Kev ua kom zoo ntawm cov cawv txiv hmab-soaki thiab ci ua cov txheej txheem thiab cov qauv zoo rau cistanche fortrancho
Feb 27, 2025
2.8 kev tshawb nrhiav zoo
2.8.1 Cov Cim Cim
Tom qab ua19 batches ntawm cistanche Showicola Decoction daimSiv cov qauv zoocawv-soaking thiab txoj kev ci, lawv cov yam ntxwv tau piav raws li nram no:
Lawv tshwm sim liIrregular Tuab Tuabnrog atsaus xim av lossis daj daj-xim avCov. Cov ntoo khaub lig-seem pomdot-zoo li vascular bundles npaj hauv cov kab sib twCov. Cov kev ntxhib los mos yog qhov nyuaj, nrog me ntsisCawv Aroma, a Qab zib saj, thiab aTsawg me ntsis ntsimCov. Sawv cev kuaj(S1–S3)yog qhia hauvDaim Duab 2.
Daim duab 2:Cov qauv ntawm peb lub catches ntawm cawv-soaked thiab ciCistancho verticola decoction daim.
Cistanche extract nrog 16% ua yeeb yam
Nyem rau cov lus qhia ntxiv
2.8.2 TSHUAJ
Ua raws li cov hau kev teev hauv2020 tsab ntawm cov kws muag khoom Suav, noo noo cov ntsiab lus thiab tag nrho cov hmoov tshauv yog ntsuasnyob rau hauv triplicate, thiab cov txiaj ntsig tau nthuav tawm hauvRooj 12.
TusYauv cov ntsiab lusntawm 19 batches ntawmCawv-soaked thiab ci cistanche Showicola Decoction daimranged los ntawm5.56% rau 9.14%.
TusTag nrho cov hmoov tshauvranged los ntawm4.64% rau 7.63%.
Vim muajHloov pauv nyob rau hauv decoction thooj zoo, tustxhais tau tias qhov tseem ceebntawm cov ntsiab lus noo noo thiab tag nrho cov ntoo tshauvnce los ntawm 30%los tsim cov qauv hauv qab no:
Cov ntsiab lus ya raws yuav tsum tsis pub tshaj 9.56%.
Tag nrho cov hmoov tshauv yuav tsum tsis pub tshaj 8.15%.
2.8.3 Kev txiav txim siab ntawm cov ntsiab lus tsis muaj teeb meem thiab cov ntsiab lus sib xyaw
Ua raws li tus2020 tsab ntawm cov kws muag khoom Suav, cov ntsiab lus ntawmmuaj teeb meem extractabletau txiav txim siab. Cov ntsiab lus ntawmCheebtsamraug ntsuas raws li txoj kev piav qhia hauvTshooj 2.1, nrog cov txiaj ntsig tau qhia hauvRooj 12.
TusCov ntsiab lus muaj teeb meem cov ntsiab lusranged los ntawm57.38% rau 71.91%, nrog ibTus nqi nruab nrab ntawm 66.48%.
Tag nrho cov ntsiab lus ntawmEchinacoside thiab Verbualcosideranged los ntawm{{0 {}}. 37% mus rau 1.52%.
Vim muajHloov pauv nyob rau hauv decoction thooj zoo, tusQhov tseem ceeb nruab nrab tau txo los ntawm 30%los tsim cov qauv hauv qab no:
Cov ntsiab lus teeb meem yuav tsum tsis pub tsawg tshaj 46.54%.
Tag nrho cov ntsiab lus ntawm Echinacoside thiab Verbascoside yuav tsum tsis txhob tsawg tshaj li 0. 59%.
Daim duab 2 Cov qauv ntawm peb kab ntawm cov cawv-soaked roasted cistancho squaticola slices
Cov Lus 12: Kev Txiav Txim Siab ntawm cov ntsiab lus noo, tag nrho cov ntoo tshauv, rho cov ntsiab lus, thiab siv cov dej cawv hauv 19 soaked thiab ciContancheDecoction daim (%)
| Khoom sim | Noo noo (%) | Tag Nrho Cov Tshauv (%) | Extract (%) | Echinacoside (%) | Cistanoside A (%) | Tubuloside A (%) | Verbaside (%) | Is theoverebastroside (%) | 2'-acetylverboscoside (%) |
|---|---|---|---|---|---|---|---|---|---|
| S1 | 8.43 | 6.56 | 70.39 | 0.96 | 0.20 | 0.01 | 0.27 | 0.06 | 0.27 |
| S2 | 7.23 | 7.28 | 69.17 | 0.90 | 0.20 | 0.03 | 0.23 | 0.06 | 0.05 |
| S3 | 8.02 | 7.26 | 70.09 | 1.22 | 0.23 | 0.03 | 0.30 | 0.06 | 0.02 |
| S4 | 8.40 | 6.66 | 70.73 | 0.78 | 0.23 | 0.05 | 0.28 | 0.04 | 0.12 |
| S5 | 8.01 | 7.63 | 68.73 | 0.81 | 0.23 | 0.03 | 0.31 | 0.06 | 0.21 |
| S6 | 9.14 | 7.42 | 66.09 | 0.19 | 0.11 | 0.01 | 0.18 | 0.03 | 0.18 |
| S7 | 8.77 | 6.57 | 63.14 | 0.39 | 0.16 | 0.01 | 0.17 | 0.03 | 0.14 |
| S8 | 7.07 | 6.60 | 65.56 | 0.30 | 0.16 | 0.04 | 0.09 | 0.02 | 0.13 |
| S9 | 8.32 | 5.33 | 63.16 | 0.57 | 0.24 | 0.02 | 0.21 | 0.04 | 0.09 |
| S10 | 6.18 | 6.93 | 64.51 | 0.41 | 0.10 | 0.03 | 0.29 | 0.04 | 0.10 |
| S11 | 8.60 | 6.68 | 68.47 | 0.60 | 0.23 | 0.03 | 0.34 | 0.05 | 0.15 |
| S12 | 7.58 | 6.15 | 69.24 | 1.18 | 0.16 | 0.04 | 0.37 | 0.05 | 0.12 |
| S13 | 6.32 | 4.64 | 69.89 | 0.79 | 0.16 | 0.04 | 0.19 | 0.04 | 0.02 |
| S14 | 5.56 | 4.83 | 62.95 | 0.56 | 0.07 | 0.03 | 0.08 | 0.04 | 0.02 |
| S15 | 6.67 | 7.36 | 62.81 | 0.92 | 0.10 | 0.02 | 0.20 | 0.10 | 0.02 |
| S16 | 6.49 | 6.49 | 71.91 | 0.36 | 0.08 | 0.02 | 0.10 | 0.05 | 0.02 |
| S17 | 5.32 | 4.84 | 63.01 | 0.63 | 0.21 | 0.02 | 0.07 | 0.02 | 0.02 |
| S18 | 6.93 | 5.34 | 57.38 | 0.47 | 0.11 | 0.02 | 0.07 | 0.02 | 0.02 |
| S19 | 5.62 | 5.52 | 65.82 | 0.66 | 0.14 | 0.04 | 0.12 | 0.04 |
3 kev sib tham
Chromatographic kev txhim kho
Thaum lub sij hawm ua kom zoo ntawmChromatographic mob, ob peb lub xov tooj txawb xov tooj sib piv tau muab piv, suav nrog:
Methanol / Acetonitrile - 0. 2% phosphoric acid daws
Methanol / Acetonitrile - 0}.% Cov Qauv Acid
Methanol / Acetonitrile - 0. 2% formic acid daws
Methanol / Acetonitrile - 0. 5% formic acid daws
Nws tau pom tias thaum sivAcetonitrile - 0. 2% phosphoric acid dawsRaws li cov theem mobile, tusBaseline tsom me ntsis sib txawvCov. Thaum sivMethanol - 0. 1% formic acid daws, tussib cais ntawm cov chromatographic peaks yog tus tsis zooCov. Thaum kawg,Acetonitrile - 0. 5% formic acid dawsraug xaiv los ua mobile theem vim nwsZoo dua cov duab thiab sib cais ua haujlwm.
RauKev pom wavelength thiab kem ntsuas kub, tom qab txiav txim siabYooj yim ntawm kev ua haujlwm thiab raug, qhov kev tshawb pom yoj ntawm330 nmmuab los ntawmPharmacopoeiatau xaiv rauEchinacoside thiab Verbualcoside, thiab aKab kub ntawm 30 degreetau pom tias muaj kev nqus tau zoo rau tag nrho rau lub cheeb tsam.
Kev xaiv ntawm kev ntsuam xyuas cov ntsuas rau kev haus cawv-soaked thiab ciContancheDecoction daim
Hauv kev xaivIntrinsic Cov Qauv Kev Ntsuam Xyuas Zoo, ob qho tib sikev nkag siab thiab kev tshawb fawb tautau hais txog. Tus xaivCim Cheebtsamsuav nrog:
Cov ntsiab lus siabxws liEchinacoside thiab Verbualcoside
Cov tebchaw nrog cov ntsiab lus tseem ceeb hloov ua ntej thiab tom qab ua tiav, xws liisAstoverebascoside, 2'-acetylverboscoside, thiab cistanoside a
Kev tiv thaiv biologically nquag, xws liTubuloside a
Rau xamNyhav coefficients thiab cov qhab nia, tusAHP-kev thuam txoj kev hnyav txoj kevtau siv, uas siv tau zoo ua keKev Kawm thiab Cov Hom Phiaj, txo cov kev tsis txhob muaj tseeb thaum ua kom muaj zogCov Ntaub Ntawv Kev Ruaj Ntseg thiab hloov siabCov. Txoj kev cuam tshuam no minimizes cuam tshuam, kom paub tseebKev tshawb fawb thiab cov txiaj ntsig ruaj khov, thiab txhim kho kev ntseeg tau ntawm ntau qhov ntsuas tau nkag mus zoo.
Kev tshuaj xyuas kev ua cov txheej txheem rau dej cawv-soaked thiab ciContanche
Txoj kev tshawb no tshawb xyuas covTshuabntawmcawv-soaked thiab ciContanchesiv aIb Leeg-Qhov Tseeb SimTxhawm rau ntsuas cov teebmeem ntawm:
Hom thiab cov dej cawv
Soaking ntev
Ci Roasting Kub
Lub sij hawm ci
Nyhuv ntawm cov cawv sib txawv
Ib qho kev sib piv ntawmHuangjiu (cov nplej daj), mijiu (rice cawv), thiab baijiu (cawv distilled cawv)pom tias nyob rau hauvHuangjiu ua cov haujlwm, tusRau feem tiv thaiv cov ntsiab lus tau siab dua hauv mijiu, thiabEchinacoside thiab Verbualcoside tau ruaj khov dua, zoo sib xws nrog cov kev tshawb nrhiav yav dhau los[7,8].
Ntxim ntawm cov dej cawv kom muaj nuj nqis
TusPharmacopoeiasau npe aHuangjiu-rau-ContanchePiv ntawm 10: 2 txog 10: 3Cov. Txawm li cas los xij, thaum siv a10: 1 piv rau hauv txoj kev kawm no, tusEchinacoside thiab Verbascoside Cov Ntsiab Lus tau ua siab duatshaj li cov uas tau siv cov tshuaj pharmaceopoeia cov cawv haus cawv.
Nyhuv ntawm soaking sij hawm
Raws liTsoos suav tshuaj noj cov tshuaj, "So rau peb hnub thiab ci kom zoo" yog qhov pom zoo. Yog li ntawd, lub sij hawm soaking tau sim ntawm6 txog 72 teev.
Ntxim ntawm ci kub thiab sijhawm
Lub sij hawm"Ci" (焙, Bèi)Kev lig kev cai hais txog ci ntawm rhuabCeramic Pobzeb, uas tuaj yeem ncav cuag qhov kub mus txog200 degreeCov. Tus"Anhui Provincial Ua Cov Qauv"Qhia kom meej tias ci yuav tsum sivTsawg Tsawg, yog li qhov kub thiab txias(85 ± 5) degree rau (185 ± 5) degreetau sim.
Txij liNtev lub sijhawm ua rau kom ploj ntau dua ntawm cov khoom siv volatile, ci caij ntawm20 txog 40 feebtau xaiv, thiab covQhov Zoo Tshaj Plaws Parameter RangesRau txhua qhov kev txiav txim siab tau txiav txim siab.
Kev ua kom zoo ntawm kev ua cov tsis siv siv AHP-thuam thiab orthogonal tsim
Siv covAHP-SCROPY LUJ LUJ LIM, cov coefficients ntawm txhua tusTshuaj ntsuam xyuas qhov ntsuasnyob rau hauvcawv-soaked thiab roasted kev txoj kevtau txiav txim siab. Tom qab ntawd,Orthoogonal sim tsimtau siv rau kev ua kom zooUa tsis, ua rau tusKev Ua Haujlwm Kawg:
100 g ntawm kev limContanche
10 g ntawm Huangjiu
Soaking rau 26 teev
CIS RAU (155 ± 5) Qib rau 30 feeb
Cua txias lawmkom tau txais cov khoom kawg
Tsim tsaCov Qauv ZooRau cawv-soaked thiab ciContancheDecoction cov yog:
Cov ntsiab lus ya raws tsawg dua los yog sib npaug rau 9.56%
Tag nrho cov hmoov tshauv cov ntsiab lus tsawg dua lossis sib npaug rau 8.15%
Ethanol-soluble extract cov ntsiab lus ntau dua los yog sib npaug rau 46.54%
Tag nrho cov echinacoside thiab verbascoside cov ntsiab lus ntau dua los yog sib npaug rau 0. 59%
Kev Sib Tw thiab Kev Tshawb Fawb Txog Yav Tom Ntej
Sim cov txiaj ntsig tau pomtseem ceeb sib txawv ntawm cov sib txawv, uas yuav raug ntaus nqi rauKev hloov pauv hauv qhov nyoosContanchekhoom.
Tam sim no,ntau cov nyom nyobHauv kev kawm txogContancheUa, xws li:
TusCov yam ntxwv zoo tshaj plawsntawmCawv-ua tiavContancheDecoction daim, uas yuav tsum yogtsaus xim av, ci ntsa iab, me ntsis mos nyob rau hauv kev ntxhib los mos, qab zib hauv saj, thiab me ntsis aromatic nrog cawv
Qhov sib txawv ntawm kev ntxhib los ntawmcawv-steamed, dej cawv-stewed, thiab roasted decoction pieces, qhov twgsteamed thiab stewed daim yuav yog softer, thaum ci ci yog qhov nyuaj
Txawm yogCov ntawv sau txawvsib haum rauIntrinsic Zoo Hloov, xav tau kev tshawb fawb ntxiv
Kev Sib Tw thiab Kev Tshawb Fawb Txog Yav Tom Ntej
Txawm hais tias txoj kev kawm no ua tau zoo rau covcawv-soaked thiab ciContancheTxoj kev uathiab tsim cov qauv zoo,tseem ceeb sib txawvtau pom ntawmCov Khaub Ncaws Sib Txawvntawm cov txheej txheem decoction daim. Txoj kev sib txawv no yuav yog vimKev xam pom qhov zoo sib txawv hauv kev nyoosContanchekhoom, Qhia txog qhov xav tau kom muaj dua tshiab hauv kev txheeb xyuas cov khoom siv roos thiab ua cov txheej txheem.
Tam sim no,ntau cov nyom nyobHauv kev kawm txogContancheUa, xws li:
TusCov yam ntxwv zoo tshaj plawsntawmCawv-ua tiavContancheDecoction daim, uas yuav tsum yogtsaus xim av, ci ntsa iab, me ntsis mos nyob rau hauv kev ntxhib los mos, qab zib hauv saj, thiab me ntsis aromatic nrog cawv
Qhov sib txawv ntawm kev ntxhib los ntawmcawv-steamed, dej cawv-stewed, thiab roasted decoction pieces, qhov twgsteamed thiab stewed daim yuav yog softer, thaum ci ci yog qhov nyuaj
Txawm yogCov ntawv sau txawvsib haum rauIntrinsic Zoo Hloov, xav tau kev tshawb fawb ntxiv
Txoj kev kawm no muaj ascorientific rau kev txhim kho cov cawv-soaked thiab ciContancheKev Ua Cov Txheej, tab sis ntxiv kev tshawb nrhiav xav tau rautxhim kho cov qauv thiab kom cov pob-rau-batch sib xws.
