

Fluorescence hauv situ hybridization rau CatFISH tau ua raws li tau piav qhia yav dhau los [38, 39] (saib Ntxiv rau cov ntsiab lus). Lub DIG-labeled Arc antisense riboprobe tau hybridized nrog cov ntaub so ntswg thaum hmo ntuj thiab pom nrog ib qho anti-DIG-HRP conjugate, pom nrog cov khoom siv Cy3 substrate, thiab counterstained nrog DAPI.

Confocal dluab tau sau nyob rau hauv cheeb tsam CA1b ntawm dorsal hippocampus thiab cov dluab tau qhab nia los txheeb xyuas cov subcompartment localization ntawm Arc los xam cov qhab nia zoo sib xws li yav tas los tau piav qhia [39].

Kev txheeb cais

OUL txoj haujlwm tau muab qhab-nees los ntsuas lub sijhawm tshawb nrhiav khoom raws li tau piav qhia yav dhau los rau qhov chaw nyobncokev sim [40, 41]. Kev txheeb xyuas txheeb cais (saib Ntxiv) tau ua tiav siv ob-tailed Student's t-tests, one-way ANOVAs, or two-way ANOVAs with Sidak-corrected t-tests. Mixed-qauv ANOVAs tau siv thaum ib qho kev sib txawv yog qhov rov ntsuas dua. Tus nqi ntawm 0.05 yog xav tau rau qhov tseem ceeb.

TSEEM CEEB

Cov menyuam yaus ua yeeb yam tau zooncorau ob qho tib si kev cob qhia thiab hloov kho cov ntaub ntawv hauv OUL

Peb thawj zaug tsom mus rau kev lees paub OUL txoj haujlwm uas muaj peev xwm ntsuas tauncohloov kho cov nas me (Fig. 1a). Tom qab kev nyob ib puag ncig, nas tau raug cob qhia thawj zaug kom paub qhov chaw ntawm ob yam khoom zoo ib yam hauv cov ntsiab lus uas paub (kev cob qhia, Hnub 1). Hnub tom qab, thaum lub sijhawm hloov tshiab (Hnub 2), nas tau muab faib ua ob pawg. Pab pawg Tsis Muaj Hloov Kho tau rov nthuav tawm rau ob qhov chaw paub (A1 thiab A2). Pab pawg hloov tshiab tau nthuav tawm rau ib qho chaw paub (A1) thiab ib qho khoom txav mus rau qhov chaw tshiab (A3). Tag nrho cov pab pawg tau muab qhov kev sim (Hnub 3) txhawm rau ntsuas cov tsiaj lub cim xeeb rau ob qho tib si thawj qhov chaw thiab qhov chaw tshiab. Ua pov thawj, nas tau raug rau plaub yam khoom zoo ib yam: peb nyob rau hauv qhov chaw uas tau nthuav tawm yav dhau los (A1, A2, thiab A3) thiab ib qho hauv qhov chaw tshiab (A4).Ncorau qhov kev cob qhia thawj zaug tau soj ntsuam los ntawm kev sib piv kev tshawb nrhiav qhov chaw tshiab cov khoom (A4) mus rau kev tshawb fawb ntawm qhov chaw kawm (A1 thiab A2).Ncorau cov ntaub ntawv kho tshiab tau raug soj ntsuam los ntawm kev sib piv kev tshawb fawb ntawm qhov chaw tshiab khoom (A4) mus rau kev tshawb nrhiav qhov "hloov tshiab" qhov chaw (A3). Raws li cov nas nyiam qhov tshiab, nco txog qhov kev cob qhia thawj zaug lossis cov ntaub ntawv tshiab tau pom los ntawm kev tshawb nrhiav ntau ntxiv ntawm cov khoom hauv qhov chaw tshiab (A4) piv rau txhua yam ntawm peb yam khoom (qhia los ntawm cov qhab nia siab dua ntawm qhov ntsuas kev ntxub ntxaug ( DI), saib cov txheej txheem).

Thaum lub sij hawm kev cob qhia, nas nyob rau hauv ob pab pawg tau pom zoo sib xws ntawm kev tshawb fawb ntawm cov khoom A1 thiab A2, ua rau muaj kev ntxub ntxaug index (DI) zero rau ob pawg (Fig. 1bi; ob-tailed Student's t-test: t(17) {{ 6}}.086, p=0.40). Tag nrho cov qib tshawb nrhiav kuj zoo ib yam ntawm Cov Pab Pawg Tsis Hloov Kho thiab Hloov Kho thaum lub sijhawm kawm (Fig. 1bii; ob-tailed Student's t-test: t(17)=0.035, p=0.97, n=10,9).

Kom paub meej tias qhov khoom qub qhov chawncotau txais kev vam meej, peb tau soj ntsuam kev ua tau zoo tom ntej thaum lub sijhawm hloov tshiab (Fig. 1c). Cov nas nyob rau hauv pab pawg Tsis Hloov Kho tau pom tias muaj kev nyiam sib npaug rau qhov chaw rov nthuav tawm A1 thiab A2, ua rau DI zero zero (Daim duab 1ci). Cov nas nyob rau hauv pab pawg Hloov tshiab, ntawm qhov tod tes, nyiam tshawb nrhiav cov khoom txav A3 piv rau cov khoom tsis tau txav mus los A1 thiab pom qhov tseem ceeb DI siab dua piv rau pawg Tsis Hloov Kho (Fig. 1ci, ob-tailed Student's t-test: t( 17)=3.57, p=0.002). Kev tshawb nrhiav tag nrho kuj tseem ceeb dua hauv pab pawg Hloov tshiab piv rau Pab Pawg Tsis Muaj Hloov Kho thaum lub sijhawm hloov kho tshiab (Daim duab 1cii; ob-tailed Student's t-test: t(17)=3.39, p {{22} }.004), qhia tias nas siv sijhawm ntau dua los tshawb txog cov khoom thaum qhov chaw tshiab tau qhia. Ua ke, cov txiaj ntsig no tau lees paub tias qhov chaw ntawm cov khoom qub tau kawm hauv cov nas me uas raug rau 10- ntu kev cob qhia min, raws li cov ntaub ntawv dhau los [41, 42].

Los txiav txim seb tus thawjncotau hloov kho kom suav nrog qhov chaw kho tshiab (A3), cov nas tau muab qhov kev sim uas txhua qhov chaw paub (A1, A2, thiab A3) tau simtawm tsam qhov chaw tshiab (A4) (Fig. 1d). Raw feem pua ​​​​ntawm lub sij hawm tshawb nrhiav rau txhua yam ntawm plaub yam khoom thaum lub sij hawm sim kev sib kho yog qhia nyob rau hauv Ntxiv Fig. 5A. Ob pab pawg tau pom zoo lawmncorau cov ntaub ntawv tseem ceeb, raws li ob qho tib si Hloov Kho thiab Tsis Hloov Kho tsiaj zoo ib yam li qhov chaw tshiab A4 tshaj ob qhov chaw qub A1 (Daim duab 1di; ob-tailed Tub Kawm Ntawv's t-test: t(17)=0.31, p { {8}}.76) thiab A2 (Fig. 1dii; ob-tailed Student's t-test: t(17)=1.66, p=0.12). Qhov no lees paub tias tom qab hloov kho tshiab, cov ntaub ntawv qub tseem khaws cia. Txhawm rau kuaj seb cov ntaub ntawv kho tshiab puas tau kawm tiav, peb kuj tau muab piv rau kev tshawb nrhiav qhov chaw tshiab A4 rau qhov chaw tshiab A3 thaum lub sijhawm xeem. Cov nas nyob rau hauv pab pawg Tsis Hloov Kho tau pom tias muaj kev nyiam sib npaug rau cov khoom A3 thiab A4, ua rau DI uas tsis muaj qhov txawv txav ntawm xoom (Daim duab 1diii; ib qho piv txwv t-test piv rau 0: t(9)=1.55, p {{30}}.156). Cov nas muab qhov hloov tshiab, ntawm qhov tod tes, nyiam tshawb nrhiav qhov chaw tshiab A4 dhau qhov chaw tshiab A3 (Daim duab 1diii, ib qho qauv t-test piv rau 0: t(8)=3.42, p { {41}}.009; ob-tailed Student's t-test comparing Update to No Update: t(17)=2.92, p=0.0096), qhia tias lawv nco qab qhov chaw tshiab. Tsis muaj qhov sib txawv ntawm tag nrho cov kev tshawb fawb thaum lub sij hawm sim kev sib ntsib ntawm pawg (Fig. 1div; ob-tailed Student's t-test: t(17)=1.27, p=0.22). Ua ke, cov txiaj ntsig no tau lees paub tias cov tsiaj hluas pom cov cwj pwm nyiam ua raws li kev rov qab ua tiav ntawm ob qho tib si thawj cov ntaub ntawv thiab cov ntaub ntawv tshiab ntawm kev sim.

Fig. 1 Cov nas me ua tau zooncohloov kho hauv OUL txoj haujlwm. kev sim tsim. b Kev cob qhia kev coj cwj pwm. Cov nas qhia DI qis, qhia tias tsis nyiam rau cov khoom A1 lossis A2 (sab laug), thiab muaj cov qib zoo sib xws ntawm tag nrho cov khoom tshawb nrhiav (sab xis). c Hloov tshiab kev coj cwj pwm. (i) Cov nas nyob rau hauv cov xwm txheej hloov tshiab nyiam qhov chaw tshiab A3 mus rau qhov chaw paub A1 whereas Tsis Hloov Kho nas qhia zoo ib yam kev tshawb nrhiav ntawm qhov chaw paub A1 thiab A2, nrog DI zero. (ii) Hloov cov nas qhia qhov tseem ceeb tshaj qhov kev tshawb nrhiav tag nrho ntau dua li Tsis Muaj Hloov Kho nas. d Kev coj cwj pwm thaum lub sij hawm xeem. (i) Cov nas nyob rau hauv ob qhov Hloov Kho thiab Tsis Muaj Hloov Kho Pab Pawg qhia tsis nco qab rau qhov chaw kawm thawj qhov chaw A1. (ii) Ob pawg kuj qhiancorau qhov chaw kawm thawj A2. (iii) Tsuas yog pab pawg hloov tshiab qhia qhov nyiam rau qhov chaw tshiab A4 dhau qhov chaw tshiab A3; Tsis hloov tshiab nas nyiam cov khoom A3 thiab A4 sib npaug. (iv) Cov nas pom zoo sib xws ntawm tag nrho cov khoom tshawb nrhiav thaum lub sijhawm sim. Cov ntaub ntawv raug nthuav tawm raws li txhais tau tias ± SEM. **p <>

Tom qab hloov kho hippocampal protein synthesis inhibition cuam tshuam ob qho tib si cov ntaub ntawv tshiab thiab cov thawjncoPeb tom ntej no sim seb cov ntaub ntawv kho tshiab uas tau kawm hauv OUL txoj haujlwm puas hloov kho qhov qubncolos yog tsim ib lub koom haum tshiab, ywj siab. Rau txoj haujlwm OUL los ua tus qauv muaj txiaj ntsig ntawm kev hloov kho lub cim xeeb, thawj lub cim xeeb yuav tsum tau muab rov qab thiab hloov kho kom suav nrog cov khoom tshiab cov ntaub ntawv qhov chaw nthuav tawm thaum lub sijhawm hloov tshiab. Txhawm rau kuaj qhov no, peb siv ob txoj hauv kev ntxiv los tshuaj xyuas seb qhov qubncoyog koom nrog OUL kev hloov kho tshiab: anisomycin infusions thiab catFISH tsom xam.

Ua ntej, peb tau sim seb puas yog anisomycin thov tom qab qhov kev hloov kho tshiab yuav cuam tshuamncorau cov ntaub ntawv kev cob qhia thawj. Ntau cov kev tshawb fawb tau pom tias tom qab muab cov tshuaj tua kab mob ntawm cov protein synthesis inhibitor anisomycin (ANI) tuaj yeem thaiv kev sib koom ua ke hauv qee yam mob [43, 44] tsawg kawg ib ntus [saib 45]. Nco ntsoov, tus thawjncoyog ua labile los ntawm cov ntaub ntawv tshiab uas tau nthuav tawm thaum lub sij hawm retrieval tab sis tsis ua kom zoo nyob rau hauv qhov tsis muaj de novo protein synthesis, ua rau muaj kev cuam tshuam ntawm lub cim xeeb qub [6-9]. Thaum tsis muaj cov ntaub ntawv tshiab tau nthuav tawm thaum lub sijhawm rov mus sim, ntawm qhov tod tes, ANI infusions tsis muaj txiaj ntsig, vim cov ntaub ntawv tshiab yuav tsum tau pib rov ua dua [6].

Txhawm rau txiav txim siab seb cov ntaub ntawv hloov kho tshiab hauv OUL txoj haujlwm puas cuam tshuam tus thawjnco, peb infused anisomycin tam sim ntawd tom qab qhov kev hloov kho tshiab thiab ntsuam xyuas seb qhov no puas ua rau lub koom haum qub (Fig. 2a). Peb infused ANI ncaj qha rau hauv dorsal hippocampus (DH) tom qab qhov kev hloov kho tshiab (Daim duab 2b, ib tus neeg cannula qhov chaw qhia nyob rau hauv Ntxiv Fig. 1A), raws li spatial khoom qhov chaw nco yog tshwj xeeb yog rhiab heev rau manipulations hauv cheeb tsam no [40, 46, 47 ] thiab qhov no yuav tso cai rau peb mus localize lubncohloov tshiab thiab zam cov teebmeem tawm ntawm lub ntiaj teb cov protein synthesis inhibition. Cov nas tau pom zoo li kev tshawb nrhiav cov khoom A1 thiab A2 ntawm kev cob qhia (ob txoj kev ANOVA, tsis muaj qhov cuam tshuam loj lossis hloov kho x tshuaj sib cuam tshuam) thiab muaj qhov zoo sib xws ntawm kev tshawb nrhiav tag nrho (ob txoj kev ANOVA, tsis muaj qhov cuam tshuam loj lossis hloov kho x tshuaj sib cuam tshuam) (Ntxiv daim duab 1B, C). Thaum lub sijhawm hloov tshiab, cov nas nyob rau hauv qhov Kev Hloov Kho tshiab tau pom qhov tseem ceeb nyiam rau cov khoom txav, thiab cov nas nyob rau hauv qhov tsis hloov tshiab qhov xwm txheej txuas ntxiv qhia qhov kev tshawb fawb zoo sib xws ntawm cov khoom hauv ob qhov chaw paub (Fig. 2ci; ob txoj kev ANOVA, qhov tseem ceeb tseem ceeb. cuam ​​tshuam ntawm kev hloov tshiab (F(1,29) {{10}}.31, p < 0.0001),="" tab="" sis="" tsis="" muaj="" qhov="" cuam="" tshuam="" loj="" ntawm="" kev="" siv="" tshuaj="" lossis="" kev="" cuam="" tshuam="" tsis="" zoo,="" n="6,6,12,9" )).="" tsis="" muaj="" pab="" pawg="" sib="" txawv="" ntawm="" cov="" tsiaj="" uas="" tau="" txais="" anisomycin="" lossis="" tsheb="" thiab="" tsis="" muaj="" qhov="" sib="" txawv="" ntawm="" tag="" nrho="" cov="" khoom="" tshawb="" nrhiav="" tau="" pom="" thaum="" lub="" sijhawm="" hloov="" tshiab="" (daim="" duab="" 2cii;="" ob="" txoj="" kev="" anova,="" tsis="" muaj="" kev="" cuam="" tshuam="" loj="" lossis="" kev="" cuam="" tshuam).="" qhov="" no="" qhia="" tias="" ua="" ntej="" anisomycin="" infusion,="">

Fig. 2 Kev cuam tshuam cov protein synthesis tom qabncohloov kho impairs ob tus thawjncothiab cov ntaub ntawv tshiab hauv cov nas me. kev sim tsim. b Tus neeg sawv cev cannula qhov chaw qhov chaw tsom mus rau CA1 ntawm dorsal hippocampus. c Hloov tshiab kev coj cwj pwm. (i) Ob pawg nas nyob rau hauv qhov kev hloov kho tshiab nyiam qhov chaw tshiab A3 dhau qhov chaw paub A1 thaum Tsis Muaj Hloov Kho nas qhia DI zero, qhia tias tsis nyiam. (ii) Cov nas pom zoo ib yam li kev tshawb nrhiav thoob plaws hauv pab pawg. d Kev coj cwj pwm thaum lub sij hawm xeem. (i) Rau thawj qhov kev cob qhia A1, cov nas nyob rau hauv qhov tsis muaj qhov hloov tshiab qhia tau tias tsis nco qab tsis hais txog kev kho tshuaj. Rau cov xwm txheej hloov tshiab, anisomycin-kho nas qhia pom qhov txo qis DI rau A1 piv rau kev tswj tsheb, qhia tias anisomycin tsis zoo.ncorau cov ntaub ntawv qhia thawj zaug hauv Update tsiaj. (ii) Rau thawj qhov kev cob qhia A2, tsis muaj qhov cuam tshuam tseem ceeb ntawm anisomycin tau pom nyob rau hauv No Update lossis Update tsiaj. (iii) Rau qhov chaw kho tshiab A3, Tsis Muaj Cov Tsiaj Hloov Kho Tsiaj pom muaj DI qis dua piv rau cov khoom A4, tsis hais txog tshuaj infusion. Hloov kho cov tsiaj txhu muab anisomycin pom qhov tseem ceeb DI qis dua li kev tswj hwm tsheb, qhia tias anisomycin ua rau tsis nco qab rau cov ntaub ntawv kho tshiab. iv) Tsis muaj qhov sib txawv tseem ceeb hauv kev tshawb nrhiav tag nrho ntawm qhov kev sim. Cov ntaub ntawv raug nthuav tawm raws li txhais tau tias ± SEM. **p < 0.01,="" ***p="">< 0.001,="" ****p="">< 0.0001.="" veh,="" tsheb;="" ani,="">